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Near-complete genome sequence of a reassortant H1N2 swine influenza virus isolated from a pig in the Republic of Korea in 2016 2016年从韩国一头猪身上分离出的重组型H1N2猪流感病毒的近乎完整的基因组序列
Pub Date : 2021-06-30 DOI: 10.13041/jpvm.2021.45.2.84
I. Ouh, Juyeon Lee, Suyeong Yun, Hyeon Choi, S. Moon, Min Ji Kim, B. Hyun, Yu-Ri Park, Jienny Lee, Yoon-Hee Lee, Choi-Kyu Park
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引用次数: 0
Estimating the basic reproduction number for the 2019/20 African swine fever epidemics in wild boars in the Republic of Korea 估计2019/20年韩国野猪中非洲猪瘟流行的基本繁殖数
Pub Date : 2021-06-30 DOI: 10.13041/jpvm.2021.45.2.57
Eu-Tteum Kim, S. Pak
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引用次数: 1
Comparison of characteristics of mesenchymal stem cells from bovine 牛间充质干细胞特性比较
Pub Date : 2021-06-30 DOI: 10.13041/jpvm.2021.45.2.63
N. Gu, So Yeon Jeong, Se-A Lee, Seong-In Lim, Jienny Lee, Yoon-Hee Lee, B. Hyun
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引用次数: 0
Immunogenicity of replication-defective recombinant vesicular stomatitis virus expressing Middle East respiratory syndrome coronavirus spike protein in mice 表达中东呼吸综合征冠状病毒刺突蛋白的复制缺陷重组水泡性口炎病毒在小鼠体内的免疫原性
Pub Date : 2021-06-30 DOI: 10.13041/jpvm.2021.45.2.70
Jung-Eun Park
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引用次数: 0
Recurrent nodular fasciitis in young dogs: reports of two cases 幼犬复发性结节性筋膜炎:两例报告
Pub Date : 2021-06-30 DOI: 10.13041/jpvm.2021.45.2.88
J. Kong, M. Seo, Yongsoo Choi, Chang-Hee Lee, I. Hong
Two dogs presented with nodular masses on the head and scapula. The masses recurred after excision and were submitted for histopathological examination. Macroscopically, hemorrhage and necrosis were apparent on the cut surface. Microscopically, the lesions were poorly demarcated and incorporated subcutaneous fat and adjacent skeletal muscle fibers. Fibrocytes and fibroblasts were admixed and haphazardly arranged. Both were diagnosed with nodular fasciitis (NF). NF has been rarely reported and called as a ‘pseudosarcomatous’ lesion because of its infiltrative growth and cellular pleomorphism. The present report describes unusual cases of recurrent NF in two young dogs based on histopathologic and clinical features.
两只狗的头部和肩胛骨出现结节性肿块。肿物切除后复发,行组织病理学检查。肉眼可见切口表面明显出血和坏死。显微镜下,病变界限不清,合并皮下脂肪和邻近的骨骼肌纤维。成纤维细胞和成纤维细胞混杂,排列杂乱无章。两例均诊断为结节性筋膜炎(NF)。由于其浸润性生长和细胞多形性,NF很少被报道并被称为“假肉瘤”病变。本报告描述了不寻常的情况下,复发性NF在两个年轻的狗基于组织病理学和临床特征。
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引用次数: 0
Characterization of monoclonal antibodies against antigenic epitopes of Actinobacillus pleuropneumoniae ApxIVA 胸膜肺炎放线杆菌ApxIVA抗原表位单克隆抗体的鉴定
Pub Date : 2021-06-30 DOI: 10.13041/jpvm.2021.45.2.97
M. W. Oh, Suji Kim, H. Yoo
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引用次数: 0
Fungicidal efficacy of a disinfectant solution composed of chlorine dioxide and quaternary ammonium compound against Aspergillus flavus and Aspergillus fumigatus caused stonebrood in honey bees 二氧化氯和季铵化合物组成的消毒液对蜜蜂黄曲霉和烟曲霉产石茧的杀菌效果
Pub Date : 2021-06-30 DOI: 10.13041/jpvm.2021.45.2.93
Chun-Nam Cha, Eun‐Kee Park, Suk Kim, Hu-Jang Lee
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引用次数: 0
A preliminary investigation into the decomposition rate of wild boar carcasses in forest habitats 森林生境中野猪尸体分解率的初步调查
Pub Date : 2021-03-31 DOI: 10.13041/JPVM.2021.45.1.44
Hee-Kyeung Cho, E. Kim, Bong S Jung, S. Pak
