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Protective Effect of Edible Bird’s Nest on Ovaries of Cycling Female Rats Subjected to Cadmium Toxicity 食用燕窝对镉中毒循环雌性大鼠卵巢的保护作用
Q3 Veterinary Pub Date : 2021-01-01 DOI: 10.17582/journal.jahp/2021/9.4.496.503
A. Quddus, N. Yimer, Faez Firdaus Abdullah Jesse, M. Noordin, Mark W. H. Hiew, Muhammad Abdul Basit, Marian Amir, M. Sirajul Islam, U. Kaka
| Cadmium (Cd), a toxic heavy metal, is often associated with the reproductive disorders of mammals. The edible bird’s nest (EBN) is a naturally occurring food product made from the saliva of swiftlet birds ( Aerodramus fuci-phagus & Aerodramus maximus ) in the form of a nest, and it has been consumed as healthy food or tonic for decades. This research aimed to study the possible protective effects of EBN against Cd toxicity in ovaries of Sprague Dawley rats. Thirty (30) female Sprague Dawley rats were assigned into five groups as follows: group 1-Negative control (NC) received distilled water; group 2-positive control (PC) administered with CdCl 2 , 5mg/kg BW; while groups EBN 1, EBN 2, and EBN 3 received CdCl 2 (5mg/kg BW) concurrently with graded concentrations of 60, 90 and 120 mg/kg BW of EBN, respectively. After four weeks of treatment, rats were euthanized to collect ovaries for histopathological studies using hematoxylin and eosin (H&E) stain. Expressions of vascular endothelial growth factor (VEGF) were analyzed through immunohistochemistry, and levels of superoxidase dismutase (SOD) were assessed using a SOD assay kit. Oral administration of CdCl 2 without EBN supplement resulted in significantly decreased (p<0.05) immunohistochemical expressions of VEGF in ovarian tissue and a decrease (p<0.05) in the activity of SOD. Moreover, ovarian histopathological changes, including follicular cysts and a significant increase (p<0.05) in the number of atretic follicles, while a decrease in the number of growing follicles was noted in Cd only treated group. Animals treated with CdCl 2 and EBN at three different dosages resulted in significantly increased (p<0.05) expressions of VEGF in ovarian tissues, low degenerative changes with normal histomorphology as well as significantly increased (p<0.05) SOD activity as compared to the PC group. Overall, the findings revealed that oral exposure to Cd at a dose rate of 5 mg/kg BW resulted in significant alterations in ovaries, as evidenced by a lower degree of VEGF expression along with reduced antioxidant activity and histomorphological changes. Meanwhile, EBN proved to exhibit a significant protective role against Cd toxicity in ovaries, possibly through its antioxidant effect.
