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Antimicrobial susceptibility profile and detection of extended spectrum β-lactamase resistance genes in pseudomonas aeruginosa isolated from post-operative wounds 术后创面铜绿假单胞菌抗药谱及β-内酰胺酶耐药基因的检测
Pub Date : 2023-03-20 DOI: 10.18488/99.v10i1.3326
M. Ja’afaru, M. Bashir, F. F. Tizhe, N. A. Kachalla
The spread of Pseudomonas aeruginosa is difficult to control with disinfectants and antibiotics due to the resistance found in P. aeruginosa isolates. The study investigated the susceptibility profile and detect extended spectrum β-lactamase (ESBL) resistance genes in the Pseudomonas aeruginosa isolated from post-operative wound infection. Wound swabs were cultured on MacConkey and sub-cultured on Cetrimide agar. The isolates were identified base on their macroscopic and microscopic characteristics as well as 16SrRNA. Disc diffusion technique was used to check the susceptibility profile of the isolates and the extended spectrum beta-lactam (ESBL) producers were determined by growing the isolates on Mueller Hinton agar plate in the presence of amoxyclav. 40 positive isolates were recovered in the study with 21 isolates been multi-drug resistance (MDR). Before plasmid curing, Streptomycin, ceftazidime, Augmentin, and Gentamicin has the highest resistance rate of 80% (n=32), 60% (n=24), 60% (n=24) and 60% (n=24) respectively. However, after plasmid curing, the result showed reduction in resistance rates. Streptomycin and ceftazidime showed high reduction in resistance from 80% to 25% and from 60 to 12.7% respectively indicating that resistance was plasmid-mediated. Pseudomonas aeruginosa F23 and Pseudomonas aeruginosa S15 isolates shown to possess both the plcH and bla PER genes with bands amplicons size of 307bp and 520bp respectively. Antibiotic susceptibility pattern after plasmid curing showed that 45% of the isolates produces beta-lactamase enzymes i.e., 18 out of 40 isolates were inactive to all beta-lactam group of antibiotics.
铜绿假单胞菌的传播很难用消毒剂和抗生素控制,因为铜绿假单胞菌分离株具有耐药性。研究了术后伤口感染铜绿假单胞菌的药敏特征,并检测了其延伸谱β-内酰胺酶(ESBL)耐药基因。伤口拭子在MacConkey培养基上培养,在Cetrimide琼脂培养基上继代培养。根据其宏观和微观特征以及16SrRNA进行鉴定。采用圆盘扩散技术检测菌株的药敏谱,并在氨氧克虫存在的条件下,在Mueller Hinton琼脂平板上培养菌株,确定其β -内酰胺(ESBL)产生菌。共检出阳性菌株40株,其中耐多药菌株21株。质粒固化前,链霉素、头孢他啶、奥格门汀、庆大霉素的耐药率最高,分别为80% (n=32)、60% (n=24)、60% (n=24)、60% (n=24)。然而,质粒固化后,结果显示抗性降低。链霉素和头孢他啶的耐药性分别从80%降低到25%和从60%降低到12.7%,表明耐药性是质粒介导的。铜绿假单胞菌F23和铜绿假单胞菌S15分离株均具有plcH和bla PER基因,条带扩增大小分别为307bp和520bp。质粒固化后的药敏模式显示,45%的菌株产生β -内酰胺酶,即40株菌株中有18株对所有β -内酰胺类抗生素均无活性。
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引用次数: 0
苦豆子药渣中多糖除蛋白质工艺优化研究 苦豆子药渣中多糖除蛋白质工艺优化研究
Pub Date : 2022-12-15 DOI: 10.19891/j.issn1673-9388.(2022)06-0421-05
高晓娟 | 冯莉 | 苏慧敏 | 王汉卿
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引用次数: 0
Key Breeding Containers of Dengue Vectors in Outbreak Localities in Federal Territory of Kuala Lumpur 吉隆坡联邦直辖区爆发地点登革热媒介的主要繁殖容器
Pub Date : 2021-01-01 DOI: 10.18488/JOURNAL.99.2021.81.1.8
F. A. Rahim, M. A. F. Mahmud, Mohd Farihan Md Yatim
Dengue is a mosquito-borne disease that rapidly spreads throughout tropical and subtropical regions. An estimated 2.5 billion people live in dengue-endemic countries and millions of cases occurring each year. Identifying key breeding containers are important to prevent dengue epidemics. This study aims to identify key breeding containers and to evaluate the risks of dengue transmission in the dengue outbreak localities in Kepong District, Kuala Lumpur. Entomological surveillance was done between January 2016 to December 2017 in 38 different outbreak localities. Both indoors and outdoors water-holding containers were inspected for the presence of mosquito larvae. All collected larvae have identified the species, types of breeding containers were recorded, and three larval indices, House Index (HI), Container Index (CI), and Breteau Index (BI), were calculated. Of the 2,067 containers inspected, 227 (10.98%) containers were positive for Aedes larvae. The four main breeding container types in this study were plastic containers (31.72%), water drums (22.47%), drains (18.06%), and flowerpots (14.98%). The HI, CI, and BI varied from 0 to 52.94, from 1.72 to 32.76, and from 2.33 to 75.00, respectively. Key breeding containers of Aedes larvae found in this study provide guidance for health service providers to educate residents to eliminate the common indoors and outdoors Aedes mosquito breeding containers in the study area.
