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Malting and Wort Production Potential of the Novel Grain Kernza (Thinopyrum intermedium) 新型谷物Kernza (Thinopyrum intermedium)的酿造及麦汁生产潜力
IF 2 4区 农林科学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2022-03-16 DOI: 10.1080/03610470.2022.2026662
Andrew Marcus, Glen P. Fox
Abstract As the environmental impacts of beer are of increasing concern to maltsters, brewers, and consumers, perennial cereal crops can offer a more sustainable solution. One new cereal species of interest to the brewing industry is intermediate wheatgrass (Thinopyrum intermedium subsp. intermedium), developed by The Land Institute, in Salina, Kansas, U.S.A., under the branded name Kernza®. It has been touted as a more sustainable alternative to barley and wheat in its requirements for less water and nutrient additions and reduced tilling of agricultural fields. To date, no published research has been performed to assess the potential for this grain for the malt and beer industries as a barley replacement. Here, the “M5” variety Kernza grain was micromalted and the finished malt was compared with both the raw grain and to a reference raw and malted barley (Hordeum vulgare L. var. Copeland). All samples were analyzed for starch gelatinization temperatures via differential scanning calorimetry, amino acid composition, alpha-amylase, diastatic power (DP), total and soluble nitrogen and protein, Kolbach Index (KI), extract, wort free alpha-amino nitrogen (FAN), wort β-glucan, wort color, pH, and clarity. The Kernza malt produced sufficient extract and FAN for typical fermentations with low β-glucan content. However, extract and FAN in the Kernza malts were lower than in the barley reference, and the Kernza worts exhibited higher levels of undesirable soluble protein and haze. Interestingly, the DP for both the raw and malted Kernza were similarly high, indicating that both of the raw grains exhibit high enzymatic activity, which malting did not increase substantially. Results support that Kernza could be an acceptable candidate for malting and subsequent wort production; however, specific techniques may be required to utilize this malt most effectively. Supplemental data for this article is available online at https://doi.org/10.1080/03610470.2022.2026662 .
随着啤酒对环境的影响越来越受到酿酒师、酿酒商和消费者的关注,多年生谷物作物可以提供一个更可持续的解决方案。酿造工业感兴趣的一种新的谷物是中间小麦草(Thinopyrum intermedium subsp)。intermedium),由美国堪萨斯州萨利纳的土地研究所开发,品牌为Kernza®。它被吹捧为大麦和小麦更可持续的替代品,因为它需要更少的水和营养补充,减少了农田的耕作。到目前为止,还没有发表的研究来评估这种谷物作为大麦替代品在麦芽和啤酒工业中的潜力。在这里,“M5”品种Kernza谷物被微麦芽化,成品麦芽与原料谷物和参考原料和麦芽(Hordeum vulgare L. var. Copeland)进行比较。通过差示扫描量热法分析所有样品的淀粉糊化温度、氨基酸组成、α -淀粉酶、散散力(DP)、总氮和可溶性氮和蛋白质、科尔巴赫指数(KI)、提取物、麦汁游离α -氨基氮(FAN)、麦汁β-葡聚糖、麦汁颜色、pH和透明度。对于典型的β-葡聚糖含量低的发酵,克恩扎麦芽产生了足够的提取物和FAN。然而,克恩扎麦芽的提取物和FAN含量低于参考大麦,克恩扎麦芽表现出更高水平的不良可溶性蛋白和雾度。有趣的是,生的和麦芽的Kernza的DP都同样高,这表明两种生谷物都表现出很高的酶活性,而麦芽并没有显著增加。结果表明,Kernza可以作为麦芽和后续麦汁生产的可接受的候选品种;然而,为了最有效地利用这种麦芽,可能需要特定的技术。本文的补充数据可在https://doi.org/10.1080/03610470.2022.2026662上在线获得。
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引用次数: 3
Malting Performance of Landrace Barley and Wheat Grown in Nigeria Using Long and Short Steeping Regimes 尼日利亚长白大麦和小麦在长、短浸泡条件下的酿造性能
IF 2 4区 农林科学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2022-03-14 DOI: 10.1080/03610470.2022.2035575
R. Agu, O. Amadi, T. Nwagu, A. Moneke, B. Okolo