镉(Cd)是一种有毒的重金属,通常与哺乳动物的生殖障碍有关。食用燕窝(EBN)是一种由燕窝(Aerodramus fuci-phagus & Aerodramus maximus)的唾液制成的天然食品,几十年来一直被作为健康食品或补品食用。本研究旨在探讨EBN对sd大鼠卵巢Cd毒性的保护作用。雌性Sprague Dawley大鼠30只,随机分为5组:1组(阴性对照)给予蒸馏水;2组阳性对照(PC)给予cdcl2, 5mg/kg BW;ebn1组、ebn2组和ebn3组分别饲喂浓度为60、90和120 mg/kg BW的氯化镉(5mg/kg BW)。治疗4周后,将大鼠安乐死,采集卵巢,用苏木精和伊红(H&E)染色进行组织病理学研究。免疫组化法检测血管内皮生长因子(VEGF)表达,超氧化酶歧化酶(SOD)检测试剂盒检测SOD水平。口服cdcl2而不添加EBN可显著降低卵巢组织中VEGF的免疫组化表达(p<0.05),降低SOD的活性(p<0.05)。卵巢组织病理学改变,包括卵泡囊肿和闭锁卵泡数量显著增加(p<0.05),而仅Cd治疗组卵泡数量减少(p<0.05)。与PC组相比,3种不同剂量的cdcl2和EBN均显著提高了卵巢组织中VEGF的表达(p<0.05),组织形态正常,退行性改变程度低,SOD活性显著升高(p<0.05)。总体而言,研究结果表明,口服剂量率为5 mg/kg BW的Cd会导致卵巢发生显著变化,如VEGF表达程度降低、抗氧化活性降低和组织形态学改变。同时,EBN对卵巢Cd毒性具有显著的保护作用,可能是通过其抗氧化作用。
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引用次数: 0
Effect of Ingredients on the Bacteriological Profile of Ready to Eat Meat Sandwiches 配料对即食肉类三明治细菌学特征的影响
Q3 Veterinary Pub Date : 2021-01-01 DOI: 10.17582/journal.jahp/2021/9.s1.69.75
Mohamed A. Hussein, Samar H. Mohamed, Ahmed E. Tharwat, Abdelsalam E. Hafez, Reham A. Gomaa
| The current study aimed to investigate the bacteriological quality of the ready-to-eat (RTE) meat sandwiches prepared using the meat and treated ingredients viz., green salad and tahini sauce. Two hundred and forty samples of RTE meat sandwiches, vegetable salad, and tahini sauce were used in the study. The washing of vegetable salad for 10 min with water providing 50 ppm chlorine reduced (p < 0.05) the aerobic plate count (APC), Bacillus cereus (B. cereus) and Staphylococcus aureus (S. aureus) count. Similarly, addition of 1% garlic essential oil during formulation of tahini sauce reduced (p < 0.05) APC, B. cereus and S. aureus count. The counts in RTE meat sandwiches after using treated green salad and tahini sauce reduced (p < 0.05) APC count from (5.58 ± 1.21 and 6.54 ± 1.12) to (3.2 ± 0.22 and 4. 89 ± 0.31) log10CFU/g. The counts of B.cereus reduced (p < 0.05) from (2.98 ± 0.11 and 3.34 ± 0.15) to (<2 and 2. 49 ± 0.09) and S.aureus reduced (p < 0.05) from (3.18 ± 0.15 and 3.85 ± 0.13) to (<2 and 2. 87± 0.09) log10CFU/g. These results demonstrated that use of treated vegetable salad (washed by chlorine water) and tahini sauce (containing 1% garlic essential oil) in preparation of RTE meat sandwiches results the improved bacteriological quality of RTE meat sandwiches.
|本研究旨在调查即食(RTE)肉类三明治的细菌质量,该三明治使用肉类和处理过的原料,即青菜沙拉和芝麻酱。研究中使用了240份RTE肉类三明治、蔬菜沙拉和芝麻酱的样本。用含氯50 ppm的水洗涤蔬菜沙拉10 min,使好氧平板计数(APC)、蜡样芽孢杆菌(B. cereus)和金黄色葡萄球菌(S. aureus)计数降低(p < 0.05)。同样,在芝麻酱配制过程中添加1%大蒜精油可降低APC、蜡样芽孢杆菌和金黄色葡萄球菌的数量(p < 0.05)。使用处理过的青菜沙拉和芝麻酱后,RTE肉三明治中APC计数从(5.58±1.21)和(6.54±1.12)降低到(3.2±0.22)和(4)(p < 0.05)。89±0.31)log10CFU/g。蜡样芽孢杆菌计数由(2.98±0.11)和(3.34±0.15)降至(<2和2),差异有统计学意义(p < 0.05)。金黄色葡萄球菌从(3.18±0.15和3.85±0.13)降至(<2和2)(p < 0.05)。87±0.09)log10CFU/g。结果表明,采用经处理的蔬菜沙拉(氯水洗涤)和芝麻酱(含1%大蒜精油)制备RTE肉三明治,可提高RTE肉三明治的细菌学质量。
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引用次数: 0
