Pub Date : 2020-10-19DOI: 10.23888/pavlovj2020283360-370
S. Kotlyarov, A. Kotlyarova
Despite all achievements of the modern medicine, the problem of chronic obstructive pulmonary disease (COPD) does not lose its relevance. The current paradigm suggests a key role of macrophages in inflammation in COPD. Macrophages are known to be heterogeneous in their functions. This heterogeneity is determined by their immunometabolic profile and also by peculiarities of lipid homeostasis of cells. Aim . To analyze the role of the ABCA1 transporter, a member of the ABC A subfamily, in the pathogenesis of COPD. The expression of ABCA1 in lung tissues is on the second place after the liver, which shows the important role of the carrier and of lipid homeostasis in the function of lungs. Analysis of the literature shows that participation of the transporter in inflammation consists in regulation of the content of cholesterol in the «lipid rafts» of the membranes, in phagocytosis and apoptosis. Conclusion . Through regulation of the process of reverse transport of cholesterol in macrophages of lungs, ABCA1 can change their inflammatory response, which makes a significant contribution to the pathogenesis of COPD.
{"title":"Participation of ABCA1 transporter in development of chronic obstructive pulmonary disease","authors":"S. Kotlyarov, A. Kotlyarova","doi":"10.23888/pavlovj2020283360-370","DOIUrl":"https://doi.org/10.23888/pavlovj2020283360-370","url":null,"abstract":"Despite all achievements of the modern medicine, the problem of chronic obstructive pulmonary disease (COPD) does not lose its relevance. The current paradigm suggests a key role of macrophages in inflammation in COPD. Macrophages are known to be heterogeneous in their functions. This heterogeneity is determined by their immunometabolic profile and also by peculiarities of lipid homeostasis of cells. Aim . To analyze the role of the ABCA1 transporter, a member of the ABC A subfamily, in the pathogenesis of COPD. The expression of ABCA1 in lung tissues is on the second place after the liver, which shows the important role of the carrier and of lipid homeostasis in the function of lungs. Analysis of the literature shows that participation of the transporter in inflammation consists in regulation of the content of cholesterol in the «lipid rafts» of the membranes, in phagocytosis and apoptosis. Conclusion . Through regulation of the process of reverse transport of cholesterol in macrophages of lungs, ABCA1 can change their inflammatory response, which makes a significant contribution to the pathogenesis of COPD.","PeriodicalId":13184,"journal":{"name":"I.P.Pavlov Russian Medical Biological Herald","volume":"64 1","pages":"360-370"},"PeriodicalIF":0.0,"publicationDate":"2020-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74163496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-10-19DOI: 10.23888/pavlovj2020283283-289
T. Bolobonkina, A. Dementyev, N. V. Shatrova, S. V. Yankina
Aim . Determination of the actual spectrum of pathogenic microorganisms to which medical workers of mobile teams of emergency medical care (EMC) are exposed, and scientific justification of the class of the working conditions of this professional group in terms of the biological factor. Materials and Methods. Data of the electronic journal of the automated «Ambulance Service» control system for infectious patients, and records of the register for infectious diseases among workers of the municipal emergency medical care station of Ryazan (300 medical assistants, 64 doctors) for 2016-2019 were used. Results. The average amount of calls to patients with infectious pathology in 2016-2019 made 2178.5±221.3 per year. Most emergency calls were for intestinal infections, common cases were varicella, erysipelas, viral hepatites, tuberculosis infection, herpes zoster. The main load in servicing these calls fell on linear (from 63.7 to 77.4%) and pediatric (from 24.8 to 35.1%) teams; calls of resuscitation teams to patients of such profile were sporadic. The level of infectious morbidity of doctors made 0.78 cases per 100 doctors which is 1.2-fold higher than the level of medical assistants (t=3.79; р=0.0003). Conclusion. The confirmed cases of diseases of the studied group of workers resulting from professional contacts with sources of infections of Group III pathogenicity permits to refer the working conditions of medical workers of mobile teams of emergency medical care stations to hazard Class II. However, a high probability for professional contact with high pathogenicity strains of group A influenza, HIV-1 and HIV-2, hepatitis C, D, E viruses belonging to Group II pathogenicity, and existence of the evident contacts with SARS-CoV-2, permit to raise the class of the working conditions to hazard Class III.
