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Combinatorial effects of telmisartan and docetaxel on cell viability and metastatic gene expression in human prostate and breast cancer cells 替米沙坦和多烯紫杉醇联合对人前列腺癌和乳腺癌癌症细胞生存能力和转移基因表达的影响
IF 1.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2022-03-01 DOI: 10.22099/mbrc.2022.42638.1700
Marjan Khorsand, Z. Mostafavi-Pour, V. Razban, S. Khajeh, R. Zare
The epithelial-to-mesenchymal transition (EMT) is a unique process resulting in enhanced cell motility, invasiveness, and metastasis in cancer. The EMT is regulated by several transcription factors, including Snail and Slug, which exert crucial roles during cancer progression. We have studied the effects of Docetaxel as the first-line chemotherapy agent for prostate cancer, and Telmisartan as an anti-hypertensive drug on the expression level of Snail and Slug. In addition, the effects of Docetaxel, Telmisartan and their combination on cancer cell proliferation were investigated. The PC3, DU145, MDA-MB468, and HEK cell lines were used for this study. Quantitative RT-PCR analysis and MTT assay were used to study the expression of Snail and Slug level and cell proliferative assay, respectively. We found that a combination of Docetaxel + Telmisartan effectively inhibits the cell proliferation in cancerous cells in comparison with each drug alone (P<0.05). Furthermore, in these cell lines, Docetaxel, Telmisartan and their combination significantly diminished the expression level of Snail and Slug genes compared to control cells (P<0.001), however, in the HEK cell line, this effect was seen only in the combination group. Our data imply that Telmisartan and its combination with Docetaxel exert strong inhibitory effects on the expression level of Snail and Slug genes. Also, these drugs and their combination could inhibit cancer cell proliferation. In conclusion, the combination of Telmisartan and Docetaxel has the potential to suppress the metastasis of prostate and breast cancer cells.
上皮-间质转化(EMT)是一个独特的过程,导致癌症细胞运动性、侵袭性和转移增强。EMT受几种转录因子的调控,包括蜗牛和蛞蝓,它们在癌症进展中起着至关重要的作用。我们研究了多西他赛作为前列腺癌一线化疗药物,替米沙坦作为降压药物对Snail和Slug表达水平的影响。此外,还研究了多西他赛、替米沙坦及其联合用药对肿瘤细胞增殖的影响。本研究采用PC3、DU145、MDA-MB468和HEK细胞系。采用定量RT-PCR法和MTT法分别研究蜗牛和鼻涕虫的表达水平和细胞增殖水平。我们发现多西他赛+替米沙坦联合用药比单独用药更能有效抑制癌细胞的细胞增殖(P<0.05)。此外,在这些细胞系中,与对照细胞相比,多西他赛、替米沙坦及其联合治疗显著降低了Snail和Slug基因的表达水平(P<0.001),然而,在HEK细胞系中,这种影响仅在联合治疗组可见。我们的数据表明替米沙坦及其联合多西他赛对蜗牛和鼻涕虫基因的表达水平有较强的抑制作用。此外,这些药物及其组合可以抑制癌细胞的增殖。综上所述,替米沙坦联合多西他赛具有抑制前列腺和乳腺癌细胞转移的潜力。
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引用次数: 1
Design of a new multi-epitope peptide vaccine for non-small cell Lung cancer via vaccinology methods: an in silico study 通过疫苗学方法设计一种新的非小细胞肺癌多表位肽疫苗:一项计算机研究
IF 1.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2022-03-01 DOI: 10.22099/mbrc.2022.42468.1697
A/Prof. Fatemeh Heidary MD, MPH, FAAO, FICO, M. Tourani, Fatemeh Hejazi-Amiri, S. H. Khatami, N. Jamali, Mortaza Taheri‐Anganeh
Lung cancer is the most common type of tumor worldwide. Non-small-cell lung carcinoma (NSCLC) is considered any epithelial cell-related lung cancer, which includes more than 85% of all lung cancer cases. NSCLC is less responsive to chemotherapy than SCLC. Therefore, the need for other treatments has become more pronounced and immunotherapy has gained increasing attention as a promising therapy in recent years. The current study aimed to design a multi-epitope peptide vaccine targeting main cancer/testis antigens of SP17, AKAP4, and PTTG1, which have a major function in tumor cell proliferation invasion. The protein vaccine was constructed using the rigorous immunoinformatics analysis and investigation of several immune system parameters, considering B cell epitopes and CD4 and CD8 induced epitopes as the most important cells to respond to cancer cells. Inverse translation and optimization of codons were performed to have the designed protein's cloning as well as expression potential in E.coli. Physicochemical, antigenic, and allergenic features were assessed to confirm the safety and immunogenicity of the vaccine. The secondary and tertiary structures were predicted. Finally, intrinsic disorder and 3D model refinement and validation were performed to eliminate structural problems. The designed construct had a stable structure that could be an antigen and stimulate the immune system and not be an allergen. The built model 3D structure was valid and stable. Further investigations are needed to approve the safety and immunogenic property of this new vaccine for NSCLC before it can be used in patients.
