Mu-Han Yang, Sanaz Sadegh, P. Saisan, Christopher G. L. Ferri, A. Devor, Y. Fainman
In non-degenerate 2-photon excitation (ND-2PE) microscopy, a fluorophore simultaneously absorbs two photons of different energies. We performed a ND-2PE study of fluorescent proteins and synthetic dyes (eg.eGFP, FITC, and etc.) continuously varying energies and numbers of both photons to create two-dimensional map of fluorescence landscapes. By using the best photon energy combination from our two-dimensional map, we found an increase in detected fluorescent image brightness with ND-2PE as we imaged cortical neurons labeled with enhanced green fluorescent protein (eGFP). It should be noted that the photons corresponding to longer wavelength will penetrate deeper into the tissue at reduced scattering. Additionally, using non-overlapping spatial modes carrying the photons at different energies will significantly reduce out of focus fluorescence from the large number of low energy photons, and by a proper choice of the number of high energy photons the ND-2PE fluorescence can be obtained from deep tissue. Experimentally, we strategically displaced two laser beams until they reached the sample plane such that the unwanted background in the excitation beam path was suppressed. In contrast, these two pump beams were well overlapped at focus which still produced sufficient number fluorescence photons for detection. In our experiment, the temporal alignment was achieved with optical delay line in the optical path of IR beam. With this technique we demonstrated experimentally that ND-2PE with side-by-side beams provided a better signal to background ratio in the scattering phantom as compared with D-2PE. The excitation volume of ND-2PE with side-by-side beam was also investigated and determined to be comparable in size with that of the D-2PE.
{"title":"Deep image with non-degenerated two-photon excitation (Conference Presentation)","authors":"Mu-Han Yang, Sanaz Sadegh, P. Saisan, Christopher G. L. Ferri, A. Devor, Y. Fainman","doi":"10.1117/12.2320384","DOIUrl":"https://doi.org/10.1117/12.2320384","url":null,"abstract":"In non-degenerate 2-photon excitation (ND-2PE) microscopy, a fluorophore simultaneously absorbs two photons of different energies. We performed a ND-2PE study of fluorescent proteins and synthetic dyes (eg.eGFP, FITC, and etc.) continuously varying energies and numbers of both photons to create two-dimensional map of fluorescence landscapes. By using the best photon energy combination from our two-dimensional map, we found an increase in detected fluorescent image brightness with ND-2PE as we imaged cortical neurons labeled with enhanced green fluorescent protein (eGFP). It should be noted that the photons corresponding to longer wavelength will penetrate deeper into the tissue at reduced scattering. Additionally, using non-overlapping spatial modes carrying the photons at different energies will significantly reduce out of focus fluorescence from the large number of low energy photons, and by a proper choice of the number of high energy photons the ND-2PE fluorescence can be obtained from deep tissue. Experimentally, we strategically displaced two laser beams until they reached the sample plane such that the unwanted background in the excitation beam path was suppressed. In contrast, these two pump beams were well overlapped at focus which still produced sufficient number fluorescence photons for detection. In our experiment, the temporal alignment was achieved with optical delay line in the optical path of IR beam. With this technique we demonstrated experimentally that ND-2PE with side-by-side beams provided a better signal to background ratio in the scattering phantom as compared with D-2PE. The excitation volume of ND-2PE with side-by-side beam was also investigated and determined to be comparable in size with that of the D-2PE.","PeriodicalId":117167,"journal":{"name":"Ultrafast Nonlinear Imaging and Spectroscopy VI","volume":"252 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134058701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Coherent Raman scattering at interfaces (Conference Presentation)","authors":"E. Potma, John P Kenison","doi":"10.1117/12.2322636","DOIUrl":"https://doi.org/10.1117/12.2322636","url":null,"abstract":"","PeriodicalId":117167,"journal":{"name":"Ultrafast Nonlinear Imaging and Spectroscopy VI","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125288073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Erkang Wang, A. Chicco, R. Bartels, Jesse W. Wilson
{"title":"Picosecond dynamics in hemes for mitochondrial functional contrast (Conference Presentation)","authors":"Erkang Wang, A. Chicco, R. Bartels, Jesse W. Wilson","doi":"10.1117/12.2320326","DOIUrl":"https://doi.org/10.1117/12.2320326","url":null,"abstract":"","PeriodicalId":117167,"journal":{"name":"Ultrafast Nonlinear Imaging and Spectroscopy VI","volume":"36 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131176012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Interpretation of the vibrational sum frequency spectroscopy (Conference Presentation)","authors":"Zhou Lu","doi":"10.1117/12.2320091","DOIUrl":"https://doi.org/10.1117/12.2320091","url":null,"abstract":"","PeriodicalId":117167,"journal":{"name":"Ultrafast Nonlinear Imaging and Spectroscopy VI","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115894749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
C. Moser, P. Delrot, D. Loterie, Georgia Konstantinou, D. Psaltis
We show that a multimode fiber can be used to deliver shaped light, either continuous wave or ultra fast pulses, in a resin in order to build useful complex three dimensional objects in areas difficult or impossible to reach with conventional manufacturing tools. We show complex objects that are made by additive manufacturing with either a single photon or a 2 photon process. We investigate the build volume and resolution that are possible by engineering the fiber tip.
{"title":"3D printing with multimode fibers (Conference Presentation)","authors":"C. Moser, P. Delrot, D. Loterie, Georgia Konstantinou, D. Psaltis","doi":"10.1117/12.2322517","DOIUrl":"https://doi.org/10.1117/12.2322517","url":null,"abstract":"We show that a multimode fiber can be used to deliver shaped light, either continuous wave or ultra fast pulses, in a resin in order to build useful complex three dimensional objects in areas difficult or impossible to reach with conventional manufacturing tools. We show complex objects that are made by additive manufacturing with either a single photon or a 2 photon process. We investigate the build volume and resolution that are possible by engineering the fiber tip.","PeriodicalId":117167,"journal":{"name":"Ultrafast Nonlinear Imaging and Spectroscopy VI","volume":"214 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114205143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Picosecond ultrasonics and transient thermoreflectance methods for characterization of grain boundary microstructure and thermal transport (Conference Presentation)","authors":"M. Khafizov, D. Hurley","doi":"10.1117/12.2321902","DOIUrl":"https://doi.org/10.1117/12.2321902","url":null,"abstract":"","PeriodicalId":117167,"journal":{"name":"Ultrafast Nonlinear Imaging and Spectroscopy VI","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125037876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Generation of few-cycle laser pulses from the nonlinear fiber system (Conference Presentation)","authors":"Ming Hu","doi":"10.1117/12.2320409","DOIUrl":"https://doi.org/10.1117/12.2320409","url":null,"abstract":"","PeriodicalId":117167,"journal":{"name":"Ultrafast Nonlinear Imaging and Spectroscopy VI","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114994635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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