The estimation of the postmortem interval (PMI, the time that has elapsed since the death) is a critical issue for the biosecurity enforcement officers who implement to the timely establishment of biosecurity zone for preventing susceptible animals from disease transmission given the rapid occurrence of an infectious disease. Increasing attention has been paid to PMI of wild boar (Sus scrofa) carcasses associated with African swine fever epidemics in Korea since October 2019 to explain the geographical transmission of the disease, as well as to provide potential target animals for prevention measures in terms of farm biosecurity. This study is, to the best of our knowledge, the first to describe the decomposition process of wild boars in different microhabitats in the country. In the present comparative study, we obtained field data from the decomposition process of the wild boar and domestic pig carcasses continuously exposed aboveground in natural environment settings allowing animal scavenging. This study compared the pattern of decomposition in 16 wild boar carrion and 10 domestic pig carcasses placed between August and November 2019. Quite differences in decomposition rate measured by total body score and tissue's gross morphology over time were observed between wild boars and domestic pigs. Overall, the wild boar decomposed much more slowly than the domestic pig throughout the end of the experimental period. In addition, color changes to the skin were easily seen in domestic pigs, whereas there is much variation in the wild boar, especially carcasses placed in late autumn. Moreover, some wild boar carcasses did not show any sign of bloating. These results indicate that decomposition rates derived from forensic taphonomic studies on domestic pigs may be not directly applicable to the wild boar, hence there is a need to develop regional decomposition models to be employed in different geographical situations to increase the accuracy of PMI of wild boar carcasses.
在传染病迅速发生的情况下,为了及时建立防止易感动物传播疾病的生物安全区,对生物安全执法人员来说,估计死亡时间(PMI,即死亡后的时间)是一个关键问题。自2019年10月以来,人们越来越关注与韩国非洲猪瘟疫情相关的野猪(Sus scrofa)尸体的PMI,以解释该疾病的地理传播,并为农场生物安全方面的预防措施提供潜在目标动物。据我们所知,这项研究首次描述了该国不同微栖息地中野猪的分解过程。在本比较研究中,我们从野猪和家猪尸体在允许动物食腐的自然环境中持续暴露在地面上的分解过程中获得了现场数据。这项研究比较了2019年8月至11月期间放置的16具野猪腐肉和10具家猪尸体的分解模式。野猪和家猪的分解率随时间的变化在身体总分和组织大体形态上有很大的差异。总体而言,在整个试验期结束时,野猪的腐烂速度比家猪慢得多。此外,家猪的皮肤颜色变化很容易看到,而野猪的皮肤颜色变化很大,特别是在深秋放置的尸体。此外,一些野猪的尸体没有任何肿胀的迹象。这些结果表明,从家猪的法医埋藏学研究中得出的分解率可能并不直接适用于野猪,因此需要开发适用于不同地理情况的区域分解模型,以提高野猪尸体PMI的准确性。
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引用次数: 3
AmpC β-Lactamase producing Proteus mirabilis isolates from dogs and cats in South Korea 产生AmpC β-内酰胺酶的神奇变形杆菌从韩国的狗和猫中分离出来
Pub Date : 2021-03-31 DOI: 10.13041/JPVM.2021.45.1.18
Christine Kim, W. Jung, Se Ra Shin, Seong Mi Noh, Yu Jin Yang, Sook Shin, K. Park, Y. Park
Resistance to extended-spectrum cephalosporin in Enterobacteriaceae is increasingly prevalent in South Korea. This study aims to explore the distribution of AmpC genes in Proteus mirabilis isolated from stray and hospital-admitted companion animals in South Korea. AmpC β-lactamases hold clinical significance due to its potential to facilitate antimicrobial resistance to cefoxitin, cefazolin, and most penicillins. A total of 163 bacterial isolates belonging to the Enterobacteriaceae family were collected from dogs (n = 158) and cats (n=5). Of them, 134 isolates were from hospital-admitted animals, while 29 isolates from stray animals. Boronic acid tests and antimicrobial susceptibility tests were conducted for an initial screening to detect AmpC β-lactamase resistant P. mirabilis. Gene-specific PCRs were conducted to identify the type of AmpC genes, which include six groups (MOXM, CITM, DHAM, ACCM, EBCM, and FOXM), in the resistant isolates. The boronic acid disk tests revealed 45 (27.6%) positive isolates out of 163 isolates tested. Of these 45 isolates, six were determined to harbor the EBCM gene, 13 for CITM, one for FOXM, and one for DHAM by single detection PCR. No isolates carried for ACCM or MOXM. Thus, a total of 21 out of 163 isolates (12.9%) were demonstrated to possess AmpC genes. No isolates contain more than one group of AmpC gene family. A significantly higher percentage of P. mirabilis was found to possess AmpC genes compared to past studies. Therefore, the increasing trend in antimicrobial resistance in P. mirabilis indicates a dire need to monitor antimicrobial prescription in the veterinary field.
