Pub Date : 2024-05-03DOI: 10.21608/djvs.2023.242255.1124
Besheer G. Elshafey, Rania Mayel, Mohamed Nasr, Noha Beder, Nagwa I El-Kasrawy, Asmaa Saleh
: Seven hundred-eighty dairy cows in Kafr-Elshikh and El-Beheira provinces were tested for subclinical ketosis (SCK) using qualitative determination of β -hydroxybutyric acid (BHBA) in urine from January to December 2022. All 280 positive cows were gathered in a questionnaire. The clinical examination, serum biochemical analysis, complete blood picture, and liver ultrasound of 89 subclinical ketotic cows were all performed. Our findings showed that both ruminal motility and milk production had significantly decreased while serum levels of BHBA, non-esterified fatty acid (NEFA), TAG, GGT, AST, ALT significantly elevated, blood glucose, total protein, albumin, and calcium levels decreased. Hb, RBCs, PCV, MCV, MCH, and MCHC, whereas WBCs, neutrophils, eosinophils, basophils, monocytes, and neutrophil-lymphocyte (N/L) ratio significantly increased. These cow's ultrasonography examinations revealed the typical signs of fatty liver. The prevalence of subclinical ketosis was 35.9 %. The development of SCK in dairy cows is influenced by various risk factors such as cows at the first 60 days of lactation, body condition score (3.5-5), age greater than 6 years, high milk production, and parity greater than three times. Early detection of SCK in early postpartum dairy cows is crucial to prevent financial loss to the farmers. Routine estimation of SCK in early postpartum dairy cows can be achieved using BHBA and NEFA estimation, along with hepatic ultrasonography.
{"title":"Hemato-biochemical and Ultrasonographic Studies on Subclinical Ketosis in Dairy Cows","authors":"Besheer G. Elshafey, Rania Mayel, Mohamed Nasr, Noha Beder, Nagwa I El-Kasrawy, Asmaa Saleh","doi":"10.21608/djvs.2023.242255.1124","DOIUrl":"https://doi.org/10.21608/djvs.2023.242255.1124","url":null,"abstract":": Seven hundred-eighty dairy cows in Kafr-Elshikh and El-Beheira provinces were tested for subclinical ketosis (SCK) using qualitative determination of β -hydroxybutyric acid (BHBA) in urine from January to December 2022. All 280 positive cows were gathered in a questionnaire. The clinical examination, serum biochemical analysis, complete blood picture, and liver ultrasound of 89 subclinical ketotic cows were all performed. Our findings showed that both ruminal motility and milk production had significantly decreased while serum levels of BHBA, non-esterified fatty acid (NEFA), TAG, GGT, AST, ALT significantly elevated, blood glucose, total protein, albumin, and calcium levels decreased. Hb, RBCs, PCV, MCV, MCH, and MCHC, whereas WBCs, neutrophils, eosinophils, basophils, monocytes, and neutrophil-lymphocyte (N/L) ratio significantly increased. These cow's ultrasonography examinations revealed the typical signs of fatty liver. The prevalence of subclinical ketosis was 35.9 %. The development of SCK in dairy cows is influenced by various risk factors such as cows at the first 60 days of lactation, body condition score (3.5-5), age greater than 6 years, high milk production, and parity greater than three times. Early detection of SCK in early postpartum dairy cows is crucial to prevent financial loss to the farmers. Routine estimation of SCK in early postpartum dairy cows can be achieved using BHBA and NEFA estimation, along with hepatic ultrasonography.","PeriodicalId":166243,"journal":{"name":"Damanhour Journal of Veterinary Sciences","volume":"39 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141017092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-06DOI: 10.21608/djvs.2023.208559.1115
A. Badran, Mousa A. Ayoub, Nabil Bakear, Sabah I Shaaban
: The current work was carried out in El-Beheira Province, Egypt to estimate the frequency of Candida species occurrence in mastitic milk of cattle and buffalo as well as oral swabs from humans besides detection of some virulence factors and determine the antifungal susceptibility of the obtained Candida strains . A total of 350 raw milk samples were randomly collected from clinically mastitic bovine milk. Also, 140 oral swabs were obtained from patients attending external clinics of El-Delingat Hospital to be examined for the presence of Candida. The recorded results showed a high frequency of Candida spp. (36.9%) among the examined milk samples. Candida spp. isolated from cow milk (39.9%) was higher than that isolated from buffalo milk (29.3%). Also, the total prevalence of Candidiasis in human samples was 62.9% and the highest prevalence in relation to age groups was noticed in the age group > 2 - < 16 years (65.5%) and finally, the age group >40 years (58.8%). The identified Candida spp. in human samples were C. albicans (37.5%), C. glabrata (17.1%) , C. krusei (14.8%), C. parapsilosis (13.6%), C. tropicalis (11.4%), C. guilliermondii (4.5%) and C. kefyr (1.1%). It was observed that the highest prevalence of Candida species was among artificially fed infants (77.8%) and the lowest prevalence was detected in breastfed infants (53.3%). Based on the recorded results, Candida species constitute a major threat to both dairy animals and humans. Also, improperly heated milk constitutes a potential source of infection for humans so efficient pasteurization must be performed to avoid this risk.
