Pub Date : 2024-05-29DOI: 10.1007/s10886-024-01511-z
Adriel M Sierra, Omayra Meléndez, Rita Bethancourt, Ariadna Bethancourt, Lilisbeth Rodríguez-Castro, Christian A López, Brian E Sedio, Kristin Saltonstall, Juan Carlos Villarreal A
Plant-microbe interactions play a pivotal role in shaping host fitness, especially concerning chemical defense mechanisms. In cycads, establishing direct correlations between specific endophytic microbes and the synthesis of highly toxic defensive phytochemicals has been challenging. Our research delves into the intricate relationship between plant-microbe associations and the variation of secondary metabolite production in two closely related Zamia species that grow in distinct habitats; terrestrial and epiphytic. Employing an integrated approach, we combined microbial metabarcoding, which characterize the leaf endophytic bacterial and fungal communities, with untargeted metabolomics to test if the relative abundances of specific microbial taxa in these two Zamia species were associated with different metabolome profiles. The two species studied shared approximately 90% of the metabolites spanning diverse biosynthetic pathways: alkaloids, amino acids, carbohydrates, fatty acids, polyketides, shikimates, phenylpropanoids, and terpenoids. Co-occurrence networks revealed positive associations among metabolites from different pathways, underscoring the complexity of their interactions. Our integrated analysis demonstrated to some degree that the intraspecific variation in metabolome profiles of the two host species was associated with the abundance of bacterial orders Acidobacteriales and Frankiales, as well as the fungal endophytes belonging to the orders Chaetothyriales, Glomerellales, Heliotiales, Hypocreales, and Sordariales. We further associate individual metabolic similarity with four specific fungal endophyte members of the core microbiota, but no specific bacterial taxa associations were identified. This study represents a pioneering investigation to characterize leaf endophytes and their association with metabolomes in tropical gymnosperms, laying the groundwork for deeper inquiries into this complex domain.
{"title":"Leaf Endophytes Relationship with Host Metabolome Expression in Tropical Gymnosperms.","authors":"Adriel M Sierra, Omayra Meléndez, Rita Bethancourt, Ariadna Bethancourt, Lilisbeth Rodríguez-Castro, Christian A López, Brian E Sedio, Kristin Saltonstall, Juan Carlos Villarreal A","doi":"10.1007/s10886-024-01511-z","DOIUrl":"https://doi.org/10.1007/s10886-024-01511-z","url":null,"abstract":"<p><p>Plant-microbe interactions play a pivotal role in shaping host fitness, especially concerning chemical defense mechanisms. In cycads, establishing direct correlations between specific endophytic microbes and the synthesis of highly toxic defensive phytochemicals has been challenging. Our research delves into the intricate relationship between plant-microbe associations and the variation of secondary metabolite production in two closely related Zamia species that grow in distinct habitats; terrestrial and epiphytic. Employing an integrated approach, we combined microbial metabarcoding, which characterize the leaf endophytic bacterial and fungal communities, with untargeted metabolomics to test if the relative abundances of specific microbial taxa in these two Zamia species were associated with different metabolome profiles. The two species studied shared approximately 90% of the metabolites spanning diverse biosynthetic pathways: alkaloids, amino acids, carbohydrates, fatty acids, polyketides, shikimates, phenylpropanoids, and terpenoids. Co-occurrence networks revealed positive associations among metabolites from different pathways, underscoring the complexity of their interactions. Our integrated analysis demonstrated to some degree that the intraspecific variation in metabolome profiles of the two host species was associated with the abundance of bacterial orders Acidobacteriales and Frankiales, as well as the fungal endophytes belonging to the orders Chaetothyriales, Glomerellales, Heliotiales, Hypocreales, and Sordariales. We further associate individual metabolic similarity with four specific fungal endophyte members of the core microbiota, but no specific bacterial taxa associations were identified. This study represents a pioneering investigation to characterize leaf endophytes and their association with metabolomes in tropical gymnosperms, laying the groundwork for deeper inquiries into this complex domain.</p>","PeriodicalId":15346,"journal":{"name":"Journal of Chemical Ecology","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141160815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-29DOI: 10.1007/s10886-024-01502-0
Emma M Kovacs, Charlotte Pinard, Regine Gries, Arshpreet Manku, Gerhard Gries
Stable flies, Stomoxys calcitrans (L.), are blood-feeding ectoparasites of cattle. Host-seeking stable flies respond to various cattle host cues, but a potential role of cattle breath gases [carbon dioxide (CO2), methane (CH4)] and cattle breath volatiles (acetone, isoprene, 2-butanone, 2-propanol, propionic acid, 3-methyl butyric acid, phenol), alone or in combination, on host-seeking behavior of stable flies has not yet been comprehensively investigated. In laboratory and greenhouse experiments, we tested the hypotheses that (1) CO2 and CH4 interactively attract stable flies, (2) CO2 'gates' attraction of stable flies to CH4, and (3) breath volatiles on their own, or in combination with both CO2 and CH4, attract stable flies. In Y-tube olfactometer experiments, the blend of CH4 (0.5%) and CO2 (1%) in breathing air ('b-air') attracted significantly more female flies than CH4, or CO2, in b-air. The flies' responses to CH4 were contingent upon their prior or concurrent exposure to CO2. In two-choice experiments in a large greenhouse compartment, significantly more flies landed on the host-look-alike barrel that disseminated a blend of CO2 and CH4 in b-air (CO2/CH4/b-air) than on the barrel disseminating either b-air or CO2. Moreover, significantly more flies landed on the barrel that disseminated synthetic breath volatiles (SBVs) than on the barrel disseminating b-air. The blend of CO2/CH4/b-air and SBVs elicited more fly landings on barrels than CO2/CH4/b-air but not than SBVs. SBVs, possibly combined with both CH4 and CO2, could be developed as a lure to enhance trap captures of stable flies in livestock production facilities.
