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Effective role of pommegranate peel extract on quality of tilapia fillets during storage 石榴皮提取物对罗非鱼鱼片贮藏品质的影响
Pub Date : 2022-12-23 DOI: 10.21608/mjvm.2022.181606.1015
G. Elsayed
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引用次数: 0
Prevalence of Bacillus in Heat treated milk and Infant food 芽孢杆菌在热处理牛奶和婴儿食品中的流行
Pub Date : 2022-12-23 DOI: 10.21608/mjvm.2022.163423.1011
B. Morsy, A. El-kholy, A. Meshref
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引用次数: 0
Unique cellular characteristics of the splenic marginal zone of the Barki sheep make it a powerful immunological defense 巴尔基羊脾脏边缘区独特的细胞特性使其具有强大的免疫防御作用
Pub Date : 2022-12-22 DOI: 10.21608/mjvm.2022.180156.1012
Omnya Elhussieny, Mohamed O. Abdelaziz, E. Khalifa
The marginal zone is a unique structure at the marginal area of the splenic white pulp, acting as a transient region where newly arriving cells, antigens, and blood are filtered. The splenic marginal zone of the Barki sheep surrounds the white pulp and is present between the white and red pulp. It is made up of marginal zone sinuses and diffuse lymphoid tissue aggregation peppered with smooth muscle cells and erythrocytes and supported by reticular cells. Rod-shaped endothelial cells seating on an interrupted basement membrane are lining the marginal zone sinus, which is surrounded by the diffuse lymphoid tissue of the marginal zone, which is formed from different stages of maturation of: lymphocytes, plasma cells, dendritic cells, neutrophils, marginal zone macrophages, erythrocytes, platelets, and reticular cells, all of which are supported by a network of reticular fibers and smooth muscles. Motile lymphocytes with pseudopodia are present in the splenic marginal zone of the Barki sheep. Two forms of smooth muscles are also present: solitary and doubled reciprocal smooth muscles, which give the spleen a powerful ability to filter the blood and provide defense against foreign antigens, making it a unique intermediate type of spleen.
边缘区是脾脏白髓边缘区域的一个独特结构,作为一个短暂的区域,新到达的细胞、抗原和血液在这里被过滤。巴尔基羊脾缘区围绕白色髓质,存在于白色髓质和红色髓质之间。它由边缘区窦和弥漫性淋巴组织聚集组成,其中点缀着平滑肌细胞和红细胞,并由网状细胞支持。位于中断的基底膜上的杆状内皮细胞排列在边缘区窦内,边缘区窦被边缘区弥漫性淋巴组织包围,这些淋巴组织由不同成熟阶段形成:淋巴细胞、浆细胞、树突状细胞、中性粒细胞、边缘区巨噬细胞、红细胞、血小板和网状细胞,所有这些细胞都由网状纤维和平滑肌网络支持。巴尔基羊脾缘区可见假足运动淋巴细胞。两种形式的平滑肌也存在:单反和双反平滑肌,它们赋予脾脏强大的过滤血液和防御外来抗原的能力,使其成为一种独特的中间类型的脾脏。
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引用次数: 0
Detection of Enterococcus faecalis as an indicator organism in abattoirs´ environment as well as meat 粪肠球菌在屠宰场环境和肉类中作为指示生物的检测
Pub Date : 2022-12-22 DOI: 10.21608/mjvm.2022.180284.1014
Ibrahim Rabah, Mohammad A. Nossair, A. Mansour, F. Soliman, Nehad A. Saleh
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引用次数: 0
Hepatoprotective Effect of Quercetin Nanoparticles Against Fipronil Toxicity in Adult Male Rats 槲皮素纳米颗粒抗氟虫腈对成年雄性大鼠的肝保护作用
Pub Date : 2022-04-11 DOI: 10.21608/mjvm.2022.127917.1010
M. Behery, Y. El-Sayed, M. Eldakroury, E. Abdelhiee
Fipronil (FPN), a phenyl pyrazole insecticide, has gotten a lot of attention recently because of its toxicity in mammals, mostly mediated by its effects on the antioxidant system. Quercetin is a flavonoid present in many fruits and vegetables that have been demonstrated to have a variety of beneficial biological effects, including anti-inflammatory and antioxidant properties. Nanoparticles of quercetin have been shown to improve its solubility and stability, allowing for improved absorption, cellular absorption, and lower toxicity. The present study was undertaken to explore the prophylactic effect of quercetin nanoparticles (QueNPs) against sub-chronic FPN-induced hepatic injury and oxidative stress in male rats. Experimental animals were orally gavaged for four weeks. It has been shown that FPN elevated the sera of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) whereas serum total protein level was decreased. FIP also caused histological changes in hepatic tissue. The QueNPs counteracted the hepatotoxic effect of FPN exposure.
