首页 > 最新文献

Microbes and Environments最新文献

英文 中文
Weekly Observations of Estuarine Microbial Assemblages during Summer in the Inner Part of Ariake Bay, Japan; Microbial Water-sediment Coupling in Turbid Shallow Waters 日本有明湾中部夏季河口微生物群落的周观测浑浊浅水微生物水-沉积物耦合研究
IF 2.2 4区 环境科学与生态学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2022-06-08 DOI: 10.1264/jsme2.ME22015
R. Orita, K. Yoshida, Hiroto Terazono, Y. Nagano, M. Goto, K. Kimura, Genta Kobayashi
Estuarine microbial assemblages are altered by a number of environmental factors, and knowledge of these changes is essential for understanding the functions of microbes in estuarine ecosystems. The aims of the present study were to examine the relationship between microbial assemblages in the water column and sediment surface, and to identify the environmental factors that influence the short-term dynamics of microbial assemblages in these two zones in summer in the inner part of Ariake Bay. The microbial assemblage of each sample consisted of a mean of 71.1% operational taxonomic units (OTUs), which commonly occurred in the water column and sediment surface, although their relative composition markedly differed between the two zones. In the water column, spatiotemporal changes in microbial assemblages correlated with several environmental factors, such as the nitrogen content in suspended particles, turbidity, and salinity. On the other hand, temporal changes in the sediment’s microbial assemblages were governed by a single environmental factor, namely, the oxygen reduction potential. These results suggest that the composition of microbial assemblages in the water column and sediment surface differed even in highly turbid brackish waters with high sediment resuspension, and the environmental factors contributing to the change in the assemblage composition also differed between the water column and sediment.
河口微生物群落受到许多环境因素的改变,了解这些变化对于理解微生物在河口生态系统中的功能至关重要。本研究的目的是研究Ariake湾内部水柱和沉积物表面微生物组合之间的关系,并确定影响夏季这两个区域微生物组合短期动态的环境因素。每个样本的微生物组合平均由71.1%的操作分类单元(OTU)组成,这些单元通常出现在水柱和沉积物表面,尽管它们的相对组成在两个区域之间存在显著差异。在水柱中,微生物组合的时空变化与几个环境因素有关,如悬浮颗粒中的氮含量、浊度和盐度。另一方面,沉积物微生物组合的时间变化由一个单一的环境因素决定,即氧还原潜力。这些结果表明,即使在高沉积物再悬浮度的高浊度微咸水中,水柱和沉积物表面的微生物组合组成也存在差异,导致组合组成变化的环境因素在水柱和沉积物之间也存在差异。
{"title":"Weekly Observations of Estuarine Microbial Assemblages during Summer in the Inner Part of Ariake Bay, Japan; Microbial Water-sediment Coupling in Turbid Shallow Waters","authors":"R. Orita, K. Yoshida, Hiroto Terazono, Y. Nagano, M. Goto, K. Kimura, Genta Kobayashi","doi":"10.1264/jsme2.ME22015","DOIUrl":"https://doi.org/10.1264/jsme2.ME22015","url":null,"abstract":"Estuarine microbial assemblages are altered by a number of environmental factors, and knowledge of these changes is essential for understanding the functions of microbes in estuarine ecosystems. The aims of the present study were to examine the relationship between microbial assemblages in the water column and sediment surface, and to identify the environmental factors that influence the short-term dynamics of microbial assemblages in these two zones in summer in the inner part of Ariake Bay. The microbial assemblage of each sample consisted of a mean of 71.1% operational taxonomic units (OTUs), which commonly occurred in the water column and sediment surface, although their relative composition markedly differed between the two zones. In the water column, spatiotemporal changes in microbial assemblages correlated with several environmental factors, such as the nitrogen content in suspended particles, turbidity, and salinity. On the other hand, temporal changes in the sediment’s microbial assemblages were governed by a single environmental factor, namely, the oxygen reduction potential. These results suggest that the composition of microbial assemblages in the water column and sediment surface differed even in highly turbid brackish waters with high sediment resuspension, and the environmental factors contributing to the change in the assemblage composition also differed between the water column and sediment.","PeriodicalId":18482,"journal":{"name":"Microbes and Environments","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2022-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44241460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Diversity of Candidatus Patescibacteria in Activated Sludge Revealed by a Size-Fractionation Approach 用粒度分级法揭示活性污泥中念珠菌的多样性
IF 2.2 4区 环境科学与生态学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2022-06-08 DOI: 10.1264/jsme2.me22027
Shuka Kagemasa, Kyohei Kuroda, R. Nakai, Y. Li, K. Kubota
Uncultivated members of Candidatus Patescibacteria are commonly found in activated sludge treating sewage and are widely distributed in wastewater treatment plants in different regions and countries. However, the phylogenetic diversity of Ca. Patescibacteria is difficult to examine because of their low relative abundance in the environment. Since Ca. Patescibacteria members have small cell sizes, we herein collected small microorganisms from activated sludge using a filtration-based size-fractionation approach (i.e., 0.45–0.22 μm and 0.22–0.1 μm fractions). Fractionated samples were characterized using 16S rRNA gene amplicon and shotgun metagenomic sequence analyses. The amplicon analysis revealed that the relative abundance of Ca. Patescibacteria increased to 73.5% and 52.5% in the 0.45–0.22 μm and 0.22–0.1 μm fraction samples, respectively, from 5.8% in the unfractionated sample. The members recovered from the two size-fractionated samples included Ca. Saccharimonadia, Ca. Gracilibacteria, Ca. Paceibacteria, Ca. Microgenomatia, class-level uncultured lineage ABY1, Ca. Berkelbacteria, WS6 (Ca. Dojkabacteria), and WWE3, with Ca. Saccharimonadia being predominant in both fraction samples. The number of operational taxonomic units belonging to Ca. Patescibacteria was approximately 6-fold higher in the size-fractionated samples than in the unfractionated sample. The shotgun metagenomic analysis of the 0.45–0.22 μm fractioned sample enabled the reconstruction of 24 high-quality patescibacterial bins. The bins obtained were classified into diverse clades at the family and genus levels, some of which were rarely detected in previous activated sludge studies. Collectively, the present results suggest that the overall diversity of Ca. Patescibacteria inhabiting activated sludge is higher than previously expected.