稳定蝇(Stomoxys calcitrans (L.))是牛的食血外寄生虫。寻找宿主的厩蝇会对各种牛宿主线索做出反应,但牛呼吸气体[二氧化碳(CO2)、甲烷(CH4)]和牛呼吸挥发物(丙酮、异戊二烯、2-丁酮、2-丙醇、丙酸、3-甲基丁酸、苯酚)单独或共同对厩蝇寻找宿主行为的潜在作用尚未得到全面研究。在实验室和温室实验中,我们检验了以下假设:(1) CO2 和 CH4 相互吸引稳定蝇;(2) CO2 "门 "吸引稳定蝇到 CH4;(3) 呼吸挥发物本身或与 CO2 和 CH4 结合吸引稳定蝇。在 Y 型管嗅觉仪实验中,呼吸空气("b-air")中混合 CH4(0.5%)和 CO2(1%)吸引的雌蝇明显多于 b-air中的 CH4 或 CO2。苍蝇对 CH4 的反应取决于它们之前或同时接触 CO2 的情况。在一个大型温室中进行的二选一实验中,落在散发二氧化碳和甲烷混合气体(CO2/CH4/b-air)的类似宿主桶上的苍蝇明显多于落在散发b-air或二氧化碳的桶上的苍蝇。此外,落在散发合成呼吸挥发物(SBVs)的木桶上的苍蝇明显多于散发 b-空气的木桶。CO2/CH4/b-air 与 SBVs 的混合比 CO2/CH4/b-air 更能吸引苍蝇落在桶上,但不比 SBVs 更能吸引苍蝇落在桶上。SBV(可能与 CH4 和 CO2 混合使用)可作为一种诱饵,用于提高家畜生产设施中捕获厩蝇的能力。
{"title":"Carbon Dioxide, Methane, and Synthetic Cattle Breath Volatiles Attract Host-Seeking Stable Flies, Stomoxys calcitrans.","authors":"Emma M Kovacs, Charlotte Pinard, Regine Gries, Arshpreet Manku, Gerhard Gries","doi":"10.1007/s10886-024-01502-0","DOIUrl":"https://doi.org/10.1007/s10886-024-01502-0","url":null,"abstract":"<p><p>Stable flies, Stomoxys calcitrans (L.), are blood-feeding ectoparasites of cattle. Host-seeking stable flies respond to various cattle host cues, but a potential role of cattle breath gases [carbon dioxide (CO<sub>2</sub>), methane (CH<sub>4</sub>)] and cattle breath volatiles (acetone, isoprene, 2-butanone, 2-propanol, propionic acid, 3-methyl butyric acid, phenol), alone or in combination, on host-seeking behavior of stable flies has not yet been comprehensively investigated. In laboratory and greenhouse experiments, we tested the hypotheses that (1) CO<sub>2</sub> and CH<sub>4</sub> interactively attract stable flies, (2) CO<sub>2</sub> 'gates' attraction of stable flies to CH<sub>4</sub>, and (3) breath volatiles on their own, or in combination with both CO<sub>2</sub> and CH<sub>4</sub>, attract stable flies. In Y-tube olfactometer experiments, the blend of CH<sub>4</sub> (0.5%) and CO<sub>2</sub> (1%) in breathing air ('b-air') attracted significantly more female flies than CH<sub>4</sub>, or CO<sub>2</sub>, in b-air. The flies' responses to CH<sub>4</sub> were contingent upon their prior or concurrent exposure to CO<sub>2</sub>. In two-choice experiments in a large greenhouse compartment, significantly more flies landed on the host-look-alike barrel that disseminated a blend of CO<sub>2</sub> and CH<sub>4</sub> in b-air (CO<sub>2</sub>/CH<sub>4</sub>/b-air) than on the barrel disseminating either b-air or CO<sub>2</sub>. Moreover, significantly more flies landed on the barrel that disseminated synthetic breath volatiles (SBVs) than on the barrel disseminating b-air. The blend of CO<sub>2</sub>/CH<sub>4</sub>/b-air and SBVs elicited more fly landings on barrels than CO<sub>2</sub>/CH<sub>4</sub>/b-air but not than SBVs. SBVs, possibly combined with both CH<sub>4</sub> and CO<sub>2</sub>, could be developed as a lure to enhance trap captures of stable flies in livestock production facilities.</p>","PeriodicalId":15346,"journal":{"name":"Journal of Chemical Ecology","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141160718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-20DOI: 10.1007/s10886-024-01510-0
Tobias Frühbrodt, Baoguo Du, Horst Delb, Tim Burzlaff, Jürgen Kreuzwieser, Peter H W Biedermann
{"title":"Correction: Know When You Are Too Many: Density-Dependent Release of Pheromones During Host Colonisation by the European Spruce Bark Beetle, Ips Typographus (L.).","authors":"Tobias Frühbrodt, Baoguo Du, Horst Delb, Tim Burzlaff, Jürgen Kreuzwieser, Peter H W Biedermann","doi":"10.1007/s10886-024-01510-0","DOIUrl":"https://doi.org/10.1007/s10886-024-01510-0","url":null,"abstract":"","PeriodicalId":15346,"journal":{"name":"Journal of Chemical Ecology","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141065447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-13DOI: 10.1007/s10886-024-01500-2
Zhicai Luo, Yan Zhang, Panpan Zhang, Leyuan Liu, Jinxi Yuan, Shanchun Yan, Wei Liu, Guirong Wang
The Oriental fruit fly, Bactrocera dorsalis, is a significant pest that damages a variety of fruit crops. The effectiveness of chemical pesticides against such pests is limited, raising concerns about pesticide residues and resistance. Proteins naturally attract B. dorsalis and have led to the development of a management strategy known as protein bait attractant technology (BAT). Although the attraction of protein sources to B. dorsalis is well-documented, the biologically active components within these sources are not fully understood. This study employed analytical chemistry, behavioral tests, and electrophysiological techniques to investigate the behaviorally active components of beer yeast protein powder (BYPD), aiming to provide a basis for improving and developing protein baits. An olfactory trap assay confirmed the attractiveness