Doklady Biological Sciences最新文献

Activities of key enzymes of energy and carbohydrate metabolism (cytochrome c oxidase (COX), lactate dehydrogenase (LDH), aldolase, glucose 6-phosphate dehydrogenase (G6PDH), and 1-glycerophosphate dehydrogenase (1-GPDH)) were studied in pink salmon Oncorhynchus gorbuscha (Walb.) smolts from the White Sea in a cage experiment simulating the transition from a freshwater to a marine environment. A decrease in COX, G6PDH, and 1-GPDH activities and an increase in LDH and aldolase activities were observed in juveniles with an increase in water salinity. Based on the findings, a redistribution of energy substrates between the reactions of aerobic and anaerobic metabolism towards higher anaerobic ATP synthesis was assumed for pink salmon. This may indicate that adaptive mechanisms rearrange metabolism to provide energy for osmoregulation in pink salmon juveniles when the salinity changes in their habitat.

Activities of energy and carbohydrate metabolism enzymes (cytochrome c oxidase (COX), pyruvate kinase (PK), glucose 6-phosphate dehydrogenase (G6PD), glycerol 1-phosphate dehydrogenase (G1PDH), lactate dehydrogenase (LDH), and aldolase) were studied in rainbow trout Oncorhynchus mykiss Walb. fish grown in aquaculture in North Ossetia-Alania after introducing a regime with 24-h lighting and night feeding. COX and PK activities in the liver of fish from the experimental group were found to be significantly higher than in control fish, indicating an increase in aerobic ATP synthesis. Aldolase activity in organs of fish grown with 24-h lighting was lower than in the control fish, indicating a decrease in carbohydrate utilization in glycolysis in muscles and a lower intensity of gluconeogenesis in the liver. The differences made it possible to assume that the introduction of 24-h lighting and night feeding changed energy and carbohydrate metabolism to facilitate biosynthetic processes and, therefore, weight gain in fish.

Numerous studies of microorganisms isolated from the surface of cultural heritage objects, including library documents, are regularly carried out in different countries. Although the micromycete composition in each case varies, some species are constantly isolated. The structure of micromycete communities that inhabit library documents was studied in 57 cities of Russia located in seven federal districts (Northwestern, Central, Southern, Volga, Ural, Siberian, and Far Eastern). Micromycetes of 95 species from 32 genera were isolated and identified. The mycobiota of the library documents represented by Ascomycota has more than 90% of the species richness; Mucoromycota make up 3-9% and Basidiomycota, 3-4%. The Aspergillaceae family was the leading family: it accounted for 48.5-67.3% of the total species richness. In all regions, species diversity is moderate: the Shannon index ranged from 2.7 to 3.3. The Mcintosh species richness index is high everywhere (48-126), except in the Ural district (15.3). The McIntosh (0.76-0.84) and Pielow (0.80-0.91) dominance indices indicate a high level of species evenness in the mycobiota. The obtained values demonstrate the stability the document mycobiota in libraries from different regions. Significant species similarity between the districts was revealed by calculation of binary coefficients: the Jaccard coefficient was from 0.44 to 0.60; the Sørensen's qualitative measure of similarity was from 0.63 to 0.75; the quantitative similarity measure of Sørensen was from 0.44 to 0.71, and Morisita-Horn was from 0.66 to 1.0. The groups of dominant species in different regions are quite similar. The study of the ecological diversity of mycobiota on library books demonstrated the moderate diversity and the stability of the community. A high degree of similarity of taxonomic structures was established regardless of the climate conditions of the regions. Cosmopolitans characterized by high frequency of occurrence formed the major core of the library book mycobiota: Alternaria alternata, Aspergillus niger, Cladosporium cladosporioides, Mucor plumbeus, and Penicillium aurantiogriseum.

In the Plyussky district of the Pskov region, an experiment was carried out to infect birch trees with the Chaga pathogen Inonotus obliquus in an 18-year-old postagrogenic birch forest. After 10 years of the experiment, sterile bodies of Chaga were found on six out of ten trees, swelling of the bark was found in one tree, and one tree died from Phellinus nigricans. Infected trees continued to grow at a rate no different from the rest of the forest. Sterile growths were mainly located above the inoculation site. The study showed that the production of Chaga raw materials can be considered as an additional form of forest management in the Non-Black Earth Region of Russia.

