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THE EFFECT OF ADDING LICORICE EXTRACT ON PRODUCTIVE PERFORMANCE OF LACTATING ZARAIBI GOATS 添加甘草提取物对泌乳扎拉比山羊生产性能的影响
Pub Date : 2023-09-01 DOI: 10.21608/ejas.2023.330987
Walaa M. Abd El-Wahab, M. I. Ahmed, M. E. El-Kolany, E. M. El-Kotamy, S. M. Shedeed
The purpose of this study was to determine how the use of licorice extracts (LE) as feed additives improved the production of dairy Zaraibi goats. Based on their milk production, animal weight, and age, twenty lactating Zaraibi goats were classified into four comparable groups, each consisting of five animals (Av. body weight, 39.1±2.5 kg). Each animal group received one of the following experimental ration at random treatments: T1 (control ration): consisted of 60% concentrate feed mixture (CFM) + 20% berseem hay (BH) + 20% Rice straw (RS) and without additives and all experimental treatments T2, T3 and T4 which, Animals were fed, the control ration plus 2, 4 and 6 g /head/day of the LE as fed supplement, respectively. The experimental rations included according to NRC (2007) . Numerically, the T1 group consumed feed more (P< 0.05) than the other groups. However, there were no significant (P>0.05) changes in TDN, DCP and water intakes among all treatments. No significant changes in values of digestibilities, TDN, and DCP among all experimental rations. All tested levels of LE resulted in insignificant increases in milk yield, but it caused significant (P< 0.05) amelioration in feed utilization efficiency values in comparison with the control. Goats supplemented with the 4 g LE/h/d in T3 had the greatest (P< 0.05) values of feed utilization efficiency, while those in T1 recorded the least values. Animals receiving 6g/head/day of LE (T4) had significantly higher levels of fat, protein, and galactose in milk compared to those in T3, while T1 had the lowest values. However, no significant difference was observed in milk total solids, ash, and lactose percentages. The LE did not significantly affect the measured blood parameters. The T4 ration recorded the highest relative economic efficiency at 217.19%, compared to the control ration 100%. It could be concluded that the LE led to beneficial effects on the productive performance of the lactating Zaraibi goats.
本研究旨在确定使用甘草提取物(LE)作为饲料添加剂如何提高扎莱比奶山羊的产量。根据产奶量、体重和年龄,20 只泌乳扎莱比山羊被分为四个可比组,每组 5 只(平均体重 39.1±2.5 公斤)。每组动物随机接受以下一种实验饲粮:T1(对照日粮):由 60% 的混合精饲料(CFM)+ 20% 的青贮干草(BH)+ 20% 的稻草(RS)组成,不含任何添加剂;T2、T3 和 T4 为所有实验处理,分别饲喂对照日粮和 2、4 和 6 克/头/天的 LE 作为饲料添加剂。实验日粮包括 NRC(2007 年)规定的日粮。从数值上看,T1 组的饲料消耗量比其他组多(P< 0.05)。然而,所有处理的 TDN、DCP 和水摄入量均无明显变化(P>0.05)。所有试验日粮的消化率、TDN 和 DCP 值均无明显变化。与对照组相比,所有试验水平的 LE 均未显著提高产奶量,但显著提高了饲料利用效率(P< 0.05)。在 T3 中,补充 4 克 LE/h/d 的山羊的饲料利用效率值最大(P< 0.05),而在 T1 中,补充 4 克 LE/h/d 的山羊的饲料利用效率值最小。与 T3 组相比,每天添加 6 克 LE 的动物(T4)牛奶中的脂肪、蛋白质和半乳糖含量明显更高,而 T1 组的含量最低。然而,在牛奶总固体、灰分和乳糖百分比方面没有观察到明显差异。LE 对测量的血液参数没有明显影响。T4 日粮的相对经济效益最高,为 217.19%,而对照日粮为 100%。由此可以得出结论,LE 对泌乳扎拉比山羊的生产性能产生了有益的影响。
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引用次数: 0
PEDALING EXERCISE AS A REHABILITATION FOR CHILDREN WITH CEREBRAL PALSY (A REVIEW ARTICLE) 踩踏运动作为脑瘫儿童的康复手段(综述文章)
Pub Date : 2023-09-01 DOI: 10.21608/ejas.2023.328990
Alaa B.M. Hasan, Nanees E. Mohamed, Amira F. El-Sheikh
Cerebral palsy (CP) is a common cause of disability during childhood. It is a neuromuscular development disorder that is caused by a non-progressive impairment to the fetal brain. There are many neuromuscular, musculoskeletal and developmental impairments associated with CP, including spasticity, dystonia, contractures, abnormal bone marrow development, balance disturbances, selective loss of motor control, and weakness. Recently. There has been increased focus on participation in physical activity in children with CP. Exercises that help people with spasticity include cycling, strengthening exercises and treadmill training . The cycle ergometer can be used in cases with CP to improve cardiovascular fitness, balance, gait and upper limbs function. The goal of this review was to present cycling as rehabilitation tool and its effect in children with CP.
