{"title":"Blood, sweat, and light (no tears!): from treatment of vascular disorders to cancer detection","authors":"J. Barton","doi":"10.1117/12.2525154","DOIUrl":"https://doi.org/10.1117/12.2525154","url":null,"abstract":"","PeriodicalId":157928,"journal":{"name":"Optical Interactions with Tissue and Cells XXX","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117266762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Understanding free-electron mediated effects of tightly focused femtosecond pulse series is essential for minimizing photodamage in nonlinear microscopy and opens new avenues for nanosurgery and intentional modifications of biomolecules. We tracked different stages of the photomodification kinetics (hyperfluorescence, plasma luminescence, bubble formation) by time-lapse 2-photon microscopy, fluorescence lifetime measurements, and bubble interferometry with nanometer resolution. Monitoring of bubble growth during pulse series enabled us to quantify chemical reaction rates leading to gas formation via molecular disintegration. Novel ways of data evaluation were used to create a comprehensive picture of the photomodification kinetics in the (irradiance/irradiation dose) parameter space.
{"title":"Free electron mediated effects of femtosecond pulse series in the (irradiance/irradiation dose) parameter space (Conference Presentation)","authors":"N. Linz, Xiao-Xuan Liang, S. Freidank, A. Vogel","doi":"10.1117/12.2525153","DOIUrl":"https://doi.org/10.1117/12.2525153","url":null,"abstract":"Understanding free-electron mediated effects of tightly focused femtosecond pulse series is essential for minimizing photodamage in nonlinear microscopy and opens new avenues for nanosurgery and intentional modifications of biomolecules. We tracked different stages of the photomodification kinetics (hyperfluorescence, plasma luminescence, bubble formation) by time-lapse 2-photon microscopy, fluorescence lifetime measurements, and bubble interferometry with nanometer resolution. Monitoring of bubble growth during pulse series enabled us to quantify chemical reaction rates leading to gas formation via molecular disintegration. Novel ways of data evaluation were used to create a comprehensive picture of the photomodification kinetics in the (irradiance/irradiation dose) parameter space.","PeriodicalId":157928,"journal":{"name":"Optical Interactions with Tissue and Cells XXX","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125665458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"30 years of Laser-Tissue Interaction and beyond","authors":"E. Jansen","doi":"10.1117/12.2525152","DOIUrl":"https://doi.org/10.1117/12.2525152","url":null,"abstract":"","PeriodicalId":157928,"journal":{"name":"Optical Interactions with Tissue and Cells XXX","volume":"70 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121513151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Polarized light microscopy and polarimetry has been used to assess changes in cervical structure by targeting its collagen. 75% of the human cervix is in fact composed of highly arranged collagen which is birefringent. Recently we have used Mueller Matrix polarimetry to image the human cervix in-vivo to determine loss of collagen arrangement associated with later stages of pregnancy. In an effort to improve our system capability and better determine the provenance of the polarized signal we have developed a Polarized Light Monte Carlo model capable of characterizing polarized light interaction with a birefringent, scattering, and absorbing medium such as the cervix. We have utilized this model to investigate the effect of cervical collagen arrangement typical of late stage in pregnancy on polarized light. In this talk we will illustrate the model framework, its validations, and provide several test cases in model cervices.
{"title":"Polarized light Monte Carlo simulation of cervical collagen ultrastructure (Conference Presentation)","authors":"J. Chue-Sang, J. Ramella-Roman","doi":"10.1117/12.2508611","DOIUrl":"https://doi.org/10.1117/12.2508611","url":null,"abstract":"Polarized light microscopy and polarimetry has been used to assess changes in cervical structure by targeting its collagen. 75% of the human cervix is in fact composed of highly arranged collagen which is birefringent. Recently we have used Mueller Matrix polarimetry to image the human cervix in-vivo to determine loss of collagen arrangement associated with later stages of pregnancy. In an effort to improve our system capability and better determine the provenance of the polarized signal we have developed a Polarized Light Monte Carlo model capable of characterizing polarized light interaction with a birefringent, scattering, and absorbing medium such as the cervix. We have utilized this model to investigate the effect of cervical collagen arrangement typical of late stage in pregnancy on polarized light. In this talk we will illustrate the model framework, its validations, and provide several test cases in model cervices.","PeriodicalId":157928,"journal":{"name":"Optical Interactions with Tissue and Cells XXX","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116028571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Haudenschild, Xiangnan Zhou, Cai Li, Jerry C. Hu, J. K. Leach, K. Athanasiou, L. Marcu
{"title":"Multimodal evaluation of tissue engineered cartilage maturation in a pre-clinical implantation model (Conference Presentation)","authors":"A. Haudenschild, Xiangnan Zhou, Cai Li, Jerry C. Hu, J. K. Leach, K. Athanasiou, L. Marcu","doi":"10.1117/12.2509047","DOIUrl":"https://doi.org/10.1117/12.2509047","url":null,"abstract":"","PeriodicalId":157928,"journal":{"name":"Optical Interactions with Tissue and Cells XXX","volume":"195 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131801102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Building long lived test phantoms that simulate the scattering characteristics of biological tissue is needed in for testing optical methods targeted at imaging through tissue. Test phantoms are needed as real tissue samples optical characteristics (scattering coefficients µs and anisotropy factor g) varies greatly between samples and change rapidly with time. Our ongoing work has created long term stable phantoms with lifetimes of more than 6 years and maintaining consistent optical characteristics which mimic skin characteristics. These stable test phantom are created by an intralipid-infused agar layers 1 to 6 mm thickness Varying the intralipid concentration allows control of the scattering parameters with typical values of µs = 20/cm, g = 0.95. By encapsulating the intralipid-infused agar within a clear polymer it stabilizes for long lifetimes and allows creation a varying thicknesses, scattering characteristics and shapes. To characterize these test phantom we developed an enhanced technique where the scattered light from a laser beam passing through the test phantom is captured using a 36x24mm digital camera sensor to capture. This gives over 6 million measurements over a +/- 12 degree range, with typically 20,000 measurements at 2300 angular bins of 0.005 deg. For analysis these measurements of scattering values at a wide range of angles used a Matlab program to identify the scattering center and the angular positions. Fitted scattering models extracted the µs and g parameters for each test phantom Consistent results were obtained using a Henyey-Greenstein two-term model, probably because the Agar and intralipid impacted the scattering separately.
