Pub Date : 2023-12-01Epub Date: 2023-10-09DOI: 10.1080/08941939.2023.2266758
Zhuan Deng, Zhi-Sheng Long, Gang Chen
Background: Tendon-exposed wounds are complex injuries with challenging reconstructions and no unified treatment mode. Furthermore, insufficient tissue volume and blood circulation disorders affect healing, which increases pain for the patient and affects their families and caretakers.
Review: As modern medicine advances, considerable progress has been made in understanding and treating tendon-exposed wounds, and current research encompasses both macro-and micro-studies. Additionally, new treatment methods have emerged alongside the classic surgical methods, such as new dressing therapies, vacuum sealing drainage combination therapy, platelet-rich plasma therapy, and live-cell bioengineering.
Conclusions: This review summarizes the latest treatment methods for tendon-exposed wounds to provide ideas and improve their treatment.
{"title":"Mini-Review: Tendon-Exposed Wound Treatments.","authors":"Zhuan Deng, Zhi-Sheng Long, Gang Chen","doi":"10.1080/08941939.2023.2266758","DOIUrl":"10.1080/08941939.2023.2266758","url":null,"abstract":"<p><strong>Background: </strong>Tendon-exposed wounds are complex injuries with challenging reconstructions and no unified treatment mode. Furthermore, insufficient tissue volume and blood circulation disorders affect healing, which increases pain for the patient and affects their families and caretakers.</p><p><strong>Review: </strong>As modern medicine advances, considerable progress has been made in understanding and treating tendon-exposed wounds, and current research encompasses both macro-and micro-studies. Additionally, new treatment methods have emerged alongside the classic surgical methods, such as new dressing therapies, vacuum sealing drainage combination therapy, platelet-rich plasma therapy, and live-cell bioengineering.</p><p><strong>Conclusions: </strong>This review summarizes the latest treatment methods for tendon-exposed wounds to provide ideas and improve their treatment.</p>","PeriodicalId":16200,"journal":{"name":"Journal of Investigative Surgery","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41182787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-01Epub Date: 2023-10-18DOI: 10.1080/08941939.2023.2257780
Youdi Xue, Zhifa Lun, Jie Feng, Weixiang Dai
Purpose: To compare the clinical efficacy of percutaneous functional spinal unit cementoplasty (PFSUP) and posterior spinal fixation combined with vertebroplasty (PSF + VP) for the treatment of symptomatic chronic osteoporotic vertebral fractures (SCOVFs).
Method: Thirty-one patients with SCOVFs were included in this retrospective study and divided into PFSUP (n = 14) and PSF + VP (n = 17) groups. Visual analog scores (VAS) and Oswestry Disability Index (ODI) were recorded before and after surgery and at the last follow-up. Besides, the local kyphosis angle (LKA) and sagittal vertical axis (SVA) were measured. The operation duration, number of X-ray exposures, amount of blood loss, bed rest duration, hospitalization duration, and presence of complications were recorded.
Result: The VAS, ODI, LKA, and SVA after surgery and at the last follow-up were significantly improved in both groups compared to preoperative measurements. The PFSUP group experienced shorter operation duration (78.2 ± 13.1 vs. 124.7 ± 14.7, p < 0.001), less blood loss (31.1 ± 8.1 vs. 334.7 ± 70.9, p < 0.001), more X-ray exposures (92.1 ± 14.3 vs. 29.4 ± 5.5, p < 0.001), shorter bed rest duration (12.4 ± 3.8 vs. 43.4 ± 10.0, p < 0.001), shorter hospitalization (6.6 ± 2.4 vs. 10.9 ± 2.7, p < 0.001), lower complication rate (28.5% vs. 64.7%, p < 0.05), and higher cement leakage rate (42.9% vs. 5.8%, p < 0.05) than the PSF + VP group.
Conclusion: During the treatment of SCOVFs, the combination of PFSUP and PSF + VP can restore spinal stability, improve kyphosis, and relieve pain. PFSUP can reduce blood loss and complications, early mobilization, and shorten the hospital stay, but it is associated with a higher cement leakage rate and more radiation exposure.