未经培养的patescibacterium成员常见于处理污水的活性污泥中,广泛分布于不同地区和国家的污水处理厂。然而,由于Ca. Patescibacteria在环境中的相对丰度较低,因此很难对其系统发育多样性进行研究。由于Ca. Patescibacteria成员的细胞尺寸较小,因此我们在此使用基于过滤的粒径分级方法(即0.45-0.22 μm和0.22-0.1 μm馏分)从活性污泥中收集小型微生物。采用16S rRNA基因扩增子和散弹枪宏基因组序列分析对分离样品进行表征。扩增子分析结果表明,Ca. Patescibacteria的相对丰度在0.45 ~ 0.22 μm和0.22 ~ 0.1 μm样品中分别由未分离样品的5.8%提高到73.5%和52.5%。从两个不同大小的样品中回收的成员包括Ca. Saccharimonadia, Ca. Gracilibacteria, Ca. Paceibacteria, Ca. Microgenomatia,类水平未培养谱系ABY1, Ca. Berkelbacteria, WS6 (Ca. dojkabobacteria)和WWE3,其中Ca. Saccharimonadia在两个样品中都占优势。在大小分级的样品中,属于帕氏Ca. Patescibacteria的操作分类单位的数量比未分级的样品高约6倍。对0.45-0.22 μm分离样品进行散弹枪宏基因组分析,重建了24个高质量的大肠杆菌箱。获得的垃圾箱在科和属水平上被分类为不同的分支,其中一些在以前的活性污泥研究中很少被发现。总的来说,目前的结果表明,寄生在活性污泥中的帕氏钙杆菌的总体多样性比先前预期的要高。
{"title":"Diversity of Candidatus Patescibacteria in Activated Sludge Revealed by a Size-Fractionation Approach","authors":"Shuka Kagemasa, Kyohei Kuroda, R. Nakai, Y. Li, K. Kubota","doi":"10.1264/jsme2.me22027","DOIUrl":"https://doi.org/10.1264/jsme2.me22027","url":null,"abstract":"Uncultivated members of Candidatus Patescibacteria are commonly found in activated sludge treating sewage and are widely distributed in wastewater treatment plants in different regions and countries. However, the phylogenetic diversity of Ca. Patescibacteria is difficult to examine because of their low relative abundance in the environment. Since Ca. Patescibacteria members have small cell sizes, we herein collected small microorganisms from activated sludge using a filtration-based size-fractionation approach (i.e., 0.45–0.22 μm and 0.22–0.1 μm fractions). Fractionated samples were characterized using 16S rRNA gene amplicon and shotgun metagenomic sequence analyses. The amplicon analysis revealed that the relative abundance of Ca. Patescibacteria increased to 73.5% and 52.5% in the 0.45–0.22 μm and 0.22–0.1 μm fraction samples, respectively, from 5.8% in the unfractionated sample. The members recovered from the two size-fractionated samples included Ca. Saccharimonadia, Ca. Gracilibacteria, Ca. Paceibacteria, Ca. Microgenomatia, class-level uncultured lineage ABY1, Ca. Berkelbacteria, WS6 (Ca. Dojkabacteria), and WWE3, with Ca. Saccharimonadia being predominant in both fraction samples. The number of operational taxonomic units belonging to Ca. Patescibacteria was approximately 6-fold higher in the size-fractionated samples than in the unfractionated sample. The shotgun metagenomic analysis of the 0.45–0.22 μm fractioned sample enabled the reconstruction of 24 high-quality patescibacterial bins. The bins obtained were classified into diverse clades at the family and genus levels, some of which were rarely detected in previous activated sludge studies. Collectively, the present results suggest that the overall diversity of Ca. Patescibacteria inhabiting activated sludge is higher than previously expected.","PeriodicalId":18482,"journal":{"name":"Microbes and Environments","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2022-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44441858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Effects of Different Types of Additional Fertilizers on Root-associated Microbes of Napa Cabbage Grown in an Andosol Field in Japan 不同施肥类型对日本安多醇田Napa甘蓝根系相关微生物的影响
IF 2.2 4区 环境科学与生态学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2022-05-31 DOI: 10.1264/jsme2.ME22013
S. Ikeda, K. Okazaki, Hirohito Tsurumaru, Takanori Suzuki, M. Hirafuji
The effects of different types of additional fertilizations (a compound fertilizer and Chiyoda-kasei) on the root-associated microbes of napa cabbage grown in an Andosol field were investigated by molecular community analyses. Most of the closest known species of the bacterial sequences whose relative abundance significantly differed among fertilizers were sensitive to nitrogen fertilization and/or related to the geochemical cycles of nitrogen. The fungal community on the roots of napa cabbage was dominated by two genera, Bipolaris and Olpidium. The relative abundance of these two genera was affected by the types of fertilizers to some extent and showed a strong negative correlation.