The wild nature monument "184-year-old oak tree in the village of Irinovka" (Leningrad oblast, Russia), better known in local historical literature as "Irinovsky Oak," was officially opened in 2013. It is represented by a separate pedunculate oak tree (Quercus robur), planted in 1829 and preserved in satisfactory condition at 194 years of age. This paper presents data from a survey of the shoot system of the tree investigated. A total of 12 fungal species were registered (Cladosporium herbarum, Colpoma quercinum, Coryneum depressum, Diatrypella quercina, Erysiphe alphitoides, Hyphoderma setigerum, Laetiporus sulphureus, Mar-chandiomyces corallinus, Peniophora quercina, Trichoderma viride, Sphaerulina quercicola, Vuilleminia comedens). The lichenicolous species Marchandiomyces corallinus was reported as a new find to the Leningrad oblast. It was shown that the shoot system of the tree investigated is associated with a complex of Quercus robur-adapted and coadapted species, among which such necrotrophs as Sphaerulina quercicola and Coryneum depressum and such pathogenic saprotrophs as Vuilleminia comedens and Colpoma quercinum, as well as saprotrophs (Diatrypella quercina, Peniophora quercina), dominated. This species complex ensures a continuous process of the crown's thinning.

The cranial and mandibular fragments of the medium-sized canid from the Early Pleistocene locality of Taurida cave (about 1.8-1.5 Ma) in Crimea are described and included in the hypodigm of the species Canis etruscus Forsyth Major, 1877, a common member of the Late Villafranchian fauna of the Circum-Mediterranean region. This species is reported for the first time from the Northern Black Sea region. The discovered evidence reinforce previous published idea regarding the periodically use of the Taurida cave by canids and other large predators as a den and shelter.

Electroconvulsive therapy (ECT) is an effective treatment method for depression therapy. It produces a number of biological effects, including neurotrophic factors regulation. In the present paper, we investigated the ECT response in depressed rats subjected to the variable frequency ultrasound (20-45 kHz) and examined the contribution of brain-derived neurotrophic factor (BDNF) expression changes to the observed effects. The obtained results reflect the therapeutic potential of ECT for the treatment of depressive-like state in rodents and indicate the role of BDNF in these processes. In future research, it is necessary to investigate the relationship between neurotrophin and structural changes and to study other neurotrophic biomarkers that may be associated with the development of depression-like state and the therapy response.

A parasitological analysis was carried out with 29 samples of dog coprolites, soil, and manure obtained from the cultural layer of the Mangazeya settlement (66°42´N, 82°16´E), which dates back to 1601-1670 (end of the Late Holocene). Eggs of the nematode Dioctophyme renale (Goeze, 1782), which infests the kidneys of carnivores, were found in coprolites of dogs (Canis familiaris L., 1758) for the first time in Northwestern Siberia. The nematode species has not been detected in the modern helminth fauna of the region. The giant kidney worm could have entered the region with dogs brought by Russian migrants from European Russia during the development of Siberia. However, the introduction of the helminth did not lead to the formation of a new stable natural focus of dioctophymiasis.

The work contains a comparative analysis of methods for storing pure cultures of macrofungi. The study used 20 species of macrofungi from various taxonomic and ecological-trophic groups. Storage was carried out using five methods: serial subculturing, storage under a layer of distilled water and three cryopreservation protocols: a protocol using blocks of agar medium, a "perlite protocol," and a "grain protocol." For the selected cryostorage methods, various cryoprotective compounds (glycerol, trehalose) were used. Radial growth rate was used as a criterion for the state of crops. The values of the radial growth rate obtained immediately after isolation of the pure culture were chosen as the control. It has been shown that the most favorable for preserving the physiological activity of cultures are the storage method under a layer of distilled water, "perlite" and "grain" cryopreservation protocols.

Significant changes in lactobacillus-derived extracellular membrane vesicles, which transfer lipids, polysaccharides, proteins, and nucleic acids, were for the first time observed to accompany the development of resistance to antibiotics (amoxicillin and clarithromycin) in vitro in the probiotic strain Lactiplantibacillus plantarum 8p-a3. The changes occur together with large-scale genome rearrangements, changes in the profile of phenotypic sensitivity to antimicrobials of various groups, and evolution of virulence. Changes in vesicles affected their structure, composition, and activity against biofilms of opportunistic bacteria. The data provide for a better understanding of the molecular mechanisms of microbial survival under selective pressure of antimicrobials, the functional potential of probiotic vesicles, and probiotic safety assessments.