目的:比较经皮功能性椎体单位牙骨质成形术(PFSUP)与脊柱后固定结合椎体成形术(PSF)的临床疗效 + VP)治疗有症状的慢性骨质疏松性椎体骨折(SCOVFs) = 14) 和PSF + VP(n = 17) 小组。术前、术后和最后一次随访时记录视觉模拟评分(VAS)和奥斯韦斯特里残疾指数(ODI)。测量局部后凸角(LKA)和矢状垂直轴(SVA)。记录手术时间、X光照射次数、失血量、卧床时间、住院时间和并发症的存在。结果:与术前测量相比,两组患者术后和最后一次随访时的VAS、ODI、LKA和SVA均有显著改善。PFSUP组的手术持续时间较短(78.2 ± 13.1对124.7 ± 14.7,p p p p p p p 结论:在治疗SCOVFs的过程中,PFSUP和PSF联合应用 + VP可以恢复脊柱稳定性,改善后凸,减轻疼痛。PFSUP可以减少失血和并发症,早期动员,缩短住院时间,但它与更高的水泥渗漏率和更多的辐射暴露有关。
{"title":"Percutaneous Functional Spinal Unit Cementoplasty versus Posterior Spinal Fixation with Vertebroplasty for Symptomatic Chronic Osteoporotic Vertebral Fractures: A Retrospective Study with a 2-Year Follow up.","authors":"Youdi Xue, Zhifa Lun, Jie Feng, Weixiang Dai","doi":"10.1080/08941939.2023.2257780","DOIUrl":"10.1080/08941939.2023.2257780","url":null,"abstract":"<p><strong>Purpose: </strong>To compare the clinical efficacy of percutaneous functional spinal unit cementoplasty (PFSUP) and posterior spinal fixation combined with vertebroplasty (PSF + VP) for the treatment of symptomatic chronic osteoporotic vertebral fractures (SCOVFs).</p><p><strong>Method: </strong>Thirty-one patients with SCOVFs were included in this retrospective study and divided into PFSUP (<i>n</i> = 14) and PSF + VP (<i>n</i> = 17) groups. Visual analog scores (VAS) and Oswestry Disability Index (ODI) were recorded before and after surgery and at the last follow-up. Besides, the local kyphosis angle (LKA) and sagittal vertical axis (SVA) were measured. The operation duration, number of X-ray exposures, amount of blood loss, bed rest duration, hospitalization duration, and presence of complications were recorded.</p><p><strong>Result: </strong>The VAS, ODI, LKA, and SVA after surgery and at the last follow-up were significantly improved in both groups compared to preoperative measurements. The PFSUP group experienced shorter operation duration (78.2 ± 13.1 vs. 124.7 ± 14.7, <i>p</i> < 0.001), less blood loss (31.1 ± 8.1 vs. 334.7 ± 70.9, <i>p</i> < 0.001), more X-ray exposures (92.1 ± 14.3 vs. 29.4 ± 5.5, <i>p</i> < 0.001), shorter bed rest duration (12.4 ± 3.8 vs. 43.4 ± 10.0, <i>p</i> < 0.001), shorter hospitalization (6.6 ± 2.4 vs. 10.9 ± 2.7, <i>p</i> < 0.001), lower complication rate (28.5% vs. 64.7%, <i>p</i> < 0.05), and higher cement leakage rate (42.9% vs. 5.8%, <i>p</i> < 0.05) than the PSF + VP group.</p><p><strong>Conclusion: </strong>During the treatment of SCOVFs, the combination of PFSUP and PSF + VP can restore spinal stability, improve kyphosis, and relieve pain. PFSUP can reduce blood loss and complications, early mobilization, and shorten the hospital stay, but it is associated with a higher cement leakage rate and more radiation exposure.</p>","PeriodicalId":16200,"journal":{"name":"Journal of Investigative Surgery","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49678389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-01Epub Date: 2023-10-09DOI: 10.1080/08941939.2023.2266736
Medhat Taha, Sara T Elazab, Ammar Qutub, Omer Abdelbagi, Tourki A S Baokbah, Gomaa S Ahmed, Randa A Zaghloul, Alaa Jameel A Albarakati, Naeem F Qusty, Omar Babateen, Abdullah G Al-Kushi
Background: Hyperglycemia usually impairs wound healing by dysregulating the inflammatory response and angiogenesis. This study aimed to examine the synergistic effect of dapagliflozin and Zamzam water (ZW) on the healing of diabetic wounds and to explore their anti-inflammatory and proangiogenic effects.Materials and methods: A full-thickness excisional wound was made on the backs of all groups after two weeks of diabetes induction. Forty rats were divided into five groups, with eight rats per group; Group 1: Control non-diabetic rats; Group II: Untreated diabetic rats; Group III: Diabetic rats drinking ZW; Group IV: Diabetic rats receiving an oral dose of 1 mg/kg dapagliflozin; and Group V: Received both dapagliflozin and ZW. The healing of diabetic wounds was assessed by measuring wound closure, oxidative stress markers, immunohistochemical staining of NF-βB, VEGF, CD34, CD45, Ki-67, and eNOS, gene expression of MMP-9, TGF-β1, EGF-b1, FGF, and Col1A1, protein levels of TNFα, IL-1β, IL6, Ang II, and HIF-1α by ELISA assay, and histological examination with H & E and Masson's trichrome. Combined treatment with dapagliflozin and ZW significantly (p < 0.05) enhanced the wound closure and antioxidant enzyme level, with apparent histological improvement, and shortened the inflammatory stage of the diabetic wound by decreasing the level of inflammatory markers NF-κB, TNF-α, IL-1β, IL6, and CD45. Therefore, it improved angiogenesis markers VEGF, CD34, eNOS, EGF-β1, FGF, Ang II, and HIF-1α, increasing Ki-67 cellular proliferation. Moreover, it enhanced the remodeling stage by increasing MMP-2, TGF-β1, and Col1A1 levels compared to diabetic rats.
