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Functional Profile of Enzymatic Hydrolysates in Food Proteins 食品蛋白质中酶水解物的功能简介
Q3 Economics, Econometrics and Finance Pub Date : 2024-07-03 DOI: 10.21603/2074-9414-2024-2-2515
Svetlana Lebedeva, B. Bolkhonov, S. Zhamsaranova, B. Bazhenova, S. Leskova
Food allergy is a matter of global concern, proteins being a popular allergen worldwide. Enzymatic protein hydrolysates serve as hypoallergenic components in functional foods. This article describes enzymatic hydrolysates of egg and soy proteins as potential antigens, sensitizers, and antioxidants. The research featured soy protein isolate and egg albumin. The protein hydrolysates were obtained in a two-step process with pepsin and trypsin to measure hydrolysis, total antioxidant activity, and residual antigenicity. The sensitizing ability of native proteins and their hydrolysates was studied using a delayed-type hypersensitivity (DTH) skin test of paw swelling in mice. The egg albumin and soy protein were hydrolyzed by sequentially adding the proteases, i.e., pepsin and trypsin. The degree of protein hydrolysis was calculated against total nitrogen and its contents in non-hydrolyzed raw materials and hydrolysate. It was 82.6% for the egg hydrolysate and 88.3% for the soy hydrolysate, the total antioxidant activity being 114.3 and 91.4 mg/100 mL, respectively. The residual antigenicity of the hydrolysates determined by ELISA in a sandwich version was 1.55×10–4 and 3.30×10–4 RU, respectively. The native egg and soy proteins demonstrated good allergenic properties, and the DTH response index increased fivefold. The hydrolysates reduced the DTH response indices by an average of 3.5 for egg hydrolysate and 2.6 times for soy hydrolysate. The resulting enzymatic hydrolysates of egg albumin and soy protein demonstrated a high degree of hydrolysis and antioxidant activity. They had low residual antigenicity (10–4) and low sensitizing ability. The resulting enzymatic hydrolysates could be used as hypoallergenic components and antioxidants in new functional foods.
食物过敏是一个全球关注的问题,蛋白质是全球流行的过敏原。酶水解蛋白是功能食品中的低过敏成分。本文介绍了作为潜在抗原、致敏剂和抗氧化剂的鸡蛋和大豆蛋白酶水解物。研究以大豆分离蛋白和鸡蛋白蛋白为特色。蛋白质水解物是通过胃蛋白酶和胰蛋白酶两步法获得的,以测量水解度、总抗氧化活性和残留抗原性。使用小鼠爪肿延迟型超敏反应(DTH)皮肤试验研究了原生蛋白质及其水解物的致敏能力。通过依次加入蛋白酶,即胃蛋白酶和胰蛋白酶,对鸡蛋白蛋白和大豆蛋白进行水解。根据未水解原料和水解物中的总氮及其含量计算蛋白质的水解程度。鸡蛋水解物的水解度为 82.6%,大豆水解物的水解度为 88.3%,总抗氧化活性分别为 114.3 毫克/100 毫升和 91.4 毫克/100 毫升。用酶联免疫吸附法测定的水解物残留抗原性分别为 1.55×10-4 和 3.30×10-4 RU。原生鸡蛋和大豆蛋白具有良好的致敏特性,DTH 反应指数增加了五倍。水解物的 DTH 反应指数平均降低了 3.5 倍(鸡蛋水解物)和 2.6 倍(大豆水解物)。鸡蛋白蛋白和大豆蛋白的酶水解产物具有很高的水解度和抗氧化活性。它们的残留抗原性低(10-4),致敏能力低。由此产生的酶水解物可用作新型功能食品中的低过敏成分和抗氧化剂。
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引用次数: 0
Mycelial Biomass Enzymes of Cordyceps militaris and Lentinula edodes in Baking Technology 冬虫夏草和冬菇菌丝体生物酶在烘焙技术中的应用
Q3 Economics, Econometrics and Finance Pub Date : 2024-07-03 DOI: 10.21603/2074-9414-2024-2-2502
Denis Minakov, S. Koneva, Elena Egorova
Cordyceps militaris and Lentinula edodes are known for their high extracellular proteolytic, amylolytic, and laccase activity, which is important for bakery production. This article describes the effect of enzymes obtained from mycelial biomass of C. militaris and L. edodes on such properties of flour mixes as amylolytic and proteolytic enzymic activity during baking, technological costs, and bread quality. The research featured strains of C. militaris SRG4 and L. edodes 3790 fungi; mycelial biomass powder of these fungi on a sterile grain substrate (rice, wheat); experimental flour mixes of wheat bread and first-grade baking flour with mycelial biomass powder; bread made from the experimental flour mixes. The experimental part included standard methods used in the bakery industry. The mycelial biomass