{"title":"Research Article MQS-PCR: a simple and quick multiplex PCR molecular method for human chromosomal sex determination in DNA samples","authors":"J. Rocha, H. B. Pena, S. Pena","doi":"10.4238/gmr19067","DOIUrl":"https://doi.org/10.4238/gmr19067","url":null,"abstract":"","PeriodicalId":12518,"journal":{"name":"Genetics and Molecular Research","volume":"1 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70936692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Aksoy, A. Varoğlu, E. Onalan, A. Tektemur, G. Artas
It is known that mesothelin (MSLN) is overexpressed in some cancers, and that it plays a role in cell growth through Wnt family member 1 protein. Malignant transformation usually occurs with disruption of autoregulation of autophagy-related genes. We examined the effect of MSLN on survival and clinicopathological features in mesothelioma cases, as well as variations in genes associated with autophagy, invasion, apoptosis-related genes after siRNA-mediated MSLN silencing transfection in a mesothelioma cell line (SPC212). MSLN expression was analyzed, immunohistochemically, in formalinfixed paraffin-embedded 60 mesothelioma cases. MSLN expression was categorized by median MSLN histoscores as low (L), high (H). The correlation between the levels of MSLN expression, clinicopathological features, and survival was determined in mesothelioma cases. The siRNA-mediated MSLN incubated SPC212 cells were transfected and compared to negative control siRNAs. mRNA levels were determined for autophagy, invasion, and apoptosis related-genes with RT-PCR, and western blotting in SPC212 cells after MSLN silencing. All of the cases were immunoreactive for MSLN expression. H-MSLN was associated with a favorable prognosis according to Kaplan-Meier survival analysis, but Cox regression analysis revealed that only stage was a significant independent factor for estimating survival. MSLN overexpression was
{"title":"Research Article siRNA-mediated mesothelin silencing for treatment of mesothelioma","authors":"A. Aksoy, A. Varoğlu, E. Onalan, A. Tektemur, G. Artas","doi":"10.4238/gmr18955","DOIUrl":"https://doi.org/10.4238/gmr18955","url":null,"abstract":"It is known that mesothelin (MSLN) is overexpressed in some cancers, and that it plays a role in cell growth through Wnt family member 1 protein. Malignant transformation usually occurs with disruption of autoregulation of autophagy-related genes. We examined the effect of MSLN on survival and clinicopathological features in mesothelioma cases, as well as variations in genes associated with autophagy, invasion, apoptosis-related genes after siRNA-mediated MSLN silencing transfection in a mesothelioma cell line (SPC212). MSLN expression was analyzed, immunohistochemically, in formalinfixed paraffin-embedded 60 mesothelioma cases. MSLN expression was categorized by median MSLN histoscores as low (L), high (H). The correlation between the levels of MSLN expression, clinicopathological features, and survival was determined in mesothelioma cases. The siRNA-mediated MSLN incubated SPC212 cells were transfected and compared to negative control siRNAs. mRNA levels were determined for autophagy, invasion, and apoptosis related-genes with RT-PCR, and western blotting in SPC212 cells after MSLN silencing. All of the cases were immunoreactive for MSLN expression. H-MSLN was associated with a favorable prognosis according to Kaplan-Meier survival analysis, but Cox regression analysis revealed that only stage was a significant independent factor for estimating survival. MSLN overexpression was","PeriodicalId":12518,"journal":{"name":"Genetics and Molecular Research","volume":"1 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70934601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Research Article Phylogenetic analyses and species delimitation in the section Corydalis (Papaveraceae) based on chloroplast markers","authors":"A. Oh","doi":"10.4238/gmr18996","DOIUrl":"https://doi.org/10.4238/gmr18996","url":null,"abstract":"","PeriodicalId":12518,"journal":{"name":"Genetics and Molecular Research","volume":"1 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70935002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. S. Steinberg, M. Carvalho, I. Rosse, M. A. Machado, M. Peixoto
