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Learning Curve of Resident Surgeons for Open Mesh Repair of Inguinal Hernia 腹股沟疝开放式疝修补术住院医师的学习曲线
IF 0.1 4区 医学 Q4 SURGERY Pub Date : 2022-03-01 DOI: 10.9738/intsurg-d-20-00002.1
J. Ueda, H. Yoshida
We evaluated the effect of postgraduate surgical education on inguinal hernia repair for resident surgeons. A total of 93 adult patients underwent open mesh repairs for inguinal hernias. These patients were randomly assigned to junior or senior resident surgeon groups for inguinal hernia repairs. The surgical training program for inguinal hernia repair was subdivided into 3 consecutive steps: sections A, B, and C. The sections were defined as follows: (A) the starting point of the surgery to hanging the spermatic cord, (B) dissection of the hernia sac, and (C) placement of the hernia mesh. The time to complete each procedure was recorded. We then evaluated the learning curve of the junior resident surgeons for open mesh repair of inguinal hernia. The mean operative time of the junior resident group was significantly longer than that of the senior resident group. Particularly, the mean times of the junior residents for sections B and C were significantly longer than those of the senior resident group. However, the volume of intraoperative blood loss and the short-term outcomes were not significantly different between the groups. For section C of the procedure, the learning curve seemed shorter for junior resident surgeons who had completed the training program for inguinal hernia repair than that for junior resident surgeons who were just beginning this program. This study demonstrates that a junior resident surgeon's initial experience with hernia repair is associated with an identifiable learning curve when participating in a suitable training program.
我们评估了研究生外科教育对住院外科医生腹股沟疝修补术的影响。共有93名成年患者接受了腹股沟疝的开放式网片修补术。这些患者被随机分配到初级或高级住院外科医生组进行腹股沟疝修补术。腹股沟疝修补术的手术训练计划分为3个连续步骤:A、B和C部分。这些部分的定义如下:(A)悬吊精索的手术起点,(B)疝囊的解剖,以及(C)疝网的放置。记录完成每个程序的时间。然后,我们评估了初级住院外科医生在腹股沟疝开孔疝修补术中的学习曲线。初级住院组的平均手术时间明显长于高级住院组。特别是,B和C区的初级居民的平均时间明显长于高级居民组。然而,两组之间的术中失血量和短期结果没有显著差异。对于手术的C部分,完成腹股沟疝修补培训项目的初级住院外科医生的学习曲线似乎比刚刚开始该项目的初级常驻外科医生更短。这项研究表明,初级住院外科医生在参加适当的培训计划时,其疝修补术的初始经验与可识别的学习曲线有关。
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引用次数: 0
Patient-Controlled Intravenous Analgesia With Tramadol and Lornoxicam After Thoracotomy: A Comparison With Patient-Controlled Epidural Analgesia 患者控制的曲马多和氯诺昔康开胸术后静脉镇痛与患者控制的硬膜外镇痛的比较
IF 0.1 4区 医学 Q4 SURGERY Pub Date : 2022-03-01 DOI: 10.9738/intsurg-d-16-00252.1
Ju-ying Jin, S. Min, Qibin Chen, Dong Zhang
To determine efficacy and safety of patient-controlled intravenous analgesia (PCIA) with tramadol and lornoxicam for postoperative analgesia, and its effects on surgical outcomes in patients after thoracotomy. Adequate pain relief after thoracic surgery is of particular importance, not only for keeping patients comfortable but also for reducing the incidence of postoperative complications. PCIA with tramadol and lornoxicam could be an acceptable alternative to patient-controlled epidural analgesia (PCEA) for pain management after thoracotomy. The records of patients who underwent thoracotomy for lung resection between January 2014 and December 2014 at our institution were reviewed. The patients were divided into 2 groups according to postoperative pain treatment modalities. Patients of PCEA group (n = 63) received PCEA with 0.2% ropivacaine plus 0.5 μg/mL sufentanil, while patients in PCIA group (n = 48) received PCIA with 5 mg/mL tramadol and 0.4 mg/mL lornoxicam. Data were collected for quality of pain control, incidences of analgesia-related side effects and pulmonary complications, lengths of thoracic intensive care unit stay and postoperative hospital stay, and in-hospital mortality. Pain at rest was controlled well in both groups during a 4-day postoperative period. Patients in PCIA group reported significantly higher pain scores on coughing and during mobilization in the first 2 postoperative days. The incidences of side effects and pulmonary complications, in-hospital mortality, and other outcomes were similar between groups. PCIA with tramadol and lornoxicam can be considered as a safe and effective alternative with respect to pain control and postoperative outcomes after thoracotomy.