of C. militaris and L. edodes fungi proved to be a promising baking additive because it possessed hydrolytic enzymes and was rich in protein substances (32.2 ± 1.5 and 26.4 ± 2.0%, respectively), specific mushroom polysaccharides (36.7 ± 0.8 and 52.2 ± 1.2%, respectively), and carotenoids (1600 ± 40 mcg/g of C. militaris biomass). The active amylases and proteinases in the mycelial biomass powder improved the sugar-forming ability and reduced the falling number. In addition, they raised the amount of washed gluten with a slight decrease in elasticity, as well as provided an acceptable structure and viscosity of the dough at 1–4% of mycelial biomass powder. This dose resulted in an optimal bread formulation of standard quality. A bigger amount caused the crumb to darken and increased its humidity while reducing the specific volume. The bread samples with C. militaris were too acid. Mycelial biomass of C. militaris and L. edodes proved to be a good source of enzymes to be used with grain substrates in bakery production. Further research is needed to define the optimal dose and processing modes.
冬虫夏草(Cordyceps militaris)和冬虫夏草(Lentinula edodes)以其较高的胞外蛋白分解、淀粉分解和漆酶活性而闻名,这对烘焙生产非常重要。这篇文章介绍了从米曲霉和小扁豆菌丝体生物质中提取的酶对混合面粉特性的影响,如烘焙过程中的淀粉分解酶和蛋白分解酶活性、技术成本和面包质量。研究的主要内容包括:军事真菌 SRG4 菌株和埃多德真菌 3790 菌株;这些真菌在无菌谷物基质(大米、小麦)上的菌丝体生物质粉末;小麦面包和一级烘焙面粉与菌丝体生物质粉末的实验性混合面粉;用实验性混合面粉制作的面包。实验部分包括烘焙业使用的标准方法。事实证明,蘑菇菌丝体生物质是一种很有前景的烘焙添加剂,因为它具有水解酶,富含蛋白质物质(分别为 32.2 ± 1.5% 和 26.4 ± 2.0%)、特定蘑菇多糖(分别为 36.7 ± 0.8% 和 52.2 ± 1.2%)和类胡萝卜素(1600 ± 40 微克/克蘑菇菌丝体生物质)。菌丝体生物质粉中的活性淀粉酶和蛋白酶提高了糖的形成能力,减少了下降数量。此外,菌丝体生物质粉中的活性淀粉酶和蛋白酶还提高了洗面筋的数量,但弹性略有下降,而且在菌丝体生物质粉的用量为 1-4% 时,面团的结构和粘度均可接受。这一剂量可制成标准质量的最佳面包配方。添加量越大,面包屑颜色越深,湿度越大,同时比容也会降低。含有米氏杆菌的面包样品酸度过高。事实证明,酵母菌和酵母菌的菌丝体生物质是一种良好的酶源,可与谷物基质一起用于面包生产。需要进一步研究确定最佳剂量和加工模式。
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引用次数: 0
Native Microbiota of Rapeseed Cake as Potential Source of Industrial Producers 油菜籽饼中的本地微生物群是工业生产者的潜在来源
Q3 Economics, Econometrics and Finance Pub Date : 2024-07-03 DOI: 10.21603/2074-9414-2024-2-2504
O. Sverdlova, Elizaveta Podshivalova, Natalya Sharova, Daria Belova
Rapeseed oil production is very active in Russia. Its main by-product is cake: its chemical composition and availability make it an excellent source of nutrients in microbial cultivation to be used as biologically active compounds or as part of functional products. The research objective was to study the native microbiota of rapeseed cake under conditions of high humidity, as well as to describe the morphology of isolated microorganisms and determine their genera by NGS sequencing. The study featured rapeseed cake and microbial colonies isolated after 2, 5, 7, and 9 days of fermentation. The microbial isolates were obtained by the method of surface and deep cultivation on solid and liquid nutrient media, respectively. The metagenomic analysis of the microbial taxonomy involved sequencing on the Illumina platform. The experiment revealed 16 types of colonies with different morphology. Colonies with morphology 1, 3, 8, 12, and 13 were predominant for all fermentation periods, which means that they grew as isolates during the liquid-phase fermentation. The metagenomic analysis revealed at least 28 genera of bacteria in the rapeseed cake suspensions. The highest percentage belonged to Weisella (≤ 45.8% on day 2), Acinetobacter (≤ 40.6% on day 7), Lactobacillus (≤ 15.7% on day 5), Leuconostoc (≤ 15.1% on day 7), Enterococcus (≤ 14.6% on day 5), and Paenibacillus (≤ 16.3% on day 9). The obtained isolates could be of interest as industrial producers of useful metabolites, e.g., enzymes, pigments, organic acids, etc. Further research will identify the microbial species, their useful properties, and optimal cultivation conditions.
