Pub Date : 2018-12-01DOI: 10.5958/0974-0813.2018.00018.9
S. Kordafshari, S. Hosseini, Hagos Gebrekidan, F. Jalousian, P. Shayan, M. Bandehpour, B. Kazemi, M. Rajabibazl, Malcolm K. Jones, S. Jafariaskari
Echinococcus granulosus is considered as a significant parasitic infection with worldwide distribution. The present study was aimed to express the diagnostic antigen EpC1 in a recombinant system and set up an indirect ELISA for diagnosis of infected canines. Hydatid cysts were collected from liver and lungs of naturally infected sheep from slaughter houses and cDNA was synthesized from the protoscoleces. The EpC1 was expressed as recombinant protein in E. coli using pET28a as expression vector and purified by His-tag affinity chromatography. Expression of recombinant EpC1 was confirmed using SDS-PAGE followed by Western immunoblotting using T7-Tag monoclonal antibody and sera from naturally infected and experimentally challenged dogs. The diagnostic potential of the recombinant EpC1 was assessed against 22 dog sera using indirect ELISA. The sensitivity, specificity, positive and negative prediction values of the ELISA test were calculated as 100%, 93.3%, 88.8% and 100%, respectively. Recombinant EpC1 showed promising results for the diagnosis of echinococcosis in dogs.
{"title":"Molecular cloning and expression of a recombinant epc1 protein for serodiagnosis of echinococcus granulosus in dogs","authors":"S. Kordafshari, S. Hosseini, Hagos Gebrekidan, F. Jalousian, P. Shayan, M. Bandehpour, B. Kazemi, M. Rajabibazl, Malcolm K. Jones, S. Jafariaskari","doi":"10.5958/0974-0813.2018.00018.9","DOIUrl":"https://doi.org/10.5958/0974-0813.2018.00018.9","url":null,"abstract":"Echinococcus granulosus is considered as a significant parasitic infection with worldwide distribution. The present study was aimed to express the diagnostic antigen EpC1 in a recombinant system and set up an indirect ELISA for diagnosis of infected canines. Hydatid cysts were collected from liver and lungs of naturally infected sheep from slaughter houses and cDNA was synthesized from the protoscoleces. The EpC1 was expressed as recombinant protein in E. coli using pET28a as expression vector and purified by His-tag affinity chromatography. Expression of recombinant EpC1 was confirmed using SDS-PAGE followed by Western immunoblotting using T7-Tag monoclonal antibody and sera from naturally infected and experimentally challenged dogs. The diagnostic potential of the recombinant EpC1 was assessed against 22 dog sera using indirect ELISA. The sensitivity, specificity, positive and negative prediction values of the ELISA test were calculated as 100%, 93.3%, 88.8% and 100%, respectively. Recombinant EpC1 showed promising results for the diagnosis of echinococcosis in dogs.","PeriodicalId":17562,"journal":{"name":"Journal of Veterinary parasitology","volume":"68 1","pages":"85-91"},"PeriodicalIF":0.0,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74761644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-01-01DOI: 10.5958/0974-0813.2018.00004.9
C. J. Sree, P. Malakondaiah, V. C. Rayulu, V. Ramadevi, R. Pushpa
{"title":"Incidence of Fasciola in slaughtered sheep and goats in certain districts of Andhra Pradesh, India","authors":"C. J. Sree, P. Malakondaiah, V. C. Rayulu, V. Ramadevi, R. Pushpa","doi":"10.5958/0974-0813.2018.00004.9","DOIUrl":"https://doi.org/10.5958/0974-0813.2018.00004.9","url":null,"abstract":"","PeriodicalId":17562,"journal":{"name":"Journal of Veterinary parasitology","volume":"30 1","pages":"17"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87934289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-01-01DOI: 10.5958/0974-0813.2018.00005.0
C. K. Gowda, G. Puttalakshmamma, P. E. D'souza, G. Mamatha, B. M. Chandranaik
{"title":"Survey of gastrointestinal parasites of pigs slaughtered in Bengaluru","authors":"C. K. Gowda, G. Puttalakshmamma, P. E. D'souza, G. Mamatha, B. M. Chandranaik","doi":"10.5958/0974-0813.2018.00005.0","DOIUrl":"https://doi.org/10.5958/0974-0813.2018.00005.0","url":null,"abstract":"","PeriodicalId":17562,"journal":{"name":"Journal of Veterinary parasitology","volume":"46 1","pages":"23"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88703975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-01-01DOI: 10.5958/0974-0813.2018.00020.7
