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Brewing tests of new fine aroma hop varieties (Humulus lupulus L.) Saaz Brilliant, Saaz Comfort and Saaz Shine 精香啤酒花新品种酿造试验Saaz Brilliant, Saaz Comfort和Saaz Shine
Pub Date : 2021-01-01 DOI: 10.18832/kp2021.67.464
A. Mikyška, K. Štěrba, Martin Slabý, V. Nesvadba, J. Charvátová
Characterization of varietal profiles of hop secondary metabolites in beer is of considerable importance for hop growing and brewing. This three-year study focused on pilot brewing tests of three new Saaz derived hop varieties, namely Saaz Brilliant, Saaz Shine and Saaz Comfort. The sensory profiles of kettle and kettle+dry single hopped beers were monitored. At the same time, the impact of dry hopping technology on sensory perception of final beers and changes in volatile compounds was investigated. Pilot brews (200 l) of 12% pale lager were prepared in kettle and kettle+dry variants using the new Czech varieties and traditional Saaz as a control sample. Essential oils in beers were determined by GC/MS-MS method with the aim to clarify links to the beer sensory profile of both, kettle and dry hopping mode. The descriptive sensory method of hop-derived aromas and the triangle tests were employed to determine the sensory quality of beer. The overall sensory impression of beers from all tested varieties was at least comparable to traditional Saaz, and Saaz Comfort even reached better evaluation. The profile of essential oils and hop flavours in beer was partially different, however it was not rated worse than Saaz. The kettle+dry hopped beers of new varieties were significantly different from Saaz in the triangle test, Saaz Brilliant was preferred over the Saaz. The results showed considerable potential of new varieties for the production of interesting and taste-specific beers and the enrichment of the spectrum of fine aroma varieties of Czech hops.
啤酒中啤酒花次生代谢产物的品种特征研究对啤酒花的生长和酿造具有重要意义。这项为期三年的研究重点是三种新的Saaz衍生啤酒花品种的中试酿造试验,即Saaz Brilliant, Saaz Shine和Saaz Comfort。对壶啤酒和壶+干单跳啤酒的感官特征进行了监测。同时,研究了跳干工艺对成品啤酒感官知觉及挥发性物质变化的影响。以新的捷克品种和传统的萨兹作为对照样品,在水壶和水壶+干型中制备了200升12%的淡啤酒。采用气相色谱/质谱联用方法对啤酒中的精油进行了测定,目的是明确啤酒感官特征与水壶和干跳模式之间的联系。采用酒花香气描述感官法和三角试验法测定啤酒的感官质量。所有测试品种的啤酒的整体感官印象至少与传统的Saaz相当,Saaz Comfort甚至获得了更好的评价。啤酒中精油和啤酒花味道的特征部分不同,但它并不比Saaz差。在三角试验中,新品种水壶+干啤酒花啤酒与赛士啤酒有显著差异,比赛士啤酒更受青睐。结果表明,捷克啤酒花的新品种在生产有趣和有味道的啤酒方面具有相当大的潜力,并且丰富了捷克啤酒花的优良香气品种的光谱。
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引用次数: 0
Analysis and prognosis of bitter acids content in Czech hop varieties – year 2020 and long-term comparisons and trends 捷克啤酒花品种苦酸含量分析与预测- 2020年及长期比较与趋势
Pub Date : 2021-01-01 DOI: 10.18832/kp2021.67.474
Alexandr MikyĹĄka, Marie JurkovĂĄ
The article deals with the harvest forecast of the content of bitter acids in Czech hop varieties harvested in 2020. This is presented in the context of long-term trends in the content and composition of bitter acids analogues and the stability of the varietal characteristics in the aforementioned harvest. This analysis is usually available at the end of the calendar year. 180 hop samples were analysed using EBC 7.7 (HPLC) method. The α-acids content in the most important variety of Saaz (3.65% in dry matter) was 12.8% rel. higher than in the 2019 harvest and 4.2% rel. higher than the 28-year average. The α-acids content in Saaz Late (3.23%), Sladek (7.9%), Kazbek (6.4%) and Agnus (12.4%) was significantly higher than both the long-term averages for the varieties and the harvest of 2019. The Premiant (8.0%) variety was close to the long-term average. The ratio of α-/β-acids, the relative content of cohumulone and the relative content of colupulone were consistent with long-term averages of the tested varieties. It was confirmed that the harvest forecast provides timely information on the α-acids content of the given harvest, the results being in good agreement with the values of the final evaluation of all harvested hops.