脑瘫(CP)是造成儿童残疾的常见原因。它是一种神经肌肉发育障碍,由胎儿大脑的非进行性损伤引起。与 CP 相关的神经肌肉、肌肉骨骼和发育障碍有很多,包括痉挛、肌张力障碍、挛缩、骨髓发育异常、平衡障碍、选择性运动控制能力丧失和虚弱。最近人们越来越重视让患有 CP 的儿童参加体育活动。对痉挛患者有帮助的运动包括骑自行车、强化训练和跑步机训练。自行车测力计可用于改善 CP 患儿的心血管健康、平衡、步态和上肢功能。本综述旨在介绍作为康复工具的自行车运动及其对脊髓灰质炎儿童的影响。
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引用次数: 0
THE EFFECTS OF INTERMITTENT FASTING WITH HIGH FAT/ LOW CARB DIET ON OBESE RATS 间歇性禁食和高脂肪/低碳水化合物饮食对肥胖大鼠的影响
Pub Date : 2023-09-01 DOI: 10.21608/ejas.2023.328988
Dina S. Abou-Bakr, Maysa M. El-Malah, Hany G. EL-Masry
Intermittent fasting (IF) has an increasingly role of lose weight in overweight and normal weight individuals. The present study was investigated that the effects of intermittent fasting with high fat/low carb diet on obese rats. Forty-two adult male albino rats weighting (185± 10 g) were randomly divided into 6 equal groups (n=7). Group 1 was fed on basal diet (as negative control group). Group 2 was fed on basal diet and fasted (as positive control group). Groups of rats (3:6) were fasted 24 hours for 3 nonconsecutive day/week. Group 3 was fed on 20%, 20%, and 60% of energy from fat, protein, and carbohydrate, respectively. Group 4 was fed on 30%, 20%, 50% of energy from fat, protein, and carbohydrate, respectively, group 5 was fed on 40%, 20%, 40% of energy from fat, protein, and carbohydrate, respectively, group 6 was fed on 50%, 20%, 30% of energy from fat, protein, and carbohydrate, respectively. Rats weighed twice a week and weight gain was calculated. At the last week of the feeding trial, 3 rats from each group were injected using 0.1 ml formalin (4%) to induce inflammation. At the end of experimental period (8 weeks) the rats were euthanized and blood samples were withdrawn for separating the serum were collected for biochemical analysis. Peritoneal fat pad was dissected and weighed. Blood glucose level, insulin concentration, leptin concentration, lipid profile (TC, TG, HDL-c, LDL-c and VLDL-c), liver functions (AST and ALT) and kidney functions (creatinine and uric acid) were determined. The results showed that fasting (24 h of fasting nonconsecutive day/week