氟虫腈(Fipronil, FPN)是一种苯基吡唑类杀虫剂,近年来因其对哺乳动物的毒性而受到广泛关注,其毒性主要是通过对抗氧化系统的影响来介导的。槲皮素是一种黄酮类化合物,存在于许多水果和蔬菜中,已被证明具有多种有益的生物效应,包括抗炎和抗氧化特性。槲皮素纳米颗粒已被证明可以改善其溶解度和稳定性,从而提高吸收、细胞吸收和降低毒性。本研究旨在探讨槲皮素纳米颗粒(QueNPs)对亚慢性fpn诱导的雄性大鼠肝损伤和氧化应激的预防作用。实验动物灌胃4周。结果表明,FPN提高了血清中谷丙转氨酶(ALT)和天冬氨酸转氨酶(AST),降低了血清总蛋白水平。FIP还引起肝组织的组织学改变。QueNPs抵消了FPN暴露的肝毒性作用。
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引用次数: 0
New Insights into the Splenic Stroma of the Barki Sheep: A Histological, Immunohistochemical, Ultrastructural and Morphometrical Study 巴基羊脾基质的组织学、免疫组织化学、超微结构和形态计量学研究
Pub Date : 2022-04-09 DOI: 10.21608/mjvm.2022.111483.1008
Omnya Elhussieny, E. Khalifa, Mohamed O. Abdelaziz
Introduction: Spleen is the largest secondary lymphatic organ in the body designed to respond immunologically to antigens circulating in the blood. Aim: describe the histoarchitectural features and morphometrical characters of the stroma of the Barki sheep’s spleen. Materials and Methods: the splenic stroma of 30 clinically healthy Barki sheep was studied using different histological, immunohistochemical, ultrastructural, and morphometrical techniques. Results: A prominent layer of smooth muscles with few sporadic collagen fibers form the capsule which was 676.29 μm and has a subcapsular sinus. Meanwhile, the trabeculae which were composed mainly of smooth muscle cells originate from the capsule into the splenic parenchyma branch into primary (517.095 μm thick), secondary (470.609 μm thick), and tertiary trabeculae (382.27 μm thick). The subcapsular blood sinuses and the peritrabecular blood sinuses all are connected and are engorged with blood cells mainly CD3+ T lymphocytes. Conclusion: the high amount of smooth muscles and very thick stroma is unique to Barki sheep’s spleen denoting an efficient storage function beside the defensive function. CD3 T lymphocytes in subcapsular and peritrabecular sinuses are to form a link between primed T lymphocytes and the underlying stromal elements, creating microenvironments suitable for effector responses.
