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[Research on aseptic condition of root canals after endodontic treatment]. 【根管治疗后根管无菌状态的研究】。
N Taniguchi, M Fujisawa, K Shinohara, T Uetani, H Tadano, H Horiguchi, I Sekine

Fifty infected root canals were examined whether they had been in aseptic condition or not after endodontic treatment. PLADIA was used for the examination of bacteriologic culture. Twenty eight cases were positive, 22 cases were negative, and the percentage of positive was 56%. Of the anterior teeth, 13 cases were positive in 23 cases (57%), In the posterior teeth, 15 cases were positive in 27 cases (56%), 5 cases were positive in 17 cases (29%) with clinical symptoms before treatment, 23 cases were positive in 33 cases (70%) without clinical symptoms before treatment. These results showed that the treated root canals which had been well adapted to root canal filling by clinical symptoms had a high percentage of positive of bacteriological culture.

对50例感染的根管进行了根管治疗,检查其是否处于无菌状态。采用PLADIA进行细菌培养检查。阳性28例,阴性22例,阳性率为56%。前牙23例中阳性13例(57%),后牙27例中阳性15例(56%),治疗前有临床症状的17例中阳性5例(29%),治疗前无临床症状的33例中阳性23例(70%)。结果表明,经治疗的根管经临床症状适应后,其细菌培养阳性率较高。
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引用次数: 0
[Measuring of thresholds of the periodontal diseased teeth by electric pulp tester]. 【用电动牙髓测试仪测量牙周病变牙的阈值】。
H Hori, M Fujisawa, T Uetani, K Sinohara, H Tadano, H Horiguchi, I Sekine

Studying of influence of periodontal disease on vitality of pulp, the response thresholds of 421 teeth (250 normal teeth, 171 periodontal diseased teeth) were examined electronically by means of an automatic pulptester. (ANALYTIC TECHNOLOGY CO.) The pulp tester values of three experiments were averaged. The average value of normal teeth was 36.3 and that of periodontal diseased teeth 31.4. The response thresholds of periodontal diseased teeth were lower than those of normal teeth. This indicated that pulp was inflamed by periodontal disease and pulp vitality became weak.

为研究牙周病对牙髓活力的影响,采用自动牙髓测试仪对421颗牙(250颗正常牙和171颗牙周病变牙)的牙髓活力进行了电子检测。(ANALYTIC TECHNOLOGY CO.)三个实验的测浆值取平均值。正常牙的平均值为36.3,牙周病变牙的平均值为31.4。牙周病牙的反应阈值低于正常牙。这说明牙周病使牙髓发炎,牙髓活力变弱。
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引用次数: 0
[Color change by thickness and background color on visible light-cured composite resins]. 【可见光固化复合树脂厚度和底色的颜色变化】。
M Hotta, K Yamamoto, H Hirukawa, K Hashimoto, K Kondo, M Kondo, K Kimura

The effects of thickness and background color on the color of five commercial light-cured composite resins were studied by a dental color meter using the CIE 1976 L*a*b* color system. As thickness increased from 1.0 to 4.0 mm, values of L*a*b* decreased for white and dentin color background but showed no obvious difference in color for a black background. the color of composite resins were not in fluenced by background color in a thickness of 4.0 mm. The values of color difference for 1.0 and 2.0 mm thick samples have a larger value than the same 4.0 mm samples. The color difference was recognized even for the same shade name in five representative kinds of composite resins.