of BYPD, and five components with high abundance were identified from its headspace volatiles using GC-MS. These components included ethanol, isoamyl alcohol, ethyl decanoate, benzaldehyde, and phenylethyl alcohol. Mixtures of these five components demonstrated significant attraction to B. dorsalis adults, with benzaldehyde identified as a potential key component. The attractiveness of benzaldehyde required a relatively large dose, and it was most attractive to adults that had been starved from dusk until the following morning. Attraction of adult flies to benzaldehyde appeared mainly mediated by inputs from olfactory receptors. While EAG data supports that ionotropic receptors could influence the detection of benzaldehyde in female adults, they did not affect female behavior towards benzaldehyde. These findings indicate that benzaldehyde is an important behaviorally active component in BYPD and offer insights for developing novel protein lures to control B. dorsalis in an environmentally friendly manner.
{"title":"Benzaldehyde acts as a behaviorally active component in brewer's yeast protein powder which attracts B. dorsalis through olfaction.","authors":"Zhicai Luo, Yan Zhang, Panpan Zhang, Leyuan Liu, Jinxi Yuan, Shanchun Yan, Wei Liu, Guirong Wang","doi":"10.1007/s10886-024-01500-2","DOIUrl":"https://doi.org/10.1007/s10886-024-01500-2","url":null,"abstract":"<p><p>The Oriental fruit fly, Bactrocera dorsalis, is a significant pest that damages a variety of fruit crops. The effectiveness of chemical pesticides against such pests is limited, raising concerns about pesticide residues and resistance. Proteins naturally attract B. dorsalis and have led to the development of a management strategy known as protein bait attractant technology (BAT). Although the attraction of protein sources to B. dorsalis is well-documented, the biologically active components within these sources are not fully understood. This study employed analytical chemistry, behavioral tests, and electrophysiological techniques to investigate the behaviorally active components of beer yeast protein powder (BYPD), aiming to provide a basis for improving and developing protein baits. An olfactory trap assay confirmed the attractiveness of BYPD, and five components with high abundance were identified from its headspace volatiles using GC-MS. These components included ethanol, isoamyl alcohol, ethyl decanoate, benzaldehyde, and phenylethyl alcohol. Mixtures of these five components demonstrated significant attraction to B. dorsalis adults, with benzaldehyde identified as a potential key component. The attractiveness of benzaldehyde required a relatively large dose, and it was most attractive to adults that had been starved from dusk until the following morning. Attraction of adult flies to benzaldehyde appeared mainly mediated by inputs from olfactory receptors. While EAG data supports that ionotropic receptors could influence the detection of benzaldehyde in female adults, they did not affect female behavior towards benzaldehyde. These findings indicate that benzaldehyde is an important behaviorally active component in BYPD and offer insights for developing novel protein lures to control B. dorsalis in an environmentally friendly manner.</p>","PeriodicalId":15346,"journal":{"name":"Journal of Chemical Ecology","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140916041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-10DOI: 10.1007/s10886-024-01509-7
Xiao-Jin Pei, Coby Schal, Yong-Liang Fan
Insect cuticular hydrocarbons (CHCs) serve as important waterproofing barriers and as signals and cues in chemical communication. Over the past 30 years, numerous studies on CHCs have been conducted in the German cockroach, Blattella germanica, leading to substantial progress in the field. However, there has not been a systematic review of CHC studies in this species in recent years. This review aims to provide a concise overview of the chemical composition, storage, transport, and physical properties of different CHCs in B. germanica. Additionally, we focus on the biosynthetic pathway and the genetic regulation of HC biosynthesis in this species. A considerable amount of biochemical evidence regarding the biosynthetic pathway of insect CHCs has been gathered from studies conducted in B. germanica. In recent years, there has also been an improved understanding of the molecular mechanisms that underlie CHC production in this insect. In this article, we summarize the biosynthesis of different classes of CHCs in B. germanica. Then, we review CHCs reaction to various environmental conditions and stressors and internal physiological states. Additionally, we review a body of work showing that in B. germanica, CHC profiles exhibit significant sexual dimorphism, specific CHCs act as essential precursors for female contact sex pheromone components, and we summarize the molecular regulatory mechanisms that underlie sexual dimorphism of CHC profiles. Finally, we highlight future directions and challenges in research on the biosynthesis and regulatory mechanisms of CHCs in B. germanica, and also identify potential applications of CHC studies in the pest control.
