Pub Date : 2021-06-30DOI: 10.7841/ksbbj.2021.36.2.154
Hyun-Jin Jang, Soon-Ah Lee, Eun-Jin Hong, Z. Kim, K. J. Yim, B. Jo, S. Nam, Y. Nam, B. Hwang, C. Lee, J. Jung, Sung Hwan Woo
{"title":"Antioxidant and Anticancer Activities of Methanolic Extracts from Indigenous Freshwater Green Microalgae","authors":"Hyun-Jin Jang, Soon-Ah Lee, Eun-Jin Hong, Z. Kim, K. J. Yim, B. Jo, S. Nam, Y. Nam, B. Hwang, C. Lee, J. Jung, Sung Hwan Woo","doi":"10.7841/ksbbj.2021.36.2.154","DOIUrl":"https://doi.org/10.7841/ksbbj.2021.36.2.154","url":null,"abstract":"","PeriodicalId":17865,"journal":{"name":"KSBB Journal","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75954889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-03-31DOI: 10.7841/KSBBJ.2021.36.1.42
Kyung-Mi Lee, Taejin Park, E. Jie, Sukweon Kim, Dong-Hwan Han, Seung-Young Kim
{"title":"Melanogenesis Inhibitory Effects of Phryma leptostachya Callus using Biorenovation in B16F10 Melanoma Cells","authors":"Kyung-Mi Lee, Taejin Park, E. Jie, Sukweon Kim, Dong-Hwan Han, Seung-Young Kim","doi":"10.7841/KSBBJ.2021.36.1.42","DOIUrl":"https://doi.org/10.7841/KSBBJ.2021.36.1.42","url":null,"abstract":"","PeriodicalId":17865,"journal":{"name":"KSBB Journal","volume":"36 1","pages":"42-48"},"PeriodicalIF":0.0,"publicationDate":"2021-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87697585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-03-31DOI: 10.7841/KSBBJ.2021.36.1.68
So Yeon Jeong, Se-A Lee, N. Gu, Seong-In Lim, Jienny Lee, Yoon-Hee Lee, B. Hyun
Mesenchymal stem cells (MSCs) exhibit self-renewing capacity and multi-lineage differentiation potential. CD271 has been identified as a marker of the most homogeneous MSCs subset. In this study, we separated CD271 positive MSCs (CD271-MSCs) from canine adipose tissue-derived MSCs (AD-MSCs) using immunomagnetic selection method. Both CD271-MSCs and AD-MSCs showed similar morphology and expressed common immunophenotypic markers (CD34, CD44, and CD45). However, CD271-MSCs and AD-MSCs exhibited difference in chondrogenic differentiation potential. Specifically, CD271-MSCs exhibited increased expression levels of chondrocyte specific markers, collagen type II alpha 1 (COL2A1) and Aggrecan compared to AD-MSCs. Also, electrical stimulation (ES)-treated CD271-MSCs have a greater capacity for expressing heat shock protein 70 (HSP70), COL2A1, and Aggrecan compared to control without exogenous differentiation condition. This study might contribute to the differentiation potential of homogenous MSCs into cartilage cells by specific ES condition in the absence of exogenous differentiation agents.
