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[Studies of pure human pancreatic juice]. [纯人类胰液的研究]。
H Weber, W Dummler, S Liebe

The test-combination from Fa. Boehringer Mannheim GmbH for measuring citric acid present in foodstuffs is also suitable for use in human pancreatic juice. Accuracy, within-run and between-day imprecision are satisfactory. The detection limit of the method is at least 10 mumol citrate/l pancreatic juice. The limit can be reduced when exact additions of citrate are given to the pancreatic juice and then evaluated using regression analysis. The presence of Ca-ions and possibly also trypsin in the material to be tested does not interfere with the reaction. It is, however, necessary to remove proteins from the sample. Deproteinization can be performed either by ultrafiltration or with perchloric acid.

来自Fa的测试组合。勃林格曼海姆有限公司用于测量食品中存在的柠檬酸,也适用于人体胰液。精度,运行内和日间不精度令人满意。本方法的检出限为至少10 mol柠檬酸盐/l胰液。如果在胰液中加入柠檬酸盐,然后用回归分析进行评价,则可以降低这一限度。待测材料中存在的钙离子和可能存在的胰蛋白酶不会干扰反应。然而,有必要从样品中去除蛋白质。脱蛋白可通过超滤或高氯酸进行。
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引用次数: 0
[Clinical relevance of the determination of TSH-binding inhibitory immunoglobulins (TBII) in patients with immunogenic hyperthyroidism in methimazole therapy]. [甲巯咪唑治疗免疫原性甲亢患者tsh结合抑制性免疫球蛋白(TBII)测定的临床意义]。
H J Heberling, B Bierwolf, E Kuhlmann, T Klugmann, P Dietel, S Fischer

In 38 patients with immunogenic hyperthyroidism a follow-up was performed to estimate the value of TBII before, during and after methimazole therapy. Before therapy increased TBII were detectable in 37 patients (94.4%). After 12 months methimazole therapy 25 patients had TSH-receptor antibodies (66%) within the normal range. In 13 patients positive antibody titres were found. In most cases persistence of increased TBII-values during drug treatment was an indicator of the persistence of active hyperthyroidism (10 of 13 patients). In the rule a disappearance of TBII-activity was combined with a functional remission (22 of 25 patients). Prolonged demonstration of TBII-activity in conjunction with persistence of hyperthyroidism should lead to ablative measures. In contrast to this medical therapy should be finished in patients with immunological and functional remission. Though in the further follow-up a recurrence of the immunological base of the disease with a functional and clinical relapse is possible.

对38例免疫原性甲状腺功能亢进症患者进行随访,以评估甲巯咪唑治疗前、期间和之后的TBII值。治疗前,37例患者(94.4%)检测到TBII升高。甲巯咪唑治疗12个月后,25例患者tsh受体抗体(66%)在正常范围内。13例患者抗体滴度均为阳性。在大多数病例中,药物治疗期间持续升高的tbii值是活动性甲亢持续存在的一个指标(13例患者中有10例)。在规则中,tbii活性消失与功能缓解相结合(25例患者中有22例)。长时间的tbii活动与持续的甲状腺功能亢进应该导致消融措施。与此相反,药物治疗应在免疫和功能缓解的患者中结束。虽然在进一步的随访中,疾病的免疫基础复发与功能和临床复发是可能的。
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引用次数: 0
[Alimentary iodine deficiency in East Germany and its reflection in thyroid parameters following the initiation of struma prevention]. [东德的食源性碘缺乏症及其在甲状腺参数中的反映]。
K Bauch

By regional and countrywide studies carried out in the past 10 years the occurrence of endemic goitre and iodine deficiency (ID) of grade II has been demonstrated in 1981. In 1983 till 1985 an iodized salt (25 mg KI/kg) was offered mainly in the southern counties of the G.D.R. This measure has been followed by the production of 84% of the whole paket salt iodized with stable potassium iodate (32 mg KIO3/kg) since July 1985 and delivered in all the G.D.R. Nevertheless, in the end of 1985 the persistence of iodine deficiency of grade II has yet to be stated by low renal iodine excretion, low iodine content of milk and human milk post partum. The reference values of TT3 (0.84-3.76 nmol/l) and TT4 (41.8-137.3 nmol/l) assessed in 1985 for the G.D.R. are to be interpreted before the background of an iodine deficiency of grade II.