{"title":"Candidiasis in Mastitic Milk and Humans in El-Beheira Province, Egypt: Isolation, Molecular Characterization, and Antifungal Susceptibility","authors":"A. Badran, Mousa A. Ayoub, Nabil Bakear, Sabah I Shaaban","doi":"10.21608/djvs.2023.208559.1115","DOIUrl":"https://doi.org/10.21608/djvs.2023.208559.1115","url":null,"abstract":": The current work was carried out in El-Beheira Province, Egypt to estimate the frequency of Candida species occurrence in mastitic milk of cattle and buffalo as well as oral swabs from humans besides detection of some virulence factors and determine the antifungal susceptibility of the obtained Candida strains . A total of 350 raw milk samples were randomly collected from clinically mastitic bovine milk. Also, 140 oral swabs were obtained from patients attending external clinics of El-Delingat Hospital to be examined for the presence of Candida. The recorded results showed a high frequency of Candida spp. (36.9%) among the examined milk samples. Candida spp. isolated from cow milk (39.9%) was higher than that isolated from buffalo milk (29.3%). Also, the total prevalence of Candidiasis in human samples was 62.9% and the highest prevalence in relation to age groups was noticed in the age group > 2 - < 16 years (65.5%) and finally, the age group >40 years (58.8%). The identified Candida spp. in human samples were C. albicans (37.5%), C. glabrata (17.1%) , C. krusei (14.8%), C. parapsilosis (13.6%), C. tropicalis (11.4%), C. guilliermondii (4.5%) and C. kefyr (1.1%). It was observed that the highest prevalence of Candida species was among artificially fed infants (77.8%) and the lowest prevalence was detected in breastfed infants (53.3%). Based on the recorded results, Candida species constitute a major threat to both dairy animals and humans. Also, improperly heated milk constitutes a potential source of infection for humans so efficient pasteurization must be performed to avoid this risk.","PeriodicalId":166243,"journal":{"name":"Damanhour Journal of Veterinary Sciences","volume":"8 13","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140397194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-11DOI: 10.21608/djvs.2024.266721.1129
M. Elbehiry, Ayman Abd El-Aziz, Mohamed Belal, K. Sobhy
: Egg yolk is included in semen extenders because it prevents cold shock during chilling. This study aimed to assess the impact of substituting egg yolk with low-density lipoprotein (LDL) in Tris-Citric-Fructose (TCF) chilled extenders, with or without ascorbic acid, on the canine semen quality at 24, 48, and 72 hours of cooling. Semen from three German shepherd dogs was evaluated and extended at a 1:1 ratio using four extenders: Group A (TCF buffer, 20% egg yolk, with ascorbic acid), Group B (TCF buffer, 20% egg yolk without ascorbic acid), Group C (TCF buffer, 20% LDL, with ascorbic acid), and Group D (TCF buffer, 20% LDL without ascorbic acid). The extended semen was stored at 4°C and assessed after 24, 48, and 72 hours of cooling. The egg yolk extender resulted in less reduction in progressive sperm motility after 24, 48, and 72 hours of cooling; however, reduction in normal morphology, acrosome integrity, and cell membrane integrity were lower in TCF-LDL chilled extender than in TCF-EY chilled extender. Furthermore, after 24 hours of cooling, all evaluation parameters in both extenders were greater than 50%. In conclusion, the quality of chilled canine sperm was significantly improved by adding ascorbic acid to TCF-EY, but the improvement was only marginal when applied to TCF-LDL chilled extender.