昆虫的角质层碳氢化合物(CHC)是重要的防水屏障,也是化学交流的信号和线索。在过去的 30 年中,对德国小蠊的 CHCs 进行了大量研究,在该领域取得了重大进展。然而,近年来还没有对该物种的 CHC 研究进行系统综述。本综述旨在简要概述德国蜚蠊体内不同 CHC 的化学成分、储存、运输和物理性质。此外,我们还将重点关注该物种中碳氢化合物生物合成的生物合成途径和遗传调控。有关昆虫 CHC 生物合成途径的大量生化证据都是通过对 B. germanica 的研究收集的。近年来,人们对这种昆虫产生 CHC 的分子机制也有了更深入的了解。在本文中,我们总结了 B. germanica 不同种类 CHC 的生物合成过程。然后,我们回顾了 CHCs 对各种环境条件和压力以及内部生理状态的反应。此外,我们还回顾了一系列工作,这些工作表明,在德国蝙蝠中,CHC谱系表现出显著的性二态性,特定的CHC是雌性接触性信息素成分的重要前体,我们还总结了CHC谱系性二态性的分子调控机制。最后,我们强调了研究德国蝙蝠 CHC 的生物合成和调控机制的未来方向和挑战,并指出了 CHC 研究在害虫控制中的潜在应用。
{"title":"Genetic Underpinnings of Cuticular Hydrocarbon Biosynthesis in the German Cockroach, Blattella germanica (L.): Progress and Perspectives.","authors":"Xiao-Jin Pei, Coby Schal, Yong-Liang Fan","doi":"10.1007/s10886-024-01509-7","DOIUrl":"https://doi.org/10.1007/s10886-024-01509-7","url":null,"abstract":"<p><p>Insect cuticular hydrocarbons (CHCs) serve as important waterproofing barriers and as signals and cues in chemical communication. Over the past 30 years, numerous studies on CHCs have been conducted in the German cockroach, Blattella germanica, leading to substantial progress in the field. However, there has not been a systematic review of CHC studies in this species in recent years. This review aims to provide a concise overview of the chemical composition, storage, transport, and physical properties of different CHCs in B. germanica. Additionally, we focus on the biosynthetic pathway and the genetic regulation of HC biosynthesis in this species. A considerable amount of biochemical evidence regarding the biosynthetic pathway of insect CHCs has been gathered from studies conducted in B. germanica. In recent years, there has also been an improved understanding of the molecular mechanisms that underlie CHC production in this insect. In this article, we summarize the biosynthesis of different classes of CHCs in B. germanica. Then, we review CHCs reaction to various environmental conditions and stressors and internal physiological states. Additionally, we review a body of work showing that in B. germanica, CHC profiles exhibit significant sexual dimorphism, specific CHCs act as essential precursors for female contact sex pheromone components, and we summarize the molecular regulatory mechanisms that underlie sexual dimorphism of CHC profiles. Finally, we highlight future directions and challenges in research on the biosynthesis and regulatory mechanisms of CHCs in B. germanica, and also identify potential applications of CHC studies in the pest control.</p>","PeriodicalId":15346,"journal":{"name":"Journal of Chemical Ecology","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140898406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-09DOI: 10.1007/s10886-024-01507-9
Rabiatul Addawiyah Shamshir, Suk-Ling Wee
Zeugodacus cucurbitae and Z. tau are two major fruit fly pests of cucurbitaceous plants in the tropical and subtropical regions. The former species has a broader host range and wider world distribution than the latter. With global climate change, Z. tau shows great potential for geographical expansion with several invasion records in recent years. Males of both species are attracted to cue lure (CL) (and raspberry ketone (RK), a deacetyl derivative of CL), a common male lure used in fruit fly population detection, monitoring and control programs. Males of both species are also known to respond to zingerone (ZN), which are produced by some rainforest orchids. Previous studies have shown that fruit fly-male lure interactions are unique to species and lure types, and significantly impact the success of a lure-based fruit fly control program. We seek to compare the attraction of Z. cucurbitae and Z. tau males to CL, RK and ZN via Probit behavioral assays. Our results showed that CL is more attractive to Z. cucurbitae and Z. tau males than RK, while ZN is a poor lure for both species. Attraction Z. tau to CL is slightly lower than Z. cucurbitae, but the former is at least 1.71 times less attractive to RK than the latter. Together with published information on species' sexual development, our current study indicates a lure-based control program via male annihilation technique for Z. tau will be more challenging than Z. cucurbitae and should incorporate other integrated pest management strategies for a desirable outcome.