在过去10年中进行的区域和全国范围的研究表明,地方性甲状腺肿和II级碘缺乏症(ID)的发生在1981年。1983年至1985年碘盐KI(25毫克/公斤)提供主要在南部县G.D.R.之后这种方法生产的84%的整个寄盐含碘稳定碘酸钾(KIO3 32毫克/公斤)1985年7月以来和交付的所有G.D.R.然而,1985年底,持久性的碘缺乏二级尚未规定的低肾排泄碘,碘含量低的牛奶和母乳产后。1985年评估的TT3参考值(0.84-3.76 nmol/l)和TT4参考值(41.8-137.3 nmol/l),应在二级缺碘背景之前进行解释。
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引用次数: 0
[Effect of various liver diseases on the activity of 2 subtypes of cytochrome P-450]. [各种肝脏疾病对细胞色素P-450 2亚型活性的影响]
A Balogh, H J Simon, M Reinhardt, F K Splinter, A Traeger

The elimination of caffeine from the plasma and the elimination of metamizol-metabolites in urine were determined in 37 patients with different liver diseases. In severe liver diseases the demethylation of caffeine as well as the metabolism of metamizol is significantly reduced. The extent of reduced elimination capacity depends on the severity of the disease rather than on the type of disease. In patients with liver cirrhosis the determination of synthesis capacity and of humoral activity (s. tab. I) is suitable to evaluate the capacity of the cytochrome P-450 system. In noncirrhotic diseases only the activity of liver disease (tab. I.) determines the extent of reduced biotransformation capacity. Beside biotransformation-phase I, the acetylation--phase II biotransformation--also appears to be reduced.

对37例不同肝病患者血浆中咖啡因的消除和尿中甲胺醇代谢物的消除进行了测定。在严重的肝脏疾病中,咖啡因的去甲基化以及metamizol的代谢显著减少。消除能力降低的程度取决于疾病的严重程度,而不是取决于疾病的类型。肝硬化患者的合成能力和体液活性的测定(见表1)。I)适合于评价细胞色素P-450体系的容量。在非肝硬化疾病中,只有肝脏疾病的活动性。1)确定生物转化能力降低的程度。除了生物转化阶段I,乙酰化-阶段II生物转化-似乎也减少了。
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引用次数: 0
Characterization of primary biliary cirrhosis (PBC) specific mitochondrial determinants by immunoblotting. 原发性胆汁性肝硬化(PBC)特异性线粒体决定因素的免疫印迹表征。
Pub Date : 1987-01-01 DOI: 10.1016/s0168-8278(85)80243-9
M. Manns, G. Gerken, C. Trautwein, M. Meuer, T. Porally, H. Dienes, K. Meyer zum Büschenfelde
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引用次数: 7
[The splendor and misery of Whipple's disease]. [惠普尔病的辉煌与痛苦]。
H J Klugmann, B Wohlgemuth, E Kupsch

On the basis of two cases diagnosed by ourselves, and a review of literature the problematic Whipple's disease is demonstrated. The treatment of this rare disease is very successful but it is too seldom included in the differential diagnosis of arthralgia and malabsorption. Therefore fatal false diagnosis results very often.

本文结合自己诊断的两例病例,并对文献进行复习,证实了疑心病。这种罕见疾病的治疗非常成功,但它很少被纳入关节痛和吸收不良的鉴别诊断。因此,致命的错误诊断结果非常频繁。
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引用次数: 0
[Pathogenesis of hyperoxalurias and calcium oxalate calculi in intestinal diseases]. [肠道疾病中高尿酸和草酸钙结石的发病机制]
G Müller, W Schütte, T Möller

Hyperoxaluria and calcium oxalate lithiasis have a multifactorial genesis in bowel diseases. The augmented synthesis of oxalic acid in the liver is of minor importance. A diminished bacterial degradation is to asses up to day only hardly. The increasing absorption is of highest importance by deficient production of calcium oxalate in the intestinal tract and by increasing permeability of colonic mucosa above all in steatorrhoea or in patients with augmented calcium absorption. The crystallization of calcium oxalate in the urine is promoted by shortage of vitamin A, citrate, zinc or magnesium.