间歇性禁食(IF)对超重和正常体重者的减肥作用越来越大。本研究调查了间歇性禁食与高脂肪/低碳水化合物饮食对肥胖大鼠的影响。将 42 只体重为(185± 10 g)的成年雄性白化大鼠随机分为 6 组(n=7)。第 1 组以基础饮食喂养(作为阴性对照组)。第 2 组喂食基础食物并禁食(作为阳性对照组)。各组大鼠(3:6)连续 3 天/周禁食 24 小时。第 3 组分别摄入 20%、20% 和 60% 的脂肪、蛋白质和碳水化合物。第 4 组分别从脂肪、蛋白质和碳水化合物中摄入 30%、20% 和 50%的能量,第 5 组分别从脂肪、蛋白质和碳水化合物中摄入 40%、20% 和 40%的能量,第 6 组分别从脂肪、蛋白质和碳水化合物中摄入 50%、20% 和 30%的能量。大鼠每周称重两次,并计算体重增长。在喂食试验的最后一周,每组各给 3 只大鼠注射 0.1 ml 福尔马林(4%)以诱发炎症。实验结束时(8 周),对大鼠实施安乐死,抽取血样分离血清进行生化分析。剖开腹膜脂肪垫并称重。测定血糖水平、胰岛素浓度、瘦素浓度、血脂谱(TC、TG、HDL-c、LDL-c 和 VLDL-c)、肝功能(AST 和 ALT)和肾功能(肌酐和尿酸)。结果显示,空腹(24 小时非连续空腹/周
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引用次数: 0
EFFECTS OF VARIETIES, SOWING DATES, AND PLANTING DISTANCES ON VICIA FABA L., YIELD AND GREEN SEEDS CONTAINS OF THE TYROSINE. 品种、播种日期和播种距离对紫花苜蓿产量和绿色种子中酪氨酸含量的影响。
Pub Date : 2023-09-01 DOI: 10.21608/ejas.2023.330989
H. H. A. El-Nour, Max Min
Two faba bean varieties i. e., Nubaria-1, and Spanish were sown in different cultivated dates 15 th of October, November, and December during winter seasons of 2020/2021 and 2021/2022 at different planting distances (30, 25, and 15cm) in sandy loam soil at the experimental farm of El-kassasein Station, Ismailia governorate, Horticulture Research Institute, Agriculture, Research Center, Egypt .The effects of previous factors on the vegetative growth, yield components and green seeds contents of protein, carbohydrates and amino acid (Tyrosine) which is the generator of constructor L-DOPA compound that treated of Parkinsons disease (PD). The results showed that Nubaria-1 obtained augment vegetative growth (plant length, plant fresh and dry weight), yield attributes (number of pods/plant and seeds dry weight percentage), and seeds chemical components percentage (protein, and tyrosine). Meanwhile, Spanish variety was surpassing in number of branches/plant, 100-seed weight, number of seeds/pod, and seeds contents of carbohydrates. Sowing faba bean in 15 th November was the optimum date to produce greatest plant growth, green pods, green seeds yield/fed., and pods characteristic as well as seeds chemical contents of carbohydrates, protein, and amino acid (Tyrosine). Planting distances at of 25cm resulted in vigor plant growth then in second order the distance at 30 cm between hills. While, the superiority values of number of pods/plant, 100-seed weight and dry seed weight percentage as well as green seeds chemical contents were established according to space 30cm apart between plants. Conclusively, the interaction between variety Nubaria-1, sowing in 15 th November, and planting distance at 30cm increased the chemical components percentage of tyrosine and protein.
