Pub Date : 2013-07-01DOI: 10.15406/MOJOR.2015.02.00050
J. Sultan, M. Lovell
Introduction Greater trochanteric pain syndrome (GTPS) is a common and disabling condition characterised by pain and tenderness at or around the trochanteric area. Extracorporeal shockwave therapy (ESWT) has been described as a method of treatment. The National Institute for Clinical Excellence (NICE, UK) guidance suggests a possible benefit but with limited evidence. Materials and Methods We retrospectively identified 71 consecutive patients who underwent ESWT for refractory GTPS over a period of 16 months. The diagnosis was made clinically. ESWT was offered to patients with refractory symptoms despite conservative treatment. Telephone interviews were used to collect data including pain scores (0–10), change in symptoms, discomfort of the procedure, and complications. Results Fifty-nine patients (83%) were followed up. The mean time to follow up was 8 months (3 to 19). The mean age was 59 (29 to 88) with 86% females. Two-thirds had improvement in their symptoms with a significant drop of 6 points in their pain score (p Conclusion This study shows an improvement in the symptoms of 67% of patients with refractory GTPS, for up to 15 months, but with frequent relapsed. Further research is required to confirm whether MRI may be useful in selecting patients for ESWT.
{"title":"EXTRACORPOREAL SHOCKWAVE THERAPY FOR REFRACTORY GREATER TROCHANTERIC PAIN SYNDROME","authors":"J. Sultan, M. Lovell","doi":"10.15406/MOJOR.2015.02.00050","DOIUrl":"https://doi.org/10.15406/MOJOR.2015.02.00050","url":null,"abstract":"Introduction Greater trochanteric pain syndrome (GTPS) is a common and disabling condition characterised by pain and tenderness at or around the trochanteric area. Extracorporeal shockwave therapy (ESWT) has been described as a method of treatment. The National Institute for Clinical Excellence (NICE, UK) guidance suggests a possible benefit but with limited evidence. Materials and Methods We retrospectively identified 71 consecutive patients who underwent ESWT for refractory GTPS over a period of 16 months. The diagnosis was made clinically. ESWT was offered to patients with refractory symptoms despite conservative treatment. Telephone interviews were used to collect data including pain scores (0–10), change in symptoms, discomfort of the procedure, and complications. Results Fifty-nine patients (83%) were followed up. The mean time to follow up was 8 months (3 to 19). The mean age was 59 (29 to 88) with 86% females. Two-thirds had improvement in their symptoms with a significant drop of 6 points in their pain score (p Conclusion This study shows an improvement in the symptoms of 67% of patients with refractory GTPS, for up to 15 months, but with frequent relapsed. Further research is required to confirm whether MRI may be useful in selecting patients for ESWT.","PeriodicalId":15048,"journal":{"name":"Journal of Bone and Joint Surgery-british Volume","volume":"114 1","pages":"11-11"},"PeriodicalIF":0.0,"publicationDate":"2013-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80768265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-07-01DOI: 10.1302/0301-620X.95B7.32485
F. Haddad
I would like to begin with a big thank you to Ricky Villar, the outgoing Editor-in-Chief, who has brought tremendous energy to the leadership of The Bone & Joint Journal . This transition has come about slightly quicker than Ricky, myself or anybody else expected. This is a reflection of his
{"title":"Core values, new beginning","authors":"F. Haddad","doi":"10.1302/0301-620X.95B7.32485","DOIUrl":"https://doi.org/10.1302/0301-620X.95B7.32485","url":null,"abstract":"I would like to begin with a big thank you to Ricky Villar, the outgoing Editor-in-Chief, who has brought tremendous energy to the leadership of The Bone & Joint Journal . This transition has come about slightly quicker than Ricky, myself or anybody else expected. This is a reflection of his","PeriodicalId":15048,"journal":{"name":"Journal of Bone and Joint Surgery-british Volume","volume":"30 1","pages":"865-865"},"PeriodicalIF":0.0,"publicationDate":"2013-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82093134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Deschepper, J. Paquet, M. Manassero, D. Logeart-Avramoglou, M. Bensidhoum, H. Petite
