Protein microarray technology was developed with the development of DNA microarray technology.Microar-ray surfaces,arraying and detection systems that have initially been developed for DNA microarray can be adapted to protein microarray.The different surfaces for protein attachment have been developed in order to keep proteins in place and in a functional state.Readout systems based on fluorescence,chemiluminescence,radioactivity have been used to detect complex formation within each microspot.The development of recombinant proteins technology im-proved the sensitivity of detection on microarray.Immunoassays on microarray are scalable,reproducible,quantiliable with little sample and are consequently finding wide use in biomedical research.In this paper,some recent develop-ments in preparation of protein microarray and its application in immunoassays,medical diagnostis and proteome re-search are reviewed.
{"title":"Protein microarray technology","authors":"Heng-jie Cao","doi":"10.1002/3527601554","DOIUrl":"https://doi.org/10.1002/3527601554","url":null,"abstract":"Protein microarray technology was developed with the development of DNA microarray technology.Microar-ray surfaces,arraying and detection systems that have initially been developed for DNA microarray can be adapted to protein microarray.The different surfaces for protein attachment have been developed in order to keep proteins in place and in a functional state.Readout systems based on fluorescence,chemiluminescence,radioactivity have been used to detect complex formation within each microspot.The development of recombinant proteins technology im-proved the sensitivity of detection on microarray.Immunoassays on microarray are scalable,reproducible,quantiliable with little sample and are consequently finding wide use in biomedical research.In this paper,some recent develop-ments in preparation of protein microarray and its application in immunoassays,medical diagnostis and proteome re-search are reviewed.","PeriodicalId":18041,"journal":{"name":"Letters in Biotechnology","volume":"46 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2003-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82365391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：