探讨曲马多联合氯诺昔康患者自控静脉镇痛(PCIA)用于开胸术后镇痛的有效性、安全性及其对手术结局的影响。胸外科手术后适当的疼痛缓解尤为重要,不仅可以使患者保持舒适,还可以减少术后并发症的发生。曲马多加氯诺西康的硬膜外镇痛是开胸术后患者自控硬膜外镇痛(PCEA)的一种可接受的替代方法。回顾我院2014年1月至2014年12月行开胸肺切除术患者的记录。根据术后疼痛治疗方式将患者分为两组。PCEA组(63例)采用0.2%罗哌卡因+ 0.5 μg/mL舒芬太尼的PCEA治疗,PCIA组(48例)采用曲马多5 mg/mL +氯诺昔康0.4 mg/mL的PCEA治疗。收集疼痛控制质量、镇痛相关副作用和肺部并发症的发生率、胸部重症监护病房住院时间和术后住院时间以及住院死亡率的数据。术后4天,两组静息疼痛控制良好。PCIA组患者术后2天咳嗽和活动时疼痛评分明显增高。副作用和肺部并发症的发生率、住院死亡率和其他结果在两组之间相似。对于开胸术后疼痛控制和术后结果而言,曲马多和氯诺昔康联合PCIA可以被认为是一种安全有效的选择。
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引用次数: 1
Editorial Letter for International Surgery Issue 106 (2Q, 2022) 《国际外科杂志》第106期(2022年第二季)社论
IF 0.1 4区 医学 Q4 SURGERY Pub Date : 2022-03-01 DOI: 10.9738/0020-8868-106.2.i
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引用次数: 0
Preoperative Oral-Bowel Preparation Using Sodium Picosulfate and Magnesium Citrate Combination Powder for Left-Sided Colorectal Cancer: A Prospective Study 应用Pico硫酸钠和柠檬酸镁复合粉在左大肠癌术前口服制剂的前瞻性研究
IF 0.1 4区 医学 Q4 SURGERY Pub Date : 2022-03-01 DOI: 10.9738/intsurg-d-20-00038.1
K. Ohta, M. Ikenaga, M. Ueda, Kiyotsugu Iede, Yujiro Tsuda, S. Nakashima, Tsukasa Tanida, T. Nojiri, J. Matsuyama, S. Endo, Terumasa Yamada
This study aimed to determine if induction of sodium picosulfate and magnesium citrate (SPMC) preparation leads to adequate surgery for left-sided colorectal cancer (CRC). Adequate bowel cleansing is vital for safe and accurate colorectal surgery. Ingestion of laxatives for bowel cleansing may be associated with a broad spectrum of adverse effects during surgical intervention. A single-center prospective study was conducted with hospitalized patients scheduled operation for left-sided CRC. All enrolled patients were instructed to consume normal diet until before preparation. Twenty-seven patients underwent preoperative SPMC preparation. The primary endpoint of this study was the 30-day postoperative morbidity rate, which was 23%, and no postoperative complication was higher than Clavien-Dindo grade 3. The primary anastomosis rate was 100%, and there was no anastomotic leakage. The colonic cleansing grades were 1 or 2 according to the bowel preparation scale for surgical assessment in 89% of the patients. The acceptability of the cleansing procedure by the patients assessed by a questionnaire was 85%, and the acceptability of the SPMC preparation by the medical staff was 93%. The surgical procedures included 18 laparoscopies, 6 robotic surgeries, and 3 laparotomies. The median operation time was 165 minutes, and the median blood loss was <50 cc. The median sodium serum concentration was significantly decreased after surgery. Ingestion of an SPMC preparation as a cleansing procedure was judged to be adequate for curative surgery in patients with left-sided colorectal cancer.