Summary In this study, we challenged the current paradigm of human Mesenchymal Stem Cells survival, which assigned a pivotal role to oxygen, by testing the hypothesis that exogenous glucose may be key to their survival. Introduction The survival of human mesenchymal stem cells (hMSCs) has elicited a great deal of interest, because it is relevant to the efficacy of engineered tissues. However, to date, hMSCs have not met this promise, in part due to the high death rate of cells upon transplantation. In this study, we challenged the current paradigm of hMSC survival, which assigned a pivotal role to oxygen, by testing the hypothesis that exogenous glucose may be key to hMSC survival. Materials and methods In vitro model of ischemia 2.10 4 hMSCs from five donors, were seeded into individual wells of a 24-well plate, cultured overnight, washed twice with PBS and then maintained in hypoxia (0.1% oxygen) under serum (FBS) free αMEM medium in either the absence or in the presence (1 or 5 g/L) of glucose for 21 days. In vitro Cell viability: To assess the role of glucose on hMSCs viability, cells were cultured under hypoxia in the absence or in the presence of glucose (1 and 5g/L), At days 0, 3, 7, 14 and 21, cell viability was evaluated by flow cytometry and ATP content per cell quantified. In vivo effect of glucose supply on hMSCs viability 3.10 5 eGFG-luc hMSCs were seeded on a cylindrical AN-69 scaffolds. At the time of implantation, 100 µl of hyaluronic acide (HA) (2%) containing either 0g/L (negative control) or 10g/L of glucose was gently injected inside the construct. Cell- constructs were implanted subcutaneously in eight week-old mice (2 per animal) and were imaged by bioluminescence imaging (BLI) at day 1, 4, 7 and 14 until sacrifice. Results hMSCs were able to survive and to maintain their ATP content 21 days under sustained hypoxia providing that they were cultured in the presence of a sufficient glucose supply (i.e. 5g/L). In contrast, hMSCs cultured without or with 1g/L of glucose failed to survive. These results established that glucose depletion but not sustained hypoxia affected cell survival. In vivo results showed a striking increase of cell viability in cell constructs loaded with glucose. At day 14, a five-fold increase in cell number was observed in cell constructs loaded with glucose when compared to the control cell constructs without glucose. Discussion The present study challenge the current paradigm that gives a pivotal role to oxygen on hMSCs massive cell death. By using an in vitro model of hypoxia/ischemia, we demonstrated that in the presence of sufficient glucose, hMSCs were able to survive 21 days under sustained hypoxia. Most importantly, an appropriate glucose supply strongly increases cell viability of hMSCs implanted subcutaneously in a mice model. This study provides evidences that glucose depletion but not hypoxia affects hMSCs viability. Further investigations need to be performed to develop hydrogels that ensure
{"title":"PRO-ANGIOGENIC AND PRO-SURVIVAL FUNCTIONS OF GLUCOSE IN HUMAN MESENCHYMAL STEM CELLS UPON TRANSPLANTATION","authors":"M. Deschepper, J. Paquet, M. Manassero, D. Logeart-Avramoglou, M. Bensidhoum, H. Petite","doi":"10.1530/BONEABS.1.PP114","DOIUrl":"https://doi.org/10.1530/BONEABS.1.PP114","url":null,"abstract":"Summary In this study, we challenged the current paradigm of human Mesenchymal Stem Cells survival, which assigned a pivotal role to oxygen, by testing the hypothesis that exogenous glucose may be key to their survival. Introduction The survival of human mesenchymal stem cells (hMSCs) has elicited a great deal of interest, because it is relevant to the efficacy of engineered tissues. However, to date, hMSCs have not met this promise, in part due to the high death rate of cells upon transplantation. In this study, we challenged the current paradigm of hMSC survival, which assigned a pivotal role to oxygen, by testing the hypothesis that exogenous glucose may be key to hMSC survival. Materials and methods In vitro model of ischemia 2.10 4 hMSCs from five donors, were seeded into individual wells of a 24-well plate, cultured overnight, washed twice with PBS and then maintained in hypoxia (0.1% oxygen) under serum (FBS) free αMEM medium in either the absence or in the presence (1 or 5 g/L) of glucose for 21 days. In vitro Cell viability: To assess the role of glucose on hMSCs viability, cells were cultured under hypoxia in the absence or in the presence of glucose (1 and 