脾脏是人体最大的次级淋巴器官,对血液中循环的抗原产生免疫反应。目的:描述巴基羊脾脏基质的组织结构特征和形态计量学特征。材料与方法:采用不同的组织学、免疫组织化学、超微结构和形态计量学技术对30只临床健康的巴基羊脾间质进行研究。结果:囊膜呈平滑肌层,直径676.29 μm,有少量散发性胶原纤维,囊下有窦。小梁主要由平滑肌细胞组成,由被膜向脾实质分支分化为初级小梁(517.095 μm)、次级小梁(470.609 μm)和三级小梁(382.27 μm)。包膜下血窦和小梁周围血窦连通,充血细胞以CD3+ T淋巴细胞为主。结论:巴尔基羊脾脏具有大量的平滑肌和很厚的间质,这是巴尔基羊脾脏所特有的,除了防御功能外,还具有有效的储存功能。包膜下和小梁周围窦的CD3 T淋巴细胞在启动T淋巴细胞和底层基质元素之间形成联系,创造适合效应反应的微环境。
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引用次数: 0
Effect of aflatoxin on kidney pathology in broilers 黄曲霉毒素对肉鸡肾脏病理的影响
Pub Date : 2022-04-09 DOI: 10.21608/mjvm.2022.122711.1009
Aya Ashry, Nabil M. Taha, M. Lebda, S. Ezzat, M. Elkamshishi
Aflatoxin (AFB1) causes toxic effects and leads to organ damage in broilers. This study was conducted to evaluate the pathological effect of aflatoxin B1 on the kidneys of broilers and mitigate its effect by using Saccharomyces yeast cell walls (YCW) extract and nano curcumin (NC) as dietary supplements. A total of 150 one-day-old, unsexed Cobb broiler chicks were allocated into 5 groups. The experimental feeding was designed as follows: Group A: control diet, group B: control diet with 0.25 mg/kg aflatoxin (AFB1), group C: control diet with 0.25 mg/kg AFB1+ YCW 1 kg/ton, group D: control diet with 0.25 mg/kg AFB1+ NC 400 mg/kg, and group E: control diet with 0.25 mg/kg AFB1+ YCW 1 kg/ton + NC 200 mg/kg. Histopathology of the broiler kidneys was shown. Group A showed normal histology, whereas GB showed marked degenerative, necrotic, and desquamative of the renal tubular epithelium, glomerulus, and renal tubule. On the other side, GC, GD, and GE showed normal histology in which the nephron consists of the glomerulus and the renal tubule. The addition of YCW extract and nano curcumin can be an alternative to counteract the negative effect of AFB1 in broiler diets.
黄曲霉毒素(AFB1)可引起肉鸡的毒性作用并导致器官损伤。本试验旨在评价黄曲霉毒素B1对肉仔鸡肾脏的病理影响,并通过添加酵母细胞壁提取物和纳米姜黄素(NC)来减轻黄曲霉毒素B1对肉仔鸡肾脏的影响。选取150只1日龄无性别科布肉鸡,随机分为5组。试验设计如下:A组为对照饲粮,B组为对照饲粮添加0.25 mg/kg黄曲霉毒素(AFB1), C组为对照饲粮添加0.25 mg/kg AFB1+ YCW 1 kg/ kg, D组为对照饲粮添加0.25 mg/kg AFB1+ NC 400 mg/kg, E组为对照饲粮添加0.25 mg/kg AFB1+ YCW 1 kg/t + NC 200 mg/kg。显示了肉鸡肾脏的组织病理学。A组组织学正常,而GB组肾小管上皮、肾小球、肾小管明显退行、坏死、脱屑。另一方面,GC、GD、GE示正常组织学,肾元由肾小球和肾小管组成。在肉鸡饲粮中添加YCW提取物和纳米姜黄素可以抵消AFB1的负面影响。
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引用次数: 1
Ultrastructure comparison of the mouth opening between two marine fishes with different feeding habits (Red porgy and Boops boops fish) 两种不同食性的海鱼(红鲷鱼和Boops Boops鱼)开口的超微结构比较
Pub Date : 2022-04-09 DOI: 10.21608/mjvm.2021.103489.1007
M. Seif, M. Alsafy, Samir Elgendy, Mohamed El-Beskawy, M. Eldakroury
The present investigation aimed to describe two types of marine fish of different eating behavior (Carnivorous and omnivorous). The samples were collected from two different places, the Red porgy fishes were collected from the Red Sea while the Boops boops fishes were collected from the Mediterranean Sea. The total the Red porgy 20:23 while in Boops boops fishes were 17:18 cm. gross morphology the porgy was very thin. By SEM, the Red porgy upper lip was surrounded by different size teeth, it showed very striated and corrugated surface with different lines in its surface while the lower lip appeared with a cilia- like structure with different tall length and its apex somewhat thinner than its base. Boops boops fish upper lip appeared as a thick round structure with multiple prominent bumps which look-like small bushes gets out along the surface while its lower lip appeared very close to the teeth, it narrow anteriorly and increased in width posteriorly.