俄罗斯的菜籽油生产非常活跃。其主要副产品是菜籽饼:菜籽饼的化学成分和可用性使其成为微生物培养的极佳营养来源,可用作生物活性化合物或功能性产品的一部分。研究目的是研究高湿度条件下油菜籽饼的本地微生物群,以及描述分离微生物的形态,并通过 NGS 测序确定其属种。研究对象为油菜籽饼和发酵 2、5、7 和 9 天后分离出的微生物菌落。微生物分离物分别通过在固体和液体营养培养基上进行表面培养和深层培养的方法获得。微生物分类的元基因组分析包括在 Illumina 平台上进行测序。实验发现了 16 种不同形态的菌落。形态为 1、3、8、12 和 13 的菌落在所有发酵期都占优势,这意味着它们是在液相发酵过程中生长的分离菌。元基因组分析显示菜籽饼悬浮液中至少有 28 个细菌属。其中比例最高的是魏氏菌(第 2 天≤ 45.8%)、醋酐菌(第 7 天≤ 40.6%)、乳杆菌(第 5 天≤ 15.7%)、白念珠菌(第 7 天≤ 15.1%)、肠球菌(第 5 天≤ 14.6%)和芽孢杆菌(第 9 天≤ 16.3%)。获得的分离物可能是有用代谢物(如酶、色素、有机酸等)的工业生产者。进一步的研究将确定微生物种类、其有用特性和最佳培养条件。
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引用次数: 0
Combined Ultrasound and Microwave Food Processing: Efficiency Review 超声波和微波相结合的食品加工:效率回顾
Q3 Economics, Econometrics and Finance Pub Date : 2024-07-03 DOI: 10.21603/2074-9414-2024-2-2510
L. Burak, A. Zavaley
Modern methods of food processing combine ultrasonic and microwave treatment. This article reviews scientific publications on combined microwave and ultrasound technologies in food processing. The review involved standard methods of data analysis and covered 85 Scopus and Web of Science research papers on combined microwave and ultrasonic food treatment published in English in 2010–2023. The article describes the principles and specific features of ultrasonic and microwave food processing methods, their combinations, equipment design, and applications as part of various food processing stages, e.g., defrosting, drying, extraction, sterilization, etc. Microwaves increase the heating rate while ultrasound increases the efficiency of heat and mass transfer. Their combined use reduces processing time, improves product quality, facilitates moisture drainage, and provides uniform heating. The combined effect of microwaves and ultrasound increases the processing efficiency, thus preserving the nutritional value and quality of the final product. Ultrasonic technology serves as an aid to efficient and environmentally-friendly microwave heating, which has a wide range of applications in the food industry. The review can be used in further research in extraction, drying, defrosting, and sterilization. It can help food industry specialists to select optimal food processing methods.