Abstract A longer steeping regime is advocated when malting elite barleys. A shorter steeping regime used in this study produced good hot water extract (HWE), fermentability, fermentable extract (FE), free amino nitrogen (FAN), peptide nitrogen (PN), and predicted spirit yield (PSY) from malted and mashed barley and wheat. High germination energy (GE)–germination test and good germination performance during the malting process resulted in the production of good quality malt. A high nitrogen content (less starch), lower screening of grains >2.5 mm (thin grains) of barley and wheat, and especially, the high husk (14.8%) of the barley further confirmed that mashing thin grains can cause lower PSY from the malted barley or wheat. The steeping regime influenced glucose to maltose ratios, resulting in higher glucose to maltose ratios. The glucose to maltose ratio is important for effective yeast fermentation. Complimentary results from FAN and PN supported yeast fermentation. Malted barley gave higher PSY results at both steeping regimes. Malted Nigerian-grown wheat gave higher PSY results than imported wheat, when short or longer steeping was used in malt production. This could be linked to differences in the starch structures in the wheat samples studied. The shorter steeping produced good quality malt. This could save costs in terms of energy use, water use, and carbon dioxide release from respiring embryos during steeping and germination. For optimum advantage of the shorter stepping regime to be achieved, good quality grain must be selected to reap the full benefit in using a shorter steeping regime to produce good malt.
在酿造优质大麦时,建议采用较长的浸泡时间。本研究采用较短的浸泡时间,从麦芽和捣碎的大麦和小麦中获得了良好的热水浸出物(HWE)、发酵性、可发酵浸出物(FE)、游离氨基氮(FAN)、肽氮(PN)和预测酒精产量(PSY)。高发芽能(GE) -发芽试验和良好的发芽性能是生产优质麦芽的关键。大麦和小麦的高含氮量(淀粉含量少)、低筛分率(粗粒)(粗粒)(粗粒)(粗粒)(粗粒)(粗粒)(粗粒)(粗粒)(粗粒)(粗粒)(粗粒)(粗粒)(粗粒)(14.8%)进一步证实,粗粒粉碎可使麦芽或小麦的粗粒PSY降低。浸泡制度影响葡萄糖与麦芽糖的比例,导致更高的葡萄糖与麦芽糖的比例。葡萄糖与麦芽糖的比例对有效的酵母发酵很重要。来自FAN和PN的补充结果支持酵母发酵。在两种浸泡制度下,麦芽的PSY结果更高。当在麦芽生产中使用短时间或长时间浸泡时,尼日利亚种植的麦芽的PSY结果高于进口小麦。这可能与所研究的小麦样品中淀粉结构的差异有关。浸泡时间较短,麦芽质量较好。这可以节省能源的使用,水的使用,以及在浸泡和萌发过程中呼吸胚胎释放的二氧化碳的成本。为了实现较短的步进制度的最佳优势,必须选择优质的谷物,以便在使用较短的浸泡制度生产优质麦芽时获得充分的好处。
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引用次数: 1
Breeding of New Saccharomyces cerevisiae Hybrids with Reduced Higher Alcohol Production for Light-Aroma-Type-Xiaoqu Baijiu Production 低酒精度酿酒酵母新杂交种的选育用于轻香型小曲白酒生产
IF 2 4区 农林科学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2022-03-04 DOI: 10.1080/03610470.2022.2033608
Shenxi Chen, Gemma Perez-Samper, Beatriz Herrera-Malaver, Liping Zhu, Yuancai Liu, Jan Steensels, Qiang Yang, K. Verstrepen
Abstract Baijiu, also referred to as "Chinese liquor", is a traditional spirit that originated in China over 2000 years ago and is the world’s bestselling liquor today. It is produced using a solid-state fermentation process, often using sorghum as raw material, in which a complex microbial cocktail (called "Jiuqu") is introduced to initiate the fermentation process. During fermentation, a plethora of flavor-active metabolites is produced by microbes. Depending on the flavors formed, Baijiu can be subdivided into sauce-aroma-type, strong-aroma-type, and light-aroma-type. A key feature of light-aroma-type Xiaoqu Baijiu (LATXB) is its relatively low concentration of higher alcohols (HA). However, reaching sufficiently low HA concentrations without affecting the overall taste and flavor of LATXB is often difficult. In this project, breeding was used to develop a new Saccharomyces cerevisiae hybrid strain for LATXB production, which shows reduced HA production. This strain, dubbed “hybrid 5”, reduced total HA by 10.3%, without compromising the production of other metabolites or fermentation efficiency when evaluated at an industrial scale (2 tons). Together, these results show the potential of breeding to produce superior, non-GM yeasts that are directly applicable for commercial Baijiu production.
