Pub Date : 2021-09-27DOI: 10.21608/ejrsa.2021.89718.1121
H. Hasan, S. Galal
{"title":"Reform of Hematopoietic, Apoptotic and Oxidative Disturbance Induced by Accumulated γ-Irradiation in Rat’s Bone Marrow via Curative Efficacy of Bradykinin Potentiating Factor Isolated from Bee Venom","authors":"H. Hasan, S. Galal","doi":"10.21608/ejrsa.2021.89718.1121","DOIUrl":"https://doi.org/10.21608/ejrsa.2021.89718.1121","url":null,"abstract":"","PeriodicalId":11658,"journal":{"name":"Egyptian Journal of Radiation Sciences and Applications","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87011021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-23DOI: 10.21608/ejrsa.2020.51426.1108
S. Shaban, K. El-Adham, W. Khalil
Accounting and controlling nuclear materials is the backbone for safeguards implementation and it is also an important factor for successful security undertakings. It manages the process of registering sensitive materials by accurately maintaining bookkeeping and following changes in material inventory (IAEA, 2008). Continuous measuring of nuclear material and facility operating data are employed to get up-to-date information. The accounting system is not only responsible for maintaining the bookkeeping of nuclear inventory, but also responsible for tracking their locations and movements (Gavron, 2001). The records and reports done by the accounting system are subject to auditing by the national and international inspection authorities through verification and measurement of nuclear materials to confirm correctness and completeness (Goldman, 1994).
{"title":"Fast Procedure for Aqueous Samples Investigation Using SEM and EDX for Nuclear Safeguards Purposes","authors":"S. Shaban, K. El-Adham, W. Khalil","doi":"10.21608/ejrsa.2020.51426.1108","DOIUrl":"https://doi.org/10.21608/ejrsa.2020.51426.1108","url":null,"abstract":"Accounting and controlling nuclear materials is the backbone for safeguards implementation and it is also an important factor for successful security undertakings. It manages the process of registering sensitive materials by accurately maintaining bookkeeping and following changes in material inventory (IAEA, 2008). Continuous measuring of nuclear material and facility operating data are employed to get up-to-date information. The accounting system is not only responsible for maintaining the bookkeeping of nuclear inventory, but also responsible for tracking their locations and movements (Gavron, 2001). The records and reports done by the accounting system are subject to auditing by the national and international inspection authorities through verification and measurement of nuclear materials to confirm correctness and completeness (Goldman, 1994).","PeriodicalId":11658,"journal":{"name":"Egyptian Journal of Radiation Sciences and Applications","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82908908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-10-23DOI: 10.21608/ejrsa.2019.13784.1074
M. Alkady, I. Ibrahim
AN ENDOGENOUS inhibitor of nitric oxide synthase, asymmetric dimethylarginine (ADMA), is elevated in patients with type 2 diabetes mellitus (T2DM) and has been related to atherosclerotic disease. The study aims at investigating the status of ADMA and nitric oxide (NO) and their possible correlation inpatients with T2DM with and without cardiovascular complicationsin comparison to normalcontrols. Forty patients with T2DM and 20 age, sex and body mass index (BMI)‑matched healthy controls were included in the study. The studied individuals were divided into Group1: healthy controls (n= 20), Group 2: T2DM patients without cardiovascular complications (n= 20) and Group 3: T2DM patients with evidence of cardiovascular complications (n= 20). Serum ADMA levels were determined by enzyme linked Immunosorbent Assay (ELISA). Serum nitric oxide was measured as stable end product, nitrite. Insulin was measured by immunoradiometric method. Serum ADMA levels showed a significant elevation while serum NO levels were significantly reducedin diabetic patients groups in comparison to controls. Diabetic patients with vascular complications showed highly significant increase in ADMA levels and pronounced decrease in NO compared to those without complications. In the group of vascular complications, the ADMA level was positively correlated with postprandial serum glucose and HbA1c, but there was a negative correlation between ADMA levels and NO. ADMA and NO may serve as predictors for future cardiovascular events in T2DM patients. Further studies are required to establish the utility of decreasing ADMA levels or increasing NO in the management of T2DM patients.