Sharath V. Sagar, K. Saini, Rinesh Kumar, M. Shakya, B. C. Saravanan, Subhamoy Ghosh
{"title":"Occurrence of unusually large Hyalomma anatolicum from Dewas district, Madhya Pradesh-case report","authors":"Sharath V. Sagar, K. Saini, Rinesh Kumar, M. Shakya, B. C. Saravanan, Subhamoy Ghosh","doi":"10.5958/0974-0813.2018.00020.7","DOIUrl":"https://doi.org/10.5958/0974-0813.2018.00020.7","url":null,"abstract":"","PeriodicalId":17562,"journal":{"name":"Journal of Veterinary parasitology","volume":"33 1","pages":"95"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74378453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-01-01DOI: 10.5958/0974-0813.2018.00015.3
S. Gowrishankar, B. R. Latha
{"title":"Exploiting oviposition pheromone-an innovative way to control mosquitoes","authors":"S. Gowrishankar, B. R. Latha","doi":"10.5958/0974-0813.2018.00015.3","DOIUrl":"https://doi.org/10.5958/0974-0813.2018.00015.3","url":null,"abstract":"","PeriodicalId":17562,"journal":{"name":"Journal of Veterinary parasitology","volume":"293 1","pages":"73"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79540724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-01-01DOI: 10.5958/0974-0813.2018.00016.5
S. Hemalatha, R. Edith, C. Sreekumar, S. Gomathinayagam, H. Gopi, P. Roy, M. Iyue, K. Kumanan
{"title":"Progressive light microscopic changes in chronic recurrent outbreaks of hepatic coccidiosis in domestic rabbits","authors":"S. Hemalatha, R. Edith, C. Sreekumar, S. Gomathinayagam, H. Gopi, P. Roy, M. Iyue, K. Kumanan","doi":"10.5958/0974-0813.2018.00016.5","DOIUrl":"https://doi.org/10.5958/0974-0813.2018.00016.5","url":null,"abstract":"","PeriodicalId":17562,"journal":{"name":"Journal of Veterinary parasitology","volume":"12 1","pages":"78"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86826445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-01-01DOI: 10.5958/0974-0813.2018.00014.1
Aditya Yudhana, Ratih Novita Praja, A. Supriyanto, Vivi Oktaviana
{"title":"First report of acanthocephalan infection in painted bronzeback tree snake (Dendrelaphis pictus)","authors":"Aditya Yudhana, Ratih Novita Praja, A. Supriyanto, Vivi Oktaviana","doi":"10.5958/0974-0813.2018.00014.1","DOIUrl":"https://doi.org/10.5958/0974-0813.2018.00014.1","url":null,"abstract":"","PeriodicalId":17562,"journal":{"name":"Journal of Veterinary parasitology","volume":"1 1","pages":"69"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83767255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-01-01DOI: 10.5958/0974-0813.2018.00003.7
S. Sahoo, S. Gomathinayagam, B. R. Latha, T. Senthilkumar
{"title":"Evidence of benzimidazole resistance in Haemonchus contortus of sheep in north eastern zone of Tamil Nadu.","authors":"S. Sahoo, S. Gomathinayagam, B. R. Latha, T. Senthilkumar","doi":"10.5958/0974-0813.2018.00003.7","DOIUrl":"https://doi.org/10.5958/0974-0813.2018.00003.7","url":null,"abstract":"","PeriodicalId":17562,"journal":{"name":"Journal of Veterinary parasitology","volume":"52 1","pages":"14-16"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84580922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-01-01DOI: 10.5958/0974-0813.2018.00010.4
K. Ananda, B. Pradeep, S. Satheesha, S. Dhanalakshmi
{"title":"Physaloptera sp. and Rhipicephalus sanguineus in a free range leopard","authors":"K. Ananda, B. Pradeep, S. Satheesha, S. Dhanalakshmi","doi":"10.5958/0974-0813.2018.00010.4","DOIUrl":"https://doi.org/10.5958/0974-0813.2018.00010.4","url":null,"abstract":"","PeriodicalId":17562,"journal":{"name":"Journal of Veterinary parasitology","volume":"19 1","pages":"48"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88575175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-01-01DOI: 10.5958/0974-0813.2018.00009.8
M. Shakya, M. Singh, A. Jayraw
{"title":"Myiasis in dogs in Mhow, Madhya Pradesh","authors":"M. Shakya, M. Singh, A. Jayraw","doi":"10.5958/0974-0813.2018.00009.8","DOIUrl":"https://doi.org/10.5958/0974-0813.2018.00009.8","url":null,"abstract":"","PeriodicalId":17562,"journal":{"name":"Journal of Veterinary parasitology","volume":"8 1","pages":"45"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85426579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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