两个蚕豆品种,即在 2020/2021 年和 2021/2022 年冬季的 10 月 15 日、11 月 15 日和 12 月 15 日,在埃及伊斯梅利亚省农业研究中心园艺研究所 El-kassasein 站试验农场的沙壤土中,以不同的播种距离(30 厘米、25 厘米和 15 厘米)播种了 Nubaria-1 和西班牙。研究前述因素对植物生长、产量成分和绿色种子中蛋白质、碳水化合物和氨基酸(酪氨酸)含量的影响。结果表明,Nubaria-1 提高了无性系生长(植株长度、植株鲜重和干重)、产量属性(荚数/株和种子干重百分比)和种子化学成分百分比(蛋白质和酪氨酸)。同时,西班牙品种在分枝数/株、100 粒种子重量、种子数/荚、种子碳水化合物含量方面均超过了其他品种。11 月 15 日播种蚕豆是最适宜的播种期,可获得最佳的植株生长、绿色豆荚、绿色种子产量/喂入量、豆荚特征以及种子中碳水化合物、蛋白质和氨基酸(酪氨酸)的化学含量。种植间距为 25 厘米时,植株生长旺盛,其次是种植间距为 30 厘米时。植株间距为 30 厘米时,荚果数/株、100 粒种子重量和干种子重量百分比以及绿色种子化学成分含量都较高。最后,品种 Nubaria-1、11 月 15 日播种和 30 厘米种植间距之间的相互作用提高了酪氨酸和蛋白质的化学成分百分比。
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引用次数: 0
EFFECT OF RHIZOBACTERIA AND MINERAL FERTILIZATION ON YIELD AND ITS COMPONENTS OF MELILOTUS ELEGANS PLANTS UNDER CONDITIONS OF EL-HAMAM AREA – EGYPT 根瘤菌和矿物质肥料对埃及哈马姆地区旱生三叶草产量及其成分的影响
Pub Date : 2023-09-01 DOI: 10.21608/ejas.2023.325866
Amal E. Ahmed
The aim of this study was approach a maximum yield of Melilotus plants by integration between bio-fertilizers and mineral fertilizers especially P fertilizers. A field experiment was conducted for two successive years (2018 and 2019) using completely randomized field experiments with three replications for each treatment in El-Hamam area, Marsa Matrouh Governorate, Egypt (between the intersection of the longitude 30º 34' 51'' N and the altitude 30º 15' 40'' E). Melilotus elegans was the investigated crop, sown in the in plots (3×4m) in rows. Biofertilization treatments were Rhizobia, phosphate dissolving bacteria ( Bacillus megatherium and Pseudomonas putida ). The mineral fertilization was applied as a general treatment using three rates of 15 , 30 and 45 kgP 2 O 5 /fed. as calcium super phosphate(15.5%P 2 O 5 ) mixed with the soil during soil preparation . N and K fertilizers were added at one rate of 80 kg N/fed. as NH 4 NO 3 and 40 kg K 2 O as potassium sulphate divided into two equal doses applied at seedling and after cut one stages. The dose of 10m 3 organic manure was added. Obtained results clearly showed that, mixed biofertilization treatment recorded highest values for yield and its components as well as total N,P and oil % in content of shoot and leaves of Melilotus plant for two cuts and during two growing seasons followed by P.putida , and then PDB while mineral P increase yield parameters with increase rates of P up to P3. From the obtained results we can cocluded that, mixed biofertilization treatment combined with P fertilization was superior treatment for Melilotus plants under El-Hamam soil conditions .
本研究的目的是通过整合生物肥料和矿物肥料(尤其是磷肥),实现美兰木植物的最高产量。在埃及 Marsa Matrouh 省 El-Hamam 地区(位于北纬 30º 34' 51'' 和东经 30º 15' 40'' 的交点之间)连续两年(2018 年和 2019 年)进行了田间试验,采用完全随机田间试验,每个处理有三个重复。所研究的作物为旱生黑麦草(Melilotus elegans),在小区(3×4 米)中成行播种。生物施肥处理包括根瘤菌、磷酸盐溶解菌(巨型芽孢杆菌和假单胞菌)。矿物肥料作为一般处理,采用 15、30 和 45 千克过磷酸钙(15.5%P 2 O 5)的三种施肥量,在整地时与土壤混合。氮肥和钾肥的施用量分别为 80 千克氮(以 NH 4 NO 3 计)/饲料和 40 千克 K 2 O(以硫酸钾计)/饲料,分为苗期和割后两个阶段施用。此外,还添加了 10m 3 的有机肥。结果清楚地表明,混合生物肥料处理在两个生长季中的两次砍伐中记录了最高的产量及其成分,以及甜叶菊嫩枝和叶片的总氮、总磷和含油量,其次是普氏菌,然后是PDB。从获得的结果中我们可以得出结论,在哈马姆土壤条件下,混合生物肥料处理与钾肥处理相结合,对美乐托植物来说是更优的处理方法。
