The presence of artifacts in whole slide images (WSIs), such as tissue folds, air bubbles, and out-of-focus regions, can significantly impact WSI digitization, pathologists' evaluation, and the accuracy of downstream analyses. We present SlideInspect, a novel AI-based framework for comprehensive artifact detection and quality control in digital pathology. Our system leverages deep learning techniques to segment multiple artifact types across diverse tissue types and staining methods. SlideInspect provides a hierarchical output: a color-coded slide quality indicator (green, yellow, red) with recommended actions (no action, re-scan, re-mount, re-cut) based on artifact type and extent, and pixel-level segmentation masks for detailed analysis. The system operates at multiple magnifications (1.25× for tissue segmentation, 5× for artifact detection) and also incorporates stain quality assessment for histological stain evaluation. We validated SlideInspect on a large, multi-centric, multi-scanner dataset of over 3000 WSIs, demonstrating robust performance across different tissue types, staining methods, and scanning platforms. The system achieves high segmentation accuracy for various artifacts while maintaining computational efficiency (average processing time: 72.7 s per WSI). Pathologist evaluations confirmed the clinical relevance and accuracy of SlideInspect's quality assessments. By providing actionable insights at multiple levels of granularity, SlideInspect significantly improves the efficiency and standardization of digital pathology workflows. Its vendor-agnostic design and multi-stain capability make it suitable for integration into diverse clinical and research settings.
在整个幻灯片图像(WSI)中存在伪影,如组织褶皱、气泡和失焦区域,会严重影响WSI数字化、病理学家的评估和下游分析的准确性。我们提出了SlideInspect,一个新的基于人工智能的框架,用于数字病理学中全面的伪影检测和质量控制。我们的系统利用深度学习技术在不同的组织类型和染色方法中分割多种工件类型。SlideInspect提供了一个分层输出:一个颜色编码的幻灯片质量指示器(绿色,黄色,红色),根据工件类型和程度推荐操作(无操作,重新扫描,重新安装,重新切割),以及用于详细分析的像素级分割掩码。该系统可在多种倍率下工作(1.25倍用于组织分割,5倍用于伪影检测),并结合染色质量评估用于组织学染色评估。我们在超过3000个wsi的大型、多中心、多扫描仪数据集上验证了SlideInspect,展示了在不同组织类型、染色方法和扫描平台上的稳健性能。该系统在保持计算效率(平均处理时间:72.7 s / WSI)的同时,实现了对各种工件的高分割精度。病理学家的评估证实了SlideInspect质量评估的临床相关性和准确性。通过在多个粒度级别提供可操作的见解,SlideInspect显着提高了数字病理工作流程的效率和标准化。其供应商不可知的设计和多染色能力使其适合整合到不同的临床和研究设置。
{"title":"SlideInspect: From Pixel-Level Artifact Detection to Actionable Quality Metrics in Digital Pathology","authors":"Manuela Scotto, Roberta Patti, Vincenzo L'imperio, Filippo Fraggetta, Filippo Molinari, Massimo Salvi","doi":"10.1002/ima.70292","DOIUrl":"https://doi.org/10.1002/ima.70292","url":null,"abstract":"<p>The presence of artifacts in whole slide images (WSIs), such as tissue folds, air bubbles, and out-of-focus regions, can significantly impact WSI digitization, pathologists' evaluation, and the accuracy of downstream analyses. We present SlideInspect, a novel AI-based framework for comprehensive artifact detection and quality control in digital pathology. Our system leverages deep learning techniques to segment multiple artifact types across diverse tissue types and staining methods. SlideInspect provides a hierarchical output: a color-coded slide quality indicator (green, yellow, red) with recommended actions (no action, re-scan, re-mount, re-cut) based on artifact type and extent, and pixel-level segmentation masks for detailed analysis. The system operates at multiple magnifications (1.25× for tissue segmentation, 5× for artifact detection) and also incorporates stain quality assessment for histological stain evaluation. We validated SlideInspect on a large, multi-centric, multi-scanner dataset of over 3000 WSIs, demonstrating robust performance across different tissue types, staining methods, and scanning platforms. The system achieves high segmentation accuracy for various artifacts while maintaining computational efficiency (average processing time: 72.7 s per WSI). Pathologist evaluations confirmed the clinical relevance and accuracy of SlideInspect's quality assessments. By providing actionable insights at multiple levels of granularity, SlideInspect significantly improves the efficiency and standardization of digital pathology workflows. Its vendor-agnostic design and multi-stain capability make it suitable for integration into diverse clinical and research settings.</p>","PeriodicalId":14027,"journal":{"name":"International Journal of Imaging Systems and Technology","volume":"36 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ima.70292","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146002177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"计算机科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}