肺癌是世界上最常见的肿瘤类型。非小细胞肺癌(NSCLC)被认为是任何上皮细胞相关的肺癌,占所有肺癌病例的85%以上。与SCLC相比,NSCLC对化疗的反应较差。因此,对其他治疗方法的需求变得更加明显,近年来免疫治疗作为一种有前景的治疗方法得到了越来越多的关注。本研究旨在设计一种多表位肽疫苗,靶向在肿瘤细胞增殖侵袭中起主要作用的SP17、AKAP4和PTTG1的主要癌/睾丸抗原。考虑到B细胞表位和CD4和CD8诱导的表位是对癌细胞应答最重要的细胞,通过严格的免疫信息学分析和多个免疫系统参数的研究构建了该蛋白疫苗。通过对密码子的反翻译和优化,验证了所设计蛋白的克隆和在大肠杆菌中的表达潜力。评估了物理化学、抗原和致敏性特征,以确认疫苗的安全性和免疫原性。预测了二级和三级结构。最后,对模型进行内在失序和三维模型精化验证,消除结构问题。所设计的结构具有稳定的结构,可以作为抗原刺激免疫系统,而不是过敏原。所建模型三维结构有效、稳定。在用于非小细胞肺癌患者之前,需要进一步的研究来批准这种新疫苗的安全性和免疫原性。
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引用次数: 1
Recombinase-free cloning (RFC) protocol for gene swapping 用于基因交换的无重组酶克隆(RFC)协议
IF 1.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2022-03-01 DOI: 10.22099/mbrc.2021.41923.1685
Hai-Vy Vo-Nguyen, T. Nguyen, Quoc-Gia Mai, Thien-Thien Tran, T. L. Tran, Hieu Tran‐Van
Recombinant DNA technology has been playing the key role for a long time since its first beginning. DNA ligases have certainly contributed to the development of cloning techniques, as well as molecular study up to now. Despite being a prime cloning tool, DNA ligases still face some shortcomings which lead to their limit of use. Our study provided an improved method that simplified the basic restriction enzyme-based cloning (REC) by eliminating the ligation role, named recombinase-free cloning (RFC). This improved technique was designed with only one PCR reaction, one digestion reaction, and one temperature profile, which takes advantage of endogenous recombinase in E. coli host to create the target recombinant vector inside the cell. All purification steps were eliminated for effectively material- and time-saving. Five different clones were generated by RFC. This method showed relatively low efficiency yet successful at a range of 100% in every conducted trial with fragment sizes from 0.5-1.0 kbp. The RFC method could be completed within a day (about 9 hours), without the need of ligase or recombinase or purification steps, which significantly saved DNA components, materials as well as the time required. In conclusion, we expected to provide a more convenient cloning method, as well as enable faster generation of DNA clones, which would be well applied in the less equipped laboratories.