{"title":"Factors of biological nature in work of mobile teams of emergency medical care station on the eve of pandemics of new coronavirus infection (COVID-19)","authors":"T. Bolobonkina, A. Dementyev, N. V. Shatrova, S. V. Yankina","doi":"10.23888/pavlovj2020283283-289","DOIUrl":"https://doi.org/10.23888/pavlovj2020283283-289","url":null,"abstract":"Aim . Determination of the actual spectrum of pathogenic microorganisms to which medical workers of mobile teams of emergency medical care (EMC) are exposed, and scientific justification of the class of the working conditions of this professional group in terms of the biological factor. Materials and Methods. Data of the electronic journal of the automated «Ambulance Service» control system for infectious patients, and records of the register for infectious diseases among workers of the municipal emergency medical care station of Ryazan (300 medical assistants, 64 doctors) for 2016-2019 were used. Results. The average amount of calls to patients with infectious pathology in 2016-2019 made 2178.5±221.3 per year. Most emergency calls were for intestinal infections, common cases were varicella, erysipelas, viral hepatites, tuberculosis infection, herpes zoster. The main load in servicing these calls fell on linear (from 63.7 to 77.4%) and pediatric (from 24.8 to 35.1%) teams; calls of resuscitation teams to patients of such profile were sporadic. The level of infectious morbidity of doctors made 0.78 cases per 100 doctors which is 1.2-fold higher than the level of medical assistants (t=3.79; р=0.0003). Conclusion. The confirmed cases of diseases of the studied group of workers resulting from professional contacts with sources of infections of Group III pathogenicity permits to refer the working conditions of medical workers of mobile teams of emergency medical care stations to hazard Class II. However, a high probability for professional contact with high pathogenicity strains of group A influenza, HIV-1 and HIV-2, hepatitis C, D, E viruses belonging to Group II pathogenicity, and existence of the evident contacts with SARS-CoV-2, permit to raise the class of the working conditions to hazard Class III.","PeriodicalId":13184,"journal":{"name":"I.P.Pavlov Russian Medical Biological Herald","volume":"2 1","pages":"283-289"},"PeriodicalIF":0.0,"publicationDate":"2020-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83432839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-10-19DOI: 10.23888/pavlovj2020283350-359
R. Kalinin, I. Suchkov, Malay D. Patel, I. Shanaev, N. Mzhavanadze
Varicose disease is the most prevalent vascular disorder affecting lower extremities. Chronic venous insufficiency (CVI) is common in subjects with incompetent superficial and perforator veins. Major attention in pathogenesis of CVI is paid to horizontal venous reflux, while pathological blood flow in the superficial veins may sometimes be regarded as a postural reaction. At the same time cardiac pathology may also attribute to the development of CVI. The article presents a case report describing a female patient with combination of the right heart pathology and varicose disease associated with tricuspid regurgitation leading to constant venous reflux in the lower extremity superficial veins with further development of trophic changes.
{"title":"Combination of the right heart pathology and varicose disease as a factor of development of trophic changes in the lower extremities: a case report","authors":"R. Kalinin, I. Suchkov, Malay D. Patel, I. Shanaev, N. Mzhavanadze","doi":"10.23888/pavlovj2020283350-359","DOIUrl":"https://doi.org/10.23888/pavlovj2020283350-359","url":null,"abstract":"Varicose disease is the most prevalent vascular disorder affecting lower extremities. Chronic venous insufficiency (CVI) is common in subjects with incompetent superficial and perforator veins. Major attention in pathogenesis of CVI is paid to horizontal venous reflux, while pathological blood flow in the superficial veins may sometimes be regarded as a postural reaction. At the same time cardiac pathology may also attribute to the development of CVI. The article presents a case report describing a female patient with combination of the right heart pathology and varicose disease associated with tricuspid regurgitation leading to constant venous reflux in the lower extremity superficial veins with further development of trophic changes.","PeriodicalId":13184,"journal":{"name":"I.P.Pavlov Russian Medical Biological Herald","volume":"26 1","pages":"350-359"},"PeriodicalIF":0.0,"publicationDate":"2020-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74699181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-10-19DOI: 10.23888/pavlovj2020283371-376
M. A. Kiselev, N. B. Repina
Endometriosis is a chronic progressing estrogen-dependent disease with a high incidence among females characterized by pelvic pain (40-80%) and infertility (25-80%). Aim. To analyze and estimate the modern literature data on the possibility of using potential biomarkers of endometriosis in its non-invasive diagnostics. In recent years, researchers have made significant advances in understanding the disease-specific molecular pathways that regulate the development of ectopic foci of endometriosis, by examining the blood, peritoneal fluid, and eutopic endometrium in women with the disease. Along with understanding the pathophysiology of endometriosis, the question of finding an adequate biomarker that will provide an effective early non-invasive diagnosis of endometriosis and, accordingly, preserve the reproductive health of millions of women, remains relevant. Conclusion. Most reasonable in diagnostics of endometriosis is a combination of different biomarkers that also minimizes false positive and negative results in differential diagnosis.