5g/L), At days 0, 3, 7, 14 and 21, cell viability was evaluated by flow cytometry and ATP content per cell quantified. In vivo effect of glucose supply on hMSCs viability 3.10 5 eGFG-luc hMSCs were seeded on a cylindrical AN-69 scaffolds. At the time of implantation, 100 µl of hyaluronic acide (HA) (2%) containing either 0g/L (negative control) or 10g/L of glucose was gently injected inside the construct. Cell- constructs were implanted subcutaneously in eight week-old mice (2 per animal) and were imaged by bioluminescence imaging (BLI) at day 1, 4, 7 and 14 until sacrifice. Results hMSCs were able to survive and to maintain their ATP content 21 days under sustained hypoxia providing that they were cultured in the presence of a sufficient glucose supply (i.e. 5g/L). In contrast, hMSCs cultured without or with 1g/L of glucose failed to survive. These results established that glucose depletion but not sustained hypoxia affected cell survival. In vivo results showed a striking increase of cell viability in cell constructs loaded with glucose. At day 14, a five-fold increase in cell number was observed in cell constructs loaded with glucose when compared to the control cell constructs without glucose. Discussion The present study challenge the current paradigm that gives a pivotal role to oxygen on hMSCs massive cell death. By using an in vitro model of hypoxia/ischemia, we demonstrated that in the presence of sufficient glucose, hMSCs were able to survive 21 days under sustained hypoxia. Most importantly, an appropriate glucose supply strongly increases cell viability of hMSCs implanted subcutaneously in a mice model. This study provides evidences that glucose depletion but not hypoxia affects hMSCs viability. Further investigations need to be performed to develop hydrogels that ensure ","PeriodicalId":15048,"journal":{"name":"Journal of Bone and Joint Surgery-british Volume","volume":"5 1","pages":"238-238"},"PeriodicalIF":0.0,"publicationDate":"2013-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91033649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-04-01DOI: 10.1302/0301-620X.95B4.31902
V. Khanduja
{"title":"Exam Corner – April 2013","authors":"V. Khanduja","doi":"10.1302/0301-620X.95B4.31902","DOIUrl":"https://doi.org/10.1302/0301-620X.95B4.31902","url":null,"abstract":"","PeriodicalId":15048,"journal":{"name":"Journal of Bone and Joint Surgery-british Volume","volume":"48 1","pages":"575-576"},"PeriodicalIF":0.0,"publicationDate":"2013-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87614781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-03-01DOI: 10.1016/J.IJSU.2012.06.287
L. Athanatos, N. Nixon, G. Holmes, L. James, A. Bass
{"title":"OUTCOME MEASURES IN FLAT FOOT: ANALYSIS OF PEDOBAROGRAPHIC AND RADIOGRAPHIC TECHNIQUES","authors":"L. Athanatos, N. Nixon, G. Holmes, L. James, A. Bass","doi":"10.1016/J.IJSU.2012.06.287","DOIUrl":"https://doi.org/10.1016/J.IJSU.2012.06.287","url":null,"abstract":"","PeriodicalId":15048,"journal":{"name":"Journal of Bone and Joint Surgery-british Volume","volume":"61 1","pages":"16-16"},"PeriodicalIF":0.0,"publicationDate":"2013-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89492323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-03-01DOI: 10.1302/0301-620X.95B3.31744
V. Khanduja
{"title":"Exam Corner – March 2013","authors":"V. Khanduja","doi":"10.1302/0301-620X.95B3.31744","DOIUrl":"https://doi.org/10.1302/0301-620X.95B3.31744","url":null,"abstract":"","PeriodicalId":15048,"journal":{"name":"Journal of Bone and Joint Surgery-british Volume","volume":"368 1","pages":"431-432"},"PeriodicalIF":0.0,"publicationDate":"2013-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80402660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-03-01DOI: 10.1007/978-3-642-45266-6_3
Y. Cho, Joo Hyun Lee, Eun Seong Sagong