高草酸尿症和草酸钙结石在肠道疾病中有多因素的发生。肝脏中草酸合成的增强是次要的。一种减少的细菌降解是迄今为止唯一难以评估的。增加的吸收是最重要的是缺乏生产草酸钙在肠道和增加结肠粘膜的通透性,尤其是在脂肪变性或钙吸收增强的患者。由于缺乏维生素A、柠檬酸盐、锌或镁,尿液中草酸钙的结晶会被促进。
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引用次数: 0
[Histologic and cell kinetic findings in stomach ulcer of different healing times]. [不同愈合时间胃溃疡的组织学和细胞动力学表现]。
E Zinsser, G Machnik, K H Stiller

The 3-month healing rate in gastric ulcer patients treated on an outpatient basis and with no H2 blockers is only some 40%. In order to be able to improve therapy at an early stage, prognosis-relevant parameters were searched for also among histological findings. The semiquantitatively evaluated morphological parameters from 62 gastric ulcer patients of a case control study were subjected to a computerized multivariate analysis. Accordingly, at the stage of healing of gastric ulcer, the features 'complete intestinal metaplasia' and 'incomplete intestinal metaplasia' separate with the aid of a non-elementary discriminant function between healing in an appropriate period (3 months) and delayed healing. Histoautoradiographic in vitro examinations of 50 gastric ulcer cases reveal that the mucous membrane around an ulcer healing with delay has a significantly higher cell labeling rate than the gastric mucosa of control persons. The influence of a reflux of bile from the duodenum is discussed, as there is no correlation with the degree of chronic gastritis.

在门诊治疗的胃溃疡患者中,3个月的治愈率仅为40%左右。为了能够在早期阶段改善治疗,预后相关参数也在组织学发现中进行了搜索。在一项病例对照研究中,对62例胃溃疡患者的半定量形态学参数进行了计算机化的多变量分析。因此,在胃溃疡愈合阶段,“完全肠化生”和“不完全肠化生”的特征在适当时期(3个月)愈合和延迟愈合之间的非基本判别函数的帮助下分开。50例胃溃疡患者的组织放射自显影体外检查显示,溃疡延迟愈合者周围粘膜细胞标记率明显高于对照组。十二指肠胆汁反流与慢性胃炎的程度无关,因此我们讨论了胆汁反流的影响。
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引用次数: 0
Characterization of primary biliary cirrhosis (PBC) specific mitochondrial determinants by immunoblotting. 原发性胆汁性肝硬化(PBC)特异性线粒体决定因素的免疫印迹表征。
M Manns, G Gerken, C Trautwein, M Meuer, T Porally, H P Dienes, K H Meyer zum Büschenfelde

Antimitochondrial antibodies (AMA) are detected in up to 100% of patients with primary biliary cirrhosis (PBC); subtypes of AMA are disease specific. Sera from 21 patients with PBC and from 50 patients with various hepatic and non-hepatic diseases were tested for AMA by indirect immunofluorescence, radioimmunoassay for PBC-specific subtype anti M2 and characterized by western blot analysis and agarose-IEF-immunoblotting. Sera from patients with PBC reacted with up to 7 different mitochondrial polypeptides on western blots, mol. wt. 24,000-62,000 dalton. Sera from 50 patients with various hepatic and non-hepatic diseases did not react with these polypeptides. Sera with other AMA subtypes were included in this study (anti M1, anti M3, and anti M5). These mitochondrial polypeptides were associated with inner mitochondrial membranes (mitoplasts). Sonification led to a solubilization of several mitochondrial polypeptides (p 62, p 48, p 40, p 24). On agarose-IEF-immunoblotting sera from patients with PBC and 3 sera from patients with AMA positive cholestatic CAH but no other sera reacted with a protein band at pI 4.0; seven PBC sera reacted in addition with a protein band at pI 4.4. Western blot and agarose-IEF-immunoblotting are sensitive and specific tools to identify and characterize mitochondrial target antigens in PBC. Furthermore these techniques allow to study the clinical relevance of the heterogeneity of AMA in cholestatic liver disease.