白酒,又称“中国白酒”,是一种2000多年前起源于中国的传统烈酒,是当今世界上最畅销的白酒。它采用固态发酵工艺生产,通常以高粱为原料,其中引入复杂的微生物鸡尾酒(称为“酒曲”)来启动发酵过程。在发酵过程中,微生物产生了大量的风味活性代谢物。根据形成的风味,白酒可分为酱香型、浓香型和淡香型。轻香型小曲白酒的一个重要特征是其较高醇(HA)的浓度相对较低。然而,达到足够低的HA浓度而不影响LATXB的整体味道和风味通常是困难的。本项目采用选育的方法,开发了一种生产LATXB的新型酿酒酵母杂交种,其HA产量降低。该菌株被称为“杂交5”,在工业规模(2吨)评估时,将总HA减少了10.3%,而不影响其他代谢物的生产或发酵效率。总之,这些结果表明,培育出可直接用于商业白酒生产的优质非转基因酵母的潜力。
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引用次数: 3
Isolation, Selection and Characterization of Wild Yeasts with Potential for Brewing 具有酿酒潜力的野生酵母的分离、选择与特性研究
IF 2 4区 农林科学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2022-03-02 DOI: 10.1080/03610470.2022.2031777
Fernanda Otesbelgue Pinto, T. Lopes, Audren Monteiro Vieira, Renata Ott Oliveira, F. F. Gomes, M. F. Fabricio, M. A. Z. Ayub, S. Mendes, D. M. Pagani, P. Valente
Abstract Ninety-two wild yeasts were isolated from different fruits, for possible use in brewing, using the technological traits of carbohydrate fermentation and hydrogen sulfide production. Twelve yeasts were selected for identification by sequencing (D1/D2 domain or ITS1-5.8S-ITS2 region) and their volatile compounds production analyzed using gas chromatography. From these analyses, three yeasts were chosen for further characterization and beer production. Strains were characterized by their tolerance to osmotic and ethanol stress, their flocculation profile, growth at different temperatures, apparent attenuation (percentage of sugars converted into alcohol), estimated ABV (alcohol by volume) and reducing sugar profile. Beers were analyzed for alcohol content and sensorial analysis. The isolates CL011 and PB111 (Saccharomyces cerevisiae) and CB341 (Wickerhamomyces anomalus) showed promising properties for brewing, which included high production of interesting volatile compounds (esters, terpenes, lactones, and C13-norisoprenoids) by CL011, a neutral profile by PB111, and the production of non-classic beer volatile compounds by CB341 in a mixed culture. Supplemental data for this article is available online at https://doi.org/10.1080/03610470.2022.2031777 .