本文对2020年捷克啤酒花品种苦酸含量的收获情况进行了预测。这是在苦酸类似物的含量和组成的长期趋势以及上述收获中品种特征的稳定性的背景下提出的。这种分析通常在历年结束时提供。采用ebc7.7高效液相色谱法对180份啤酒花样品进行分析。最重要品种赛阿士的α-酸含量(干物质含量3.65%)比2019年收获季高12.8%,比28年平均水平高4.2%。萨阿兹晚播(3.23%)、斯拉德克(7.9%)、哈兹别克(6.4%)和阿格努斯(12.4%)的α-酸含量均显著高于这些品种的长期平均值和2019年的收成。Premiant品种(8.0%)接近长期平均水平。α-/β-酸比值、科乌卢酮相对含量和科乌卢酮相对含量与供试品种的长期平均值一致。结果表明,收获预报能及时地反映给定收获的α-酸含量,其结果与所有收获啤酒花的最终评价值吻合良好。
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引用次数: 4
Non-microbiological turbidity of beer: Part 1 – semireview 啤酒的非微生物浊度:第1部分-半回顾
Pub Date : 2021-01-01 DOI: 10.18832/kp2021.67.484
J. Olšovská, L. Kyselová, P. Kubizniaková, Martin Slabý
Beer is a complex mixture consisting of hundreds of chemical substances. Some of them are macromolecules, such as proteins and polysaccharides that together with polyphenolic compounds form poorly soluble complexes causing beer turbidity or cold colloidal turbidity. Furthermore, beer turbidity can be caused also by procedural particles entering into beer during brewing process (filtration and stabilization aids) or by foreign particles from external environment (mechanical impurities). If turbidity, sediment or individual particles occur in filtered and stabilized beer, their origin must be determined since brilliant visual impression of the filtered beer influences an opinion of customers on a specific product. The identification of different species of turbidity using microscopic image, particle staining, enzymatic analysis or identification precursors is clearly described in this paper. The study includes pictorial documentation of various particles that may be part of beer turbidity.
啤酒是由数百种化学物质组成的复杂混合物。其中一些是大分子,如蛋白质和多糖,它们与多酚化合物一起形成难溶的复合物,引起啤酒浊度或冷胶体浊度。此外,在酿造过程中进入啤酒的程序性颗粒(过滤和稳定助剂)或来自外部环境的外来颗粒(机械杂质)也可能引起啤酒浑浊。如果在过滤和稳定的啤酒中出现浑浊,沉淀物或单个颗粒,则必须确定它们的来源,因为过滤后的啤酒的良好视觉印象会影响顾客对特定产品的看法。本文清楚地描述了利用显微图像、颗粒染色、酶分析或鉴定前体来鉴定不同种类的浊度。这项研究包括各种可能是啤酒浊度的颗粒的图片记录。
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引用次数: 1
Breeding of flavour hops in the Czech Republic 捷克共和国风味啤酒花的培育
Pub Date : 2020-12-15 DOI: 10.18832/KP2019.66.366
V. Nesvadba, J. Charvátová, S. Trnková
Hop breeding in the Czech Republic develops a number of flavour hops. The Kazbek hop variety was registered in 2008 and the Mimosa variety in 2019. The best 7 hop genotypes were selected from breeding materials between the years 2014 and 2019. Kazbek, 5512 (Ceres) and 5571 (Eris) expressed a clear citrusy flavour. Mimosa, 5495 (Juno) and 5520 (Saturn) could be described as fruity while 5164 (Uran), 5540 (Jupiter) and 5580 (Pluto) as rather spicy. Genotype 5164 showed the highest aroma intensity, content of alpha acids (11.51% w/w) and alpha/beta ratio (2.20). Whereas Mimosa demonstrated the highest content of beta acids (5.56% w/w) and the lowest alpha/beta ratio (0.28), 5571 and 5520 had the highest cohumulone amount (49.19% rel. and 47.35% rel., respectively). In contrast, 5580 were distinguished by the lowest cohumulone (21.28% rel.). The highest amount of hop oils was determined in 5164 (1.77 w/w) and 5520 (1.53 w/w). 