{"title":"EFFECT OF RHIZOBACTERIA AND MINERAL FERTILIZATION ON YIELD AND ITS COMPONENTS OF MELILOTUS ELEGANS PLANTS UNDER CONDITIONS OF EL-HAMAM AREA – EGYPT","authors":"Amal E. Ahmed","doi":"10.21608/ejas.2023.325866","DOIUrl":"https://doi.org/10.21608/ejas.2023.325866","url":null,"abstract":"The aim of this study was approach a maximum yield of Melilotus plants by integration between bio-fertilizers and mineral fertilizers especially P fertilizers. A field experiment was conducted for two successive years (2018 and 2019) using completely randomized field experiments with three replications for each treatment in El-Hamam area, Marsa Matrouh Governorate, Egypt (between the intersection of the longitude 30º 34' 51'' N and the altitude 30º 15' 40'' E). Melilotus elegans was the investigated crop, sown in the in plots (3×4m) in rows. Biofertilization treatments were Rhizobia, phosphate dissolving bacteria ( Bacillus megatherium and Pseudomonas putida ). The mineral fertilization was applied as a general treatment using three rates of 15 , 30 and 45 kgP 2 O 5 /fed. as calcium super phosphate(15.5%P 2 O 5 ) mixed with the soil during soil preparation . N and K fertilizers were added at one rate of 80 kg N/fed. as NH 4 NO 3 and 40 kg K 2 O as potassium sulphate divided into two equal doses applied at seedling and after cut one stages. The dose of 10m 3 organic manure was added. Obtained results clearly showed that, mixed biofertilization treatment recorded highest values for yield and its components as well as total N,P and oil % in content of shoot and leaves of Melilotus plant for two cuts and during two growing seasons followed by P.putida , and then PDB while mineral P increase yield parameters with increase rates of P up to P3. From the obtained results we can cocluded that, mixed biofertilization treatment combined with P fertilization was superior treatment for Melilotus plants under El-Hamam soil conditions .","PeriodicalId":201881,"journal":{"name":"Egyptian Journal of Applied Science","volume":"51 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139346582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
OPTIMIZING NITROGEN FERTILIZERS BY USING SOME STIMULANTS FOR IMPROVING YIELD AND QUALITY OF TRITICUM ASTIVUM L. UNDER MINIA GOVERNORATE CONDITIONS 在米尼亚省条件下,通过使用一些刺激剂优化氮肥,以提高 Triticum astivum L. 的产量和质量
Pub Date : 2023-09-01 DOI: 10.21608/ejas.2023.330839
B. Rashwan, M. El-Azazy, Asmaa S. Abd, M. A. Nasef
A field experiment was conducted during two successive winter growing seasons of (2021/ 2022 and 2022/ 2023) at the experimental farm of Mallawi Agriculture Research Station, Minia Governorate, Egypt. The objective of this study was to illustrate the integrated effect of the mineral nitrogen fertilization and stimulant substances on wheat plants. A split-plot design was used, the main plot was stimulant substances (without, amino acids, seaweed extract and mix of amino acids plus seaweed extract). While the nitrogen fertilization (60%, 80% and 100% from recommended dose (RD), were assigned in sub-plot. The results showed that in most traits there was a significant affect interaction between stimulants and N fertilizer treatments. The highest grain yield (9.490, 9.455 ton ha -1 ) was obtained by treating wheat with combination of amino acids plus seaweed extract with 80% of Nitrogen Recommended Dose NRD in both seasons of study, respectively. In addition, the increase in mineral contents and protein contents of wheat grain through the same treatment.