Seroprevalence of Brucellosis in Camels in District Sibi, Balochistan 俾路支省西比地区骆驼布鲁氏菌病的血清患病率
Q3 Veterinary Pub Date : 2021-01-01 DOI: 10.17582/journal.jahp/2022/10.2.153.157
H. Rahman, Asghar Ali Kamboh, Nazar Ali Korejo, Atta Muhammad Memon, Rashid Ali Korejo, Manzoor Ahmed Chandio
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引用次数: 0
Influence of Microalgae Nannochloropsis oculata on Blood Constituents, Reproductive Performance and Productivity in Hi-Plus Doe Rabbits Under North Sinai Conditions in Egypt 埃及北西奈条件下微藻纳米绿藻对Hi-Plus家兔血液成分、繁殖性能和生产力的影响
Q3 Veterinary Pub Date : 2021-01-01 DOI: 10.17582/journal.jahp/2022/10.2.135.145
Ibrahim Samir Abd El-Hamid, Wafaa Adel Abd Fouda, Hesham Attia Shedeed, Safaa Ali Mostafa, Ahmed Mohamed Elbaz, Salah Abo Bakr, Baliegh Hamdy Mosa, Ali Saber Morsy, Amal Mohamed Hasan, Khamis Refaay Emam
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引用次数: 2
Seroprevalence of Contagious Caprine Pleuropneumonia in Goats from Selected Endemic Areas of Sindh 信德省部分流行地区山羊传染性羊胸膜肺炎的血清患病率
Q3 Veterinary Pub Date : 2021-01-01 DOI: 10.17582/journal.jahp/2023/11.1.56.61
Ghulam Mustafa Solangi, Zaheer Ahmed Nizamani, Mansoor Tariq, Zubair Ahmed Leghari, Asghar Ali Kamboh, Barirah Rehman Talpur
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引用次数: 1
Monitoring the Hemato-Biochemical Values in Clinical Cases of Cattle With Respiratory Disorders 牛呼吸系统疾病临床病例血液生化指标的监测
Q3 Veterinary Pub Date : 2021-01-01 DOI: 10.17582/journal.jahp/2021/9.4.391.397
Heba El-Zahar, H. Gouda, Abbas El-Naggar, Aziza Eissa, S. Gouda
| Respiratory disorders have an economic effect on the dairy industry. The purpose of this study was to di-agnose cattle with respiratory problems, as well as to assess the nature of the lesions. A variety of practical approaches are used to examine respiratory disorders in cattle. The present study involved 55 cows of which 10 cows were clinically healthy and served as the control group. Cows were brought to the clinic for a number of complaints, including in-appetence, cough, dyspnea and nasal discharges. The total leucocytic counts, aspartate aminotransferase activities, and carbon dioxide tension were significantly higher in all diseased cows. The ultrasonographical examination of the lungs demonstrated several comet-tail artifacts in cows affected with pulmonary emphysema. Various degrees of pulmonary consolidation in bronchopneumonic cows were observed. Furthermore, cows with pleurisy showed anechoic pleural effusion with fibrin deposits and an irregular pleural surface. It is concluded that, hematological and biochemical findings are the key indicators of bovine health, and they are more expressive when correlated with the history, clinical symptoms, and ultrasonographic examination of cows with respiratory diseases.
