Pub Date : 2021-03-01DOI: 10.2478/macvetrev-2020-0035
M. Mustapha, Y. Audu, K. Ezema, Jafar Umar Abdulkadir, J. Lawal, A. Balami, L. Adamu, Y. M. Bukar-Kolo
Abstract Diarrhea caused by multidrug-resistant Escherichia coli (E. coli) is an important and common problem in companion animals, especially dogs. Moreover, these dogs may serve as a reservoir of pathogenic strains of E. coli that may cause enteric and extra-intestinal infections in humans and other animals. This study was conducted to investigate the antibiotic susceptibility pattern of E. coli isolates from diarrheic dogs in Maiduguri Metropolis, Borno State, Nigeria. In fecal samples of 200 dogs with diarrhea, 147 E. coli strains (73.5%) were isolated and characterized by the standard bacteriological techniques (culture, biochemical tests, and antimicrobial susceptibility testing). Out of the 147 positive isolates, 45, 50, and 52 were from Elkanemi Park, Magaram, and Sabon gari wards respectively. The isolates show 100% resistance to chloramphenicol, cefuroxime, and ceftriaxone, 96.6% to amoxicillin, and 95.9% to gentamicin, while all (100%) were susceptible to ciprofloxacin. All the isolates showed multiple antimicrobial resistance. The result of the current study showed that dogs in Maiduguri are important reservoirs of multidrug-resistant E. coli. Therefore, it is important to adopt and apply guidelines for the correct use of antimicrobials in small animal practice to reduce the emergence of multidrug resistance among E. coli in companion animals.
{"title":"Antimicrobial Susceptibility Profiles of Escherichia Coli Isolates from Diarrheic Dogs in Maiduguri, Borno State, Nigeria","authors":"M. Mustapha, Y. Audu, K. Ezema, Jafar Umar Abdulkadir, J. Lawal, A. Balami, L. Adamu, Y. M. Bukar-Kolo","doi":"10.2478/macvetrev-2020-0035","DOIUrl":"https://doi.org/10.2478/macvetrev-2020-0035","url":null,"abstract":"Abstract Diarrhea caused by multidrug-resistant Escherichia coli (E. coli) is an important and common problem in companion animals, especially dogs. Moreover, these dogs may serve as a reservoir of pathogenic strains of E. coli that may cause enteric and extra-intestinal infections in humans and other animals. This study was conducted to investigate the antibiotic susceptibility pattern of E. coli isolates from diarrheic dogs in Maiduguri Metropolis, Borno State, Nigeria. In fecal samples of 200 dogs with diarrhea, 147 E. coli strains (73.5%) were isolated and characterized by the standard bacteriological techniques (culture, biochemical tests, and antimicrobial susceptibility testing). Out of the 147 positive isolates, 45, 50, and 52 were from Elkanemi Park, Magaram, and Sabon gari wards respectively. The isolates show 100% resistance to chloramphenicol, cefuroxime, and ceftriaxone, 96.6% to amoxicillin, and 95.9% to gentamicin, while all (100%) were susceptible to ciprofloxacin. All the isolates showed multiple antimicrobial resistance. The result of the current study showed that dogs in Maiduguri are important reservoirs of multidrug-resistant E. coli. Therefore, it is important to adopt and apply guidelines for the correct use of antimicrobials in small animal practice to reduce the emergence of multidrug resistance among E. coli in companion animals.","PeriodicalId":18099,"journal":{"name":"Macedonian Veterinary Review","volume":"44 1","pages":"47 - 53"},"PeriodicalIF":0.4,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44690744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-03-01DOI: 10.2478/macvetrev-2021-0012
Siavash Ghaderi, S. Mahdavi, A. Yousefi
Abstract Listeria monocytogenes is one of the most frequent foodborne bacteria that can be transmitted through dairy products. The demand for replacing chemical preservatives with natural compounds has increased recently. The aim of this study was to investigate the effect of Echinophora platyloba DC ethanolic extract on the survival of L. monocytogenes in Lighvan cheese during ripening time. Three concentrations of E. platyloba ethanolic extract (0, 0.5, 1 and 1.5%) were added to raw milk at the beginning of Lighvan cheese manufacture, and the population of L. monocytogenes was counted on days 15, 30, 60 and 90. Furthermore, the pH and salt concentration of Lighvan cheese were evaluated in these periods. The results showed that an increase in the concentration of ethanolic extract, as well as the ripening time of Lighvan cheese, resulted in a significant decrease (p<0.05) in the levels of L. monocytogenes (cfu/g). Moreover, the logarithm of the L. monocytogenes population (log cfu/g) was significantly decreased (p<0.05) as the concentration of the added ethanolic extract was enhanced over the ripening time, but no significant changes in pH and salt concentration were observed in Lighvan cheese (p>0.05). It was found that the optimal concentration of E. platyloba ethanolic extract for the complete inhibition of L. monocytogenes was 1-1.5% following 90 days of the ripening.