{"title":"Non-invasive diagnostics of endometriosis: review of modern biomarkers of peripheral blood and endometrium","authors":"M. A. Kiselev, N. B. Repina","doi":"10.23888/pavlovj2020283371-376","DOIUrl":"https://doi.org/10.23888/pavlovj2020283371-376","url":null,"abstract":"Endometriosis is a chronic progressing estrogen-dependent disease with a high incidence among females characterized by pelvic pain (40-80%) and infertility (25-80%). Aim. To analyze and estimate the modern literature data on the possibility of using potential biomarkers of endometriosis in its non-invasive diagnostics. In recent years, researchers have made significant advances in understanding the disease-specific molecular pathways that regulate the development of ectopic foci of endometriosis, by examining the blood, peritoneal fluid, and eutopic endometrium in women with the disease. Along with understanding the pathophysiology of endometriosis, the question of finding an adequate biomarker that will provide an effective early non-invasive diagnosis of endometriosis and, accordingly, preserve the reproductive health of millions of women, remains relevant. Conclusion. Most reasonable in diagnostics of endometriosis is a combination of different biomarkers that also minimizes false positive and negative results in differential diagnosis.","PeriodicalId":13184,"journal":{"name":"I.P.Pavlov Russian Medical Biological Herald","volume":"43 1","pages":"371-376"},"PeriodicalIF":0.0,"publicationDate":"2020-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82209824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-03DOI: 10.23888/pavlovj2020282153-163
S. Yakushin, N. N. Nikulina, E. Filippov, S. Seleznev, E. Lygina, M. B. Chernysheva
Aim . Analysis of the relevance of drug-induced bradycardia (DIB) as a medical and social problem, its main regularities and determination of the need for further study of this issue. Materials and Methods . The register study was performed on 01 Jan 2017-30 Jun 2018 (18 months) at the clinical base of the Ryazan Regional Clinical Cardiology Dispensary. Inclusion criteria were: 1) bradycardia/bradyarrhythmia syndrome with intake of at least one drug with a bradycardic effect (BCE), 2) signing Informed consent to the processing of personal and clinical data. No additional interventions were performed in the diagnosis or treatment of patients within the registry. Results . During 18 months, 191 patients (age 77.0 [69.0;82.0] years, 26.7% of men) were hospitalized with a verified diagnosis of DIB, which accounted for 52.6% of all cases of hospitalization for drug overdose. During the analyzed period, there was an increase in both the total number of drug overdoses (1.7 times, p 3 s (7.4%). Almost all (94.8%) the patients were hospitalized by ambulance, 40.7% – to the intensive care unit; 17.8% required pacemaker implantation; hospital mortality was 5.2%. More than half (54.5%) of hospitalized patients took ≥2 drugs with BCE, 15.7% – ≥3 and 3.14% – ≥4 (both in monotherapy and as a part of a combination): beta-blockers – 68.4%, antiarrhythmic preparations – 38.9%, digoxin – 25.8%, non-dihydropyridine calcium antagonists – 10.5%, I1-imidazoline receptor agonist – 9.5%, and other drugs with BCE – 7.4%. To analyze the cause of DIB, we used clinical data of 135 patients (age 77.0 [69.0;82.0] years, 20.7% of men), who could indicate the exact dose of a taken drug with BCE. Among them, the absolute exceedance of the recommended dose of drugs with BCE was found in 14.1% of cases, while in 85.9% of cases summation/potentiation effect of several drugs with ВСЕ was observed, with intake of each in a therapeutic dose. Conclusion . The study confirmed high medical and social significance of the problem of DIB, which requires attention of practitioners, pharmacologists and clinical pharmacologists, health care providers, and also continuation of its study.
