Pub Date : 2022-05-01DOI: 10.1164/ajrccm-conference.2022.205.1_meetingabstracts.a1156
E. Boers, M. Barrett, V. Vuong, J. Su, L. Kaye, D. Tellez, A. Benjafield, C. Nunez, A. Malhotra
{"title":"Global Projections Regarding Smoking Prevalence Trends: A Model Based Analysis","authors":"E. Boers, M. Barrett, V. Vuong, J. Su, L. Kaye, D. Tellez, A. Benjafield, C. Nunez, A. Malhotra","doi":"10.1164/ajrccm-conference.2022.205.1_meetingabstracts.a1156","DOIUrl":"https://doi.org/10.1164/ajrccm-conference.2022.205.1_meetingabstracts.a1156","url":null,"abstract":"","PeriodicalId":148653,"journal":{"name":"A25. TOBACCO AND THC HEALTH SCIENCE","volume":"78 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116759388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-01DOI: 10.1164/ajrccm-conference.2022.205.1_meetingabstracts.a1150
R. Pinkston, A. Penn, A. Noel
{"title":"Exposure to JUUL Menthol-Flavored Aerosol Modulates House-Dust Mite-Induced Asthma in Wild-Type Juvenile Female Mice Comparably to Mice with a MMP12 Deficiency","authors":"R. Pinkston, A. Penn, A. Noel","doi":"10.1164/ajrccm-conference.2022.205.1_meetingabstracts.a1150","DOIUrl":"https://doi.org/10.1164/ajrccm-conference.2022.205.1_meetingabstracts.a1150","url":null,"abstract":"","PeriodicalId":148653,"journal":{"name":"A25. TOBACCO AND THC HEALTH SCIENCE","volume":"38 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128805732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-01DOI: 10.1164/ajrccm-conference.2022.205.1_meetingabstracts.a1146
J. Marganski, I. Advani, S. Boddu, S. Patel, D. Gunge, W. Merz, A.L. Fuentes Baldarrago, A. Malhotra, L. C. Crotty Alexander
{"title":"A Study Assessing Interactions of E-cigarette and Other Inhalants with Sleep Quality in Subjects Recruited Between 2018 and 2020","authors":"J. Marganski, I. Advani, S. Boddu, S. Patel, D. Gunge, W. Merz, A.L. Fuentes Baldarrago, A. Malhotra, L. C. Crotty Alexander","doi":"10.1164/ajrccm-conference.2022.205.1_meetingabstracts.a1146","DOIUrl":"https://doi.org/10.1164/ajrccm-conference.2022.205.1_meetingabstracts.a1146","url":null,"abstract":"","PeriodicalId":148653,"journal":{"name":"A25. TOBACCO AND THC HEALTH SCIENCE","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123902734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-01DOI: 10.1164/ajrccm-conference.2022.205.1_meetingabstracts.a1145
M.-S. Lee, J. Allen, K. Eum, J. Onnela, D. Christiani
{"title":"Vaping Habits and Respiratory Health Outcomes Using a Smartphone App Platform","authors":"M.-S. Lee, J. Allen, K. Eum, J. Onnela, D. Christiani","doi":"10.1164/ajrccm-conference.2022.205.1_meetingabstracts.a1145","DOIUrl":"https://doi.org/10.1164/ajrccm-conference.2022.205.1_meetingabstracts.a1145","url":null,"abstract":"","PeriodicalId":148653,"journal":{"name":"A25. TOBACCO AND THC HEALTH SCIENCE","volume":"104 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122119832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-01DOI: 10.1164/ajrccm-conference.2022.205.1_meetingabstracts.a1148
J. Meehan-Atrash, I. Rahman
{"title":"Novel Delta-8-Tetrahydrocannabinol Vaporizers Contain Unlabeled Adulterants, Byproducts of Chemical Synthesis, and Heavy Metals","authors":"J. Meehan-Atrash, I. Rahman","doi":"10.1164/ajrccm-conference.2022.205.1_meetingabstracts.a1148","DOIUrl":"https://doi.org/10.1164/ajrccm-conference.2022.205.1_meetingabstracts.a1148","url":null,"abstract":"","PeriodicalId":148653,"journal":{"name":"A25. TOBACCO AND THC HEALTH SCIENCE","volume":"92 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131433634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-01DOI: 10.1164/ajrccm-conference.2022.205.1_meetingabstracts.a1149
N. Durmus, G. Grunig, A. Raja, V. Goriainova, H. Joung, D. Chalupa, A. Elder, J. Zelikoff