现代食品加工方法结合了超声波和微波处理技术。本文回顾了有关食品加工中微波和超声波组合技术的科学出版物。综述采用了标准的数据分析方法,涵盖了 2010-2023 年间发表的 85 篇有关微波和超声波联合处理食品的 Scopus 和 Web of Science 英文研究论文。文章介绍了超声波和微波食品加工方法的原理和具体特点、其组合、设备设计以及在解冻、干燥、萃取、灭菌等不同食品加工阶段的应用。微波能提高加热速度,而超声波能提高热量和质量的传递效率。两者结合使用可缩短加工时间,提高产品质量,促进水分排出,并提供均匀的加热。微波和超声波的共同作用提高了加工效率,从而保持了最终产品的营养价值和质量。超声波技术是高效、环保的微波加热技术的辅助手段,在食品工业中有着广泛的应用。本综述可用于提取、干燥、解冻和灭菌方面的进一步研究。它可以帮助食品工业专家选择最佳的食品加工方法。
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引用次数: 0
Global Market of Chemical Crop Protection Agents: Forecasting Yield Losses and Economic Prospects for Domestic Pesticide Production 全球化学农药保护剂市场:预测国内农药生产的产量损失和经济前景
Q3 Economics, Econometrics and Finance Pub Date : 2024-07-03 DOI: 10.21603/2074-9414-2024-2-2508
Alexander Tareev, Alexey Bereznov, Valery Smirnov, A. Tareeva, Sofya Kislaya
To ensure its food security and economic growth, Russia needs to monitor the global pesticide market because constant monitoring predicts supply stability and provides annual high yields. The article describes the current issues associated with agricultural pesticide, analyzes the development directions on the global pesticide market, and predicts its impact on the Russian economy. To analyze the global market of chemical plant protection products, the authors applied an integrated approach based on analysis, synthesis, analogy, comparison, and forecasting. The research relied on the domestic laws and standards issued by the Ministry of Agriculture and the Federal Service for Veterinary and Phytosanitary Surveillance. Other sources included statistics obtained from foreign agencies (Phillips McDougal; Kynetec; NBS, China; Chemexcil, India) and Russian institutions (Rosstat, BusinesStat, Zerno.ru, etc.). The review included articles published in scientific and analytical databases, i.e., eLIBRARY.RU, AgroXXI, and AgroPages in 2018–2024. The analysis concentrated on India, China, and the Russian Federation. It covered absolute and relative harvest losses, as well as the market share of importing countries and key suppliers on Russian pesticide market. The demand for chemical pesticides continues to grow, despite restrictions or prohibitions of some hazardous pesticides. Some active ingredients were partially unavailable while finished formulations were subjected to import quotas. In 2024, Russia will be using as many chemical plant protection products as in 2023 since the domestic warehouses store large reserves. The weather conditions are likely to ensure optimal plant growth. Russian farmers will harvest about 142 million tons of grain, including 92–95 million tons of wheat. If repeated annually, this research may reduce the risk of harvest losses and allow the state to adopt prompt regulation measures to obtain high-quality products, thus increasing the domestic consumption and export potential.
为确保粮食安全和经济增长,俄罗斯需要对全球农药市场进行监控,因为持续监控可预测供应的稳定性并提供年度高产。文章介绍了当前与农业农药相关的问题,分析了全球农药市场的发展方向,并预测了其对俄罗斯经济的影响。为了分析全球化学植保产品市场,作者采用了基于分析、综合、类比、比较和预测的综合方法。研究依据的是农业部和联邦兽医和植物检疫监督局颁布的国内法律和标准。其他来源包括从外国机构(Phillips McDougal、Kynetec、中国国家统计局、印度 Chemexcil)和俄罗斯机构(Rosstat、BusinesStat、Zerno.ru 等)获得的统计数据。综述包括 2018-2024 年在科学和分析数据库(即 eLIBRARY.RU、AgroXXI 和 AgroPages)中发表的文章。分析主要集中在印度、中国和俄罗斯联邦。它涵盖了绝对和相对收成损失,以及俄罗斯农药市场进口国和主要供应商的市场份额。尽管某些危险农药受到限制或禁止,但对化学农药的需求仍在继续增长。部分活性成分无法获得,而成品制剂则受进口配额限制。2024 年,俄罗斯使用的化学植保产品将与 2023 年一样多,因为国内仓库储存了大量的化学植保产品。天气条件可能会确保植物的最佳生长。俄罗斯农民将收获约 1.42 亿吨谷物,其中包括 9200-9500 万吨小麦。如果每年重复进行这项研究,就可以降低收获损失的风险,使国家能够及时采取调控措施,获得优质产品,从而提高国内消费和出口潜力。
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引用次数: 0
Removing Excess Iron from Sewage and Natural Waters: Selecting Optimal Sorbent 去除污水和自然水体中的过量铁:选择最佳吸附剂
Q3 Economics, Econometrics and Finance Pub Date : 2024-07-03 DOI: 10.21603/2074-9414-2024-2-2516
Ludmila Ivanova, I. Timoshchuk, Alena Gorelkina, Ekaterina Mikhaylova, Nadezhda Golubeva, E. Neverov, Tamara Utrobina
