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Phytopathology Research最新文献

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The phytopathogen Xanthomonas campestris scavenges hydroxycinnamic acids in planta via the hca cluster to increase virulence on its host plant 植物病原黄单胞菌通过hca簇清除植物体内的羟基肉桂酸，增加对寄主植物的毒力
IF 3.4 2区 农林科学 Q2 PLANT SCIENCES
Phytopathology Research
Pub Date : 2022-04-20 DOI: 10.1186/s42483-022-00119-z
Bo Chen, Rui-Fang Li, Lian Zhou, Kai Song, A. Poplawsky, Ya-Wen He
{"title":"The phytopathogen Xanthomonas campestris scavenges hydroxycinnamic acids in planta via the hca cluster to increase virulence on its host plant","authors":"Bo Chen, Rui-Fang Li, Lian Zhou, Kai Song, A. Poplawsky, Ya-Wen He","doi":"10.1186/s42483-022-00119-z","DOIUrl":"https://doi.org/10.1186/s42483-022-00119-z","url":null,"abstract":"","PeriodicalId":20098,"journal":{"name":"Phytopathology Research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2022-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45148909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Molecular characterization of a novel Conyza canadensis-infecting begomovirus in China 一种新的感染中国秋海棠的加拿大稻的分子特征
IF 3.4 2区 农林科学 Q2 PLANT SCIENCES
Phytopathology Research
Pub Date : 2022-04-19 DOI: 10.1186/s42483-022-00118-0
Pengbai Li, Ke Li, C. Jing, Rui Wu, Gentu Wu, Mingjun Li, L. Qing
{"title":"Molecular characterization of a novel Conyza canadensis-infecting begomovirus in China","authors":"Pengbai Li, Ke Li, C. Jing, Rui Wu, Gentu Wu, Mingjun Li, L. Qing","doi":"10.1186/s42483-022-00118-0","DOIUrl":"https://doi.org/10.1186/s42483-022-00118-0","url":null,"abstract":"","PeriodicalId":20098,"journal":{"name":"Phytopathology Research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2022-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47624751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
The hypervariable N-terminal of soybean mosaic virus P1 protein influences its pathogenicity and host defense responses 大豆花叶病毒P1蛋白高变n端影响其致病性和宿主防御反应
IF 3.4 2区 农林科学 Q2 PLANT SCIENCES
Phytopathology Research
Pub Date : 2022-04-06 DOI: 10.1186/s42483-022-00115-3
C. Mao, Shiqi Shan, Yue Huang, C. Jiang, Hehong Zhang, Yanjun Li, Jianping Chen, Zhongyan Wei, Zongtao Sun
{"title":"The hypervariable N-terminal of soybean mosaic virus P1 protein influences its pathogenicity and host defense responses","authors":"C. Mao, Shiqi Shan, Yue Huang, C. Jiang, Hehong Zhang, Yanjun Li, Jianping Chen, Zhongyan Wei, Zongtao Sun","doi":"10.1186/s42483-022-00115-3","DOIUrl":"https://doi.org/10.1186/s42483-022-00115-3","url":null,"abstract":"","PeriodicalId":20098,"journal":{"name":"Phytopathology Research","volume":"4 1","pages":""},"PeriodicalIF":3.4,"publicationDate":"2022-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65793856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Identification and characterization of a stem canker and twig dieback disease of pear caused by Neofusicoccum parvum in Chinese mainland 中国大陆梨茎腐病的鉴定与鉴定
IF 3.4 2区 农林科学 Q2 PLANT SCIENCES
Phytopathology Research
Pub Date : 2022-03-15 DOI: 10.1186/s42483-022-00111-7
Feng He, Jie Yang, Yan-cun Zhao, P. Laborda, Yifan Jia, Asma Safdar, A. Kange, Bingxin Li, Lan Zhou, Q. Zeng, Sally Brown, Z. Fu, Fengquan Liu
{"title":"Identification and characterization of a stem canker and twig dieback disease of pear caused by Neofusicoccum parvum in Chinese mainland","authors":"Feng He, Jie Yang, Yan-cun Zhao, P. Laborda, Yifan Jia, Asma Safdar, A. Kange, Bingxin Li, Lan Zhou, Q. Zeng, Sally Brown, Z. Fu, Fengquan Liu","doi":"10.1186/s42483-022-00111-7","DOIUrl":"https://doi.org/10.1186/s42483-022-00111-7","url":null,"abstract":"","PeriodicalId":20098,"journal":{"name":"Phytopathology Research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2022-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44819062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
The Pseudomonas syringae effector AvrPtoB targets abscisic acid signaling pathway to promote its virulence in Arabidopsis 丁香假单胞菌效应物AvrPtoB靶向脱落酸信号通路，提高拟南芥毒力
IF 3.4 2区 农林科学 Q2 PLANT SCIENCES
Phytopathology Research
Pub Date : 2022-03-01 DOI: 10.1186/s42483-022-00110-8
Yan-zhi Liu, Md. Rubel Mahmud, N. Xu, Jun Liu