{"title":"Role of Asymmetric Dimethylarginine and Nitric Oxide in Type 2 Diabetes Mellitus and Its Cardiovascular Complications","authors":"M. Alkady, I. Ibrahim","doi":"10.21608/ejrsa.2019.13784.1074","DOIUrl":"https://doi.org/10.21608/ejrsa.2019.13784.1074","url":null,"abstract":"AN ENDOGENOUS inhibitor of nitric oxide synthase, asymmetric dimethylarginine (ADMA), is elevated in patients with type 2 diabetes mellitus (T2DM) and has been related to atherosclerotic disease. The study aims at investigating the status of ADMA and nitric oxide (NO) and their possible correlation inpatients with T2DM with and without cardiovascular complicationsin comparison to normalcontrols. Forty patients with T2DM and 20 age, sex and body mass index (BMI)‑matched healthy controls were included in the study. The studied individuals were divided into Group1: healthy controls (n= 20), Group 2: T2DM patients without cardiovascular complications (n= 20) and Group 3: T2DM patients with evidence of cardiovascular complications (n= 20). Serum ADMA levels were determined by enzyme linked Immunosorbent Assay (ELISA). Serum nitric oxide was measured as stable end product, nitrite. Insulin was measured by immunoradiometric method. Serum ADMA levels showed a significant elevation while serum NO levels were significantly reducedin diabetic patients groups in comparison to controls. Diabetic patients with vascular complications showed highly significant increase in ADMA levels and pronounced decrease in NO compared to those without complications. In the group of vascular complications, the ADMA level was positively correlated with postprandial serum glucose and HbA1c, but there was a negative correlation between ADMA levels and NO. ADMA and NO may serve as predictors for future cardiovascular events in T2DM patients. Further studies are required to establish the utility of decreasing ADMA levels or increasing NO in the management of T2DM patients.","PeriodicalId":11658,"journal":{"name":"Egyptian Journal of Radiation Sciences and Applications","volume":"56 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88968978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-10-21DOI: 10.21608/ejrsa.2019.15164.1079
H. Youssef, H. Salem
REACTIVE oxygen species (ROS) are generated in response to endogenous or exogenous stimuli. Antioxidants defense system resists for balancing ROS-mediated injury; if oxidation exceeds the defense mechanisms, oxidative stress is generated. Oxidative stress may be involved in the development of breast cancer. Moreover, radiationtherapy (RT), used for the treatment of breast cancer, works by the production of reactive oxygen species at the site of radiation which leads to local oxidative stress. Studies which detect one or few oxidant and antioxidant markers failed to detect the overall oxidant/antioxidant status of the subjects. The authors aim at studying the impact of radiotherapy on the total oxidant status (TOS), total antioxidant status (TAS) with calculation of oxidative stress index (OSI), and measure the lipid peroxidation (MDA) in breast cancer patients.TAS, TOS, MDA and OSI in healthy and breast cancer groups are measured. In breast cancer group, all parameters were measuredbefore and after radiation therapy.In the breast cancer group, TOS, OSI and MDA levels have increased significantly (P<0.001) and the TAS level has decreased (P<0.001) in the breast cancer patients after radiotherapy than before radiotherapy. Breast cancer group TAS after RT reaches about forth its level measured inthe control group. Radiotherapy in breast cancer patients depletes the total antioxidants (TAS), increases total oxidative status (TOS), lipid peroxidation (MDA) and OSI. Breast cancer and its treatment modalities display the patients in a state of severe oxidative stress which requires the supplementation of antioxidants.
{"title":"Oxidative Stress Parameters in Patients with Breast Cancer before and after Radiotherapy","authors":"H. Youssef, H. Salem","doi":"10.21608/ejrsa.2019.15164.1079","DOIUrl":"https://doi.org/10.21608/ejrsa.2019.15164.1079","url":null,"abstract":"REACTIVE oxygen species (ROS) are generated in response to endogenous or exogenous stimuli. Antioxidants defense system resists for balancing ROS-mediated injury; if oxidation exceeds the defense mechanisms, oxidative stress is generated. Oxidative stress may be involved in the development of breast cancer. Moreover, radiationtherapy (RT), used for the treatment of breast cancer, works by the production of reactive oxygen species at the site of radiation which leads to local oxidative stress. Studies which detect one or few oxidant and antioxidant markers failed to detect the overall oxidant/antioxidant status of the subjects. The authors aim at studying the impact of radiotherapy on the total oxidant status (TOS), total antioxidant status (TAS) with calculation of oxidative stress index (OSI), and measure the lipid peroxidation (MDA) in breast cancer patients.TAS, TOS, MDA and OSI in healthy and breast cancer groups are measured. In breast cancer group, all parameters were measuredbefore and after radiation therapy.In the breast cancer group, TOS, OSI and MDA levels have increased significantly (P<0.001) and the TAS level has decreased (P<0.001) in the breast cancer patients after radiotherapy than before radiotherapy. Breast cancer group TAS after RT reaches about forth its level measured inthe control group. Radiotherapy in breast cancer patients depletes the total antioxidants (TAS), increases total oxidative status (TOS), lipid peroxidation (MDA) and OSI. Breast cancer and its treatment modalities display the patients in a state of severe oxidative stress which requires the supplementation of antioxidants.","PeriodicalId":11658,"journal":{"name":"Egyptian Journal of Radiation Sciences and Applications","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73502784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-09-04DOI: 10.21608/ejrsa.2019.12994.1071
Dina Elessawi, Aymen Nasf, A. Shalash, D. Husseiny, Ebthal Bakry
Ischemia is the main cause of stroke, typically due to the occlusion of a cerebral artery as a result of progressive atherosclerosis or an embolus from the heart or neck vessels (Truelsen et al., 2006). In some patients the blockage or occlusion can develop within small intracranial vessels, often because of uncontrolled hypertension or diabetes (Adams et al., 1993). Irrespective of the cause or mechanism of ischemia, collateral flow “i.e, perfusion via alternative or indirect pathways” might off set potential injury to the brain (Liebeskind, 2007).