摘要利用碳水化合物发酵和硫化氢生产的工艺特点,从不同的水果中分离出92株野生酵母,可用于酿造。选择12种酵母进行测序鉴定(D1/D2结构域或ITS1-5.8S-ITS2区域),并使用气相色谱分析其挥发性化合物的产生。从这些分析中,选择了三种酵母进行进一步的表征和啤酒生产。菌株的特征是它们对渗透和乙醇胁迫的耐受性、絮凝特性、在不同温度下的生长、表观衰减(糖转化为酒精的百分比)、估计的ABV(按体积计算的酒精)和还原糖特性。对蜜蜂进行了酒精含量和感官分析。分离株CL011和PB111(酿酒酵母)和CB341(异头威卡霉)显示出良好的酿酒性能,包括CL011产生大量有趣的挥发性化合物(酯类、萜烯类、内酯类和C13-去异戊二烯类),PB111具有中性特性,CB341在混合培养基中产生非经典啤酒挥发性化合物。本文的补充数据可在线获取,网址为https://doi.org/10.1080/03610470.2022.2031777。
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引用次数: 2
Exploring Fungal Species Diversity in the Premature Yeast Flocculation (PYF) of Barley Malt Using Deep Sequencing 利用深度测序技术研究大麦麦芽早酵母絮凝(PYF)过程中真菌物种多样性
IF 2 4区 农林科学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2022-03-02 DOI: 10.1080/03610470.2021.2025329
Qingqing Qin, Jia Liu, Shumin Hu, Junhong Yu, Lei Zhang, Shuxia Huang, Mei Yang, Lushan Wang
Abstract Premature yeast flocculation (PYF) is the early flocculation of yeast during fermentation, which causes logistical and financial hardship to the malting and brewing industries. The occurring of PYF is suspected to be caused by the fungal infection of the barley or at least in part. The aim of this study was to perform a non-culture approach to uncover the fungal diversity in PYF malt. The genomic DNA was extracted and analyzed by amplicon sequencing. A total pairs read number of 209,523 sequences was obtained and used for taxonomic classification. The dominant fungal communities on the PYF malt were obtained based on the microbial diversity analysis by species annotation. The internal transcribed spacers sequencing analysis showed a decreased number of fungal species in PYF malt. Results showed that compared with control malt, PYF malt displayed a higher abundance of Ascomycota at the phyla level, and the abundance of Candida and Gibberella was exceptionally high in two PYF malts at the genus level. The absolute abundance of Candida and Gibberella baccata at the species level was also higher in PYF malts. The fungal species diversity within the malt indicated that the fungi community diversity was greater in the control malt than in the PYF malt. Taken together, exploring the diversity of the fungal community could be useful for malt quality control and for eliminating contamination risks.
摘要酵母早絮凝(PYF)是酵母在发酵过程中的早期絮凝现象,给麦芽和酿酒行业带来了物流和经济上的困难。PYF的发生被怀疑是由大麦的真菌感染引起的,或者至少部分是这样。本研究的目的是执行非培养方法,以揭示真菌多样性的PYF麦芽。提取基因组DNA并进行扩增子测序分析。共获得209,523对序列,并用于分类分类。通过物种注释的微生物多样性分析,获得了PYF麦芽的优势真菌群落。内部转录间隔序列分析显示,PYF麦芽中真菌种类数量减少。结果表明,与对照麦芽相比,PYF麦芽在门水平上显示出更高的子囊菌群丰度,在属水平上,两种PYF麦芽的念珠菌和赤霉素丰度异常高。在种水平上念珠菌和巴卡赤霉素的绝对丰度在PYF麦芽中也较高。麦芽内真菌种类多样性表明,对照麦芽真菌群落多样性大于PYF麦芽。总之,探索真菌群落的多样性可能有助于麦芽质量控制和消除污染风险。
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引用次数: 1
Evaluation of Commercial Strains of Torulaspora delbrueckii in Beer Production 啤酒生产中商品菌株的评价
IF 2 4区 农林科学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2022-02-15 DOI: 10.1080/03610470.2021.2025327
Fotini Drosou, Katerina Anastasakou, P. Tataridis, V. Dourtoglou, V. Oreopoulou
Abstract Nowadays, the micro-brewing industry aims at yeast strains beyond Saccharomyces cerevisiae, the so-called non-Saccharomyces, for their flavor contribution to the final product. In the present study, two commercial strains of Torulaspora delbrueckii (Prelude and 291) were evaluated for the production of a pale ale beer, in mono- and co-culture fermentations with S. cerevisiae, at 13 and 20 °C. From the results, it was concluded that both non-Saccharomyces yeasts were able to metabolize wort sugars at both temperatures, albeit at a slower rate at 13 °C. The fermented products were sealed in glass bottles (without filtration) and measurements of the ethanol content, color, bitterness, and volatile compounds were conducted after 30 days of storage. The ethanol content obtained from the non-Saccharomyces monocultures varied between 3.90 and 5.50% alcohol by volume (ABV), while that of the co-cultures was not significantly different from the ethanol content obtained by the conventional yeast (4.80–5.80% ABV). Higher bitterness and color values were detected in the non-Saccharomyces fermentations. The analysis of volatile compounds revealed that the two non-conventional yeasts were capable of producing aromatic components, such as higher alcohols and esters, yielding a fruity and floral aromatic profile, especially in the mixed culture fermentation of T. delbrueckii 291 at 13 °C.