{"title":"Novel Insights about Synergistic Effect of Zamzam Water with SGL2 Inhibitors on Wound Healing in STZ-Induced Diabetic Rats: The Role of anti-Inflammatory and Proangiogenic Effects.","authors":"Medhat Taha, Sara T Elazab, Ammar Qutub, Omer Abdelbagi, Tourki A S Baokbah, Gomaa S Ahmed, Randa A Zaghloul, Alaa Jameel A Albarakati, Naeem F Qusty, Omar Babateen, Abdullah G Al-Kushi","doi":"10.1080/08941939.2023.2266736","DOIUrl":"10.1080/08941939.2023.2266736","url":null,"abstract":"<p><p><b>Background:</b> Hyperglycemia usually impairs wound healing by dysregulating the inflammatory response and angiogenesis. This study aimed to examine the synergistic effect of dapagliflozin and Zamzam water (ZW) on the healing of diabetic wounds and to explore their anti-inflammatory and proangiogenic effects.<b>Materials and methods:</b> A full-thickness excisional wound was made on the backs of all groups after two weeks of diabetes induction. Forty rats were divided into five groups, with eight rats per group; Group 1: Control non-diabetic rats; Group II: Untreated diabetic rats; Group III: Diabetic rats drinking ZW; Group IV: Diabetic rats receiving an oral dose of 1 mg/kg dapagliflozin; and Group V: Received both dapagliflozin and ZW. The healing of diabetic wounds was assessed by measuring wound closure, oxidative stress markers, immunohistochemical staining of NF-βB, VEGF, CD34, CD45, Ki-67, and eNOS, gene expression of MMP-9, TGF-β1, EGF-b1, FGF, and Col1A1, protein levels of TNFα, IL-1β, IL6, Ang II, and HIF-1α by ELISA assay, and histological examination with H & E and Masson's trichrome. Combined treatment with dapagliflozin and ZW significantly (<i>p</i> < 0.05) enhanced the wound closure and antioxidant enzyme level, with apparent histological improvement, and shortened the inflammatory stage of the diabetic wound by decreasing the level of inflammatory markers NF-κB, TNF-α, IL-1β, IL6, and CD45. Therefore, it improved angiogenesis markers VEGF, CD34, eNOS, EGF-β1, FGF, Ang II, and HIF-1α, increasing Ki-67 cellular proliferation. Moreover, it enhanced the remodeling stage by increasing MMP-2, TGF-β1, and Col1A1 levels compared to diabetic rats.</p>","PeriodicalId":16200,"journal":{"name":"Journal of Investigative Surgery","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41182788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-01Epub Date: 2023-10-12DOI: 10.1080/08941939.2023.2266732
Hong Chen, Ling-Fei Zhang, Ying Miao, Yun Xi, Xuefei Li, Mo-Fang Liu, Min Zhang, Biao Li
Objective: The inhibition of the Hippo pathway through targeting the Yes-associated protein (YAP) presents a novel and promising approach for treating tumors. However, the efficacy of YAP inhibitors in the context of breast cancer (BC) remains incompletely understood. Here, we aimed to investigate the involvement of YAP in BC's metabolic reprogramming and reveal the potential underlying mechanisms. To this end, we assessed the function of verteporfin (VP), a YAP-TEAD complex inhibitor, on the glycolytic activity of BC cells.
Methods: We evaluated the expression of YAP by utilizing immunohistochemistry (IHC) in BC patients who have undergone 18F-fluorodeoxyglucose positron emission tomography/computed tomography (18F-FDG PET/CT) prior to biopsy/surgery. We employed RNA immunoprecipitation (RIP) and fluorescent in situ hybridization (FISH) assays to assess the interaction between YAP mRNA and human antigen R (HuR) in BC cells. The biological importance of YAP in the metabolism and malignancy of BC was evaluated in vitro. Finally, the effect of VP on glycolysis was determined by using 18F-FDG uptake, glucose consumption, and lactate production assays.
Results: Our studies revealed that high expression of YAP was positively correlated with the maximum uptake value (SUVmax) determined by 18F-FDG PET/CT imaging in BC samples. Inhibition of YAP activity suppressed glycolysis in BC. The mechanism underlying this phenomenon could be the binding of YAP to HuR, which promotes glycolysis in BC cells. Treatment with VP effectively suppressed glycolysis induced by YAP overexpression in BC cells.
Conclusion: VP exhibited anti-glycolytic effect on BC cells, indicating its therapeutic value as an FDA-approved drug.
{"title":"Verteporfin Suppresses YAP-Induced Glycolysis in Breast Cancer Cells.","authors":"Hong Chen, Ling-Fei Zhang, Ying Miao, Yun Xi, Xuefei Li, Mo-Fang Liu, Min Zhang, Biao Li","doi":"10.1080/08941939.2023.2266732","DOIUrl":"10.1080/08941939.2023.2266732","url":null,"abstract":"<p><strong>Objective: </strong>The inhibition of the Hippo pathway through targeting the Yes-associated protein (YAP) presents a novel and promising approach for treating tumors. However, the efficacy of YAP inhibitors in the context of breast cancer (BC) remains incompletely understood. Here, we aimed to investigate the involvement of YAP in BC's metabolic reprogramming and reveal the potential underlying mechanisms. To this end, we assessed the function of verteporfin (VP), a YAP-TEAD complex inhibitor, on the glycolytic activity of BC cells.</p><p><strong>Methods: </strong>We evaluated the expression of YAP by utilizing immunohistochemistry (IHC) in BC patients who have undergone <sup>18</sup>F-fluorodeoxyglucose positron emission tomography/computed tomography (<sup>18</sup>F-FDG PET/CT) prior to biopsy/surgery. We employed RNA immunoprecipitation (RIP) and fluorescent <i>in situ</i> hybridization (FISH) assays to assess the interaction between <i>YAP</i> mRNA and human antigen R (HuR) in BC cells. The biological importance of YAP in the metabolism and malignancy of BC was evaluated <i>in vitro</i>. Finally, the effect of VP on glycolysis was determined by using <sup>18</sup>F-FDG uptake, glucose consumption, and lactate production assays.