{"title":"The Pseudomonas syringae effector AvrPtoB targets abscisic acid signaling pathway to promote its virulence in Arabidopsis","authors":"Yan-zhi Liu, Md. Rubel Mahmud, N. Xu, Jun Liu","doi":"10.1186/s42483-022-00110-8","DOIUrl":"https://doi.org/10.1186/s42483-022-00110-8","url":null,"abstract":"","PeriodicalId":20098,"journal":{"name":"Phytopathology Research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44398387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Ubiquitination in the rice blast fungus Magnaporthe oryzae: from development and pathogenicity to stress responses 稻瘟病菌的泛素化:从发育、致病性到胁迫反应
IF 3.4 2区 农林科学 Q2 PLANT SCIENCES
Phytopathology Research
Pub Date : 2022-01-12 DOI: 10.1186/s42483-021-00106-w
Yu Wang, Nan Yang, Yunna Zheng, Jiaolin Yue, V. Bhadauria, You-Liang Peng, Qianwang Chen
{"title":"Ubiquitination in the rice blast fungus Magnaporthe oryzae: from development and pathogenicity to stress responses","authors":"Yu Wang, Nan Yang, Yunna Zheng, Jiaolin Yue, V. Bhadauria, You-Liang Peng, Qianwang Chen","doi":"10.1186/s42483-021-00106-w","DOIUrl":"https://doi.org/10.1186/s42483-021-00106-w","url":null,"abstract":"","PeriodicalId":20098,"journal":{"name":"Phytopathology Research","volume":"4 1","pages":""},"PeriodicalIF":3.4,"publicationDate":"2022-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65793806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
Reverse transcription-recombinase-aided amplification and CRISPR/Cas12a-based visual detection of maize chlorotic mottle virus. 逆转录重组酶辅助扩增及基于CRISPR/ cas12的玉米变色斑驳病毒视觉检测
IF 3.4 2区 农林科学 Q2 PLANT SCIENCES
Phytopathology Research
Pub Date : 2022-01-01 Epub Date: 2022-06-20 DOI: 10.1186/s42483-022-00128-y
Xueyan Duan, Wendi Ma, Zhiyuan Jiao, Yiying Tian, Ragab Gomaa Ismail, Tao Zhou, Zaifeng Fan

Maize chlorotic mottle virus (MCMV) is one of the important quarantine pathogens in China. It often co-infects with one or two viruses in the family Potyviridae and causes maize lethal necrosis disease. Therefore, an accurate and sensitive method for the detection of MCMV is urgently needed. Combined with reverse transcription and recombinase-aided amplification, we developed a CRISPR/Cas12a-based visual nucleic acid detection system targeting the MCMV coat protein gene. The whole process can be completed within 45 min with high sensitivity. This system could detect cDNAs diluted up to 10-5 when 2000 ng of total RNA was used for reverse transcription. The Cas12a/crRNA complex designed for MCMV detection could recognize and cleave the targeted double-stranded DNA, and ultimately cleave the single-stranded DNA probes and produce fluorescent signals. The green fluorescence produced under blue light (440-460 nm) in this procedure could be observed by the naked eye. Since this novel method is specific, rapid, sensitive and does not require special instruments and technical expertise, it should be suitable for on-site visual detection of MCMV in seeds, plants of maize and potentially in its insect vectors.

玉米褪绿斑驳病毒(MCMV)是中国重要的检疫病原菌之一。它经常与波蒂病毒科的一种或两种病毒共同感染，引起玉米致命坏死病。因此，迫切需要一种准确灵敏的MCMV检测方法。结合逆转录和重组酶辅助扩增技术，我们开发了一种基于CRISPR/ cas12的靶向MCMV外壳蛋白基因的视觉核酸检测系统。整个过程可在45分钟内完成，灵敏度高。当总RNA浓度为2000 ng时，该系统可以检测到稀释至10-5的dna。设计用于MCMV检测的Cas12a/crRNA复合体能够识别并切割目标双链DNA，最终切割单链DNA探针并产生荧光信号。在蓝光(440-460 nm)下产生的绿色荧光可以用肉眼观察到。该方法具有特异、快速、灵敏等特点，且不需要特殊仪器和专业技术，适用于玉米种子、植株及其潜在病媒MCMV的现场视觉检测。
{"title":"Reverse transcription-recombinase-aided amplification and CRISPR/Cas12a-based visual detection of maize chlorotic mottle virus.","authors":"Xueyan Duan,&nbsp;Wendi Ma,&nbsp;Zhiyuan Jiao,&nbsp;Yiying Tian,&nbsp;Ragab Gomaa Ismail,&nbsp;Tao Zhou,&nbsp;Zaifeng Fan","doi":"10.1186/s42483-022-00128-y","DOIUrl":"https://doi.org/10.1186/s42483-022-00128-y","url":null,"abstract":"<p><p>Maize chlorotic mottle virus (MCMV) is one of the important quarantine pathogens in China. It often co-infects with one or two viruses in the family <i>Potyviridae</i> and causes maize lethal necrosis disease. Therefore, an accurate and sensitive method for the detection of MCMV is urgently needed. Combined with reverse transcription and recombinase-aided amplification, we developed a CRISPR<b>/</b>Cas12a-based visual