采用CIE 1976 L*a*b*显色系统,用牙用显色仪研究了厚度和底色对5种市售光固化复合树脂显色的影响。当厚度从1.0 mm增加到4.0 mm时,白色和牙本质颜色背景下的L*a*b*值降低,而黑色背景下的L*a*b*值无明显差异。在4.0 mm厚度范围内,复合树脂的颜色不受底色的影响。1.0 mm和2.0 mm厚样品的色差值比相同的4.0 mm样品的色差值大。在五种具有代表性的复合树脂中,即使是相同的色度名称也存在色差。
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引用次数: 0
[Biochemical studies on intramembranous localization of alkaline phosphatase in Bacillus megaterium KM]. [巨型芽孢杆菌(Bacillus megaterium KM)碱性磷酸酶膜内定位的生化研究]。
K Takahashi

Intramembranous localization of alkaline phosphatase (orthophosphoric monoester phosphohydrolase, alkaline optimum, EC 3, 1, 3, 1; AlPase) was observed biochemically in Bacillus megaterium KM grown in 1% polypeptone medium containing 0.5% NaCl at 37 degrees C under aerobic conditions and harvested at the latter logarithmic phase. AlPases from B. megaterium have been separated into soluble and membrane-bound forms by the centrifugation after cell disruption by sonication. The membrane-bound enzyme was further fractionated to two forms by phase separation using a non-ionic detergent, Triton X-114; one was successfully solubilized into the aqueous phase and the other remained in the Triton phase. Both AlPases of sonication- and Triton-solubilized forms were partially purified by gel filtration and anion-exchange column chromatographies. Their molecular weights were different (52,000 for soluble and 66,000 for Triton-solubilized forms) and the Vmax of the sonication-solubilized enzyme (227 nmol/min/mg protein) was 11-fold higher than that of the Triton-solubilized one although similar Km values (1.7 and 2.3 mM) were observed. Optimum pH of these enzymes tended to shift to a neutral range during the purification steps. These results suggest the multiplicity of AlPase anchoring to the membranes; 1) sonication-solubilized form which may be buried within the membrane lipids by its hydrophobic peptide and solubilized by the cell disruption, 2) detergent-solubilized form which may be bound loosely to the membrane by its hydrophobic domain and solubilized due to the amphiphilicity of enzyme protein, and 3) insolubilized form which may be bound fast to the membrane by its strong hydrophobicity and also have the function of enzymatic ability.

碱性磷酸酶(正磷酸单酯磷酸水解酶,alkaline optimum, EC 3,1,3,1)的膜内定位;在37℃有氧条件下,在含0.5% NaCl的1%多蛋白胨培养基中生长的巨型芽孢杆菌KM中,对AlPase进行了生化观察,并在对数后期收获。在超声破坏细胞后,通过离心分离得到了巨芽孢杆菌的可溶性和膜结合形式的AlPases。使用非离子洗涤剂Triton X-114将膜结合酶进一步分离成两种形式;一个被成功地溶解到水相中,另一个留在Triton相中。通过凝胶过滤和阴离子交换柱层析纯化了超声和triton溶解形式的AlPases。它们的分子量不同(可溶性为52,000,triton溶解形式为66,000),尽管Km值相似(1.7和2.3 mM),但超声溶解酶的Vmax (227 nmol/min/mg蛋白)比triton溶解酶高11倍。在纯化过程中,这些酶的最适pH值趋于中性。这些结果表明AlPase锚定在膜上的多样性;1)超声增溶型,可通过其疏水肽埋藏在膜脂中,并通过细胞破坏而溶解;2)洗涤剂增溶型,可通过其疏水结构域与膜松散结合,并由于酶蛋白的两亲性而溶解;3)不溶型,可通过其强疏水性与膜紧密结合,并具有酶促功能。
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引用次数: 0
[Acousticodynamic investigation of palatal rugae given by the pressure impression method]. [压印法对腭纹的声学动力学研究]。
K Ryumon, O Yamamura, Y Hayase, A Ogiso, T Tanigawa, E Sasaki, A Nakabayashi, H Matsui, T Noda, Y Marui

This time, we used palatal plates with the individual palatal rugae using the plastic plate for copying the individual palatal rugae easily, and studied the influence of the pronunciation concerning the changes of the Formant frequencies with Sound Spectrograph.