</p><p><strong>Results: </strong>Our studies revealed that high expression of YAP was positively correlated with the maximum uptake value (SUV<sub>max</sub>) determined by <sup>18</sup>F-FDG PET/CT imaging in BC samples. Inhibition of YAP activity suppressed glycolysis in BC. The mechanism underlying this phenomenon could be the binding of YAP to HuR, which promotes glycolysis in BC cells. Treatment with VP effectively suppressed glycolysis induced by YAP overexpression in BC cells.</p><p><strong>Conclusion: </strong>VP exhibited anti-glycolytic effect on BC cells, indicating its therapeutic value as an FDA-approved drug.</p>","PeriodicalId":16200,"journal":{"name":"Journal of Investigative Surgery","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41203316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-01DOI: 10.1080/08941939.2023.2216756
Shuo Jian, Dehua Kong, Jieli Tian
Background: Pancreatic carcinoma (PC) is a global health threat with a high death rate. miRNAs are implicated in tumor initiation and progression. This study explored the expression of miR-425-5p in PC patients and its correlation with tumor immune microenvironment (TIME).Method: miR-425-5p expression in cancer tissues and adjacent non-tumor tissues of PC patients was examined by RT-qPCR. The levels of immune cells and cytokines were measured by flow cytometry and ELISA. The correlation of miR-425-5p with TNM stage and TIME was assessed by Spearman method. The death of PC patients was recorded through 36-month follow-ups. The prognosis of patients was assessed by Kaplan-Meier curves.Results: miR-425-5p expression was upregulated in PC tissues and elevated with increasing TNM stage. miR-425-5p expression was positively correlated with TNM stage. The PC tissues had decreased levels of CD3+, CD4+, CD8+, and natural killer (NK) cells, CD4+/CD8+ ratio, IL-2, and INF-γ, but increased levels of Tregs, IL-4, IL-10, and TGF-β. miR-425-5p level in cancer tissues was positively correlated with Tregs/IL-10/TGF-β, but negatively related to CD3+/CD4+/CD8+/NK cells and IL-2/INF-γ. Moreover, high miR-425-5p expression predicted a poor prognosis in PC patients.Conclusion: miR-425-5p is upregulated in PC patients and is prominently associated with the TIME, and high miR-425-5p predicts a poor prognosis in PC patients.
{"title":"Expression of miR-425-5p in Pancreatic Carcinoma and Its Correlation with Tumor Immune Microenvironment.","authors":"Shuo Jian, Dehua Kong, Jieli Tian","doi":"10.1080/08941939.2023.2216756","DOIUrl":"10.1080/08941939.2023.2216756","url":null,"abstract":"<p><p><b>Background:</b> Pancreatic carcinoma (PC) is a global health threat with a high death rate. miRNAs are implicated in tumor initiation and progression. This study explored the expression of miR-425-5p in PC patients and its correlation with tumor immune microenvironment (TIME).<b>Method:</b> miR-425-5p expression in cancer tissues and adjacent non-tumor tissues of PC patients was examined by RT-qPCR. The levels of immune cells and cytokines were measured by flow cytometry and ELISA. The correlation of miR-425-5p with TNM stage and TIME was assessed by Spearman method. The death of PC patients was recorded through 36-month follow-ups. The prognosis of patients was assessed by Kaplan-Meier curves.<b>Results:</b> miR-425-5p expression was upregulated in PC tissues and elevated with increasing TNM stage. miR-425-5p expression was positively correlated with TNM stage. The PC tissues had decreased levels of CD3+, CD4+, CD8+, and natural killer (NK) cells, CD4+/CD8+ ratio, IL-2, and INF-γ, but increased levels of Tregs, IL-4, IL-10, and TGF-β. miR-425-5p level in cancer tissues was positively correlated with Tregs/IL-10/TGF-β, but negatively related to CD3+/CD4+/CD8+/NK cells and IL-2/INF-γ. Moreover, high miR-425-5p expression predicted a poor prognosis in PC patients.<b>Conclusion:</b> miR-425-5p is upregulated in PC patients and is prominently associated with the TIME, and high miR-425-5p predicts a poor prognosis in PC patients.</p>","PeriodicalId":16200,"journal":{"name":"Journal of Investigative Surgery","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10182925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-01Epub Date: 2023-12-04DOI: 10.1080/08941939.2023.2285780
Qirun Cheng, Lipeng Chen, Liping Ni
Objective: This study aims to investigate the association between miR-203 expression and the prognostic value in patients with esophageal cancer by the method of systematic review and meta-analysis.
Methods: We searched PubMed, Web of Science, Embase, and Cochrane Library to collect studies on the relationship between miR-203 expression and the prognostic value of esophageal cancer up to July 2023. Stata 15.0 statistical software was used for data analysis. Hazard ratio (HR) and 95% confidence interval (CI) were used as effect sizes.
Results: A total of 6 studies were included in this review, including 476 patients with esophageal cancer. The results showed that miR-203 low expression was associated with worse overall survival (OS) in patients with esophageal cancer compared with miR-203 high expression (HR = 2.80, 95%CI: 1.99 ∼ 3.93, p < 0.001). The results of Egger's (p = 0.154) and Begg's Tests (p = 0.221) indicated no obvious publication bias. Sensitivity analysis verified the robustness of the results obtained in this study.
Conclusion: The expression of miR-203 is significantly correlated with the prognostic value in patients with esophageal cancer. Esophageal cancer patients with high expression of miR-203 had better prognosis than those with low expression of miR-203. Due to the limited studies included in this meta-analysis, more trials are needed to confirm the conclusions of this study in the future.