重组DNA技术自诞生以来一直发挥着关键作用。DNA连接酶无疑为克隆技术的发展以及迄今为止的分子研究做出了贡献。尽管DNA连接酶是一种主要的克隆工具,但它仍然面临着一些缺点,这些缺点导致了它的使用限制。我们的研究提供了一种改进的方法,通过消除连接作用简化了基于基本限制性内切酶的克隆(REC),称为无重组酶克隆(RFC)。这种改进的技术只设计了一个PCR反应、一个消化反应和一个温度曲线,利用大肠杆菌宿主中的内源性重组酶在细胞内产生靶重组载体。为了有效节省材料和时间,取消了所有纯化步骤。RFC生成了五个不同的克隆。该方法显示出相对较低的效率,但在每一次进行的试验中(碎片大小为0.5-1.0kbp)都在100%的范围内成功。RFC方法可以在一天内(约9小时)完成,无需连接酶或重组酶或纯化步骤,这显著节省了DNA成分、材料和所需时间。总之,我们希望提供一种更方便的克隆方法,并能够更快地产生DNA克隆,这将在设备较少的实验室中得到很好的应用。
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引用次数: 2
Effects of trehalose on NFE2L2, catalase, and superoxide dismutase in the kidney of aged rats 海藻糖对衰老大鼠肾脏NFE2L2、过氧化氢酶和超氧化物歧化酶的影响
IF 1.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2022-03-01 DOI: 10.22099/mbrc.2022.42014.1688
Yaser Hozhabri, Asie Sadeghi, Mahdieh Nazari-Robati, Faegheh Bahri, Fouzieh Salimi, M. Abolhassani, A. Mohammadi
Aging is associated with an increase in oxidative stress, which damages organs such as the kidney. Trehalose has abundant beneficial activities including antioxidative effects. This study aimed to investigate the effects of trehalose on several antioxidant parameters of the aged kidney. Wistar rats were divided into three groups: young (4 months), aged (24 months), and aged-trehalose. The third group was treated with 2% trehalose for one month. The expression of target genes and enzyme activities in the kidney of the animals were evaluated by quantitative polymerase chain reaction (qPCR) and enzyme colorimetric procedures, respectively. Protein levels of NFE2L2 showed a 50% reduction in aged rats compared to young rats (P<0.001), which was restored by trehalose intervention. In addition, the activity and mRNA levels of catalase (CAT) increased in aged rats while treatment with trehalose reversed this trend. On the other hand, superoxide dismutase (SOD) activity was reduced in the kidneys of aged rats but was not affected by trehalose intervention .It is concluded that trehalose supplementation alleviates the antioxidant system impairments in the kidneys of aged rats. However, further investigations are needed to thoroughly describe the antioxidative impacts of trehalose on the kidneys during aging.
衰老与氧化应激的增加有关,氧化应激会损害肾脏等器官。海藻糖具有丰富的有益活性,包括抗氧化作用。本研究旨在探讨海藻糖对衰老肾脏若干抗氧化参数的影响。Wistar大鼠分为三组:幼龄(4个月)、老龄(24个月)和老龄海藻糖。第三组用2%海藻糖治疗一个月。分别通过定量聚合酶链式反应(qPCR)和酶比色法评估动物肾脏中靶基因的表达和酶活性。与年轻大鼠相比,老年大鼠的NFE2L2蛋白水平降低了50%(P<0.001),海藻糖干预使其恢复。此外,衰老大鼠的过氧化氢酶(CAT)活性和mRNA水平增加,而海藻糖治疗逆转了这一趋势。另一方面,衰老大鼠肾脏超氧化物歧化酶(SOD)活性降低,但不受海藻糖干预的影响。然而,还需要进一步的研究来彻底描述海藻糖在衰老过程中对肾脏的抗氧化作用。
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引用次数: 1
Toward a better understanding of phylogenetic relationships within Conringieae (Brassicaceae) 更好地了解芸苔科锥属植物的系统发育关系
IF 1.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2022-03-01 DOI: 10.22099/MBRC.2022.42767.1709
A. Khosravi, Atena Eslami-Farouji, Atiqullah Sultani-Ahmadzai, S. Mohsenzadeh
One new tribe (Plagiolobeae), one new species (Plagioloba derakii) together with two new combinations (P. persica and P. clavata) are established within Brassicaceae based on a decisive consideration of molecular phylogenetic dataset, morphological characters, fruit septum nature, as well as seed microsculpturing features. Results distinctly justified Arabis ottonis-schulzii as a synonym of Conringia persica and further molecular analyses proved its placement as a member of genus Plagioloba. It is also placed in a new tribe Plagiolobeae as close relatives of Conringieae and Coluteocarpeae. Finally, the diagnostic morphological characters separating the new tribe from the previously assigned tribe (Conringieae) are also discussed.