这次,我们使用带有单个腭纹的腭板,利用塑料板方便地复制单个腭纹,并利用声谱仪研究发音对共振峰频率变化的影响。
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引用次数: 0
[Reverse occlusion diagnosed following angle SN to FH correction]. 【经SN角到FH角矫正后诊断为反向闭塞】。
T Kawano, M Sakurai, M Matsumoto, M Sugiki, K Iba

A 12-year-old boy was first seen with the chief complaint of reverse occlusion. His records allowed a class III malocclusion, crown fracture of 1 angle and -1.1 degrees angle SN to FH. Therefore, we corrected angle SN to FH from -1.1 degrees to 7 degrees and treated him with intermaxillary anchorage appliance and by the edgewise method.

一名12岁男孩首次就诊,主诉为反向咬合。他的记录允许III级错牙合,冠骨折1角和-1.1角SN到FH。因此,我们将SN到FH的角度从-1.1度矫正到7度,并使用上颌间支抗器和边缘法治疗。
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引用次数: 0
[Acoustic investigation of various palatal rugae]. [各种腭纹的声学研究]。
S Mimura, O Yamamura, Y Hayase, T Tanigawa, E Sasaki, A Nakabayashi, H Matsui, M Takeuchi, K Ryumon, Y Maruie

We use some palatal plates with some Kinds of palatal rugae; ready made, patterned, individually copied and omitted, and studied the influence of the pronunciation acoustically concerning the Formant Frequency.

我们用一些腭板和一些腭纹;现成的,图案,单独复制和省略,并研究发音在声学上对共振频率的影响。
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引用次数: 0
Abrasion resistance of microwave-curing denture base resin. 微波固化义齿基托树脂的耐磨性。
K Okumura, M Yamauchi, T Koda, M Iwahori, M Sakai, J Kawano

This study was designed to test the abrasion resistance of microwave-curing denture base resin. We also studies the effects of surface smoothing treatment on abrasion resistance. Test resin was allowed to polymerize, and then buff polished or treated by surface smoothing. Knoop hardness was measured both prior to and following surface treatment. Then, the test material was subjected to brushing using a newly developed toothbrushing machine. Volume and surface roughness of the material were measured before and after brushing, and the surface structure of the material was examined with SEM. Microwave-curing denture base resin is equal in Knoop hardness and abrasion resistance to its conventional heat-curing counterpart, although it is smoother at the surface. The resin improved both in hardness and abrasion resistance when it underwent surface smoothing treatment regardless of whether it was heat-cured or microwave-cured. As was evident from SEM observations that both types of resins suffered superficial chipping following severe brushing, which is in accordance with our previous clinical findings.

本研究旨在测试微波固化义齿基托树脂的耐磨性。我们还研究了表面平滑处理对耐磨性的影响。测试树脂被允许聚合,然后抛光或表面平滑处理。测量表面处理前后的努氏硬度。然后,使用新开发的牙刷对测试材料进行刷牙。测量了刷刷前后材料的体积和表面粗糙度,并用扫描电镜观察了材料的表面结构。微波固化义齿基托树脂在努氏硬度和耐磨性方面与传统的热固化树脂相同,尽管它的表面更光滑。无论热固化还是微波固化,树脂经过表面光滑处理后,其硬度和耐磨性都得到了提高。从扫描电镜观察可以明显看出,两种类型的树脂在严重刷牙后都会出现表面脱落,这与我们之前的临床发现一致。
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引用次数: 0
[Studies on physico-chemical properties of self-setting apatite cement]. 自凝磷灰石水泥理化性能研究
Y Takezawa