本研究旨在描述两种不同进食行为的海鱼(食肉和杂食性)。这些样本来自两个不同的地方,红porgy鱼是从红海采集的,而Boops Boops鱼是从地中海采集的。红鲷鱼的总长度为20:23,而Boops Boops鱼的总长度为17:18厘米。大体形态上,孔隙非常薄。扫描电镜观察,红多孔的上唇被大小不等的牙齿所包围,表面呈条纹状波纹状,表面有不同的纹路;下唇呈纤毛状结构,长度不等,顶端比底部略薄。Boops Boops鱼的上唇呈厚实的圆形结构,表面有多个突出的凸起,看起来像小灌木,下唇非常靠近牙齿,前窄后宽。
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引用次数: 1
Characterization of Shiga Toxin Escherichia coli O157: H7 from raw camel milk, Goat Meat and Minced Goat Meat sold at Local Markets in Matrouh Governorate. 从Matrouh省当地市场销售的生骆驼奶、山羊肉和山羊肉中提取的志贺毒素大肠杆菌O157: H7的鉴定
Pub Date : 2022-04-09 DOI: 10.21608/mjvm.2021.58520.1003
E. Elghazaly, E. Khalifa, S. Khaliel, H. Torky
Escherichia coli O 157 :H 7 is a major food borne pathogens of concern worldwide so, the present study was carried out for isolation of E. coli O 157 :H 7 , molecular characterization, detection of virulence genes, antibiogram and phenotypic detection of Extended spectrum beta lactamase (ESBL) producers among isolated strains from raw camel milk goat meat and minced goat meat. A total of 66 samples (44 raw camel milk, 15 goat meat and 7 minced goat meat) were collected from local retail outlets, butcher's shop and super markets in Matrouh Governorate. Samples were subjected to bacterial isolation and identification using cultural, biochemical, PCR, antibiogram and phenotypic detection of ESBL. Out of 66 samples, 7 (10.6%) E. coli O 157 :H 7 isolates were recovered. All 7 (100%) tested isolates were positive for flic H7 gene. 4 (57.14%) isolates were positive for stx 1 but, none of the isolates were positive for stx 2 gene. E. coli O 157 :H 7 isolates showed susceptibility to gentamycin (28.6%), chloramphenicol (57.1%), cefepime and cefuroxime (14.3%) while intermediate susceptible to nalidixic acid (71.4%), streptomycin (57.1%), chloramphenicol, ampicillin and cefepime (14.3%), but resistant to cefotaxime and ceftazidime (100%), ampicillin and cefuroxime (85.7%), gentamycin and cefepime (71.4%), streptomycin (42.9%), nalidixic acid and chloramphenicol (28.6%). only 14.3% of isolates were ESBL producers. Consequently, the goat meat is one of the major sources of E. coli O 157 :H 7 in Matrouh Governorate. Multiple antibiotic resistant profiles of E. coli O 157 :H 7 show a risk for public health and food safety as well as animal production so, proper antibiotics usage in animal production also needs attention. 5. Characterization of ESBLs according CLSI (2016): Isolates with reduced CTX and / CAZ was assessed using combination disc test An in the zone more than 5mm for either antimicrobial agent tested in combination with clavulonic acid vs. the zone