{"title":"Comparison of Linear Wear Rate According to Femoral Head Sizes in Metal on Conventional UHMWPE Liner","authors":"Y. Cho, Joo Hyun Lee, Eun Seong Sagong","doi":"10.1007/978-3-642-45266-6_3","DOIUrl":"https://doi.org/10.1007/978-3-642-45266-6_3","url":null,"abstract":"","PeriodicalId":15048,"journal":{"name":"Journal of Bone and Joint Surgery-british Volume","volume":"81 6 1","pages":"27-33"},"PeriodicalIF":0.0,"publicationDate":"2013-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77390217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The objective of this study is to investigate the effect of the tensile force ratio between the two extensor muscles for the hip joint on the forces acting on the knee joint. We have created a mathematical model of lower limb and have performed some simulations to introduce the forces acting on the knee joint for various daily activities. With only one exception, our results for knee joint forces were in good or close agreement involving all range of knee flexion either with the in vivo data or other literature data. The exception was that, at high knee flexion angle (knee bend), the tangential components of knee joint force became pretty larger than those from the in vivo data, while the normal components did not differ much with each other though as shown in Fig. 1. We considered that the above mentioned discrepancy was attributed to the fact that in order to solve an indeterminate problem, we had assumed the hamstrings and the gluteus maximus work together with the same force with each other, thereby introducing the hamstrings force too great. Then we expected that the above discrepancy could be eliminated if we change the tensile force ratio between the hamstrings and the gluteus maximus basing upon a certain biomechanical criterion, for example the biological cross-sectional areas. Thus we modified our model so that we could introduce the knee joint forces as a function of the tensile force ratio. Simulation was performed for the various tensile ratio values and it was found that the knee joint force was sensitively affected by the tensile ratio and the above mentioned discrepancy between the simulation results and the in vivo data could be eliminated if the ratio value was appropriately chosen. Figure 2 shows the situation; Variations of F n and F t as a function of knee angle q for the various tensile force ratio r between the hamstrings and the gluteus maximus. Where, r=1.56 was determined from the biological cross-sectional areas of the hamstrings and the gluteus maximus and r=4.5 was determined so that the simulation results best fit to the in vivo data. It has been criticized that there exist large variations of knee joint forces obtained from model analyses. And the reasons for this have been attributed to for example such facts that the model is 2D and the parameter values are incorrect. Yet, another important issue may be to find out the way how to determine the value of the synergetic muscles9 force ratio with reflecting a biological rationality.
{"title":"Effect of the Tensile Force Ratio of Gluteus Maximus and Hamstrings on the Knee Joint Force","authors":"S. Hirokawa, Michihiko Fukunaga, M. Mawatari","doi":"10.1299/KIKAIC.78.1192","DOIUrl":"https://doi.org/10.1299/KIKAIC.78.1192","url":null,"abstract":"The objective of this study is to investigate the effect of the tensile force ratio between the two extensor muscles for the hip joint on the forces acting on the knee joint. We have created a mathematical model of lower limb and have performed some simulations to introduce the forces acting on the knee joint for various daily activities. With only one exception, our results for knee joint forces were in good or close agreement involving all range of knee flexion either with the in vivo data or other literature data. The exception was that, at high knee flexion angle (knee bend), the tangential components of knee joint force became pretty larger than those from the in vivo data, while the normal components did not differ much with each other though as shown in Fig. 1. We considered that the above mentioned discrepancy was attributed to the fact that in order to solve an indeterminate problem, we had assumed the hamstrings and the gluteus maximus work together with the same force with each other, thereby introducing the hamstrings force too great. Then we expected that the above discrepancy could be eliminated if we change the tensile force ratio between the hamstrings and the gluteus maximus basing upon a certain biomechanical criterion, for example the biological cross-sectional areas. Thus we modified our model so that we could introduce the knee joint forces as a function of the tensile force ratio. Simulation was performed for the various tensile ratio values and it was found that the knee joint force was sensitively affected by the tensile ratio and the above mentioned discrepancy between the simulation results and the in vivo data could be