5164, 5540 and 5495 show a myrcene level above 40% rel. On the other hand, Kazbek, Mimosa and 5571 indicated a myrcene content below 35%. The highest level of farnesene (12.50% rel.) was detected in 5164; in other samples the values were below 1% rel. Mimosa showed the highest concentration of selinenes (32.75% rel.); other samples demonstrated selinenes below 10% rel. The Kazbek variety is the foundation for the breeding of flavour hops in the Czech Republic, and therefore it is the mother plant of genotypes 5495, 5512, 5520, 5540 and 5571. Harmonie is the mother plant of genotype 5580.
捷克共和国的啤酒花育种开发了许多风味啤酒花。哈萨克啤酒花品种于2008年注册,含羞草品种于2019年注册。从2014 ~ 2019年的选育材料中筛选出7个最佳的啤酒花基因型。Kazbek, 5512(谷神星)和5571(厄里斯)表现出明显的柑橘味。含羞草,5495(朱诺)和5520(土星)可以被描述为水果,而5164(天王星),5540(木星)和5580(冥王星)是相当辛辣。基因5164表现出最高的香气强度、α酸含量(11.51% w/w)和α / β比(2.20)。含含草的β酸含量最高(5.56% w/w), α / β比最低(0.28),而5571和5520的cohumulone含量最高(分别为49.19%和47.35%)。相比之下,5580株的草葎草酮含量最低(21.28%)。5164 (1.77 w/w)和5520 (1.53 w/w)的啤酒花油含量最高。5164、5540和5495月桂烯含量在40%以上,而Kazbek、Mimosa和5571月桂烯含量在35%以下。法尼烯含量最高的年份为5164年(12.50%);含羞草中丝氨酸含量最高(32.75%);其他样品的硒含量低于10%。哈萨克品种是捷克共和国风味啤酒花育种的基础,因此它是基因型5495、5512、5520、5540和5571的母株。和谐是5580基因型的母株。
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引用次数: 4
Sensorial character of beer dry hopped with new Czech genotypes of hops with a specific flavor 新捷克啤酒花基因型干啤酒花啤酒的感官特征
Pub Date : 2020-12-15 DOI: 10.18832/KP2019.66.372
L. Strakova, V. Nesvadba, J. Křivánek, Martin Slabý, Tomáš Vrzal, J. Charvátová, R. Cerkal, J. Olšovská
In the Czech Republic breeding of new hop varieties is traditionally performed. Its properties correspond with the requirements and interests of end users, in particular hop growers, brewers and especially beer consumers, who with an increasing favor taste new types of beer. The study presents 8 new promising aromatic genotypes, whose major part (Uran, Juno, Ceres, Jupiter, Eris and Most) is already in the registration process of ÚKZÚZ of the Czech Republic (Central Institute for Supervising and Testing in Agriculture CISTA). The tested genotypes were described using brewing tests. Regarding the character of the tested hop varieties, there was chosen a uniform and simple infusion recipe with a top fermentation and dry hopping. The final beer samples were evaluated using chemical and sensorial analyses focusing on a hop aroma. All beer samples were rated as very good, the overall impression of the samples on the scale of 1 to 9 ranged from 2.9 to 3.1. The hop aroma and also bitterness character of the final beer samples were evaluated of high quality. Depending on the hop profile of the beer samples, there were suggested beer styles where the given varieties can be successfully used. These results indicate a high potential of these varieties for the use in brewing practice. Finally, the probability of perceiving a particular hop aroma in beer given by perceiving the aroma in hops was determined by Bayes’ theorem. It was found that probability particularly for spicy, resinous, herbal, citrusy, floral, fruity and grassy aroma was 100, 100, 100, 40, 38, 43, and 100%, respectively.
