Pub Date : 2019-04-17DOI: 10.33785/IJDS.2019.V72I02.009
Asit Jain, D. K. Barwa, Mohan B. Singh, K. Mukherjee, T. Jain, Tantia, K. Rajam, Arjava Sharma
MS Tantia, KN Raja, Arjava Sharma ICAR-NBAGR, 139, Sector 13, Urban estate, Karnal, Haryana, India Abstract: Present study was conducted to record reproductive and productive performances of Kosali cattle maintained under farmer’s management conditions in Central Plain Region of Chhattisgarh state. 345 livestock owners were randomly selected and interviewed with structured questionnaire to obtain information on reproductive performance of Kosali cattle. Average age at first calving, calving interval, service period and number of services per conception were found 54.64±2.18 months, 430.26±6.33 days, 159.59±2.67 days and 1.4 ±0.08, respectively. In male, age at first ejaculation and age at first mating were found to be 41.54±1.14 and 51.3±1.05 months, respectively. Production was recorded and average daily milk yield, peak yield, lactation milk yield, lactation length and dry period were estimated to be 0.92±0.23 kg, 1.27±0.15 kg, 210.3±4.19 kg, 230.7±9.11 days and 190.8±8.19 days, respectively. Average fat and SNF percent were 4.4±0.27 and 10.41±0.21%, respectively. Observed indices showed poor performances which are below the national average. Hence, suitable breeding strategies and training for livestock keepers and farmers on managemental aspects of Kosali cattle is needed to improve production and reproduction performances.
{"title":"Reproductive and productive performances of Kosali cattle in its native\u0000environment","authors":"Asit Jain, D. K. Barwa, Mohan B. Singh, K. Mukherjee, T. Jain, Tantia, K. Rajam, Arjava Sharma","doi":"10.33785/IJDS.2019.V72I02.009","DOIUrl":"https://doi.org/10.33785/IJDS.2019.V72I02.009","url":null,"abstract":"MS Tantia, KN Raja, Arjava Sharma ICAR-NBAGR, 139, Sector 13, Urban estate, Karnal, Haryana, India Abstract: Present study was conducted to record reproductive and productive performances of Kosali cattle maintained under farmer’s management conditions in Central Plain Region of Chhattisgarh state. 345 livestock owners were randomly selected and interviewed with structured questionnaire to obtain information on reproductive performance of Kosali cattle. Average age at first calving, calving interval, service period and number of services per conception were found 54.64±2.18 months, 430.26±6.33 days, 159.59±2.67 days and 1.4 ±0.08, respectively. In male, age at first ejaculation and age at first mating were found to be 41.54±1.14 and 51.3±1.05 months, respectively. Production was recorded and average daily milk yield, peak yield, lactation milk yield, lactation length and dry period were estimated to be 0.92±0.23 kg, 1.27±0.15 kg, 210.3±4.19 kg, 230.7±9.11 days and 190.8±8.19 days, respectively. Average fat and SNF percent were 4.4±0.27 and 10.41±0.21%, respectively. Observed indices showed poor performances which are below the national average. Hence, suitable breeding strategies and training for livestock keepers and farmers on managemental aspects of Kosali cattle is needed to improve production and reproduction performances.","PeriodicalId":179606,"journal":{"name":"Vol 72, No 2 (2019)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126002091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-04-17DOI: 10.33785/IJDS.2019.V72I02.012
Ashok Prajapati, A. Dhami, K. Hadiya, J. Patel, J. Prajapati
A study was carried out under field conditions on 50 acyclic/anestrus and 50 cyclic/ repeat breeder HF crossbred cows to evaluate the efficacy of four standard estrus induction/ ovulation synchronization protocols, viz., Ov_synch (GPG), Ov_synch Plus (PMSG+GPG), Double_synch (P-GPG) and Estradoublesynch (P-GPE) (10 cows in each protocol, and in untreated controls) in terms of estrus induction response and conception rates at induced estrus with fixed time AI (FTAI) and overall of 3 cycles. All the selected animals received presynchronization treatment, viz., Inj. Ivermectin s.c. and Tonovita i.m. once and multimineral bolus once daily for 7 days. Repeat breeding cows received additional single shot i.m. injection of Enrofloxacin. All the anestrus and repeat breeder cows (100 %) under all four treatment protocols exhibited induced estrus between 48 and 72 hrs from PGF 2 α injection. The conception rates obtained in anestrus cows at induced estrus (FTAI) and overall of three cycles post-treatment were 20.0 and 60.0% with Ov_synch; 20.0 and 50.0% with Ov_synch Plus; 40.0 and 80.0% with Double_synch, and 30.0 and 60.0% with Estradoublesynch protocol, respectively. The corresponding conception rates in repeat breeder cows were 30.0 and 70.0% with Ov_synch; 30.0 and 50.0% with Ov_synch Plus; 40.0 and 80.0% with Double_synch, and 20.0 and 60.0% with Estradoublesynch protocol, respectively. All non-conceived repeat breeding cows under each protocol, except 1 in Ov_synch Plus protocol, continued cycling, while among 10 anestrus cows each treated with Ov_synch, Ov_synch Plus, Double_synch and Estradoublesynch protocol, 2, 2, 0 and 1 cows again turned out to be anestrus by 60 days post-induction/FTAI, respectively. Among the untreated control anestrus and repeat breeder cows (10 each), 2 and 3 cows conceived over a period of 90 days follow up giving overall conception rates of only 20.0 and 30.0%, respectively, as against the overall fertility rate of 62.5 and 65.0 % in treated anestrus and repeat breeder cows, respectively, over just mean 28-32 days of treatment, suggesting the economic utility of these protocols in handling the problem breeder cows under field conditions.