通过分子群落分析,研究了不同类型的附加肥(复合肥和千代田肥)对安多醇田napa甘蓝根系相关微生物的影响。大多数已知的细菌序列中最接近的物种,其相对丰度在肥料之间存在显著差异,对氮肥敏感和/或与氮的地球化学循环有关。甘蓝根上的真菌群落主要由两个属组成,即双孢属(Bipolaris)和十字孢属(Olpidium)。这两个属的相对丰度在一定程度上受到肥料类型的影响,并表现出强烈的负相关。
{"title":"Effects of Different Types of Additional Fertilizers on Root-associated Microbes of Napa Cabbage Grown in an Andosol Field in Japan","authors":"S. Ikeda, K. Okazaki, Hirohito Tsurumaru, Takanori Suzuki, M. Hirafuji","doi":"10.1264/jsme2.ME22013","DOIUrl":"https://doi.org/10.1264/jsme2.ME22013","url":null,"abstract":"The effects of different types of additional fertilizations (a compound fertilizer and Chiyoda-kasei) on the root-associated microbes of napa cabbage grown in an Andosol field were investigated by molecular community analyses. Most of the closest known species of the bacterial sequences whose relative abundance significantly differed among fertilizers were sensitive to nitrogen fertilization and/or related to the geochemical cycles of nitrogen. The fungal community on the roots of napa cabbage was dominated by two genera, Bipolaris and Olpidium. The relative abundance of these two genera was affected by the types of fertilizers to some extent and showed a strong negative correlation.","PeriodicalId":18482,"journal":{"name":"Microbes and Environments","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2022-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48037295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A Minority Population of Non-dye-decolorizing Bacillus subtilis enhances the Azo Dye-decolorizing Activity of Enterococcus faecalis 少数非染料脱色枯草芽孢杆菌提高粪肠球菌偶氮染料脱色活性
IF 2.2 4区 环境科学与生态学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2022-05-31 DOI: 10.1264/jsme2.ME21080
Yuta Yamanashi, Tsukasa Ito
Microbes live in communities in biological wastewater treatment plants and in the intestines. However, limited information is currently available on the mechanisms by which minority bacterial populations assist other bacteria besides syntrophic relationships as well as on the microbial food web. Therefore, the present study investigated the effects of non-dye-decolorizing Bacillus subtilis strain S4ga at population levels ranging between 0.04 and 4% on the activity of dye-decolorizing Enterococcus faecalis strain T6a1 using a dye decolorization assay. The results obtained revealed that the minority population of B. subtilis S4ga enhanced the dye-decolorizing activity of E. faecalis T6a1, resulting in a shorter lag time and longer active time of dye decolorization. These effects were related to redox potential values rather than O2 concentrations. Comparisons of the extracellular metabolites in individual incubations of E. faecalis T6a1 and B. subtilis S4ga and a co-incubation suggested a mutual relationship through the cross-feeding of specific amino acids (tyrosine, methionine, tryptophan, phenylalanine, valine, and leucine from B. subtilis S4ga to E. faecalis T6a1; glutamine, histidine, aspartic acid, and proline from E. faecalis T6a1 to B. subtilis S4ga). An analysis of intracellular primary metabolites indicated that the arginine deiminase (ADI) pathway, an ATP-producing energy-generating process, was more strongly activated in co-incubated E. faecalis T6a1 than in E. faecalis T6a1 incubated alone. These results suggest that a co-incubation with B. subtilis S4ga promoted ATP production by E. faecalis T6a1 cells and enhanced its dye-decolorizing activity.
微生物生活在生物废水处理厂的社区和肠道中。然而,目前关于少数细菌种群辅助其他细菌的机制以及微生物食物网的信息有限。因此,本研究使用染料脱色测定法研究了群体水平在0.04%-4%之间的非染料脱色枯草芽孢杆菌菌株S4ga对染料脱色粪肠球菌菌株T6a1的活性的影响。结果表明,枯草芽孢杆菌S4ga的少数群体增强了粪肠球菌T6a1的染料脱色活性,导致染料脱色的滞后时间更短,活性时间更长。这些效应与氧化还原电位值有关,而不是与O2浓度有关。粪便大肠杆菌T6a1和枯草芽孢杆菌S4ga单独孵育和共同孵育中细胞外代谢产物的比较表明,通过特定氨基酸的交叉喂养,存在相互关系(酪氨酸、甲硫氨酸、色氨酸、苯丙氨酸、缬氨酸和亮氨酸,从枯草芽孢杆菌S4ga到粪肠球菌T6a1;谷氨酰胺、组氨酸、天冬氨酸和脯氨酸,从粪杆菌T6a1到枯草芽孢杆菌S4)。对细胞内初级代谢产物的分析表明,精氨酸脱氨酶(ADI)途径是一种产生ATP的能量产生过程,在共同孵育的粪肠球菌T6a1中比单独孵育的粪便肠球菌T6a1更强烈地激活。这些结果表明,与枯草芽孢杆菌S4ga共孵育促进了粪肠球菌T6a1细胞产生ATP,并增强了其染料脱色活性。
{"title":"A Minority Population of Non-dye-decolorizing Bacillus subtilis enhances the Azo Dye-decolorizing Activity of Enterococcus faecalis","authors":"Yuta Yamanashi, Tsukasa Ito","doi":"10.1264/jsme2.ME21080","DOIUrl":"https://doi.org/10.1264/jsme2.ME21080","url":null,"abstract":"Microbes live in communities in biological wastewater treatment plants and in the intestines. However, limited information is currently available on the mechanisms by which minority bacterial populations assist other bacteria besides syntrophic relationships as well as on the microbial food web. Therefore, the present study investigated the effects of non-dye-decolorizing Bacillus subtilis strain S4ga at population levels ranging between 0.04 and 4% on the activity of dye-decolorizing Enterococcus faecalis strain T6a1 using a dye decolorization assay. The results obtained revealed that the minority population of B. subtilis S4ga enhanced the dye-decolorizing activity of E. faecalis T6a1, resulting in a shorter lag time and longer active time of dye decolorization. These effects were related to redox potential values rather than O2 concentrations. Comparisons of the extracellular metabolites in individual incubations of E. faecalis T6a1 and B. subtilis S4ga and a co-incubation suggested a mutual relationship through the cross-feeding of specific amino acids (tyrosine, methionine, tryptophan, phenylalanine, valine, and leucine from B. subtilis S4ga to E. faecalis T6a1; glutamine, histidine, aspartic acid, and proline from E. faecalis T6a1 to B. subtilis S4ga). An analysis of intracellular primary metabolites indicated that the arginine deiminase (ADI) pathway, an ATP-producing energy-generating process, was more strongly activated in co-incubated E. faecalis T6a1 than in E. faecalis T6a1 incubated alone. These results suggest that a co-incubation with B. subtilis S4ga promoted ATP production by E. faecalis T6a1 cells and enhanced its dye-decolorizing activity.","PeriodicalId":18482,"journal":{"name":"Microbes and Environments","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2022-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44778182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Detection and Identification of Novel Intracellular Bacteria Hosted in Strains CBS 648.67 and CFCC 80795 of Biocontrol Fungi Metarhizium 绿僵菌生防菌CBS 648.67和CFCC 80795胞内新型细菌的检测与鉴定
IF 2.2 4区 环境科学与生态学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2022-05-24 DOI: 10.1264/jsme2.ME21059
Yue Ying, Chenglin Liu, Ran He, Ruizhen Wang, Liangjian Qu
“Endosymbiosis” is a cohesive form of a symbiotic association. Endobacteria exist in many fungi and play important roles in fungal host biology. Metarhizium spp. are important entomopathogenic fungi for insect pest control. In the present study, we performed comprehensive analyses of strains of Metarhizium bibionidarum and M. anisopliae using PCR, phylogenetics, and fluorescent electron microscopy to identify endobacteria within hyphae and conidia. The results of the phylogenetic analysis based on 16S rRNA gene sequences indicated that these endobacteria were the most closely related to Pelomonas puraquae and affiliated with Betaproteobacteria. Ultrastructural observations indicated that endobacteria were coccoid and less than 500 nm in diameter. The basic characteristics of endobacteria in M. bibionidarum and M. anisopliae were elucidated, and biological questions were raised regarding their biological functions in the Metarhizium hosts.
