Pub Date : 2023-06-22DOI: 10.1080/10826076.2023.2224024
Alina Tarna, C. Cimpoiu, A. Hosu
Abstract Honey is an important functional food due to its content of polyphenolic compounds with beneficial effects on human body. The analysis of polyphenolic compound in honey is very promising in determination of floral origin and so for quality control. This study approaches a new methodology using integrated methods for the determination of honey authenticity. Polyphenolic compounds from seven different types of monofloral honey from two different sources were extracted and analyzed by thin layer chromatography (TLC) and UV-Vis spectroscopy. Chromatographic analysis was performed on TLC Silica gel 60 RP-18 F254 plates using a mobile phase consisting in a mixture of methanol: water: formic acid (5: 5: 0.1, v/v/v). The images from the TLC analysis were processed and the obtained chromatograms together with the UV-Vis deconvoluted spectra of honey were subjected to visual and statistical analysis. The obtained results showed notable differences between the honey samples, which can be used to establish their floral origin and for their authentication. The proposed methodology is easy to perform, cost-effective and offers complementary information, therefore it has the potential of being used for the honey authentication. GRAPHICAL ABSTRACT
{"title":"Integrated methods for fingerprinting of monofloral honeys","authors":"Alina Tarna, C. Cimpoiu, A. Hosu","doi":"10.1080/10826076.2023.2224024","DOIUrl":"https://doi.org/10.1080/10826076.2023.2224024","url":null,"abstract":"Abstract Honey is an important functional food due to its content of polyphenolic compounds with beneficial effects on human body. The analysis of polyphenolic compound in honey is very promising in determination of floral origin and so for quality control. This study approaches a new methodology using integrated methods for the determination of honey authenticity. Polyphenolic compounds from seven different types of monofloral honey from two different sources were extracted and analyzed by thin layer chromatography (TLC) and UV-Vis spectroscopy. Chromatographic analysis was performed on TLC Silica gel 60 RP-18 F254 plates using a mobile phase consisting in a mixture of methanol: water: formic acid (5: 5: 0.1, v/v/v). The images from the TLC analysis were processed and the obtained chromatograms together with the UV-Vis deconvoluted spectra of honey were subjected to visual and statistical analysis. The obtained results showed notable differences between the honey samples, which can be used to establish their floral origin and for their authentication. The proposed methodology is easy to perform, cost-effective and offers complementary information, therefore it has the potential of being used for the honey authentication. GRAPHICAL ABSTRACT","PeriodicalId":16295,"journal":{"name":"Journal of Liquid Chromatography & Related Technologies","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49038966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Zibushengfa Tablet is a widely consumed traditional Chinese medicine (TCM) prescription. However, its main bioactives remain unclear and its pharmacopeia quality-marker (Q-marker) is poorly characterized nowadays. The present study analyzed its lyophilized aqueous powder using ultra-high-performance liquid chromatography-quadrupole-orbitrap mass spectrometry. Through comparison with authentic standards in the library, 46 bioactives were putatively identified, including quinic acid, D-gluconic acid, gallic acid, protocatechuic acid, 5-caffeoylquinic acid, L-tryptophan, salidroside, (+)-catechin hydrate, caffeic acid, cryptochlorogenic acid, mangiferin, ferulic acid, myricetin-3-O-galactoside, 2,3,5,4’-tetrahydroxystilbene-2-O-glucoside, forsythoside B, myricitrin, rutin, hyperoside, acteoside, 3,4-dicaffeoylquinic acid, myricetin, senkyunolide A, isochlorogenic acid C, quercitrin, (+)-epipinoresinol, quercetin, biochanin A, calycosin, luteolin, wedelolactone, calycosin-7-O-glucoside, chrysoeriol, trans-cinnamic acid, formononetin, chrysin, 3,3’,4’,5,6,7,8-heptamethoxyflavone, Z-ligustilide, amentoflavone, tangeretin, hinokiflavone, emodin, physcion, linoleic acid, stearic acid, palmitic acid, and palmitic acid ethyl ester. Of these, four pairs of isomers were successfully differentiated using the newly established library-filtering strategy. On this basis, senkyunolide A, amentoflavone, quercetin, quercitrin, and wedelolactone are preliminarily suggested as additional quality-markers for the consideration by Pharmacopeia Committee, to prevent the possible adulterate. Graphical Abstract