基于分子系统发育数据、形态特征、果实隔性质和种子微雕刻特征,在芸苔科中建立了一个新部落(剽窃者)、一个新种(剽窃者)和两个新组合(P. persica和P. clavata)。结果明确证明Arabis ottonis-schulzii是Conringia persica的同义植物,进一步的分子分析也证实了其属于斜齿草属。它也被归入一个新的部落,作为Conringieae和Coluteocarpeae的近亲。最后,对新部落与原部落(Conringieae)的诊断形态学特征进行了讨论。
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引用次数: 5
Identification of Bordetella bronchiseptica in the throat and nose of dogs and cats by PCR. 用聚合酶链反应(PCR)鉴定犬、猫咽喉和鼻腔中的支气管脓毒杆菌。
IF 1.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2022-01-01 DOI: 10.22099/mbrc.2022.43873.1755
Mohammad Tabatabaei, Hamid Reza Rohani

B.bronchiseptica is pathogenic for some domestic and wild animals. Due to the importance of this bacterium, its presence in dogs and cats has been investigated using PCR. Pharyngeal and nasal swabs were taken from 135 dogs and 42 cats. Based on the PCR performed on the dogs' samples, in 25/63 (39.68%) pharyngeal samples and 20/59 (33.89%) nasal samples DNA of B. bronchiseptica detected. On the other hand, according to the PCR performed on the cats' samples, in 9/23 (39.13%) pharyngeal samples and 319 (15.78%) nasal samples DNA of B. bronchiseptica was existed. According to the present study, the rate of B. bronchiseptica infection is high among dogs and cats in Iran. Also, due to the fact that the prevalence of this bacterium among pets animals is not exactly known in Iran, necessary measures should be taken for rapid diagnosis and treatment and proper control of the infection.

支杆菌对一些家畜和野生动物具有致病性。由于这种细菌的重要性,它在狗和猫的存在已经用PCR进行了调查。采集了135只狗和42只猫的咽拭子和鼻拭子。对犬标本进行PCR检测,25/63(39.68%)的咽部标本和20/59(33.89%)的鼻腔标本中检出支气管杆菌DNA。另一方面,对猫标本进行PCR检测,9/23只(39.13%)猫咽标本和319只(15.78%)猫鼻标本中存在支杆菌DNA。根据目前的研究,伊朗的狗和猫的支杆菌感染率很高。此外,由于伊朗宠物动物中这种细菌的流行情况尚不清楚,因此应采取必要措施,迅速诊断和治疗并适当控制感染。
{"title":"Identification of <i>Bordetella bronchiseptica</i> in the throat and nose of dogs and cats by PCR.","authors":"Mohammad Tabatabaei,&nbsp;Hamid Reza Rohani","doi":"10.22099/mbrc.2022.43873.1755","DOIUrl":"https://doi.org/10.22099/mbrc.2022.43873.1755","url":null,"abstract":"<p><p><i>B.bronchiseptica</i> is pathogenic for some domestic and wild animals. Due to the importance of this bacterium, its presence in dogs and cats has been investigated using PCR. Pharyngeal and nasal swabs were taken from 135 dogs and 42 cats. Based on the PCR performed on the dogs' samples, in 25/63 (39.68%) pharyngeal samples and 20/59 (33.89%) nasal samples DNA of <i>B. bronchiseptica</i> detected. On the other hand, according to the PCR performed on the cats' samples, in 9/23 (39.13%) pharyngeal samples and 319 (15.78%) nasal samples DNA of <i>B. bronchiseptica</i> was existed. According to the present study, the rate of <i>B. bronchiseptica</i> infection is high among dogs and cats in Iran. Also, due to the fact that the prevalence of this bacterium among pets animals is not exactly known in Iran, necessary measures should be taken for rapid diagnosis and treatment and proper control of the infection.</p>","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"11 3","pages":"127-131"},"PeriodicalIF":1.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9661675/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10592096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genomic analysis of Enterococcus durans NT21, a putative bacteriocin-producing isolate. 推测产细菌素分离物杜兰肠球菌NT21的基因组分析。
IF 1.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2022-01-01 DOI: 10.22099/mbrc.2022.44088.1760
Nashwa Tarek, Ahmed O El-Gendy, Ahmed S Khairalla, Medhat Abdel-Fattah, Eman Tawfik, Ahmed F Azmy

Enterococcus species are a long-standing and non-pathogenic commensal bacterium, representing an important part of the normal. Enterococcus durans is a rarely isolated species from animals and humans, and it was a tiny constituent of human oral cavity and animal intestinal flora, as well as animal-derived foods, particularly dairy products. This study evaluated the security of our strain E. durans NT21 by using whole-genome sequencing (WGS), physicochemical features, and antimicrobial activity. The complete genomic of our strain Enterococcus durans NT21was sequenced and analyzed by using several bioinformatics tools to identify bacteriocin genes, virulence genes, antibiotic resistance genes, Crispr-Cas and pathogenicity islands. The results showed that our strain NT21 lacks the presence of virulence genes, pathogenicity islands, plasmids and has only two antibiotic resistance genes. On the other hand, it produces three bacteriocin-like inhibitory substances (Enterolysin A, P and L50a). It has six gene-encoded Crisper-Cas and one cluster Crispr-Cas gene. According to our findings, E. durans NT21 is a possible probiotic strain that is safe for both human and animal use.

