Frontiers in Natural Product Chemistry最新文献

{"title":"Molecular cloning of cellulase genes from Trichoderma harzianum.","authors":"Sibtain Ahmed, N. Aslam, F. Latif, M. Rajoka, A. Jamil","doi":"10.2174/1574089054583669","DOIUrl":"https://doi.org/10.2174/1574089054583669","url":null,"abstract":"Cellulases are attractive source for utilization of the agro-industrial waste materials. Exoglucanase (EC; 3.2.1.91), Endoglucanase (EC. 3.2.1.4) and βglucosidase (EC. 3.2.1.21) were isolated from Trichoderma harzianum (E-58 strain). The fungus was grown on Vogel's medium with different carbon sources. Maximal production of the enzymes was achieved at 28 C, pH 5.5 under continuous shaking at 120 rpm for 5 days. Glucose repressed the synthesis of the enzymes whereas carboxymethylcellulose (CMC) produced the enzymes in substantial amounts. Maximum activity of cellulases (exoglucanase, endoglucanase and β-glucosidase) were found to be 2.764, 14.4 and 0.629IU mL, respectively. Corresponding genes for cellulases were isolated with the help of RTPCR. RNA was isolated from mycelia of T. harzianum grown on CMC and xylan. First strand of cDNA was synthesized using oligo dT (18) primer and subjected to PCR with specific primers. The amplified products were purified through agarose gel electrophoresis and ligated into SmaI site of pUC18. The plasmids containing exg, egl and bgl genes were transformed into E. coli for further characterization. INTRODUCTION Despite the fact that the world community is no longer pre-occupied with fossil fuel shortage, there is still a considerable research and development directed towards understanding and commercializing enzymatic hydrolysis of cellulose [1]. Cellulose is the most abundant organic polymer in this planet and is an important renewable energy source along with sugars and starches [2]. Energy production from cellulosic raw material involves its hydrolysis into glucose. Highly ordered cellulose substrates are converted into soluble sugars only when exoglucanase, endoglucanase and β-glucosidase are present in solution simultaneously in right proportion. A number of fungal species are known for the production of cellulases such as Aspergillus niger, Sporotrichum spp, Chaetomium thermophile, Trichoderma species etc [3]. Gene cloning is recently being employed for studying the structure and function of a number of enzymes and proteins and their over expression. This strategy has been found very efficient as compared to other traditional methods. Present study was designed to layout strategy for enhanced production of cellulases. In this paper we have reported the isolation and cloning of cellulase genes (exg ,egl and bgl) from T.harzianum (E-58 strain). MATERIALS AND METHODS Growth Conditions and Enzyme Assay Trichoderma harzianum (E58) was grown at 28C with shaking at 120 rpm in Vogel's medium (0.5% Trisodium citrate, 0.5% KH2PO4.0.2% NH4NO3, 0.4% (NH4)2SO4, 0.02%","PeriodicalId":117391,"journal":{"name":"Frontiers in Natural Product Chemistry","volume":"38 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117031806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mistletoe lectins, structure and function.","authors":"W. Voelter, R. Wacker, S. Stoeva, R. Tsitsilonis, C. Betzel, Atta-ur- Rahman, M. Choudhary, K. Khan","doi":"10.2174/1574089054583597","DOIUrl":"https://doi.org/10.2174/1574089054583597","url":null,"abstract":"","PeriodicalId":117391,"journal":{"name":"Frontiers in Natural Product Chemistry","volume":"53 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133106497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tandem Stereospecific Radical Cation-Mediated Syntheses of Oligostilbenoid Dimers","authors":"N. F. Thomas, K. C. Lee, J. Weber, I. Wahab, K. Awang, A. Hadi, P. Richomme","doi":"10.2174/1574089054583768","DOIUrl":"https://doi.org/10.2174/1574089054583768","url":null,"abstract":"","PeriodicalId":117391,"journal":{"name":"Frontiers in Natural Product Chemistry","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116995873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Impacts of Covid-19 on Household Behavior and Household Waste in Turkey","authors":"S. Yalçınyigit, A. Dönmez-Turan, H. Akbaş, G. Varank","doi":"10.13135/2384-8677/6258","DOIUrl":"https://doi.org/10.13135/2384-8677/6258","url":null,"abstract":"COVID-19 