{"title":"Library-based UHPLC-Q-exactive-orbitrap-MS putative identification of isomeric and non-isomeric bioactives from Zibushengfa Tablet and pharmacopeia quality-marker chemistry","authors":"Shaoman Chen, Xican Li, Jingyuan Zeng, Rongxin Cai, Chunhou Li, Chuanbing Chen","doi":"10.1080/10826076.2023.2223640","DOIUrl":"https://doi.org/10.1080/10826076.2023.2223640","url":null,"abstract":"Abstract Zibushengfa Tablet is a widely consumed traditional Chinese medicine (TCM) prescription. However, its main bioactives remain unclear and its pharmacopeia quality-marker (Q-marker) is poorly characterized nowadays. The present study analyzed its lyophilized aqueous powder using ultra-high-performance liquid chromatography-quadrupole-orbitrap mass spectrometry. Through comparison with authentic standards in the library, 46 bioactives were putatively identified, including quinic acid, D-gluconic acid, gallic acid, protocatechuic acid, 5-caffeoylquinic acid, L-tryptophan, salidroside, (+)-catechin hydrate, caffeic acid, cryptochlorogenic acid, mangiferin, ferulic acid, myricetin-3-O-galactoside, 2,3,5,4’-tetrahydroxystilbene-2-O-glucoside, forsythoside B, myricitrin, rutin, hyperoside, acteoside, 3,4-dicaffeoylquinic acid, myricetin, senkyunolide A, isochlorogenic acid C, quercitrin, (+)-epipinoresinol, quercetin, biochanin A, calycosin, luteolin, wedelolactone, calycosin-7-O-glucoside, chrysoeriol, trans-cinnamic acid, formononetin, chrysin, 3,3’,4’,5,6,7,8-heptamethoxyflavone, Z-ligustilide, amentoflavone, tangeretin, hinokiflavone, emodin, physcion, linoleic acid, stearic acid, palmitic acid, and palmitic acid ethyl ester. Of these, four pairs of isomers were successfully differentiated using the newly established library-filtering strategy. On this basis, senkyunolide A, amentoflavone, quercetin, quercitrin, and wedelolactone are preliminarily suggested as additional quality-markers for the consideration by Pharmacopeia Committee, to prevent the possible adulterate. Graphical Abstract","PeriodicalId":16295,"journal":{"name":"Journal of Liquid Chromatography & Related Technologies","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41262260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Ginkgo biloba leaf extract (EGBL) is a valuable herbal extract. The main active ingredients are flavonoids, terpene lactones, organic acids and proanthocyanidins. In recent years, there have been many reports on the detection of the above active ingredients. In this paper, the analysis methods of chemical components in EGBL were reviewed, such as ultraviolet spectrophotometry, high-performance liquid chromatography, and quantitative nuclear magnetic resonance. The analysis methods of ginkgolic acids are also reviewed. Based on the current research progress, three suggestions are provided. Firstly, the quality marker research of EGBL needs to be strengthened. Secondly, more bioassay methods for EGBL quality control should be developed. Thirdly, it is necessary to strengthen the detection of proanthocyanidins in EGBL. GRAPHICAL ABSTRACT
{"title":"Advances on analysis methods of ginkgo biloba leaf extract","authors":"Jinhao Li, Jing Lan, Shufang Wang, Jianbiao Yao, Fangfang Wang, Xingchu Gong","doi":"10.1080/10826076.2023.2223618","DOIUrl":"https://doi.org/10.1080/10826076.2023.2223618","url":null,"abstract":"Abstract Ginkgo biloba leaf extract (EGBL) is a valuable herbal extract. The main active ingredients are flavonoids, terpene lactones, organic acids and proanthocyanidins. In recent years, there have been many reports on the detection of the above active ingredients. In this paper, the analysis methods of chemical components in EGBL were reviewed, such as ultraviolet spectrophotometry, high-performance liquid chromatography, and quantitative nuclear magnetic resonance. The analysis methods of ginkgolic acids are also reviewed. Based on the current research progress, three suggestions are provided. Firstly, the quality marker research of EGBL needs to be strengthened. Secondly, more bioassay methods for EGBL quality control should be developed. Thirdly, it is necessary to strengthen the detection of proanthocyanidins in EGBL. GRAPHICAL ABSTRACT","PeriodicalId":16295,"journal":{"name":"Journal of Liquid Chromatography & Related Technologies","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44537937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Glutathione (GSH) and cysteine are important antioxidants in maintaining the oxidation-reduction balance. 