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Innovative approaches for immunodetection of proteic binders in art 艺术中蛋白质结合物免疫检测的创新方法
Pub Date : 2017-01-01 DOI: 10.18236/ECONS5.201708
C. Salvador, A. Branco, A. Candeias, A. T. Caldeira
The characterization and identification of the proteinaceous compounds in a complex and multi-layered painting is crucial for studying the technique used by the artist and for conservation and restoration purposes. These organic compounds, such as animal glues, milk and egg, have a particular importance since they are widely used as binders and adhesives in paintings. Proteins are typically detected by methods like chromatographic and proteomic techniques. However, the immunodetection approach shows to be a powerful method in protein analysis. In this study immunologic techniques, based on enzyme-linked immunosorbent assay (ELISA), were used in order to identify different proteic binders used in easel paintings. The methodology based on indirect ELISA allows the detection of the target antigen in paint models microsamples. These approach is very promising with regard to the possibility of apply these methodology in the detection of proteinaceous binders in real samples.
在一幅复杂的多层绘画中,蛋白质化合物的表征和鉴定对于研究艺术家使用的技术以及保护和修复目的至关重要。这些有机化合物,如动物胶、牛奶和鸡蛋,具有特别重要的意义,因为它们被广泛用作绘画中的粘合剂和粘合剂。蛋白质通常通过色谱和蛋白质组学技术等方法检测。然而,免疫检测方法在蛋白质分析中显示出一种强大的方法。在这项研究中,基于酶联免疫吸附测定(ELISA)的免疫技术被用于鉴定架上绘画中使用的不同蛋白质粘合剂。基于间接ELISA的方法允许检测涂料模型微样品中的靶抗原。这些方法对于在实际样品中检测蛋白质结合剂的可能性应用这些方法是非常有希望的。
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引用次数: 6
The Application of Colour Management to a Photographic Record for the Conservation of Scientific Collections: Anatomical Wax Models 色彩管理在科学收藏保存摄影记录中的应用:解剖蜡模型
Pub Date : 2017-01-01 DOI: 10.18236/ECONS5.201705
A. Sánchez, L. Castelo
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引用次数: 0
Colorimetric measurements of different variants of verdigris: powder and bound pigments 铜绿不同变体的比色测定:粉末和结合色素
Pub Date : 2017-01-01 DOI: 10.18236/ECONS5.201706
N. Sancho, M. S. Andrés, S. Santos, J. M. D. L. Roja
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引用次数: 0
Type II Super Lattice (T2SL) imaging technology for infrared reflectography of polychrome works of art II型超晶格(T2SL)成像技术用于彩色艺术品的红外反射
Pub Date : 2017-01-01 DOI: 10.18236/ECONS5.201709
A. Cosentino
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引用次数: 1
Pigments from a paint box from the atelier of the Portuguese painter of the early twentieth century, Aurélia de Sousa 20世纪早期葡萄牙画家奥拉西亚·德·索萨画室里的颜料盒
Pub Date : 2017-01-01 DOI: 10.18236/econs5.201707
María Aguiar, A. Calvo, António João Cruz, A. Candeias, J. Mirão
Painting materials can provide relevant information about the availability offered to artists within specific periods of time and particular geographical locations, contributing to understand painter´s choices, preferences and limitations. The nineteenth century was, by far, the moment where an unprecedented diversity on pigments and lakes occurred, due the discovery of new metals and the huge advances on inorganic chemistry and on the synthesis of artificial dyestuffs. A painting box containing 36 oil tubes was recovered by the family of the Portuguese painter, Aurélia (1866-1922) atelier, at Quinta da China, Porto. The atelier was shared with her sister, Sofia de Sousa (1870-1960), who ceased painting when Aurélia died. They both studied at the Academia Portuense de Belas Artes, at Porto and later, at the turnover of the 19th century, at the Academie Julian, in Paris. of namely pigments, extenders and lake substrates, SEM-EDS, in to the painting materials actually used previously determined the study of
