Expert Review of Clinical Immunology最新文献

Background: Severe asthma is a complex disease with persistent symptoms despite high-dose inhaled therapy. Tezepelumab, a monoclonal antibody targeting thymic stromal lymphopoietin (TSLP), has shown efficacy across asthma phenotypes. However, identifying early responders remains a challenge. Basophils, key players in type 2 inflammation, may serve as predictive biomarkers.

Objective: We evaluated the presence of super-responder status after six months of Tezepelumab therapy and explored the predictive role of blood basophil levels.

Methods: A real-life, prospective study was conducted on 16 severe asthma patients. Super-responders were defined per Upham et al.'s criteria, adapted for a six-month assessment. Clinical, functional, and inflammatory parameters, including blood basophil counts, were analyzed.

Results: After six months, 62.5% of patients achieved super-responder status, with complete exacerbation elimination, reduced oral corticosteroid use, and improved asthma control. A significant logarithmic association (p = 0.019) was found between baseline basophil levels and super-responder status, indicating that higher basophil counts were associated with an increased likelihood of super-response. This finding was supported by a trend toward significance in ROC curve analysis (AUC = 0.800, p = 0.050), suggesting potential predictive value.

Conclusion: Tezepelumab demonstrates early efficacy in severe asthma, and baseline blood basophil levels may represent a promising biomarker for response prediction.

Introduction: The innate immune system's complement system and cGAS-STING pathway play crucial roles in pathogen defense and immune response modulation. Complement's role in cancer is multifaceted, affecting myeloid cells and T cell activities, while intracellular complement (complosome) participates in cellular processes like metabolism and autophagy. STING's activation influences tumor dynamics through NF-κB pathway interactions, enhancing or suppressing tumor responses. The interplay between complement and cGAS-STING pathways suggests potential regulatory crosstalk affecting immune responses, warranting further investigation.

Areas covered: We review the functions of the complement system and the cGAS-STING pathway in pathogen clearance and tumor development. The focus is on exploring the intricate interplay between these pathways and discussing its implications for disease pathogenesis ;(PubMed: 1983-2024).

Expert opinion: Such insights into these pathways could guide therapeutic strategies targeting immune modulation in oncology and autoimmune disorders, emphasizing the need to understand their complex roles in health and disease.

Introduction: About 75% of psoriasis cases in the female population affect women under 40 years of age, hence possibly involving pregnancy or lactation periods. Data concerning common topical and systemic treatments for psoriasis during pregnancy and lactation are limited given the fact that pregnancy is an exclusion criterion for clinical trials.

Areas covered: The aim of our review is to provide an overview of currently available treatments in women with childbearing potential, pregnancy, and breastfeeding, both topical and systemic, including biological therapies and small molecules.

Expert opinion: The choice of the most appropriate treatment must be made on a case-by-case basis, assessing the risk-benefit ratio for the mother and the fetus or offspring. We recommend multidisciplinary management of the patient and consultation of data, if available, from national safety registers on pregnancy outcomes in exposed mothers.

Introduction: Diabetic kidney disease (DKD) is one of the most common causes of chronic kidney disease, leading to end-stage kidney disease (ESKD), and is one of the most significant complications associated with diabetes mellitus. Furthermore, the medical costs of dialysis therapy associated with ESKD are high, and financial strain is a major problem. This review first focuses on the mechanisms of DKD progression and exacerbation, and then describes the 'DKD fantastic four,' which we advocate as the latest DKD treatment.

Areas covered: In DKD, increase in the extracellular matrix of mesangial cells is attributed to transforming growth factor-β/Smad1/type 4 collagen signaling, whose effects are enhanced by angiotensin II signaling.DKD activates protein kinase C (PKC)d, leading to dephosphorylation of vascular endothelial growth factor receptor-2 and reduction of its downstream effects, thereby inducing podocyte apoptosis. PKCβ inhibits insulin receptor substrate 1/Akt/endothelial NO synthase signaling in glomerular endothelial cells.

Expert opinion: Recently, sodium-glucose co-transporter 2 inhibitors have been shown to reduce the risk of progression of nephropathy. Additionally, glucagon-like peptide 1 and glucose-dependent insulinotropic polypeptides elicit vasoactive effects, reducing the risk of DKD. Finerenone, a non-steroidal mineralocorticoid receptor antagonist, reduces the composite renal endpoint without causing severe hyperkalemia.

Introduction: Chronic airway inflammatory diseases mainly comprise chronic rhinosinusitis (CRS), allergic rhinitis (AR), asthma, cystic fibrosis (CF), and chronic obstructive pulmonary disease (COPD). Epithelial cells fulfill a protective role as a barrier; however, when stimulated, these cells also release a variety of cytokines that attract and activate immune cells, including macrophages, neutrophils, and T-lymphocytes. Excessive activation and aggregation of immune cells disrupts the balance of the cellular microenvironment, and leads to impaired immune defense of the airway mucosa, which can further exacerbate an inflammatory response.

Areas covered: In this article, we discuss the key cytokines and immune pathways involved in epithelial-immune cell interactions, and we detail discoveries in the emerging field of single-cell sequencing and summarize monoclonal antibody-targeted therapies. A comprehensive search was conducted using the search terms 'epithelial cell,' 'immune,' 'interaction,' 'cytokines,' 'asthma,' 'chronic sinusitis,' 'allergic rhinitis,' 'monoclonal antibodies,' and 'single-cell sequencing' by querying Google Scholar and PubMed.