目的:本研究旨在通过系统综述和荟萃分析的方法,探讨miR-203表达与食管癌患者预后价值的关系。方法:检索PubMed、Web of Science、Embase、Cochrane Library,收集截至2023年7月关于miR-203表达与食管癌预后价值关系的研究。采用Stata 15.0统计软件进行数据分析。采用风险比(HR)和95%置信区间(CI)作为效应量。结果:本综述共纳入6项研究,纳入476例食管癌患者。结果显示,与miR-203高表达患者相比,miR-203低表达与更差的食管癌患者总生存期(OS)相关(HR = 2.80, 95%CI: 1.99 ~ 3.93, p p = 0.154), Begg检验(p = 0.221)显示无明显的发表偏倚。敏感性分析验证了本研究结果的稳健性。结论:miR-203的表达与食管癌患者的预后价值显著相关。高表达miR-203的食管癌患者预后优于低表达miR-203的食管癌患者。由于本荟萃分析纳入的研究有限,未来需要更多的试验来证实本研究的结论。
{"title":"Association of miR-203 Expression with Prognostic Value in Patients with Esophageal Cancer: A Systematic Review and Meta-Analysis.","authors":"Qirun Cheng, Lipeng Chen, Liping Ni","doi":"10.1080/08941939.2023.2285780","DOIUrl":"10.1080/08941939.2023.2285780","url":null,"abstract":"<p><strong>Objective: </strong>This study aims to investigate the association between miR-203 expression and the prognostic value in patients with esophageal cancer by the method of systematic review and meta-analysis.</p><p><strong>Methods: </strong>We searched PubMed, Web of Science, Embase, and Cochrane Library to collect studies on the relationship between miR-203 expression and the prognostic value of esophageal cancer up to July 2023. Stata 15.0 statistical software was used for data analysis. Hazard ratio (HR) and 95% confidence interval (CI) were used as effect sizes.</p><p><strong>Results: </strong>A total of 6 studies were included in this review, including 476 patients with esophageal cancer. The results showed that miR-203 low expression was associated with worse overall survival (OS) in patients with esophageal cancer compared with miR-203 high expression (HR = 2.80, 95%CI: 1.99 ∼ 3.93, <i>p</i> < 0.001). The results of Egger's (<i>p</i> = 0.154) and Begg's Tests (<i>p</i> = 0.221) indicated no obvious publication bias. Sensitivity analysis verified the robustness of the results obtained in this study.</p><p><strong>Conclusion: </strong>The expression of miR-203 is significantly correlated with the prognostic value in patients with esophageal cancer. Esophageal cancer patients with high expression of miR-203 had better prognosis than those with low expression of miR-203. Due to the limited studies included in this meta-analysis, more trials are needed to confirm the conclusions of this study in the future.</p>","PeriodicalId":16200,"journal":{"name":"Journal of Investigative Surgery","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138477835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-08Epub Date: 2023-10-24DOI: 10.1080/08941939.2023.2257770
Bilian Zheng, Man Lu, Chuang Chen, Shengrong Sun
Purpose: Granulomatous lobular mastitis (GLM) is a benign inflammatory disease of the mammary gland with unknown etiology. Erythema nodosum (EN) is a rare, extramammary symptom of GLM. The purpose of this article was to investigate the clinical features of EN associated with GLM.
Methods: We recruited 102 GLM patients diagnosed between December 2018 and December 2021 at Renmin Hospital of Wuhan University. The clinical characteristics and laboratory indices of the EN group (n = 12) and the non-EN group (n = 90) were compared.
Results: The proportion of GLM patients with lesions involving ≥2 quadrants and high peripheral white blood cell count, absolute neutrophil count and neutrophil-to-lymphocyte ratio (NLR) was larger in the EN group than in the non-EN group (p = 0.002, 0.025, 0.014, 0.002, respectively). The duration of EN was longer in GLM patients with EN than in GLM patients without EN (p = 0.005). GLM patients with EN had more abscesses and sinus tracts than those without EN (p = 0.003, 0.038). Lesions involving ≥2 quadrants and the NLR were positively associated with the occurrence of EN (R = 0.304, 0.0302, p = 0.002, 0.002). Receiver operating characteristic curve analysis revealed that the area under the curve of the NLR was 0.770. When NLR > 5.73, the sensitivity and specificity of predicting EN were 66.67% and 87.78%, respectively.
Conclusion: Our findings suggest that GLM concomitant with EN suggests the presence of a more severe condition and extensive lesions.