Zeugodacus cucurbitae 和 Z. tau 是热带和亚热带地区葫芦科植物的两种主要果蝇害虫。与后者相比,前者的寄主范围更广,在全球的分布也更广泛。随着全球气候变化,Z. tau 显示出地理扩张的巨大潜力,近年来已有多次入侵记录。这两个物种的雄性都会被诱饵(CL)(以及覆盆子酮(RK),CL的一种脱乙酰衍生物)吸引,这是果蝇种群检测、监测和控制项目中常用的雄性诱饵。已知这两种果蝇的雄蝇也会对一些热带雨林兰花产生的辛辣酮(ZN)产生反应。以前的研究表明,果蝇与雄性引诱剂之间的相互作用因物种和引诱剂类型而异,并对基于引诱剂的果蝇控制项目的成功与否产生重大影响。我们试图通过 Probit 行为测定来比较葫芦科果蝇(Z. cucurbitae)和头蝇(Z. tau)雄蝇对 CL、RK 和 ZN 的吸引力。我们的结果表明,CL 比 RK 对葫芦蝇和 Z. tau 雄蝇更有吸引力,而 ZN 对这两种蝇的引诱效果较差。Z. tau对CL的吸引力略低于Z. cucurbitae,但前者对RK的吸引力至少是后者的1.71倍。结合已公布的有关物种性发育的信息,我们目前的研究表明,通过雄性歼灭技术对 Z. tau 进行引诱控制比对 Z. cucurbitae 进行引诱控制更具挑战性,应结合其他虫害综合防治策略,以取得理想的效果。
{"title":"Comparative Responses of Two Major Cucurbit Pests, Zeugodacus cucurbitae and Zeugodacus tau to Phenylbutanoid Male Lures.","authors":"Rabiatul Addawiyah Shamshir, Suk-Ling Wee","doi":"10.1007/s10886-024-01507-9","DOIUrl":"https://doi.org/10.1007/s10886-024-01507-9","url":null,"abstract":"<p><p>Zeugodacus cucurbitae and Z. tau are two major fruit fly pests of cucurbitaceous plants in the tropical and subtropical regions. The former species has a broader host range and wider world distribution than the latter. With global climate change, Z. tau shows great potential for geographical expansion with several invasion records in recent years. Males of both species are attracted to cue lure (CL) (and raspberry ketone (RK), a deacetyl derivative of CL), a common male lure used in fruit fly population detection, monitoring and control programs. Males of both species are also known to respond to zingerone (ZN), which are produced by some rainforest orchids. Previous studies have shown that fruit fly-male lure interactions are unique to species and lure types, and significantly impact the success of a lure-based fruit fly control program. We seek to compare the attraction of Z. cucurbitae and Z. tau males to CL, RK and ZN via Probit behavioral assays. Our results showed that CL is more attractive to Z. cucurbitae and Z. tau males than RK, while ZN is a poor lure for both species. Attraction Z. tau to CL is slightly lower than Z. cucurbitae, but the former is at least 1.71 times less attractive to RK than the latter. Together with published information on species' sexual development, our current study indicates a lure-based control program via male annihilation technique for Z. tau will be more challenging than Z. cucurbitae and should incorporate other integrated pest management strategies for a desirable outcome.</p>","PeriodicalId":15346,"journal":{"name":"Journal of Chemical Ecology","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140898312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-09DOI: 10.1007/s10886-024-01501-1
Bashiru Adams, Abdullahi Ahmed Yusuf, Baldwyn Torto, Fathiya Mbarak Khamis
The zoophytophagous mirid predator Nesidiocoris tenuis and the ectoparasitoid Stenomesius japonicus are important biological control agents for several agricultural pests including the invasive leafminer, Phthorimaea absoluta, a destructive pest of Solanaceous crops especially tomato in sub-Saharan Africa. However, little is known about how feeding by N. tenuis can influence the tritrophic interactions in the tomato plant. Here, we tested the hypothesis that N. tenuis phytophagy would influence the tritrophic olfactory interactions between the host plant tomato and pest, predator, and parasitoid. In olfactometer assays, P. absoluta females and N. tenuis adults were both attracted to constitutive volatiles released by the tomato plant. Whereas females of P. absoluta avoided volatiles released by