{"title":"The Impact of Using Extenders Containing Low-density Lipoprotein Versus Egg Yolk with or without Ascorbic Acid on the Fertility Parameters of Post-thawed Chilled Canine Semen","authors":"M. Elbehiry, Ayman Abd El-Aziz, Mohamed Belal, K. Sobhy","doi":"10.21608/djvs.2024.266721.1129","DOIUrl":"https://doi.org/10.21608/djvs.2024.266721.1129","url":null,"abstract":": Egg yolk is included in semen extenders because it prevents cold shock during chilling. This study aimed to assess the impact of substituting egg yolk with low-density lipoprotein (LDL) in Tris-Citric-Fructose (TCF) chilled extenders, with or without ascorbic acid, on the canine semen quality at 24, 48, and 72 hours of cooling. Semen from three German shepherd dogs was evaluated and extended at a 1:1 ratio using four extenders: Group A (TCF buffer, 20% egg yolk, with ascorbic acid), Group B (TCF buffer, 20% egg yolk without ascorbic acid), Group C (TCF buffer, 20% LDL, with ascorbic acid), and Group D (TCF buffer, 20% LDL without ascorbic acid). The extended semen was stored at 4°C and assessed after 24, 48, and 72 hours of cooling. The egg yolk extender resulted in less reduction in progressive sperm motility after 24, 48, and 72 hours of cooling; however, reduction in normal morphology, acrosome integrity, and cell membrane integrity were lower in TCF-LDL chilled extender than in TCF-EY chilled extender. Furthermore, after 24 hours of cooling, all evaluation parameters in both extenders were greater than 50%. In conclusion, the quality of chilled canine sperm was significantly improved by adding ascorbic acid to TCF-EY, but the improvement was only marginal when applied to TCF-LDL chilled extender.","PeriodicalId":166243,"journal":{"name":"Damanhour Journal of Veterinary Sciences","volume":"9 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140458966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-17DOI: 10.21608/djvs.2023.248877.1125
Rehab R. Abd El Maged, Nesma Rasheed, Iman Ibrahim, Hebatallah Saad, G. Batiha, Attia A. Abou Zaid
Fish are frequently regarded as the most significant source of income due to their high economic value. The infection of monogeneans is the main cause of fish anemia leading to high mortalities. Thus, this study aimed to detect monogeneans parasites in Scoberomorus commerson and investigate the histopathological changes related to its natural infections. Furthermore, to research the potential parasite-host interactions. One hundred marine fishes ( S. commerson ) from the Ezbet-El Borg area, Damietta province, Egypt were examined from August 2022 to January 2023 for the presence of monogenean gill parasites. 38 fish were infected with monogenean species: Pricea multae 32% and Gotocotyla acanthura 19%. The structure of P. multae and G. acanthura were explained by using light and scanning electron microscope (SEM), the infected fish have no pathognomonic lesions, but for a few cases showed excessive mucus secretion. The pathological and ultrastructural results revealed massive branchial damage with partial to complete lamellar fusion and lamellar desquamation with complete loss of secondary lamellae and lymphocytic infiltration, the host response includes the appearance of numerous ruffled lymphocytes and mucous globules at the site of parasitic attachment.
