Insect Biochemistry最新文献

Members of the adipokinetic hormone/red pigment-concentrating hormone (AKH/RPCH) family characteristically cause metabolite mobilization by the insect fat body. The present study identified several additional physiological actions in adult Blaberus discoidalis cockroaches that were influenced by synthetic Blaberus hypertrehalosemic hormone (HTH) and other AKH/RPCH family peptides. HTH elevated blood carbohydrate by 4-fold and cytochrome heme a + b synthesis of fat body mitochondria by 3-fold. Both carbohydrate and heme synthesis were dose-responsive to HTH. Carbohydrate synthesis was 10 times more sensitive to HTH than heme synthesis. Heme synthesis was also stimulated by Periplaneta cardioacceleratory hormones (CAH)-I and -II and RPCH but not by AKH-I or -II, at the doses tested. HTH showed strong cardioexcitatory activity. Long-term treatment of decapitated female B. discoidalis with juvenile hormone analog (JHA = methoprene) stimulated by 2.6-fold the rate of synthesis of secreted fat body proteins. HTH enhanced the JHA-dependent export protein synthesis by 42% above that observed with JHA alone. SDS-PAGE demonstrated that JHA determined the nature of the newly synthesized polypeptides; HTH enhanced their synthesis rate. Neither AKH-I nor HTH affected protein synthesis when added directly to isolated fat body. These results demonstrate that peptides of the AKH/RPCH family have multiple physiological actions related to fat body energy metabolism.

Unlike some moths, pheromone production in Trichoplusia ni is not regulated by a pheromone activating neuropeptide. Rather, competency to produce pheromone apparently is linked with changes in the ecdysteroid titer that occur late in metamorphosis. In contrast to adult pheromone glands, glands from pharate adults 2 days before eclosion were non-competent, and (1) had undetectable levels of the pheromone, (Z)-7-dodecenyl acetate, and pheromone-specific intermediates, (2) showed little or no conversion of radiolabeled substrate to product in enzyme assays of fatty acid synthetase, Δ11 desaturase, and acetyltransferase, and (3) failed to incorporate radiolabeled acetate into pheromone in gland culture. Glands 1 day before adult eclosion exhibited low titers of pheromone and the intermediate, (Z)-11-hexadecenoate, and showed low levels of radiolabeled acetate incorporation into pheromone in gland culture. By the time of adult eclosion, the gland was fully competent. Precocious development of pheromone gland competency was induced by removing the head and thorax from pupae 2 days before adult eclosion. This effect appears to result from the reduction of ecdysteroid, since it was blocked by the administration of 20-hydroxyecdysone. This ability to manipulate the development of the pheromone gland was restricted to a critical period, since removal of head and thorax from younger pupae did not induce pheromone gland competency, and administration of 20-hydroxyecdysone to older pupae did not block its onset. In addition to differences in competency, early pharate and adult glands exhibited dissimilarities with respect to (1) the types of proteins synthesized in gland culture, and (2) the types of proteins translated from mRNA in vitro.

Four closely related eclosion hormones (EHs), EH-I, -II, -III and -IV, were purified from 770,000 pharate adult heads of the silkworm, Bombyx mori. The complete amino acid sequence was determined by Edman degradation of peptide fragments generated by enzymatic digestion. Preliminary results have been reported (Kono et al., Agric. biol. Chem.51, 2307–2308, 1987). Accumulated data suggested that EH-IV has the longest sequence consisting of 62 residues. EH-I and -III have N-termini truncated by two residues. EH-I and -II terminate at position 61. The removal of the C-terminal leucine in EH-I and -II might have occurred artificially during extraction.

The isolated hindgut preparation of the cockroach, Leucophaea maderae has provided an effective bioassay tool for the isolation of certain structural types of insect myotropic peptides. Initially, the preparation was used to monitor excitatory and inhibitory activities of numerous HPLC fractions in a study that resulted in the structural characterization of 12 Leucophaea neuropeptides. Subsequently, the preparation was used as the bioassay for the isolation and structural characterization of myotropic neuropeptides of the house cricket, Acheta domesticus, and the locust, Locusta migratoria. Five novel myotropic peptides from the cricket were structurally characterized, and 32 separate myotropic compounds were isolated from nervous tissue of the locust. At present, 8 of the locust peptides have been structurally characterized. Isolation studies using this bioassay have been responsible for the discovery of 25 unique neuropeptides, 4 new peptide families, and the initial demonstration of the natural analog phenomenon in insects.

A sensitive assay for kynurenine transaminase activity (E.C. 2.6.1.7) based on rapid separation of the reaction product by high performance liquid chromatography (HPLC) has been developed. Drosophila sordidula extracts have been assayed by this new method and this is the first time that kynurenine transaminase activity has been demonstrated in Drosophila. The method of assay developed can be extended to any other organism. Kynurenine and 3-hydroxykynurenine were both used as substrates, and they were transaminated to kynurenic acid and xanthruenic acid, respectively. HPLC is used to separate and quantitate these reaction products from all other components in the reaction mixture.

In crude extracts from Drosophila, the reaction requires pyridoxal 5′-phosphate and an amino acid acceptor. The enzyme activity showed a maximum at 47°C and pH 8.0 with kynurenine and pyruvic acid as substrates. Transaminase activity was present in both head and body, nevertheless the specific activity was higher in the former. In bodies, pyruvic acid was the best amino acceptor whereas in heads it was α-oxoglutaric acid. The variation of kynurenine transaminase during development of D. sordidula showed, in the larval and pupal stages, activity levels practically constant and much lower than those found in the adult. This seems to suggest a preferential role of this enzyme in the metabolism of intermediates in the biosynthesis of ommochromes.

Recent developments in automated peptide microsequencing, liquid secondary-ion and electrospray mass spectrometry enable unambiguous primary structure determinations of minute amounts of biological material. We have used these methods in combination to characterize the predominant peptides from HPLC eluates of aqueous extracts of corpora cardiaca from adults of Locusta migratoria. Among the molecules or families of molecules clearly predominating in the extracts, we had previously characterized novel peptides (Hietter et al., 1989, 1990), and we recently identified three structurally-related, cysteine-rich, 8–9 kDa peptides. We present in this paper their complete structure determination. The amino acid sequence of these peptides is superimposable to that of neuroparsins isolated as dimers by Girardie et al., 1989. However, our experimental data lead us to propose that these molecules are monomers containing six intramolecular disulfide bridges.