缺血是中风的主要原因，通常是由于动脉粥样硬化进展或心脏或颈部血管栓塞导致脑动脉闭塞(Truelsen et al.， 2006)。在一些患者中，阻塞或闭塞可发生在颅内小血管内，通常是由于未控制的高血压或糖尿病(Adams et al.， 1993)。无论缺血的原因或机制如何，侧支血流都可以引起缺血。e，通过替代或间接途径灌注”可能会消除对大脑的潜在损伤(Liebeskind, 2007)。
{"title":"Assessment of Cerebral Collaterals in Acute Ischemic Stroke by CT Cerebral Angiography and Its Relation to the Functional Outcome","authors":"Dina Elessawi, Aymen Nasf, A. Shalash, D. Husseiny, Ebthal Bakry","doi":"10.21608/ejrsa.2019.12994.1071","DOIUrl":"https://doi.org/10.21608/ejrsa.2019.12994.1071","url":null,"abstract":"Ischemia is the main cause of stroke, typically due to the occlusion of a cerebral artery as a result of progressive atherosclerosis or an embolus from the heart or neck vessels (Truelsen et al., 2006). In some patients the blockage or occlusion can develop within small intracranial vessels, often because of uncontrolled hypertension or diabetes (Adams et al., 1993). Irrespective of the cause or mechanism of ischemia, collateral flow “i.e, perfusion via alternative or indirect pathways” might off set potential injury to the brain (Liebeskind, 2007).","PeriodicalId":11658,"journal":{"name":"Egyptian Journal of Radiation Sciences and Applications","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90820779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-22DOI: 10.21608/EJRSA.2019.12489.1069
H. El-Said, S. El-Deen, M. Aydia, M. El-Naggar, K. M. El-Azony
NEUTRON activation of tin metal and cobalt chloride targets for production of 113Sn and 125Sb and 60Co was carried out in Egypt’s Second Research Reactor (ETRR-2). The 113Sn, 125Sb and 60Co were loaded onto zirconium silicotungestate gel matrix from aqueous solution at pH 1 by the batch technique. The results obtained during the adsorption process of radionuclides were explained through the Visual MINTEQ program version 3. Then 1 and 0.1g of 113Sn. 125Sb and 60Co-ZrSiW were packed into the volume and planar sealed sources, made of Chinese Artelon, respectively. The radioactivity levels of 113Sn, 125Sb and 60Co were measured and found to be (2.96±0.26, 2.22±0.26, 0.74±0.074kBq) and (0.3±0.03, 026±0.03, 0.11±0.01kBq) for the volume and planar sealed source, respectively, on 27 June 2018. The efficiency curves for the two sealed sources were determined in the energy range 36-1332.5keV, which were measured at 5cm from the HPGe-detector to determine the radioactivity levels of unknown samples for the neutron activation method.