{"title":"Efficacy of different estrus synchronization protocols on estrus induction\u0000response and conception rate in acyclic and cyclic crossbred cows","authors":"Ashok Prajapati, A. Dhami, K. Hadiya, J. Patel, J. Prajapati","doi":"10.33785/IJDS.2019.V72I02.012","DOIUrl":"https://doi.org/10.33785/IJDS.2019.V72I02.012","url":null,"abstract":"A study was carried out under field conditions on 50 acyclic/anestrus and 50 cyclic/ repeat breeder HF crossbred cows to evaluate the efficacy of four standard estrus induction/ ovulation synchronization protocols, viz., Ov_synch (GPG), Ov_synch Plus (PMSG+GPG), Double_synch (P-GPG) and Estradoublesynch (P-GPE) (10 cows in each protocol, and in untreated controls) in terms of estrus induction response and conception rates at induced estrus with fixed time AI (FTAI) and overall of 3 cycles. All the selected animals received presynchronization treatment, viz., Inj. Ivermectin s.c. and Tonovita i.m. once and multimineral bolus once daily for 7 days. Repeat breeding cows received additional single shot i.m. injection of Enrofloxacin. All the anestrus and repeat breeder cows (100 %) under all four treatment protocols exhibited induced estrus between 48 and 72 hrs from PGF 2 α injection. The conception rates obtained in anestrus cows at induced estrus (FTAI) and overall of three cycles post-treatment were 20.0 and 60.0% with Ov_synch; 20.0 and 50.0% with Ov_synch Plus; 40.0 and 80.0% with Double_synch, and 30.0 and 60.0% with Estradoublesynch protocol, respectively. The corresponding conception rates in repeat breeder cows were 30.0 and 70.0% with Ov_synch; 30.0 and 50.0% with Ov_synch Plus; 40.0 and 80.0% with Double_synch, and 20.0 and 60.0% with Estradoublesynch protocol, respectively. All non-conceived repeat breeding cows under each protocol, except 1 in Ov_synch Plus protocol, continued cycling, while among 10 anestrus cows each treated with Ov_synch, Ov_synch Plus, Double_synch and Estradoublesynch protocol, 2, 2, 0 and 1 cows again turned out to be anestrus by 60 days post-induction/FTAI, respectively. Among the untreated control anestrus and repeat breeder cows (10 each), 2 and 3 cows conceived over a period of 90 days follow up giving overall conception rates of only 20.0 and 30.0%, respectively, as against the overall fertility rate of 62.5 and 65.0 % in treated anestrus and repeat breeder cows, respectively, over just mean 28-32 days of treatment, suggesting the economic utility of these protocols in handling the problem breeder cows under field conditions.","PeriodicalId":179606,"journal":{"name":"Vol 72, No 2 (2019)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134272154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-04-17DOI: 10.33785/IJDS.2019.V72I02.014
D. Patel, K. Ponnusamy
Reproductive problems in dairy animals emerged as prime concern for farmers due to their significant effect on the profitability and sustainability of dairy ventures in India. The present study was undertaken to assess the major reproductive problems of dairy animals as well as to develop and validate the extension strategies for managing reproductive problems. A total of 60 respondents from three villages viz Dilawara, Chundipur and Dhakwada of Karnal district were selected through simple random sampling. Data were gathered through structured interview schedule from dairy farmers who had at least one milch animal. Study revealed that repeat breeding, anestrous, retention of fetal membrane and prolapse were the major reproductive problems of dairy animals as perceived by 46.67, 36.67, 28.33 and 26.67 per cent of respondents respectively. Among 14 selected extension strategies, development and demonstration of video on specific reproductive issues with Rank Based Quotient (RBQ) value of 81.19 and preparation of extension literature with RBQ value of 72.38 were found to be the two best strategies for faster dissemination of information on management of reproductive problems. The mean knowledge gain was found to be 10.75 per cent, 13.28 per cent and 15.23 per cent after exposure to different treatments i.e. booklet, video and both (video+booklet) respectively in the study villages. This explains the effectiveness of extension strategies and warrants the extension personnel to contextualise and deploy appropriate combinations of different extension strategies in order to improve the information delivery system.
