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Drain biofilm microbiota inside a cheese processing facility: Comparison of Listeria positive and Listeria negative drains 奶酪加工厂内的排水沟生物膜微生物群:李斯特菌阳性排水沟与李斯特菌阴性排水沟的比较
Pub Date : 2023-12-06 DOI: 10.51337/jasb20231206005
Miriam Vesela
Listeria monocytogenes was identified as one of the most dangerous food-borne pathogens of the 21st century, not only due to its high mortality rate but also because of its ability to establish itself as a part of drain biofilms and persist there over long periods of time. The insides of twelve drains in a cheese processing facility were swabbed over a period of five months. Bacterial DNA was isolated and quantified to ascertain the presence and abundance of the biofilm. Presence of Listeria spp. prs gene was found in four of the twelve drains, with one drain testing positive for the prs gene presence on three different occasions. 16S rRNA amplicon sequencing was conducted on the isolated DNA from two Listeria positive and two Listeria negative drains. The microbial community composition was evaluated. Most notably genera Streptococci and unclassified Lactobacillales were significantly different in abundance in the Listeria negative drains. Genera Flavobacterium and unclassified Xanthomonadaceae were found to be significantly more different in abundance in the LisTeria positive drains. The latter two genera were not previously associated with Listeria spp. or reported to be widespread in food processing environments (FPE), however, they help to illustrate the higher diversity that was seen in Listeria positive drains. This study suggests that even though the alpha diversity is not sigN ificantly different, there are differences between the drain biofilm composition between Listeria positive and Listeria negative drains, which can be further researched to ascertain how these organisms affect the presence of Listeria spp. inside drain biofilms.
单核细胞增生李斯特菌被确定为 21 世纪最危险的食源性病原体之一,这不仅是因为它的死亡率很高,还因为它能够在下水道生物膜中形成自己的一部分并长期存在。在五个月的时间里,我们对一家奶酪加工厂的十二个下水道内部进行了抽样检查。对细菌 DNA 进行了分离和量化,以确定生物膜的存在和数量。在 12 个下水道中的 4 个发现了李斯特菌属 prs 基因,其中一个下水道的 prs 基因在 3 个不同场合的检测中均呈阳性。对从两个李斯特菌阳性和两个李斯特菌阴性排水沟中分离出的 DNA 进行了 16S rRNA 扩增片段测序。对微生物群落组成进行了评估。最明显的是链球菌属和未分类的乳酸杆菌属在李斯特菌阴性排水沟中的丰度有显著差异。在李斯特菌阳性的排水沟中,黄杆菌属和未分类的黄单胞菌属在丰度上有显著差异。后两个属以前与李斯特菌属没有关联,也没有报道说它们广泛存在于食品加工环境(FPE)中,但它们有助于说明李斯特菌阳性排水沟中更高的多样性。这项研究表明,尽管α多样性没有显著差异,但李斯特菌阳性排水沟和李斯特菌阴性排水沟的排水沟生物膜组成存在差异,可以对此进行进一步研究,以确定这些生物如何影响排水沟生物膜中李斯特菌属的存在。
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引用次数: 0
Magnetic field simulations of the GOLEM tokamak via the NICE code 通过 NICE 代码模拟 GOLEM 托卡马克磁场
Pub Date : 2023-12-06 DOI: 10.51337/jasb20231206003
Matyas Pokorny, Petr Macha, Vojtech Svoboda
In this paper, the direct and inverse equilibrium computation mode of the NICE code is put into operation for the case of the GOLEM tokamak. This enables, for the first time, simulations of GOLEM's magnetic field. The computation mode put into operation simulates the magnetohydrodynamic equilibrium of a tokamak plasma based on a given plasma position or given currents in poloidal magnetic field coils. In order to set up NICE, a virtual model of GOLEM is introduced that includes axially symmetrical approximations of GOLEM's iron core and primary transformer coils. The results of NICE simulations are compared with experimental measurements, and it is shown that NICE simulations produce plasma equilibrium configurations typical for GOLEM. Nevertheless, a more detailed validation of GOLEM's virtual model and of the results of NICE simulations is to be performed in the future.