{"title":"Results of the pilot part of the cardiac drug overdoses hospital registry (storm): focus on drug-induced bradycardia","authors":"S. Yakushin, N. N. Nikulina, E. Filippov, S. Seleznev, E. Lygina, M. B. Chernysheva","doi":"10.23888/pavlovj2020282153-163","DOIUrl":"https://doi.org/10.23888/pavlovj2020282153-163","url":null,"abstract":"Aim . Analysis of the relevance of drug-induced bradycardia (DIB) as a medical and social problem, its main regularities and determination of the need for further study of this issue. Materials and Methods . The register study was performed on 01 Jan 2017-30 Jun 2018 (18 months) at the clinical base of the Ryazan Regional Clinical Cardiology Dispensary. Inclusion criteria were: 1) bradycardia/bradyarrhythmia syndrome with intake of at least one drug with a bradycardic effect (BCE), 2) signing Informed consent to the processing of personal and clinical data. No additional interventions were performed in the diagnosis or treatment of patients within the registry. Results . During 18 months, 191 patients (age 77.0 [69.0;82.0] years, 26.7% of men) were hospitalized with a verified diagnosis of DIB, which accounted for 52.6% of all cases of hospitalization for drug overdose. During the analyzed period, there was an increase in both the total number of drug overdoses (1.7 times, p 3 s (7.4%). Almost all (94.8%) the patients were hospitalized by ambulance, 40.7% – to the intensive care unit; 17.8% required pacemaker implantation; hospital mortality was 5.2%. More than half (54.5%) of hospitalized patients took ≥2 drugs with BCE, 15.7% – ≥3 and 3.14% – ≥4 (both in monotherapy and as a part of a combination): beta-blockers – 68.4%, antiarrhythmic preparations – 38.9%, digoxin – 25.8%, non-dihydropyridine calcium antagonists – 10.5%, I1-imidazoline receptor agonist – 9.5%, and other drugs with BCE – 7.4%. To analyze the cause of DIB, we used clinical data of 135 patients (age 77.0 [69.0;82.0] years, 20.7% of men), who could indicate the exact dose of a taken drug with BCE. Among them, the absolute exceedance of the recommended dose of drugs with BCE was found in 14.1% of cases, while in 85.9% of cases summation/potentiation effect of several drugs with ВСЕ was observed, with intake of each in a therapeutic dose. Conclusion . The study confirmed high medical and social significance of the problem of DIB, which requires attention of practitioners, pharmacologists and clinical pharmacologists, health care providers, and also continuation of its study.","PeriodicalId":13184,"journal":{"name":"I.P.Pavlov Russian Medical Biological Herald","volume":"13 1","pages":"153-163"},"PeriodicalIF":0.0,"publicationDate":"2020-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81509287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-03DOI: 10.23888/pavlovj2020282234-248
A. Artemenkov
Aim . Generalization of literature and proprietary data on genetic-physiological and evolutionary processes occurring in human populations in environmentally neglected industrial cities. In the review information is given about damage to the genetic apparatus of cells of a human organism under influence of unfavorable environmental factors and disadaptations of different genesis. To denote the totality of alterations induced by the given exposure, a new term is introduced – ‘genetics of disadaptations’. The information of mutagenic factors of the environment of industrial cities associated with growth of oncological diseases and of malformations resulting from chromosomal aberrations in cells is generalized. The problem of genetic burden of human populations in environmentally neglected territories and of the influence of disadaptive factors on this process is discussed. Information of the ecological situation and morbidity of the population in Cherepovets industrial city is given. A role of disadaptations in genetic-evolutionary processes occurring in human populations is shown. The cause of different manifestations of disadaptation in the population is stated to be divergence of traits. A hypothesis is proposed and evidences are given in favor of the existence of natural selection for a disadaptive trait in human populations. It is suggested that being accumulated in a human organism, disadaptive disorders may be transmitted to the next generations reducing vital ability of organisms and inducing different diseases. Conclusion . Within the topic, examples of different prophylactic measures for improving the health of the population of industrial cities are given to prevent unfavorable alterations of human genome under the influence of unfavorable ecological and related disadaptive factors.
