Pub Date : 2023-12-21DOI: 10.1007/s00764-023-00271-y
D. Krüzselyi, P. Ott, Á. Móricz
{"title":"Exploring antioxidant potential in two basidiomycetous mushrooms using high-performance thin-layer chromatography–DPPH–videodensitometry","authors":"D. Krüzselyi, P. Ott, Á. Móricz","doi":"10.1007/s00764-023-00271-y","DOIUrl":"https://doi.org/10.1007/s00764-023-00271-y","url":null,"abstract":"","PeriodicalId":17676,"journal":{"name":"JPC – Journal of Planar Chromatography – Modern TLC","volume":"139 38","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138953329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-10DOI: 10.1007/s00764-023-00256-x
Carmen Jarne, Vicente L. Cebolla, Luis Membrado, José M. Escuín, Jesús Vela
Abstract Classic saturates, aromatics, resins, and asphaltenes (SARA) analysis has long enabled the chromatographic separation of complex hydrocarbon-containing products into these four families. This simplification often does not provide sufficient or correct information on the relationship between the composition of the products and the process variables studied. The obtention of profiles by gradient automated multiple development (AMD) is an interesting alternative to obtain extended SARA separations with increasing level of complexity for heavy petroleum products that adequately represent the entirety of the sample. An optimized 20-step gradient based on tetrahydrofuran (THF)‒dichloromethane‒ n -heptane over a total migration distance of 83 mm is proposed here to characterize all types of heavy oil products. The combination of ultraviolet (UV)–densitometry (at 201, 228, and 273 nm), fluorescence detection by intensity changes using berberine cation, together with on-plate recording of UV spectra of separated peaks and the comparison with those of standards, allow for a deep characterization of chemical families. In the apolar zone of the chromatogram saturates, naphthenes, naphteno-aromatics, alkyl-aromatics, and aromatics with different degrees of condensation are detected. The use of THF, both in the plate precleaning stage and in the first stages of the gradient, is responsible for the satisfactory resolution of the resins in the polar zone. AMD chromatographic zones can be related to peaks from hydrocarbon groups in classical SARA. The application of this gradient to different types of products allowed for the relation of the profiles of bitumen to their origin, differentiation of the resin part trapped in asphaltenes, control performance of deasphalting in hydrorefining products, and the control of the composition of base oils.
{"title":"Gradient high-performance thin-layer chromatography for characterizing complex hydrocarbon-containing products","authors":"Carmen Jarne, Vicente L. Cebolla, Luis Membrado, José M. Escuín, Jesús Vela","doi":"10.1007/s00764-023-00256-x","DOIUrl":"https://doi.org/10.1007/s00764-023-00256-x","url":null,"abstract":"Abstract Classic saturates, aromatics, resins, and asphaltenes (SARA) analysis has long enabled the chromatographic separation of complex hydrocarbon-containing products into these four families. This simplification often does not provide sufficient or correct information on the relationship between the composition of the products and the process variables studied. The obtention of profiles by gradient automated multiple development (AMD) is an interesting alternative to obtain extended SARA separations with increasing level of complexity for heavy petroleum products that adequately represent the entirety of the sample. An optimized 20-step gradient based on tetrahydrofuran (THF)‒dichloromethane‒ n -heptane over a total migration distance of 83 mm is proposed here to characterize all types of heavy oil products. The combination of ultraviolet (UV)–densitometry (at 201, 228, and 273 nm), fluorescence detection by intensity changes using berberine cation, together with on-plate recording of UV spectra of separated peaks and the comparison with those of standards, allow for a deep characterization of chemical families. In the apolar zone of the chromatogram saturates, naphthenes, naphteno-aromatics, alkyl-aromatics, and aromatics with different degrees of condensation are detected. The use of THF, both in the plate precleaning stage and in the first stages of the gradient, is responsible for the satisfactory resolution of the resins in the polar zone. AMD chromatographic zones can be related to peaks from hydrocarbon groups in classical SARA. The application of this gradient to different types of products allowed for the relation of the profiles of bitumen to their origin, differentiation of the resin part trapped in asphaltenes, control performance of deasphalting in hydrorefining products, and the control of the composition of base oils.","PeriodicalId":17676,"journal":{"name":"JPC – Journal of Planar Chromatography – Modern TLC","volume":"101 50","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135136545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-01DOI: 10.1007/s00764-023-00253-0
Simone O. Vaz, Indu Anna George