大肠杆菌o157: h7是世界范围内关注的主要食源性致病菌,本研究对从生驼乳山羊肉和山羊肉中分离的大肠杆菌o157: h7进行了分离、分子鉴定、毒力基因检测、广谱β -内酰胺酶(ESBL)产生菌的抗生素谱和表型检测。从Matrouh省的当地零售店、肉店和超级市场共收集了66个样本(44个生驼奶、15个山羊肉和7个碎山羊肉)。采用培养、生化、PCR、抗生素谱和表型检测对样本进行细菌分离和鉴定。66份样品中检出7株(10.6%)大肠杆菌o157: h7分离株。7株(100%)分离株均为H7基因阳性。stx1基因阳性4株(57.14%),stx2基因无阳性。大肠杆菌o157: h7对庆大霉素(28.6%)、氯霉素(57.1%)、头孢吡肟和头孢呋辛(14.3%)敏感,对萘啶酸(71.4%)、链霉素(57.1%)、氯霉素、氨苄西林和头孢吡肟(14.3%)中等敏感,对头孢噻肟和头孢他啶(100%)、氨苄西林和头孢呋辛(85.7%)、庆大霉素和头孢吡肟(71.4%)、链霉素(42.9%)、萘啶酸和氯霉素(28.6%)耐药。只有14.3%的分离株是ESBL生产者。因此,山羊肉是马特鲁省大肠杆菌o157: h7的主要来源之一。大肠杆菌o157: h7的多重抗生素耐药谱显示出对公共卫生和食品安全以及动物生产的风险,因此,在动物生产中适当使用抗生素也需要引起注意。5. 根据CLSI(2016)对ESBLs的表征:使用联合圆盘试验对CTX和/ CAZ降低的分离株进行了评估。无论是与克拉维酸联合测试的抗菌剂,还是与该区域相比,在超过5mm的区域内进行了评估
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引用次数: 0
The Prevalence and Associated Infection Risk Factors of the Blood Protozoan Parasites among the Small Ruminants in the North Coast of Egypt 埃及北海岸小反刍动物血原虫寄生虫流行及相关感染危险因素
Pub Date : 2022-04-09 DOI: 10.21608/mjvm.2021.102556.1006
M. Abdelsalam, Mohamed Bessat, S. Fadl, Wael Felefel
Several factors are affecting the productivity of small ruminants, infectious diseases and their causative pathogens are one of the most detrimental factors. Blood protozoan parasites are pathogens affecting small ruminants worldwide, particularly Egypt. Thus, the current study was done for detecting the prevalence of the blood protozoan parasites among small ruminants in the North Coast of Egypt. This was done through collecting 366 blood samples from four districts at Matrouh governorate. Microscopic examination of the Giemsa-stained blood smears was done to reveal the overall infection rate. Results showed a total prevalence of 68.30% (Babesia ovis, Theileria ovis. and Anaplasma ovis.). Sheep recorded a higher prevalence of 84.8% than that of goat. Females recorded the highest infection rate with a highly significant association with infection in relation to the open breeding (X2=72.501, P. value=0.000). The same significant result was observed with the presence of ticks on the examined animals (X2 =46.711, P. value=0.000). When summed up, we can conclude that the lack of vector control in Matrouh governorate, associated with a bad housing of herd, ignorance of the proper animal management, and the open breeding system are the reasons for the widespread infection with blood protozoan parasites among small ruminants.
影响小反刍动物生产力的因素有很多,传染病及其致病病原体是最有害的因素之一。血液原生动物寄生虫是影响全世界,特别是埃及的小型反刍动物的病原体。因此,本研究旨在检测埃及北海岸小型反刍动物中血原虫寄生虫的流行情况。这是通过从Matrouh省的四个县收集366份血液样本来完成的。显微镜检查吉姆萨染色的血涂片,以显示总体感染率。结果显示:鸡巴贝斯虫、鸡伊勒菌总患病率为68.30%;和鹅无原体)。绵羊的患病率为84.8%,高于山羊。雌性感染率最高,与开放养殖的感染率呈极显著相关(X2=72.501, p值=0.000)。被检动物身上存在蜱虫也有同样显著的结果(X2 =46.711, p值=0.000)。综上所述,我们可以得出结论,Matrouh省缺乏病媒控制,畜群居住条件差,缺乏适当的动物管理,以及开放式饲养系统是导致小反刍动物广泛感染血原虫寄生虫的原因。
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引用次数: 0
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Matrouh Journal of Veterinary Medicine
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