{"title":"The Distinct Pattern of Granulomatous Lobular Mastitis with Erythema Nodosum: More Severe Conditions and Extensive Lesions.","authors":"Bilian Zheng, Man Lu, Chuang Chen, Shengrong Sun","doi":"10.1080/08941939.2023.2257770","DOIUrl":"10.1080/08941939.2023.2257770","url":null,"abstract":"<p><strong>Purpose: </strong>Granulomatous lobular mastitis (GLM) is a benign inflammatory disease of the mammary gland with unknown etiology. Erythema nodosum (EN) is a rare, extramammary symptom of GLM. The purpose of this article was to investigate the clinical features of EN associated with GLM.</p><p><strong>Methods: </strong>We recruited 102 GLM patients diagnosed between December 2018 and December 2021 at Renmin Hospital of Wuhan University. The clinical characteristics and laboratory indices of the EN group (<i>n</i> = 12) and the non-EN group (<i>n</i> = 90) were compared.</p><p><strong>Results: </strong>The proportion of GLM patients with lesions involving ≥2 quadrants and high peripheral white blood cell count, absolute neutrophil count and neutrophil-to-lymphocyte ratio (NLR) was larger in the EN group than in the non-EN group (<i>p</i> = 0.002, 0.025, 0.014, 0.002, respectively). The duration of EN was longer in GLM patients with EN than in GLM patients without EN (<i>p</i> = 0.005). GLM patients with EN had more abscesses and sinus tracts than those without EN (<i>p</i> = 0.003, 0.038). Lesions involving ≥2 quadrants and the NLR were positively associated with the occurrence of EN (<i>R</i> = 0.304, 0.0302, <i>p</i> = 0.002, 0.002). Receiver operating characteristic curve analysis revealed that the area under the curve of the NLR was 0.770. When NLR > 5.73, the sensitivity and specificity of predicting EN were 66.67% and 87.78%, respectively.</p><p><strong>Conclusion: </strong>Our findings suggest that GLM concomitant with EN suggests the presence of a more severe condition and extensive lesions.</p>","PeriodicalId":16200,"journal":{"name":"Journal of Investigative Surgery","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50158115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-01Epub Date: 2022-09-28DOI: 10.1080/08941939.2022.2128117
Erdal Uysal, Mehmet Dokur, Faruk Kucukdurmaz, Serdar Altınay, Sait Polat, Kadir Batcıoglu, Efe Sezgın, Tuğçe Sapmaz Erçakallı, Aslı Yaylalı, Yakup Yılmaztekin, Zafer Cetın, İlker Saygılı, Osman Barut, Hatem Kazımoglu, Gokturk Maralcan, Suna Koc, Turkan Guney, Nadire Eser, Mehmet Sökücü, Sema Nur Dokur
Objectives: The objectives of this study were a) to investigate the effect of targeting the PANoptosome with 3,4-methylenedioxy-β-nitrostyrene (MNS) on PANoptosis in the Renal ischemia-reperfussion (RIR) model b) to investigate the kidney protective effect of MNS toward RIR injury.
Methods: Thirty-two rats were divided into four groups randomly. The groups were assigned as Control, Sham, DMSO (dimethyl sulfoxide) and MNS groups. The rats in the MNS group were intraperitoneally given 20 mg/kg of MNS 30 minutes before reperfusion. 2% DMSO solvent that dissolves MNS were given to the rats in DMSO group. Left nephrectomy was performed on the rats under anesthesia at the 6th hour after reperfusion. Glutathione peroxidase (GPx), malondialdehyde (MDA), catalase (CAT), superoxide dismutase (SOD) and 8-Okso-2'-deoksiguanozin (8-OHdG) levels were measured. Immunohistochemical analysis, electron microscopic and histological examinations were carried out in the tissues.
Results: Total tubular injury score was lower in the MNS group (p < 0.001). Caspase-3, Gasdermin D and MLK (Mixed Lineage Kinase Domain Like Pseudokinase) expressions were considerably decreased in the MNS group (p < 0.001). Apoptotic index (AI) was found to be low in the MNS group (p < 0.001). CAT and SOD levels were higher in the MNS Group (p = 0.006, p = 0.0004, respectively). GPx, MDA, and 8-OH-dG levels were similar (p > 0.05) in all groups. MNS considerably improved the tissue structure, based on the electron microscopic analysis.
Conclusions: Our results suggested that MNS administrated before the reperfusion reduces pyroptosis, apoptosis and necroptosis. These findings suggest that MNS significantly protects the kidney against RIR injury by reducing PANoptosis as a result of specific inhibition of Nod-like receptor pyrin domain-containing 3 (NLRP 3), one of the PANoptosome proteins.