{"title":"New Insights on the Effects of Monogenean Gill Parasites on Naturally Infested Scoberomorus commerson: Host Response, Electron Microscopy, and Histopathological Studies","authors":"Rehab R. Abd El Maged, Nesma Rasheed, Iman Ibrahim, Hebatallah Saad, G. Batiha, Attia A. Abou Zaid","doi":"10.21608/djvs.2023.248877.1125","DOIUrl":"https://doi.org/10.21608/djvs.2023.248877.1125","url":null,"abstract":"Fish are frequently regarded as the most significant source of income due to their high economic value. The infection of monogeneans is the main cause of fish anemia leading to high mortalities. Thus, this study aimed to detect monogeneans parasites in Scoberomorus commerson and investigate the histopathological changes related to its natural infections. Furthermore, to research the potential parasite-host interactions. One hundred marine fishes ( S. commerson ) from the Ezbet-El Borg area, Damietta province, Egypt were examined from August 2022 to January 2023 for the presence of monogenean gill parasites. 38 fish were infected with monogenean species: Pricea multae 32% and Gotocotyla acanthura 19%. The structure of P. multae and G. acanthura were explained by using light and scanning electron microscope (SEM), the infected fish have no pathognomonic lesions, but for a few cases showed excessive mucus secretion. The pathological and ultrastructural results revealed massive branchial damage with partial to complete lamellar fusion and lamellar desquamation with complete loss of secondary lamellae and lymphocytic infiltration, the host response includes the appearance of numerous ruffled lymphocytes and mucous globules at the site of parasitic attachment.","PeriodicalId":166243,"journal":{"name":"Damanhour Journal of Veterinary Sciences","volume":" 957","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139617667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-16DOI: 10.21608/djvs.2023.238314.1121
Mostafa Mahboob, M. Elbehiry
This study aimed to characterize the postpartum (pp) follicular development and the process of resumption of ovarian activity in dairy cows. Six multiparous lactating Holstein cows were kept and examined twice weekly using ultrasound from day 11-19 PP until the appearance of a large mature corpus luteum (CL). The results showed that four of six cows (66.66%) exhibited dominant follicles (DF >10 mm) within 2 weeks PP (day 11, 15, 15, 16) and one cow (16.6%) needed 3 weeks (day 19) to develop DF. While only one cow (16.6%) displayed a delay (30 days pp) in the appearance of DF. The shortest interval between calving to the beginning of 1st luteal formation was 15 days recorded for one cow and the longest interval was 57 days and recorded also for one cow. While, in the other 3 cows, 29, 33, 43 days were recorded as the interval from calving to formation of first luteal tissue. The development of cysts following anovulated follicles with the size of 19-20mm occurred in two cows (33.3%). The persistence of cysts was longer in larger size cysts compared with the smaller one. Spontaneous recovery of the smaller cyst was faster than the larger cyst (10 vs. 23 days). Altogether, results showed that of the ultrasound scanning can be used as a guide to follow up the ovarian activity during PP period and thereby, identifying the optimal therapeutical and managemental interference to solve any reproductive disorder and maintain standard fertility parameters within a dairy herd.