N. tenuis-infested plants, S. japonicus females and N. tenuis adults were attracted to the induced volatiles. In coupled gas chromatography-electroantennographic detection (GC-EAD) recordings of intact and N. tenuis-infested plant volatiles, antennae of P. absoluta and S. japonicus females both detected eight components, whereas N. tenuis adults detected seven components which were identified by GC-mass spectrometry (GC-MS) as terpenes and green leaf volatiles (GLVs). Dose-response olfactometer bioassays revealed that the responses of P. absoluta, N. tenuis, and S. japonicus varied with the composition and concentration of blends and individual compounds tested from N tenuis-induced volatiles. Females of P. absoluta showed no preference for an eight-component blend formulated from the individual repellents including hexanal, (Z)-3-hexenyl butanoate, and δ-elemene identified in the volatiles. On the other hand, S. japonicus females were attracted to an eight-component blend including the attractants (E)-2-hexenal, (Z)-3-hexenol, methyl salicylate, β-phellandrene, and (E)-caryophyllene. Likewise, N. tenuis adults were attracted to a seven-component blend including the attractants β-phellandrene, δ-elemene, and (E)-caryophyllene identified in the volatiles. Our findings suggest that there is potential for the use of terpenes and GLVs to manage the insects in the tritrophic interaction.
食虫蜃天敌 Nesidiocoris tenuis 和外寄生虫 Stenomesius japonicus 是几种农业害虫的重要生物控制剂,包括入侵的潜叶蝇 Phthorimaea absoluta,它是撒哈拉以南非洲地区茄科作物尤其是番茄的一种毁灭性害虫。然而,人们对天牛的取食如何影响番茄植株的三营养交互作用知之甚少。在此,我们测试了天牛植食性会影响寄主植物番茄与害虫、捕食者和寄生虫之间三营养体嗅觉相互作用的假设。在嗅觉试验中,P. absoluta雌虫和N. tenuis成虫都会被番茄植株释放的组成挥发物所吸引。雌性 P. absoluta 会避开 N. tenuis 侵染植物释放的挥发性物质,而雌性 S. japonicus 和 N. tenuis 成虫则会被诱导的挥发性物质吸引。在耦合气相色谱-电触角检测(GC-EAD)记录完整植物和受天牛侵染植物的挥发物时,绝对雌虫和日本绣线菊雌虫的触角都能检测到八种成分,而天牛成虫则能检测到七种成分,这些成分经气相色谱-质谱联用仪(GC-MS)鉴定为萜烯类和绿叶挥发物(GLVs)。剂量-反应嗅觉仪生物测定显示,P. absoluta、N. tenuis 和 S. japonicus 的反应随 N tenuis 诱导挥发物的混合物和单个测试化合物的成分和浓度而变化。雌性 P. absoluta 对由单个驱避剂(包括挥发物中发现的己醛、(Z)-3-己烯基丁酸酯和δ-榄香烯)配制而成的八组分混合物没有表现出偏好。另一方面,日本绢毛虫雌虫会被八种混合成分吸引,其中包括吸引剂 (E)-2- 己烯醛、(Z)-3-己烯醇、水杨酸甲酯、β-黄柏烯和 (E)- 卡里叶烯。同样,N. tenuis 成虫也会被七种成分的混合物吸引,其中包括在挥发性物质中发现的引诱剂 β-黄柏烯、δ-榄香烯和 (E)- 卡里叶烯。我们的研究结果表明,利用萜烯和 GLVs 在三营养交互作用中管理昆虫是有潜力的。
{"title":"Tritrophic Interactions Mediated by Zoophytophagous Predator-Induced Host Plant Volatiles.","authors":"Bashiru Adams, Abdullahi Ahmed Yusuf, Baldwyn Torto, Fathiya Mbarak Khamis","doi":"10.1007/s10886-024-01501-1","DOIUrl":"https://doi.org/10.1007/s10886-024-01501-1","url":null,"abstract":"<p><p>The zoophytophagous mirid predator Nesidiocoris tenuis and the ectoparasitoid Stenomesius japonicus are important biological control agents for several agricultural pests including the invasive leafminer, Phthorimaea absoluta, a destructive pest of Solanaceous crops especially tomato in sub-Saharan Africa. However, little is known about how feeding by N. tenuis can influence the tritrophic interactions in the tomato plant. Here, we tested the hypothesis that N. tenuis phytophagy would influence the tritrophic olfactory interactions between the host plant tomato and pest, predator, and parasitoid. In olfactometer assays, P. absoluta females and N. tenuis adults were both attracted to constitutive volatiles released by the tomato plant. Whereas females of P. absoluta avoided volatiles released by N. tenuis-infested plants, S. japonicus females and N. tenuis adults were attracted to the induced volatiles. In coupled gas chromatography-electroantennographic detection (GC-EAD) recordings of intact