目的:本研究的目的是:a)研究3,4-亚甲二氧基-β-亚硝基苯乙烯(MNS)靶向泛视体对肾缺血再灌注(RIR)模型中泛视症的影响;b)研究MNS对RIR损伤的肾脏保护作用。方法:将32只大鼠随机分为4组。将各组分为对照组、Sham组、DMSO(二甲基亚砜)组和MNS组。MNS组大鼠腹腔注射20 mg/kg MNS 30 再灌注前几分钟。DMSO组给予大鼠2%溶解MNS的DMSO溶剂。再灌注后6h,在麻醉下对大鼠进行左肾切除术。测定谷胱甘肽过氧化物酶(GPx)、丙二醛(MDA)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)和8-Okso-2'-脱氧鸟苷(8-OHdG)水平。组织进行免疫组织化学分析、电镜和组织学检查。结果:MNS组肾小管损伤总分较低(p p p p = 0.006,p = 0.0004)。GPx、MDA和8-OH-dG水平相似(p > 0.05)。基于电子显微镜分析,MNS显著改善了组织结构。结论:再灌注前给予MNS可减少焦下垂、细胞凋亡和坏死。这些发现表明,MNS通过减少泛视症而显著保护肾脏免受RIR损伤,泛视症是由于对泛视体蛋白之一的含有Nod样受体pyrin结构域3(NLRP 3)的特异性抑制所致。
{"title":"Targeting the PANoptosome with 3,4-Methylenedioxy-β-Nitrostyrene, Reduces PANoptosis and Protects the Kidney against Renal İschemia-Reperfusion Injury.","authors":"Erdal Uysal, Mehmet Dokur, Faruk Kucukdurmaz, Serdar Altınay, Sait Polat, Kadir Batcıoglu, Efe Sezgın, Tuğçe Sapmaz Erçakallı, Aslı Yaylalı, Yakup Yılmaztekin, Zafer Cetın, İlker Saygılı, Osman Barut, Hatem Kazımoglu, Gokturk Maralcan, Suna Koc, Turkan Guney, Nadire Eser, Mehmet Sökücü, Sema Nur Dokur","doi":"10.1080/08941939.2022.2128117","DOIUrl":"10.1080/08941939.2022.2128117","url":null,"abstract":"<p><strong>Objectives: </strong>The objectives of this study were a) to investigate the effect of targeting the PANoptosome with 3,4-methylenedioxy-β-nitrostyrene (MNS) on PANoptosis in the Renal ischemia-reperfussion (RIR) model b) to investigate the kidney protective effect of MNS toward RIR injury.</p><p><strong>Methods: </strong>Thirty-two rats were divided into four groups randomly. The groups were assigned as Control, Sham, DMSO (dimethyl sulfoxide) and MNS groups. The rats in the MNS group were intraperitoneally given 20 mg/kg of MNS 30 minutes before reperfusion. 2% DMSO solvent that dissolves MNS were given to the rats in DMSO group. Left nephrectomy was performed on the rats under anesthesia at the 6th hour after reperfusion. Glutathione peroxidase (GPx), malondialdehyde (MDA), catalase (CAT), superoxide dismutase (SOD) and 8-Okso-2'-deoksiguanozin (8-OHdG) levels were measured. Immunohistochemical analysis, electron microscopic and histological examinations were carried out in the tissues.</p><p><strong>Results: </strong>Total tubular injury score was lower in the MNS group (<i>p</i> < 0.001). Caspase-3, Gasdermin D and MLK (Mixed Lineage Kinase Domain Like Pseudokinase) expressions were considerably decreased in the MNS group (<i>p</i> < 0.001). Apoptotic index (AI) was found to be low in the MNS group (<i>p</i> < 0.001). CAT and SOD levels were higher in the MNS Group (<i>p</i> = 0.006, <i>p</i> = 0.0004, respectively). GPx, MDA, and 8-OH-dG levels were similar (<i>p</i> > 0.05) in all groups. MNS considerably improved the tissue structure, based on the electron microscopic analysis.</p><p><strong>Conclusions: </strong>Our results suggested that MNS administrated before the reperfusion reduces pyroptosis, apoptosis and necroptosis. These findings suggest that MNS significantly protects the kidney against RIR injury by reducing PANoptosis as a result of specific inhibition of Nod-like receptor pyrin domain-containing 3 (NLRP 3), one of the PANoptosome proteins.</p>","PeriodicalId":16200,"journal":{"name":"Journal of Investigative Surgery","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40379854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-01Epub Date: 2022-10-06DOI: 10.1080/08941939.2022.2130481
Roberto Rasslan, Vinicios Alves, Sergio Henrique Bastos Damous, Antonio de Santis, Flavio Tarasoutchi, Calos Augusto Metidieri Menegozzo, Masahiko Akamine, Samir Rasslan, Edivaldo Massazo Utiyama
Introduction: Splenic abscess secondary to endocarditis is a rare complication with high mortality. The treatment modality, splenectomy versus percutaneous drainage, and the best time, before or after valve replacement, are controversial. In the literature, there are only a few small case series about the subject. The objective of this study is to analyze the experience of a referral center in treating such condition.
Methods: Patients with splenic abscesses due to endocarditis from 2006 to 2020 were retrospectively analyzed.
Results: Thirteen patients (mean age 46 years old, 69% male) were identified. Eight patients (62%) had at least 2 comorbidities and 5 (38%) had a history of cardiac surgery. The diagnosis was incidental in 6 (46%). The mean time of abscess diagnosis after endocarditis definition was 14 days. Six patients (46%) had at least two organ dysfunctions. The median APACHE II score was 12 overall, and 24.5 in patients who died. Six patients (46%) had a valve replacement, and in two the abscess was diagnosed postoperatively. Of the other four patients, splenectomy was performed before the cardiac operation in three and at the same time in one. Splenectomy was performed immediately in 9 (69%) patients while three patients had percutaneous drainage (23%), one of which underwent splenectomy due to drainage failure. Exclusive antibiotic treatment was performed on only one patient. The median length of hospitalization was 24 days and mortality was 46%.
Conclusion: Splenic abscess due to endocarditis is a life-threatening condition with controversial treatment that results in a prolonged length of stay and high mortality.