绘画材料可以提供艺术家在特定时期和特定地理位置的可用性的相关信息,有助于了解画家的选择,偏好和局限性。到目前为止,由于新金属的发现以及无机化学和人工染料合成的巨大进步,19世纪出现了前所未有的颜料和湖泊的多样性。葡萄牙画家奥尔萨里亚(1866-1922)的家人在波尔图的金塔达中国发现了一个装有36根油管的绘画盒。她的妹妹索菲亚·德·索萨(Sofia de Sousa, 1870-1960)在她去世后停止了绘画。他们都曾在波尔图的葡萄牙艺术学院(Academia Portuense de Belas Artes)学习,后来在19世纪的交替时期,在巴黎的朱利安学院(Academie Julian)学习。即颜料、填充剂和湖底物,SEM-EDS,在对绘画材料实际使用的研究中预先确定
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引用次数: 0
Madder: from the plant to the museum objects Madder:从植物到博物馆的物品
Pub Date : 2017-01-01 DOI: 10.18236/ECONS5.201712
C. Mathe, J. Mazzitelli, C. Vieillescazes
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引用次数: 0
An Analysis of Alfredo Volpi’s Paintings by X-ray Fluorescence, Spectrophotometry and Technical Photography 用x射线荧光、分光光度法和技术摄影法分析阿尔弗雷多·沃尔皮的绘画
Pub Date : 2017-01-01 DOI: 10.18236/econs5.201701
E. K. Mori, E. A. D. Lama, M. Rizzutto, E. Kajiya, P. Campos
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引用次数: 0
Colour Change Caused by Dry Cleaning Process on Painted Surfaces 油漆表面干洗过程引起的颜色变化
Pub Date : 2017-01-01 DOI: 10.18236/econs5.201703
Silvia García Fernández-Villa, J. M. D. L. Roja, M. S. A. Moya
Over the last few years, the dry cleaning process has become an excellent alternative to solvent or water-based mixtures, which prevents diffusing the dirt into the painting structure during the cleaning process. The use of this kind of cleaning is especially effective on contemporary paintings as it prevents the solubilisation of additives such as the surfactants contained in polymeric dispersions (e.g. in acrylic and PVAc-based paints). In addition to some reported side effects that could be caused by dry cleaning processes on paintings (such as changes in the integrity of the paint or any residual materials which may be left behind) one parameter that should be tested is the colorimetric change seen on treated surfaces. In some cases, the use of certain dry cleaning materials can cause burnishing or roughening of the painted surface. This effect results in a change in brightness and chroma and subsequently to a change in the colour of the treated area, especially on dark shades. This article evaluates the colorimetric change of different kinds of painted surfaces caused by the dry cleaning process, such as gouache, commercial formulated acrylic, fish glue tempera, casein tempera, PVAc and primal acrylic paint. The cleaning materials tested include different kinds of sponge cloths, malleable materials and erasers used in the field of heritage conservation/restoration. The change in colour is measured by means of a spectrophotometer and the chromatic values are processed in a spreadsheet. The colorimetric changes have been calculated using the advanced equation recommended by CIEDE2000 and the chromatic values obtained have been studied following the standard recommendations.
在过去的几年里,干洗工艺已经成为溶剂或水基混合物的一个很好的替代品,它可以防止在清洗过程中污垢扩散到油漆结构中。这种清洗方法对当代绘画尤其有效,因为它可以防止聚合物分散体(如丙烯酸和聚氯乙烯基涂料)中含有的表面活性剂等添加剂的溶解作用。除了一些报道的可能由油漆干洗过程引起的副作用(例如油漆完整性的变化或可能留下的任何残留材料)之外,应该测试的一个参数是在处理过的表面上看到的比色变化。在某些情况下,使用某些干洗材料会导致涂漆表面抛光或变粗糙。这种效果导致亮度和色度的变化,随后导致处理区域的颜色变化,特别是深色。本文评价了水粉、市售配方丙烯酸、鱼胶蛋彩漆、酪蛋白蛋彩漆、聚氯乙烯和原丙烯酸漆等不同油漆表面在干洗过程中所引起的比色变化。测试的清洁材料包括不同种类的海绵布、可延展材料和用于文物保护/修复领域的橡皮擦。用分光光度计测量颜色的变化,并在电子表格中处理颜色值。使用CIEDE2000推荐的高级公式计算了比色变化,并根据标准建议研究了得到的色值。
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引用次数: 1
An Exercise in Fugitivity: Investigating the fading of red lake pigments 逃亡性的练习:研究红色湖色颜料的褪色
Pub Date : 2017-01-01 DOI: 10.18236/econs5.201702
D. Léonard
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引用次数: 0
The Painted Angels from the central drum of Convent of Christ Rotunda in Tomar - first results of the material and technical comparative study 托马尔基督圆厅修道院中央鼓上的彩绘天使——材料和技术比较研究的第一个成果
Pub Date : 2015-01-01 DOI: 10.18236/ECONS4.201510
M. Gil, A. Pestana, C. Miguel, A. Manhita, L. Dias, Ana Cardoso, J. Mirão, A. Candeias
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引用次数: 0
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