登革热是一种蚊媒疾病,在热带和亚热带地区迅速传播。估计有25亿人生活在登革热流行国家,每年发生数百万例病例。确定关键的繁殖容器对预防登革热流行很重要。本研究旨在确定吉隆坡吉榜区登革热暴发地点的关键繁殖容器并评估登革热传播风险。2016年1月至2017年12月,在38个不同的疫情发生地进行了昆虫学监测。对室内和室外装水容器进行了检查,看是否有蚊子幼虫。采集的所有幼虫均进行种类鉴定、饲养容器类型记录,并计算幼虫的House指数、Container指数和Breteau指数。在2067个集装箱中,检出伊蚊幼虫227个(10.98%)。主要养殖容器类型为塑料容器(31.72%)、水桶(22.47%)、排水沟(18.06%)和花盆(14.98%)。HI、CI、BI分别为0 ~ 52.94、1.72 ~ 32.76、2.33 ~ 75.00。本研究发现的主要伊蚊幼虫孳生容器可指导卫生服务提供者教育居民消除研究区常见的室内和室外伊蚊孳生容器。
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引用次数: 1
A Systematic Analysis of Laboratory-Guided Diagnosis and Management of Covid-19: Challenges and Recommendations Covid-19实验室指导诊断和管理的系统分析:挑战和建议
Pub Date : 2020-01-01 DOI: 10.18488/journal.99.2020.71.14.32
Whelan Shane, Keaney Daniel, Lucey Brigid, F. Karen
We conducted a comprehensive analysis of patient demographics and laboratory tests encompassing Real Time PCR (RT-qPCR) and serology for SARS-CoV-2 in addition to blood components and clinical blood markers for COVID-19 disease. All relevant literature was included up to 15 July 2020 and multiple studies were analysed in tandem to correlate findings. For RT-qPCR, nasopharyngeal swabs are the most suitable samples based on detection rates by sample, but may require repeat testing. One-week post-symptom onset, serological testing is a more stable marker. Antibody titers have been linked to disease severity. Several clinical blood components and markers have been reported to be prognostically useful; however, care is needed when making interpretation owing to the association between raised levels of these being found for the co-morbidities that predispose to worse prognosis in COVID-19. Challenges in the current study when finding the information presented in this paper suggest the need for a quality assured database that outlines the complete set of results, anonymised patient data for each entry and a set of internationally-agreed guidelines for complete laboratory testing, documentation and open-access reporting. We suggest that such information would be useful to help with patient diagnosis and management, worldwide. 8-14, addition a positive RT-qPCR test for SARS-CoV-2 from an upper respiratory tract Antibody levels using specificity assays 99.1% antibodies), 98.6% and The seroconversion rate for total antibody 93.1% (161/173), 82.7% (143/173) for IgM, and 64.7% for IgG. remained seronegative for total antibody, to unavailability of serum for testing the later stage (<13 days PSO) of their time for seroconversion day (total antibodies), day (IgM) and (IgG)
我们对患者人口统计学和实验室检测进行了全面分析,包括实时荧光定量PCR (RT-qPCR)和SARS-CoV-2的血清学检测,以及COVID-19疾病的血液成分和临床血液标志物。纳入截至2020年7月15日的所有相关文献,并对多项研究进行串联分析,以确定相关发现。对于RT-qPCR,基于各样本的检出率,鼻咽拭子是最合适的样本,但可能需要重复检测。症状出现后一周,血清学检测是更稳定的标志物。抗体滴度与疾病严重程度有关。据报道,一些临床血液成分和标记物对预后有帮助;然而,在进行解释时需要谨慎,因为在COVID-19中发现这些水平升高与易导致预后恶化的合并症之间存在关联。当前研究在寻找本文中提供的信息时面临的挑战表明,需要一个质量有保证的数据库,该数据库概述了完整的结果集,每个条目的匿名患者数据以及一套国际商定的完整实验室测试、文档和开放获取报告的指南。我们认为这些信息将有助于世界范围内的患者诊断和管理。8-14,另外对上呼吸道SARS-CoV-2进行RT-qPCR阳性检测,抗体水平特异性测定为99.1%,抗体特异性测定为98.6%,总抗体血清转化率为93.1% (161/173),IgM血清转化率为82.7% (143/173),IgG血清转化率为64.7%。血清总抗体仍呈阴性,无法获得血清用于检测后期(<13天PSO)的血清转化日(总抗体)、日(IgM)和(IgG)。
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引用次数: 1
Vital Statistics for Leading Countries in COVID-19 Cases 主要国家COVID-19病例的生命统计数据
