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Journal of Bioprocess Engineering and Biorefinery最新文献

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Optimizing Hardwood Prehydrolysis for Simultaneous Production of Bioproducts and Biomaterials 同时生产生物制品和生物材料的硬木预水解优化
Pub Date : 2012-06-01 DOI: 10.1166/JBEB.2012.1006
T. Radiotis, Xiao Zhang, M. Paice, V. Byrne
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引用次数: 6
Processes and Bioreactor Designs for Butanol Production from Lignocellulosic Biomass 木质纤维素生物质生产丁醇的工艺和生物反应器设计
Pub Date : 2012-06-01 DOI: 10.1166/JBEB.2012.1009
J. P. Buyondo, Shijie Liu
Butanol is a chemical, solvent and a good liquid fuel, which can be produced from lignocellulosic biomass. This paper provides a review on the processes involved in butanol production from lignocellulosic biomass. Processes reviewed include: metabolic pathways of most commonly used microorganisms, lignocellulosic biomass as a potential feedstock for butanol production and bioreactor designs for batch and continuous butanol production. Butanol production from lignocellulosic biomass via different bioreactor designs is compared in terms of butanol concentration, butanol yield and butanol productivity. Factors affecting the fermentative butanol production are summarized and discussed: microbial inhibitors, acetate addition, butyrate addition, hydrogen production, culture stability, anaerobic conditions, agitation, pH, and temperature.
丁醇是一种化学品、溶剂和良好的液体燃料,可以从木质纤维素生物质中生产。本文综述了木质纤维素生物质生产丁醇的研究进展。回顾的过程包括:最常用微生物的代谢途径,木质纤维素生物质作为丁醇生产的潜在原料,以及分批和连续丁醇生产的生物反应器设计。通过不同的生物反应器设计,从木质纤维素生物质中生产丁醇,比较了丁醇浓度、丁醇产量和丁醇生产率。总结并讨论了影响发酵丁醇生产的因素:微生物抑制剂、乙酸添加、丁酸添加、产氢、培养稳定性、厌氧条件、搅拌、pH和温度。
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引用次数: 1
The Effect of Co-Substrate Feeding on Polyhydroxyalkanoate (PHA) Homopolymer and Copolymer Production in Recombinant Escherichia coli LS5218 共底物进料对重组大肠杆菌LS5218聚羟基烷酸酯(PHA)均聚物及共聚物产量的影响
Pub Date : 2012-06-01 DOI: 10.1166/JBEB.2012.1003
Qin Wang, Chengjun Zhu, Thomas J. Yancone, C. Nomura
Polyhydroxyalkanoates (PHAs) are aliphatic polyesters produced by a wide variety of bacteria as carbon and energy storage sources. Copolymers of the short chain length (SCL) 3-hydroxybutyrate (3HB) repeating unit and medium chain length hydroxyalkanoate (MCL-HA) repeating units have better thermal and mechanical properties than poly-3HB homopolymers. In this study, a MCL monomer supplier gene phaJ4 from Pseudomonas putida KT2440, an engineered PHA synthase gene (phaC1) from Pseudomonas sp. 61-3, and the SCL monomer supplier genes, phaA and phaB from Ralstonia eutropha, were coexpressed under the lac promoter in recombinant E. coli LS5218 to produce SCL-MCL copolymers. The recombinant strains were co-fed lauric acid (a fatty acid) and glucose as a carbon source and the ratio of SCL to MCL monomers varied dependant on differences in the timing of the addition of each carbon source during the fermentation. The PHA copolymers produced exhibited a range from 0.4 mol% to 35 mol% MCL repeating units giving rise to wide range of physical properties. The molecular weights and thermal properties of different polymers were studied by gel permeation chromatography (GPC) and differential scanning calorimetry (DSC). The results suggested the polymers produced by co-feeding with lauric acid and glucose were mixtures of 3HB homopolymers and 3HB-co-MCL copolymers. This conclusion was confirmed by acetone fractionation.
聚羟基烷酸酯(PHAs)是由多种细菌产生的脂肪族聚酯,作为碳和能量的储存来源。短链长度(SCL) 3-羟基丁酸酯(3HB)重复单元和中链长度羟基烷酸酯(MCL-HA)重复单元的共聚物比聚3HB均聚物具有更好的热性能和力学性能。本研究将来自恶臭假单胞菌KT2440的MCL单体供给基因phaJ4、来自假单胞菌sp. 61-3的PHA合成酶工程基因phaac1和来自富营养菌Ralstonia的SCL单体供给基因phaA和phaB在lac启动子下在重组大肠杆菌LS5218中共表达,制备了SCL-MCL共聚物。重组菌株以月桂酸(一种脂肪酸)和葡萄糖为碳源,发酵过程中SCL与MCL单体的比例随每种碳源添加时间的不同而变化。所制得的PHA共聚物具有0.4 ~ 35 mol%的MCL重复单元,具有广泛的物理性质。采用凝胶渗透色谱法(GPC)和差示扫描量热法(DSC)研究了不同聚合物的分子量和热性能。结果表明,与月桂酸和葡萄糖共投料制得的聚合物是3HB均聚物和3HB-co- mcl共聚物的混合物。丙酮分馏证实了这一结论。
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引用次数: 4
Catalytic Conversion of Glucose Into Fuels and Chemicals via 5-Hydroxymethylfurfural 5-羟甲基糠醛催化葡萄糖转化为燃料和化学品
Pub Date : 2012-06-01 DOI: 10.1166/JBEB.2012.1002
Lei Hu, Geng Zhao, Yong Sun, Lu Lin, Shijie Liu
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引用次数: 2
Woody Biomass Conversion: Sustainability and Water-Based Processes 木质生物质转化:可持续性和水基过程
Pub Date : 2012-06-01 DOI: 10.1166/JBEB.2012.1008
Shijie Liu
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引用次数: 7
Ethanol Production via In-Situ Detoxification of Spent Sulfite Liquor 亚硫酸盐废液原位解毒生产乙醇
Pub Date : 2012-06-01 DOI: 10.1166/JBEB.2012.1011
Shiho Takahashi, K. Shiell, P. Fatehi, Y. Ni
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引用次数: 8
Butadiene Production from Ethanol 乙醇生产丁二烯
Pub Date : 2012-06-01 DOI: 10.1166/JBEB.2012.1010
Yang Wang, Shijie Liu
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引用次数: 15
Optimization of a Poly-3-hydroxybutyrate Quantification Method for Rapid Detection in Plant Based Systems 植物基体系中聚3-羟基丁酸酯快速检测方法的优化
Pub Date : 2012-06-01 DOI: 10.1166/JBEB.2012.1013
William Hohenschuh, Cathleen Ma, D. A. Dalton, G. Murthy
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引用次数: 2
Welcome to the Journal of Bioprocess Engineering and Biorefinery 欢迎来到《生物过程工程与生物炼制》杂志
Pub Date : 2012-06-01 DOI: 10.1166/JBEB.2012.1001
Shijie Liu
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引用次数: 2
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Journal of Bioprocess Engineering and Biorefinery
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