{"title":"Disadaptive genetic-evolutionary processes in human populations of industrial cities","authors":"A. Artemenkov","doi":"10.23888/pavlovj2020282234-248","DOIUrl":"https://doi.org/10.23888/pavlovj2020282234-248","url":null,"abstract":"Aim . Generalization of literature and proprietary data on genetic-physiological and evolutionary processes occurring in human populations in environmentally neglected industrial cities. In the review information is given about damage to the genetic apparatus of cells of a human organism under influence of unfavorable environmental factors and disadaptations of different genesis. To denote the totality of alterations induced by the given exposure, a new term is introduced – ‘genetics of disadaptations’. The information of mutagenic factors of the environment of industrial cities associated with growth of oncological diseases and of malformations resulting from chromosomal aberrations in cells is generalized. The problem of genetic burden of human populations in environmentally neglected territories and of the influence of disadaptive factors on this process is discussed. Information of the ecological situation and morbidity of the population in Cherepovets industrial city is given. A role of disadaptations in genetic-evolutionary processes occurring in human populations is shown. The cause of different manifestations of disadaptation in the population is stated to be divergence of traits. A hypothesis is proposed and evidences are given in favor of the existence of natural selection for a disadaptive trait in human populations. It is suggested that being accumulated in a human organism, disadaptive disorders may be transmitted to the next generations reducing vital ability of organisms and inducing different diseases. Conclusion . Within the topic, examples of different prophylactic measures for improving the health of the population of industrial cities are given to prevent unfavorable alterations of human genome under the influence of unfavorable ecological and related disadaptive factors.","PeriodicalId":13184,"journal":{"name":"I.P.Pavlov Russian Medical Biological Herald","volume":"31 1","pages":"234-248"},"PeriodicalIF":0.0,"publicationDate":"2020-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82788532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-03DOI: 10.23888/pavlovj2020282127-134
E. P. Kulikov, A. I. Sudakov, A. Nikiforov, S. A. Mertsalov, V. A. Grigorenko
Aim. To determine the significance of polymorphism of MTHFR ( Ala 222 Val ), XPD ( Lis 751 Gln ), XRCC 1 ( Arg 194 Trp ), XRCC 1 ( Arg 399 Gln ), XRCC 1 ( Arg 208 His ), APE 1 ( Asp 148 Glu ), hOGG 1 ( ser 326 Ces ), P 53 ( Pro 47 Ser ), VEGF ( C 654 G ), EGFR ( A 2073 T ), TNF ( G 308 A ), CHEK 2 ( Ile 157 Thr ), MMP 1 (1607 1G>2G), TIMP 1( C 53 CT ) genes in development of colorectal cancer. Materials and Methods . 106 Cases of colorectal cancer in patients who were on treatment in Ryazan Clinical Oncological Dispensary (Ryazan) were analyzed. Genotyping in all patients was performed using the method of isolation of DNA from leukocytes of venous blood with subsequent polymerase chain reaction (PCR) with electrophoretic detection of the result. Results. No interrelation between the age of patients and polymorphism of any studied gene was recorded at the moment of verification of the diagnosis (р>0.05). Statistically significant relationship was identified between polymorphism of TNF ( G308A ) gene and the stage of cancer: its homozygous major genotype G/G more commonly occurred in the group of patients with III-IV stage (р=0.047). In the presence of allele of G/G TNF ( G308A ) gene together with homozygous mutant allele of MMP1 (1607 1G/2G ) gene , a direct relationship with increase in the number of patients diagnosed with III-IV stage was noted. This combination of two polymorphisms showed a statistically significant difference in the studied groups (р=0.025). In 8 out of 10 patients with IV stage, the presence of G/G polymorphism in VEGF ( C 654 G ) gene was noted. This mutant homozygous variant was much more rare in patents with I (37.5%), II (40%) or III stages (37.5%) (р=0.0147). Conclusions. The studied genes do not influence the age of manifestation of colorectal cancer and occur at the same frequency in patients of both genders irrespective of the age group. Localization and the extent of differentiation of the tumor do not depend on polymorphism of the studied genes either. The presence of G/A polymorphism of TNF (G308A) gene should be considered a favorable criterion associated with lower aggressiveness of the tumor (р<0.05), whereas identification of the major G/G genotype especially in combination with homozygous mutant allele of MMP1 (1607 1G/2G) gene is an unfavorable factor (р<0.05). The presence of G/G mutant genotype of VEGF ( C654 G) gene may directly correlate with rapid progression of tumor and with active metastatic spreading (р<0.05).