在埃及米尼亚省马拉维农业研究站的实验农场,于 2021/ 2022 年和 2022/ 2023 年连续两个冬季生长季节进行了一项田间试验。这项研究的目的是说明矿物氮肥和刺激物质对小麦植株的综合影响。研究采用了分小区设计,主小区为刺激物质(无、氨基酸、海藻提取物和氨基酸加海藻提取物的混合物)。而氮肥施用量(推荐剂量的 60%、80% 和 100%)则被分配到子小区。结果表明,在大多数性状上,刺激剂和氮肥处理之间存在显著的交互影响。在研究的两个季节中,分别以 80% 的氮推荐剂量 NRD 处理氨基酸加海藻提取物组合的小麦获得了最高的谷物产量(9.490、9.455 吨公顷-1)。此外,通过相同的处理,小麦籽粒的矿物质含量和蛋白质含量也有所增加。
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引用次数: 0
EFFECT OF INOCULATION WITH N2 FIXERS, MICRONUTRIENTS AND INORGANIC N ON COUNTS OF AZOSPIRILLUM WITH RHIZOSPHERE OF BARLEY PLANT IN SOUTH SINAI AREA- EGYPT 接种氮固定剂、微量营养元素和无机氮对埃及西奈半岛南部大麦根瘤菌数量的影响
Pub Date : 2023-07-01 DOI: 10.21608/ejas.2023.325863
Amal E. Ahmed
In order to study the effect of bio-fertilizers, micronutrients and N mineral application on barley plant under calcareous soil conditions. Field experiment was conducted at Experimental Research Station –Ras Sudr, D.R.C. in 2017-and 2018 winter seasons. Experimental factors were: micronutrients as foliar application (Fe, Mn Zn at 250 ppm), mineral N (50, 75 and100 kg N/fed) and Bio-fertilizers (control, Azotobacter chroococcum and Azospirillum brasilense either single or mixed application). The obtained results found that, significant effects of either bio-fertilizers, mineral N and micronutrients as foliar application on yield parameters, mineral contents of barley plants, yield and its attributes, P and N mineral contents of barley grains and microbial activity in barley plant rhizosphere. Interaction of mixed bio-fertilizers treatment micronutrients foliar application of 250 ppm gave maximum enhancement for most studied treatments. Barley straw and grains yield during the two successive seasons. While P, N contents of both barley shoots and grains yield recorded maximum values with mixed bio-fertilizers treatment, mineral N and Micronutrients as foliar application of 250 ppm. The most effect treatment for microbial counts of bio - fertilizers, yield parameters and nutrients content was Mixture bio-fertilizers Mineral N at 100 units/fed and Mocronutrients 250ppm.
为了研究在石灰性土壤条件下施用生物肥料、微量营养元素和矿物质氮对大麦植株的影响。2017年和2018年冬季,在特克斯和凯科斯群岛拉斯苏德试验研究站进行了田间试验。实验因素包括:叶面喷施微量营养元素(250 ppm的铁、锰、锌)、矿物氮(50、75和100 kg N/喂料)和生物肥料(对照、褐藻酸氮菌和巴西褐藻酸氮菌单施或混施)。结果发现,叶面喷施生物肥料、矿物氮和微量营养元素对产量参数、大麦植株的矿物含量、产量及其属性、大麦籽粒的磷和氮矿物含量以及大麦植株根瘤中的微生物活动都有显著影响。混合生物肥料处理微量营养元素叶面喷施 250ppm 的交互作用对大多数研究处理都有最大的提高。连续两季的大麦秸秆和谷物产量。混合生物肥料、矿物质氮和微量营养元素叶面喷施 250 ppm,大麦嫩芽和谷物中的磷、氮含量均达到最高值。对生物肥料微生物数量、产量参数和养分含量影响最大的处理是混合生物肥料矿物质氮(100 单位/次)和微量营养元素(250ppm)。
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引用次数: 0
AN INNOVATIVE APPROACH USING LYTIC PHAGE MIX FOR WASTEWATER MANAGEMENT AND PATHOGEN CONTROL 一种利用溶解噬菌体混合物进行废水管理和病原体控制的创新方法
Pub Date : 2023-03-01 DOI: 10.21608/ejas.2023.310295
Mohamed I. Azzam, Abeer A. Faiesal, Fafy A. Mohammed, A. Korayem
In the modern water management plans of nations that confront a severe lack of water resources, such as the Middle Eastern countries, the reuse of treated sewage water for agriculture is seen as an important alternative water supply. The goal of the current study was to use a lytic phage mixture to reduce both of Escherichia coli strains, coliforms, and other Enterobacteriaceae species in River Nile and drains outlets. The transmission electron microscope revealed morphological similarities between three novel