nucleic acid detection system targeting the MCMV coat protein gene. The whole process can be completed within 45 min with high sensitivity. This system could detect cDNAs diluted up to 10<sup>-5</sup> when 2000 ng of total RNA was used for reverse transcription. The Cas12a/crRNA complex designed for MCMV detection could recognize and cleave the targeted double-stranded DNA, and ultimately cleave the single-stranded DNA probes and produce fluorescent signals. The green fluorescence produced under blue light (440-460 nm) in this procedure could be observed by the naked eye. Since this novel method is specific, rapid, sensitive and does not require special instruments and technical expertise, it should be suitable for on-site visual detection of MCMV in seeds, plants of maize and potentially in its insect vectors.</p>","PeriodicalId":20098,"journal":{"name":"Phytopathology Research","volume":"4 1","pages":"23"},"PeriodicalIF":3.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9207886/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40401949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 9
Evaluation of potato virus X resistance in potato cultivars and identification of an innate immunity-independent resistance phenotype 马铃薯品种对X病毒抗性的评价及先天免疫非依赖性抗性表型的鉴定
IF 3.4 2区 农林科学 Q2 PLANT SCIENCES
Phytopathology Research
Pub Date : 2021-09-13 DOI: 10.1186/s42483-021-00099-6
Jiahui Liu, Ye Liu, Yue Fang, Lili Zhang, Kai-Kang Yu, Xiaoyun Wu, Xiaofei Cheng
{"title":"Evaluation of potato virus X resistance in potato cultivars and identification of an innate immunity-independent resistance phenotype","authors":"Jiahui Liu, Ye Liu, Yue Fang, Lili Zhang, Kai-Kang Yu, Xiaoyun Wu, Xiaofei Cheng","doi":"10.1186/s42483-021-00099-6","DOIUrl":"https://doi.org/10.1186/s42483-021-00099-6","url":null,"abstract":"","PeriodicalId":20098,"journal":{"name":"Phytopathology Research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2021-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45061691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Species and genetic variability of sweet potato viruses in China 中国甘薯病毒的种类和遗传变异
IF 3.4 2区 农林科学 Q2 PLANT SCIENCES
Phytopathology Research
Pub Date : 2021-08-30 DOI: 10.1186/s42483-021-00097-8
Yongjiang Wang, Yanhong Qin, Shuang Wang, Desheng Zhang, Y. Tian, F. Zhao, Yingzhi Wang, Hui Lv, Q. Qiao, Zhenchen Zhang
{"title":"Species and genetic variability of sweet potato viruses in China","authors":"Yongjiang Wang, Yanhong Qin, Shuang Wang, Desheng Zhang, Y. Tian, F. Zhao, Yingzhi Wang, Hui Lv, Q. Qiao, Zhenchen Zhang","doi":"10.1186/s42483-021-00097-8","DOIUrl":"https://doi.org/10.1186/s42483-021-00097-8","url":null,"abstract":"","PeriodicalId":20098,"journal":{"name":"Phytopathology Research","volume":"3 1","pages":""},"PeriodicalIF":3.4,"publicationDate":"2021-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65793748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Transcription factor UvMsn2 is important for vegetative growth, conidiogenesis, stress response, mitochondrial morphology and pathogenicity in the rice false smut fungus Ustilaginoidea virens 转录因子UvMsn2在水稻假黑穗病真菌的营养生长、分生、胁迫反应、线粒体形态和致病性等方面具有重要意义
IF 3.4 2区 农林科学 Q2 PLANT SCIENCES
Phytopathology Research
Pub Date : 2021-07-31 DOI: 10.1186/s42483-021-00093-y
Yuandi Xu, Shuang Wu, Zhaomeng Yu, Emily Kolojane Moeketsi, Zhixiang Yang, Zhengguang Zhang, Haifeng Zhang
{"title":"Transcription factor UvMsn2 is important for vegetative growth, conidiogenesis, stress response, mitochondrial morphology and pathogenicity in the rice false smut fungus Ustilaginoidea virens","authors":"Yuandi Xu, Shuang Wu, Zhaomeng Yu, Emily Kolojane Moeketsi, Zhixiang Yang, Zhengguang Zhang, Haifeng Zhang","doi":"10.1186/s42483-021-00093-y","DOIUrl":"https://doi.org/10.1186/s42483-021-00093-y","url":null,"abstract":"","PeriodicalId":20098,"journal":{"name":"Phytopathology Research","volume":"3 1","pages":""},"PeriodicalIF":3.4,"publicationDate":"2021-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s42483-021-00093-y","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65793677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
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