{"title":"Chondrogenic Differentiation Potential of CD271 Positive Mesenchymal Stem Cells through Electrical Stimulation","authors":"So Yeon Jeong, Se-A Lee, N. Gu, Seong-In Lim, Jienny Lee, Yoon-Hee Lee, B. Hyun","doi":"10.7841/KSBBJ.2021.36.1.68","DOIUrl":"https://doi.org/10.7841/KSBBJ.2021.36.1.68","url":null,"abstract":"Mesenchymal stem cells (MSCs) exhibit self-renewing capacity and multi-lineage differentiation potential. CD271 has been identified as a marker of the most homogeneous MSCs subset. In this study, we separated CD271 positive MSCs (CD271-MSCs) from canine adipose tissue-derived MSCs (AD-MSCs) using immunomagnetic selection method. Both CD271-MSCs and AD-MSCs showed similar morphology and expressed common immunophenotypic markers (CD34, CD44, and CD45). However, CD271-MSCs and AD-MSCs exhibited difference in chondrogenic differentiation potential. Specifically, CD271-MSCs exhibited increased expression levels of chondrocyte specific markers, collagen type II alpha 1 (COL2A1) and Aggrecan compared to AD-MSCs. Also, electrical stimulation (ES)-treated CD271-MSCs have a greater capacity for expressing heat shock protein 70 (HSP70), COL2A1, and Aggrecan compared to control without exogenous differentiation condition. This study might contribute to the differentiation potential of homogenous MSCs into cartilage cells by specific ES condition in the absence of exogenous differentiation agents.","PeriodicalId":17865,"journal":{"name":"KSBB Journal","volume":"4 1","pages":"68-75"},"PeriodicalIF":0.0,"publicationDate":"2021-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85514647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-03-31DOI: 10.7841/KSBBJ.2021.36.1.23
Songhee Park
{"title":"COVID-19 Diagnostic Techniques: Analysis of Nucleic Acid-based testing of SARS-CoV-2 in Accordance with Primers","authors":"Songhee Park","doi":"10.7841/KSBBJ.2021.36.1.23","DOIUrl":"https://doi.org/10.7841/KSBBJ.2021.36.1.23","url":null,"abstract":"","PeriodicalId":17865,"journal":{"name":"KSBB Journal","volume":"8 1","pages":"23-29"},"PeriodicalIF":0.0,"publicationDate":"2021-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87168218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-03-31DOI: 10.7841/KSBBJ.2021.36.1.49
Hye-Jin Han, Ji-Hee Lee, Hyun-Myoung Cha, Jin-Hyuk Lim, Dong-Il Kim
{"title":"Enhanced Sialylation of Albumin-erythropoietin through Increased Synthesis of UDP-N-acetylglucosamine in CHO Cell Cultures","authors":"Hye-Jin Han, Ji-Hee Lee, Hyun-Myoung Cha, Jin-Hyuk Lim, Dong-Il Kim","doi":"10.7841/KSBBJ.2021.36.1.49","DOIUrl":"https://doi.org/10.7841/KSBBJ.2021.36.1.49","url":null,"abstract":"","PeriodicalId":17865,"journal":{"name":"KSBB Journal","volume":"31 1","pages":"49-59"},"PeriodicalIF":0.0,"publicationDate":"2021-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90377004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-03-31DOI: 10.7841/KSBBJ.2021.36.1.76
Moon-Seop Choi, Ki-Seung Choi, K. Oh
We have characterized a novel bacterium, strain CK-4 isolated from industrial wastewater, which is capable of utilizing 1,4-dioxane as sole carbon source and energy. Complete depletion of 1 g/L 1,4-dioxane by CK-4 cultures growing on AMS media was achieved within 168 h. Both the Biolog system and phylogenetic analysis using 16S rRNA sequencing were utilized for identification, and the strain was designated as Shinella granuli CK-4, and registered in GenBank as [MN841914]. The mortality of CK-4 was not greatly affected by 1,4-dioxane concentrations below 10 g/L but was increased with increasing 1,4-dioxane concentrations. SDS-PAGE revealed that the amount of lipopolysaccharide in CK-4 increased or decreased according to the 1,4-dioxane concentration. The expression of 60-kDa GroEL stress shock protein (SSP) was increased in proportion to concentrations below 10 g/L 1,4dioxane but was not expressed at concentrations above 15 g/L dioxane. On the other hand, 70-kDa DnaK SSP was not expressed at any 1,4-dioxane