在本文中,NICE 代码的直接和反平衡计算模式被用于 GOLEM 托卡马克的情况。这首次实现了对 GOLEM 磁场的模拟。投入运行的计算模式根据给定的等离子体位置或给定的极性磁场线圈中的电流模拟托卡马克等离子体的磁流体动力学平衡。为了建立 NICE,引入了 GOLEM 的虚拟模型,其中包括 GOLEM 铁芯和初级变压器线圈的轴对称近似值。NICE 模拟的结果与实验测量结果进行了比较,结果表明 NICE 模拟产生了典型的 GOLEM 等离子体平衡配置。不过,今后还将对 GOLEM 的虚拟模型和 NICE 模拟结果进行更详细的验证。
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引用次数: 0
Towards the intellectual compatibilism 走向思想上的兼容并包
Pub Date : 2023-12-06 DOI: 10.51337/jasb20231206002
Benedikt Mrazek
In present thesis, the author deals with the problem of free will and the possibility of its existence. He discovers the falsity of libertarianism radically conceived, according to which (radical) indeterminism is required for free will. But if we show that radical indeterminism is false, and if we deny any type of compatibilism as well, a logical dilemma once called an "Ancient Dilemma" enters the scene: If neither determinism nor indeterminism is consistent with free will, then free will simply cannot exist. The author provides a solution for this dilemma, while attempting to prove human freedom consistent. As he believes, however, this cannot be done until some important distinctions are provided, especially between External and Internal Determinism. While the author refutes External Determinism (and External Compatibilism), he defends Internal (or Intellectual) Determinism (as well as respective compatibilism). In the ending passage of his treatment, the author compares his philosophical solution of the free will problem with some recent scientific theories, especially with the physicalistically concieved Law of Conservation of Energy.
在本论文中,作者探讨了自由意志问题及其存在的可能性。他从根本上揭示了自由主义的虚假性,根据自由主义,自由意志需要(激进的)不确定性。但是,如果我们证明激进的不决定论是错误的,如果我们也否认任何类型的兼容并包论,那么一个曾被称为 "古代困境 "的逻辑困境就会出现:如果决定论和非决定论都不符合自由意志,那么自由意志就根本不可能存在。作者为这一难题提供了一个解决方案,同时试图证明人类的自由是一致的。然而,正如他所认为的那样,要做到这一点,就必须提供一些重要的区别,尤其是外部决定论和内部决定论之间的区别。作者在驳斥外在决定论(和外在相容论)的同时,为内在(或智力)决定论(以及各自的相容论)辩护。在论述的结尾部分,作者将其对自由意志问题的哲学解决方案与一些最新的科学理论,尤其是物理主义的 "能量守恒定律 "进行了比较。
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引用次数: 0
Unnatural Elements in Works of Tawada Yōko and Ogawa Yōko 俵田洋光和小川洋光作品中的非自然元素
Pub Date : 2023-12-06 DOI: 10.51337/jasb20231206001
Marcel Konicek
A woman growing scales which must be removed using skin-care products, or a man whose body disintegrates into dust after he left an elevator where he had spent his whole life. Modern Japanese literature is full of elements that run against our everyday experience such as these and writings of female writers Tawada Yōko and Ogawa Yōko are especially inundated by disconcerting and puzzling dreamlike scenes. What meaning are they trying to convey? What function does their inclusion in the text fulfil? How do they influence structure of their worlds? These questions pose a vexing problem for anyone attempting to analyse many of the works of contemporary Japanese literature as it is not easy to find a suitable theoretical approach to answer them. In this paper through interpretation of two shorter works by both authors E.B.'s Unfulfilled Wish by Ogawa and The Bath by Tawada) using a theoretical framework based on unnatural narratology and fictional world theory I attempt to answer these questions and show the central role these elements fulfil in both world-formation and meaning-formation of the texts. I will also point out how the usage of unnatural elements places both authors firmly into the realm of the ontology-dominated postmodern literature as defined by Brian McHale.
