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Proximate Composition and Amino Acids Contents in Selected Legumes and Hura crepitans seed in Wukari Taraba State 乌卡里塔拉巴州部分豆科植物和胡拉种子的近似组成和氨基酸含量
Pub Date : 2020-06-30 DOI: 10.47231/sfxi7117
K. Arowora, C. Popoola, O. Yakubu, Kenneth Chinekwu Ugwuoke, F. Aliyu
: The study evaluated the proximate composition and amino acid contents of selected legumes and Hura crepitans seed in Wukari,Taraba State, Nigeria. The Sand box seeds were collected from its tree in Wukari town, while black eyed pea and red kidney beans were purchased from Wukari market. The samples were processed into powder for proximate composition and amino profile. In all the samples, 18 amino acids were determined with higher values for aspartic acid, glutamic acid, leucine and phenylalanine while cysteine (0.43-0.79g/ 100g) had the lowest value in the samples analysed. Levels of amino acid ranged as: Sandbox seed had 0.79 ± 0.02 to 10.44 ± 0.02 g/100g, Black eyed pea seed had 0.51 ± 0.12 to 8.53 ± 0.03 g/100g, red kidney bean had 0.43 ± 0.02 to 7.93 ± 0.02 g/100g. The sand box seed had the highest protein content (33.71± 0.02 %), followed by red kidney bean (29.21 ± 0.02%). Nitrogen free extract had the highest value in black eyed pea (63.29±0.01%). The sand box seed had the highest crude fat (7.27 ± 0.02%). All the three crop samples had low safe moisture levels for storage. The study revealed the variation in the nutrients composition of the crop samples. However, sand box seeds had the highest protein content, coupled with high levels of amino acids detected, but the seed is yet to be consumed by humans. Hence, the need for further research on Hura crepitans with the view of reducing the deleterious effects of its antinutrients.
:本研究评估了尼日利亚塔拉巴州乌卡里选定的豆科植物和胡拉藜种子的近似组成和氨基酸含量。沙盒种子是从乌卡里镇的树上采集的,而黑眼豆和红芸豆则是从乌卡里市场购买的。样品被加工成粉末,以获得近似的成分和氨基分布。在所有样品中,有18种氨基酸的天冬氨酸、谷氨酸、亮氨酸和苯丙氨酸的测定值较高,而半胱氨酸(0.43-0.79g/ 100g)的测定值最低。氨基酸含量的变化范围为:沙箱籽为0.79±0.02 ~ 10.44±0.02 g/100g,黑眼豌豆籽为0.51±0.12 ~ 8.53±0.03 g/100g,红芸豆为0.43±0.02 ~ 7.93±0.02 g/100g。砂箱种子蛋白质含量最高(33.71±0.02%),其次为红芸豆(29.21±0.02%)。无氮提取物在黑眼豆中的含量最高(63.29±0.01%)。砂箱种子粗脂肪含量最高(7.27±0.02%)。这三种作物样本的安全湿度都很低。这项研究揭示了作物样品中营养成分的变化。然而,沙盒种子的蛋白质含量最高,而且检测到的氨基酸含量也很高,但这种种子还没有被人类食用。因此,有必要进一步研究胡拉草,以减少其抗营养物质的有害作用。
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引用次数: 0
Haematological and Hepatological effects of Pito, Burukutu-Guinea corn and Burukutu-Millet in Albino Rats 皮托、布鲁库图-几内亚玉米和布鲁库图-谷子对白化大鼠血液学和肝脏学的影响
Pub Date : 2020-06-30 DOI: 10.47231/irrt7781
C. Imo, Boy Richard-Harris, Bassey Victor Nsan, Emmanuel Williams Adamu
: This study investigated the haematological and hepatological effects of selected locally brewed beers in albino rats. Three locally brewed beers were purchased from locally brewed beer joint in Wukari, Nigeria. Twenty male albino rats were used and were randomly distributed into four groups of five rats each. Group 1 served as normal control, while groups 2, 3 and 4 were administered pito, burukutu-Guinea corn and burukutu-millet (10mL/kg bwt) respectively for 21 days before being sacrificed. Result showed that ALT and AST activities increased in all the groups administered the different beer compared to the control. Cholesterol increased in group 2, but decreased in group 4. Potassium and glucose reduced in all test groups compared to the normal control. The differences in cholesterol, potassium and glucose levels are statistically less-significant (p>0.05) compared to the control. The liver histoarchitectural state of the test animals administered the beers compared to the control animals showed the beers had toxic effect on the animals. There is evidence of infiltration and distortion of some regions of the liver tissue in the test animals. This agrees with the results of serum biochemical parameters evaluated. The WBC, LYM and GRA increased in group 3 and reduced in groups 2 and 4 compared to the control. The MID reduced in all the test groups compared to the control. RBC, Hb and HCT increased in all the test groups compared to the control. groups 2 and 3. The MCH, MCHC and RDWc reduced in all the test groups, while PLT, PCT, MPV and PDWc increased in all the test groups compared to the control. This study has shown that constant administration of pito, burukutu-guinea corn and burukutu-millet in the test animals for a long duration caused alterations and negative effect on the liver function, but may not interfere negatively with the Hb and RBC count.
本研究考察了当地酿造啤酒对白化大鼠血液学和肝脏学的影响。三种当地酿造的啤酒是从尼日利亚乌卡里当地酿造的啤酒店购买的。选取雄性白化大鼠20只,随机分为4组,每组5只。第1组为正常对照组,第2、3、4组分别给予皮托、布鲁库图-几内亚玉米和布鲁库图-谷子(10mL/kg bwt) 21 d后处死。结果表明,与对照组相比,各啤酒组的谷丙转氨酶和谷丙转氨酶活性均有所提高。2组胆固醇升高,4组胆固醇降低。与正常对照组相比,所有实验组的钾和葡萄糖含量都有所降低。与对照组相比,胆固醇、钾和葡萄糖水平差异无统计学意义(p>0.05)。与对照组相比,喝了啤酒的实验动物的肝脏组织结构状态表明,啤酒对动物有毒性作用。实验动物肝组织的某些区域有浸润和变形的迹象。这与血清生化指标评价结果一致。WBC、LYM和GRA在第3组较对照组升高,第2组和第4组较对照组降低。与对照组相比,所有试验组的MID都降低了。与对照组相比,所有试验组的RBC、Hb和HCT均升高。第二组和第三组。各试验组MCH、MCHC、RDWc均较对照组降低,PLT、PCT、MPV、PDWc均较对照组升高。本研究表明,在实验动物中长期持续服用皮托、布鲁库图-几内亚玉米和布鲁库图-谷子对肝功能产生改变和负面影响,但可能不会对Hb和RBC计数产生负面干扰。
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Covenant Journal of Physical and Life Sciences
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