{"title":"Study of the Effect of Echinophora platyloba Ethanolic Extract on Survival of Listeria monocytogenes in Lighvan Cheese During Ripening Time","authors":"Siavash Ghaderi, S. Mahdavi, A. Yousefi","doi":"10.2478/macvetrev-2021-0012","DOIUrl":"https://doi.org/10.2478/macvetrev-2021-0012","url":null,"abstract":"Abstract Listeria monocytogenes is one of the most frequent foodborne bacteria that can be transmitted through dairy products. The demand for replacing chemical preservatives with natural compounds has increased recently. The aim of this study was to investigate the effect of Echinophora platyloba DC ethanolic extract on the survival of L. monocytogenes in Lighvan cheese during ripening time. Three concentrations of E. platyloba ethanolic extract (0, 0.5, 1 and 1.5%) were added to raw milk at the beginning of Lighvan cheese manufacture, and the population of L. monocytogenes was counted on days 15, 30, 60 and 90. Furthermore, the pH and salt concentration of Lighvan cheese were evaluated in these periods. The results showed that an increase in the concentration of ethanolic extract, as well as the ripening time of Lighvan cheese, resulted in a significant decrease (p<0.05) in the levels of L. monocytogenes (cfu/g). Moreover, the logarithm of the L. monocytogenes population (log cfu/g) was significantly decreased (p<0.05) as the concentration of the added ethanolic extract was enhanced over the ripening time, but no significant changes in pH and salt concentration were observed in Lighvan cheese (p>0.05). It was found that the optimal concentration of E. platyloba ethanolic extract for the complete inhibition of L. monocytogenes was 1-1.5% following 90 days of the ripening.","PeriodicalId":18099,"journal":{"name":"Macedonian Veterinary Review","volume":"44 1","pages":"79 - 85"},"PeriodicalIF":0.4,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45612111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-03-01DOI: 10.2478/macvetrev-2020-0032
M. A. Ramadhan, Abbas Najee Balasm, S. B. Kadhem, H. F. Al-Saedi
Abstract The treatment of full-thickness skin burn using nanomaterials is promising as a medical application reducing the risk of infection and severe dermal scarring. Therefore, this study aims to evaluate the effectiveness of nanomaterials, particularly 3% silver nanoparticles containing ointment (3% SNO), on the full-thickness skin burn of laboratory mice. A total number of 36 male mice were used, equally divided into three groups: negative control (not burned and not treated); positive control (+ve) (burned and treated with castor oil and white petroleum jelly); and SNO-treated group (burned and treated with 3% SNO). The skin of the animals’ back was shaved. A 2x0.5 cm metal plate was heated on a burner to burn the skin of the animals of positive control and SNO-treated groups. Pseudomonas aeruginosa bacterial suspension was applied to the burnt area. The application of SNO, as well as the mixture of white petroleum jelly and castor oil, was started after 6 hours of inducing burns and continued for 14 days (three times daily) in the respected groups. The SNO-treated group showed accelerated healing within 14 days demonstrated by re-epithelialization of the epidermal layer and proliferation of the fibroblasts in the dermal layer. Less healing evidence was observed in the +ve control group in the same period. In conclusion, to our knowledge, this is the first study that uses a 3% SNO formula and has found that it has a promising impact on the treatment of infected skin burns.