{"title":"Long-Term Toxicity of E-Cigarette Whole Body Aerosol Exposure Using Cardiovascular Health and Pulmonary Changes in Mice as Persistent Outcomes","authors":"N. Durmus, G. Grunig, A. Raja, V. Goriainova, H. Joung, D. Chalupa, A. Elder, J. Zelikoff","doi":"10.1164/ajrccm-conference.2022.205.1_meetingabstracts.a1149","DOIUrl":"https://doi.org/10.1164/ajrccm-conference.2022.205.1_meetingabstracts.a1149","url":null,"abstract":"","PeriodicalId":148653,"journal":{"name":"A25. TOBACCO AND THC HEALTH SCIENCE","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129609224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-01DOI: 10.1164/ajrccm-conference.2022.205.1_meetingabstracts.a1152
K. Batbayar, K. Ye, A. Marsh, M. Shi, S. Park, A. Dabo, P. Geraghty, B. Lucas, M. Vigodner, S. Spivack
{"title":"Investigation of Smoking Effect on Male Germline Epigenome and Its Transmission Across Generations","authors":"K. Batbayar, K. Ye, A. Marsh, M. Shi, S. Park, A. Dabo, P. Geraghty, B. Lucas, M. Vigodner, S. Spivack","doi":"10.1164/ajrccm-conference.2022.205.1_meetingabstracts.a1152","DOIUrl":"https://doi.org/10.1164/ajrccm-conference.2022.205.1_meetingabstracts.a1152","url":null,"abstract":"","PeriodicalId":148653,"journal":{"name":"A25. TOBACCO AND THC HEALTH SCIENCE","volume":"50 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115936984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-01DOI: 10.1164/ajrccm-conference.2022.205.1_meetingabstracts.a1155
N. Alexis, W. Dabbs, N. Wernsman Young, A. Hastie, C. Doerschuk, P. Woodruff, R. Paine
Introduction: Due to Covid-19 restrictions on collecting and processing sputum samples in real time in clinic, we designed a novel sputum home collection method with immediate freezing and delayed processing (“home”). A validation study was carried out to compare key sputum endpoints using the “home” vs “real time (RT)” collection and processing methods. Sputum soluble phase proteomics, mucins and RNA/DNA endpoints were measured and compared between the 2 methods to assess the validity of the “home” method. Methods: Spontaneous sputum samples were collected from N=10 healthy adult volunteers. Each sample was split evenly by weight and processed, half by the “home” method and half by the RT method. Home method samples were first aliquoted into 3 collection tubes (T) as follows: T1: 100-250mg for mucin analysis (refractive index, gel chromatography, and CsCl gradients);T2 and T3: equal weights each, T2 for proteomic analysis (MesoScale Discovery) and T3 for RNA/DNA analysis (Isohelix collection kit). Each was immediately frozen at -20 deg C (24-48hr), then at -80 deg C (2-4 weeks) without any processing. Thawed home T1 and T2 samples were processed by treating with 8M Urea (1:1) to deactivate SARS-CoV-2 if present. T1 was then stored at 2-4 deg C, and T2 was processed with 7x DPBS, centrifuged and recovered supernatants stored at -80 deg C. In contrast, the RT sputum was first treated with 8M Urea (1:1) soon after collection, and then processed for mucins and proteomics per the “home” method above. The remaining cell pellet from the RT processed sample was stored in Zymo research RNA/DNA shield (0.5ml) and, along with home T3 samples, extracted and analyzed for qualitative and quantitative yield, as well as for genes of interest. Paired T-Test analysis compared all sputum endpoints between the home and RT method. Results: There were no statistically significant differences (p<0.05) between the home and RT method for any mucin (MUC5B, MUC5AC, MUC5AC:MUC5B ratio, total mucin) or proteomic endpoint (IL-1a, IL-6, IL-8, TNFalpha, TIMP1, TIMP2, MMP-9, CRP, MPO). In addition, except for CRP and MUC5AC, correlation between sample pairings was strong (correlation coefficient R, range = 0.5-0.9) and statistically significant (p<0.05) for all sputum endpoints. RNA/DNA results are still pending. Conclusion: The sputum “home collection method with immediate freezing and delayed processing” does not result in significantly different proteomic and mucin measurements when compared to the same samples being processed in real time in an identical manner.