14,15-epoxyeicosatrienoic acid (14,15-EET) and 15-hydroxyeicosatetraenoic acid (15-HETE) are biological active compounds metabolized from arachidonic acid. To dynamically monitor GSH, cysteine, 14,15-EET, and 15-HETE, an ultra-high-performance liquid chromatography-mass spectrometry (UPLC-MS/MS) method was developed. The derivative GSH, cysteine, and underivatized 14,15-EET, 15-HETE were determined by electrospray ionization source with multiple reaction monitoring. The developed method was well validated and applied to monitor the extracellular concentration of GSH, cysteine, 14,15-EET, and 15-HETE in paraquat (PQ) poisoning LO2 cells. It was shown that the derivation of GSH and cysteine is complete and specific. There was a good linear relationship among GSH, cysteine, 14,15-EET, and 15-HETE. The relative standard deviations of precision and accuracy were all within 15%. After PQ poisoning, the extracellular concentrations of GSH and cysteine were increased at 72 h. 14,15-EET and 15-HETE started to increase at 24 h, earlier than GSH and cysteine. Irreversible pathological alterations appeared at 48 h. In conclusion, a fast and specific UPLC-MS/MS method for the determination of GSH, cysteine, 14,15-EET, and 15-HETE has been developed and validated. PQ induced the increase of 14,15-EET and 15-HETE earlier than that of GSH and cysteine in LO2 cells culture medium. Graphical Abstract
{"title":"Development of UPLC-MS/MS methods for quantitation of glutathione, cysteine, 14,15-epoxyeicosatrienoic acid, and 15-hydroxyeicosatetraenoic acid and application in paraquat poisoning cells","authors":"Cong-Rong Tang, Xingjie Zhu, Zheng Yu, Xiaofan Ke, Jianhui Yang, Zhongqiu Lu, Lufeng Hu","doi":"10.1080/10826076.2023.2223634","DOIUrl":"https://doi.org/10.1080/10826076.2023.2223634","url":null,"abstract":"Abstract Glutathione (GSH) and cysteine are important antioxidants in maintaining the oxidation-reduction balance. 14,15-epoxyeicosatrienoic acid (14,15-EET) and 15-hydroxyeicosatetraenoic acid (15-HETE) are biological active compounds metabolized from arachidonic acid. To dynamically monitor GSH, cysteine, 14,15-EET, and 15-HETE, an ultra-high-performance liquid chromatography-mass spectrometry (UPLC-MS/MS) method was developed. The derivative GSH, cysteine, and underivatized 14,15-EET, 15-HETE were determined by electrospray ionization source with multiple reaction monitoring. The developed method was well validated and applied to monitor the extracellular concentration of GSH, cysteine, 14,15-EET, and 15-HETE in paraquat (PQ) poisoning LO2 cells. It was shown that the derivation of GSH and cysteine is complete and specific. There was a good linear relationship among GSH, cysteine, 14,15-EET, and 15-HETE. The relative standard deviations of precision and accuracy were all within 15%. After PQ poisoning, the extracellular concentrations of GSH and cysteine were increased at 72 h. 14,15-EET and 15-HETE started to increase at 24 h, earlier than GSH and cysteine. Irreversible pathological alterations appeared at 48 h. In conclusion, a fast and specific UPLC-MS/MS method for the determination of GSH, cysteine, 14,15-EET, and 15-HETE has been developed and validated. PQ induced the increase of 14,15-EET and 15-HETE earlier than that of GSH and cysteine in LO2 cells culture medium. Graphical Abstract","PeriodicalId":16295,"journal":{"name":"Journal of Liquid Chromatography & Related Technologies","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48406216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-10DOI: 10.1080/10826076.2023.2222301
Mouna Necibi, N. Mzoughi