eliminated if the ratio value was appropriately chosen. Figure 2 shows the situation; Variations of F n and F t as a function of knee angle q for the various tensile force ratio r between the hamstrings and the gluteus maximus. Where, r=1.56 was determined from the biological cross-sectional areas of the hamstrings and the gluteus maximus and r=4.5 was determined so that the simulation results best fit to the in vivo data. It has been criticized that there exist large variations of knee joint forces obtained from model analyses. And the reasons for this have been attributed to for example such facts that the model is 2D and the parameter values are incorrect. Yet, another important issue may be to find out the way how to determine the value of the synergetic muscles9 force ratio with reflecting a biological rationality.","PeriodicalId":15048,"journal":{"name":"Journal of Bone and Joint Surgery-british Volume","volume":"119 4 1","pages":"198-198"},"PeriodicalIF":0.0,"publicationDate":"2013-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88751974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Introduction Precision error (PE) in Dual Energy X-Ray Absorptiometry (DXA) is important for accurate monitoring of changes in Bone-Mineral-Density (BMD). It has been demonstrated that BMD PE increases with increasing BMI. In vivo PE for the Trabecular-Bone-Score (TBS) has not been reported. This study aimed to evaluate the short-term PE (STPE)) of BMD and TBS and to investigate the effect of obesity on DXA PE. Method DXA lumbar spine scans (L1–L4) were performed using GE Lunar Prodigy. STPE was measured in 91 women (Group A) at a single visit by duplicating scans with repositioning in-between. PE was calculated as the percentage coefficient of variation (%CV). Group A was sub-divided into four groups based on BMI (A.1. 35kg/m2) to assess the effect of obesity on STPE. Abnormally different vertebrae were excluded from the analysis in accordance with The International Society for Clinical Densitometry (ISCD) recommendations. Results The Group A STPE was 1.26 % for BMD and 2.04% for TBS. Short-term PE for BMD and TBS respectively in the BMI subgroups was: A.1. 1.07% and 1.82%, A.2. 1.34% and 2.26%, A.3. 1.25% and 2.35%, A.4. 1.68% and 1.82%. Conclusion The results show that STPE is higher for TBS than for BMD. Short-term PE for both BMD and TBS are adversely affected by increasing BMI but this effect is mitigated in the highest BMI category where use of the ‘thick’ scanning mode improves signal to noise ratio.
{"title":"SHORT-TERM PRECISION ERROR IN DUAL ENERGY X-RAY ABSORPTIOMETRY BONE-MINERAL-DENSITY AND TRABECULAR-BONE-SCORE MEASUREMENTS; AND EFFECTS OF OBESITY","authors":"S. Hopkins, K. Knapp, D. Parker, R. Yusof","doi":"10.5430/JBGC.V4N2P8","DOIUrl":"https://doi.org/10.5430/JBGC.V4N2P8","url":null,"abstract":"Introduction Precision error (PE) in Dual Energy X-Ray Absorptiometry (DXA) is important for accurate monitoring of changes in Bone-Mineral-Density (BMD). It has been demonstrated that BMD PE increases with increasing BMI. In vivo PE for the Trabecular-Bone-Score (TBS) has not been reported. This study aimed to evaluate the short-term PE (STPE)) of BMD and TBS and to investigate the effect of obesity on DXA PE. Method DXA lumbar spine scans (L1–L4) were performed using GE Lunar Prodigy. STPE was measured in 91 women (Group A) at a single visit by duplicating scans with repositioning in-between. PE was calculated as the percentage coefficient of variation (%CV). Group A was sub-divided into four groups based on BMI (A.1. 35kg/m2) to assess the effect of obesity on STPE. Abnormally different vertebrae were excluded from the analysis in accordance with The International Society for Clinical Densitometry (ISCD) recommendations. Results The Group A STPE was 1.26 % for BMD and 2.04% for TBS. Short-term PE for BMD and TBS respectively in the BMI subgroups was: A.1. 1.07% and 1.82%, A.2. 1.34% and 2.26%, A.3. 1.25% and 2.35%, A.4. 1.68% and 1.82%. Conclusion The results show that STPE is higher for TBS than for BMD. Short-term PE for both BMD and TBS are adversely affected by increasing BMI but this effect is mitigated in the highest BMI category where use of the ‘thick’ scanning mode improves signal to noise ratio.","PeriodicalId":15048,"journal":{"name":"Journal of Bone and Joint Surgery-british Volume","volume":"35 1","pages":"54-54"},"PeriodicalIF":0.0,"publicationDate":"2013-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74110423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-02-08DOI: 10.3760/CMA.J.ISSN.1001-9030.2013.02.053