呼吸系统疾病对乳制品行业有经济影响。本研究的目的是诊断患有呼吸系统疾病的牛,并评估病变的性质。各种实用的方法被用来检查牛的呼吸系统疾病。本研究选取55头奶牛,其中10头临床健康奶牛作为对照组。奶牛被带到诊所的原因包括食欲不振、咳嗽、呼吸困难和流鼻水。所有病牛的总白细胞计数、天冬氨酸转氨酶活性和二氧化碳张力均显著升高。肺的超声检查显示几个彗星尾伪影在奶牛感染肺气肿。观察支气管肺炎奶牛不同程度的肺实变。此外,乳牛胸膜炎表现为无回声胸膜积液伴纤维蛋白沉积和不规则胸膜表面。结论:血液学和生化指标是牛健康状况的关键指标,与奶牛呼吸系统疾病的病史、临床症状和超声检查相联系更具有代表性。
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引用次数: 0
Effect of Herbal Marination on The Quality Characteristics of Broiler Meat 草药腌制对肉鸡品质特性的影响
Q3 Veterinary Pub Date : 2021-01-01 DOI: 10.17582/journal.jahp/2022/10.3.287.292
Nur Prabewi, S. Supriyanto, Budi Purwo Widiarso
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引用次数: 0
Effect of Replacing Maize with Boiled Sweet Potato on Performance Characteristics and Expression of Insulin-Like Growth Factor-1 Gene in Pigs 水煮甘薯替代玉米对猪生产性能及胰岛素样生长因子-1基因表达的影响
Q3 Veterinary Pub Date : 2021-01-01 DOI: 10.17582/journal.jahp/2022/10.3.293.302
Adimabua Mike Moemeka, Ufuoma Godstime Sorhue, Sylvanus Ikenna Omeje, L. Bratte, Raphael E Onainor, O. Oghenesuvwe
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引用次数: 0
Impact of Environmental Heat Stress on Ovarian Function of Zebu Cows 环境热应激对瘤牛卵巢功能的影响
Q3 Veterinary Pub Date : 2021-01-01 DOI: 10.17582/journal.jahp/2022/10.4.412.419
N. Wachida, P. Dawuda, I. Ate, P. Rekwot
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引用次数: 0
Effects of Selenium Injection on the Reproductive Performance of Holstein Dairy Cows 硒注射液对荷斯坦奶牛繁殖性能的影响
Q3 Veterinary Pub Date : 2021-01-01 DOI: 10.17582/journal.jahp/2021/9.3.335.341
J. Ahn, Gil Woo Han, I. Nam
but Abstract | This study aimed to determine the effects of selenium (Se) injection on 1) the Se concentration in blood, 2) the body condition score, 3) the number of artificial fertilizations per conception, and 4) the days open of dairy cows. In the conduct of the study, forty-two (42) Holstein cows were divided into control and treatment groups. The treatment groups were classified based on the parity of the dairy cows (2 ∼ 3, 4, 5, and 6 ∼ 7 parity). The control group was fed with basal diet while the treatment groups were fed with basal diet plus 40 mL of Se injection. The results showed that the Se concentration in blood was greater in treatment groups than that in the control group (p<0.05). The average Se concentrations in 2 ∼ 7 parity treatment groups and the control group were 0.24 and 0.16 mg/L, respectively. The effects of Se injection on the number of artificial inseminations per conception and on the days open of dairy cows, on the other hand, resulted to be lower in the treated cows compared to the untreated cows or control group. The average number of artificial inseminations per conception in the 6 ∼ 7 parity treatment group (1.50) lowered by half than the control group (3.67). The treated cows also showed a decrease in days open as compared to the untreated cows. The days open decreased by an average of 13.35 days in the treatment groups (126.96 days) compared to the control group (140.31 days). In conclusion, these results indicate that the injection of Se to dairy cows might affect the parameters of their reproduction such as the number of artificial inseminations per conception and days open.
摘要本研究旨在探讨硒(Se)注射对奶牛血硒浓度、体况评分、单次受精卵次数和开胎天数的影响。在这项研究中,42头荷斯坦奶牛被分为对照组和实验组。根据奶牛胎次(2 ~ 3胎、4胎、5胎和6 ~ 7胎)对处理组进行分类。对照组饲喂基础饲粮,试验组饲喂基础饲粮加硒注射液40 mL。结果表明,各治疗组血硒浓度显著高于对照组(p<0.05)。2 ~ 7胎次处理组和对照组的平均硒浓度分别为0.24和0.16 mg/L。另一方面,硒注射对奶牛每次受精卵的人工授精次数和开胎天数的影响,导致处理奶牛低于未处理奶牛或对照组。6 ~ 7胎次治疗组的每次平均人工授精次数(1.50次)比对照组(3.67次)减少了一半。与未接受治疗的奶牛相比,接受治疗的奶牛的开放天数也有所减少。各处理组的开肠天数(126.96 d)比对照组(140.31 d)平均减少13.35 d。综上所述,奶牛注射硒可能会影响奶牛的繁殖参数,如单胎人工授精次数和开胎天数。
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引用次数: 0
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Journal of Animal Health and Production
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