一个女人身上长出了必须用护肤品才能去除的鳞片,或者一个男人离开了他度过一生的电梯后,身体分解成了灰尘。日本现代文学中充斥着诸如此类违背我们日常经验的元素,女作家俵田洋子和小川洋子的作品中尤其充斥着令人不安和困惑的梦境。它们想表达什么意思?将它们写入文本有什么作用?它们如何影响其世界的结构?这些问题给试图分析许多日本当代文学作品的人带来了困扰,因为要找到合适的理论方法来回答这些问题并不容易。在本文中,我将运用基于非自然叙事学和虚构世界理论的理论框架,通过对两位作家的两部短篇作品(小川的《E.B.未了的心愿》和俵田的《洗澡》)的解读,尝试回答这些问题,并展示这些元素在文本的世界形成和意义形成中所发挥的核心作用。我还将指出,非自然元素的使用如何将两位作者牢牢地置于布赖恩-麦克黑尔所定义的以本体论为主导的后现代文学领域。
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引用次数: 0
Azo dyes synthesis demonstrations for education purposes 用于教育目的的偶氮染料合成演示
Pub Date : 2023-12-06 DOI: 10.51337/jasb20231206004
Vaclav Verner
Practical organic chemistry is one of the least accessible subjects to study in high school laboratories, due to high cost demands on organic chemicals and equipement. The objective of this project was to design and carry out an easily replicable synthesis for students to try out or for educators to demonstrate. The resulting 4-step 5-reaction synthesis, which uses well-known as well as alternative reactions, but only cheap and accessible reagents produces a diazonium dye which can be demonstrated by its pH-measuring properties.
由于对有机化学品和设备的高成本要求,实用有机化学是高中实验室中最不容易学习的科目之一。本项目的目标是设计并实施一种易于复制的合成方法,供学生尝试或教育工作者演示。由此产生的 4 步 5 反应合成法,既使用了众所周知的反应,也使用了替代反应,但只使用了廉价易得的试剂,生产出一种重氮染料,并可通过其 pH 测量特性加以证明。
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引用次数: 0
Observations of Recent Novae Visible on the Northern Hemisphere 北半球最近可见新星的观测
Pub Date : 2022-12-06 DOI: 10.51337/jasb20221206004
P. Dubovský, I. Kudzej, Tomáš Medulka, Petrik Karol, S. Shugarov
We are experiencing an increased rate of nova explosion observed from the northern hemisphere in the recent 2 years. Starting from July 2020 with the strongly reddened nova V1391 Cas, we are continuously observing some novae in outburst. Here, we report the observations of 5 classical novae: Nova Cas 2020 = V1391 Cas, Nova Per 2020 = V1112 Per, Nova Cas 2021 = V1405 Cas, Nova Her 2021 = V1674 Her and Nova Vul 2021 = V606 Vul. The best at the end - the recurrent symbiotic nova RS Oph. In this paper, we report our photometric and spectroscopic observations. Thorough analysis is to be done in the near future. At this moment we want to highlight two important facts. In the case of V1405 Cas, we know the orbital period of the progenitor. The progenitor of V1674 Her is an intermediate polar with known white dwarf spin period. In both cases, it will be very interesting to compare the values after the nova explosion. In the case of V1674 Her, we have already measured both the orbital and the spin period.