{"title":"Effect of Silver Nanoparticles on Healing of Third-Degree Burns Infected with Pseudomonas Aeruginosa in Laboratory Mice","authors":"M. A. Ramadhan, Abbas Najee Balasm, S. B. Kadhem, H. F. Al-Saedi","doi":"10.2478/macvetrev-2020-0032","DOIUrl":"https://doi.org/10.2478/macvetrev-2020-0032","url":null,"abstract":"Abstract The treatment of full-thickness skin burn using nanomaterials is promising as a medical application reducing the risk of infection and severe dermal scarring. Therefore, this study aims to evaluate the effectiveness of nanomaterials, particularly 3% silver nanoparticles containing ointment (3% SNO), on the full-thickness skin burn of laboratory mice. A total number of 36 male mice were used, equally divided into three groups: negative control (not burned and not treated); positive control (+ve) (burned and treated with castor oil and white petroleum jelly); and SNO-treated group (burned and treated with 3% SNO). The skin of the animals’ back was shaved. A 2x0.5 cm metal plate was heated on a burner to burn the skin of the animals of positive control and SNO-treated groups. Pseudomonas aeruginosa bacterial suspension was applied to the burnt area. The application of SNO, as well as the mixture of white petroleum jelly and castor oil, was started after 6 hours of inducing burns and continued for 14 days (three times daily) in the respected groups. The SNO-treated group showed accelerated healing within 14 days demonstrated by re-epithelialization of the epidermal layer and proliferation of the fibroblasts in the dermal layer. Less healing evidence was observed in the +ve control group in the same period. In conclusion, to our knowledge, this is the first study that uses a 3% SNO formula and has found that it has a promising impact on the treatment of infected skin burns.","PeriodicalId":18099,"journal":{"name":"Macedonian Veterinary Review","volume":"44 1","pages":"17 - 28"},"PeriodicalIF":0.4,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47629120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-03-01DOI: 10.2478/macvetrev-2021-0011
J. Omeragić, D. Alagić, S. Šerić-Haračić, N. Kapo, Darinka Klarić Soldo, E. Šabić, Ć. Crnkić, D. Hadžijunuzović-Alagić, E. Aganović, V. Škapur
Abstract More than 30 dog parasite species have been identified in Bosnia and Herzegovina so far, and half of these are zoonotic. The aim of the study was to investigate the occurrence of parasitic infections in dogs from Bosnian-podrinje canton with a focus on zoonotic parasites. The study included 212 dogs (107 owned and 105 stray). One or more of the twenty parasite species were found in 82.55% of tested samples. Dicrocoelium dendriticum (Class Trematoda) was found in 0.94% of dogs. Dipylidium caninum and Mesocestoides lineatus (Class Cestoda) were found in 7.55% and 0.94% of dogs, respectively. Taenia spp. and Echinococcus spp. (Family Taeniidae) were found in 0.47% and 0.94% of samples, respectively. In the family of Nematoda, the following parasite species were identified with corresponding percent among tested dogs: Toxocara canis (25.94%), Toxascaris leonine (8.96%), Strongyloides stercoralis (0.47%), Ancylostoma caninum (3.77%), Uncinaria stenocephala (52.36%), Trichuris vulpis (22.17%), Eucoleus aerophilus (1.42%), Dirofilaria immitis (1.89%) and Dirofilaria repens (1.42%). In the kingdom of Protozoa, the following parasite species were identified with corresponding percent among tested dogs: Cystoisospora canis (8.49%), C. ohioensis (2.36%), Cryptosporidium spp. (4.72%), Sarcocystis spp. (0.47%), Babesia spp. (5.19%), and Giardia duodenalis (15.57%). Leishmania infantum was not identified in tested samples. The results showed a high level of parasitic infestation in the dog population in the Bosnian-Podrinje canton, with a high occurrence of zoonotic parasites. Significant differences were found between owned and stray dogs for all classes of detected parasites with exception of the class Trematoda, disregarding specific parasite species.