本研究旨在确定皮硫酸钠和柠檬酸镁(SPMC)制备诱导是否导致左侧结直肠癌(CRC)的适当手术。充分的肠道清洁对于安全和准确的结直肠手术至关重要。在手术干预期间,肠道清洁的泻药摄入可能与广泛的不良反应有关。一项单中心前瞻性研究对住院患者进行左侧结直肠癌手术。所有入组的患者在准备前都要正常饮食。27例患者行术前SPMC准备。本研究的主要终点是术后30天的发病率,为23%,无术后并发症高于Clavien-Dindo 3级。一期吻合率100%,无吻合口漏。89%的患者结肠清洁等级为1级或2级,根据肠准备量表进行手术评估。通过问卷评估,患者对清洁程序的可接受度为85%,医务人员对SPMC制剂的可接受度为93%。手术包括18例腹腔镜手术、6例机器人手术和3例剖腹手术。中位手术时间165分钟,中位失血量<50 cc,术后中位血钠浓度明显降低。摄取SPMC制剂作为清洁程序被认为是足够的治疗手术患者的左侧结直肠癌。
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引用次数: 0
Effects of Fasting and Administration of Octreotide Acetate and Ulinastatin on Clinical Outcomes of Pancreatic Fistula after Pancreatoduodenectomy 禁食及给予醋酸奥曲肽和乌司他丁对胰十二指肠切除术后胰瘘临床疗效的影响
IF 0.1 4区 医学 Q4 SURGERY Pub Date : 2022-02-18 DOI: 10.9738/intsurg-d-21-00022.1
Kyohei Abe, Y. Futagawa, H. Shiba, K. Furukawa, S. Onda, Masaru Kanehira, T. Sakamoto, T. Uwagawa, Y. Ishida, K. Yanaga
Objective: Postoperative pancreatic fistula (POPF) following pancreaticoduodenectomy is the most serious complication of these surgical procedures; therefore, we examine the effectiveness of fasting, and administration of octreotide acetate and ulinastatin as a method of prevention. Summary of Background Data : Although various drug therapies and surgical techniques have been used for the treatment of POPF, no decisive treatment for POPF exists. Methods: The clinical course of 30 patients who developed POPF was retrospectively evaluated and compared between no dietary intake (n=18), octreotide acetate (n=8), and ulinastatin (n=8) using an overlapping design. Patients were allocated to either the dietary intake or fasting (no dietary intake) group, and those in the no dietary intake group were further divided into the octreotide acetate or ulinastatin group. Results: Length of hospitalization was longer for the no dietary intake group than for the dietary intake group (p=0.002). When considering only grade B or C POPF cases, the no dietary intake group had a longer length of hospitalization and a higher white blood cell count on day 7 after the diagnosis of POPF than the dietary intake group (p<0.05). The white blood cell count was also higher in the octreotide acetate group than in the ulinastatin group (p=0.021). The length of hospitalization was shorter in the ulinastatin group than in the octreotide acetate group (p=0.025). Conclusions: The use of no dietary intake, octreotide acetate, and ulinastatin do not seem to contribute to the clinical course of patients with POPF after pancreatoduodenectomy.