摘要如今,微酿酒工业的目标是酿酒酵母以外的酵母菌株,即所谓的非酿酒酵母,因为它们对最终产品的风味贡献。在本研究中,在13岁和20岁时,在与酿酒酵母的单培养和共培养发酵中,评估了两种德氏Torulaspora delbrueckii商业菌株(Prelude和291)生产淡啤酒的能力 °C。从结果中可以得出结论,两种非酿酒酵母都能够在两种温度下代谢麦芽糖,尽管在13℃时代谢速度较慢 °C。将发酵产物密封在玻璃瓶中(无需过滤),并在30分钟后测量乙醇含量、颜色、苦味和挥发性化合物 储存天数。从非酿酒酵母单培养物中获得的乙醇含量在3.90至5.50%酒精体积(ABV)之间变化,而共培养物的乙醇含量与传统酵母获得的乙醇浓度(4.80至5.80%ABV)没有显著差异。在非酿酒酵母发酵中检测到较高的苦味和色度值。挥发性化合物的分析表明,这两种非传统酵母能够产生芳香成分,如高级醇和酯类,产生果味和花香,尤其是在T.delbrueckii 291在13 °C。
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引用次数: 2
Use of Yeasts in Traditional Alcoholic Beverages in Tanzania and Potential Opportunities 酵母在坦桑尼亚传统酒精饮料中的使用及其潜在机遇
IF 2 4区 农林科学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2022-02-15 DOI: 10.1080/03610470.2021.2013677
Christian Brayson Tarimo, L. Kaale
Abstract Alcoholic beverages are produced in many parts of the world for different purposes, including refreshment and people’s cultural and religious practices. The advancement in their production technology has evolved over time due to a number of factors, including the availability of modern equipment, innovation, operating costs, and customer competition. In Africa, particularly Tanzania, different types of traditional beverages are produced. These beverages have been recognized for their contribution to households’ economy, especially in rural and semi-urban areas. However, limited strategic measures have been implemented to realize their potential in local and international markets. Among the major concerns under this category is the source and management of fermentation cultures in use, which affect the quality, safety, consistency, and shelf life of the final products. Fermentation is the basis of almost all alcoholic beverages. It involves the use of fermenting cultures containing a mixture of microorganisms, often yeast, lactic acid bacteria, and molds. The sources of these traditional fermenting cultures are mainly a fermented porridge of cereals and banana juice, a portion of a previous brew, or dried yeast harvested from a previous brew. This review discusses the potential of the use of culture yeast in adding value to traditional alcoholic beverages, especially in terms of quality and shelf life. Yeast selection, yeast management, waste yeast management, and the role of quality assurance in yeast management are covered.
世界上许多地方都生产酒精饮料,用于不同的目的,包括提神和人们的文化和宗教习俗。由于许多因素,包括现代设备的可用性,创新,运营成本和客户竞争,他们的生产技术的进步随着时间的推移而发展。在非洲,特别是坦桑尼亚,生产不同类型的传统饮料。这些饮料因其对家庭经济的贡献而得到认可,特别是在农村和半城市地区。但是,为发挥它们在当地和国际市场上的潜力而采取的战略措施有限。这一类别下的主要问题是使用中的发酵培养物的来源和管理,这影响最终产品的质量、安全性、一致性和保质期。发酵是几乎所有酒精饮料的基础。它包括使用含有微生物混合物的发酵培养物,通常是酵母、乳酸菌和霉菌。这些传统发酵文化的来源主要是谷物和香蕉汁的发酵粥,以前酿造的一部分,或者从以前酿造的酿造中收获的干酵母。本文讨论了利用培养酵母增加传统酒精饮料附加值的潜力,特别是在质量和保质期方面。酵母的选择,酵母管理,废酵母管理,以及酵母管理质量保证的作用。
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引用次数: 2
Monitoring of Glycosidically Bound Polyphenols in Hops and Hop Products Using LC-MS/MS Technique 用LC-MS/MS技术监测啤酒花及其制品中的糖苷结合多酚
IF 2 4区 农林科学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2022-02-10 DOI: 10.1080/03610470.2021.2024112
M. Biendl, S. Ritter, C. Schmidt
Abstract Recent findings have shown that besides bitter acids, several additional hop ingredients contribute to beer bitterness. These belong to the class of polyphenols and are also part of the hard resin fraction of hops. They include the two well-known flavonols, quercetin and kaempferol, widespread in the plant kingdom. On the other hand, the so-called multifidols are more typical to hops as intermediates in the biosynthesis of alpha- and beta-acids. Both of the above mentioned flavonols and the multifidols occur mainly not in their free forms but rather bonded to sugars such as glucose. In our study, the contents of such glycosidically bound polyphenols were determined in a worldwide spectrum of different hop varieties using liquid chromatography in combination with tandem mass spectrometry. In total, more than 500 samples from crops 2020 and 2019, representing 34 different varieties, were analyzed. The detected concentrations (mg/100 g) were dependent on variety, with ranges between of 6–155 for co-multifidol glucoside, 3–60 for ad-multifidol glucoside, 13–87 for quercetin-3-O-glucoside, 5–46 for quercetin-3-O-rutinoside, and 4–35 for kaempferol-3-O-glucoside. Moreover, their fate during large scale hop extraction was investigated. It was confirmed that polyphenol glycosides were not extracted with supercritical carbon dioxide but were extracted with ethanol.