concentrations (0~20 g/mL) tested. SEM demonstrated that the surface of CK-4 cells exposed to 10 g/L dioxane was not significantly different from that of normal cells, whereas cells exposed to 20 g/L 1,4-dioxane showed the presence of perforations and irregular rod forms with wrinkled cell surfaces. These results provide clues to understanding the resistance of S. granuli CK-4 to 1,4-dioxane and the promotion of 1,4-dioxane degradation, which can be applied to the treatment of industrial wastewater with higher concentrations of 1,4-dioxane. Shinella granuli CK-4, 1,4-Dioxane, biodegradation, industrial wastewater
{"title":"Characterization of Shinella granuli CK-4, a Novel Bacterium Isolated from Industrial Wastewater Capable of Degrading 1,4-Dioxane","authors":"Moon-Seop Choi, Ki-Seung Choi, K. Oh","doi":"10.7841/KSBBJ.2021.36.1.76","DOIUrl":"https://doi.org/10.7841/KSBBJ.2021.36.1.76","url":null,"abstract":"We have characterized a novel bacterium, strain CK-4 isolated from industrial wastewater, which is capable of utilizing 1,4-dioxane as sole carbon source and energy. Complete depletion of 1 g/L 1,4-dioxane by CK-4 cultures growing on AMS media was achieved within 168 h. Both the Biolog system and phylogenetic analysis using 16S rRNA sequencing were utilized for identification, and the strain was designated as Shinella granuli CK-4, and registered in GenBank as [MN841914]. The mortality of CK-4 was not greatly affected by 1,4-dioxane concentrations below 10 g/L but was increased with increasing 1,4-dioxane concentrations. SDS-PAGE revealed that the amount of lipopolysaccharide in CK-4 increased or decreased according to the 1,4-dioxane concentration. The expression of 60-kDa GroEL stress shock protein (SSP) was increased in proportion to concentrations below 10 g/L 1,4dioxane but was not expressed at concentrations above 15 g/L dioxane. On the other hand, 70-kDa DnaK SSP was not expressed at any 1,4-dioxane concentrations (0~20 g/mL) tested. SEM demonstrated that the surface of CK-4 cells exposed to 10 g/L dioxane was not significantly different from that of normal cells, whereas cells exposed to 20 g/L 1,4-dioxane showed the presence of perforations and irregular rod forms with wrinkled cell surfaces. These results provide clues to understanding the resistance of S. granuli CK-4 to 1,4-dioxane and the promotion of 1,4-dioxane degradation, which can be applied to the treatment of industrial wastewater with higher concentrations of 1,4-dioxane. Shinella granuli CK-4, 1,4-Dioxane, biodegradation, industrial wastewater","PeriodicalId":17865,"journal":{"name":"KSBB Journal","volume":"86 1","pages":"76-85"},"PeriodicalIF":0.0,"publicationDate":"2021-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73458764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-03-31DOI: 10.7841/KSBBJ.2021.36.1.36
Byeongha Choi, Taejin Park, Jung Hwan Kim, Seung-Young Kim
Brassica napus (BN) has been reported to have various drug efficacies such as cancer, aging, but there is research on the scientific efficacy of whitening effect. Biorenovation is a method of modifying the structure of a broad range of substrates such as chemical compounds and plant extract by microbial enzymes with the potential benefits of reduced cytotoxicities and enhanced biological activities relative to its parent substrates. we generated BN extract using biorenovation technology and tested the whitening effect properties of its biorenovation product (BNBR) in α-MSH stimulated B16F10 melanoma cells. Our results showed that BNBR significantly inhibited enzymes of melanogenesis such as TRP-1, TRP-2, MITF, tyrosinase in a concentration dependent manner without no noticeable toxicities. Therefore, it suggests that maybe various components that converted by biorenovation contained in the BNBR affect multiple factors involved in α-MSH-induced melanogenesis in B16F10 cells. And our results indicate that BNBR could be a new source for the ingredients of functional cosmetics.