“内共生”是一种共生关系的内聚形式。内细菌存在于许多真菌中,在真菌宿主生物学中起着重要作用。绿僵菌属是防治害虫的重要昆虫病原真菌。在本研究中,我们利用PCR、系统发育和荧光电镜对bibionidarum Metarhizium bibionidum和M. anisopliae菌株进行了综合分析,以鉴定菌丝和分生孢子内的内细菌。基于16S rRNA基因序列的系统发育分析结果表明,这些内菌与puraquae Pelomonas亲缘关系最密切,隶属于Betaproteobacteria。超微结构观察表明,内细菌呈球粒状,直径小于500 nm。阐明了bibionidarum和m.s apilpliae内细菌的基本特征,并对其在绿僵菌宿主中的生物学功能提出了疑问。
{"title":"Detection and Identification of Novel Intracellular Bacteria Hosted in Strains CBS 648.67 and CFCC 80795 of Biocontrol Fungi Metarhizium","authors":"Yue Ying, Chenglin Liu, Ran He, Ruizhen Wang, Liangjian Qu","doi":"10.1264/jsme2.ME21059","DOIUrl":"https://doi.org/10.1264/jsme2.ME21059","url":null,"abstract":"“Endosymbiosis” is a cohesive form of a symbiotic association. Endobacteria exist in many fungi and play important roles in fungal host biology. Metarhizium spp. are important entomopathogenic fungi for insect pest control. In the present study, we performed comprehensive analyses of strains of Metarhizium bibionidarum and M. anisopliae using PCR, phylogenetics, and fluorescent electron microscopy to identify endobacteria within hyphae and conidia. The results of the phylogenetic analysis based on 16S rRNA gene sequences indicated that these endobacteria were the most closely related to Pelomonas puraquae and affiliated with Betaproteobacteria. Ultrastructural observations indicated that endobacteria were coccoid and less than 500 nm in diameter. The basic characteristics of endobacteria in M. bibionidarum and M. anisopliae were elucidated, and biological questions were raised regarding their biological functions in the Metarhizium hosts.","PeriodicalId":18482,"journal":{"name":"Microbes and Environments","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2022-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47037085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Isolation and Characterization of Phosphate Solubilizing Bacteria from Paddy Field Soils in Japan 日本稻田土壤中溶磷细菌的分离与鉴定
IF 2.2 4区 环境科学与生态学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2022-05-21 DOI: 10.1264/jsme2.ME21085
Jean Louise Cocson Damo, María Daniela Artigas Ramírez, Shin-ichiro Agake, M. Pedro, Marilyn B. Brown, H. Sekimoto, T. Yokoyama, S. Sugihara, Shin Okazaki, Naoko Ohkama‐Ohtsu
Phosphorus (P) is abundant in soil and is essential for plant growth and development; however, it is easily rendered insoluble in complexes of different types of phosphates, which may lead to P deficiency. Therefore, increases in the amount of P released from phosphate minerals using microbial inoculants is an important aspect of agriculture. The present study used inorganic phosphate solubilizing bacteria (iPSB) in paddy field soils to develop microbial inoculants. Soils planted with rice were collected from different regions of Japan. Soil P was sequentially fractionated using the Hedley method. iPSB were isolated using selective media supplemented with tricalcium phosphate (Ca-P), aluminum phosphate (Al-P), or iron phosphate (Fe-P). Representative isolates were selected based on the P solubilization index and soil sampling site. Identification was performed using 16S rRNA and rpoB gene sequencing. Effectiveness was screened based on rice cultivar Koshihikari growth supplemented with Ca-P, Al-P, or Fe-P as the sole P source. Despite the relatively homogenous soil pH of paddy field sources, three sets of iPSB were isolated, suggesting the influence of fertilizer management and soil types. Most isolates were categorized as β-Proteobacteria (43%). To the best of our knowledge, this is the first study to describe the genera Pleomorphomonas, Rhodanobacter, and Trinickia as iPSB. Acidovorax sp. JC5, Pseudomonas sp. JC11, Burkholderia sp. JA6 and JA10, Sphingomonas sp. JA11, Mycolicibacterium sp. JF5, and Variovorax sp. JF6 promoted plant growth in rice supplemented with an insoluble P source. The iPSBs obtained may be developed as microbial inoculants for various soil types with different P fixation capacities.