{"title":"A Validation Study on a Sputum Home Collection Method with Immediate Freezing and Delayed Processing: Impact on Proteomic, Mucin and RNA/DNA Endpoints","authors":"N. Alexis, W. Dabbs, N. Wernsman Young, A. Hastie, C. Doerschuk, P. Woodruff, R. Paine","doi":"10.1164/ajrccm-conference.2022.205.1_meetingabstracts.a1155","DOIUrl":"https://doi.org/10.1164/ajrccm-conference.2022.205.1_meetingabstracts.a1155","url":null,"abstract":"Introduction: Due to Covid-19 restrictions on collecting and processing sputum samples in real time in clinic, we designed a novel sputum home collection method with immediate freezing and delayed processing (“home”). A validation study was carried out to compare key sputum endpoints using the “home” vs “real time (RT)” collection and processing methods. Sputum soluble phase proteomics, mucins and RNA/DNA endpoints were measured and compared between the 2 methods to assess the validity of the “home” method. Methods: Spontaneous sputum samples were collected from N=10 healthy adult volunteers. Each sample was split evenly by weight and processed, half by the “home” method and half by the RT method. Home method samples were first aliquoted into 3 collection tubes (T) as follows: T1: 100-250mg for mucin analysis (refractive index, gel chromatography, and CsCl gradients);T2 and T3: equal weights each, T2 for proteomic analysis (MesoScale Discovery) and T3 for RNA/DNA analysis (Isohelix collection kit). Each was immediately frozen at -20 deg C (24-48hr), then at -80 deg C (2-4 weeks) without any processing. Thawed home T1 and T2 samples were processed by treating with 8M Urea (1:1) to deactivate SARS-CoV-2 if present. T1 was then stored at 2-4 deg C, and T2 was processed with 7x DPBS, centrifuged and recovered supernatants stored at -80 deg C. In contrast, the RT sputum was first treated with 8M Urea (1:1) soon after collection, and then processed for mucins and proteomics per the “home” method above. The remaining cell pellet from the RT processed sample was stored in Zymo research RNA/DNA shield (0.5ml) and, along with home T3 samples, extracted and analyzed for qualitative and quantitative yield, as well as for genes of interest. Paired T-Test analysis compared all sputum endpoints between the home and RT method. Results: There were no statistically significant differences (p<0.05) between the home and RT method for any mucin (MUC5B, MUC5AC, MUC5AC:MUC5B ratio, total mucin) or proteomic endpoint (IL-1a, IL-6, IL-8, TNFalpha, TIMP1, TIMP2, MMP-9, CRP, MPO). In addition, except for CRP and MUC5AC, correlation between sample pairings was strong (correlation coefficient R, range = 0.5-0.9) and statistically significant (p<0.05) for all sputum endpoints. RNA/DNA results are still pending. Conclusion: The sputum “home collection method with immediate freezing and delayed processing” does not result in significantly different proteomic and mucin measurements when compared to the same samples being processed in real time in an identical manner.","PeriodicalId":148653,"journal":{"name":"A25. TOBACCO AND THC HEALTH SCIENCE","volume":"53 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116772325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-01DOI: 10.1164/ajrccm-conference.2022.205.1_meetingabstracts.a1153
C. Eckhardt, P. Balte, J. Morris, S. Bhatt, D. Couper, P. Lutsey, D. Jacobs, R. Kalhan, R. Kronmal, R. Kaplan, L. Loehr, S. London, G. T. O'Connor, J. Fetterman, J. Schwartz, W. White, S. Yende, N. Freedman, T. Sanchez, E. Oelsner
{"title":"Associations of Pipe and Cigar Smoking with DNA Methylation Levels at the Aryl Hydrocarbon Receptor Repressor Gene","authors":"C. Eckhardt, P. Balte, J. Morris, S. Bhatt, D. Couper, P. Lutsey, D. Jacobs, R. Kalhan, R. Kronmal, R. Kaplan, L. Loehr, S. London, G. T. O'Connor, J. Fetterman, J. Schwartz, W. White, S. Yende, N. Freedman, T. Sanchez, E. Oelsner","doi":"10.1164/ajrccm-conference.2022.205.1_meetingabstracts.a1153","DOIUrl":"https://doi.org/10.1164/ajrccm-conference.2022.205.1_meetingabstracts.a1153","url":null,"abstract":"","PeriodicalId":148653,"journal":{"name":"A25. TOBACCO AND THC HEALTH SCIENCE","volume":"53 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117164157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-01DOI: 10.1164/ajrccm-conference.2022.205.1_meetingabstracts.a1151
D. Hinds, H. Nick, T. Vallin, C. W. White, A. Bosco-Lauth
{"title":"Effect of Vaping on Lung Inflammation and SARS-CoV-2 Infection in a Hamster Model","authors":"D. Hinds, H. Nick, T. Vallin, C. W. White, A. Bosco-Lauth","doi":"10.1164/ajrccm-conference.2022.205.1_meetingabstracts.a1151","DOIUrl":"https://doi.org/10.1164/ajrccm-conference.2022.205.1_meetingabstracts.a1151","url":null,"abstract":"","PeriodicalId":148653,"journal":{"name":"A25. TOBACCO AND THC HEALTH SCIENCE","volume":"277 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132487247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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