在捷克共和国,培育啤酒花新品种是一项传统活动。它的特性符合最终用户的要求和兴趣,特别是啤酒花种植者、酿酒师,特别是啤酒消费者,他们越来越喜欢品尝新型啤酒。该研究提出了8个新的有前景的芳香基因型,其主要部分(Uran, Juno, Ceres, Jupiter, Eris和Most)已经在捷克共和国(中央农业监督和测试研究所)的ÚKZÚZ注册过程中。用酿造试验对所测基因型进行了描述。针对所试啤酒花品种的特点,选择了一种均匀、简单、顶部发酵、干跳的浸泡配方。最后的啤酒样品是用化学和感官分析来评估的,重点是啤酒花的香气。所有的啤酒样品都被评为非常好,在1到9的范围内,样品的整体印象在2.9到3.1之间。最终样品的酒花香气和苦味特征均为优质啤酒。根据啤酒样品的啤酒花特征,有建议的啤酒风格,其中给定的品种可以成功地使用。这些结果表明这些品种在酿造实践中具有很高的潜力。最后,通过贝叶斯定理确定感知啤酒花香气给出的感知啤酒中特定啤酒花香气的概率。结果发现,辣味、树脂味、草本、柑橘味、花香、果味和草味的概率分别为100、100、100、40、38、43和100%。
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引用次数: 2
Microbiology of brewing production - bacteria of the order Enterobacterales and culture methods for their detection 酿酒生产中的微生物学。肠杆菌目细菌及其培养检测方法
Pub Date : 2020-10-15 DOI: 10.18832/kp2019.66.345
P. Kubizniaková, M. Brožová, K. Štulíková, Eva Vontrobovă, Katarína Hanzalíková, D. Matoulková
The growth of 7 strains belonging to the order of Enterobacterales, represented by the species of Citrobacter Freundii, Enterobacter cloacae, Escherichia coli, Klebsiella oxytoca, Obesumbacterium proteus, Rahnella aquatilis, Raoultella terrigena, Serratia marcescens and Shimwellia pseudoproteus, was monitored on selected cultivation media. Three types of agars - Endo, MacConkey and Chromocult Coliform agar together with two incubation temperatures of 28 and 37 °C were tested under aerobic conditions. The aim of the study was to detect such essential enterobacteria harmful to beer that cannot be proven at 37 °C, which is the temperature usually used in operational laboratories in breweries. Our results showed that most of the tested strains of enterobacteria were able to grow at 28 °C on all selected types of agar. The exception was just the representatives detection of which is problematic at 37 °C. Nevertheless, a little or no growth was always observed on just one of the tested media.