{"title":"Time Lapse Changes in the Ovarian Activity of Dairy Cows During the Postpartum Period Guided by Ultrasonography: A Pilot Study","authors":"Mostafa Mahboob, M. Elbehiry","doi":"10.21608/djvs.2023.238314.1121","DOIUrl":"https://doi.org/10.21608/djvs.2023.238314.1121","url":null,"abstract":"This study aimed to characterize the postpartum (pp) follicular development and the process of resumption of ovarian activity in dairy cows. Six multiparous lactating Holstein cows were kept and examined twice weekly using ultrasound from day 11-19 PP until the appearance of a large mature corpus luteum (CL). The results showed that four of six cows (66.66%) exhibited dominant follicles (DF >10 mm) within 2 weeks PP (day 11, 15, 15, 16) and one cow (16.6%) needed 3 weeks (day 19) to develop DF. While only one cow (16.6%) displayed a delay (30 days pp) in the appearance of DF. The shortest interval between calving to the beginning of 1st luteal formation was 15 days recorded for one cow and the longest interval was 57 days and recorded also for one cow. While, in the other 3 cows, 29, 33, 43 days were recorded as the interval from calving to formation of first luteal tissue. The development of cysts following anovulated follicles with the size of 19-20mm occurred in two cows (33.3%). The persistence of cysts was longer in larger size cysts compared with the smaller one. Spontaneous recovery of the smaller cyst was faster than the larger cyst (10 vs. 23 days). Altogether, results showed that of the ultrasound scanning can be used as a guide to follow up the ovarian activity during PP period and thereby, identifying the optimal therapeutical and managemental interference to solve any reproductive disorder and maintain standard fertility parameters within a dairy herd.","PeriodicalId":166243,"journal":{"name":"Damanhour Journal of Veterinary Sciences","volume":" 17","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139618997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-01DOI: 10.21608/djvs.2023.207832.1114
M. Elbehiry
: Infertility caused by smooth inactive ovaries (SIO) is a common ovarian disorder that causes a major reproductive failure in dairy cows and has a significant economic impact on the dairy industry. The present study aimed to investigate the response of dairy cows with a syndrome of smooth inactive ovaries to different hormonal treatment protocols and their impact on reproductive performances. Lactating multiparous Holstein cows ( n = 75) having smooth inactive ovaries diagnosed by two successive ultrasonographic examinations performed at 10 days interval were used in this study. Cows were assigned randomly into 5 groups (15 each). Group A, treated with a single injection of 250 µg GnRH, Group B with a single injection of 2500 IU HCG, Group C, in which cows received CIDR for 7 days and PGF2α at day 6 (24 hours before removal of CIDR followed by injection of 250 µg GnRH (at day 8) 24 hours after removal of CIDR (at day 8)(CIDR, PGF2α and GnRH), Group D in which cows received CIDR for 7 days and PGF2α at day 6 (24 hours before removal of CIDR) followed by injection of 2500 IU hCG 24 hours after removal of CIDR (CIDR, PGf2α, and HCG), and Group E in which cows did not receive any treatment and kept as a control group. The results showed that CIDR+ GnRH and CIDR+ hCG protocols achieved the full (100%) estrus induction rate (EIR). hCG group achieved 93% and GnRH group 80%, while the control group only achieved 20% EIR. The shortest treatment estrus intervals (TEIs) were recorded in CIDR+ hCG and CIDR+ GnRH protocols. Mean±SD of TEIs were 1 ± 0, 1.4 ± 1.1days 10.4 ± 5.4, 16.7 ±7.1 and 23.7± 2.9 days in CIDR+ hCG . CIDR+ GnRH, HCG, GnRH and control groups respectively. The CIDR+ hCG group had the highest pregnancy rate (86.7%), followed by hCG (79.9%) and CIDR+ GnRH groups (80%), and GnRH group with 73%. The control group showed a 13.3 % pregnancy rate however, GnRH group showed the highest total conception rate (91.6%). It was concluded that the highest EIR and shortest TEI were obtained in CIDR+ GnRH and CIDR+ hCG protocols. While the CIDR+ hCG group had the highest pregnancy rate (86.7%).