Abstract In the present work, we develop, validate, and compare two extraction procedures (in-tube liquid-liquid extraction (ITLLE) and solid-phase extraction (SPE)). The parameters that influence extraction efficiency, the nature and volume of the extraction solvent, as well as the pH nature, were improved and a standard solution of Organophosphorus pesticides (OPPs) was used. Results indicated that SPE is clearly more successful than ITLLE extraction under optimum conditions. The validated method showed that this approach had good linearity, with correlation coefficients ranging from 0.991 to 0.998 and low detection and quantification limits of 0.01–0.03 ng L−1 and 0.03–0.9 ng L−1, respectively. Relative standard deviations were in the range of 0.2 and 1.2%, while extraction recoveries varied from 79 to 97%. The SPE was selected and, optimum recoveries for OPPs were obtained at pH 7, with 10 mL of ethyl acetate. The suggested approach was effectively applied to the analysis of water samples collected from the Medjerda River in two seasons. The average OPPs concentrations ranged from not detected (ND) to 17.5 ng L−1 during the dry season and from not detected ND to 13.1 ng L−1 during the wet season. The results demonstrate that none of the OPPs concentrations measured exceeded the regulatory limits. Graphical Abstract
摘要在本工作中，我们开发、验证并比较了两种提取方法（管内液-液萃取（ITLLE）和固相萃取（SPE））。改进了影响萃取效率的参数、萃取溶剂的性质和体积以及pH性质，并使用了有机磷农药的标准溶液。结果表明，在最佳条件下，SPE的提取效果明显优于ITLLE。经验证的方法表明，该方法具有良好的线性，相关系数在0.991至0.998之间，检测和定量限在0.01至0.03之间 ng L−1和0.03–0.9 ng L−1。相对标准偏差在0.2%至1.2%之间，而提取回收率在79%至97%之间。选择SPE，并在pH为7时获得OPP的最佳回收率，10 mL乙酸乙酯。所提出的方法有效地应用于两个季节从Medjerda河采集的水样的分析。OPPs的平均浓度范围从未检测到（ND）到17.5 ng L−1在旱季，从未检测到的ND到13.1 ng L−1。结果表明，所测得的OPP浓度均未超过监管限值。图形摘要
{"title":"Comparative evaluation of solid-phase extraction and in-tube liquid-liquid extraction for the determination of Organophosphorus pesticides in the surface water from Medjerda River, Tunisia","authors":"Mouna Necibi, N. Mzoughi","doi":"10.1080/10826076.2023.2222301","DOIUrl":"https://doi.org/10.1080/10826076.2023.2222301","url":null,"abstract":"Abstract In the present work, we develop, validate, and compare two extraction procedures (in-tube liquid-liquid extraction (ITLLE) and solid-phase extraction (SPE)). The parameters that influence extraction efficiency, the nature and volume of the extraction solvent, as well as the pH nature, were improved and a standard solution of Organophosphorus pesticides (OPPs) was used. Results indicated that SPE is clearly more successful than ITLLE extraction under optimum conditions. The validated method showed that this approach had good linearity, with correlation coefficients ranging from 0.991 to 0.998 and low detection and quantification limits of 0.01–0.03 ng L−1 and 0.03–0.9 ng L−1, respectively. Relative standard deviations were in the range of 0.2 and 1.2%, while extraction recoveries varied from 79 to 97%. The SPE was selected and, optimum recoveries for OPPs were obtained at pH 7, with 10 mL of ethyl acetate. The suggested approach was effectively applied to the analysis of water samples collected from the Medjerda River in two seasons. The average OPPs concentrations ranged from not detected (ND) to 17.5 ng L−1 during the dry season and from not detected ND to 13.1 ng L−1 during the wet season. The results demonstrate that none of the OPPs concentrations measured exceeded the regulatory limits. Graphical Abstract","PeriodicalId":16295,"journal":{"name":"Journal of Liquid Chromatography & Related Technologies","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49503596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Qishan Formula (QSF) is mainly used to treat lung cancer and its side effects of radiotherapy and chemotherapy. Although the clinical efficacy of QSF has been recognized, its mechanism of action and active ingredients are still unclear. This study aims to explore the potential Q-Marker of QSF and reveal its anti-lung cancer mechanism. Firstly, the fingerprints of 10 batches of QSF were established, and the potential Q-markers were screened by chemometrics, then network pharmacology and molecular docking methods were used to explore its anti-lung cancer mechanism. Finally, the potential Q-markers were quantitatively by UPLC-QDA. The results showed that 48 common peaks were identified by the established fingerprint, and calycosin, ginsenoside Rg1, atractylenolide III, atractylenolide I and ginsenoside Rb1 were screened as potential Q-Markers of QSF by chemometrics. Network pharmacology and molecular docking found that the potential Q-Markers may regulate various signaling pathways through targets such as FOS, IL6, EGFR, ESR1, MAPK1, and MAPK3, and play a therapeutic role in lung cancer. Besides, the established UPLC-QDA can also perform quality control for Q-Markers of QSF. This study can provide academic ideas and lay a foundation for the comprehensive quality control and mechanism research of QSF and other compound preparation. Graphical Abstract