Natural waters and wastewaters often contain heavy metals, e.g., iron. Iron ore mining contaminates groundwater with iron up to 30 maximal permissible concentrations (MPC) as this element gets washed out from rock and soil. Adsorption is the most effective and economically feasible method of additional purification of natural and wastewater from iron. Its efficiency depends on the type of adsorbent. The research objective was to select the most efficient sorption material to eliminate water from iron, as well as to establish the adsorption patterns for different sorbents, thus creating sustainable and effective purification. The study featured carbonaceous sorbent of the SKD-515 grade, mineral sorption materials with aluminosilicate of the AC grade, and silicate-based sorbent of the ODM-2F grade. The porous structure was studied by porometry methods while the surface image was obtained using scanning electron microscopy. Other indicators included equilibrium, kinetics, and dynamics of iron adsorption by various sorbents. The Freundlich and Langmuir equations made it possible to calculate the key adsorption parameters. The Gibbs energy values were obtained from the Langmuir equation and equaled 11.93–20.66 kJ/mol, which indicated the physical nature of the adsorption process. Under static conditions, the sorbents demonstrated a high adsorption capacity with respect to iron, depending on the structure, and could be arranged as AC > SKD-515 > ODM-2F. In SKD-515, iron adsorption occurred in micropores; in AC and ODM-2F, it took place in mesopores. The kinetics of iron extraction showed that the adsorption process was limited by external mass transfer. The research provided a new understanding of iron adsorption by materials of various structures. The conclusions were supported by scanning electron microscopy images. Initial concentration, flow velocity, and loading layer height were studied in dynamics, i.e., during continuous operation of the adsorption column. The system proved extremely effective and reached 99.0% Fe3+ extraction under the following conditions: flow rate = 1 L/min, loading column height = 0.15 m, column diameter = 0.05 m, initial concentration = 0.5 mg/L (5 MPC). The column performance was tested at an initial concentration of iron ions of 50 MPC, which simulated the wastewater treatment at industrial enterprises. This comprehensive study of iron adsorption from wastewater proved the efficiency of the mineral sorption materials with aluminosilicate of AC grade.
自然水体和废水通常含有重金属,如铁。铁矿石开采过程中,铁元素会从岩石和土壤中被冲刷出来,从而污染地下水,其最大允许浓度(MPC)可达 30。吸附是额外净化天然水和废水中铁含量的最有效、最经济可行的方法。其效率取决于吸附剂的类型。研究的目的是选择最有效的吸附材料来消除水中的铁,并确定不同吸附剂的吸附模式,从而实现可持续的有效净化。研究采用了 SKD-515 级碳质吸附剂、AC 级硅酸铝矿物吸附材料和 ODM-2F 级硅酸盐吸附剂。多孔结构是通过孔隙度测量方法进行研究的,而表面图像则是通过扫描电子显微镜获得的。其他指标包括各种吸附剂对铁的吸附平衡、动力学和动力学。利用 Freundlich 和 Langmuir 方程可以计算出关键的吸附参数。根据 Langmuir 方程得出的吉布斯能值为 11.93-20.66 kJ/mol,这表明了吸附过程的物理本质。在静态条件下,不同结构的吸附剂对铁的吸附能力不同,可按 AC > SKD-515 > ODM-2F 排序。在 SKD-515 中,铁的吸附发生在微孔中;在 AC 和 ODM-2F 中,铁的吸附发生在中孔中。铁萃取动力学表明,吸附过程受到外部传质的限制。这项研究使人们对不同结构材料的铁吸附有了新的认识。结论得到了扫描电子显微镜图像的支持。对初始浓度、流速和负载层高度进行了动态研究,即吸附塔连续运行期间的研究。事实证明,该系统非常有效,在以下条件下可达到 99.0% 的 Fe3+ 萃取率:流速 = 1 升/分钟,吸附柱高度 = 0.15 米,吸附柱直径 = 0.05 米,初始浓度 = 0.5 毫克/升(5 MPC)。在铁离子初始浓度为 50 MPC 的条件下测试了柱的性能,模拟了工业企业的废水处理情况。这项关于废水中铁吸附的综合研究证明了 AC 级硅酸铝矿物吸附材料的效率。
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引用次数: 0
Histological Studies of Sous Vide Beef Based on Best Practices in Meat Quality Formation 基于肉质形成最佳实践的生鲜牛肉组织学研究
Q3 Economics, Econometrics and Finance Pub Date : 2024-07-03 DOI: 10.21603/2074-9414-2024-2-2514
A. Koksharov, L. Mayurnikova, A. Plastun, N. Davydenko, T. Krapiva