phages (MCn4, MCn5, and MCn6) and those in the Siphoviridae, Myoviridae, and Podoviridae families. Escherichia coli ATCC® strain 11775 and Escherichia coli ATCC® strain 10536, the index coliform, were both lysed by these new phages. Different DNA polymerase (DP), tail protein (TP), and DNA polymerase accessory (DPA) gene sizes (2565 bp, 672 bp, and 951 bp, respectively) were reported by bioinformatics analyses, and all nucleotide sequences were recorded in the international GenBank. Phage-mediated bio-control in aquatic pathogenic bacteria relies on targeting various species and strains, with polyvalent MCn4, MCn5, and MCn6 phages exhibiting lytic effects on Salmonella typhi, Proteus vulgaris, and Citrobacter freundii strains. These phages had a significant lytic influence on the population of coliform bacteria after two hours of incubation. The study concluded that the use of this lytic coliphage mixtures for the decrease in coliform populations in sewage may be considered an efficient and cost-effective alternative to the expensive replacement of wastewater treatment plant Egypt. J. of Appl. Sci., 38 (3-4) 2023 14-44
在中东国家等水资源严重缺乏的国家的现代水管理计划中,将处理过的污水重新用于农业被视为一种重要的替代水供应。本研究的目的是使用一种溶解噬菌体混合物来减少尼罗河和排水口中的大肠杆菌菌株、大肠菌群和其他肠杆菌科物种。透射电镜显示,三种新型噬菌体(MCn4、MCn5和MCn6)与Siphoviridae、Myoviridae和Podoviridae科的噬菌体形态相似。大肠埃希菌ATCC®菌株11775和大肠埃希菌ATCC®菌株10536均被这些新的噬菌体裂解。生物信息学分析报告了不同DNA聚合酶(DP)、尾蛋白(TP)和DNA聚合酶附件(DPA)基因大小(分别为2565 bp、672 bp和951 bp),并将所有核苷酸序列记录在国际GenBank中。噬菌体介导的水生致病菌的生物防治依赖于针对不同的物种和菌株,多价MCn4、MCn5和MCn6噬菌体对伤寒沙门氏菌、普通变形杆菌和弗氏柠檬酸杆菌菌株具有裂解作用。这些噬菌体在孵育两小时后对大肠菌群的种群有显著的裂解作用。该研究的结论是,使用这种溶解噬菌体混合物来减少污水中的大肠菌群可能被认为是一种有效和具有成本效益的替代方案,可以替代昂贵的埃及污水处理厂。J.苹果公司。科学。, 38 (3-4) 2023 14-44
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引用次数: 0
SENSORY AND BIOLOGICAL EVALUATION OF CHIA AND QUINOA- FORTIFIED GLUTEN FREE-BISCUIT PRODUCTS ON CELIAC DISEASE IN RATS 奇亚藜和藜麦强化无麸质饼干产品对大鼠腹腔疾病的感官和生物学评价
Pub Date : 2023-03-01 DOI: 10.21608/ejas.2023.304654
Marwa A. El-Said, Maysa M. El-Mallah, M. Shalaby, H. Elmasry
Celiac disease (CD) is a chronic inflammatory disease of the upper small intestine triggered by gluten protein intolerance, which is prevalent in genetically predisposed persons. The present study was aimed to explore the sensory and biological evaluation of chia and quinoa-fortified gluten - free biscuit products on CD in rats. Sensory evaluation of prepared biscuit products supplemented with chia, quinoa and their combination was performed. Results revealed that incorporation of chia and quinoa flour with rice flour improved sensory properties of prepared biscuit products. Thirty-five male Sprague Dawley rats were used and allocated into five equal groups; Group1: negative control, group 2: positive control with CD and groups 3, 4 and 5: with CD and were fed on chia, quinoa and their combination, respectively. Feed intake (FI), body weight gain (BWG %), and feed efficiency ratio (FER) were calculated. Blood samples were collected for determination of parameters of liver & kidney function, total cholesterol (TC) , triglyceride (TG) , lipoprotein fractions, glutathione (GSH), malondialdehyde (MDA) and lactate dehydrogenase (LDH). Histopathology of small intestine was also carried out. Feeding of chia, quinoa and their combination to rats decreased BWG%, FER, TC, TG and parameters of hepatorenal function. Histopathological examination of small intestine demonstrated that feeding combination of chia and quinoa ameliorated lesions of CD and intestinal inflammation. It could be concluded that fortification of rice flour with quinoa and chia flour improve the sensory properties of prepared biscuit products and when fed to rats may be effective as curative for celiac disease