and N. tenuis-infested plant volatiles, antennae of P. absoluta and S. japonicus females both detected eight components, whereas N. tenuis adults detected seven components which were identified by GC-mass spectrometry (GC-MS) as terpenes and green leaf volatiles (GLVs). Dose-response olfactometer bioassays revealed that the responses of P. absoluta, N. tenuis, and S. japonicus varied with the composition and concentration of blends and individual compounds tested from N tenuis-induced volatiles. Females of P. absoluta showed no preference for an eight-component blend formulated from the individual repellents including hexanal, (Z)-3-hexenyl butanoate, and δ-elemene identified in the volatiles. On the other hand, S. japonicus females were attracted to an eight-component blend including the attractants (E)-2-hexenal, (Z)-3-hexenol, methyl salicylate, β-phellandrene, and (E)-caryophyllene. Likewise, N. tenuis adults were attracted to a seven-component blend including the attractants β-phellandrene, δ-elemene, and (E)-caryophyllene identified in the volatiles. Our findings suggest that there is potential for the use of terpenes and GLVs to manage the insects in the tritrophic interaction.</p>","PeriodicalId":15346,"journal":{"name":"Journal of Chemical Ecology","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140898408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-01DOI: 10.1007/s10886-024-01494-x
Irene Castellan, Claire Duménil, Guillermo Rehermann, Daniela Eisenstecken, Flavia Bianchi, Peter Robatscher, Urban Spitaler, Riccardo Favaro, Silvia Schmidt, Paul G. Becher, Sergio Angeli
Chemical control of Drosophila suzukii (Diptera: Drosophilidae) based on the use of insecticides is particularly challenging as the insect attacks ripening fruits shortly before harvest. An alternative strategy may rely on the use of yeasts as phagostimulants and baits, applied on canopy as attract-and-kill formulations. The aim of this research was to identify the most attractive among six yeast species for D. suzukii: Saccharomyces cerevisiae, Hanseniaspora uvarum, Clavispora santaluciae, Saccharomycopsis vini, Issatchenkia terricola, and Metschnikowia pulcherrima. The volatile profile of C. santaluciae was described for the first time. Behavioural experiments identified H. uvarum and S. vini as the most attractive yeasts. The characterization of yeast headspace volatiles using direct headspace (DHS) and solid-phase microextraction (SPME) revealed several strain-specific compounds. With DHS injection, 19 volatiles were characterised, while SPME revealed 71 compounds constituting the yeast headspace. Both analyses revealed terpenoids including β-ocimene, citronellol, (Z)-geraniol (nerol), and geranial as distinct constituents of S. vini. H. uvarum and S. vini were further investigated using closed-loop stripping analysis (CSLA) and electroantennography. Out of 14 compounds quantified by CSLA, ethyl acetate, isoamyl acetate, β-myrcene, benzaldehyde and linalool were detected by D. suzukii antennae and might generate the strong attractiveness of S. vini and H. uvarum. Our results highlight a strong attraction of D. suzukii to various yeasts associated with both the flies and their habitat and demonstrate how different sampling methods can impact the results of volatile compound characterization. It remains to be demonstrated whether the distinct attraction is based on special adaptations to certain yeasts and to what extent the metabolites causing attraction are interchangeable.
