{"title":"Root Growth of Arbequina Cuttings as Influenced by Organic and Inorganic Substrates under the Conditions of Al-Jouf (KSA)","authors":"H. Mehri, K. Mhanna, A. Soltane","doi":"10.3923/AJPP.2013.74.83","DOIUrl":"https://doi.org/10.3923/AJPP.2013.74.83","url":null,"abstract":"","PeriodicalId":142158,"journal":{"name":"American Journal of Plant Physiology","volume":"36 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115836265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
PAUL CHIDOKA CHIKEZIE, A. R. Akuwudike, C. Chikezie, C. Ibegbulem
{"title":"Fractional Purification and Kinetic Parameters (Km and Vmax) of Polyphenol Oxidase Extracted from Three Segments of Solanum melongenas and Musa sapientum Fruits","authors":"PAUL CHIDOKA CHIKEZIE, A. R. Akuwudike, C. Chikezie, C. Ibegbulem","doi":"10.3923/AJPP.2013.84.92","DOIUrl":"https://doi.org/10.3923/AJPP.2013.84.92","url":null,"abstract":"","PeriodicalId":142158,"journal":{"name":"American Journal of Plant Physiology","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130614417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Isa, K. C. Egbuche, M. M. Malgwi, Nafisatu A. Tukur
{"title":"Cytological Studies in Eichhornia crassipes (Mart.) Solms","authors":"H. Isa, K. C. Egbuche, M. M. Malgwi, Nafisatu A. Tukur","doi":"10.3923/AJPP.2013.50.62","DOIUrl":"https://doi.org/10.3923/AJPP.2013.50.62","url":null,"abstract":"","PeriodicalId":142158,"journal":{"name":"American Journal of Plant Physiology","volume":"2016 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127497624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Enzyme activity depends largely on environmental conditions such as temperature and pH. The stability of polyphenol oxidase (PPO) extracted from Solanum melongenas and Musa sapientum fruits preincubated in varying thermal and pH conditions were carried out. Enzyme activity was measured by spectrophotometric methods. The reaction mixture contained 3.5 mL of 0.20 M phosphate buffer (pH = 6.8), 1.0 mL of 0.75 mM catechol, and 0.5 mL of enzyme solution. PPO S. melongenas and PPO M. sapientum gave different temperature and pH optima. The temperature-activity profile of PPO S. melongenas and PPO M. sapientum showed a strong positive correlation ( r = 0.907363). At pH = 10.0, PPO M. sapientum activity represented 65.3% decay in enzyme activity, whereas PPO S. melongenas represented 79.3% decay in enzyme activity. PPOS.melongenas and PPO M. sapientum stability at pre-incubated temperatures of 20, 50 and 60°C and pH values of 3.5, 6.0 and 8.0 were measured. Residual activities of PPO S.melongenas and PPO M. sapientum showed a strong positive correlations under the same experimental thermal conditions, with exception at 20°C ( r = 0.693375). Specifically, pre-incubation of PPO M.sapientum for t = 90 min at 60°C caused 18.4% decay in relative activity of PPO M. sapientum . At t = 90 min, pre-incubation of PPO M. sapientum , at pH = 3.5 caused decay in activity within the range of 30.8-36.1%, whereas PPO M. sapientum pre-incubated in pH = 6.0 and pH = 8.0 gave decay in activity within the range of (1.5-9.8%) and (2.7-6.5%) respectively. PPO S.melongenas and PPO M. sapientum showed relatively higher stabilities as the incubation pH tended towards alkaline conditions, whereas the two experimental temperatures (20 and 60°C) promoted destabilization. Keywords : Polyphenol oxidase, temperature, pH, Solanum melongenas and Musa sapientum . African Journal of Biotechnology Vol. 12(38), pp. 5688-5694