{"title":"Impact of different hormonal protocols for the treatment of smooth inactive ovaries (SIO) and the subsequent reproductive performance in dairy cows","authors":"M. Elbehiry","doi":"10.21608/djvs.2023.207832.1114","DOIUrl":"https://doi.org/10.21608/djvs.2023.207832.1114","url":null,"abstract":": Infertility caused by smooth inactive ovaries (SIO) is a common ovarian disorder that causes a major reproductive failure in dairy cows and has a significant economic impact on the dairy industry. The present study aimed to investigate the response of dairy cows with a syndrome of smooth inactive ovaries to different hormonal treatment protocols and their impact on reproductive performances. Lactating multiparous Holstein cows ( n = 75) having smooth inactive ovaries diagnosed by two successive ultrasonographic examinations performed at 10 days interval were used in this study. Cows were assigned randomly into 5 groups (15 each). Group A, treated with a single injection of 250 µg GnRH, Group B with a single injection of 2500 IU HCG, Group C, in which cows received CIDR for 7 days and PGF2α at day 6 (24 hours before removal of CIDR followed by injection of 250 µg GnRH (at day 8) 24 hours after removal of CIDR (at day 8)(CIDR, PGF2α and GnRH), Group D in which cows received CIDR for 7 days and PGF2α at day 6 (24 hours before removal of CIDR) followed by injection of 2500 IU hCG 24 hours after removal of CIDR (CIDR, PGf2α, and HCG), and Group E in which cows did not receive any treatment and kept as a control group. The results showed that CIDR+ GnRH and CIDR+ hCG protocols achieved the full (100%) estrus induction rate (EIR). hCG group achieved 93% and GnRH group 80%, while the control group only achieved 20% EIR. The shortest treatment estrus intervals (TEIs) were recorded in CIDR+ hCG and CIDR+ GnRH protocols. Mean±SD of TEIs were 1 ± 0, 1.4 ± 1.1days 10.4 ± 5.4, 16.7 ±7.1 and 23.7± 2.9 days in CIDR+ hCG . CIDR+ GnRH, HCG, GnRH and control groups respectively. The CIDR+ hCG group had the highest pregnancy rate (86.7%), followed by hCG (79.9%) and CIDR+ GnRH groups (80%), and GnRH group with 73%. The control group showed a 13.3 % pregnancy rate however, GnRH group showed the highest total conception rate (91.6%). It was concluded that the highest EIR and shortest TEI were obtained in CIDR+ GnRH and CIDR+ hCG protocols. While the CIDR+ hCG group had the highest pregnancy rate (86.7%).","PeriodicalId":166243,"journal":{"name":"Damanhour Journal of Veterinary Sciences","volume":" 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138618224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-17DOI: 10.21608/djvs.2023.238564.1122
Eman Younis, Marwa Hassan, Pierre Mehanny, Asmaa Abd El-Monsef, Eman Laz, M. Mansour
: The aim of the current study was to evaluate the possible protective influences of Nano selenium (Nano Se), probiotics and vitamin C against ochratoxin-A (OTA) induced hepatorenal injury and oxidative stress in male rats. Forty-five albino rats were divided into 9 equal groups. G1: Control, G2: Ochra 50 µg/kg b.wt, G3: Ochra + Nano Se 0.31 mg/kg b.wt, G4: Ochra+ vitamin C 1000 mg /L water, G5: Ochra+ Probiotic 400 g/ton of diet, G6: Ochra + Nano Se + vitamin C, G7: Ochra + Nano Se + Probiotic, G8: Ochra + Nano Se + vitamin C+ Probiotic, G9: Ochra + vitamin C + Probiotic. The obtained results showed significant increase in serum AST, GGT, ALT, and LDH activities, Creatinine, urea, TNF-α , IL-6, SOD, CAT, MDA, alpha 1, gamma 2, T. alpha and T4 concentrations in OTA intoxicated rats. Meanwhile, a significant decrease in GSH, TAC, total protein and its fractions including (albumin, Total beta, Total gamma, Total globulin, alpha 2, beta 1, gamma 1), albumin/globulin (A/G) ratio in addition to serum testosterone, estradiol, TSH and T3 concentrations were observed in OTA exposed rats. The results suggested that NanoSe, probiotics, and vitamin C are strong antioxidants agents that might ameliorate OTA-induced renal and liver damage in rats by combating free radical and attenuate oxidative stress. Also, Nanoselenium, probiotics, and vitamin C, were found to be effective in protecting rats against OTA-induced hepato-renal injury by anti-inflammatory mechanism with the best result of Nano selenium or probiotic alone and their combination.