{"title":"Uncovering the Q-marker and potential mechanisms of Qishan Formula for lung cancer based on multi-technology","authors":"Yutong Sui, Yanqun Yang, Jing Zhang, Ziwei Wang, Xue Geng, Jiakang Jiang","doi":"10.1080/10826076.2023.2216765","DOIUrl":"https://doi.org/10.1080/10826076.2023.2216765","url":null,"abstract":"Abstract Qishan Formula (QSF) is mainly used to treat lung cancer and its side effects of radiotherapy and chemotherapy. Although the clinical efficacy of QSF has been recognized, its mechanism of action and active ingredients are still unclear. This study aims to explore the potential Q-Marker of QSF and reveal its anti-lung cancer mechanism. Firstly, the fingerprints of 10 batches of QSF were established, and the potential Q-markers were screened by chemometrics, then network pharmacology and molecular docking methods were used to explore its anti-lung cancer mechanism. Finally, the potential Q-markers were quantitatively by UPLC-QDA. The results showed that 48 common peaks were identified by the established fingerprint, and calycosin, ginsenoside Rg1, atractylenolide III, atractylenolide I and ginsenoside Rb1 were screened as potential Q-Markers of QSF by chemometrics. Network pharmacology and molecular docking found that the potential Q-Markers may regulate various signaling pathways through targets such as FOS, IL6, EGFR, ESR1, MAPK1, and MAPK3, and play a therapeutic role in lung cancer. Besides, the established UPLC-QDA can also perform quality control for Q-Markers of QSF. This study can provide academic ideas and lay a foundation for the comprehensive quality control and mechanism research of QSF and other compound preparation. Graphical Abstract","PeriodicalId":16295,"journal":{"name":"Journal of Liquid Chromatography & Related Technologies","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47454031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-16DOI: 10.1080/10826076.2023.2206667
Man-Jiang Xie, Jie Wang, Chenguang Dong, P. Tu, Qingying Zhang
Abstract Based on HPLC fingerprint analysis and multi-components quantification, a simple, sensible, and reliable HPLC-UV method, capable of qualitatively and quantitatively characterizing the main chemical markers of all medicinal herbs of Zaoren Anshen (ZRAS) prescription by one injection, was firstly developed. Then, three different dosage forms of ZRAS preparations, i.e., capsule, granule, and oral liquid, were analyzed and compared systematically, indicating the similarity and difference in chemical markers of the three ZRAS preparations. The strategy of combining HPLC chemical fingerprint and multi-components quantification in one injection was rather practical, powerful and useful in characterizing the main chemical markers of ZRAS preparations, which was a benefit for holistic quality evaluation and control of ZRAS preparations. Moreover, the present results provide sound scientific data for further studies on the effective constituents and quality control of ZRAS preparations. GRAPHICAL ABSTRACT
{"title":"Holistic chemical characterization of Zaoren Anshen preparations based on HPLC fingerprint and multi-components quantification","authors":"Man-Jiang Xie, Jie Wang, Chenguang Dong, P. Tu, Qingying Zhang","doi":"10.1080/10826076.2023.2206667","DOIUrl":"https://doi.org/10.1080/10826076.2023.2206667","url":null,"abstract":"Abstract Based on HPLC fingerprint analysis and multi-components quantification, a simple, sensible, and reliable HPLC-UV method, capable of qualitatively and quantitatively characterizing the main chemical markers of all medicinal herbs of Zaoren Anshen (ZRAS) prescription by one injection, was firstly developed. Then, three different dosage forms of ZRAS preparations, i.e., capsule, granule, and oral liquid, were analyzed and compared systematically, indicating the similarity and difference in chemical markers of the three ZRAS preparations. The strategy of combining HPLC chemical fingerprint and multi-components quantification in one injection was rather practical, powerful and useful in characterizing the main chemical markers of ZRAS preparations, which was a benefit for holistic quality evaluation and control of ZRAS preparations. Moreover, the present results provide sound scientific data for further studies on the effective constituents and quality control of ZRAS preparations. GRAPHICAL ABSTRACT","PeriodicalId":16295,"journal":{"name":"Journal of Liquid Chromatography & Related Technologies","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45300449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-16DOI: 10.1080/10826076.2023.2202238
