Journal of chromatography. B, Biomedical sciences and applications最新文献

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Fast detection of phosphorylation of human pepsinogen A, human pepsinogen C and swine pepsinogen using a combination of reversed-phase high-performance liquid chromatography and capillary zone electrophoresis for peptide mapping. 利用反相高效液相色谱法和毛细管区带电泳法快速检测人胃蛋白酶原A、人胃蛋白酶原C和猪胃蛋白酶原的磷酸化。

Journal of chromatography. B, Biomedical sciences and applications

Pub Date : 1997-01-24

R Hynek, V Kasicka, Z Kucerová, J Kás

A combination of reversed-phase high-performance liquid chromatography (RP-HPLC) and capillary zone electrophoresis (CZE) was used for characterization of alpha-chymotryptic digests of human pepsinogen A, human pepsinogen C (both isolated from stomach mucosa of patients suffering from gastric cancer), swine pepsinogen and their dephosphorylated forms. Combining RP-HPLC and CZE for peptide mapping allowed to detect phosphorylations in molecules of the above mentioned gastric zymogens. We have found one phosphate group in the molecule of human pepsinogen A and two phosphate groups in the molecule of human pepsinogen C. The investigated sample was obtained from stomach mucosa of a patient suffering from gastric cancer. An increased number of phosphate groups in molecules of human pepsinogen seems to be associated with gastric cancer. The developed method represent a suitable tool for studying relationships between specific phosphorylations of proteins and cancerogenesis or potentially could serve for early diagnosis of gastric cancer.

采用反相高效液相色谱(RP-HPLC)和毛细管区带电泳(CZE)相结合的方法，对人胃蛋白酶原A、人胃蛋白酶原C(均分离自胃癌患者胃粘膜)、猪胃蛋白酶原及其去磷酸化形式进行了α -胰酶消化的表征。结合RP-HPLC和CZE进行肽定位，可以检测上述胃酶原分子的磷酸化。我们在人胃蛋白酶原A分子中发现一个磷酸基团，在人胃蛋白酶原c分子中发现两个磷酸基团。所研究的样品取自胃癌患者的胃粘膜。人胃蛋白酶原分子中磷酸基团数量的增加似乎与胃癌有关。该方法为研究特定蛋白磷酸化与癌变之间的关系提供了一种合适的工具，或可能用于胃癌的早期诊断。

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