在选定的培养基上监测了弗氏柠檬酸杆菌、阴沟肠杆菌、大肠杆菌、氧化克雷伯菌、变形杆菌、水生拉赫菌、terroutella Raoultella、粘质沙雷氏菌和假变形shimwelia等7种肠杆菌目菌株的生长情况。在有氧条件下,对Endo、MacConkey和Chromocult Coliform琼脂进行了28℃和37℃两种孵育温度的测试。这项研究的目的是检测这些对啤酒有害的肠道细菌,这些细菌在37°C(啤酒厂的操作实验室通常使用的温度)下无法被证实。我们的结果表明,大多数测试的肠杆菌菌株能够在28°C下在所有选择的琼脂上生长。唯一的例外是代表在37°C下检测有问题。然而,只在一种被测介质上观察到很少或没有生长。
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引用次数: 0
Production and characterisation of non-alcoholic beer using special yeast 用特殊酵母生产无酒精啤酒及其特性
Pub Date : 2020-10-15 DOI: 10.18832/kp2019.66.336
Peter Vaštík, D. Šmogrovičová, Valentína Kafková, P. Sulo, K. Furdíková, I. Špánik
Non-Saccharomyces yeast strains Saccharomycodes ludwigii, Schizosaccharomyces pombe, Lachancea fermentati and Pichia angusta together with a hybrid yeast strain cross-bred between genetically modified Saccharomyces cerevisiae W303-1A G418R and Saccharomyces eubayanus as well as the parent yeasts of the hybrid were studied for potential use for non-alcoholic beer production. The hybrid yeast, its Saccharomyces cerevisiae W303-1A G418R parent and Saccharomycodes ludwigii were not able to metabolise maltose during Durham tube tests. Schizosaccharomyces pombe, Lachancea fermentati and Pichia angusta metabolised maltose, however, showed limited ethanol production. Parameters, volatile and non-volatile organic compounds of beers produced by the studied yeast were analysed and compared to a beer produced by bottom fermented brewer’s yeast Saccharomyces pastorianus.
研究了非酵母菌菌株ludwig酵母菌、pombe Schizosaccharomyces、Lachancea fermentati和Pichia angusta,以及转基因酿酒酵母菌W303-1A G418R与真bayanus酵母菌杂交的杂交酵母菌及其亲本酵母在无酒精啤酒生产中的潜在应用。在达勒姆试管试验中,杂交酵母、其酿酒酵母W303-1A G418R亲本和路德维希酵母不能代谢麦芽糖。裂糖菌pombe、发酵拉香菌和毕赤酵母代谢麦芽糖,但乙醇产量有限。对所研究的酵母生产的啤酒的参数、挥发性和非挥发性有机化合物进行了分析,并与底部发酵的酿酒酵母生产的啤酒进行了比较。
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引用次数: 5
Development of a method for melamine determination in beer and beer-type beverages by GC-MS/MS 气相色谱-质谱联用法测定啤酒及啤酒类饮料中的三聚氰胺
Pub Date : 2020-10-15 DOI: 10.18832/kp2019.66.331
M. Malečková, Tomáš Vrzal, J. Olšovská
Based on the European regulation, the Czech Association of Breweries and Malthouses recommends 2.5 mg/kg as maximum permitted concentration of melamine for beer, barley, malt, hop, and their products. For this reason, the first analytical method for melamine determination in beer and beer-type beverages was developed. The sample preparation included ion-exchange solid-phase extraction and subsequent derivatization with BSTFA. Qualitative and quantitative analysis was performed by gas chromatography with tandem mass spectrometry. Validation parameters of the method, such as limits of detection and quantification were 0.1 mg/L and 0.4 mg/L, respectively. Extended uncertainty of the final method was evaluated to 0.1 mg/L. The method was applied on twenty samples of beer lagers, or other beer styles; all had the level of melamine under the limit of quantification (< 0.4 mg/L).