A new dental cement was developed by use of tetracalcium phosphate monoxide (Te-CP) and dicalcium phosphate dihydrate (DCPD). When an equimolar mixture of the two calcium phosphates was mixed with diluted phosphoric acid, it hardened into a phase of hydroxyapatite (HAp) resembling the main inorganic phase of hard tissues. The present study was undertaken to investigate physico-chemical properties of this newly developed apatite cement and to evaluate its potential as dental cement to clinical application. The setting time was reduced in the presence of synthetic HAp, indicating added HAp to be an accelerator in the setting reaction. The pH of paste rapidly increased to a neutral range from initial solution acidity of about 2.0 and the subsequent setting reaction proceeded in a neutral and weak alkaline pH range. The hardened solid 4 hours after saturation was identified as single phase of apatite by X-ray diffraction. The HAp crystallinity of the set cement varied with the crystallinity of HAp added as setting accelerator. The wet compressive strength of the cement stored for one day in synthetic saliva at 37 degrees C increased to 400 kg/cm2 as added HAp crystallinity decreased. Moreover no disintegration took place when the cement was stored at 37 degrees C in synthetic saliva, which was undersaturated with respect to DCPD but well supersaturated for HAp. In clinical application to animals, no significant cleavage was observed between the hard tissues and the cement by SEM even three months after the cement paste was filled in the tooth cavity of monkeys. In the specimen prepared one week after implantation in the medullary canal of rats, no inflammatory cell appeared. The specimen prepared one month after implantation showed that the set cement was tightly contacted with newly formed bone. These findings strongly suggest that the newly developed self-setting apatite cement is useful as a pulp capping agents, root canal fillings and bone substitute.

以一氧化四磷酸钙(Te-CP)和二水合磷酸二钙(DCPD)为原料,研制了一种新型牙用骨水泥。当两种磷酸钙的等摩尔混合物与稀释的磷酸混合时,它硬化成羟基磷灰石(HAp)相,类似于硬组织的主要无机相。本研究旨在探讨这种新开发的磷灰石骨水泥的理化性质,并评价其作为牙用骨水泥的临床应用潜力。合成HAp存在时,凝结时间缩短,说明添加HAp在凝结反应中起促进作用。膏体的pH值从初始溶液酸度2.0左右迅速上升到中性范围,随后的凝固反应在中性和弱碱性pH范围内进行。饱和4h后的硬化固体经x射线衍射鉴定为磷灰石单相。水泥凝块的HAp结晶度随促进剂HAp结晶度的增加而变化。水泥在37℃合成唾液中保存1天的湿抗压强度随着添加HAp结晶度的降低而增加到400 kg/cm2。此外,当水泥在37℃的合成唾液中储存时,没有发生崩解,相对于DCPD是不饱和的,而相对于HAp是过饱和的。在动物临床应用中,即使将水泥浆充填于猴牙腔3个月后,扫描电镜也未观察到硬组织与骨水泥之间有明显的断裂现象。大鼠髓管内植入1周后的标本未见炎症细胞出现。植入1个月后的标本显示,固定骨水泥与新生骨接触紧密。这些发现有力地表明,新开发的自固化磷灰石胶粘剂是有用的髓盖剂,根管填充物和骨替代品。
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引用次数: 0
[Case report of maxillary protrusion]. 上颌前突1例。
S Yamamoto, J Wakayama, T Usuki, K Iba, K Mizutani

The patient, a 10-years and 7-month-old boy, demonstrated maxillary protrusion (a pseudo class I malocclusion) complicated by horizontal open bite, space defect of 5 5, and represented that point A was +3.0 mm, pogonion was -8.0 mm to McNamara line respectively. His 4 first premolars were extracted and he was treated with an edgewise appliance together with a high pull head gear, palatal bar, class III elastics, class II elastics and up-down elastics for 3 years. A functional occlusion and good profile was obtained with good growth, chin control and tooth movement.

患者男,10岁7个月,上颌前突(伪ⅰ类错颌)合并水平开咬,间隙缺损5.5,表示a点距麦克纳马拉线+3.0 mm,毒牙距麦克纳马拉线-8.0 mm。他的4颗第一前磨牙被拔了出来,他使用了一个边缘矫治器连同一个高拉头齿轮,腭棒,III类弹性,II类弹性和上下弹性进行了3年的治疗。在良好的生长、下巴控制和牙齿运动的情况下,获得了良好的功能性咬合和良好的轮廓。
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引用次数: 0
期刊
Gifu Shika Gakkai zasshi = The Journal of Gifu Dental Society
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