{"title":"Significance of gene polymorphysm in development of colorectal cancer","authors":"E. P. Kulikov, A. I. Sudakov, A. Nikiforov, S. A. Mertsalov, V. A. Grigorenko","doi":"10.23888/pavlovj2020282127-134","DOIUrl":"https://doi.org/10.23888/pavlovj2020282127-134","url":null,"abstract":"Aim. To determine the significance of polymorphism of MTHFR ( Ala 222 Val ), XPD ( Lis 751 Gln ), XRCC 1 ( Arg 194 Trp ), XRCC 1 ( Arg 399 Gln ), XRCC 1 ( Arg 208 His ), APE 1 ( Asp 148 Glu ), hOGG 1 ( ser 326 Ces ), P 53 ( Pro 47 Ser ), VEGF ( C 654 G ), EGFR ( A 2073 T ), TNF ( G 308 A ), CHEK 2 ( Ile 157 Thr ), MMP 1 (1607 1G>2G), TIMP 1( C 53 CT ) genes in development of colorectal cancer. Materials and Methods . 106 Cases of colorectal cancer in patients who were on treatment in Ryazan Clinical Oncological Dispensary (Ryazan) were analyzed. Genotyping in all patients was performed using the method of isolation of DNA from leukocytes of venous blood with subsequent polymerase chain reaction (PCR) with electrophoretic detection of the result. Results. No interrelation between the age of patients and polymorphism of any studied gene was recorded at the moment of verification of the diagnosis (р>0.05). Statistically significant relationship was identified between polymorphism of TNF ( G308A ) gene and the stage of cancer: its homozygous major genotype G/G more commonly occurred in the group of patients with III-IV stage (р=0.047). In the presence of allele of G/G TNF ( G308A ) gene together with homozygous mutant allele of MMP1 (1607 1G/2G ) gene , a direct relationship with increase in the number of patients diagnosed with III-IV stage was noted. This combination of two polymorphisms showed a statistically significant difference in the studied groups (р=0.025). In 8 out of 10 patients with IV stage, the presence of G/G polymorphism in VEGF ( C 654 G ) gene was noted. This mutant homozygous variant was much more rare in patents with I (37.5%), II (40%) or III stages (37.5%) (р=0.0147). Conclusions. The studied genes do not influence the age of manifestation of colorectal cancer and occur at the same frequency in patients of both genders irrespective of the age group. Localization and the extent of differentiation of the tumor do not depend on polymorphism of the studied genes either. The presence of G/A polymorphism of TNF (G308A) gene should be considered a favorable criterion associated with lower aggressiveness of the tumor (р<0.05), whereas identification of the major G/G genotype especially in combination with homozygous mutant allele of MMP1 (1607 1G/2G) gene is an unfavorable factor (р<0.05). The presence of G/G mutant genotype of VEGF ( C654 G) gene may directly correlate with rapid progression of tumor and with active metastatic spreading (р<0.05).","PeriodicalId":13184,"journal":{"name":"I.P.Pavlov Russian Medical Biological Herald","volume":"22 1","pages":"127-134"},"PeriodicalIF":0.0,"publicationDate":"2020-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74292057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-03DOI: 10.23888/pavlovj2020282183-192
R. Kalinin, I. Suchkov, N. Mzhavanadze, N. V. Korotkova, A. Nikiforov, I. Surov, P. Ivanova, A. D. Bozhenova, E. A. Strelnikova
Aim . To study and compare cytotoxicity of the main types of synthetic prostheses used in arterial reconstructive surgery, including polytetrafluoroethylene (PTFE) and polyethylene-terephthalate (Dacron). Materials and Methods . On the culture of human umbilical vein endothelial cells (HUVEC) of the 3 rd passage, MTS test was conducted that is used in laboratory examinations with attraction of cellular technologies to study cytotoxicity of medical drugs and medical products. The test implies use of MTS reagent that is 3-(4,5-dimethylthiazol-2-il)-5-(3-carboxymethoxyphenyl)-2- (4-sulfophenyl)-2H-tetrazolium; additionally phenazine methosulfate (PMS) was used that plays the role of electron-binding reagent. In the experiment, cells were incubated with PTFE and Dacron within 24 hours at 37ᵒC with 5% CO 2 . For control, HUVEC cultured in the standard growth medium, were used. In the presence of PMS, MTS was reduced by mitochondrial dehydrogenases of endothelial cells to formazan staining blue. Supernatant of cell cultures was evaluated by photocolorimetric method on Stat Fax 3200 analyzer (microplate reader) of Awareness technology Inc. Palm City Fl. (USA). Results . The lowest mean values were noted in Dacron group – 0.21 (0.20-0.22) optical density units, the highest values were noted in the control group – 0.36 (0.35-0.38); parameters in PTFE group were 0.35 (0.33-0.36). In comparison of the groups statistically significant differences were found between the control group and Dacron group (р<0.001), control and PTFE group (р=0.037), Dacron and PTFE (р<0.001). Incubation with Dacron led to suppression of metabolic activity of cells by 41.7% as compared to the control group (р<0.001). Metabolic activity of cells exposed to PTFE, approached that of the control group, that is, it corresponded to the optimal conditions of culturing of endothelial cells in vitro . Conclusion . In comparison with polyethylene-terephthalate (Dacron), polytetrafluoro-ethylene (PTFE) showed the least suppression of metabolic activity of endothelial cells in vitro .