肠球菌是一种存在已久的非致病性共生细菌,是正常肠球菌的重要组成部分。杜兰肠球菌是一种很少从动物和人类中分离出来的物种,它是人类口腔和动物肠道菌群以及动物源性食品,特别是乳制品的微小组成部分。本研究通过全基因组测序(WGS)、理化特性和抗菌活性等指标对菌株NT21的安全性进行了评价。利用多种生物信息学工具对我国产杜兰肠球菌nt21进行了全基因组测序和分析,鉴定了细菌素基因、毒力基因、抗生素耐药基因、Crispr-Cas和致病性岛。结果表明,菌株NT21缺乏毒力基因、致病性岛、质粒,仅有2个耐药基因。另一方面,它产生三种细菌素样抑制物质(肠溶素A、P和L50a)。它有六个基因编码的Crispr-Cas和一个集群Crispr-Cas基因。根据我们的研究结果,durans NT21可能是一种对人类和动物都安全的益生菌菌株。
{"title":"Genomic analysis of <i>Enterococcus durans</i> NT21, a putative bacteriocin-producing isolate.","authors":"Nashwa Tarek,&nbsp;Ahmed O El-Gendy,&nbsp;Ahmed S Khairalla,&nbsp;Medhat Abdel-Fattah,&nbsp;Eman Tawfik,&nbsp;Ahmed F Azmy","doi":"10.22099/mbrc.2022.44088.1760","DOIUrl":"https://doi.org/10.22099/mbrc.2022.44088.1760","url":null,"abstract":"<p><p><i>Enterococcus</i> species are a long-standing and non-pathogenic commensal bacterium, representing an important part of the normal. <i>Enterococcus durans</i> is a rarely isolated species from animals and humans, and it was a tiny constituent of human oral cavity and animal intestinal flora, as well as animal-derived foods, particularly dairy products. This study evaluated the security of our strain <i>E. durans</i> NT21 by using whole-genome sequencing (WGS), physicochemical features, and antimicrobial activity. The complete genomic of our strain <i>Enterococcus durans</i> NT21was sequenced and analyzed by using several bioinformatics tools to identify bacteriocin genes, virulence genes, antibiotic resistance genes, Crispr-Cas and pathogenicity islands. The results showed that our strain NT21 lacks the presence of virulence genes, pathogenicity islands, plasmids and has only two antibiotic resistance genes. On the other hand, it produces three bacteriocin-like inhibitory substances (Enterolysin A, P and L50a). It has six gene-encoded Crisper-Cas and one cluster Crispr-Cas gene. According to our findings, <i>E. durans</i> NT21 is a possible probiotic strain that is safe for both human and animal use.</p>","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"11 3","pages":"143-153"},"PeriodicalIF":1.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9661671/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10646859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Correlation between adiponectin rs2241766 and rs266729 polymorphisms and risk of papillary thyroid cancer. 脂联素rs2241766和rs266729多态性与甲状腺乳头状癌风险的相关性
IF 1.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2022-01-01 DOI: 10.22099/mbrc.2022.43012.1714
Mohsen Maleki, Mansour Karajibani, Mohsen Sarvani, Farzaneh Montazerifar, Saeedeh Salimi, Zahra Heidari

About 60-80% of thyroid cancer (TC) cases are papillary thyroid cancer (PTC). Studies have shown that serum adiponectin levels are inversely related to the risk of TC and PTC. Aim of the present study was to evaluate the association between adiponectin rs2241766 and rs266729 polymorphisms and risk of PTC. 122 PTC patients and 128 healthy subjects were enrolled in the study. PCR-RFLP and ARMS-PCR methods were used for genotype analysis. The rs266729 polymorphism did not correlate with risk of PTC. As regard rs2241766 polymorphism, the frequency of the GG genotype did not have a significant difference between the two groups, although, PTC cases showed higher frequency of GT genotype compared to controls (OR=2.87, 95% CI=1.56-5.28, P=0.001). We observed a significant association between adiponectin rs2241766 polymorphism and PTC, however, our result showed no significant relationship between adiponectin rs266729 polymorphism and risk of PTC.