{"title":"Splenic Abscesses in Endocarditis: A Rare Disease with High Mortality. The Experience of a Heart Institute in Brazil.","authors":"Roberto Rasslan, Vinicios Alves, Sergio Henrique Bastos Damous, Antonio de Santis, Flavio Tarasoutchi, Calos Augusto Metidieri Menegozzo, Masahiko Akamine, Samir Rasslan, Edivaldo Massazo Utiyama","doi":"10.1080/08941939.2022.2130481","DOIUrl":"https://doi.org/10.1080/08941939.2022.2130481","url":null,"abstract":"<p><strong>Introduction: </strong>Splenic abscess secondary to endocarditis is a rare complication with high mortality. The treatment modality, splenectomy versus percutaneous drainage, and the best time, before or after valve replacement, are controversial. In the literature, there are only a few small case series about the subject. The objective of this study is to analyze the experience of a referral center in treating such condition.</p><p><strong>Methods: </strong>Patients with splenic abscesses due to endocarditis from 2006 to 2020 were retrospectively analyzed.</p><p><strong>Results: </strong>Thirteen patients (mean age 46 years old, 69% male) were identified. Eight patients (62%) had at least 2 comorbidities and 5 (38%) had a history of cardiac surgery. The diagnosis was incidental in 6 (46%). The mean time of abscess diagnosis after endocarditis definition was 14 days. Six patients (46%) had at least two organ dysfunctions. The median APACHE II score was 12 overall, and 24.5 in patients who died. Six patients (46%) had a valve replacement, and in two the abscess was diagnosed postoperatively. Of the other four patients, splenectomy was performed before the cardiac operation in three and at the same time in one. Splenectomy was performed immediately in 9 (69%) patients while three patients had percutaneous drainage (23%), one of which underwent splenectomy due to drainage failure. Exclusive antibiotic treatment was performed on only one patient. The median length of hospitalization was 24 days and mortality was 46%.</p><p><strong>Conclusion: </strong>Splenic abscess due to endocarditis is a life-threatening condition with controversial treatment that results in a prolonged length of stay and high mortality.</p>","PeriodicalId":16200,"journal":{"name":"Journal of Investigative Surgery","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33489850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: This study aimed to assess the predictive value of tumor regression grade (TRG) and nodal status on survival in esophageal carcinoma with neoadjuvant chemoradiotherapy (nCRT).
Methods: Tumor pathologic regression and nodal status were assessed. Differences in survival stratified by TRG or nodal status were analyzed using the Kaplan-Meier method and log-rank test. The prognostic value of TRG and nodal status were analyzed using univariate and multivariate Cox proportional hazards methods.
Results: From July 2016 to June 2019, 253 patients with esophageal cancer underwent nCRT followed by surgery. Significant differences were presented in survival according to nodal status but not TRG. Multivariate analysis showed that nodal status and not TRG was the only independent predicter for overall survival (HR: 3.550, 95% CI: 2.264-5.566, P < 0.001) and disease-free survival (HR: 2.801, 95% CI: 1.874-4.187, P < 0.001). The modified TRG system combining tumor regression with nodal status stratified patients survival with good discrimination.
Conclusions: Lymph node status impacts more importantly than TRG on survival for patients with esophageal cancer undergoing nCRT plus esophagectomy. The modified TRG system may facilitate postoperative treatment decisions and survival surveillance.
背景:本研究旨在评估肿瘤消退等级(TRG)和淋巴结状态对食管癌新辅助放化疗(nCRT)患者生存的预测价值。方法:观察肿瘤病理消退及淋巴结状态。采用Kaplan-Meier法和log-rank检验分析按TRG或淋巴结状态分层的生存差异。采用单因素和多因素Cox比例风险法分析TRG和淋巴结状态的预后价值。结果:2016年7月至2019年6月,253例食管癌患者接受了nCRT后手术治疗。不同淋巴结状态的生存率有显著差异,但TRG无显著差异。多因素分析显示,淋巴结状态而非TRG是总生存的唯一独立预测因素(HR: 3.550, 95% CI: 2.264-5.566, P P)。结论:淋巴结状态比TRG对食管癌行nCRT +食管切除术患者生存的影响更重要。改良后的TRG系统有助于术后治疗决策和生存监测。
{"title":"Is Tumor Regression Grade Sufficient to Predict Survival in Esophageal Cancer with Trimodal Therapy?","authors":"Yi-Min Gu, Si-Mian Lyu, Qi-Xin Shang, Han-Lu Zhang, Yu-Shang Yang, Wen-Ping Wang, Yong Yuan, Long-Qi Chen","doi":"10.1080/08941939.2022.2127036","DOIUrl":"https://doi.org/10.1080/08941939.2022.2127036","url":null,"abstract":"<p><strong>Background: </strong>This study aimed to assess the predictive value of tumor regression grade (TRG) and nodal status on survival in esophageal carcinoma with neoadjuvant chemoradiotherapy (nCRT).</p><p><strong>Methods: </strong>Tumor pathologic regression and nodal status were assessed. Differences in survival stratified by TRG or nodal status were analyzed using the Kaplan-Meier method and log-rank test. The prognostic value of TRG and nodal status were analyzed using univariate and multivariate Cox proportional hazards methods.</p><p><strong>Results: </strong>From July 2016 to June 2019, 253 patients with esophageal cancer underwent nCRT followed by surgery. Significant differences were presented in survival according to nodal status but not TRG. Multivariate analysis showed that nodal status and not TRG was the only independent predicter for overall survival (HR: 3.550, 95% CI: 2.264-5.566, <i>P</i> < 0.001) and disease-free survival (HR: 2.801, 95% CI: 1.874-4.187, <i>P</i> < 0.001). The modified TRG system combining tumor regression with nodal status stratified patients survival with good discrimination.</p><p><strong>Conclusions: </strong>Lymph node status impacts more importantly than TRG on survival for patients with esophageal cancer undergoing nCRT plus esophagectomy. The modified TRG system may facilitate postoperative treatment decisions and survival surveillance.</p>","PeriodicalId":16200,"journal":{"name":"Journal of Investigative Surgery","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40377639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}