磷在土壤中含量丰富,对植物生长发育至关重要;然而,它很容易不溶于不同类型磷酸盐的络合物中,这可能导致磷缺乏。因此,使用微生物接种剂增加磷酸盐矿物释放的磷量是农业的一个重要方面。本研究利用稻田土壤中的无机溶磷菌(iPSB)开发微生物接种剂。种植水稻的土壤是从日本不同地区采集的。采用Hedley法对土壤磷进行了顺序分级。使用补充有磷酸三钙(Ca-P)、磷酸铝(Al-P)或磷酸铁(Fe-P)的选择性培养基分离iPSB。根据溶解磷指数和土壤采样点选择具有代表性的分离株。使用16S rRNA和rpoB基因测序进行鉴定。以添加Ca-P、Al-P或Fe-P作为唯一磷源的水稻品种Koshihikari生长为基础筛选有效性。尽管稻田来源的土壤pH相对均匀,但分离出三组iPSB,这表明肥料管理和土壤类型的影响。大多数分离株被归类为β-变形杆菌(43%)。据我们所知,这是第一项将Pleomorphomonas属、Rhodanobacter属和Trinickia属描述为iPSB的研究。嗜酸菌JC5、假单胞菌JC11、伯克霍尔德菌JA6和JA10、鞘氨醇单胞菌JA11、分枝杆菌JF5和Variovorax JF6在补充不溶性磷源的水稻中促进植物生长。所获得的iPSB可以作为具有不同磷固定能力的各种土壤类型的微生物接种剂进行开发。
{"title":"Isolation and Characterization of Phosphate Solubilizing Bacteria from Paddy Field Soils in Japan","authors":"Jean Louise Cocson Damo, María Daniela Artigas Ramírez, Shin-ichiro Agake, M. Pedro, Marilyn B. Brown, H. Sekimoto, T. Yokoyama, S. Sugihara, Shin Okazaki, Naoko Ohkama‐Ohtsu","doi":"10.1264/jsme2.ME21085","DOIUrl":"https://doi.org/10.1264/jsme2.ME21085","url":null,"abstract":"Phosphorus (P) is abundant in soil and is essential for plant growth and development; however, it is easily rendered insoluble in complexes of different types of phosphates, which may lead to P deficiency. Therefore, increases in the amount of P released from phosphate minerals using microbial inoculants is an important aspect of agriculture. The present study used inorganic phosphate solubilizing bacteria (iPSB) in paddy field soils to develop microbial inoculants. Soils planted with rice were collected from different regions of Japan. Soil P was sequentially fractionated using the Hedley method. iPSB were isolated using selective media supplemented with tricalcium phosphate (Ca-P), aluminum phosphate (Al-P), or iron phosphate (Fe-P). Representative isolates were selected based on the P solubilization index and soil sampling site. Identification was performed using 16S rRNA and rpoB gene sequencing. Effectiveness was screened based on rice cultivar Koshihikari growth supplemented with Ca-P, Al-P, or Fe-P as the sole P source. Despite the relatively homogenous soil pH of paddy field sources, three sets of iPSB were isolated, suggesting the influence of fertilizer management and soil types. Most isolates were categorized as β-Proteobacteria (43%). To the best of our knowledge, this is the first study to describe the genera Pleomorphomonas, Rhodanobacter, and Trinickia as iPSB. Acidovorax sp. JC5, Pseudomonas sp. JC11, Burkholderia sp. JA6 and JA10, Sphingomonas sp. JA11, Mycolicibacterium sp. JF5, and Variovorax sp. JF6 promoted plant growth in rice supplemented with an insoluble P source. The iPSBs obtained may be developed as microbial inoculants for various soil types with different P fixation capacities.","PeriodicalId":18482,"journal":{"name":"Microbes and Environments","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2022-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47554913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Bacterial Community of Water Yam (Dioscorea alata L.) cv. A-19 水山药的细菌群落。19
IF 2.2 4区 环境科学与生态学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2022-05-03 DOI: 10.1264/jsme2.ME21062
Shunta Kihara, Kosuke Yamamoto, Atsushi Hisatomi, Yuh Shiwa, Chia-Cheng Chu, Kanako Takada, Michel Ouyabe, Babil Pachakkil, H. Kikuno, N. Tanaka, H. Shiwachi
The bacterial community of water yam (Dioscorea alata L.) cv. A-19 is vital because it may promote plant growth without the need for fertilization. However, the influence of fertilization practices on the composition and proportion of the bacterial community of water yam cv. A-19 has not yet been extensively examined. Therefore, we herein investigated the diversity and composition of the bacterial community of water yam cv. A-19 cultivated with and without chemical fertilization using amplicon community profiling based on 16S rRNA gene sequences. No significant difference was detected in the growth of plants cultivated with or without chemical fertilization. Alpha diversity indices were significantly dependent on each compartment, and a decrease was observed in indices from the belowground (rhizosphere and root) to aboveground compartments (stem and leaf). The bacterial composition of each compartment was clustered into three groups: bulk soil, rhizosphere and root, and stem and leaf. Chemical fertilization did not significantly influence the diversity or composition of the water yam cv. A-19 bacterial community. It remained robust in plants cultivated with chemical fertilization. The amplicon community profiling of bacterial communities also revealed the dominance of two bacterial clades, the Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium clade and Burkholderia-Caballeronia-Paraburkholderia clade, with and without chemical fertilization. This is the first study to characterize the bacterial community of water yam cv. A-19 cultivated with and without chemical fertilization.