使用杀虫剂对铃木果蝇(双翅目:果蝇科)进行化学防治尤其具有挑战性,因为这种昆虫会在收获前不久攻击成熟的果实。另一种策略可能是使用酵母作为诱食剂和诱饵,在树冠上施用诱杀制剂。这项研究的目的是从六种酵母中找出对铃虫最有吸引力的一种:Saccharomyces cerevisiae、Hanseniaspora uvarum、Clavispora santaluciae、Saccharomycopsis vini、Issatchenkia terricola 和 Metschnikowia pulcherrima。首次描述了 C. santaluciae 的挥发性特征。行为实验确定 H. uvarum 和 S. vini 是最有吸引力的酵母菌。利用直接顶空(DHS)和固相微萃取(SPME)对酵母顶空挥发物进行表征,发现了几种菌株特有的化合物。通过 DHS 注入,对 19 种挥发物进行了表征,而 SPME 则揭示了构成酵母顶空的 71 种化合物。这两项分析都揭示了萜类化合物,包括 β-柠檬烯、香茅醇、(Z)-橙花醇(橙花醇)和香叶醛,它们是维尼酵母的独特成分。使用闭环剥离分析法(CSLA)和电触觉分析法对 H. uvarum 和 S. vini 进行了进一步研究。在 CSLA 定量分析的 14 种化合物中,铃兰触角检测到了乙酸乙酯、乙酸异戊酯、β-月桂烯、苯甲醛和芳樟醇,它们可能会产生 S. vini 和 H. uvarum 的强烈吸引力。我们的研究结果突显了 D. suzukii 对与苍蝇及其栖息地相关的各种酵母菌的强烈吸引力,并证明了不同的取样方法会如何影响挥发性化合物表征的结果。这种独特的吸引力是否基于对某些酵母菌的特殊适应性,以及在多大程度上导致吸引力的代谢物是可以互换的,这些都有待证明。
{"title":"Chemical and Electrophysiological Characterisation of Headspace Volatiles from Yeasts Attractive to Drosophila suzukii","authors":"Irene Castellan, Claire Duménil, Guillermo Rehermann, Daniela Eisenstecken, Flavia Bianchi, Peter Robatscher, Urban Spitaler, Riccardo Favaro, Silvia Schmidt, Paul G. Becher, Sergio Angeli","doi":"10.1007/s10886-024-01494-x","DOIUrl":"https://doi.org/10.1007/s10886-024-01494-x","url":null,"abstract":"<p>Chemical control of <i>Drosophila suzukii</i> (Diptera: Drosophilidae) based on the use of insecticides is particularly challenging as the insect attacks ripening fruits shortly before harvest. An alternative strategy may rely on the use of yeasts as phagostimulants and baits, applied on canopy as attract-and-kill formulations. The aim of this research was to identify the most attractive among six yeast species for <i>D. suzukii</i>: <i>Saccharomyces cerevisiae</i>, <i>Hanseniaspora uvarum</i>, <i>Clavispora santaluciae</i>, <i>Saccharomycopsis vini</i>, <i>Issatchenkia terricola</i>, and <i>Metschnikowia pulcherrima</i>. The volatile profile of <i>C</i>. <i>santaluciae</i> was described for the first time. Behavioural experiments identified <i>H. uvarum</i> and <i>S. vini</i> as the most attractive yeasts. The characterization of yeast headspace volatiles using direct headspace (DHS) and solid-phase microextraction (SPME) revealed several strain-specific compounds. With DHS injection, 19 volatiles were characterised, while SPME revealed 71 compounds constituting the yeast headspace. Both analyses revealed terpenoids including β-ocimene, citronellol, (<i>Z</i>)-geraniol (nerol), and geranial as distinct constituents of <i>S. vini</i>. <i>H. uvarum</i> and <i>S. vini</i> were further investigated using closed-loop stripping analysis (CSLA) and electroantennography. Out of 14 compounds quantified by CSLA, ethyl acetate, isoamyl acetate, β-myrcene, benzaldehyde and linalool were detected by <i>D. suzukii</i> antennae and might generate the strong attractiveness of <i>S. vini</i> and <i>H. uvarum.</i> Our results highlight a strong attraction of <i>D. suzukii</i> to various yeasts associated with both the flies and their habitat and demonstrate how different sampling methods can impact the results of volatile compound characterization. It remains to be demonstrated whether the distinct attraction is based on special adaptations to certain yeasts and to what extent the metabolites causing attraction are interchangeable.</p>","PeriodicalId":15346,"journal":{"name":"Journal of Chemical Ecology","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140835468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-22DOI: 10.1007/s10886-024-01499-6
K. Tan, Ritsuo Nishida
{"title":"A review on natural phenylbutanoid attractants: Occurrence, distribution, and role in nature, especially in relation to Dacini fruit fly behavior and pollination.","authors":"K. Tan, Ritsuo Nishida","doi":"10.1007/s10886-024-01499-6","DOIUrl":"https://doi.org/10.1007/s10886-024-01499-6","url":null,"abstract":"","PeriodicalId":15346,"journal":{"name":"Journal of Chemical Ecology","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140674647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-22DOI: 10.1007/s10886-024-01493-y
Emily M. Russavage, Jeremy A. Hewlett, John M Grunseich, Adrianna Szczepaniec, William L. Rooney, A. Helms, M. Eubanks
{"title":"Aphid-Induced Volatiles and Subsequent Attraction of Natural Enemies Varies among Sorghum Cultivars.","authors":"Emily M. Russavage, Jeremy A. Hewlett, John M Grunseich, Adrianna Szczepaniec, William L. Rooney, A. Helms, M. Eubanks","doi":"10.1007/s10886-024-01493-y","DOIUrl":"https://doi.org/10.1007/s10886-024-01493-y","url":null,"abstract":"","PeriodicalId":15346,"journal":{"name":"Journal of Chemical Ecology","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140673131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}