has caused many radical changes in our daily life. The main purpose of this study is to examine the impact of the pandemic on household behavior and waste changes in the largest city of Turkey, Istanbul. Research data were collected from 946 participants in the period May to December 2020 through an online survey, and frequency and logistic regression analyses were conducted. Frequency analysis of the data showed that mask, glove, disposable bag usage, cooking at home, online shopping, home delivery, dishwasher and washing machine usage increased, while ordering food from outside decreased. In line with these behavioral changes, we found that medical, food, disposable bag and plastic wastes generated by the sampled households increased as well. We then conducted logistic regression analysis to examine the correlation between changes in household behavior and waste disposal and the income and size of households during this pandemic period. The empirical findings highlight the importance of developing special management strategies for medical, food and plastic wastes and for households with different socio-economic backgrounds to reduce the unsustainable environmental impact of COVID-19. © 2022, Bentham Science Publishers. All rights reserved.","PeriodicalId":117391,"journal":{"name":"Frontiers in Natural Product Chemistry","volume":"186 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114837442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical investigation on Zingiber zerumbet Sm.","authors":"M. Hossain, S. Bhattacharjee, M. Islam","doi":"10.2174/1574089054583722","DOIUrl":"https://doi.org/10.2174/1574089054583722","url":null,"abstract":"","PeriodicalId":117391,"journal":{"name":"Frontiers in Natural Product Chemistry","volume":"61 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128107741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of absolute configuration of natural products by X-ray diffraction: a novel approach of incorporating heavy-atom-containing solvent molecules into the single crystals and refinement of flack parameter.","authors":"S. Chantrapromma, Fun HoongKun, S. Laphookhieo, S. Cheenpracha, C. Karalai, Atta-ur- Rahman, M. Choudhary, K. Khan","doi":"10.2174/1574089054583759","DOIUrl":"https://doi.org/10.2174/1574089054583759","url":null,"abstract":"","PeriodicalId":117391,"journal":{"name":"Frontiers in Natural Product Chemistry","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133691581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial Transformation of Natural Products- A Tool for the Synthesis of Novel Analogues of Bioactive Substances","authors":"M. Choudhary, S. Musharraf","doi":"10.2174/1574089054583830","DOIUrl":"https://doi.org/10.2174/1574089054583830","url":null,"abstract":"","PeriodicalId":117391,"journal":{"name":"Frontiers in Natural Product Chemistry","volume":"60 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133353662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structure and Biological Activity - Diversity Orientated Synthesis","authors":"E. Winterfeldt","doi":"10.2174/1574089054583795","DOIUrl":"https://doi.org/10.2174/1574089054583795","url":null,"abstract":"","PeriodicalId":117391,"journal":{"name":"Frontiers in Natural Product Chemistry","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127621675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Researches on the Technology and Bioactive Properties of Phenolic Lipids","authors":"J. Tyman","doi":"10.2174/1574089054583605","DOIUrl":"https://doi.org/10.2174/1574089054583605","url":null,"abstract":"","PeriodicalId":117391,"journal":{"name":"Frontiers in Natural Product Chemistry","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130702805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cyclitols: Conduritols Aminoconduritols and Quercitols","authors":"M. Balci, Murat Çelik, Emine Demir, M. Ertaş, Serdar Gultekin, Nihal Ozturk, Yunus Kara, Nurhan Horasan-Kishali","doi":"10.2174/1574089054583696","DOIUrl":"https://doi.org/10.2174/1574089054583696","url":null,"abstract":"","PeriodicalId":117391,"journal":{"name":"Frontiers in Natural Product Chemistry","volume":"119 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131007667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}