{"title":"Development and validation of extension strategies for effective management of reproductive problems of dairy animals","authors":"D. Patel, K. Ponnusamy","doi":"10.33785/IJDS.2019.V72I02.014","DOIUrl":"https://doi.org/10.33785/IJDS.2019.V72I02.014","url":null,"abstract":"Reproductive problems in dairy animals emerged as prime concern for farmers due to their significant effect on the profitability and sustainability of dairy ventures in India. The present study was undertaken to assess the major reproductive problems of dairy animals as well as to develop and validate the extension strategies for managing reproductive problems. A total of 60 respondents from three villages viz Dilawara, Chundipur and Dhakwada of Karnal district were selected through simple random sampling. Data were gathered through structured interview schedule from dairy farmers who had at least one milch animal. Study revealed that repeat breeding, anestrous, retention of fetal membrane and prolapse were the major reproductive problems of dairy animals as perceived by 46.67, 36.67, 28.33 and 26.67 per cent of respondents respectively. Among 14 selected extension strategies, development and demonstration of video on specific reproductive issues with Rank Based Quotient (RBQ) value of 81.19 and preparation of extension literature with RBQ value of 72.38 were found to be the two best strategies for faster dissemination of information on management of reproductive problems. The mean knowledge gain was found to be 10.75 per cent, 13.28 per cent and 15.23 per cent after exposure to different treatments i.e. booklet, video and both (video+booklet) respectively in the study villages. This explains the effectiveness of extension strategies and warrants the extension personnel to contextualise and deploy appropriate combinations of different extension strategies in order to improve the information delivery system.","PeriodicalId":179606,"journal":{"name":"Vol 72, No 2 (2019)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122814217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-04-17DOI: 10.33785/IJDS.2019.V72I02.008
Karanvir Singh, M. Chandra, G. Kaur, D. Narang, D. Gupta, Anmol Arora, N. Sharma
India Abstract: In the present study, a de novo multiplex polymerase chain reaction using genus specific primers for four organisms’ viz., E. coli , Klebsiella pneumoniae , Staphylococcus aureus and Streptococcus agalactiae were designed to identify and differentiate all the four organisms in a single reaction. Further, DNA was extracted from mastitic milk using three different methods and was subjected to multiplex polymerase chain reaction to compare and identify the best method out of these three methods based on the amplification results of multiplex polymerase chain reaction. It was observed from the study that multiplex PCR was able to amplify three organisms ( Streptococcus agalactiae , Klebsiella pneumoniae and E. coli ) successfully whereas was unable to amplify Staphylococcus aureus in the multiplex PCR. Mutiplex PCR when evaluated on the field samples using mastitic milk revealed SDS Triton DNA extraction method to be superior to Power Food Microbial DNA isolation Kit and Milk Bacterial DNA isolation kit method in isolating the bacterial DNA from the mastitic milk as indicated by amplification in multiplex PCR
{"title":"Development of a multiplex PCR for identification of mastitis causing organisms","authors":"Karanvir Singh, M. Chandra, G. Kaur, D. Narang, D. Gupta, Anmol Arora, N. Sharma","doi":"10.33785/IJDS.2019.V72I02.008","DOIUrl":"https://doi.org/10.33785/IJDS.2019.V72I02.008","url":null,"abstract":"India Abstract: In the present study, a de novo multiplex polymerase chain reaction using genus specific primers for four organisms’ viz., E. coli , Klebsiella pneumoniae , Staphylococcus aureus and Streptococcus agalactiae were designed to identify and differentiate all the four organisms in a single reaction. Further, DNA was extracted from mastitic milk using three different methods and was subjected to multiplex polymerase chain reaction to compare and identify the best method out of these three methods based on the amplification results of multiplex polymerase chain reaction. It was observed from the study that multiplex PCR was able to amplify three organisms ( Streptococcus agalactiae , Klebsiella pneumoniae and E. coli ) successfully whereas was unable to amplify Staphylococcus aureus in the multiplex PCR. Mutiplex PCR when evaluated on the field samples using mastitic milk revealed SDS Triton DNA extraction method to be superior to Power Food Microbial DNA isolation Kit and Milk Bacterial DNA isolation kit method in isolating the bacterial DNA from the mastitic milk as indicated by amplification in multiplex PCR","PeriodicalId":179606,"journal":{"name":"Vol 72, No 2 (2019)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115139203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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