酶的活性在很大程度上取决于环境条件，如温度和pH。本文研究了在不同温度和pH条件下预孵育的龙葵和Musa sapientum果实中提取的多酚氧化酶(PPO)的稳定性。用分光光度法测定酶活性。反应混合物中含有3.5 mL 0.20 M磷酸盐缓冲液(pH = 6.8)， 1.0 mL 0.75 mM儿茶酚，0.5 mL酶溶液。PPO melongenas和PPO M. sapientum的最佳温度和pH值不同。PPO S. melongenas与PPO M. sapientum的温度-活性曲线呈极显著正相关(r = 0.907363)。pH = 10.0时，PPO M. sapientum酶活性下降65.3%，而PPO S. melongenas酶活性下降79.3%。ppo。在预孵育温度分别为20、50和60℃，pH分别为3.5、6.0和8.0时，测定了melongenas和PPO M. sapientum的稳定性。在相同的实验温度条件下，PPO S.melongenas和PPO m.s apientum的剩余活性除在20℃时呈显著正相关外(r = 0.693375)。具体来说，在60°C条件下，PPO M.sapientum预孵育90分钟，PPO M.sapientum的相对活性下降18.4%。pH = 3.5预孵育90 min时，PPO M. sapientum活性衰减幅度为30.8 ~ 36.1%，pH = 6.0和pH = 8.0预孵育90 min后，PPO M. sapientum活性衰减幅度分别为(1.5 ~ 9.8%)和(2.7 ~ 6.5%)。PPO s.m ongenas和PPO M. sapientum在培养pH趋于碱性时表现出较高的稳定性，而20℃和60℃两个实验温度促进了不稳定性。关键词:多酚氧化酶;温度;pH;非洲生物技术杂志Vol. 12(38)， pp. 5688-5694
{"title":"Thermal and pH Stabilities of Partially Purified Polyphenol Oxidase Extracted from Solanum melongenas and Musa sapientum Fruits","authors":"PAUL CHIDOKA CHIKEZIE","doi":"10.3923/AJPP.2013.63.73","DOIUrl":"https://doi.org/10.3923/AJPP.2013.63.73","url":null,"abstract":"Enzyme activity depends largely on environmental conditions such as temperature and pH. The stability of polyphenol oxidase (PPO) extracted from Solanum melongenas and Musa sapientum fruits preincubated in varying thermal and pH conditions were carried out. Enzyme activity was measured by spectrophotometric methods. The reaction mixture contained 3.5 mL of 0.20 M phosphate buffer (pH = 6.8), 1.0 mL of 0.75 mM catechol, and 0.5 mL of enzyme solution. PPO S. melongenas and PPO M. sapientum gave different temperature and pH optima. The temperature-activity profile of PPO S. melongenas and PPO M. sapientum showed a strong positive correlation ( r = 0.907363). At pH = 10.0, PPO M. sapientum activity represented 65.3% decay in enzyme activity, whereas PPO S. melongenas represented 79.3% decay in enzyme activity. PPOS.melongenas and PPO M. sapientum stability at pre-incubated temperatures of 20, 50 and 60°C and pH values of 3.5, 6.0 and 8.0 were measured. Residual activities of PPO S.melongenas and PPO M. sapientum showed a strong positive correlations under the same experimental thermal conditions, with exception at 20°C ( r = 0.693375). Specifically, pre-incubation of PPO M.sapientum for t = 90 min at 60°C caused 18.4% decay in relative activity of PPO M. sapientum . At t = 90 min, pre-incubation of PPO M. sapientum , at pH = 3.5 caused decay in activity within the range of 30.8-36.1%, whereas PPO M. sapientum pre-incubated in pH = 6.0 and pH = 8.0 gave decay in activity within the range of (1.5-9.8%) and (2.7-6.5%) respectively. PPO S.melongenas and PPO M. sapientum showed relatively higher stabilities as the incubation pH tended towards alkaline conditions, whereas the two experimental temperatures (20 and 60°C) promoted destabilization. Keywords : Polyphenol oxidase, temperature, pH, Solanum melongenas and Musa sapientum . African Journal of Biotechnology Vol. 12(38), pp. 5688-5694","PeriodicalId":142158,"journal":{"name":"American Journal of Plant Physiology","volume":"47 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122181458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2012-06-19DOI: 10.3923/AJPP.2012.135.142
L. D. S. Borges, M. Vieira, M. Marques, F. Vianello, G. Lima
Department of Chemistry and Biochemistry Institute of Biosciences Universidade Estadual Paulista, UNESP, CP 510, CEP 18.618-000, Botucatu, Sao Paulo
圣保罗国立大学生物科学研究所，cp510, CEP 18618000，圣保罗博图卡图