{"title":"In silico molecular docking and in vitro antimicrobial efficacy of Ficus benghalensis L. var. krishnae (C. DC.) Corner leaf extracts with high-resolution liquid chromatography and mass spectrometry phytochemical profiling","authors":"Simone O. Vaz, Indu Anna George","doi":"10.1007/s00764-023-00253-0","DOIUrl":"https://doi.org/10.1007/s00764-023-00253-0","url":null,"abstract":"","PeriodicalId":17676,"journal":{"name":"JPC – Journal of Planar Chromatography – Modern TLC","volume":"7 3","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135270995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Antioxidant activity and simultaneous estimation of four polyphenolics in different parts of Carica papaya L. by a validated high-performance thin-layer chromatography method","authors":"Vishwjeet Jadaun, Prateeksha Prateeksha, Tapan Nailwal, Brahma Nand Singh","doi":"10.1007/s00764-023-00251-2","DOIUrl":"https://doi.org/10.1007/s00764-023-00251-2","url":null,"abstract":"","PeriodicalId":17676,"journal":{"name":"JPC – Journal of Planar Chromatography – Modern TLC","volume":"154 5","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136068136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Box‒Behnken design-assisted development of an eco-friendly thin-layer chromatography‒densitometry method for the quantification of amlodipine besylate, metoprolol succinate, and telmisartan in synthetic mixture using AGREE tool","authors":"Kalpana Patel, Prachi Macwan, Anand Prajapati, Hemangini Patel, Rajesh Parmar","doi":"10.1007/s00764-023-00254-z","DOIUrl":"https://doi.org/10.1007/s00764-023-00254-z","url":null,"abstract":"","PeriodicalId":17676,"journal":{"name":"JPC – Journal of Planar Chromatography – Modern TLC","volume":"27 3","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134909049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-19DOI: 10.1007/s00764-023-00255-y
Callum-James Anton, Felix Ecker, Myriam Braun-Münker
Abstract Ibuprofen is a pharmaceutical that is commonly used to reduce inflammation and to treat headaches. A thin-layer chromatographic method combined with simple and inexpensive digital imaging is proposed for the determination and quantification of ibuprofen. The recommendations of the standard pharmacopoeia were followed with the addition of a minimal spotting method as a local calibration curve ( n = 3) and comparison with a spotted sample. Images were acquired using a smartphone and then converted to greyscale using Fiji, an open-source image analysis software. The peak area and density of each spot was then calculated using a densitometric principle. To analyse the present image data, several images were taken with different filters. Using a calibration curve ranging in the range of 3 to 5 mg/mL, linearity could be determined across the subjected images ( R 2 ranging from 0.984 to 0.996), with a mean accuracy of 99.9 ± 2.20% and RSD of 2.20%. The method studied, using smartphone imaging and open-source software (Fiji), offered a simple and inexpensive application for the quantification of ibuprofen in pharmaceuticals, applicable to preliminary studies and curricula. Graphical abstract Method proposal for ibuprofen quantification using digital image processing software (Fiji)
{"title":"Thin-layer chromatography quantification of ibuprofen using digital imaging","authors":"Callum-James Anton, Felix Ecker, Myriam Braun-Münker","doi":"10.1007/s00764-023-00255-y","DOIUrl":"https://doi.org/10.1007/s00764-023-00255-y","url":null,"abstract":"Abstract Ibuprofen is a pharmaceutical that is commonly used to reduce inflammation and to treat headaches. A thin-layer chromatographic method combined with simple and inexpensive digital imaging is proposed for the determination and quantification of ibuprofen. The recommendations of the standard pharmacopoeia were followed with the addition of a minimal spotting method as a local calibration curve ( n = 3) and comparison with a spotted sample. Images were acquired using a smartphone and then converted to greyscale using Fiji, an open-source image analysis software. The peak area and density of each spot was then calculated using a densitometric principle. To analyse the present image data, several images were taken with different filters. Using a calibration curve ranging in the range of 3 to 5 mg/mL, linearity could be determined across the subjected images ( R 2 ranging from 0.984 to 0.996), with a mean accuracy of 99.9 ± 2.20% and RSD of 2.20%. The method studied, using smartphone imaging and open-source software (Fiji), offered a simple and inexpensive application for the quantification of ibuprofen in pharmaceuticals, applicable to preliminary studies and curricula. Graphical abstract Method proposal for ibuprofen quantification using digital image processing software (Fiji)","PeriodicalId":17676,"journal":{"name":"JPC – Journal of Planar Chromatography – Modern TLC","volume":"32 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135730747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Isolation, identification, and quantification of stigmasterol in Hygrophila schulli plant by a validated high-performance thin-layer chromatography‒densitometric method","authors":"Nikita Takale, Tejaswini Kothawale, Balu Ghule, Nandkishor Kotagale","doi":"10.1007/s00764-023-00252-1","DOIUrl":"https://doi.org/10.1007/s00764-023-00252-1","url":null,"abstract":"","PeriodicalId":17676,"journal":{"name":"JPC – Journal of Planar Chromatography – Modern TLC","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136210390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-10DOI: 10.1007/s00764-023-00249-w