:本研究旨在评估纳米硒（Nano Se）、益生菌和维生素 C 对赭曲霉毒素-A（OTA）诱导的雄性大鼠肝肾损伤和氧化应激可能产生的保护性影响。45 只白化大鼠被分为 9 个等量组。G1: 对照组，G2：G2：Ochra 50 µg/kg b.wt；G3：Ochra + Nano Se 0.31 mg/kg b.wt；G4：Ochra + 维生素 C 1000 mg /L 水；G5：Ochra + 益生菌 400 g/ton diet；G6：Ochra + Nano Se + 维生素 C；G7：Ochra + Nano Se + 益生菌；G8：Ochra + Nano Se + 维生素 C + 益生菌；G9：Ochra + 维生素 C + 益生菌。结果表明，OTA 中毒大鼠的血清 AST、GGT、ALT 和 LDH 活性、肌酐、尿素、TNF-α、IL-6、SOD、CAT、MDA、α1、γ2、T.同时，在暴露于 OTA 的大鼠体内，除了血清睾酮、雌二醇、促甲状腺激素和 T3 的浓度外，还观察到 GSH、TAC、总蛋白及其组分（白蛋白、总 beta、总 gamma、总球蛋白、α 2、β 1、γ 1）、白蛋白/球蛋白（A/G）比值明显下降。结果表明，纳米硒、益生菌和维生素 C 都是强抗氧化剂，可通过对抗自由基和减轻氧化应激来改善 OTA 引起的大鼠肾脏和肝脏损伤。研究还发现，纳米硒、益生菌和维生素 C 能通过抗炎机制有效地保护大鼠免受 OTA 引起的肝肾损伤，其中纳米硒或益生菌单独使用以及两者结合使用的效果最佳。
{"title":"The Effective Role of Nano Selenium and Probiotic in Reducing Ochratoxicosis in Rats","authors":"Eman Younis, Marwa Hassan, Pierre Mehanny, Asmaa Abd El-Monsef, Eman Laz, M. Mansour","doi":"10.21608/djvs.2023.238564.1122","DOIUrl":"https://doi.org/10.21608/djvs.2023.238564.1122","url":null,"abstract":": The aim of the current study was to evaluate the possible protective influences of Nano selenium (Nano Se), probiotics and vitamin C against ochratoxin-A (OTA) induced hepatorenal injury and oxidative stress in male rats. Forty-five albino rats were divided into 9 equal groups. G1: Control, G2: Ochra 50 µg/kg b.wt, G3: Ochra + Nano Se 0.31 mg/kg b.wt, G4: Ochra+ vitamin C 1000 mg /L water, G5: Ochra+ Probiotic 400 g/ton of diet, G6: Ochra + Nano Se + vitamin C, G7: Ochra + Nano Se + Probiotic, G8: Ochra + Nano Se + vitamin C+ Probiotic, G9: Ochra + vitamin C + Probiotic. The obtained results showed significant increase in serum AST, GGT, ALT, and LDH activities, Creatinine, urea, TNF-α , IL-6, SOD, CAT, MDA, alpha 1, gamma 2, T. alpha and T4 concentrations in OTA intoxicated rats. Meanwhile, a significant decrease in GSH, TAC, total protein and its fractions including (albumin, Total beta, Total gamma, Total globulin, alpha 2, beta 1, gamma 1), albumin/globulin (A/G) ratio in addition to serum testosterone, estradiol, TSH and T3 concentrations were observed in OTA exposed rats. The results suggested that NanoSe, probiotics, and vitamin C are strong antioxidants agents that might ameliorate OTA-induced renal and liver damage in rats by combating free radical and attenuate oxidative stress. Also, Nanoselenium, probiotics, and vitamin C, were found to be effective in protecting rats against OTA-induced hepato-renal injury by anti-inflammatory mechanism with the best result of Nano selenium or probiotic alone and their combination.","PeriodicalId":166243,"journal":{"name":"Damanhour Journal of Veterinary Sciences","volume":"13 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139265737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-15DOI: 10.21608/djvs.2022.165692.1096
Ali El-Far, Shaimaa M Saafan, Shymaa A. Mohamed, Fayza A El Tedawy, Abdelwahab Alsenosy
: Aging is one of the major risk factors associated chronic diseases. Oxidative stress is one of the aging associated mechanisms induced by pro-oxidants. Oxidative stress is an imbalance between free radicals and antioxidants in body cells. In the current study, we investigated the molecular docking scores of rutin with aging associated biomarkers, including human β -galactosidase, caspase-8, caspase-9, caspase-3, phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta (PK3CD), phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma (PK3CG), and RAC-alpha serine/threonine-protein kinase (AKT1). All proteins were retrieved from RCSB PDB database (https://www.rcsb.org/), while rutin three-dimensional structure was obtained from PubChem database (https://pubchem.ncbi.nlm.nih.gov/). The docking process was achieved by InstaDock software (https://instadock.webs.com/) and visualized by BIOVIA Discovery Studio Visualizer software. Rutin exhibited binding affinity (p Ki ) against AKT1, PK3CD, PK3CG, β -galactosidase, caspase-8, caspase-9, and caspase-3 of 7.85, 7.04, 6.89, 6.82, 5.94, 5.57, and 5.50, respectively. The obtained data revealed that rutin exhibited marked binding affinity to these biomarkers revealing its anti-aging potential.