摘要最近的研究结果表明,除了苦味酸之外,啤酒花中的几种额外成分也会导致啤酒苦味。这些属于多酚类,也是啤酒花硬树脂部分的一部分。它们包括两种著名的黄酮醇,槲皮素和山奈酚,广泛存在于植物界。另一方面,所谓的多偶像更典型的是啤酒花作为α酸和β酸生物合成的中间体。上述黄酮醇和多聚体主要不以游离形式存在,而是与糖如葡萄糖结合。在我们的研究中,使用液相色谱法和串联质谱法在世界范围内不同啤酒花品种的光谱中测定了这种糖苷结合多酚的含量。总共分析了来自2020年和2019年作物的500多个样本,代表34个不同的品种。检测浓度(mg/100 g) 取决于品种,co-multipiol葡糖苷的范围为6-155,ad-multipiol葡萄糖苷为3-60,槲皮素-3-O-葡糖苷为13-87,槲皮素-3-O-rutinoside为5-46,山奈酚-3-O-葡萄糖苷为4-35。此外,还研究了它们在大规模啤酒花提取过程中的命运。证实多酚糖苷不是用超临界二氧化碳提取的,而是用乙醇提取的。
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引用次数: 2
Processing Condition Effects on Sensory Profiles of Kombucha through Sensory Descriptive Analysis 感官描述分析加工条件对康普茶感官性状的影响
IF 2 4区 农林科学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2022-02-09 DOI: 10.1080/03610470.2021.2022879
Katherine Phetxumphou, Rebecca Vick, Lori Blanc, Jacob Lahne
Abstract Kombucha is a fermented and often sweetened tea beverage that has become increasingly popular in the U.S. in the last decade. While it is often infused with fruits and vegetables to enhance aroma and flavor, unflavored kombucha itself has distinctive sensory attributes influenced by multiple factors, including initial tea type, initial sugar concentration, and fermentation temperature. How these different factors affect the final aroma and flavor of kombucha has been underexplored in the scientific literature. In this study, processing treatments, including tea type (black and green), sugar concentration (63 g/L and 94 g/L), and fermentation temperature (21 °C and 25.5 °C) were combined in a factorial design to produce experimental kombucha (K = 8). Panelists (N = 7) completed a full sensory Descriptive Analysis (DA) evaluation of the experimental kombucha, in which they defined 63 sensory attributes. MANOVA identified 43 sensory attributes that differed among the samples due to tea type, initial sugar concentration, and fermentation temperature, although surprisingly tea, sugar, and temperature did not interact significantly. Overall, teas fermented at a lower temperature or with higher sugar were sweeter, thicker, and associated with fruit-related aromatics; teas fermented at a higher temperature and with less sugar were associated with fermented, vegetal, and yeasty aromas, fizzy mouthfeels, and sour and bitter tastes. Surprisingly, tea type played only a weak role in final kombucha flavor.