目的:胰十二指肠切除术后胰瘘(POPF)是这些手术中最严重的并发症;因此,我们检查禁食的有效性,并将醋酸奥曲肽和乌司他丁作为一种预防方法。背景数据摘要:尽管已经使用了各种药物疗法和手术技术来治疗POPF,但还没有对POPF的决定性治疗方法。方法:采用重叠设计,对30例发生POPF的患者的临床病程进行回顾性评估,并在无饮食摄入(n=18)、醋酸奥曲肽(n=8)和乌司他丁(n=8。患者被分为饮食摄入组或禁食(无饮食摄入)组,无饮食摄入组的患者被进一步分为醋酸奥曲肽组或乌司他丁组。结果:无饮食摄入组的住院时间长于饮食摄入组(p=0.002)。当仅考虑B或C级POPF病例时,与饮食摄入组相比,无饮食摄入组在诊断POPF后第7天的住院时间更长,白细胞计数更高(p<0.05)。醋酸奥曲肽组的白细胞计数也高于乌司他丁组(p=0.021)结论:不进食、醋酸奥曲肽和乌司他丁似乎对胰十二指肠切除术后POPF患者的临床病程没有影响。
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引用次数: 0
Raymond A Dieter, Jr. MD MS 雷蒙德·A·迪特,医学博士
IF 0.1 4区 医学 Q4 SURGERY Pub Date : 2022-01-01 DOI: 10.9738/0020-8868-106.1.i
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引用次数: 0
Feasibility of Percutaneous Dilatational Tracheotomy in Head and Neck Cancer Surgery: A Preliminary Study 经皮扩张性气管切开术在头颈部肿瘤手术中的可行性:初步研究
IF 0.1 4区 医学 Q4 SURGERY Pub Date : 2022-01-01 DOI: 10.9738/intsurg-d-21-00015.1
H. Lee, Y. Park, Rip Kim, S. Kang, Inn-Chul Nam
Percutaneous dilational tracheostomy (PDT) is a technique that can place a tracheostomy tube safely without visually identifying the trachea. We evaluated its feasibility during head and neck cancer surgery. PDT has many advantages, such as less bleeding, easier technique, and shorter procedural time. Twelve patients who underwent PDT during head and neck cancer surgery from September 2016 to March 2018 were enrolled, and their medical records were reviewed retrospectively. Medical records of another 12 patients who underwent conventional tracheostomy during head and neck cancer surgery were analyzed. PDT was performed using a Ciaglia Percutaneous Tracheostomy Set. The tracheostomy point was determined by palpation without the guidance of bronchoscopy or ultrasonography. Blood loss, procedural time, communication between the cervical wound and tracheostomy wound, and complications were compared between the PDT group and the conventional group. The PDT group had less blood loss, a shorter procedural time, and a lower incidence of communication between the cervical and tracheostomy wound. There was 1 case of conversion to conventional tracheostomy due to wrong tracheal penetration in the PDT group. PDT is safe and effective as an adjunctive procedure during head and neck cancer surgery.
经皮扩张性气管造口术(PDT)是一种无需肉眼识别气管即可安全地放置气管造口管的技术。我们评估了其在头颈部肿瘤手术中的可行性。PDT具有出血少、技术简单、手术时间短等优点。本研究纳入了2016年9月至2018年3月期间在头颈癌手术中接受PDT治疗的12例患者,并对其医疗记录进行回顾性分析。分析了另外12例在头颈癌手术中接受常规气管切开术的患者的医疗记录。PDT采用Ciaglia经皮气管切开术。在没有支气管镜或超声的指导下,通过触诊确定气管切开术点。比较PDT组与常规组的出血量、手术时间、颈部创口与气管造口创口之间的连通性及并发症。PDT组出血量少,手术时间短,颈部创口与气管造口创口相通的发生率低。PDT组有1例因气管穿刺错误而转为常规气管切开术。PDT作为头颈癌手术的辅助手术是安全有效的。
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引用次数: 1
Laparoscopic Right Hemicolectomy of a Low-Grade Appendiceal Mucinous Neoplasm Causing an Ileocolic Intussusception: A Case Report 腹腔镜右半结肠切除术治疗低位阑尾粘液性肿瘤致肠绞痛肠套叠1例
IF 0.1 4区 医学 Q4 SURGERY Pub Date : 2022-01-01 DOI: 10.9738/intsurg-d-20-00039.1
Gang-Hua Lin, Ta-Wei Pu, Yu-Chun Lin, C. Chang, Yu-Jen Chen, Jung-Cheng Kang, C. Hsiao, Chao-Yang Chen, Je-Ming Hu
Intussusception, although common in the pediatric population, rarely occurs in adults. Furthermore, patients often show nonspecific symptoms. Most adult patients with intussusception have a surgical lead point, a well-defined pathological abnormality, often accurately diagnosed after surgery. A low-grade appendiceal mucinous neoplasm (LAMN), often misdiagnosed as acute appendicitis, is rarely associated with the development of intussusception. Here we report a case of LAMN-related ileocolic intussusception that was histologically diagnosed after laparoscopic right hemicolectomy. A 58-year-old woman visited our emergency department because of intermittent episodes of epigastric pain with periumbilical tenderness. These symptoms persisted intermittently for 2 weeks. The