乳糜泻(CD)是一种由麸质蛋白不耐受引起的上小肠慢性炎症性疾病,在遗传易感人群中普遍存在。本研究旨在探讨奇亚麦和藜麦强化无麸质饼干产品对大鼠CD的感官和生物学评价。对添加奇亚藜、藜麦及其组合的预制饼干产品进行了感官评价。结果表明,在米粉中掺入奇亚藜麦粉可改善饼干制品的感官性能。选取35只雄性斯普拉格·道利大鼠,分为5组;1组为阴性对照组,2组为CD阳性对照组,3、4、5组为CD阳性对照组,分别饲喂奇亚麦、藜麦及其组合饲料。计算采食量(FI)、体增重(BWG %)和饲料效率(FER)。采集血样,测定肝肾功能、总胆固醇(TC)、甘油三酯(TG)、脂蛋白组分、谷胱甘肽(GSH)、丙二醛(MDA)、乳酸脱氢酶(LDH)等指标。同时进行小肠组织病理学检查。饲喂奇亚藜、藜麦及其组合可降低大鼠体重增重%、总脂肪、总脂肪、总甘油三酯及肝肾功能指标。小肠组织病理学检查表明,奇亚藜和藜麦组合喂养改善了CD病变和肠道炎症。由此可见,在米粉中添加藜麦粉和奇亚粉可以改善饼干制品的感官特性,并可用于大鼠的腹腔疾病治疗
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引用次数: 0
INFLUENCE OF SOME FACTORS ON SUSCEPTIBILITY OF TWELVE VIRULENT ISOLATES OF RALSTONIA SOLANACEARUM TO FIVE NANOPARTICLES 几种因素对茄枯病菌12株毒力分离株对5种纳米粒子敏感性的影响
Pub Date : 2023-03-01 DOI: 10.21608/ejas.2023.311901
Amira Rabea, E. Naeem, N. Balabel, M. S. Hanafy, Ghadir E. Daigham
Overall objective of the present study was to evaluate presence of pathogenic Ralstonia solanacearum isolates, the causative agent of wilt disease, which isolated from different sources in four Egyptian Goverovernorate. In addition, optimizing the environmental conditions necessary for the antibacterial activities of five nanoparticles In Vitro trials against the isolated pathogen isolates. The results indicated that, the highest bacterial incidence (87.2 %) was detected in the samples of El-Behira Governorate, while, the lowest (26.7 %) was recorded in samples collected from El-Ismaelia Governorate. Only twelve isolates were virulent and subjected to further studies. The highest severity of disease incidence was recorded with (R9) isolate. Identification of the isolates from soil, irrigation water, tubers and weeds were carried out by immunofluorescent antibody staining of the pathogenic isolates which showed short rod shaped stained as bright green fluorescent in IF test confirmed that these isolates belong to R. solanacearum . Results of Polymerase Chain Reaction (PCR) technique on the twelve isolates, which gave the highest virulence showed that the samples visualize specific 718 bp PCR product under UV light pointing to the very close similarity between the twelve isolates tested of R. solanacearum . No variation could be detected as well in ( Taq -Man) determination, Taq - Man is more specific test used to detect races and biovars of R. solanacearum by using reference bacteria
本研究的总体目的是评估从埃及4个省不同来源分离的枯萎病病原枯枯菌的存在。此外,优化了5种纳米颗粒对分离的病原菌的体外抑菌试验所需的环境条件。结果显示,El-Behira省的细菌感染率最高(87.2%),El-Ismaelia省的细菌感染率最低(26.7%)。只有12株毒株具有毒性,有待进一步研究。(R9)分离株的发病率最高。对土壤、灌溉水、块茎和杂草中分离的病原菌进行免疫荧光抗体染色,IF试验显示短棒状呈亮绿色荧光,证实这些分离的病原菌属于茄青霉。对12株毒力最高的菌株进行PCR检测,结果表明,在紫外光照射下,12株菌株的PCR产物特异性为718 bp,表明12株菌株的毒力非常接近。(Taq -Man)检测结果无明显差异,Taq -Man是一种以参比菌为检测标准菌种和生物变种的特异性较强的检测方法
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引用次数: 0
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Egyptian Journal of Applied Science
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