水山药(Dioscorea alata L.)细菌群落。A-19很重要,因为它不需要施肥就能促进植物生长。然而,施肥方式对山药细菌群落组成和比例的影响。A-19尚未被广泛检查。因此,我们对山药细菌群落的多样性和组成进行了研究。利用基于16S rRNA基因序列的扩增子群落分析,对化学施肥和不施肥的A-19进行了培养。施用和不施用化学肥料的植株生长无显著差异。α多样性指数在各隔室间呈显著依赖性,由地下(根际和根)向地上(茎和叶)呈下降趋势。每个隔室的细菌组成可分为3组:大块土壤、根际和根、茎和叶。化学施肥对山药种群的多样性和组成影响不显著。A-19细菌群落。它在施用化学肥料的植物中仍然很强健。细菌群落扩增子群落分析也显示,在施用和不施用化学肥料的情况下,异源菌-新根菌-副根菌-根瘤菌支系和伯克氏菌-卡氏菌-副根菌支系占优势。本文首次对山药细菌群落进行了研究。有和没有化学施肥栽培的A-19。
{"title":"Bacterial Community of Water Yam (Dioscorea alata L.) cv. A-19","authors":"Shunta Kihara, Kosuke Yamamoto, Atsushi Hisatomi, Yuh Shiwa, Chia-Cheng Chu, Kanako Takada, Michel Ouyabe, Babil Pachakkil, H. Kikuno, N. Tanaka, H. Shiwachi","doi":"10.1264/jsme2.ME21062","DOIUrl":"https://doi.org/10.1264/jsme2.ME21062","url":null,"abstract":"The bacterial community of water yam (Dioscorea alata L.) cv. A-19 is vital because it may promote plant growth without the need for fertilization. However, the influence of fertilization practices on the composition and proportion of the bacterial community of water yam cv. A-19 has not yet been extensively examined. Therefore, we herein investigated the diversity and composition of the bacterial community of water yam cv. A-19 cultivated with and without chemical fertilization using amplicon community profiling based on 16S rRNA gene sequences. No significant difference was detected in the growth of plants cultivated with or without chemical fertilization. Alpha diversity indices were significantly dependent on each compartment, and a decrease was observed in indices from the belowground (rhizosphere and root) to aboveground compartments (stem and leaf). The bacterial composition of each compartment was clustered into three groups: bulk soil, rhizosphere and root, and stem and leaf. Chemical fertilization did not significantly influence the diversity or composition of the water yam cv. A-19 bacterial community. It remained robust in plants cultivated with chemical fertilization. The amplicon community profiling of bacterial communities also revealed the dominance of two bacterial clades, the Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium clade and Burkholderia-Caballeronia-Paraburkholderia clade, with and without chemical fertilization. This is the first study to characterize the bacterial community of water yam cv. A-19 cultivated with and without chemical fertilization.","PeriodicalId":18482,"journal":{"name":"Microbes and Environments","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2022-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44206780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
NH2OH Disproportionation Mediated by Anaerobic Ammonium-oxidizing (Anammox) Bacteria 厌氧氨氧化菌介导的NH2OH歧化反应
IF 2.2 4区 环境科学与生态学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2022-04-12 DOI: 10.1264/jsme2.ME21092
M. Oshiki, Lin Gao, Lei Zhang, S. Okabe
Anammox bacteria produce N2 gas by oxidizing NH4+ with NO2–, and hydroxylamine (NH2OH) is a potential intermediate of the anammox process. N2 gas production occurs when anammox bacteria are incubated with NH2OH only, indicating their capacity for NH2OH disproportionation with NH2OH serving as both the electron donor and acceptor. Limited information is currently available on NH2OH disproportionation by anammox bacteria; therefore, the stoichiometry of anammox bacterial NH2OH disproportionation was examined in the present study using 15N-tracing techniques. The anammox bacteria, Brocadia sinica, Jettenia caeni, and Scalindua sp. were incubated with the addition of 15NH2OH, and the production of 15N-labeled nitrogenous compounds was assessed. The anammox bacteria tested performed NH2OH disproportionation and produced 15-15N2 gas and NH4+ as reaction products. The addition of acetylene, an inhibitor of the anammox process, reduced the activity of NH2OH disproportionation, but not completely. The growth of B. sinica by NH2OH disproportionation (–240.3 kJ mol NH2OH–1 under standard conditions) was also tested in 3 up-flow column anammox reactors fed with 1) 0.7 mM NH2OH only, 2) 0.7 mM NH2OH and 0.5 mM NH4+, and 3) 0.7 mM NH2OH and 0.5 mM NO2–. NH2OH consumption activities were markedly reduced after 7 d of operation, indicating that B. sinica was unable to maintain its activity or biomass by NH2OH disproportionation.
厌氧氨氧化细菌通过NO2 -氧化NH4+产生N2气体,羟胺(NH2OH)是厌氧氨氧化过程的潜在中间体。当厌氧氨氧化菌只与NH2OH孵育时,会产生N2气体,这表明它们具有NH2OH歧化的能力,NH2OH同时是电子的供体和受体。目前关于厌氧氨氧化菌NH2OH歧化的信息有限;因此,本研究采用15n示踪技术对厌氧氨氧化细菌NH2OH歧化的化学计量学进行了研究。将厌氧氨氧化菌、Brocadia sinica、Jettenia caeni和Scalindua sp.添加15NH2OH孵育,并评估15n标记氮化合物的产量。厌氧氨氧化菌进行NH2OH歧化反应,产生15-15N2气和NH4+作为反应产物。作为厌氧氨氧化过程的抑制剂,乙炔的加入降低了NH2OH歧化活性,但不完全降低。在3个上流式厌氧塔式反应器中,分别用1)0.7 mM NH2OH、2)0.7 mM NH2OH和0.5 mM NH4+、3)0.7 mM NH2OH和0.5 mM NO2 -进行氨歧化反应(标准条件下- 240.3 kJ mol NH2OH - 1),考察了水藻的生长情况。运转7 d后,藻体的NH2OH消耗活性显著降低,表明藻体无法通过NH2OH歧化维持活性和生物量。