pain was moderate in severity, colicky in nature, and sometimes shifted to the lower abdominal region. Abdominal computed tomography indicated intussusception with ileocecal and mesenteric telescoping into the transverse colon. Complete colonoscopy with reduction of intussusception was performed, revealing a ball-like mass protruding and occupying the entire cecum lumen. Laparoscopic right hemicolectomy was then performed. Macroscopically, a dilated appendix was revealed with mucin content, along with hyalinization and fibrosis of the appendiceal wall. Microscopically, a tumor exhibiting villous and flat proliferation of mucinous epithelial cells with low-grade nuclear atypia was seen. However, there was no infiltration growth of the tumor cells, thereby demonstrating LAMN. Postoperative recovery was uneventful, and the patient was discharged on the 8th postoperative day without surgical complications. The differential diagnoses of chronic and colicky abdominal pain should be expanded to include intussusceptions as they can be severe, although cases in adults are rare.
肠套叠虽然在儿科人群中很常见,但在成人中很少发生。此外,患者经常表现出非特异性症状。大多数成年肠套叠患者都有手术引导点,这是一种明确的病理异常,通常在手术后准确诊断。低级别阑尾粘液性肿瘤(LAMN),常被误诊为急性阑尾炎,很少与肠套叠的发展有关。在此,我们报告了一例LAMN相关的回结肠肠套叠,该病例在腹腔镜右半结肠切除术后经组织学诊断。一位58岁的女性因间歇性上腹部疼痛伴脐周压痛而就诊于我们的急诊科。这些症状间歇性持续2周。疼痛程度中等,性质为绞痛,有时会转移到下腹部。腹部计算机断层扫描显示肠套叠,回盲部和肠系膜伸缩到横结肠中。进行了肠套叠复位的完整结肠镜检查,发现一个球状肿块突出并占据了整个盲肠腔。然后进行腹腔镜右半结肠切除术。肉眼可见阑尾扩张,伴有粘蛋白含量,阑尾壁透明化和纤维化。显微镜下,肿瘤表现出绒毛状和扁平的粘液上皮细胞增殖,伴有低度核异型性。然而,肿瘤细胞没有浸润生长,从而显示LAMN。术后恢复顺利,患者于术后第8天出院,无手术并发症。慢性和绞痛性腹痛的鉴别诊断应扩大到包括肠套叠,因为它们可能很严重,尽管成人病例很少。
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引用次数: 0
Boari flap urinary tract reconstruction for rectosigmoid cancer with ureteral invasion: Report of a case 膀胱瓣尿道重建术治疗乙状结肠直肠癌伴输尿管侵犯1例
IF 0.1 4区 医学 Q4 SURGERY Pub Date : 2021-11-29 DOI: 10.9738/intsurg-d-21-00017.1
Kenji Koneri, T. Goi, M. Shimada, Noriyuki Tagai, Hidetaka Kurebayashi, K. Sawai, M. Morikawa, M. Tamaki, M. Murakami, Y. Hirono, Y. Aoki, O. Yokoyama
Introduction: The Boari flap technique is a unique urinary tract reconstruction procedure performed after resection of the urinary tract. However, few previous reports have described the application of this technique to gastrointestinal cancer. Moreover, we have not found any papers describing the long-term prognosis. We report a case of right ureteral tract resection followed by Boari flap reconstruction for rectosigmoid carcinoma, with survival for 108 months without any urological complications. Case presentation: A woman in her 50s was diagnosed with rectosigmoid caner by local physician and referred to our institution. Computed tomography revealed right hydronephrosis due to rectosigmoid cancer invasion at the lower two-thirds of the right ureter. During laparotomy, massive lymphatic infiltration from the primary lesion to right ureter was observed. After primary tumor resection with lower ureter excision, the Boari flap procedure was performed to reconstruct the ureteral deficit. Postoperative course was uneventful, and she was discharged on postoperative day 20. The patient has been followed every 4 months for 9 years with no recurrence or unpleasant symptoms. Discussion: This technique is usually performed to manage specific conditions such as ureteral stenosis caused by ureteral calculi, retroperitoneal fibrosis, and gynecological disorders. This procedure should be reconsidered as a possible option for gastrointestinal malignant cases instead of nephrostomy or cutaneous ureterostomy, given the low rate of complications and high patient satisfaction. Conclusion: The Boari flap technique is particularly useful for bridging between the ureter and bladder in cases of colorectal malignancy with combined resection of the lower urinary tract.