{"title":"The Effect of Brassica napus Biorenovated Extract on the Production of Melanin Production and Tyrosinase Activity in B16F10 Melanoma Cells","authors":"Byeongha Choi, Taejin Park, Jung Hwan Kim, Seung-Young Kim","doi":"10.7841/KSBBJ.2021.36.1.36","DOIUrl":"https://doi.org/10.7841/KSBBJ.2021.36.1.36","url":null,"abstract":"Brassica napus (BN) has been reported to have various drug efficacies such as cancer, aging, but there is research on the scientific efficacy of whitening effect. Biorenovation is a method of modifying the structure of a broad range of substrates such as chemical compounds and plant extract by microbial enzymes with the potential benefits of reduced cytotoxicities and enhanced biological activities relative to its parent substrates. we generated BN extract using biorenovation technology and tested the whitening effect properties of its biorenovation product (BNBR) in α-MSH stimulated B16F10 melanoma cells. Our results showed that BNBR significantly inhibited enzymes of melanogenesis such as TRP-1, TRP-2, MITF, tyrosinase in a concentration dependent manner without no noticeable toxicities. Therefore, it suggests that maybe various components that converted by biorenovation contained in the BNBR affect multiple factors involved in α-MSH-induced melanogenesis in B16F10 cells. And our results indicate that BNBR could be a new source for the ingredients of functional cosmetics.","PeriodicalId":17865,"journal":{"name":"KSBB Journal","volume":"34 1","pages":"36-41"},"PeriodicalIF":0.0,"publicationDate":"2021-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82028226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-03-31DOI: 10.7841/KSBBJ.2021.36.1.1
Sang-Uk Kim, Gyunghyun Choi
{"title":"Technology Opportunity Discovery Based on M&A Transactions Data in Healthcare Industry","authors":"Sang-Uk Kim, Gyunghyun Choi","doi":"10.7841/KSBBJ.2021.36.1.1","DOIUrl":"https://doi.org/10.7841/KSBBJ.2021.36.1.1","url":null,"abstract":"","PeriodicalId":17865,"journal":{"name":"KSBB Journal","volume":"10 1","pages":"1-15"},"PeriodicalIF":0.0,"publicationDate":"2021-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74472977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-03-31DOI: 10.7841/KSBBJ.2021.36.1.60
S. Adhikari, Kyoung-ran Moon, Wonjae Lee
The present study aimed to evaluate the antidiarrheal and superoxide scavenging activities of ethanolic extract of Stenochlaena palustris, an edible fern from Blechnaceae family. The antidiarrheal activity was determined against castor oil induced diarrhea model, gastrointestinal transit and enteropooling tests in Swiss albino mice. The test groups received various doses (100, 200, and 400 mg/kg, body weight) of the extract, whereas positive control received loperamide (3 mg/kg, b.w.) and negative control was supplied with 1% tween-80 in distilled water (10 mL/kg, b.w.). At three test doses (100, 200 and 400 mg/kg), the extract showed significant (p < 0.001) and dose dependent antidiarrheal activity in all models. The delay and reduction in the onset, frequency, and weight of diarrheic feces as well as inhibition of the gastrointestinal transit and intraluminal fluid accumulation were observed in all models and highly comparable to the standard loperamide. The extract also exhibited high inhibitory ability to scavenge superoxide anion (O2 • ). The highest percentage inhibition of superoxide anion was found to be 84.32 ± 0.15% at the concentration 800 μg/mL.