在高达100%的原发性胆汁性肝硬化(PBC)患者中检测到抗线粒体抗体(AMA);AMA亚型是疾病特异性的。本文采用间接免疫荧光法检测21例PBC患者和50例各种肝脏和非肝脏疾病患者血清中AMA的含量,用PBC特异性抗M2亚型放射免疫法检测,并用western blot分析和琼脂糖- ief免疫印迹法检测。PBC患者的血清在western blots上与多达7种不同的线粒体多肽反应,摩尔重量为24,000-62,000道尔顿。50例各种肝脏和非肝脏疾病患者的血清对这些多肽没有反应。其他AMA亚型血清(抗M1、抗M3和抗M5)也被纳入本研究。这些线粒体多肽与线粒体内膜(有丝分裂体)有关。超声导致了几种线粒体多肽的溶解(p 62, p 48, p 40, p 24)。PBC患者的琼脂糖- ief免疫印迹血清和AMA阳性的胆汁淤积CAH患者的血清,但没有其他血清与pI 4.0的蛋白带反应;7种PBC血清在pI 4.4处与蛋白带发生反应。Western blot和琼脂糖- ief免疫印迹是鉴定和表征PBC线粒体靶抗原的敏感和特异性工具。此外,这些技术允许研究AMA在胆汁淤积性肝病异质性的临床相关性。
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引用次数: 0
[ST elevation in the initial phase of myocardial infarct--a sequela of acute coronary occlusion of an expression of neurohumorally-induced metabolic processes?]. 【心肌梗死初期ST段抬高——急性冠状动脉闭塞引起的神经体液诱导代谢过程表达的后遗症?】
G Jentsch, B Kottwitz

The artificial coronary artery occlusion results immediately in ST-segment elevation (ST-E), therefore the ST-E in acute myocardial infarction is considered to be the result of acute coronary occlusion. But in the early phase of myocardial infarction a significant correlation between ST-E and occlusion of the coronary artery does not exist. The early ST-E seems to be predominantly the consequence of sympathico-adrenergic induced metabolic processes in the ischemic myocardium. The beta receptor blockade during the first 90 minutes after the onset of infarction decreased the ST-E by 73 +/- 6% within one hour. Treatment with beta receptor blockers in the acute phase in all patients with first anterior wall infarcts accompanied with ST-E leads to a favorable prognosis. Early ST-E can therefore be considered as a sign of sympathico-adrenergic induced changes in myocardial metabolism. Thus the beta receptor blockade in the acute phase (of anterior wall infarctions) seems to be therapeutically indicated.

人工冠状动脉闭塞会立即引起st段抬高(ST-E),因此急性心肌梗死的ST-E被认为是急性冠状动脉闭塞的结果。但在心肌梗死早期,ST-E与冠状动脉闭塞之间不存在显著相关性。早期ST-E似乎主要是交感神经-肾上腺素能诱导的缺血心肌代谢过程的结果。在梗死发作后90分钟内β受体阻断使ST-E在1小时内降低73 +/- 6%。所有伴有ST-E的第一前壁梗死患者在急性期接受受体阻滞剂治疗可获得良好的预后。因此,早期ST-E可以被认为是交感神经肾上腺素能引起的心肌代谢变化的标志。因此,在急性期(前壁梗死)β受体阻断似乎是有治疗意义的。
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引用次数: 0
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Deutsche Zeitschrift fur Verdauungs- und Stoffwechselkrankheiten
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