简介:Boari皮瓣技术是一种在泌尿道切除后进行的独特的泌尿道重建手术。然而,很少有先前的报道描述了该技术在胃肠道癌症中的应用。此外,我们尚未发现任何描述长期预后的论文。我们报告一例右输尿管切除术后行直肠乙状结肠癌Boari皮瓣重建术,存活108个月,无任何泌尿系统并发症。病例介绍:一名50多岁的妇女被当地医生诊断为直肠乙状结肠癌并转介到我们的机构。计算机断层扫描显示右侧肾积水是由于直肠乙状结肠癌侵袭右输尿管的下三分之二。开腹时,发现大量淋巴从原发病灶向右输尿管浸润。在原发肿瘤切除及输尿管下段切除后,采用Boari皮瓣重建输尿管缺损。术后过程顺利,于术后第20天出院。患者每4个月随访9年,无复发或不良症状。讨论:该技术通常用于治疗特殊情况,如输尿管结石引起的输尿管狭窄、腹膜后纤维化和妇科疾病。考虑到并发症发生率低,患者满意度高,对于胃肠道恶性病例,应重新考虑该手术作为替代肾造口术或皮肤输尿管造口术的可能选择。结论:在结直肠恶性肿瘤联合下尿路切除的病例中,Boari皮瓣技术在输尿管与膀胱之间的桥接尤其有效。
{"title":"Boari flap urinary tract reconstruction for rectosigmoid cancer with ureteral invasion: Report of a case","authors":"Kenji Koneri, T. Goi, M. Shimada, Noriyuki Tagai, Hidetaka Kurebayashi, K. Sawai, M. Morikawa, M. Tamaki, M. Murakami, Y. Hirono, Y. Aoki, O. Yokoyama","doi":"10.9738/intsurg-d-21-00017.1","DOIUrl":"https://doi.org/10.9738/intsurg-d-21-00017.1","url":null,"abstract":"Introduction: The Boari flap technique is a unique urinary tract reconstruction procedure performed after resection of the urinary tract. However, few previous reports have described the application of this technique to gastrointestinal cancer. Moreover, we have not found any papers describing the long-term prognosis. We report a case of right ureteral tract resection followed by Boari flap reconstruction for rectosigmoid carcinoma, with survival for 108 months without any urological complications. Case presentation: A woman in her 50s was diagnosed with rectosigmoid caner by local physician and referred to our institution. Computed tomography revealed right hydronephrosis due to rectosigmoid cancer invasion at the lower two-thirds of the right ureter. During laparotomy, massive lymphatic infiltration from the primary lesion to right ureter was observed. After primary tumor resection with lower ureter excision, the Boari flap procedure was performed to reconstruct the ureteral deficit. Postoperative course was uneventful, and she was discharged on postoperative day 20. The patient has been followed every 4 months for 9 years with no recurrence or unpleasant symptoms. Discussion: This technique is usually performed to manage specific conditions such as ureteral stenosis caused by ureteral calculi, retroperitoneal fibrosis, and gynecological disorders. This procedure should be reconsidered as a possible option for gastrointestinal malignant cases instead of nephrostomy or cutaneous ureterostomy, given the low rate of complications and high patient satisfaction. Conclusion: The Boari flap technique is particularly useful for bridging between the ureter and bladder in cases of colorectal malignancy with combined resection of the lower urinary tract.","PeriodicalId":14474,"journal":{"name":"International surgery","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2021-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48326270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Application of ica D, eno, sar A and agr gene testing in early diagnosis of periprosthetic joint infection ica D、eno、sar A、agr基因检测在假体周围关节感染早期诊断中的应用
IF 0.1 4区 医学 Q4 SURGERY Pub Date : 2021-11-03 DOI: 10.9738/intsurg-d-21-00021.1
Jie Lin, Yong-Xie Jin, Q. Pang