{"title":"Assessment of Antidiarrheal and Superoxide Scavenging Activities of Ethanolic Frond Extract from Stenochlaena palustris Bedd.","authors":"S. Adhikari, Kyoung-ran Moon, Wonjae Lee","doi":"10.7841/KSBBJ.2021.36.1.60","DOIUrl":"https://doi.org/10.7841/KSBBJ.2021.36.1.60","url":null,"abstract":"The present study aimed to evaluate the antidiarrheal and superoxide scavenging activities of ethanolic extract of Stenochlaena palustris, an edible fern from Blechnaceae family. The antidiarrheal activity was determined against castor oil induced diarrhea model, gastrointestinal transit and enteropooling tests in Swiss albino mice. The test groups received various doses (100, 200, and 400 mg/kg, body weight) of the extract, whereas positive control received loperamide (3 mg/kg, b.w.) and negative control was supplied with 1% tween-80 in distilled water (10 mL/kg, b.w.). At three test doses (100, 200 and 400 mg/kg), the extract showed significant (p < 0.001) and dose dependent antidiarrheal activity in all models. The delay and reduction in the onset, frequency, and weight of diarrheic feces as well as inhibition of the gastrointestinal transit and intraluminal fluid accumulation were observed in all models and highly comparable to the standard loperamide. The extract also exhibited high inhibitory ability to scavenge superoxide anion (O2 • ). The highest percentage inhibition of superoxide anion was found to be 84.32 ± 0.15% at the concentration 800 μg/mL.","PeriodicalId":17865,"journal":{"name":"KSBB Journal","volume":"1 1","pages":"60-67"},"PeriodicalIF":0.0,"publicationDate":"2021-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88719048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-03-31DOI: 10.7841/KSBBJ.2021.36.1.30
J. Sim, Kyung-Mi Lee, Taejin Park, Minseok Kang, Hyehyun Hong, Seung-Young Kim
Biorenovation is a microbial enzyme-based structural modification of component compounds in natural products and synthetic compounds including plant crude-extracts with the potential benefits of reduced cytotoxicities and improved biological activities compared with its reaction substrates. In this study, we examined the whitening effect of Ligustrum japonicum (LJ) and LJ biorenovation extract (LJBR) in α-MSH-stimulated B16F10 melanoma cells. In the non-toxic concentrations of 25, 50, and 100 μg/ml, the inhibitory effect of LJ and LJBR on melanin synthesis and tyrosinase activity was observed. LJBR exhibited a stronger inhibitory effect on melanin synthesis and tyrosinase activity relative to its reaction substrate LJ. In addition, the protein expression level of MITF, TRP-1, TRP-2, and tyrosinase, which are known to play a critical role in melanin synthesis, was measured. As expected, a greater inhibitory effect on melanogenesis was observed in LJBR than in untreated group. These findings strongly suggest that biorenovation can assist the generation of a novel skin-lightening material.
{"title":"Biorenovation-Assisted Modifiction of Ligustrum japonicum Extract for Skin-Whitening Effect","authors":"J. Sim, Kyung-Mi Lee, Taejin Park, Minseok Kang, Hyehyun Hong, Seung-Young Kim","doi":"10.7841/KSBBJ.2021.36.1.30","DOIUrl":"https://doi.org/10.7841/KSBBJ.2021.36.1.30","url":null,"abstract":"Biorenovation is a microbial enzyme-based structural modification of component compounds in natural products and synthetic compounds including plant crude-extracts with the potential benefits of reduced cytotoxicities and improved biological activities compared with its reaction substrates. In this study, we examined the whitening effect of Ligustrum japonicum (LJ) and LJ biorenovation extract (LJBR) in α-MSH-stimulated B16F10 melanoma cells. In the non-toxic concentrations of 25, 50, and 100 μg/ml, the inhibitory effect of LJ and LJBR on melanin synthesis and tyrosinase activity was observed. LJBR exhibited a stronger inhibitory effect on melanin synthesis and tyrosinase activity relative to its reaction substrate LJ. In addition, the protein expression level of MITF, TRP-1, TRP-2, and tyrosinase, which are known to play a critical role in melanin synthesis, was measured. As expected, a greater inhibitory effect on melanogenesis was observed in LJBR than in untreated group. These findings strongly suggest that biorenovation can assist the generation of a novel skin-lightening material.","PeriodicalId":17865,"journal":{"name":"KSBB Journal","volume":"18 1","pages":"30-35"},"PeriodicalIF":0.0,"publicationDate":"2021-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90738908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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