{"title":"Rutin Attenuates Oxidative Stress Associated with Aging: Molecular Docking Study","authors":"Ali El-Far, Shaimaa M Saafan, Shymaa A. Mohamed, Fayza A El Tedawy, Abdelwahab Alsenosy","doi":"10.21608/djvs.2022.165692.1096","DOIUrl":"https://doi.org/10.21608/djvs.2022.165692.1096","url":null,"abstract":": Aging is one of the major risk factors associated chronic diseases. Oxidative stress is one of the aging associated mechanisms induced by pro-oxidants. Oxidative stress is an imbalance between free radicals and antioxidants in body cells. In the current study, we investigated the molecular docking scores of rutin with aging associated biomarkers, including human β -galactosidase, caspase-8, caspase-9, caspase-3, phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta (PK3CD), phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma (PK3CG), and RAC-alpha serine/threonine-protein kinase (AKT1). All proteins were retrieved from RCSB PDB database (https://www.rcsb.org/), while rutin three-dimensional structure was obtained from PubChem database (https://pubchem.ncbi.nlm.nih.gov/). The docking process was achieved by InstaDock software (https://instadock.webs.com/) and visualized by BIOVIA Discovery Studio Visualizer software. Rutin exhibited binding affinity (p Ki ) against AKT1, PK3CD, PK3CG, β -galactosidase, caspase-8, caspase-9, and caspase-3 of 7.85, 7.04, 6.89, 6.82, 5.94, 5.57, and 5.50, respectively. The obtained data revealed that rutin exhibited marked binding affinity to these biomarkers revealing its anti-aging potential.","PeriodicalId":166243,"journal":{"name":"Damanhour Journal of Veterinary Sciences","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139272551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-04DOI: 10.21608/djvs.2023.200798.1112
Naglaa F. Elbaz, Mohammad E. Abd Al Fatah, A. Gaafar, M. Soliman
{"title":"Investigations on virulence genes of some bacterial infections in Nile tilapia in Kafr El-Sheikh farms","authors":"Naglaa F. Elbaz, Mohammad E. Abd Al Fatah, A. Gaafar, M. Soliman","doi":"10.21608/djvs.2023.200798.1112","DOIUrl":"https://doi.org/10.21608/djvs.2023.200798.1112","url":null,"abstract":"","PeriodicalId":166243,"journal":{"name":"Damanhour Journal of Veterinary Sciences","volume":"93 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134409444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-07DOI: 10.21608/djvs.2022.157375.1090
S. Awad, Mohammed Abd El Hafez, Miral Abd Elwahab, Rehab Mady, G. Batiha, Hazem Shaheen, Doaa Ghareeb
Holothuria atra is a marine invertebrate found in the tropical indo-pacific region. It contains potent bioactive compounds, which include lectin, steroidal saponins
{"title":"Anti-inflammatory properties of Holothuria atra extract on lipopolysaccharide induced inflammation of White blood cells","authors":"S. Awad, Mohammed Abd El Hafez, Miral Abd Elwahab, Rehab Mady, G. Batiha, Hazem Shaheen, Doaa Ghareeb","doi":"10.21608/djvs.2022.157375.1090","DOIUrl":"https://doi.org/10.21608/djvs.2022.157375.1090","url":null,"abstract":"Holothuria atra is a marine invertebrate found in the tropical indo-pacific region. It contains potent bioactive compounds, which include lectin, steroidal saponins","PeriodicalId":166243,"journal":{"name":"Damanhour Journal of Veterinary Sciences","volume":"83 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122629882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：