在韩国,肠杆菌科对广谱头孢菌素的耐药性日益普遍。本研究旨在探讨从韩国流浪动物和住院伴侣动物中分离的奇异变形杆菌AmpC基因的分布。AmpC β-内酰胺酶具有临床意义,因为它有可能促进对头孢西丁、头孢唑林和大多数青霉素的耐药性。从狗(n= 158)和猫(n=5)身上共分离到163株肠杆菌科细菌。其中,134株分离株来自住院动物,29株分离株来自流浪动物。采用硼酸试验和药敏试验初步筛选AmpC β-内酰胺酶耐药奇异假单胞菌。采用基因特异性pcr技术鉴定耐药菌株AmpC基因类型,包括MOXM、CITM、DHAM、ACCM、EBCM和FOXM 6组。硼酸圆盘试验163株中45株(27.6%)呈阳性。45株分离株中,6株为EBCM基因,13株为CITM基因,1株为FOXM基因,1株为DHAM基因。没有分离株携带ACCM或MOXM。因此,163株菌株中有21株(12.9%)被证实具有AmpC基因。没有分离株含有多于一组AmpC基因家族。与过去的研究相比,发现奇异假单胞菌具有AmpC基因的比例显着提高。因此,P. mirabilis耐药性的增加趋势表明兽医领域迫切需要对抗菌药物处方进行监测。
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引用次数: 0
Comparison of antibody response between intradermal and intramuscular administration of a foot-and-mounth disease vaccine in pigs 猪口蹄疫疫苗皮内注射和肌肉注射的抗体反应比较
Pub Date : 2021-03-31 DOI: 10.13041/JPVM.2021.45.1.7
Sung-Hyun Choi, Byung-Il Jung, Y. Kim, Eu-Tteum Kim, S. Pak
Since the 2010 foot and mouth (FMD) epidemic, the Korean government has applied a FMD vaccination to pigs. A FMD vaccine is injected to pigs by intramuscular (IM) route. One of the drawbacks in FMD vaccine IM injection is that it would result in an abnormal meat on the injection spot. An abnormal meat due to FMD vaccine IM injection would cause economic loss to pig farmers. An intradermal (ID) injection would be an alternative method for FMD vaccination. The goal of current study was to compare the antibody formation rate between FMD vaccine IM injection and ID injection. The antibody formation rate was measured by the FMD serotype O vaccination percent inhibition (PI) value. In total 350 pigs (175 for FMD vaccine IM injection and 175 for FMD vaccine ID injection) were included in the study. In results, the PI values of FMD vaccine IM injection group were significantly higher than it of FMD vaccine ID injection group. However, the proportions of pigs with PI value was higher than 50, which is a legislative requirement for marketing pigs, for both FMD vaccine IM and ID injection groups at week 20 or 23 were not significantly different. The current results indicated that a FMD vaccine ID injection could be an alternative method of IM injection.
2010年发生口蹄疫疫情后,韩国政府对猪进行了口蹄疫疫苗接种。通过肌肉注射(IM)途径给猪注射口蹄疫疫苗。口蹄疫疫苗注射的缺点之一是注射部位会出现异常肉块。由于注射口蹄疫疫苗而导致的肉质异常会给养猪户造成经济损失。皮内注射是口蹄疫疫苗接种的另一种方法。本研究的目的是比较口蹄疫疫苗注射IM和注射ID的抗体形成率。抗体形成率采用口蹄疫血清O型疫苗接种百分比抑制(PI)值测定。试验共纳入350头猪,其中注射口蹄疫疫苗IM组175头,注射口蹄疫疫苗ID组175头。结果:口蹄疫疫苗IM注射组PI值显著高于口蹄疫疫苗ID注射组。但在第20周和第23周,注射口蹄疫疫苗IM组和注射ID组的PI值均高于立法要求的50,差异不显著。目前的研究结果表明,口蹄疫疫苗注射ID可作为注射IM的替代方法。
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Journal of the Preventive Veterinary Medicine
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