Pub Date : 2020-01-01 DOI: 10.18488/journal.99.2020.71.6.13
Z. Singh
Coronavirus disease (COVID-19) is an infectious disease caused by a newly discovered coronavirus and the overall number of the COVID-19 cases is increasing worldwide. As on 24th March, 2020, China, Italy and United States were found to be the countries with maximum number of reported cases. China seemed to manage this outbreak with only 3,281 deaths for 81,218 cases with a death rate of 0.040 and a recovery record of 73,650 patients. The present review emphasizes the comparative patterns with which this pandemic spread in the above mentioned three countries. Paper also discusses total cases, daily recorded cases and total deaths of patients with daily death records. Conclusively, COVID-19 is affecting the population globally at a very rapid rate and should be dealt with maximum efforts by all the countries. Contribution/Originality: This study documents the comparative patterns with which this pandemic spread among the leading countries including China, Italy and United States of America. The present paper also discusses total cases, daily recorded cases and total deaths of patients with daily death records.
冠状病毒病(COVID-19)是由一种新发现的冠状病毒引起的传染病,全球COVID-19病例总数正在增加。截至2020年3月24日,中国、意大利和美国是报告病例数最多的国家。中国在81218例新冠肺炎病例中只有3281人死亡,死亡率为0.040,康复记录为73650人,似乎得到了控制。本审查报告强调这一流行病在上述三个国家传播的比较模式。文章还讨论了死亡日记录患者的总病例数、日记录病例数和死亡日记录患者的总死亡人数。最后,COVID-19正在以非常快的速度影响全球人口,各国应尽最大努力应对。贡献/独创性:本研究记录了这一流行病在包括中国、意大利和美利坚合众国在内的主要国家传播的比较模式。本文还讨论了死亡日记录患者的总病例数、日记录病例数和总死亡数。
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引用次数: 0
Chronic Hepatitis B Virus Infection in Four (4) Asymptomatic Prospective Employees: A Short Correspondence 四(4)名无症状潜在员工的慢性乙型肝炎病毒感染:简短通信
Pub Date : 2020-01-01 DOI: 10.18488/journal.99.2020.71.1.5
Ato Kwamena Tetteh, Charles Baffe, Prince Bram, S. Arthur, Godsway Aglago, E. Agyarko
Aim: This study aimed at compiling all hepatitis B results for prospective employees screened at the Cape Coast Metropolitan Hospital. This information is relevant in identifying those who are already infected, as well as susceptible individuals, to promote the establishment of prevention and control measures within work environments. Method: This study extracted data from laboratory records into Microsoft Excel and used for the analysis. Results: In this report, 13.3% (4/30) of prospective employees were found to be sero-positive for hepatitis B surface antigen (HBsAg). The remaining were sero-negative (susceptible), and required vaccination. All of the four (4) found to be sero-positive were chronically infected [both hepatitis B envelope antibody (HBeAb) and core antibody (HBcAb) - positive], that is, capable of transmitting to susceptible individuals if the proper infection prevention practices are not well implemented in their homes and work places. Only males were found to be positive, while all females were negative. Conclusion: Continuous education, screening, management of infected individuals and vaccination of the susceptible, will go a long way to curbing the spread of hepatitis B infection. Contribution/Originality: This study contributes to existing information, and draws attention to occupational support in the management of hepatitis B infection.