根据欧洲的规定,捷克啤酒厂和麦芽协会建议啤酒、大麦、麦芽、啤酒花及其产品的三聚氰胺最高允许浓度为2.5毫克/公斤。为此,首次建立了啤酒及啤酒类饮料中三聚氰胺的测定方法。样品制备包括离子交换固相萃取和BSTFA衍生化。采用气相色谱串联质谱法进行定性和定量分析。方法的检出限和定量限分别为0.1 mg/L和0.4 mg/L。最终方法的扩展不确定度评定为0.1 mg/L。该方法应用于20个啤酒窖藏或其他啤酒类型的样品;三聚氰胺含量均低于定量限(< 0.4 mg/L)。
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引用次数: 1
Balling's attenuation theory and beer composition calculator 球化衰减理论及啤酒成分计算器
Pub Date : 2020-10-15 DOI: 10.18832/kp2019.66.351
Vilém Nohel
This article deals with calculation possibilities and the control of basic parameters of beer on the basis of knowledge of two parameters. The importance of this method increases with the development of craft beer production in our country (mini-breweries, home brewing). The calculations are based on Balling's equations, alcohol factors and attenuation quotients, which have been published unchanged since at least 1876. In Balling's time, they were used to determine an extract with a specific gravities of 17.5 °C/17.5 °C. In current brewing, specific gravities of 20 °C/20 °C are used. For this reason, new alcohol factors of apparent attenuation were derived, and based on linear regression, regression coefficients of the dependence of the alcohol factor of apparent attenuation on the extract in original wort were calculated. Some variants of calculations based on two input parameters were incorporated into the beer composition calculator using formulas that replace extract and alcohol tables. The accuracy of the results from the calculator was checked with analyses from the Anton Paar automatic analyzer and with analyses obtained by the distillation method. The calculator is available at www.balling.cz.
本文在了解这两个参数的基础上,讨论了啤酒基本参数的计算可能性和控制问题。随着我国精酿啤酒生产(小型啤酒厂、家庭酿造)的发展,这种方法的重要性与日俱增。这些计算是基于巴灵方程、酒精因子和衰减商,这些公式至少自1876年以来一直没有改变过。在Balling的时代,他们被用来确定一个萃取物的比重为17.5°C/17.5°C。在当前酿造中,比重为20°C/20°C。为此,导出了新的表观衰减酒精因子,并在线性回归的基础上,计算了表观衰减酒精因子对原麦汁中提取物依赖程度的回归系数。基于两个输入参数的一些计算变体被纳入啤酒成分计算器,使用公式取代提取物和酒精表。计算结果的准确性与安东帕自动分析仪的分析结果和蒸馏法的分析结果进行了验证。该计算器可在www.balling.cz上获得。
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引用次数: 0
Fortification of Beer with Extracts of the Selected Czech Medicinal Herbs and Plants 精选捷克草药和植物提取物强化啤酒
Pub Date : 2020-08-13 DOI: 10.18832/kp2019.66.314
Helena Pluháčková, T. Gregor, R. Boško, S. Běláková, Z. Svoboda, K. Benešová
This study investigated the possibilities of fortification of beer with various herbal extracts. The aim was to fortify beer with natural substances that have favourable effects on consumers ́ health. The tested beers were enriched with ethanol extracts of medicinal herbs (ribwort plantain, small leaved linden, purple coneflower, chamomile, sage) so that the sensory characters of the supplemented herbal extract created only an accompanying perception and the malt and hop flavour of beer was preserved. In the enriched beers, the contents of the selected phenolic substances and important essential oils were determined and sensory evaluation was performed. The content of phenolic substances increased the most in beer with linden (42.46 mg/l) and plantain (37.5 mg/l) extracts, approximately four times compared to the original beer (8.11 mg/l). Beer with sage and chamomile extract achieved the best sensory assessment, with components of essential oils added in units of ng/l of beer.
本研究探讨了用各种草药提取物强化啤酒的可能性。其目的是用对消费者健康有益的天然物质来强化啤酒。在测试的啤酒中加入草药的乙醇提取物(车前草、小叶椴、紫芋花、洋甘菊、鼠尾草),这样补充的草药提取物的感官特征只产生一种伴随的感觉,啤酒的麦芽和啤酒花风味被保留了下来。对浓缩啤酒中所选酚类物质和重要精油的含量进行测定,并进行感官评价。以菩提树(42.46 mg/l)和车前草(37.5 mg/l)提取物为原料的啤酒中酚类物质含量增加最多,约为原啤酒(8.11 mg/l)的4倍。添加鼠尾草和洋甘菊提取物的啤酒获得了最好的感官评价,以ng/l啤酒为单位添加了精油成分。
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引用次数: 7
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Kvasny prumysl
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