{"title":"Preparation and Quality Control on 113Sn, 125Sb and 60Co Adsorbed on ZrSiW: Volume and Planar Reference Source for -Detector Calibration","authors":"H. El-Said, S. El-Deen, M. Aydia, M. El-Naggar, K. M. El-Azony","doi":"10.21608/EJRSA.2019.12489.1069","DOIUrl":"https://doi.org/10.21608/EJRSA.2019.12489.1069","url":null,"abstract":"NEUTRON activation of tin metal and cobalt chloride targets for production of 113Sn and 125Sb and 60Co was carried out in Egypt’s Second Research Reactor (ETRR-2). The 113Sn, 125Sb and 60Co were loaded onto zirconium silicotungestate gel matrix from aqueous solution at pH 1 by the batch technique. The results obtained during the adsorption process of radionuclides were explained through the Visual MINTEQ program version 3. Then 1 and 0.1g of 113Sn. 125Sb and 60Co-ZrSiW were packed into the volume and planar sealed sources, made of Chinese Artelon, respectively. The radioactivity levels of 113Sn, 125Sb and 60Co were measured and found to be (2.96±0.26, 2.22±0.26, 0.74±0.074kBq) and (0.3±0.03, 026±0.03, 0.11±0.01kBq) for the volume and planar sealed source, respectively, on 27 June 2018. The efficiency curves for the two sealed sources were determined in the energy range 36-1332.5keV, which were measured at 5cm from the HPGe-detector to determine the radioactivity levels of unknown samples for the neutron activation method.","PeriodicalId":11658,"journal":{"name":"Egyptian Journal of Radiation Sciences and Applications","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75253996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-07DOI: 10.21608/EJRSA.2019.14648.1077
S. Montaser, Mahmoudi Mahmoud, S. Ibrahim
THE POSSIBLE antioxidant role of Achillea millefolium L. (ACM) extract was investigated against mutagenecity and immunological disorders in irradiated human blood cultures. Peripheral blood samples were collected from healthy human volunteers then incubated in the prepared cultures. The whole blood was exposed in vitro to 3 Gy of γ-ray and then the blood was cultured with mitogenic stimulation and ACM extract at different concentrations (100 & 200μg/ml). After 72hr of incubation, cytokinesis-block micronucleus analysis (CBMN) and immunological parameters: Interleukin-8 (IL-8), interleukin-18 (IL-18), cyclooxygenase-2 (COX-2) and tumor necrosis factor (TNF-α) were investigated. At each dose point, the treatment of the blood with ACM showed a significant decrease in the incidences of micronuclei (one, two and three micronuclei). Also, there are amelioration in the levels of IL-8, IL-18, Cox-2 and TNF-α. The maximum protection effect according to the selected parameters was observed at 200μg/ml of ACM extract. The presented data suggested that ACM may play important roles as antimutagenic and antinflammatory protectors against radiation exposure.
{"title":"Antioxidant Capacity of Achillea millefolium L against Cytogenetic and Immunological Disorders in Irradiated Human Blood Cultures.","authors":"S. Montaser, Mahmoudi Mahmoud, S. Ibrahim","doi":"10.21608/EJRSA.2019.14648.1077","DOIUrl":"https://doi.org/10.21608/EJRSA.2019.14648.1077","url":null,"abstract":"THE POSSIBLE antioxidant role of Achillea millefolium L. (ACM) extract was investigated against mutagenecity and immunological disorders in irradiated human blood cultures. Peripheral blood samples were collected from healthy human volunteers then incubated in the prepared cultures. The whole blood was exposed in vitro to 3 Gy of γ-ray and then the blood was cultured with mitogenic stimulation and ACM extract at different concentrations (100 & 200μg/ml). After 72hr of incubation, cytokinesis-block micronucleus analysis (CBMN) and immunological parameters: Interleukin-8 (IL-8), interleukin-18 (IL-18), cyclooxygenase-2 (COX-2) and tumor necrosis factor (TNF-α) were investigated. At each dose point, the treatment of the blood with ACM showed a significant decrease in the incidences of micronuclei (one, two and three micronuclei). Also, there are amelioration in the levels of IL-8, IL-18, Cox-2 and TNF-α. The maximum protection effect according to the selected parameters was observed at 200μg/ml of ACM extract. The presented data suggested that ACM may play important roles as antimutagenic and antinflammatory protectors against radiation exposure.","PeriodicalId":11658,"journal":{"name":"Egyptian Journal of Radiation Sciences and Applications","volume":"34 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78743140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-01DOI: 10.21608/EJRSA.2019.5325.1053
L. Bamidele, Osahon. O. David
This study aims at determining radiation doses for patients undergoing six (6) common radio graphic examinations in eight X – ray units in the southern part of Nigeria using computational method. Both public and private – owned hospitals were used for the study. Patient data and technical parameters were collected during the examinations. The meanentrance surface air kerma (ESAK) obtained from all the x-rays units considered ranged from 0.35 to 1.96mGy for Chest PA, 1.01 to 17.55mGy for Abdomen PA; 1.08 to 16.14mGy for pelvis AP,1.12 to 10.06mGy for Pelvis LAT, 0.08 to 3.54mGy for Skull AP/PA; 1.07 to 4.61 for Skull LAT, 1.67 to 12.46mGy for Lumber Spine AP, 1.82 to 14.24mGy for Lumber Spine LAT. Local diagnostic reference levels (LDRLs) were established based on the third quartile of ESAKs values. The established LDRLs were compared with previously established DRLs from other countries. The ESAKs values obtained in this study were found to be within the international established DRLs except in few cases.