{"title":"Influence of organic and mineral soil fertilization on essential oil of Spilanthes oleracea cv. Jambuarana","authors":"L. D. S. Borges, M. Vieira, M. Marques, F. Vianello, G. Lima","doi":"10.3923/AJPP.2012.135.142","DOIUrl":"https://doi.org/10.3923/AJPP.2012.135.142","url":null,"abstract":"Department of Chemistry and Biochemistry Institute of Biosciences Universidade Estadual Paulista, UNESP, CP 510, CEP 18.618-000, Botucatu, Sao Paulo","PeriodicalId":142158,"journal":{"name":"American Journal of Plant Physiology","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2012-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121201678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2012-06-01DOI: 10.3923/AJPP.2012.252.260
S. Islam, E. Izekor, J. O. Garner
{"title":"Lipid and fatty acid compositions of chilling tolerant sweet potato (Ipomoea batatas L.) genotypes","authors":"S. Islam, E. Izekor, J. O. Garner","doi":"10.3923/AJPP.2012.252.260","DOIUrl":"https://doi.org/10.3923/AJPP.2012.252.260","url":null,"abstract":"","PeriodicalId":142158,"journal":{"name":"American Journal of Plant Physiology","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2012-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128579852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2012-06-01DOI: 10.3923/AJPP.2012.261.268
O. S. Hussein
{"title":"Protein Electrophoresis and DNA in Herbs Produced from Irradiated Ambrosia maritima Seeds Grown under Soil Salinity and Their Resistance to Insect","authors":"O. S. Hussein","doi":"10.3923/AJPP.2012.261.268","DOIUrl":"https://doi.org/10.3923/AJPP.2012.261.268","url":null,"abstract":"","PeriodicalId":142158,"journal":{"name":"American Journal of Plant Physiology","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2012-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122328564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2012-06-01DOI: 10.3923/AJPP.2012.243.251
G. Nkansah, J. Ofosu-Anim, A. Mawuli
{"title":"Gibberellic acid and naphthalene acetic acid affect fruit retention, yield and quality of keitt mangoes in the coastal Savanna ecological zone of Ghana","authors":"G. Nkansah, J. Ofosu-Anim, A. Mawuli","doi":"10.3923/AJPP.2012.243.251","DOIUrl":"https://doi.org/10.3923/AJPP.2012.243.251","url":null,"abstract":"","PeriodicalId":142158,"journal":{"name":"American Journal of Plant Physiology","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2012-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133901568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2012-06-01DOI: 10.3923/AJPP.2012.220.231
A. Husen, S. Khatoon
{"title":"Role of Anthraquinones as a Marker of Juvenility and Maturity in Response to Adventitious Rooting of Tectona grandis","authors":"A. Husen, S. Khatoon","doi":"10.3923/AJPP.2012.220.231","DOIUrl":"https://doi.org/10.3923/AJPP.2012.220.231","url":null,"abstract":"","PeriodicalId":142158,"journal":{"name":"American Journal of Plant Physiology","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2012-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128366401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2012-06-01DOI: 10.3923/AJPP.2012.232.242
Monsuang Rueangkhan, R. Chiarawipa, Yi Wang, X. Z. Zhang, Xuefeng Xu, Zhenhai Han
{"title":"Soil Water Supply during Dry Weather Constraint of Sweet Orange (Citrus sinensis L. Osb.) Seedlings in Response to Gas Exchanges, Photosynthetic Functions, Leaf Carbohydrate Contents and Biomass Production","authors":"Monsuang Rueangkhan, R. Chiarawipa, Yi Wang, X. Z. Zhang, Xuefeng Xu, Zhenhai Han","doi":"10.3923/AJPP.2012.232.242","DOIUrl":"https://doi.org/10.3923/AJPP.2012.232.242","url":null,"abstract":"","PeriodicalId":142158,"journal":{"name":"American Journal of Plant Physiology","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2012-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129793354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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