康普茶是一种发酵的加糖茶饮料,在过去十年中在美国越来越受欢迎。虽然经常加入水果和蔬菜来增强香气和风味,但无味的康普茶本身具有独特的感官属性,受多种因素的影响,包括初始茶叶类型,初始糖浓度和发酵温度。这些不同的因素是如何影响康普茶的最终香气和味道的,在科学文献中还没有得到充分的研究。在本研究中,采用因子设计,将加工处理,包括茶叶类型(黑色和绿色),糖浓度(63 g/L和94 g/L),发酵温度(21℃和25.5℃)相结合,生产实验康普茶(K = 8)。小组成员(N = 7)完成了对实验康普茶的完整感官描述性分析(DA)评估,其中他们定义了63种感官属性。MANOVA发现了43种不同的感官属性,这是由于茶叶类型、初始糖浓度和发酵温度的不同,尽管令人惊讶的是,茶、糖和温度并没有显著的相互作用。总体而言,在较低温度或较高糖发酵的茶更甜,更浓稠,并与水果相关的芳香有关;在较高温度和较少糖发酵的茶与发酵,植物和酵母的香气,起泡的口感以及酸味和苦味有关。令人惊讶的是,茶的种类在最终的康普茶味道中只起了微弱的作用。
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引用次数: 4
Iron Enhances the Growth of the Genus Pectinatus in Beer 铁促进啤酒中果胶属植物的生长
IF 2 4区 农林科学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2022-01-26 DOI: 10.1080/03610470.2021.2019554
Nobuchika Takesue, Koji Suzuki, Masanori Mizutani, Yuichi Nakamura
Abstract To determine factors affecting the growth of the genus Pectinatus in beer, P. frisingensis ABBC437 was inoculated into 15 different beer samples. Although the pH, alcohol content, and bitterness unit of the 15 beer samples were adjusted to similar values within tolerance, growth kinetics as expressed by OD600 (optical density measured at a wavelength of 600 nm) were considerably different among the beer samples, indicating the presence of “as yet” unidentified factor(s) affecting the growth of P. frisingensis in beer. Chemical compositions of the beer samples were determined, and multiple regression analysis was conducted to predict the OD600 value. As a result, iron was suggested as one of the factors that affects the growth of P. frisingensis in beer because the additions of iron to the beer samples showed enhanced growth of P. frisingensis in a dose-dependent manner. Stimulatory effects of growth by the addition of iron were also observed for P. cerevisiiphilus and P. haikarae. Additionally, effects of diatomaceous earth treatment on the iron concentrations of the beer samples were evaluated. Iron concentrations varied, depending on the brand of diatomaceous earth, and the growth of P. frisingensis in beer showed a high correlation coefficient (0.924, p < 0.01) with the iron concentration in the beer samples. From these results, iron can be considered as an important factor for the growth of the genus Pectinatus in beer and selecting a low iron concentration diatomaceous earth is an effective way to improve the stability of beer against the risk of spoilage by the genus Pectinatus.
摘要为了确定影响果胶酶属在啤酒中生长的因素,将弗氏葡萄球菌ABBC437接种到15个不同的啤酒样品中。尽管15个啤酒样品的pH、酒精含量和苦味单位被调节到公差范围内的相似值,但OD600表示的生长动力学(在600波长下测量的光密度 nm)在啤酒样品中有显著差异,表明存在影响弗氏假单胞菌在啤酒中生长的“迄今为止”未知因素。测定啤酒样品的化学成分,并进行多元回归分析以预测OD600值。因此,铁被认为是影响啤酒中弗氏假单胞菌生长的因素之一,因为向啤酒样品中添加铁以剂量依赖的方式增强了弗氏假单孢菌的生长。此外,还观察到添加铁对蜡状芽孢杆菌(P.cerevisiphilus)和海葵(P.haikarae)生长的刺激作用。此外,还评估了硅藻土处理对啤酒样品中铁浓度的影响。铁浓度随硅藻土品牌的不同而变化,啤酒中弗里辛根霉的生长表现出较高的相关系数(0.924,P < 0.01)与啤酒样品中的铁浓度的关系。从这些结果来看,铁可以被认为是果胶酶属在啤酒中生长的重要因素,选择低铁浓度的硅藻土是提高啤酒稳定性以抵御果胶酶属腐败风险的有效途径。
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Journal of the American Society of Brewing Chemists
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