{"title":"Research Article Polymorphisms in genes coding milk proteins and protein hormones involved in milk production traits in Brazilian Guzerá cattle","authors":"R. S. Steinberg, M. Carvalho, I. Rosse, M. A. Machado, M. Peixoto","doi":"10.4238/gmr19046","DOIUrl":"https://doi.org/10.4238/gmr19046","url":null,"abstract":"","PeriodicalId":12518,"journal":{"name":"Genetics and Molecular Research","volume":"1 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70935864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Research Article Adaptability and stability parameters for grain production in pigeonpea lines in the Brazilian semiarid region","authors":"A. Costa, Cátia Santos","doi":"10.4238/gmr19066","DOIUrl":"https://doi.org/10.4238/gmr19066","url":null,"abstract":"","PeriodicalId":12518,"journal":{"name":"Genetics and Molecular Research","volume":"1 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70936618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. Borella, B. P. Brasileiro, A. D. de Azeredo, L. Ruaro, R. A. de Oliveira, J. C. B. Filho
{"title":"Research Article Resistance to orange rust associated with the G1 molecular marker in parents of Brazilian RB sugarcane varieties","authors":"J. Borella, B. P. Brasileiro, A. D. de Azeredo, L. Ruaro, R. A. de Oliveira, J. C. B. Filho","doi":"10.4238/gmr18980","DOIUrl":"https://doi.org/10.4238/gmr18980","url":null,"abstract":"","PeriodicalId":12518,"journal":{"name":"Genetics and Molecular Research","volume":"1 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70934768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G. Bevilacqua, A. P. Neto, B. Val, Carolina Azevedo, J. S. Sobrinho, P. Libório, W. S. Leite, S. H. Unêda-Trevisoli
. Wheat is considered a basic cereal for civilization with great economic importance for world agriculture. Currently, wheat is planted in the south, southeast and midwest regions of Brazil. Brazilian consumption of wheat will grow in the coming years due to population increase, which will require developing new cultivars for non-tradtional regions. We investigated commercial wheat genotypes grown at various sowing dates in a region with high temperature conditions. The experiment was conducted during the two summer and two autumn seasons. Sowings were March 10, March 20, April 1 and April 10. The agronomic traits (grain yield, plant height, spike size, total spikelets per spike and fertile spikelets per spike) for four commercial genotypes were evaluated. The experimental design was randomized blocks in a factorial scheme, corresponding to four genotypes, four sowing seasons and two years of cultivation. The second sowing year gave the best performance for the genotypes. Genotypes BR 18 and BRS 404 gave superior agronomic performance, standing out in the third and fourth sowing dates, under these culture conditions.
{"title":"Research Article Agronomic performance of wheat cultivars in different sowing dates under high temperature conditions in Brazil","authors":"G. Bevilacqua, A. P. Neto, B. Val, Carolina Azevedo, J. S. Sobrinho, P. Libório, W. S. Leite, S. H. Unêda-Trevisoli","doi":"10.4238/gmr18993","DOIUrl":"https://doi.org/10.4238/gmr18993","url":null,"abstract":". Wheat is considered a basic cereal for civilization with great economic importance for world agriculture. Currently, wheat is planted in the south, southeast and midwest regions of Brazil. Brazilian consumption of wheat will grow in the coming years due to population increase, which will require developing new cultivars for non-tradtional regions. We investigated commercial wheat genotypes grown at various sowing dates in a region with high temperature conditions. The experiment was conducted during the two summer and two autumn seasons. Sowings were March 10, March 20, April 1 and April 10. The agronomic traits (grain yield, plant height, spike size, total spikelets per spike and fertile spikelets per spike) for four commercial genotypes were evaluated. The experimental design was randomized blocks in a factorial scheme, corresponding to four genotypes, four sowing seasons and two years of cultivation. The second sowing year gave the best performance for the genotypes. Genotypes BR 18 and BRS 404 gave superior agronomic performance, standing out in the third and fourth sowing dates, under these culture conditions.","PeriodicalId":12518,"journal":{"name":"Genetics and Molecular Research","volume":"1 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70934945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. P. Ventorim, F. Rodrigues, L. A. Vianna, R. S. Dettogni, E. V. Santos, I. Louro, M. Oliveira-Silva