{"title":"Zoonotic Endoparasites in Dogs from the Bosnian-Podrinje Canton, Bosnia and Herzegovina","authors":"J. Omeragić, D. Alagić, S. Šerić-Haračić, N. Kapo, Darinka Klarić Soldo, E. Šabić, Ć. Crnkić, D. Hadžijunuzović-Alagić, E. Aganović, V. Škapur","doi":"10.2478/macvetrev-2021-0011","DOIUrl":"https://doi.org/10.2478/macvetrev-2021-0011","url":null,"abstract":"Abstract More than 30 dog parasite species have been identified in Bosnia and Herzegovina so far, and half of these are zoonotic. The aim of the study was to investigate the occurrence of parasitic infections in dogs from Bosnian-podrinje canton with a focus on zoonotic parasites. The study included 212 dogs (107 owned and 105 stray). One or more of the twenty parasite species were found in 82.55% of tested samples. Dicrocoelium dendriticum (Class Trematoda) was found in 0.94% of dogs. Dipylidium caninum and Mesocestoides lineatus (Class Cestoda) were found in 7.55% and 0.94% of dogs, respectively. Taenia spp. and Echinococcus spp. (Family Taeniidae) were found in 0.47% and 0.94% of samples, respectively. In the family of Nematoda, the following parasite species were identified with corresponding percent among tested dogs: Toxocara canis (25.94%), Toxascaris leonine (8.96%), Strongyloides stercoralis (0.47%), Ancylostoma caninum (3.77%), Uncinaria stenocephala (52.36%), Trichuris vulpis (22.17%), Eucoleus aerophilus (1.42%), Dirofilaria immitis (1.89%) and Dirofilaria repens (1.42%). In the kingdom of Protozoa, the following parasite species were identified with corresponding percent among tested dogs: Cystoisospora canis (8.49%), C. ohioensis (2.36%), Cryptosporidium spp. (4.72%), Sarcocystis spp. (0.47%), Babesia spp. (5.19%), and Giardia duodenalis (15.57%). Leishmania infantum was not identified in tested samples. The results showed a high level of parasitic infestation in the dog population in the Bosnian-Podrinje canton, with a high occurrence of zoonotic parasites. Significant differences were found between owned and stray dogs for all classes of detected parasites with exception of the class Trematoda, disregarding specific parasite species.","PeriodicalId":18099,"journal":{"name":"Macedonian Veterinary Review","volume":"44 1","pages":"63 - 70"},"PeriodicalIF":0.4,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44615482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-03-01DOI: 10.2478/macvetrev-2020-0036
S. Dabravolski, Y. Kavalionak
Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an RNA virus, responsible for the current pandemic outbreak. In total, 200 genomes of the SARS-CoV-2 strains from four host organisms have been analyzed. To investigate the presence of the new mutations in the RNA-directed RNA Polymerase (RdRp) of SARS-CoV-2, we analyzed sequences isolated from different hosts, with particular emphasis on human isolates. We performed a search for the new mutations of the RdRp proteins and study how those newly identified mutations could influence RdRp protein stability. Our results revealed 25 mutations in Rhinolophus sinicus, 1 in Mustela lutreola, 6 in Homo sapiens, and none in Mus musculus RdRp proteins of the SARS-CoV-2 isolates. We found that P323L is the most common stabilising radical mutation in human isolates. Also, we described several unique mutations, specific for studied hosts. Therefore, our data suggest that new and emerging variants of the SARS-CoV-2 RdRp have to be considered for the development of effective therapeutic agents and treatments.
{"title":"The Worldwide Search for the New Mutations in the RNA-Directed RNA Polymerase Domain of SARS-CoV-2","authors":"S. Dabravolski, Y. Kavalionak","doi":"10.2478/macvetrev-2020-0036","DOIUrl":"https://doi.org/10.2478/macvetrev-2020-0036","url":null,"abstract":"Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an RNA virus, responsible for the current pandemic outbreak. In total, 200 genomes of the SARS-CoV-2 strains from four host organisms have been analyzed. To investigate the presence of the new mutations in the RNA-directed RNA Polymerase (RdRp) of SARS-CoV-2, we analyzed sequences isolated from different hosts, with particular emphasis on human isolates. We performed a search for the new mutations of the RdRp proteins and study how those newly identified mutations could influence RdRp protein stability. Our results revealed 25 mutations in Rhinolophus sinicus, 1 in Mustela lutreola, 6 in Homo sapiens, and none in Mus musculus RdRp proteins of the SARS-CoV-2 isolates. We found that P323L is the most common stabilising radical mutation in human isolates. Also, we described several unique mutations, specific for studied hosts. Therefore, our data suggest that new and emerging variants of the SARS-CoV-2 RdRp have to be considered for the development of effective therapeutic agents and treatments.","PeriodicalId":18099,"journal":{"name":"Macedonian Veterinary Review","volume":"44 1","pages":"87 - 94"},"PeriodicalIF":0.4,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45891596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-03-01DOI: 10.2478/macvetrev-2021-0014
Marija Ratkova Manovska, M. Prodanov, L. Angelovski, D. Jankuloski, P. Sekulovski
Abstract Staphylococcus aureus is an important foodborne pathogen due to toxin-related virulence, invasiveness and antibiotic resistance. The ability of S. aureus strains to produce one or more staphylococcal enterotoxins (SEs) in food has been associated with the occurrence of staphylococcal food poisoning (SFP), which is the most common foodborne intoxication worldwide. The study aimed to determine the count of S. aureus strains in samples of raw cow’s milk and various cheeses produced in R. North Macedonia and to detect their ability to produce enterotoxins by passive agglutination SET RPLA (OXOID, UK) and by enzyme-linked fluorescence assay (ELFA) VIDAS SET 2 (Biomerieux, France). A total of 130 S. aureus strains were analyzed. The ability to produce SEs was determined in 17 (13.1%) strains using the SET RPLA detection kit and in 20 (15.4%) strains using the VIDAS SET 2. The study detected enterotoxigenic strains in cheese samples, despite the low count of S. aureus which was below the detection limit according to the Book of rules for microbiological criteria (Off. G. of R.M no 100/2013). Based on these and similar findings, S. aureus must be considered as a possible cause of intoxication, despite the undetected and underreported cases of SFP in the scientific literature.