Lujie Lu, Wen-xin Wang, Peijun Sun, Shuwei Yan, Hui Chen, Xiao Liu, Jiajia Dong, Lihong Chen, T. Lu
Abstract Licorice is mainly used to treat cough in clinics. After being roasted with honey, licorice has a cardiac protective effect. Due to its different sources, the chemical components in licorice are also diverse. To distinguish the different compounds between three sources of raw licorice (RL) and between three sources of honey-roasted licorice (HRL), a UPLC-QTOF-MS/MS method was established. Eighteen batches of samples were analyzed which were derived from Glycyrrhiza uralensis Fisch., Glycyrrhiza inflata Bat., and Glycyrrhiza glabra L. In addition, the potential key pathways of HRL to anti-arrhythmia were explored by network pharmacology. Among the three sources of RL, 77 differential compounds were detected in the positive ion mode; and 62 differential compounds were detected in the negative ion mode, of which 21 differential compounds were detected in both ion modes. Twenty-nine differential compounds were identified before and after honey-roasted of G. uralensis Fisch., 51 and 17 differential compounds were identified in G. glabra L. and G. inflata Bat., receptively. The network pharmacological analysis predicted that Rap1, Ras, PI3K-Akt, and cAMP signaling pathway might be closely related to the anti-arrhythmia mechanism of HRL. This study provides a theoretical reference for the quality improvement and clinical application of licorice. Graphical Abstract
{"title":"Differential compounds of licorice before and after honey roasted and anti-arrhythmia mechanism via LC-MS/MS and network pharmacology analysis","authors":"Lujie Lu, Wen-xin Wang, Peijun Sun, Shuwei Yan, Hui Chen, Xiao Liu, Jiajia Dong, Lihong Chen, T. Lu","doi":"10.1080/10826076.2023.2202238","DOIUrl":"https://doi.org/10.1080/10826076.2023.2202238","url":null,"abstract":"Abstract Licorice is mainly used to treat cough in clinics. After being roasted with honey, licorice has a cardiac protective effect. Due to its different sources, the chemical components in licorice are also diverse. To distinguish the different compounds between three sources of raw licorice (RL) and between three sources of honey-roasted licorice (HRL), a UPLC-QTOF-MS/MS method was established. Eighteen batches of samples were analyzed which were derived from Glycyrrhiza uralensis Fisch., Glycyrrhiza inflata Bat., and Glycyrrhiza glabra L. In addition, the potential key pathways of HRL to anti-arrhythmia were explored by network pharmacology. Among the three sources of RL, 77 differential compounds were detected in the positive ion mode; and 62 differential compounds were detected in the negative ion mode, of which 21 differential compounds were detected in both ion modes. Twenty-nine differential compounds were identified before and after honey-roasted of G. uralensis Fisch., 51 and 17 differential compounds were identified in G. glabra L. and G. inflata Bat., receptively. The network pharmacological analysis predicted that Rap1, Ras, PI3K-Akt, and cAMP signaling pathway might be closely related to the anti-arrhythmia mechanism of HRL. This study provides a theoretical reference for the quality improvement and clinical application of licorice. Graphical Abstract","PeriodicalId":16295,"journal":{"name":"Journal of Liquid Chromatography & Related Technologies","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47155385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-16DOI: 10.1080/10826076.2023.2204238
Aggarapu Susmitha, G. Rajitha, Gireesh Kumar Eri
Abstract Quality has been a prime priority for the pharmaceutical sector with the development of recommendations by regulatory organizations like ICH. Concepts of ICH Q8 to Q10 are the foundation of the QbD methodology, a systematic approach to pharmaceutical development. Pharma industries have adopted the QbD approach to support product quality during manufacturing and to improve robust manufacturing processes. QbD adoption in analytical procedure development is AQbD, Analytical Quality by Design. It aids in reliable, economical analytical procedure development and also facilitates regulatory flexibility. This review illustrates the detailed aspects with relevant examples in each step of AQbD and DoE techniques applied to various analytical techniques for analytical procedure development for the determination of medicinal compounds. The overview of techniques created using AQbD contributes to the direction in this field of study. Visual Abstract