{"title":"NH2OH Disproportionation Mediated by Anaerobic Ammonium-oxidizing (Anammox) Bacteria","authors":"M. Oshiki, Lin Gao, Lei Zhang, S. Okabe","doi":"10.1264/jsme2.ME21092","DOIUrl":"https://doi.org/10.1264/jsme2.ME21092","url":null,"abstract":"Anammox bacteria produce N2 gas by oxidizing NH4+ with NO2–, and hydroxylamine (NH2OH) is a potential intermediate of the anammox process. N2 gas production occurs when anammox bacteria are incubated with NH2OH only, indicating their capacity for NH2OH disproportionation with NH2OH serving as both the electron donor and acceptor. Limited information is currently available on NH2OH disproportionation by anammox bacteria; therefore, the stoichiometry of anammox bacterial NH2OH disproportionation was examined in the present study using 15N-tracing techniques. The anammox bacteria, Brocadia sinica, Jettenia caeni, and Scalindua sp. were incubated with the addition of 15NH2OH, and the production of 15N-labeled nitrogenous compounds was assessed. The anammox bacteria tested performed NH2OH disproportionation and produced 15-15N2 gas and NH4+ as reaction products. The addition of acetylene, an inhibitor of the anammox process, reduced the activity of NH2OH disproportionation, but not completely. The growth of B. sinica by NH2OH disproportionation (–240.3 kJ mol NH2OH–1 under standard conditions) was also tested in 3 up-flow column anammox reactors fed with 1) 0.7 mM NH2OH only, 2) 0.7 mM NH2OH and 0.5 mM NH4+, and 3) 0.7 mM NH2OH and 0.5 mM NO2–. NH2OH consumption activities were markedly reduced after 7 d of operation, indicating that B. sinica was unable to maintain its activity or biomass by NH2OH disproportionation.","PeriodicalId":18482,"journal":{"name":"Microbes and Environments","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2022-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41463425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
N2O Reduction by Gemmatimonas aurantiaca and Potential Involvement of Gemmatimonadetes Bacteria in N2O Reduction in Agricultural Soils 金双胞菌对农业土壤N2O的还原作用及双胞菌在N2O还原中的潜在作用
IF 2.2 4区 环境科学与生态学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2022-04-12 DOI: 10.1264/jsme2.ME21090
M. Oshiki, Yuka Toyama, T. Suenaga, A. Terada, Y. Kasahara, Takashi Yamaguchi, N. Araki
Agricultural soil is the primary N2O sink limiting the emission of N2O gas into the atmosphere. Although Gemmatimonadetes bacteria are abundant in agricultural soils, limited information is currently available on N2O reduction by Gemmatimonadetes bacteria. Therefore, the effects of pH and temperature on N2O reduction activities and affinity constants for N2O reduction were examined by performing batch experiments using an isolate of Gemmatimonadetes bacteria, Gemmatimonas aurantiaca (NBRC100505T). G. aurantiaca reduced N2O at pH 5–9 and 4–50°C, with the highest activity being observed at pH 7 and 30°C. The affinity constant of G. aurantiaca cells for N2O was 4.4 μM. The abundance and diversity of the Gemmatimonadetes 16S rRNA gene and nosZ encoding nitrous oxide reductase in agricultural soil samples were also investigated by quantitative PCR (qPCR) and amplicon sequencing analyses. Four N2O-reducing agricultural soil samples were assessed, and the copy numbers of the Gemmatimonadetes 16S rRNA gene (clades G1 and G3), nosZ DNA, and nosZ mRNA were 8.62–9.65×108, 5.35–7.15×108, and 2.23–4.31×109 copies (g dry soil)–1, respectively. The abundance of the nosZ mRNA of Gemmatimonadetes bacteria and OTU91, OUT332, and OTU122 correlated with the N2O reduction rates of the soil samples tested, suggesting N2O reduction by Gemmatimonadetes bacteria. Gemmatimonadetes 16S rRNA gene reads affiliated with OTU4572 and OTU3759 were predominant among the soil samples examined, and these Gemmatimonadetes OTUs have been identified in various types of soil samples.
农业土壤是主要的N2O汇,限制了N2O气体排放到大气中。虽然在农业土壤中有大量的双胞菌,但目前关于双胞菌对N2O的还原作用的信息有限。因此,通过对一种双胞菌金双胞菌(Gemmatimonas aurantiaca, NBRC100505T)进行批量实验,研究了pH和温度对N2O还原活性和N2O还原亲和常数的影响。G. aurantiaca在pH值5-9和4-50°C时还原N2O, pH值7和30°C时活性最高。金菖蒲细胞对N2O的亲和力常数为4.4 μM。采用定量PCR (qPCR)和扩增子测序方法,对农业土壤样品中编码氧化亚氮还原酶的gemmatimonadees 16S rRNA基因和nosZ基因的丰度和多样性进行了研究。对4个降低n20的农业土壤样品进行了评价,发现gemmatimonadees 16S rRNA基因(G1和G3枝)、nosZ DNA和nosZ mRNA的拷贝数分别为8.62-9.65×108、5.35-7.15×108和2.23-4.31×109 copies (g dry soil) -1。细菌和OTU91、OUT332、OTU122的nosZ mRNA丰度与土壤样品N2O还原率相关,表明细菌对N2O的还原作用。与OTU4572和OTU3759相关的gemmatimonadees 16S rRNA基因在土壤样品中占主导地位,这些gemmatimonadees OTUs已在不同类型的土壤样品中被鉴定出来。
{"title":"N2O Reduction by Gemmatimonas aurantiaca and Potential Involvement of Gemmatimonadetes Bacteria in N2O Reduction in Agricultural Soils","authors":"M. Oshiki, Yuka Toyama, T. Suenaga, A. Terada, Y. Kasahara, Takashi Yamaguchi, N. Araki","doi":"10.1264/jsme2.ME21090","DOIUrl":"https://doi.org/10.1264/jsme2.ME21090","url":null,"abstract":"Agricultural soil is the primary N2O sink limiting the emission of N2O gas into the atmosphere. Although Gemmatimonadetes bacteria are abundant in agricultural soils, limited information is currently available on N2O reduction by Gemmatimonadetes bacteria. Therefore, the effects of pH and temperature on N2O reduction activities and affinity constants for N2O reduction were examined by performing batch experiments using an isolate of Gemmatimonadetes bacteria, Gemmatimonas aurantiaca (NBRC100505T). G. aurantiaca reduced N2O at pH 5–9 and 4–50°C, with the highest activity being observed at pH 7 and 30°C. The affinity constant of G. aurantiaca cells for N2O was 4.4 μM. The abundance and diversity of the Gemmatimonadetes 16S rRNA gene and nosZ encoding nitrous oxide reductase in agricultural soil samples