摘要金黄色葡萄球菌是一种重要的食源性病原体，具有与毒素相关的毒力、侵袭性和抗生素耐药性。金黄色葡萄球菌菌株在食物中产生一种或多种葡萄球菌肠毒素（SE）的能力与葡萄球菌食物中毒（SFP）的发生有关，SFP是世界上最常见的食源性中毒。该研究旨在测定北马其顿生产的生牛奶和各种奶酪样本中金黄色葡萄球菌菌株的数量，并通过被动凝集SET RPLA（OXOID，英国）和酶联荧光分析（ELFA）VIDAS SET 2（Biomerieux，法国）检测其产生肠毒素的能力。共分析了130株金黄色葡萄球菌。在使用SET RPLA检测试剂盒的17个（13.1%）菌株和使用VIDAS SET 2的20个（15.4%）菌株中测定了产生SE的能力。该研究在奶酪样品中检测到了肠毒素菌株，尽管金黄色葡萄球菌计数较低，低于微生物标准规则书的检测限（R.M第100/2013号Off.G.）。基于这些和类似的发现，尽管科学文献中存在未被发现和少报的SFP病例，但金黄色葡萄球菌必须被视为中毒的可能原因。
{"title":"Prevalence of the Enterotoxigenic Staphylococcus Aureus Strains Isolated from Raw Milk and Cheese Produced in North Macedonia","authors":"Marija Ratkova Manovska, M. Prodanov, L. Angelovski, D. Jankuloski, P. Sekulovski","doi":"10.2478/macvetrev-2021-0014","DOIUrl":"https://doi.org/10.2478/macvetrev-2021-0014","url":null,"abstract":"Abstract Staphylococcus aureus is an important foodborne pathogen due to toxin-related virulence, invasiveness and antibiotic resistance. The ability of S. aureus strains to produce one or more staphylococcal enterotoxins (SEs) in food has been associated with the occurrence of staphylococcal food poisoning (SFP), which is the most common foodborne intoxication worldwide. The study aimed to determine the count of S. aureus strains in samples of raw cow’s milk and various cheeses produced in R. North Macedonia and to detect their ability to produce enterotoxins by passive agglutination SET RPLA (OXOID, UK) and by enzyme-linked fluorescence assay (ELFA) VIDAS SET 2 (Biomerieux, France). A total of 130 S. aureus strains were analyzed. The ability to produce SEs was determined in 17 (13.1%) strains using the SET RPLA detection kit and in 20 (15.4%) strains using the VIDAS SET 2. The study detected enterotoxigenic strains in cheese samples, despite the low count of S. aureus which was below the detection limit according to the Book of rules for microbiological criteria (Off. G. of R.M no 100/2013). Based on these and similar findings, S. aureus must be considered as a possible cause of intoxication, despite the undetected and underreported cases of SFP in the scientific literature.","PeriodicalId":18099,"journal":{"name":"Macedonian Veterinary Review","volume":"44 1","pages":"71 - 77"},"PeriodicalIF":0.4,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48448036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-10-01DOI: 10.2478/macvetrev-2020-0020
P. Vidricková, M. Boldižár
Abstract Providing a secure airway management during general anesthesia could be problematic in some medical cases, especially when there is a risk of regurgitation and aspiration of the gastric content due to increased intragastric pressure. The current study aimed to test the applicability of two types of LMA in several animal species and to compare its effectiveness to the endotracheal intubation method in securing sealed airway respiration as an alternative to using endotracheal tubes. The study was conducted in dogs (n=33), cats (n=9), swine (n=9), rabbits (n=5), sheep (n=7) and roe deer (n=1). One or both types of laryngeal masks were used for each animal species: LMA Classic™-cLMA and LMA ProSeal™-PLMA. The assessment of each laryngeal mask was performed by determining the insertion technique, the possibilities of first-attempt insertion and malposition, the compliance with various animal species, ventilation time, cuff pressure, and sealing capacity. The highest LMA size compatility in dogs (23,87±14,30 kg) was size-3 in six and size-4 in forteen subjects; In swine (43,22±12,32 kg), size-4; In rabbits (3,84±0,36 kg) size-1; and in sheep (48,29±4,65 kg) size-3 and size-4. Ventilation time was highest in swine and roe deer (121,11±42,85 min and 300,00 min, respectively) and lowest in cat (28,33±16,96 min). First-attempt LMA insertion success was lowest in rabbits (60%), and highest in sheep and roe deer (100%). Malposition was with highest rate in rabbits (40%) and lowest in cat, sheep and roe deer (0%). Gastric reflux was most frequently observed in sheep (71,4%) and roe deer (100%). The usage of LMA in the veterinary anesthetic practice significantly improves airway management in animals during general anesthesia. The inflated LMA cuff does not prevent its disposition. Therefore, both the drain and respiratory tubes must be fixed. The usage of LMA in rabbits was associated with higher incidence of malposition and other complications. Our findings suggest that LMA designed for humans can be used for airway management in veterinary medicine.
