In primary maize roots (cv. LG 11) endogenous indol-3yl-acetic acid (IAA), measured by gas chromatography-mass spectrometry (GC-MS) technique, was predominantly located in the stele. When these roots were placed for 2 h in horizontal position and in white light, the IAA level was slightly but significantly enhanced in the lower part of the cortex of the elongation zone.
Cross-reactivity of 6 monoclonal anti-rye phytochrome antibodies (mAR1–6) and 6 monoclonal anti-pea phytochrome antibodies (mAP1–6) with ‘large’ phytchromes extracted from etiolated tissues of duckweed, rice, rye, oat, radish, soybean, mung bean and pea was examined by radioimmunoassay using sheep red blood cells coupled to rye or pea phytochrome as target cells. Oat phytochrome reacted with only mAR5 and mAR6. Radish phytochrome reacted with mAP6, and soybean phytochrome reacted with mAP5 and mAP6. Mung bean phytochrome reacted with mAP4, mAP5 and mAP6. No cross-reactions were observed with any other possible combinations except those of mARs with antigen rye phytochrome and of mAPs with pea phytochrome. These results are discussed in terms of the sites on phytochrome molecules which are recognized by these monoclonal antibodies.
Remarkable yields of highly dividing protoplasts were isolated from rootlets (60-h-old) and mesophyll of Medicago arborea L. Plating efficiency was satisfactory for root and excellent for leaf protoplasts. Culture conditions were established for callus induction and growth from various organ explants and plant regeneration. Fertile plants were obtained from tissue and protoplast-derived calli through meristematic organogenesis. The results have been discussed in relation to somatic interspecific hybridization within the genus Medicago.
Potato II medium was tested against B5 medium for their ability to induce pollen callus in anther culture of a hexaploid triticale Beagle and two F1 hybrids between Beagle and wheat. Anthers of all three tested genotypes produced much more calli and two of them produced much more green plants on Potato II medium than those on B5 medium. The highest yields, i.e. 48.9 calli and 11.5 green plants from 100 cultured anthers, were achieved in F1 of Beagle × Kedong No. 58.
Chloroplast genomes of sugar beet (Beta vulgaris) and its five related species have been characterized by restriction enzyme analysis. B. vulgaris and B. maritima chloroplast (ct) DNAs share almost identical restriction patterns irrespective of restriction enzymes used, indicating a close relationship between the ctDNAs of these two species assigned to the section Vulgares. In contrast, ctDNAs from the other four species (B. trigyna, B. lomatogona, B. patellaris and B. procumbens) show clear differences. Thus, closely related species display more similar ctDNA fragment patterns than do distantly related ones. It was also demonstrated that variations in ctDNAs have occurred in the fragment carrying a putative gene (rps19) for ribosomal protein CS-19. These findings indicate that the taxonomic classification shows a parallel relation with the ctDNA diversity in Beta.
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