{"title":"Dose Audits of Patients Undergoing Some Common Radiographic Examinations in Selected Nigerian Hospitals","authors":"L. Bamidele, Osahon. O. David","doi":"10.21608/EJRSA.2019.5325.1053","DOIUrl":"https://doi.org/10.21608/EJRSA.2019.5325.1053","url":null,"abstract":"This study aims at determining radiation doses for patients undergoing six (6) common radio graphic examinations in eight X – ray units in the southern part of Nigeria using computational method. Both public and private – owned hospitals were used for the study. Patient data and technical parameters were collected during the examinations. The meanentrance surface air kerma (ESAK) obtained from all the x-rays units considered ranged from 0.35 to 1.96mGy for Chest PA, 1.01 to 17.55mGy for Abdomen PA; 1.08 to 16.14mGy for pelvis AP,1.12 to 10.06mGy for Pelvis LAT, 0.08 to 3.54mGy for Skull AP/PA; 1.07 to 4.61 for Skull LAT, 1.67 to 12.46mGy for Lumber Spine AP, 1.82 to 14.24mGy for Lumber Spine LAT. Local diagnostic reference levels (LDRLs) were established based on the third quartile of ESAKs values. The established LDRLs were compared with previously established DRLs from other countries. The ESAKs values obtained in this study were found to be within the international established DRLs except in few cases.","PeriodicalId":11658,"journal":{"name":"Egyptian Journal of Radiation Sciences and Applications","volume":"43 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81127745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-01DOI: 10.21608/EJRSA.2019.7166.1062
I. Salama, M. K. A. el-Megid
THE AIM of this study is to determine the oleandrin concentrations in the Nerium oleander cell suspension culture after being exposed to UV-A (365nm), at different time intervals (0 controls, 5, 10, 15, 20, 25, 30, 35, 40, 45 and 50min). The results showed that the maximum time limits for carrying N. oleander cells suspension culture to exposure of UV-A is 30min.The present study also aims at investigating changes of the protein bands, peroxidase isozyme and oleandrin concentration at the same condition. Oleandrin was separated and quantified by HPLC. The investigation of protein changes was conducted using SDS PAGE electrophoresis techniques. The results revealed that the changes of the protein bands depend on different time intervals of exposure to A-UV radiation. Thus, the changes of the oleandrin production were consequent to gene expression changes. Peroxidase isozyme analysis, showed that exposure to UV-A for the 5, 15 and 20min had a clear impact on the peroxidase isozyme, but the greatest impact was in the sample exposed to UV-A for a period of 15 minutes (0.093±0.01) μg/ml, the same sample showed the highest concentration of oleandrin compared to the control (0.022±0.001) μg/ml. However, the other samples were less affected by the exposure to UV-A.
{"title":"Reflex of oleandrin production and molecular changes on the Nerium oleander L. cell suspension culture under UV-A radiation stress effect.","authors":"I. Salama, M. K. A. el-Megid","doi":"10.21608/EJRSA.2019.7166.1062","DOIUrl":"https://doi.org/10.21608/EJRSA.2019.7166.1062","url":null,"abstract":"THE AIM of this study is to determine the oleandrin concentrations in the Nerium oleander cell suspension culture after being exposed to UV-A (365nm), at different time intervals (0 controls, 5, 10, 15, 20, 25, 30, 35, 40, 45 and 50min). The results showed that the maximum time limits for carrying N. oleander cells suspension culture to exposure of UV-A is 30min.The present study also aims at investigating changes of the protein bands, peroxidase isozyme and oleandrin concentration at the same condition. Oleandrin was separated and quantified by HPLC. The investigation of protein changes was conducted using SDS PAGE electrophoresis techniques. The results revealed that the changes of the protein bands depend on different time intervals of exposure to A-UV radiation. Thus, the changes of the oleandrin production were consequent to gene expression changes. Peroxidase isozyme analysis, showed that exposure to UV-A for the 5, 15 and 20min had a clear impact on the peroxidase isozyme, but the greatest impact was in the sample exposed to UV-A for a period of 15 minutes (0.093±0.01) μg/ml, the same sample showed the highest concentration of oleandrin compared to the control (0.022±0.001) μg/ml. However, the other samples were less affected by the exposure to UV-A.","PeriodicalId":11658,"journal":{"name":"Egyptian Journal of Radiation Sciences and Applications","volume":"2004 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78866031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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