{"title":"Usage of Laryngeal Mask Airway Devices in Veterinary Medicine","authors":"P. Vidricková, M. Boldižár","doi":"10.2478/macvetrev-2020-0020","DOIUrl":"https://doi.org/10.2478/macvetrev-2020-0020","url":null,"abstract":"Abstract Providing a secure airway management during general anesthesia could be problematic in some medical cases, especially when there is a risk of regurgitation and aspiration of the gastric content due to increased intragastric pressure. The current study aimed to test the applicability of two types of LMA in several animal species and to compare its effectiveness to the endotracheal intubation method in securing sealed airway respiration as an alternative to using endotracheal tubes. The study was conducted in dogs (n=33), cats (n=9), swine (n=9), rabbits (n=5), sheep (n=7) and roe deer (n=1). One or both types of laryngeal masks were used for each animal species: LMA Classic™-cLMA and LMA ProSeal™-PLMA. The assessment of each laryngeal mask was performed by determining the insertion technique, the possibilities of first-attempt insertion and malposition, the compliance with various animal species, ventilation time, cuff pressure, and sealing capacity. The highest LMA size compatility in dogs (23,87±14,30 kg) was size-3 in six and size-4 in forteen subjects; In swine (43,22±12,32 kg), size-4; In rabbits (3,84±0,36 kg) size-1; and in sheep (48,29±4,65 kg) size-3 and size-4. Ventilation time was highest in swine and roe deer (121,11±42,85 min and 300,00 min, respectively) and lowest in cat (28,33±16,96 min). First-attempt LMA insertion success was lowest in rabbits (60%), and highest in sheep and roe deer (100%). Malposition was with highest rate in rabbits (40%) and lowest in cat, sheep and roe deer (0%). Gastric reflux was most frequently observed in sheep (71,4%) and roe deer (100%). The usage of LMA in the veterinary anesthetic practice significantly improves airway management in animals during general anesthesia. The inflated LMA cuff does not prevent its disposition. Therefore, both the drain and respiratory tubes must be fixed. The usage of LMA in rabbits was associated with higher incidence of malposition and other complications. Our findings suggest that LMA designed for humans can be used for airway management in veterinary medicine.","PeriodicalId":18099,"journal":{"name":"Macedonian Veterinary Review","volume":"43 1","pages":"131 - 139"},"PeriodicalIF":0.4,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45991745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-10-01DOI: 10.2478/macvetrev-2020-0022
Rosa Kebbi, O. Besseboua, Melaaz Belhadj-Kebbi, L. Hassissen, A. Ayad
Abstract The present study was aimed to evaluate hematological and oxidative stress parameters in domestic dogs infested naturally (n=10) by Rhipicephalus sp. to compare with non-infested dogs (n=10). All blood samples were collected from brachial vein into tubes EDTA for the hematological analysis such as red blood cells (RBCs), white blood cells (WBCs), hemoglobin (HGB) and platelets (PLT). Serum was rapidly separated after centrifugation and stored at -20 °C until it was used for malondialdehyde (MDA) and 2,2’-Azinobis-(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS) inhibition measurements. HGB in non-infested dogs was significantly higher than in infested dogs (P<0.05). There was no significant difference in RBCs, WBCs and PLT between both groups (P>0.05). The mean of MDA concentration was high in infested dogs (0.92±0.62 nmol/ml) compared to non-infested dogs (0.75±0.25 nmol/ml). On the other hand, the percentage of ABTS inhibition was similar in both groups (P=0.71). High tick number seems significantly affected WBCs (P<0.0001) and HGB (P<0.001) in infested dogs. Concerning oxidative status, there was no significant differences (P>0.05) between low and high infested dogs, neither in the amount of MDA nor in the ABTS inhibition. In conclusion, infested dogs induced RBCs alterations, which coincided with the oxidative damage, as evidenced by MDA serum levels. Also, there was a relationship between the tick number in infested dogs and the hematological parameters.