我们正在经历最近两年从北半球观测到的新星爆炸率的增加。从2020年7月强烈变红的新星V1391 Cas开始,我们连续观测到一些新星的爆发。本文报道了5颗经典新星的观测结果:Nova Cas 2020 = V1391 Cas, Nova Per 2020 = V1112 Per, Nova Cas 2021 = V1405 Cas, Nova Her 2021 = V1674 Her和Nova Vul 2021 = V606 Vul。最好的在最后-复发共生新星RS蛇夫。在本文中,我们报告了我们的光度和光谱观测结果。在不久的将来要进行彻底的分析。此时此刻,我们想强调两个重要的事实。在V1405 Cas的例子中,我们知道其前身的轨道周期。V1674 Her的前身是一个具有已知白矮星自旋周期的中间极。在这两种情况下,比较新星爆炸后的数值将是非常有趣的。在V1674 Her的例子中,我们已经测量了轨道和自旋周期。
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引用次数: 0
Period-luminosity relation of delta Sct stars from OGLE-IV and the CzeV catalogues OGLE-IV星表和CzeV星表中δ Sct星的周期光度关系
Pub Date : 2022-12-06 DOI: 10.51337/jasb20221206003
Marco Souza de Joode
In this article, the period-luminosity (P-L) relation is determined of delta Sct stars, based on a strictly selected sample of 483 stars from the OGLE-IV Galactic bulge fileds, together with 547 delta Sct, of which 52 are high-amplitude delta Sct stars (HADS) from the CzeV catalogue using Gaia DR2 parallaxes and apparent magnitudes. Absolute magnitudes were corrected for interstellar extinction using the Planck thermal foreground dust maps and the 3D Bayestar probabilistic dust map. Where possible, the CzeV catalogue periods were compared with photometric measurements from the TESS mission.
本文利用Gaia DR2视差和视星等,从OGLE-IV星系隆起场中严格选取483颗恒星和547颗δ Sct恒星,确定了δ Sct恒星的周期-光度(P-L)关系,其中52颗是来自CzeV星表的高振幅δ Sct恒星。使用普朗克热前景尘埃图和3D Bayestar概率尘埃图对绝对星等进行了星际消光校正。在可能的情况下,将CzeV目录周期与TESS任务的光度测量结果进行比较。
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引用次数: 0
Orbital period of the symbiotic system AX Persei determined by the method of radial velocities 由视向速度法确定的共生系统AX英仙座的轨道周期
Pub Date : 2022-12-06 DOI: 10.51337/jasb20221206002
P. Mártonfi, Galis Rudolf
AX Persei belongs to a group of symbiotic stars, which are open binaries usually consisting of a cool giant and a white dwarf. The mass transfer between the components of these interacting systems is responsible for the manifestations of their observed outburst activity. In this article, we present the results of determining the orbital period of AX Persei based on the analysis of its radial velocity curve. We used 75 radial velocities, out of which 45 were collected from literature and 30 were derived using the spectra from ARAS database. Two methods were used to determine the radial velocities: the Doppler shift measurement of the sodium absorption doublet and the cross-correlation analysis. The result of the period analysis of the radial velocities of AX Persei is the orbital period of 681.2 ± 4.2days, which is in good agreement with the value determined by our previous analysis of photometric variability of the symbiotic system as well as with the values derived by other researchers.
英仙座AX属于一群共生恒星,它们是开放的双星,通常由一颗冷巨星和一颗白矮星组成。这些相互作用体系的组分之间的传质是它们所观察到的突出活动表现的原因。本文在分析英仙座AX星径向速度曲线的基础上,给出了确定其轨道周期的结果。我们使用了75个径向速度,其中45个来自文献,30个来自ARAS数据库的光谱。采用两种方法测定径向速度:钠吸收双极体的多普勒频移测量和相互关系分析。对英仙座AX星径向速度的周期分析结果为681.2±4.2天,这与我们之前对共生系统光度变异性的分析结果以及其他研究人员得出的结果吻合得很好。
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引用次数: 0
There is More Than Just One “Corona” That Can Kill Us All 可以杀死我们所有人的“冠状病毒”不止一种
Pub Date : 2022-12-06 DOI: 10.51337/jasb20221206001
M. Brchnelova
When most people hear the phrase "space exploration", they imagine preparing the colonisation of Mars, studying distant planets, searching for alien life or studying exotic black holes. Many times, however, mankind tends to leap before looking and focus on the exotic and the interesting instead of that which might not seem that special to most of us, but which which can be crucial for our survival. This way, many major and important issues in astrophysics - and sciences in general - can become overlooked and take very long to resolve. One such major issue in astrophysics is the behaviour of our closest star, the Sun. While we generally assume that we have a good idea about the most significant processes in the solar interior acting as its main energy source, our attempts at explaining the dynamics and structure of the solar atmosphere (the so-called corona) are still merely an educated guesswork at best. Due to several of the magnetohydrodynamic processes which we do not yet fully understand, the temperature of the solar atmosphere is very high compared to the layers underneath and reaches millions of Kelvins. Since this is where the major mass ejections occur, some of which might be directed towards the Earth causing geomagnetic storms, it is crucial that we start not only to fully understand these processes, but also to be able to accurately predict their outcomes. As will be shown in this text however, it is not only our limited understanding of the physics which prevents us from doing that; it is also the lack of computational resources. This paper firstly briefly discusses the basic physics behind the behaviour of the solar coronal plasma. Afterwards, it discusses the coronal heating problem in more detail and finally, it outlines the major challenges we currently face which seem to prevent us from efficient simulation and complete understanding of the behaviour of our closest star.