Sous vide technology came to Russia in the early 2000s. Its quality standards require a multidimensional comprehensive study to develop theoretical and methodological foundations. The research featured the effect of various Sous vide modes on beef quality based on histological analysis and sensory assessment. The review of best practices in this sphere included publications registered in Scopus, Web of Science, and eLIBRARY.RU. Beef from sacral part underwent Sous vide processing at two modes, i.e., 57 and 64°C for 24 h and 80°C for 12 h. The samples obtained were subjected to histological analysis and sensory assessment. The histology showed that the cooking process at 100°C and the Sous vide modes at 57, 64, and 80°C increased the intercellular space and dissolved the connective tissue. Higher temperature modes led to more pronounced shrinking and deformation of muscle fibers. Under the Sous vide conditions, fibrous structures demonstrated the most severe destruction after 12 h at 80°C. As the destruction of muscle fibers increased, more nuclei entered intercellular space. Moreover, perimysium and sarcolemma demonstrated signs of granulation between muscle fiber bundles. The Sous vide samples received the best score for juiciness and tenderness. The research revealed a certain contradiction between the growing degree of fibrous destruction caused by thermal treatment and the improving consistency index for juiciness and tenderness. The authors consider it necessary to investigate the matter further and invite specialists to submit their ideas.
苏式烹调技术于 2000 年代初传入俄罗斯。其质量标准要求进行多维度的综合研究,以建立理论和方法基础。这项研究以组织学分析和感官评估为基础,探讨了各种 "苏氏蒸煮 "模式对牛肉质量的影响。对该领域最佳实践的审查包括在 Scopus、Web of Science 和 eLIBRARY.RU 上注册的出版物。在两种模式下对骶骨部位的牛肉进行了 "苏氏蒸煮 "处理,即 57 和 64°C 24 小时和 80°C 12 小时。组织学分析表明,100°C的烹饪过程和57、64和80°C的 "Sous vide "模式增加了细胞间隙,溶解了结缔组织。温度越高,肌肉纤维的收缩和变形越明显。在苏氏蒸煮条件下,纤维结构在 80°C 温度下 12 小时后的破坏最为严重。随着肌纤维破坏程度的增加,更多的细胞核进入细胞间隙。此外,肌周膜和肌浆表现出肌纤维束之间的肉芽肿迹象。苏氏蒸煮的样品在多汁度和嫩度方面得分最高。研究表明,热处理造成的纤维破坏程度增加与多汁性和嫩度的一致性指数提高之间存在一定的矛盾。作者认为有必要进一步研究这个问题,并邀请专家们提出他们的想法。
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引用次数: 0
Screening and Profiling the Antioxidant Properties of Psychrophilic Microalgae and Cyanobacteria from the Baltic Sea 筛选和分析波罗的海亲心理微藻和蓝藻的抗氧化特性
Q3 Economics, Econometrics and Finance Pub Date : 2024-07-03 DOI: 10.21603/2074-9414-2024-2-2501
V. Dolganyuk, S. Sukhikh, Egor Kashirskich, E. Ulrikh, O. Kremleva, Olga O. Babich
At present, the issue of obtaining a complex of biologically active substances with antioxidant activity from psychrophilic Psychrophilic microalgae and cyanobacteria are a prospective source of biologically active antioxidant substances. New antioxidant complexes could expand the scope of their industrial application. The research objective was to identify psychrophilic microalgae and cyanobacteria from the Baltic Sea in order to study their antioxidant properties. The research featured psychrophilic microalgae and cyanobacteria obtained from the Curonian Lagoon and the Baltic Bay in the Baltic Sea, Kaliningrad Region, Russia, in March – May 2022. The authors used the Pasteur method and the flow cytometry method to isolate pure cultures of psychrophilic microalgae and cyanobacteria. The method of spectrophotometry made it possible to study the antioxidant activity by analyzing radical scavenging, reducing ability, and chelating. The psychrophilic microalga Skeletonema pseudocostatum demonstrated the highest antioxidant activity in all three methods: 17.62 μmol/g trolox equivalents according to the ABTS method, 58.16 μmol/g trolox equivalents according to the DPPH method, and 3.91 μmol/g trolox equivalents according to the FRAP method. The psychrophilic microalga Thalassiosira pseudonana exhibited the following values of antioxidant activity: 12.08, 12.42, and 3.13 μmol/g trolox equivalents, respectively. The antioxidant activity of the psychrophilic microalgae Fragilariopsis kerguelensis was 13.53, 11.84, and 1.09 μmol/g trolox equivalents, respectively. The antioxidant activity of the psychrophilic cyanobacterium Aphanizomenon gracile was 15.73, 19.89, and 2.47 μmol/g trolox equivalents, respectively. The antioxidant activity of the psychrophilic cyanobacterium Anabaena cylindrica was 12.62, 13.16, and 2.16 μmol/g trolox equivalents, respectively. The samples of psychrophilic microalgae and cyanobacteria obtained from natural environment in the Russian Baltic Sea demonstrated good antioxidant properties, which makes them a potential raw material for new pharmaceutical substances.