Expert opinion: The intricate pathophysiology of airway inflammation remains to be fully elucidated. Emerging technologies, such as single-cell sequencing, have led to a more comprehensive characterization of the immune mechanisms underlying the pathophysiology of airway inflammatory diseases, which points the way to further precision medicine in the future.

Introduction: Metabolic dysfunction-associated steatotic liver disease (MASLD) is the most common liver comorbidity in patients with inflammatory bowel disease (IBD).

Areas covered: This paper outlines common pathophysiological aspects related to MASLD and IBD; describes the epidemiological clues associated with both diseases; analyzes risk factors contributing to MASLD appearance and progression in IBD patients; reviews data on clinical consequences for this population; and provides advice on the management of patients with both conditions.

Expert opinion: IBD itself, especially Crohn's disease, is a risk factor for MASLD and its progression to liver cirrhosis, independent of other cardiometabolic risk factors. Intestine-dependent mechanisms which contribute to MASLD in IBD and interplay with classic metabolic factors include sarcopenia, disease phenotype, duration, and activity. Changes in microbiota also contribute to deregulating the gut-liver axis in these conditions. By contrast, IBD therapies do not seem to play a relevant role in the risk of developing MASLD; and the potential of biologics and novel small molecules on liver changes requires further investigation. MASLD increases comorbidities, impairs clinical outcomes, and increases mortality in IBD patients; therefore, early detection of MASLD is a priority in IBD populations. Individualized and integrative management of both MASLD and IBD is required to improve results.

Introduction: Chronic Obstructive Pulmonary Disease (COPD) is characterized by persistent respiratory symptoms and airflow limitation. A subset of patients exhibits eosinophilic inflammation, which has been shown to impact disease severity and exacerbation frequency.

Areas covered: This review aims to provide an update on the role of eosinophils in COPD and the efficacy of biologics targeting inflammation. Furthermore, we will explore eosinophilia as a biomarker for COPD outcomes. The findings highlight the potential of biologics in managing COPD. We conducted a review of the English-language literature from the beginning of the databases reviewed through April 2025.

Expert opinion: In COPD there is a close interplay between inflammation, lung damage and multimorbidities, mechanisms that determine a lower efficacy of biologics. Patients currently eligible for biologics are only the subpopulation with eosinophilic airway inflammation, but biologics that target broad-acting epithelial cytokines might have a greater efficacy in a complex disease such as COPD. This is because their mechanism of action is not limited to modulation of eosinophilic inflammation alone but to multiple driver pathways. Looking forward, there is an urgent need to identify new biomarkers for better patient selection to improve the impact of biologics, which is still not yet fully satisfactory.

Introduction: Some genetic polymorphisms are associated with the risk of stroke, although the individual contribution of such polymorphisms is considered modest.

Aims: To evaluate the frequency of single nucleotide polymorphisms (SNPs) in genes encoding proinflammatory cytokines and coagulation factors in stroke patients, the relationships between the serum levels of the cytokines analyzed with stroke subtypes and prognosis.

Material and methods: A retrospective, cross-sectional, observational, analytical, case-control study. We performed genetic analysis to evaluate various genetic polymorphisms and analyzed cytokine levels at admission.

Results: 429 patients with ischemic stroke and 195 control subjects without ischemic stroke. Patients with CEI subtype showed significantly higher levels of all the cytokines analyzed, namely, IL-10, TNF-alpha, and IL-6. Logistic regression analysis revealed that higher TNF-α (alpha), IL-6, and IL-1 β levels were significantly associated with the LAAS and CEI subtypes. TNF-α, IL-1, and IL-6 levels were significantly higher in patients with recurrent stroke at follow-up. Of the three polymorphisms in the gene encoding PTSG2, the haplotypes rs6275 and rs20417 showed a different distribution between patients and controls.

Discussion: The reported association between ischemic stroke and immunoinflammatory variables agrees with previously reported associations between some proinflammatory and prothrombotic polymorphisms and the risk of ischemic stroke.

Introduction: Phosphoinositide-3-kinase δ (PI3Kδ) is a critical regulator of cellular processes, predominantly within the immune system, and its dysregulation is implicated in inborn errors of immunity, including Activated PI3 Kinase Delta Syndrome (APDS), and various malignancies, including hematological and solid tumors. Given PI3Kδ's predominant expression in leukocytes, its aberrant activation not only promotes tumor growth but also enables immune evasion, underscoring its importance as an 'immune-check pathway,' especially in immune-rich tumor microenvironments.

Areas covered: This review examines the oncogenic role of PI3Kδ signaling, emphasizing its contribution to immune evasion, tumor proliferation, survival, and metastasis across different cancers. A bibliographic search was conducted in PubMed, Science Direct, MEDES and SciELO using specific criteria for articles published until 2023.

Expert opinion: PI3Kδ signaling can be conceptualized as an immune check pathway, reinforcing its potential as a therapeutic cancer target. Recent clinical trials using PI3Kδ inhibitors have shown efficacy across various cancers (hematological, breast, gynecologic, and digestive cancers), suggesting their applicability beyond APDS and primary malignancies. This approach could prevent cancer-linked immunodeficiency, providing dual therapeutic and prophylactic benefits. Future research should explore PI3Kδ inhibitors as a comprehensive strategy for managing cancer progression and associated immune dysfunction, ultimately improving patient outcomes.