当大多数人听到“太空探索”这个词时,他们想象的是准备殖民火星,研究遥远的行星,寻找外星生命或研究奇异的黑洞。然而,很多时候,人类倾向于在关注和关注异国情调和有趣的事物之前跳跃,而不是那些对我们大多数人来说似乎并不特别,但对我们的生存至关重要的东西。这样,天体物理学和一般科学中的许多重大问题都可能被忽视,需要很长时间才能解决。天体物理学中的一个主要问题就是离我们最近的恒星——太阳的行为。虽然我们通常认为我们对作为太阳主要能量来源的太阳内部最重要的过程有很好的了解,但我们试图解释太阳大气层(所谓的日冕)的动力学和结构,充其量也只是一种有根据的猜测。由于我们尚未完全了解的几个磁流体动力学过程,太阳大气的温度与下面的层相比非常高,达到数百万开尔文。由于这是主要的物质抛射发生的地方,其中一些可能直接指向地球导致地磁风暴,因此我们不仅要开始充分了解这些过程,而且要能够准确预测它们的结果,这一点至关重要。然而,正如本文将展示的那样,不仅是我们对物理学的有限理解阻碍了我们这样做;还有就是缺乏计算资源。本文首先简要讨论了太阳日冕等离子体行为背后的基本物理学。之后,它更详细地讨论了日冕加热问题,最后,它概述了我们目前面临的主要挑战,这些挑战似乎阻止了我们有效地模拟和完全理解我们最近的恒星的行为。
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引用次数: 0
Impact of the Economic Impact Payments on Consumer Spending: Analysis on a Granular Level of American Counties 经济影响支付对消费者支出的影响:基于美国郡县粒度水平的分析
Pub Date : 2021-12-27 DOI: 10.51337/jasb20211227002
Otakar Kořínek
This paper draws on weekly consumer spending data collected by American private companies to analyze the impact of the Economic Impact Payments on consumer spending in American counties. We use regression discontinuity design to quantify the causal effect of the Stimulus Checks on spending and use heterogeneity in economic and demographic factors to determine which groups of counties increased their spending the most, to see what factors affected the Stimulus Checks’ effectiveness. We then use the observed difference in impact across groups of counties to discuss whether the Stimulus Checks were the optimal governmental policy in the crisis and discuss the effectiveness of one-time transfer payments in future recessions.
本文利用美国私营公司收集的每周消费者支出数据来分析经济影响支付对美国各县消费者支出的影响。我们使用回归不连续设计来量化刺激检查对支出的因果效应,并使用经济和人口因素的异质性来确定哪些县的支出增加最多,以了解哪些因素影响刺激检查的有效性。然后,我们利用观察到的县组之间的影响差异来讨论刺激检查是否是危机中最优的政府政策,并讨论一次性转移支付在未来衰退中的有效性。
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引用次数: 0
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Journal of the ASB Society
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