{"title":"Hematological and Oxidative Status Parameters in Domestic Dogs Naturally Infested by Rhipicephalus Sp.","authors":"Rosa Kebbi, O. Besseboua, Melaaz Belhadj-Kebbi, L. Hassissen, A. Ayad","doi":"10.2478/macvetrev-2020-0022","DOIUrl":"https://doi.org/10.2478/macvetrev-2020-0022","url":null,"abstract":"Abstract The present study was aimed to evaluate hematological and oxidative stress parameters in domestic dogs infested naturally (n=10) by Rhipicephalus sp. to compare with non-infested dogs (n=10). All blood samples were collected from brachial vein into tubes EDTA for the hematological analysis such as red blood cells (RBCs), white blood cells (WBCs), hemoglobin (HGB) and platelets (PLT). Serum was rapidly separated after centrifugation and stored at -20 °C until it was used for malondialdehyde (MDA) and 2,2’-Azinobis-(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS) inhibition measurements. HGB in non-infested dogs was significantly higher than in infested dogs (P<0.05). There was no significant difference in RBCs, WBCs and PLT between both groups (P>0.05). The mean of MDA concentration was high in infested dogs (0.92±0.62 nmol/ml) compared to non-infested dogs (0.75±0.25 nmol/ml). On the other hand, the percentage of ABTS inhibition was similar in both groups (P=0.71). High tick number seems significantly affected WBCs (P<0.0001) and HGB (P<0.001) in infested dogs. Concerning oxidative status, there was no significant differences (P>0.05) between low and high infested dogs, neither in the amount of MDA nor in the ABTS inhibition. In conclusion, infested dogs induced RBCs alterations, which coincided with the oxidative damage, as evidenced by MDA serum levels. Also, there was a relationship between the tick number in infested dogs and the hematological parameters.","PeriodicalId":18099,"journal":{"name":"Macedonian Veterinary Review","volume":"43 1","pages":"103 - 110"},"PeriodicalIF":0.4,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45292632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-10-01DOI: 10.2478/macvetrev-2020-0026
T. Ivanova, G. Krumova-Valcheva, G. Mateva, H. Daskalov
Abstract This paper presents an outbreak provoked by methicillin-resistant strains of coagulase-positive S. aureus (CPS), produced staphylococcal enterotoxins (se) in pig meatballs and potato salad consumed from 70 people in the village Mamarchevo, Bulgaria. Eighteen women aged 50 to 70 years, and two children aged 4 and 5 years have demonstrated a severe malaise with vomiting and indigestion. Two food samples and isolates of CPS were received in the laboratory of Bulgarian Food Safety Agency. Both samples were found to have a high level of CPS. The level of S. aureus contamination in the potato salad was 8.3 logs CFU/g and 7.7 logs CFU/g in roasted meatballs, which was a significant reason to doubt the production of the toxin. The samples were analyzed according to the European Screening Method v5 using mini VIDAS SET2. The results showed a presence of staphylococcal enterotoxin (TV 2.67 for meatballs and TV 3.27 for potato salad), which was the reason for the ensuing food intoxication. EURL CPS applying quantitative indirect sandwich-type ELISA confirmed the presence of sea, sec and sed in the potato salad and sea and sed in the roasted meatballs. Two CPS isolates were confirmed as S. aureus by a species-specific 23S rRNA targeted PCR test. Real-time PCR method detected sea, sed, seg, sei, sej, and ser genes in S. aureus strains, found in both matrixes. Multiplex PCR method proved the existence of the mecA gene in both S. aureus strains. Resistance to cefoxitin (>16 mg/L), penicillin (>2 mg/L), kanamycin (64 mg/L) and sulfamethoxazole (>512 mg/L) was found.