{"title":"A comprehensive review on QbD driven analytical procedures developed for the analysis of various drugs","authors":"Aggarapu Susmitha, G. Rajitha, Gireesh Kumar Eri","doi":"10.1080/10826076.2023.2204238","DOIUrl":"https://doi.org/10.1080/10826076.2023.2204238","url":null,"abstract":"Abstract Quality has been a prime priority for the pharmaceutical sector with the development of recommendations by regulatory organizations like ICH. Concepts of ICH Q8 to Q10 are the foundation of the QbD methodology, a systematic approach to pharmaceutical development. Pharma industries have adopted the QbD approach to support product quality during manufacturing and to improve robust manufacturing processes. QbD adoption in analytical procedure development is AQbD, Analytical Quality by Design. It aids in reliable, economical analytical procedure development and also facilitates regulatory flexibility. This review illustrates the detailed aspects with relevant examples in each step of AQbD and DoE techniques applied to various analytical techniques for analytical procedure development for the determination of medicinal compounds. The overview of techniques created using AQbD contributes to the direction in this field of study. Visual Abstract","PeriodicalId":16295,"journal":{"name":"Journal of Liquid Chromatography & Related Technologies","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41260918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-16DOI: 10.1080/10826076.2023.2208201
Mengdi Yuan, Chenglin Zhong, K. Row, Guizhen Li
Abstract Hydrophilic molecularly imprinted chitosan microspheres (HMICS) were synthesized using deep eutectic solvents (DESs)-(3-aminopropyl) triethoxysilane (APTES) as co-functional monomer for the extraction of catechin from green tea with micro-solid phase extraction (micro-SPE). Various factors were studied to optimize the extraction condition during extraction process. The actual extraction amount under the optimal conditions (amount of adsorbent (20 mg), type and volume of washing (acetone, 1.2 mL) and eluting solvent (acetone/acetic acid, 1.0 mL), extraction time (30 min), extraction temperature (40 °C), and sample pH (4)) were obtained at 97.06 mg·g−1. The catechin recovery ranged with ranged with limit of detection (LOD) within 0.09–0.24 µg·mL−1, and limit of quantification (LOQ) within 0.18–0.28 µg·mL−1. From the experimental results in this system, the HMICS was proved to have higher extraction capacity, improving as a promising adsorbent for the extraction of catechin. GRAPHICAL ABSTRACT
{"title":"Hydrophilic molecularly imprinted chitosan microspheres based on deep eutectic solvents for micro-solid phase extraction of catechin","authors":"Mengdi Yuan, Chenglin Zhong, K. Row, Guizhen Li","doi":"10.1080/10826076.2023.2208201","DOIUrl":"https://doi.org/10.1080/10826076.2023.2208201","url":null,"abstract":"Abstract Hydrophilic molecularly imprinted chitosan microspheres (HMICS) were synthesized using deep eutectic solvents (DESs)-(3-aminopropyl) triethoxysilane (APTES) as co-functional monomer for the extraction of catechin from green tea with micro-solid phase extraction (micro-SPE). Various factors were studied to optimize the extraction condition during extraction process. The actual extraction amount under the optimal conditions (amount of adsorbent (20 mg), type and volume of washing (acetone, 1.2 mL) and eluting solvent (acetone/acetic acid, 1.0 mL), extraction time (30 min), extraction temperature (40 °C), and sample pH (4)) were obtained at 97.06 mg·g−1. The catechin recovery ranged with ranged with limit of detection (LOD) within 0.09–0.24 µg·mL−1, and limit of quantification (LOQ) within 0.18–0.28 µg·mL−1. From the experimental results in this system, the HMICS was proved to have higher extraction capacity, improving as a promising adsorbent for the extraction of catechin. GRAPHICAL ABSTRACT","PeriodicalId":16295,"journal":{"name":"Journal of Liquid Chromatography & Related Technologies","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41786708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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