约60-80%的甲状腺癌是乳头状甲状腺癌(PTC)。研究表明,血清脂联素水平与TC和PTC的风险呈负相关。本研究的目的是评估脂联素rs2241766和rs266729多态性与PTC风险的关系。122名PTC患者和128名健康受试者参加了这项研究。采用PCR-RFLP和ARMS-PCR方法进行基因型分析。rs266729多态性与PTC发病风险无相关性。rs2241766多态性方面,两组间GG基因型频率无显著差异,但PTC病例GT基因型频率高于对照组(OR=2.87, 95% CI=1.56 ~ 5.28, P=0.001)。我们观察到脂联素rs2241766多态性与PTC有显著相关性,但我们的结果显示脂联素rs266729多态性与PTC的风险无显著相关性。
{"title":"Correlation between adiponectin rs2241766 and rs266729 polymorphisms and risk of papillary thyroid cancer.","authors":"Mohsen Maleki,&nbsp;Mansour Karajibani,&nbsp;Mohsen Sarvani,&nbsp;Farzaneh Montazerifar,&nbsp;Saeedeh Salimi,&nbsp;Zahra Heidari","doi":"10.22099/mbrc.2022.43012.1714","DOIUrl":"https://doi.org/10.22099/mbrc.2022.43012.1714","url":null,"abstract":"<p><p>About 60-80% of thyroid cancer (TC) cases are papillary thyroid cancer (PTC). Studies have shown that serum adiponectin levels are inversely related to the risk of TC and PTC. Aim of the present study was to evaluate the association between adiponectin rs2241766 and rs266729 polymorphisms and risk of PTC. 122 PTC patients and 128 healthy subjects were enrolled in the study. PCR-RFLP and ARMS-PCR methods were used for genotype analysis. The rs266729 polymorphism did not correlate with risk of PTC. As regard rs2241766 polymorphism, the frequency of the GG genotype did not have a significant difference between the two groups, although, PTC cases showed higher frequency of GT genotype compared to controls (OR=2.87, 95% CI=1.56-5.28, P=0.001). We observed a significant association between adiponectin rs2241766 polymorphism and PTC, however, our result showed no significant relationship between adiponectin rs266729 polymorphism and risk of PTC.</p>","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"11 3","pages":"113-118"},"PeriodicalIF":1.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9661673/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10592099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Stress adaptation signature into the functional units of spike, envelope, membrane protein and ssRNA of SARS-CoV-2. SARS-CoV-2刺突、包膜、膜蛋白和ssRNA功能单元的胁迫适应特征
IF 1.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2022-01-01 DOI: 10.22099/mbrc.2022.44594.1777
Aniket Sarkar, Anindya Sundar Panja

Pandemic coronavirus causes respiratory, enteric and sometimes neurological diseases. Proteome data of individual coronavirus strains were already reported. Here we investigated of SARS-CoV-2 ssRNA and protein of spike, envelope and membrane to determine stress adaptation profile. Thermodynamic properties, Physicochemical behaviour and, amino acid composition along with their RMSD value was analysed. Thermodynamic index of SARS-CoV2 spike, envelope and membrane ssRNA is unstable in higher temperature. Presence of higher proportion of polar with positive and negative charged amino acid residues into spike (S), envelope (E) and membrane (M) protein indicate the lower stress adaptability pattern. Our study represented several unstable pockets into S, E and M proteins of SARS-CoV-2 against different abiotic stresses, specifically higher in spike protein. Contact with heat through solvent may denature the architectural network of SARS-CoV-2 spike, envelope and membrane ssRNA and structural protein. The stress instability index of SARS-CoV-2 and the interactome profile of its transmembrane proteins may help to reveal novel factors for inhibiting SARS-CoV-2 growth.