目前,从亲心理微藻和蓝藻中获得具有抗氧化活性的生物活性物质复合物的问题是一个具有前景的生物活性抗氧化物质来源。新的抗氧化剂复合物可以扩大其工业应用范围。研究目的是鉴定波罗的海的嗜心理微藻和蓝藻,以研究它们的抗氧化特性。该研究以 2022 年 3 月至 5 月从俄罗斯加里宁格勒州波罗的海库伦泻湖和波罗的海湾获得的嗜灵性微藻和蓝藻为特色。作者采用巴斯德法和流式细胞仪法分离出了嗜心理微藻和蓝藻的纯培养物。分光光度法通过分析自由基清除能力、还原能力和螯合能力来研究抗氧化活性。在所有三种方法中,嗜心理微藻 Skeletonema pseudocostatum 的抗氧化活性最高:ABTS 法为 17.62 μmol/g trolox 当量,DPPH 法为 58.16 μmol/g trolox 当量,FRAP 法为 3.91 μmol/g trolox 当量。亲水微藻 Thalassiosira pseudonana 的抗氧化活性值分别为 12.08、12.42 和 3.13 μmol/g 三氧化二氮当量。嗜碱性微藻 Fragilariopsis kerguelensis 的抗氧化活性分别为 13.53、11.84 和 1.09 μmol/g 托罗克斯当量。Aphanizomenon gracile 的抗氧化活性分别为 15.73、19.89 和 2.47 μmol/g 托罗克斯当量。亲水蓝藻 Anabaena cylindrica 的抗氧化活性分别为 12.62、13.16 和 2.16 μmol/g 托罗克斯当量。从俄罗斯波罗的海自然环境中获得的嗜灵性微藻和蓝藻样本表现出良好的抗氧化性,这使它们成为新医药物质的潜在原料。
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引用次数: 0
Drying Grain Pretreated by Low-Voltage Spark-Discharge Plasma Channel 用低压火花放电等离子体通道预处理谷物的干燥方法
Q3 Economics, Econometrics and Finance Pub Date : 2024-03-28 DOI: 10.21603/2074-9414-2024-1-2493
Emad Hussein Ali Munassar, I. Shorstkii
The food industry and agriculture use such electrophysical technologies as ozonation, pulsed electric field, and low-temperature plasma. They increase the shelf-life of food products, as well as help to advance food processing. This article features pretreatment with a low-voltage spark-discharge plasma channel as a means to increase the efficiency of grain drying. The grain material involved three samples of soft wheat seeds. Sample 1 was subjected to direct contact with the electrodes while sample 2 underwent treatment on a dielectric substrate. The control remained untreated. The kinetics of grain-drying in the open air was studied using a thermal agent at 110°C after pre-treatment with a low-voltage spark-discharge plasma channel. This experiment also involved scanning electron microscopy tools to detect changes in surface structure. The electron microscopy showed that the dielectric substrate accelerated moisture removal, probably as a result of the emerging surface effects that developed a new continuum in the grain structure. This treatment made it possible to reduce the drying time by 15–25%, compared to the control sample. The drying rate curves demonstrated acceleration in the initial period, associated with additional electroosmotic forces and changes in the absorption properties. The samples treated with low-voltage spark-discharge plasma channel showed a 20% reduction in total energy consumption. Electrophysical technology based on a low-voltage spark-discharge plasma channel proved to be an effective pre-drying procedure. Further research is needed to scale the technology in a flow mode and to identify its effect on shelf-life.