Objective This study intends to explore the diagnostic efficiency and value of PCR in the early diagnosis of periprosthetic joint infection (PJI) based on the testing of the four staphylococcal specific genes ica D, eno, sar A and agr. Methods 41 samples of ultrasonic cleavage RNA eluate were extracted from the human joint model of PJI caused by the five most common PJI pathogens to detect the PCR of ica D, eno, sar A and agr genes. Based on the detection results, the sensitivity, specificity, positive and negative predictive value and accuracy of the four genes were analyzed and compared. Results eno test had a high sensitivity (83.33%). However, more false positive results affected the test specificity (47.06%); sar A test did not show false positive results, but the specificity was high (100%), but the sensitivity was low (41.67%); the sensitivity of ica D and agr test was 4.17% and 0%, respectively, and the clinical value was limited. Conclusion eno and sar A gene detection is of high value in early diagnosis of PJI. Reasonable design and sequential application of eno sensitivity preliminary diagnosis and sar A specific screening of false positive results are more beneficial to the early diagnosis of staphylococcal pathogenic PJI.
目的通过检测4个葡萄球菌特异性基因ica D、eno、sar A和agr,探讨聚合酶链式反应在假体周围关节感染早期诊断中的有效性和价值。方法从5种最常见的PJI病原体引起的PJI人类关节模型中提取41份超声切割RNA洗脱液样品,对ica D、eno、sar A和agr基因进行PCR检测。根据检测结果,对四个基因的敏感性、特异性、阳性和阴性预测值及准确性进行了分析和比较。结果eno检测灵敏度高(83.33%),但假阳性率越高,特异性越差(47.06%);sar A检测未出现假阳性结果,但特异性高(100%),敏感性低(41.67%);ica D和agr试验的敏感性分别为4.17%和0%,临床价值有限。结论eno和sar A基因检测对PJI的早期诊断具有较高价值。合理设计和顺序应用eno敏感性初步诊断和sar特异性筛查假阳性结果更有利于葡萄球菌致病性PJI的早期诊断。
{"title":"Application of ica D, eno, sar A and agr gene testing in early diagnosis of periprosthetic joint infection","authors":"Jie Lin, Yong-Xie Jin, Q. Pang","doi":"10.9738/intsurg-d-21-00021.1","DOIUrl":"https://doi.org/10.9738/intsurg-d-21-00021.1","url":null,"abstract":"Objective This study intends to explore the diagnostic efficiency and value of PCR in the early diagnosis of periprosthetic joint infection (PJI) based on the testing of the four staphylococcal specific genes ica D, eno, sar A and agr. Methods 41 samples of ultrasonic cleavage RNA eluate were extracted from the human joint model of PJI caused by the five most common PJI pathogens to detect the PCR of ica D, eno, sar A and agr genes. Based on the detection results, the sensitivity, specificity, positive and negative predictive value and accuracy of the four genes were analyzed and compared. Results eno test had a high sensitivity (83.33%). However, more false positive results affected the test specificity (47.06%); sar A test did not show false positive results, but the specificity was high (100%), but the sensitivity was low (41.67%); the sensitivity of ica D and agr test was 4.17% and 0%, respectively, and the clinical value was limited. Conclusion eno and sar A gene detection is of high value in early diagnosis of PJI. Reasonable design and sequential application of eno sensitivity preliminary diagnosis and sar A specific screening of false positive results are more beneficial to the early diagnosis of staphylococcal pathogenic PJI.","PeriodicalId":14474,"journal":{"name":"International surgery","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2021-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42419947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
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International surgery
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