Bacterial lipases are a highly versatile class of hydrolytic enzymes which catalyze the hydrolysis of triglycerol to glycerol and fatty acids. They play an important role in biotechnological and industrial processes, including oleo chemical, food, pharmaceutical detergent formulation, cosmetic, leather, textile, and paper industries applications. Tremendous interest among scientists and industrialists has been arisen in the last decade in microbial lipases due to their versatility and ease of mass production. In the current work, natural oils such as Triglyceride, Tween 20, Tween 80, Olive oil, Sweet almond, Celery oil, Sesame, Rosemary oils were implemented to enhance lipase production by soil and pathogenic bacterial isolates. Results revealed that triglyceride and tween 80 had the best enhancing activity for lipase production in most isolates. It was also shown that Pseudomonas and Bacillus were the most potential lipase producing bacteria. A novel modified tributary agar was prepared by the addition of Sudan blue dye and used for the detection of lipase producers. It was shown to give a very clear zone even in the case of weak producing strains as compared to the original medium. Global Proceedings Repository American Research Foundation
{"title":"Detection of Some Types of Oils That Induce Lipase Production in Some Bacterial Isolates","authors":"Ashwaq Hazem Najem, Intithar Thanoon Ahmad","doi":"10.24897/acn.64.68.6015","DOIUrl":"https://doi.org/10.24897/acn.64.68.6015","url":null,"abstract":"Bacterial lipases are a highly versatile class of hydrolytic enzymes which catalyze the hydrolysis of triglycerol to glycerol and fatty acids. They play an important role in biotechnological and industrial processes, including oleo chemical, food, pharmaceutical detergent formulation, cosmetic, leather, textile, and paper industries applications. Tremendous interest among scientists and industrialists has been arisen in the last decade in microbial lipases due to their versatility and ease of mass production. In the current work, natural oils such as Triglyceride, Tween 20, Tween 80, Olive oil, Sweet almond, Celery oil, Sesame, Rosemary oils were implemented to enhance lipase production by soil and pathogenic bacterial isolates. Results revealed that triglyceride and tween 80 had the best enhancing activity for lipase production in most isolates. It was also shown that Pseudomonas and Bacillus were the most potential lipase producing bacteria. A novel modified tributary agar was prepared by the addition of Sudan blue dye and used for the detection of lipase producers. It was shown to give a very clear zone even in the case of weak producing strains as compared to the original medium. Global Proceedings Repository American Research Foundation","PeriodicalId":129850,"journal":{"name":"وقائع الملتقي العلمي الدولي المعاصر","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114139594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
يعتبر العقل أداة للتفكّر ، ولولا وجوده في الإنسان لما استطاع أن يتفكر في آيات الله سواء آياته القرآنية أو آياته الكونية، لذا جعل القرآن الكريم العقل مناط التكليف، فلم يكلّف المجنون أو القاصر الذي لم يبلغ سنَّ التمييز، وجاء خطابه للعقلاء فقط، فقال تعالى: ﴿أَتَأْمُرُونَ النَّاسَ بِالْبِرِّ وَتَنسَوْنَ أَنفُسَكُمْ وَأَنتُمْ تَتْلُونَ الْكِتَابَ أَفَلاَ تَعْقِلُونَ﴾]البقرة: 44[، وقال أيضا ﴿يُؤتِي الْحِكْمَةَ مَن يَشَاءُ وَمَن يُؤْتَ الْحِكْمَةَ فَقَدْ أُوتِيَ خَيْراً كَثِيراً وَمَا يَذَّكَّرُ إِلاَّ أُوْلُواْ الأَلْبَابِ ﴾]البقرة: 269[ ، وقد أودع الله تعالى في القرآن الكريم منهجا ربانياً للاستدلال والتفكر يقوم على إعمال العقل وتحريم تعطيله أو استخدامه في ما يضرّ الناس ، لذا عُدت إلى كتاب الله تعالى لأبحث عن هذا المنهج الرباني ، مستعيناً بأمهات كتب التفاسير وبالكتب ذات العلاقة بالعقل والتفكر.
{"title":"المنهج القرآني في تغيير الإنسان","authors":"موسى محمود طه معطان","doi":"10.24897/acn.64.68.6038","DOIUrl":"https://doi.org/10.24897/acn.64.68.6038","url":null,"abstract":"يعتبر العقل أداة للتفكّر ، ولولا وجوده في الإنسان لما استطاع أن يتفكر في آيات الله سواء آياته القرآنية أو آياته الكونية، لذا جعل القرآن الكريم العقل مناط التكليف، فلم يكلّف المجنون أو القاصر الذي لم يبلغ سنَّ التمييز، وجاء خطابه للعقلاء فقط، فقال تعالى: ﴿أَتَأْمُرُونَ النَّاسَ بِالْبِرِّ وَتَنسَوْنَ أَنفُسَكُمْ وَأَنتُمْ تَتْلُونَ الْكِتَابَ أَفَلاَ تَعْقِلُونَ﴾]البقرة: 44[، وقال أيضا ﴿يُؤتِي الْحِكْمَةَ مَن يَشَاءُ وَمَن يُؤْتَ الْحِكْمَةَ فَقَدْ أُوتِيَ خَيْراً كَثِيراً وَمَا يَذَّكَّرُ إِلاَّ أُوْلُواْ الأَلْبَابِ ﴾]البقرة: 269[ ، وقد أودع الله تعالى في القرآن الكريم منهجا ربانياً للاستدلال والتفكر يقوم على إعمال العقل وتحريم تعطيله أو استخدامه في ما يضرّ الناس ، لذا عُدت إلى كتاب الله تعالى لأبحث عن هذا المنهج الرباني ، مستعيناً بأمهات كتب التفاسير وبالكتب ذات العلاقة بالعقل والتفكر.","PeriodicalId":129850,"journal":{"name":"وقائع الملتقي العلمي الدولي المعاصر","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129496409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}