为了测试针对组织成像的光学方法，需要建立长寿命的模拟生物组织散射特性的测试模型。由于真实组织样品的光学特性(散射系数µs和各向异性因子g)在样品之间变化很大，并且随时间变化很快，因此需要测试模型。我们正在进行的工作创造了长期稳定的幻影，寿命超过6年，并保持一致的光学特性，模仿皮肤特性。这些稳定的测试幻影是由脂内注入琼脂层1至6mm厚度创建的，改变脂内浓度可以控制散射参数，典型值为µs = 20/cm, g = 0.95。通过将脂质内注入的琼脂包裹在透明聚合物中，它可以长时间稳定使用，并允许创建不同的厚度，散射特性和形状。为了表征这些测试模体，我们开发了一种增强技术，使用36x24mm数字相机传感器捕获通过测试模体的激光束散射光。这在+/- 12度范围内提供了超过600万次测量，通常在0.005度的2300角桶处进行20,000次测量。为了分析这些在宽角度范围内散射值的测量，使用Matlab程序来识别散射中心和角度位置。拟合的散射模型提取了每个测试幻影的µs和g参数，使用Henyey-Greenstein两项模型得到了一致的结果，可能是因为琼脂和脂肪内分别影响了散射。
{"title":"Long lived intralipid-infused tissue phantoms: control and characterization of scattering characteristics (Conference Presentation)","authors":"G. Chapman, Magda G. Sanchez","doi":"10.1117/12.2511052","DOIUrl":"https://doi.org/10.1117/12.2511052","url":null,"abstract":"Building long lived test phantoms that simulate the scattering characteristics of biological tissue is needed in for testing optical methods targeted at imaging through tissue. Test phantoms are needed as real tissue samples optical characteristics (scattering coefficients µs and anisotropy factor g) varies greatly between samples and change rapidly with time. Our ongoing work has created long term stable phantoms with lifetimes of more than 6 years and maintaining consistent optical characteristics which mimic skin characteristics. These stable test phantom are created by an intralipid-infused agar layers 1 to 6 mm thickness Varying the intralipid concentration allows control of the scattering parameters with typical values of µs = 20/cm, g = 0.95. By encapsulating the intralipid-infused agar within a clear polymer it stabilizes for long lifetimes and allows creation a varying thicknesses, scattering characteristics and shapes. To characterize these test phantom we developed an enhanced technique where the scattered light from a laser beam passing through the test phantom is captured using a 36x24mm digital camera sensor to capture. This gives over 6 million measurements over a +/- 12 degree range, with typically 20,000 measurements at 2300 angular bins of 0.005 deg. For analysis these measurements of scattering values at a wide range of angles used a Matlab program to identify the scattering center and the angular positions. Fitted scattering models extracted the µs and g parameters for each test phantom Consistent results were obtained using a Henyey-Greenstein two-term model, probably because the Agar and intralipid impacted the scattering separately.","PeriodicalId":157928,"journal":{"name":"Optical Interactions with Tissue and Cells XXX","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124688627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
X. Peralta, Jody C. Cantu, Cesario Z. Cerna, Morgan S. Schmidt, I. Echchgadda
{"title":"Influence of magnetic field exposure on epigenetic regulation in human keratinocytes (Conference Presentation)","authors":"X. Peralta, Jody C. Cantu, Cesario Z. Cerna, Morgan S. Schmidt, I. Echchgadda","doi":"10.1117/12.2508855","DOIUrl":"https://doi.org/10.1117/12.2508855","url":null,"abstract":"","PeriodicalId":157928,"journal":{"name":"Optical Interactions with Tissue and Cells XXX","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128139513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Quantifying optical properties of multi-layered human head model from in-vivo spatially resolved near-infrared spectra (Conference Presentation)","authors":"Ting-Xuan Lin, K. Sung","doi":"10.1117/12.2510963","DOIUrl":"https://doi.org/10.1117/12.2510963","url":null,"abstract":"","PeriodicalId":157928,"journal":{"name":"Optical Interactions with Tissue and Cells XXX","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132280377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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