{"title":"Research Article Circulation and molecular characterization of Chikungunya virus (Togaviridae) reveals a new mutation (E2-N207D) in Espirito Santo, Brazil","authors":"D. P. Ventorim, F. Rodrigues, L. A. Vianna, R. S. Dettogni, E. V. Santos, I. Louro, M. Oliveira-Silva","doi":"10.4238/gmr18984","DOIUrl":"https://doi.org/10.4238/gmr18984","url":null,"abstract":"","PeriodicalId":12518,"journal":{"name":"Genetics and Molecular Research","volume":"41 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70935176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Research Article Isolation and characterization of DREB gene in ancestral diploid wheat species from a wheat domestication center","authors":"S. Rustamova, G. Abdullayeva, I. Huseynova","doi":"10.4238/gmr19035","DOIUrl":"https://doi.org/10.4238/gmr19035","url":null,"abstract":"","PeriodicalId":12518,"journal":{"name":"Genetics and Molecular Research","volume":"1 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70935826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E.S. Silva, A. Atroch, R. S. de Jesus, Monalisa Bitencourt, C. A. Ticona-Benavente
. The yam bean ( Pachyrhizus spp.) is an underutilized leguminous tuber that is well adapted to the Amazonian climate. This bean has 5 to 20% protein in its tuberous roots (dry weight), though it is little known even in Brazil. No improved varieties have been recommended for the Amazon region. We examine the Genotype x Year (GxY) interaction in 20 selected yam bean stocks and selected genotypes based on their performance. These materials were obtained from among a selection of 64 genotypes with natural outcrossing of P. erosus x P. tuberosus . The experiments were carried out in the Central Amazon during a low water period, from April to September in 2017 and 2018, in a completely randomized block design with 20 genotypes, three replicates, and eight plants per plot, significant GxY interaction, for pod and root yield. However, the carbohydrate, protein, lipid, fiber, and ash content in roots showed significant GxY interaction. These results indicate that selection of genotypes with high pod and root yield can be carried out in a single year in the dry period for cultivation during that season. However, selection to increase its nutritional value must be carried out over several years. The selected genotypes for presenting high root yield (> 3 t ha -1 ) were P7, P11, P15, P19, P37 and P57. The genotypes selected for high protein content in the roots (> 10% dry weight) were P13, P14, P37 and P62.
{"title":"Research Article Genotype x year interaction for agronomical traits and proximate composition in yam beans (Pachyrhizus spp., Fabaceae) in the Brazilian Amazon","authors":"E.S. Silva, A. Atroch, R. S. de Jesus, Monalisa Bitencourt, C. A. Ticona-Benavente","doi":"10.4238/gmr18981","DOIUrl":"https://doi.org/10.4238/gmr18981","url":null,"abstract":". The yam bean ( Pachyrhizus spp.) is an underutilized leguminous tuber that is well adapted to the Amazonian climate. This bean has 5 to 20% protein in its tuberous roots (dry weight), though it is little known even in Brazil. No improved varieties have been recommended for the Amazon region. We examine the Genotype x Year (GxY) interaction in 20 selected yam bean stocks and selected genotypes based on their performance. These materials were obtained from among a selection of 64 genotypes with natural outcrossing of P. erosus x P. tuberosus . The experiments were carried out in the Central Amazon during a low water period, from April to September in 2017 and 2018, in a completely randomized block design with 20 genotypes, three replicates, and eight plants per plot, significant GxY interaction, for pod and root yield. However, the carbohydrate, protein, lipid, fiber, and ash content in roots showed significant GxY interaction. These results indicate that selection of genotypes with high pod and root yield can be carried out in a single year in the dry period for cultivation during that season. However, selection to increase its nutritional value must be carried out over several years. The selected genotypes for presenting high root yield (> 3 t ha -1 ) were P7, P11, P15, P19, P37 and P57. The genotypes selected for high protein content in the roots (> 10% dry weight) were P13, P14, P37 and P62.","PeriodicalId":12518,"journal":{"name":"Genetics and Molecular Research","volume":"1 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70934450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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