Hardik L. Varu, Rajesh A. Jadav, Atul H. Bapodra, Mrunal A. Ambasana
{"title":"Analytical method development, validation and studies of degradation behavior of erlotinib hydrochloride in bulk and tablet formulation by thin-layer chromatography","authors":"Hardik L. Varu, Rajesh A. Jadav, Atul H. Bapodra, Mrunal A. Ambasana","doi":"10.1007/s00764-023-00249-w","DOIUrl":"https://doi.org/10.1007/s00764-023-00249-w","url":null,"abstract":"","PeriodicalId":17676,"journal":{"name":"JPC – Journal of Planar Chromatography – Modern TLC","volume":"75 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136296045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-26DOI: 10.1007/s00764-023-00250-3
Mila Lazović, Ilija Cvijetić, Jelena Trifković, Snežana Agatonović-Kuštrin, David Morton, Petar Ristivojević
{"title":"Green analytical profiling of Teucrium chamaedrys L. using natural deep eutectic solvents and planar chromatography: a greenness assessment by the National Environmental Methods Index and analytical eco-scale","authors":"Mila Lazović, Ilija Cvijetić, Jelena Trifković, Snežana Agatonović-Kuštrin, David Morton, Petar Ristivojević","doi":"10.1007/s00764-023-00250-3","DOIUrl":"https://doi.org/10.1007/s00764-023-00250-3","url":null,"abstract":"","PeriodicalId":17676,"journal":{"name":"JPC – Journal of Planar Chromatography – Modern TLC","volume":"138 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134886732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-25DOI: 10.1007/s00764-023-00248-x
Mona T. Ragab, Nesrin K. Ramadan, Nariman A. El-Ragehy, Badr A. El-Zeany
Abstract A novel, green, and cost-effective thin-layer chromatography (TLC)‒spectrodensitometric method was designed and validated for the simultaneous determination of a five-component mixture. The analyzed mixture is composed of three active ingredients: propyphenazone (PRO), caffeine (CAF), and ergotamine tartrate (ERG), along with two official impurities which are PRO impurity: phenazone (PHN) and CAF impurity: theophylline (THEO). The suggested method was used for the quantitation of the three coformulated active ingredients in their marketed tablet and in human plasma. The studied compounds were separated on TLC silica gel 60F 254 plates using a mobile phase consisting of methanol–ethyl acetate–glacial acetic acid (1:9:0.1, V/V ) with diprophylline (DPP) as internal standard. Densitometric scanning was carried out at 210.0 nm. Method validation was assessed according to the International Council for Harmonisation (ICH) guidelines. The greenness profile for the proposed method was evaluated using the National Environmental Method Index (NEMI), analytical eco-scale, and Green Analytical Procedure Index (GAPI) tools. The proposed method offers the advantages of being simple, rapid, economic, and ecofriendly. It is a successful choice for the routine analysis of the studied drugs in pharmaceutical and biological samples.
{"title":"Thin layer chromatography‒spectrodensitometric determination of a three-component mixture of propyphenazone, caffeine, ergotamine tartrate, and two of their impurities with application to tablets, spiked human plasma, and green profile assessment","authors":"Mona T. Ragab, Nesrin K. Ramadan, Nariman A. El-Ragehy, Badr A. El-Zeany","doi":"10.1007/s00764-023-00248-x","DOIUrl":"https://doi.org/10.1007/s00764-023-00248-x","url":null,"abstract":"Abstract A novel, green, and cost-effective thin-layer chromatography (TLC)‒spectrodensitometric method was designed and validated for the simultaneous determination of a five-component mixture. The analyzed mixture is composed of three active ingredients: propyphenazone (PRO), caffeine (CAF), and ergotamine tartrate (ERG), along with two official impurities which are PRO impurity: phenazone (PHN) and CAF impurity: theophylline (THEO). The suggested method was used for the quantitation of the three coformulated active ingredients in their marketed tablet and in human plasma. The studied compounds were separated on TLC silica gel 60F 254 plates using a mobile phase consisting of methanol–ethyl acetate–glacial acetic acid (1:9:0.1, V/V ) with diprophylline (DPP) as internal standard. Densitometric scanning was carried out at 210.0 nm. Method validation was assessed according to the International Council for Harmonisation (ICH) guidelines. The greenness profile for the proposed method was evaluated using the National Environmental Method Index (NEMI), analytical eco-scale, and Green Analytical Procedure Index (GAPI) tools. The proposed method offers the advantages of being simple, rapid, economic, and ecofriendly. It is a successful choice for the routine analysis of the studied drugs in pharmaceutical and biological samples.","PeriodicalId":17676,"journal":{"name":"JPC – Journal of Planar Chromatography – Modern TLC","volume":"55 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135815760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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