目的:本研究旨在汇编所有在海岸角大都会医院筛选的潜在员工的乙型肝炎结果。这一信息有助于查明已感染者和易感人群,促进在工作环境中制定预防和控制措施。方法:本研究从实验室记录中提取数据,在Microsoft Excel中进行分析。结果:在本报告中,13.3%(4/30)的潜在员工被发现乙型肝炎表面抗原(HBsAg)血清阳性。其余为血清阴性(易感),需要接种疫苗。所有四(4)名血清阳性的人都是慢性感染[乙型肝炎包膜抗体(HBeAb)和核心抗体(HBcAb)阳性],也就是说,如果在家庭和工作场所没有很好地实施适当的感染预防措施,就能够传播给易感人群。只有男性呈阳性,而所有女性都呈阴性。结论:持续的教育、筛查、管理感染者和易感人群的疫苗接种,将对遏制乙型肝炎感染的传播有很大的帮助。贡献/独创性:本研究对现有信息有贡献,并引起对乙型肝炎感染管理中的职业支持的关注。
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引用次数: 0
Occurrence of Diabetes among Rural Women 农村妇女糖尿病的发生情况
Pub Date : 2020-01-01 DOI: 10.18488/journal.99.2020.72.33.38
J. Randhawa, Z. Singh, Sonia
Diabetes has already been described as an epidemic, and health care system around the world is bearing its pressure. Globally, it has to be dealt with huge care. In the present study, 1001 rural women (Age 21-90 years; mean age: 54 ± 3.2 years) from the northern region of Punjab were analyzed for the serum glucose levels, to estimations its occurrence/prevalence. The subjects were divided into different age groups. The study revealed a high percentage (46.2%) of subjects with serum glucose levels higher than the normal range of 70-110 mg/dl of blood. Age groups of 21-30 and 81-90 years showed a less prevalence of diabetes, whereas age groups between 31-40 and 41-50 years is most prone showing higher percentages (average 12.9 and 18.5%, respectively). The study is an indicative of a major crisis in the coming times. The data suggests that awareness drives and remedial measures should be enforced with immediate effect. Contribution/Originality: This study contributes to the existing literature related to prevalence of diabetes in India. This study is one of very few studies which have investigated the prevalence and awareness of diabetes among women of rural areas of Punjab. The paper's primary contribution is finding that rural women in age groups between 31-50 are most prone to diabetes.
糖尿病已经被描述为一种流行病,世界各地的卫生保健系统正承受着它的压力。在全球范围内,必须非常小心地处理它。在本研究中,1001名农村妇女(年龄21-90岁;对来自旁遮普北部地区的平均年龄:54±3.2岁)的患者进行血清葡萄糖水平分析,以估计其发生/流行情况。研究对象被分为不同的年龄组。研究显示,高比例(46.2%)的受试者血清葡萄糖水平高于70-110 mg/dl的正常范围。21-30岁和81-90岁年龄组的糖尿病患病率较低,而31-40岁和41-50岁年龄组的糖尿病患病率最高(平均分别为12.9%和18.5%)。这项研究预示着未来的一场重大危机。数据表明,提高认识和采取补救措施应立即生效。贡献/独创性:本研究对印度糖尿病患病率相关的现有文献做出了贡献。这项研究是调查旁遮普邦农村地区妇女糖尿病患病率和意识的极少数研究之一。这篇论文的主要贡献是发现31-50岁年龄段的农村妇女最容易患糖尿病。
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引用次数: 1
Self-Esteem, Optimism and Burden of Care among Relatives of Cardiovascular Disease Patients in Selected Tertiary Hospitals in Southwest, Nigeria 尼日利亚西南部部分三级医院心血管病患者亲属的自尊、乐观与护理负担
Pub Date : 2019-07-18 DOI: 10.18488/journal.99.2019.62.23.33
S. Babatunde, Taofik Olatunji Bankole, R. Yinusa
Article History Received: 12 March 2019 Revised: 15 April 2019 Accepted: 21 May 2019 Published: 18 July 2019
收稿日期:2019年3月12日修稿日期:2019年4月15日接收日期:2019年5月21日发布日期:2019年7月18日
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引用次数: 1
Determinant Factors and Spatial Distribution of Visceral Leishmaniasis in North Gondar in Selected Hospitals 贡达尔北部部分医院内脏利什曼病的决定因素及空间分布
Pub Date : 2019-01-01 DOI: 10.18488/journal.99.2019.62.81.89
Berhanehiwot Melesse, Salie Ayalew, Mandefro Abere
Article History Received: 18 July 2019 Revised: 21 August 2019 Accepted: 24 September 2019 Published: 12 November 2019
收稿日期:2019年7月18日修订日期:2019年8月21日接收日期:2019年9月24日发布日期:2019年11月12日
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引用次数: 1
Staining Effect of Methanolic Extract of Hibiscus Sabdariffa Calyx on Thin Peripheral Blood Smear 芙蓉花萼甲醇提取物对外周血涂片的染色作用
Pub Date : 2019-01-01 DOI: 10.18488/journal.99.2019.61.1.6
E. Etim, Ezekiel Friyanda Chagwa, E. Obeagu, Akpan Akaninyene Udokang
Article History Received: 9 October 2018 Revised: 14 November 2018 Accepted: 19 December 2018 Published: 4 February 2019
收稿日期:2018年10月9日修稿日期:2018年11月14日收稿日期:2018年12月19日发布日期:2019年2月4日
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引用次数: 0
期刊
疾病监测与控制
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