Yue Ding, Zhong-ning Guan, Jie Xu, Ruofan Ma
Summary Osteoporosis reduces particle-induced osteolysis in rat model. Introduction Wear particle induced osteolysis is considered to be a vital factor that reduces the life span of joint prosthesis. Osteoporosis is not rare in patients with indication for arthroplasty. However, the influence of osteoporosis on wear particles induced osteolysis is not clear. This study is aimed to explore on this issue by using animal model. Methods 42 female Sprague-Dawley (SD) rats aged 6 months were randomly divided into 3 groups: A, B and C group. Group A and B contained 18 rats each, and group C contained 6 rats. The rats in group A underwent bilateral ovariectomy. Group B was normal control, and group C was sham control. After 3 months, 6 rats in group A, 6 rats in group B and all the rats of group C were sacrificed. Bone mineral density (BMD), μCT and bone histomorphometry were conducted. The rest of rats in group A were randomly divided into 2 groups: group A1 and group A2, and so were the rats in group B. 5mg titanium particles were implanted onto the calvaria of groups A1 and B1, and isometric PBS solution were injected to group A2 and B2. Calvaria were harvested after 14 days. Calvaria were analyzed by μCT and histomorphometry to measure the osteolysis area of calvarial sagittal suture. Results Compared with B and C group, BMD and bone histomorphometry index of group A was significantly reduced ( P <0.05), and tibial trabeculae of group A was slimmer. Area of calvarial sagittal suture osteolysis were 0.262±0.009mm2, 0.130±0.013mm2, 0.307±0.013mm2 and 0.178±0.011mm2 in A1, A2, B1and B2 groups, respectively. There was significant difference among the groups. Conclusions Osteoporosis may reduce particle-induced osteolysis in rat model.
{"title":"THE INFLUENCE OF OSTEOPOROSIS ON WEAR PARTICLES INDUCED OSTEOLYSIS IN ANIMAL MODEL","authors":"Yue Ding, Zhong-ning Guan, Jie Xu, Ruofan Ma","doi":"10.3760/CMA.J.ISSN.1001-9030.2013.02.053","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1001-9030.2013.02.053","url":null,"abstract":"Summary Osteoporosis reduces particle-induced osteolysis in rat model.\u0000\u0000Introduction Wear particle induced osteolysis is considered to be a vital factor that reduces the life span of joint prosthesis. Osteoporosis is not rare in patients with indication for arthroplasty. However, the influence of osteoporosis on wear particles induced osteolysis is not clear. This study is aimed to explore on this issue by using animal model.\u0000\u0000Methods 42 female Sprague-Dawley (SD) rats aged 6 months were randomly divided into 3 groups: A, B and C group. Group A and B contained 18 rats each, and group C contained 6 rats. The rats in group A underwent bilateral ovariectomy. Group B was normal control, and group C was sham control. After 3 months, 6 rats in group A, 6 rats in group B and all the rats of group C were sacrificed. Bone mineral density (BMD), μCT and bone histomorphometry were conducted. The rest of rats in group A were randomly divided into 2 groups: group A1 and group A2, and so were the rats in group B. 5mg titanium particles were implanted onto the calvaria of groups A1 and B1, and isometric PBS solution were injected to group A2 and B2. Calvaria were harvested after 14 days. Calvaria were analyzed by μCT and histomorphometry to measure the osteolysis area of calvarial sagittal suture.\u0000\u0000Results Compared with B and C group, BMD and bone histomorphometry index of group A was significantly reduced ( P <0.05), and tibial trabeculae of group A was slimmer. Area of calvarial sagittal suture osteolysis were 0.262±0.009mm2, 0.130±0.013mm2, 0.307±0.013mm2 and 0.178±0.011mm2 in A1, A2, B1and B2 groups, respectively. There was significant difference among the groups.\u0000\u0000Conclusions Osteoporosis may reduce particle-induced osteolysis in rat model.","PeriodicalId":15048,"journal":{"name":"Journal of Bone and Joint Surgery-british Volume","volume":"11 1","pages":"40-40"},"PeriodicalIF":0.0,"publicationDate":"2013-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90286303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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