{"title":"Comparison of cytotoxicity of vascular prostheses in vitro","authors":"R. Kalinin, I. Suchkov, N. Mzhavanadze, N. V. Korotkova, A. Nikiforov, I. Surov, P. Ivanova, A. D. Bozhenova, E. A. Strelnikova","doi":"10.23888/pavlovj2020282183-192","DOIUrl":"https://doi.org/10.23888/pavlovj2020282183-192","url":null,"abstract":"Aim . To study and compare cytotoxicity of the main types of synthetic prostheses used in arterial reconstructive surgery, including polytetrafluoroethylene (PTFE) and polyethylene-terephthalate (Dacron). Materials and Methods . On the culture of human umbilical vein endothelial cells (HUVEC) of the 3 rd passage, MTS test was conducted that is used in laboratory examinations with attraction of cellular technologies to study cytotoxicity of medical drugs and medical products. The test implies use of MTS reagent that is 3-(4,5-dimethylthiazol-2-il)-5-(3-carboxymethoxyphenyl)-2- (4-sulfophenyl)-2H-tetrazolium; additionally phenazine methosulfate (PMS) was used that plays the role of electron-binding reagent. In the experiment, cells were incubated with PTFE and Dacron within 24 hours at 37ᵒC with 5% CO 2 . For control, HUVEC cultured in the standard growth medium, were used. In the presence of PMS, MTS was reduced by mitochondrial dehydrogenases of endothelial cells to formazan staining blue. Supernatant of cell cultures was evaluated by photocolorimetric method on Stat Fax 3200 analyzer (microplate reader) of Awareness technology Inc. Palm City Fl. (USA). Results . The lowest mean values were noted in Dacron group – 0.21 (0.20-0.22) optical density units, the highest values were noted in the control group – 0.36 (0.35-0.38); parameters in PTFE group were 0.35 (0.33-0.36). In comparison of the groups statistically significant differences were found between the control group and Dacron group (р<0.001), control and PTFE group (р=0.037), Dacron and PTFE (р<0.001). Incubation with Dacron led to suppression of metabolic activity of cells by 41.7% as compared to the control group (р<0.001). Metabolic activity of cells exposed to PTFE, approached that of the control group, that is, it corresponded to the optimal conditions of culturing of endothelial cells in vitro . Conclusion . In comparison with polyethylene-terephthalate (Dacron), polytetrafluoro-ethylene (PTFE) showed the least suppression of metabolic activity of endothelial cells in vitro .","PeriodicalId":13184,"journal":{"name":"I.P.Pavlov Russian Medical Biological Herald","volume":"8 1","pages":"183-192"},"PeriodicalIF":0.0,"publicationDate":"2020-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91530469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-03DOI: 10.23888/pavlovj2020282164-170
A. Solodukhin, O. Trubnikova, O. Barbarash
Aim. Assessment and analysis of parameters of psychological status of patients with coronary heart disease (CHD) referred for coronary artery bypass surgery (CABG). Materials and Methods . A study of parameters of psychological status, in particular, of the attitude to illness, coping strategies, and lifelong orientations was conducted in 58 male patients aged 40 to 74 years with a stable form of CHD before and after CABG surgery. The parameters were evaluated by a clinical psychologist in 2-3 days after admission of the patient to the hospital and in 7-8 days after CABG. Clinical and psychological diagnostics was carried out using the following questionnaires: TOBOL (L.I. Wasserman, et al.), «Life-Purpose Orientations» (D.A. Leontiev), «Coping Behavior Strategies» (R. Lazarus, adapted version of T.A. Kryukova). Statistical analysis was performed with use of computer Statistica 10.0 software program. Results . After CABG patients with coronary artery disease show a significant reduction of the parameters of the anxious variant of the internal picture of the disease on the basis of TOBOL questionnaire, of the «positive re-evaluation» coping strategy on the basis of evaluation of stress-coping behavior and of the level of «Aim» scale on the basis of evaluation of the level of neurotization using the «Life-Purpose» test. Conclusion . The results of the study indicate reduction of the level of adaptation to the disease in patients with coronary artery disease after CABG in result of behavioral disorders and difficulties in setting further aims for recovery. To increase postoperative adaptation, the psychocorrection measures may be «targeted» to the coping strategy for «positive re-evaluation» and assistance in setting aims in the postoperative stage of CABG.