食品工业和农业使用臭氧、脉冲电场和低温等离子体等电物理技术。这些技术可延长食品的保质期,并有助于推进食品加工。本文介绍了利用低压火花放电等离子通道进行预处理以提高谷物干燥效率的方法。谷物材料涉及三个软小麦种子样品。样品 1 与电极直接接触,而样品 2 则在电介质基板上进行处理。对照组未经处理。在使用低压火花放电等离子通道进行预处理后,在 110°C 温度下使用热敏剂对谷物在露天干燥的动力学进行了研究。该实验还使用了扫描电子显微镜工具来检测表面结构的变化。电子显微镜显示,电介质基底加速了水分的去除,这可能是由于新出现的表面效应在晶粒结构中形成了新的连续体。与对照样品相比,这种处理方法使干燥时间缩短了 15-25%。干燥速率曲线显示,在初始阶段干燥速率加快,这与额外的电渗力和吸收特性的变化有关。使用低压火花放电等离子通道处理的样品总能耗降低了 20%。事实证明,基于低压火花放电等离子通道的电物理技术是一种有效的预干燥程序。需要进一步研究如何在流动模式下推广该技术,并确定其对货架期的影响。
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引用次数: 0
Quails of Stavropol Region: Autumn Food Habits 斯塔夫罗波尔地区的鹌鹑秋季食性
Q3 Economics, Econometrics and Finance Pub Date : 2024-03-28 DOI: 10.21603/2074-9414-2024-1-2489
Anatoly Kaledin, L. Malovichko, Alexander Rezanov, L.S. Drozdova, A. Serikbayeva
Quails (Coturnix coturnix (Linnaeus, 1758)) are the only migratory Galliformes species. Quails are nestling ground-dwelling birds, feeding on seeds and crawling insects. Wild quails inhabit many parts of Russia. Quail farming is quite popular because quail meat is low in calories and possesses a number of beneficial properties, e.g., it is rich in proteins, contains little fat and virtually no carbohydrates, etc. As a rule, quails winter in Transcaucasia and Southern Europe; however, they sometimes prefer to stay in southern regions for the winter. This article introduces the autumn food habits of quails in the Central Ciscaucasia. The research relied on the analysis of stomach contents of 156 birds killed by hunters or hit by vehicles in several districts of the Stavropol Region. The material was collected in the autumn in 2008–2021. In the Central Ciscaucasia, quails are mesophiles and inhabit abandoned wheat and pea fields. The population fluctuates from year to year, depending on spring weather conditions. Their optimal nestling grounds include irrigated fields and areas near canals and ponds. In the steppe regions, they prefer agricultural areas, e.g., orchards, vineyards, holiday villages, sheep sheds, green belts along fields, roads, and railways, etc. Being phytophages, they rely on plant and, to a lesser extent, animal forage. In the autumn, they feeding on green plants, wheat grain, seeds of cultivated and wild plants, and insect larvae.
鹌鹑(Coturnix coturnix (Linnaeus, 1758))是唯一的迁徙性瘿蚊目物种。鹌鹑是筑巢的地栖鸟类,以种子和爬行昆虫为食。野生鹌鹑栖息在俄罗斯的许多地方。鹌鹑养殖很受欢迎,因为鹌鹑肉热量低,具有许多有益的特性,如富含蛋白质、脂肪含量低、几乎不含碳水化合物等。鹌鹑通常在外高加索和南欧过冬,但有时也喜欢在南方地区过冬。本文介绍了中西高加索地区鹌鹑秋季的饮食习惯。研究依据的是对斯塔夫罗波尔州多个地区被猎人打死或被车辆撞死的 156 只鸟类的胃内容物分析。这些材料是在2008-2021年秋季收集的。在中西高加索地区,鹌鹑属于中栖动物,栖息在废弃的麦田和豌豆田中。鹌鹑的数量每年都有波动,这取决于春季的天气状况。它们的最佳筑巢地包括灌溉田以及运河和池塘附近地区。在草原地区,它们更喜欢农业区,如果园、葡萄园、度假村、羊舍、田间绿化带、公路和铁路沿线等。作为植食性动物,它们依赖植物饲料,其次是动物饲料。秋季,它们以绿色植物、麦粒、栽培植物和野生植物的种子以及昆虫幼虫为食。
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引用次数: 0
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Food Processing: Techniques and Technology
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