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Neurochemical aspects of post-tetanic potentiation of monosynaptic reflexes in the cat spinal cord. II. Determination of phospholipids at maximum of potentiation. 猫脊髓单突触反射破伤风后增强的神经化学方面。2最大增强时磷脂含量的测定。
Neurobiology
Pub Date : 1975-03-01
H H Althaus, V Neuhoff

A monosynaptic reflex pathway was used to produce a post-tetanic potentiation (PTP). in ten experiments (cats) one side was tetanized (via N. gastrocnemius) whereas the other one was taken as control. Tissue was punched out of the ventral horn area of the spinal cord (segment height L7/S1) for the analysis of the phospholipid content. The results demonstrate that PTP significantly increases phosphatidyl-inositol in the potentiated alpha-motoneurone area. Phosphatidylserine showed a trend towards a decrease; sphingomyelin, phosphatidylcholine and phosphatidylethanolamine remained almost unchanged. The effect of a different tetanizing time on the phospholipid content is discussed as is the intention of the present experiments.

单突触反射通路产生破伤风后增强(PTP)。在10个实验(猫)中，一侧(通过腓肠肌绦虫)进行破伤风，而另一侧作为对照组。从脊髓前角区(节段高度L7/S1)取出组织进行磷脂含量分析。结果表明，PTP显著增加了增强的α -运动神经元区域的磷脂酰肌醇。磷脂酰丝氨酸呈下降趋势;鞘磷脂、磷脂酰胆碱和磷脂酰乙醇胺基本保持不变。讨论了不同破伤风时间对磷脂含量的影响，这是本实验的目的。
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引用次数: 0
Serotonin binding proteins from human blood platelets. An experimental model system for studies on properties of synaptic vesicles. 人血小板中的血清素结合蛋白。突触囊泡性质研究的实验模型系统。
Neurobiology
Pub Date : 1975-03-01
P F Pignatti, L L Cavalli-Sforza

Serotonin uptake, binding and release were studied in human blood platelets. The uptake showed marked individual differences while the loss of 5-HT during resuspension of the 5-HT loaded platelets was constant. The existence of 5-HT binding proteins was demonstrated by equilibrium dialysis, gel filtration on a column equilibrated with 5-HT, affinity chromatography on a 5-HT-Sepharose column and polyacryl-amide disc gel electrophoresis experiments. After incubation of the platelet extract in the presence of [14-C]5-HT, electrophoretic and autoradiographic analysis demonstrated the presence of three 5-HT binding proteins, two of which were soluble glycoproteins specific for serotonin.

研究了血清素在人血小板中的吸收、结合和释放。摄取表现出明显的个体差异，而负载5-HT的血小板重悬过程中5-HT的损失是恒定的。通过平衡透析、5-HT平衡柱上的凝胶过滤、5-HT- sepharose柱上的亲和层析和聚丙烯酰胺圆盘凝胶电泳实验，证实了5-HT结合蛋白的存在。将血小板提取物在[14-C]5-羟色胺中孵育后，电泳和放射自显影分析显示存在三种5-羟色胺结合蛋白，其中两种是血清素特异性的可溶性糖蛋白。
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引用次数: 0
Induced differentiation of clonal rat nerve and glial cells. 克隆大鼠神经和胶质细胞的诱导分化。
Neurobiology
Pub Date : 1974-01-01
D Schubert
{"title":"Induced differentiation of clonal rat nerve and glial cells.","authors":"D Schubert","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":19126,"journal":{"name":"Neurobiology","volume":"4 6","pages":"376-87"},"PeriodicalIF":0.0,"publicationDate":"1974-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15555159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Kinetics of choline uptake in mixed neuronal-glial, and exclusively glial cultures. 混合神经元-胶质细胞和纯胶质细胞培养中胆碱摄取动力学。
Neurobiology
Pub Date : 1974-01-01
R Massarelli, M Sensenbrenner, A Ebel, P Mandel
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引用次数: 0
A dissociated cell culture bioassay for nerve growth factor. 神经生长因子的分离细胞培养生物测定。
Neurobiology
Pub Date : 1974-01-01
L A Greene
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引用次数: 0
Rotation cultures from different regions of embryonic chick brain. I. Biochemical and morphological characteristics. 鸡胚脑不同区域的旋转培养。1 .生物化学和形态特征。
Neurobiology
Pub Date : 1974-01-01
G R Peterson, P H Fischer, A Burkhalter
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引用次数: 0
A comparative morphological study of the developing node-paranode region in lumbar spinal roots. II. Light microscopy after osmiumtetroxide-alpha-naphthylamine (OTAN)-staining. 腰椎根淋巴结-旁阳极区发育的比较形态学研究。2四氧化锇- α -萘胺(OTAN)染色后的光镜。
Neurobiology
Pub Date : 1974-01-01
C H Berthold
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引用次数: 0
Indirect Wallerian degeneration in intramedullary root fibers of the kitten hypoglossal nerve light and electron microscopical observations on silver impregnated sections. 小猫舌下神经髓内根纤维的间接沃勒氏变性。
Neurobiology
Pub Date : 1974-01-01
H Aldskogius
{"title":"Indirect Wallerian degeneration in intramedullary root fibers of the kitten hypoglossal nerve light and electron microscopical observations on silver impregnated sections.","authors":"H Aldskogius","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":19126,"journal":{"name":"Neurobiology","volume":"4 3","pages":"132-50"},"PeriodicalIF":0.0,"publicationDate":"1974-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15246910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A comparative morphological study of the developing node-paranode region in lumbar spinal roots. I. Electron microscopy. 腰椎根淋巴结-旁阳极区发育的比较形态学研究。1 .电子显微镜。
Neurobiology
Pub Date : 1974-01-01
C H Berthold
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引用次数: 0
Kinetic properties of the GABA uptake system in cultures of chick retina. 鸡视网膜GABA摄取系统的动力学特性。
Neurobiology
Pub Date : 1974-01-01
G Tunnicliff, Y D Cho, R O Martin
{"title":"Kinetic properties of the GABA uptake system in cultures of chick retina.","authors":"G Tunnicliff,&nbsp;Y D Cho,&nbsp;R O Martin","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":19126,"journal":{"name":"Neurobiology","volume":"4 1","pages":"38-42"},"PeriodicalIF":0.0,"publicationDate":"1974-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15244364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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