were also investigated by quantitative PCR (qPCR) and amplicon sequencing analyses. Four N2O-reducing agricultural soil samples were assessed, and the copy numbers of the Gemmatimonadetes 16S rRNA gene (clades G1 and G3), nosZ DNA, and nosZ mRNA were 8.62–9.65×108, 5.35–7.15×108, and 2.23–4.31×109 copies (g dry soil)–1, respectively. The abundance of the nosZ mRNA of Gemmatimonadetes bacteria and OTU91, OUT332, and OTU122 correlated with the N2O reduction rates of the soil samples tested, suggesting N2O reduction by Gemmatimonadetes bacteria. Gemmatimonadetes 16S rRNA gene reads affiliated with OTU4572 and OTU3759 were predominant among the soil samples examined, and these Gemmatimonadetes OTUs have been identified in various types of soil samples.","PeriodicalId":18482,"journal":{"name":"Microbes and Environments","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2022-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42373544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Population Distribution of Phosphate-solubilizing Microorganisms in Agricultural Soil 农业土壤中溶磷微生物的种群分布
IF 2.2 4区 环境科学与生态学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2022-03-26 DOI: 10.1264/jsme2.ME21041
I. Djuuna, S. Prabawardani, Maria Massora
Phosphorus (P) is an essential macronutrient for plant growth and is mainly present in agricultural soil in unavailable forms. Phosphate-solubilizing microorganisms (PSMs) increase soil P availability. The objective of the present study was to assess the population and type of PSMs and their relationships with soil characteristics in the agricultural soil of Manokwari. Twenty-one composite soil samples (0–20 cm) were collected at the rhizospheres of plants in the Prafi and Masni Districts. A dilution technique and plate count method on Pikovskayas agar medium were used to examine the PSM population, phosphate-solubilizing index (PSI), and various soil properties. The results obtained showed that the total population of phosphate-solubilizing bacteria ranged between 25×103 and 550×103 CFU g–1 of soil, while that of phosphate-solubilizing fungi was between 2.0×103 and 5.0×103 CFU g–1 of soil at all locations. The PSI of the isolates ranged between 1.1 to 3.6 mm, with the most efficient and highest PSI being obtained for Bacillus sp. (strain 8) and the lowest for Pseudomonas sp. (strain 15). Six isolates found at all locations were identified at the genus level: Chromobacterium sp., Pseudomonas sp., Bacillus sp., Micrococcus sp., Caulobacter sp., and Aspergillus sp. A correlation was observed between the number of PSMs and the level of soil P availability and moisture content, indicating an increase in soil P availability with a greater abundance of PSMs in soil.
磷(P)是植物生长所必需的大量养分,主要以不可用形式存在于农业土壤中。溶磷微生物(psm)增加土壤磷素有效性。本研究的目的是评估马诺瓦里农业土壤中psm的数量和类型及其与土壤特征的关系。在Prafi和Masni地区的植物根际采集了21份0 ~ 20 cm的复合土壤样品。采用Pikovskayas琼脂培养基稀释法和平板计数法,研究了PSM的数量、磷酸盐溶解指数(PSI)和各种土壤性质。结果表明,各地点土壤增磷菌总数在25×103 ~ 550×103 CFU g-1之间,土壤增磷真菌总数在2.0×103 ~ 5.0×103 CFU g-1之间。分离菌株的PSI范围在1.1 ~ 3.6 mm之间,其中芽孢杆菌(菌株8)的PSI效率最高,假单胞菌(菌株15)的PSI最低。在所有地点均鉴定出属水平的6个分离株:色杆菌、假单胞菌、芽孢杆菌、微球菌、茎状杆菌和曲霉。psm数量与土壤磷有效性和水分含量水平呈相关性,表明土壤中psm丰度越高,土壤磷有效性越高。
{"title":"Population Distribution of Phosphate-solubilizing Microorganisms in Agricultural Soil","authors":"I. Djuuna, S. Prabawardani, Maria Massora","doi":"10.1264/jsme2.ME21041","DOIUrl":"https://doi.org/10.1264/jsme2.ME21041","url":null,"abstract":"Phosphorus (P) is an essential macronutrient for plant growth and is mainly present in agricultural soil in unavailable forms. Phosphate-solubilizing microorganisms (PSMs) increase soil P availability. The objective of the present study was to assess the population and type of PSMs and their relationships with soil characteristics in the agricultural soil of Manokwari. Twenty-one composite soil samples (0–20 cm) were collected at the rhizospheres of plants in the Prafi and Masni Districts. A dilution technique and plate count method on Pikovskayas agar medium were used to examine the PSM population, phosphate-solubilizing index (PSI), and various soil properties. The results obtained showed that the total population of phosphate-solubilizing bacteria ranged between 25×103 and 550×103 CFU g–1 of soil, while that of phosphate-solubilizing fungi was between 2.0×103 and 5.0×103 CFU g–1 of soil at all locations. The PSI of the isolates ranged between 1.1 to 3.6 mm, with the most efficient and highest PSI being obtained for Bacillus sp. (strain 8) and the lowest for Pseudomonas sp. (strain 15). Six isolates found at all locations were identified at the genus level: Chromobacterium sp., Pseudomonas sp., Bacillus sp., Micrococcus sp., Caulobacter sp., and Aspergillus sp. A correlation was observed between the number of PSMs and the level of soil P availability and moisture content, indicating an increase in soil P availability with a greater abundance of PSMs in soil.","PeriodicalId":18482,"journal":{"name":"Microbes and Environments","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2022-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42421486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 10
期刊
Microbes and Environments
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1