大流行冠状病毒会引起呼吸道、肠道疾病,有时还会引起神经系统疾病。个别冠状病毒株的蛋白质组数据已经报道。本文研究了SARS-CoV-2刺突、包膜和膜的ssRNA和蛋白,以确定胁迫适应谱。对其热力学性质、理化性质、氨基酸组成及其RMSD值进行了分析。SARS-CoV2刺突、包膜和膜ssRNA的热力学指标在高温下不稳定。穗蛋白(S)、包膜蛋白(E)和膜蛋白(M)中极性带正电荷和负电荷的氨基酸残基比例较高,表明胁迫适应模式较低。我们的研究表明,针对不同的非生物胁迫,SARS-CoV-2的S、E和M蛋白中存在几个不稳定的口袋,特别是刺突蛋白含量较高。通过溶剂与热接触可使SARS-CoV-2刺突、包膜和膜ssRNA和结构蛋白的结构网络变性。SARS-CoV-2的胁迫不稳定性指数及其跨膜蛋白的相互作用谱可能有助于揭示抑制SARS-CoV-2生长的新因子。
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引用次数: 0
Phylogenetic analysis and prevalence of Delta hepatitis among HBsAg carriers in Afghanistan. 阿富汗乙肝表面抗原携带者丁型肝炎的系统发育分析和流行情况。
IF 1.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2022-01-01 DOI: 10.22099/mbrc.2022.44692.1780
Abbas Ali Husseini, Mehran Rostamzadeh

The molecular profile of hepatitis Delta in Afghanistan remains unclear yet, therefore this study addresses the genotype of HDV among HBsAg carriers in Afghanistan. In total 234 HBsAg-positive sera were examined by chemiluminescent micro-particle immunoassay to detect Anti-HDV antibodies. Serologically positive samples were later approved via real-time PCR test and subsequently, a 731 bp segment of the HDV Delta antigen RNA region was sequenced in the Illumina platform. The isolates were genotyped via distance matrix/UPGMA analysis using Kimura 2-parameter by MEGA7 software package program. The HBV/HDV coinfection rate among HBsAg carriers in Afghanistan was 2.1%. Finally, 4 samples successfully amplified Hepatitis delta antigen (HDAg) which Later in phylogenetic analysis, all resided in branch genotype I and were stored at GenBank with accession numbers MK799645, MK799646, MK799647, MK799648. The HDV genotypic variations in the Afghan HBsAg carriers may be homogenous and HDV-1 may be the predominant genotype in Afghanistan.

阿富汗丁型肝炎的分子特征尚不清楚,因此本研究解决了阿富汗HBsAg携带者中HDV的基因型问题。采用化学发光微粒子免疫法检测234份hbsag阳性血清的抗hdv抗体。血清学阳性样品随后通过实时PCR检测获得批准,随后在Illumina平台上对HDV δ抗原RNA区域的731 bp片段进行测序。利用MEGA7软件包程序,采用距离矩阵/UPGMA分析,采用木村2参数进行基因分型。阿富汗HBsAg携带者中HBV/HDV合并感染率为2.1%。最终,4份样本成功扩增出丁型肝炎抗原(HDAg),经系统发育分析,均为分支基因型I,保存于GenBank,登录号为MK799645、MK799646、MK799647、MK799648。阿富汗乙肝表面抗原携带者的HDV基因型变异可能是同质的,HDV-1可能是阿富汗的主要基因型。
{"title":"Phylogenetic analysis and prevalence of Delta hepatitis among HBsAg carriers in Afghanistan.","authors":"Abbas Ali Husseini,&nbsp;Mehran Rostamzadeh","doi":"10.22099/mbrc.2022.44692.1780","DOIUrl":"https://doi.org/10.22099/mbrc.2022.44692.1780","url":null,"abstract":"<p><p>The molecular profile of hepatitis Delta in Afghanistan remains unclear yet, therefore this study addresses the genotype of HDV among HBsAg carriers in Afghanistan. In total 234 HBsAg-positive sera were examined by chemiluminescent micro-particle immunoassay to detect Anti-HDV antibodies. Serologically positive samples were later approved via real-time PCR test and subsequently, a 731 bp segment of the HDV Delta antigen RNA region was sequenced in the Illumina platform. The isolates were genotyped via distance matrix/UPGMA analysis using Kimura 2-parameter by MEGA7 software package program. The HBV/HDV coinfection rate among HBsAg carriers in Afghanistan was 2.1%. Finally, 4 samples successfully amplified Hepatitis delta antigen (HDAg) which Later in phylogenetic analysis, all resided in branch genotype I and were stored at GenBank with accession numbers MK799645, MK799646, MK799647, MK799648. The HDV genotypic variations in the Afghan HBsAg carriers may be homogenous and HDV-1 may be the predominant genotype in Afghanistan.</p>","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"11 4","pages":"183-186"},"PeriodicalIF":1.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9905752/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10707069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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Molecular Biology Research Communications
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