{"title":"Dynamics of parameters of psychological status of patients with stable ischemic heart disease and coronary artery bypass surgery","authors":"A. Solodukhin, O. Trubnikova, O. Barbarash","doi":"10.23888/pavlovj2020282164-170","DOIUrl":"https://doi.org/10.23888/pavlovj2020282164-170","url":null,"abstract":"Aim. Assessment and analysis of parameters of psychological status of patients with coronary heart disease (CHD) referred for coronary artery bypass surgery (CABG). Materials and Methods . A study of parameters of psychological status, in particular, of the attitude to illness, coping strategies, and lifelong orientations was conducted in 58 male patients aged 40 to 74 years with a stable form of CHD before and after CABG surgery. The parameters were evaluated by a clinical psychologist in 2-3 days after admission of the patient to the hospital and in 7-8 days after CABG. Clinical and psychological diagnostics was carried out using the following questionnaires: TOBOL (L.I. Wasserman, et al.), «Life-Purpose Orientations» (D.A. Leontiev), «Coping Behavior Strategies» (R. Lazarus, adapted version of T.A. Kryukova). Statistical analysis was performed with use of computer Statistica 10.0 software program. Results . After CABG patients with coronary artery disease show a significant reduction of the parameters of the anxious variant of the internal picture of the disease on the basis of TOBOL questionnaire, of the «positive re-evaluation» coping strategy on the basis of evaluation of stress-coping behavior and of the level of «Aim» scale on the basis of evaluation of the level of neurotization using the «Life-Purpose» test. Conclusion . The results of the study indicate reduction of the level of adaptation to the disease in patients with coronary artery disease after CABG in result of behavioral disorders and difficulties in setting further aims for recovery. To increase postoperative adaptation, the psychocorrection measures may be «targeted» to the coping strategy for «positive re-evaluation» and assistance in setting aims in the postoperative stage of CABG.","PeriodicalId":13184,"journal":{"name":"I.P.Pavlov Russian Medical Biological Herald","volume":"88 1","pages":"164-170"},"PeriodicalIF":0.0,"publicationDate":"2020-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74818934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-03DOI: 10.23888/pavlovj2020282193-199
V. A. Lipatov, D. A. Severinov, A. A. Denisov, S. V. Lazarenko, N. N. Grigor’yev
Taking into account the peculiarities of the blood supply to the liver and the structure of its parenchyma, and, as a result, considerable difficulties in providing reliable hemostasis without additional traumatization, a surgeon needs to be careful when performing a surgical manipulation. Despite a large number of modern methods for stopping parenchymal bleeding, many practi-tioners give preference to classic methods of hemostasis, including ligation of bleeding vessels in the bulk of the organ using specialized sutures. One of the most serious complications of the use of suture material is cutting of organ tissue, which leads to enhanced bleeding and expansion of the area of damage. At the moment, the tactics of choosing suture material for surgical procedures is empirical. In this context, the aim of this study was to assess the physico-mechanical properties of suture material regarding its structure, and to develop a criterion for choosing surgical sutures for surgical procedures. For the study, the liver was removed out of corpses of males of 35 to 40 years of age, after which separate sections of 7.5×7×4 cm in size were obtained from it. Then a wound 3 cm long and 2 cm deep was made. The wound was sutured with a simple interrupted suture without tightening, but with application of one simple half-node. For suturing, catgut, twisted capron and polyglycolide thread were used. Attention was paid to the maximal force applied at the moment of cutting (Fmax) and the degree of pulling the thread at the moment of cutting (Lu). The analysis of the data was based on comparison of Fmax and Lu parameters. This method permits to refuse from the empirical approach to the choice of suture material for operations on the liver, and to develop a criterion for choosing surgical sutures.
{"title":"Research of physical and mechanical characteristics of suture material in experiment in operations on liver","authors":"V. A. Lipatov, D. A. Severinov, A. A. Denisov, S. V. Lazarenko, N. N. Grigor’yev","doi":"10.23888/pavlovj2020282193-199","DOIUrl":"https://doi.org/10.23888/pavlovj2020282193-199","url":null,"abstract":"Taking into account the peculiarities of the blood supply to the liver and the structure of its parenchyma, and, as a result, considerable difficulties in providing reliable hemostasis without additional traumatization, a surgeon needs to be careful when performing a surgical manipulation. Despite a large number of modern methods for stopping parenchymal bleeding, many practi-tioners give preference to classic methods of hemostasis, including ligation of bleeding vessels in the bulk of the organ using specialized sutures. One of the most serious complications of the use of suture material is cutting of organ tissue, which leads to enhanced bleeding and expansion of the area of damage. At the moment, the tactics of choosing suture material for surgical procedures is empirical. In this context, the aim of this study was to assess the physico-mechanical properties of suture material regarding its structure, and to develop a criterion for choosing surgical sutures for surgical procedures. For the study, the liver was removed out of corpses of males of 35 to 40 years of age, after which separate sections of 7.5×7×4 cm in size were obtained from it. Then a wound 3 cm long and 2 cm deep was made. The wound was sutured with a simple interrupted suture without tightening, but with application of one simple half-node. For suturing, catgut, twisted capron and polyglycolide thread were used. Attention was paid to the maximal force applied at the moment of cutting (Fmax) and the degree of pulling the thread at the moment of cutting (Lu). The analysis of the data was based on comparison of Fmax and Lu parameters. This method permits to refuse from the empirical approach to the choice of suture material for operations on the liver, and to develop a criterion for choosing surgical sutures.","PeriodicalId":13184,"journal":{"name":"I.P.Pavlov Russian Medical Biological Herald","volume":"54 4 1","pages":"193-199"},"PeriodicalIF":0.0,"publicationDate":"2020-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76152290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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