{"title":"Characteristics of Staphylococcus aureus Isolated from a Case of Foodborne Outbreak in Bulgaria","authors":"T. Ivanova, G. Krumova-Valcheva, G. Mateva, H. Daskalov","doi":"10.2478/macvetrev-2020-0026","DOIUrl":"https://doi.org/10.2478/macvetrev-2020-0026","url":null,"abstract":"Abstract This paper presents an outbreak provoked by methicillin-resistant strains of coagulase-positive S. aureus (CPS), produced staphylococcal enterotoxins (se) in pig meatballs and potato salad consumed from 70 people in the village Mamarchevo, Bulgaria. Eighteen women aged 50 to 70 years, and two children aged 4 and 5 years have demonstrated a severe malaise with vomiting and indigestion. Two food samples and isolates of CPS were received in the laboratory of Bulgarian Food Safety Agency. Both samples were found to have a high level of CPS. The level of S. aureus contamination in the potato salad was 8.3 logs CFU/g and 7.7 logs CFU/g in roasted meatballs, which was a significant reason to doubt the production of the toxin. The samples were analyzed according to the European Screening Method v5 using mini VIDAS SET2. The results showed a presence of staphylococcal enterotoxin (TV 2.67 for meatballs and TV 3.27 for potato salad), which was the reason for the ensuing food intoxication. EURL CPS applying quantitative indirect sandwich-type ELISA confirmed the presence of sea, sec and sed in the potato salad and sea and sed in the roasted meatballs. Two CPS isolates were confirmed as S. aureus by a species-specific 23S rRNA targeted PCR test. Real-time PCR method detected sea, sed, seg, sei, sej, and ser genes in S. aureus strains, found in both matrixes. Multiplex PCR method proved the existence of the mecA gene in both S. aureus strains. Resistance to cefoxitin (>16 mg/L), penicillin (>2 mg/L), kanamycin (64 mg/L) and sulfamethoxazole (>512 mg/L) was found.","PeriodicalId":18099,"journal":{"name":"Macedonian Veterinary Review","volume":"43 1","pages":"151 - 159"},"PeriodicalIF":0.4,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41580867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-10-01DOI: 10.2478/macvetrev-2020-0023
Lola Romanos, R. Maillard
Abstract Tick-borne diseases are highly prevalent in domestic and wild ruminants and they may be distributed in wide geographical ranges by animal transportation. The aim of the current study was to investigate the presence of European strains of Babesia spp. and/or Anaplasma spp. in oversea imported reindeer specimens. Imported specimens (n=7) were hospitalized with visible tick infestation (Ixodes ricinus) and signs of cachexia, anemia, and hemoglobinuria. Using blood smears, PCR, and BLAST comparisons, it was confirmed that the animals were infected with a French strain of Anaplasma phagocytophilum and Babesia divergens which is considered to be absent in the USA. We conclude that oversea importation of reindeers must be followed with a routine check for geographically-specific strains of pathogens from the place of origin. This monitoring process must be dynamic and according to recent reports of tick-borne pathogens.
{"title":"Severe Anaplasma phagocytophilum and Babesia divergens Concomitant Infection in Imported Captive Reindeer (Rangifer tarandus)","authors":"Lola Romanos, R. Maillard","doi":"10.2478/macvetrev-2020-0023","DOIUrl":"https://doi.org/10.2478/macvetrev-2020-0023","url":null,"abstract":"Abstract Tick-borne diseases are highly prevalent in domestic and wild ruminants and they may be distributed in wide geographical ranges by animal transportation. The aim of the current study was to investigate the presence of European strains of Babesia spp. and/or Anaplasma spp. in oversea imported reindeer specimens. Imported specimens (n=7) were hospitalized with visible tick infestation (Ixodes ricinus) and signs of cachexia, anemia, and hemoglobinuria. Using blood smears, PCR, and BLAST comparisons, it was confirmed that the animals were infected with a French strain of Anaplasma phagocytophilum and Babesia divergens which is considered to be absent in the USA. We conclude that oversea importation of reindeers must be followed with a routine check for geographically-specific strains of pathogens from the place of origin. This monitoring process must be dynamic and according to recent reports of tick-borne pathogens.","PeriodicalId":18099,"journal":{"name":"Macedonian Veterinary Review","volume":"43 1","pages":"185 - 191"},"PeriodicalIF":0.4,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48302864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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