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Preparative biochemistry最新文献

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Bead centrifuge tube inserts--a semi-quantitative theory. 1984. 头离心管插入-半定量理论。1984.
Preparative biochemistry
Pub Date : 1993-02-01 DOI: 10.1080/10826069308544543
A Polson, K J van der Merwe
A semi-quantitative theory is proposed for the sedimentation of particles in centrifuge tubes filled with polymer beads acting as sedimentation aids. The theory concerns the attainment of approximate sedimentation-diffusion equilibrium in minimum time of centrifugation. It is shown empirically that the time to reach this state is approximately proportional to the diameter of the polymer beads as predicted by the theory.
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引用次数: 0
Isolation of viruses by electro-extraction of infected plants. 1977. 用电萃取法分离感染植物的病毒。1977.
Preparative biochemistry
Pub Date : 1993-02-01 DOI: 10.1080/10826069308544536
A Polson
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引用次数: 0
A rapid method for the purification of the MEF1 strain of poliomyelitis virus. 1954. 一种快速纯化脊髓灰质炎病毒MEF1株的方法。1954.
Preparative biochemistry
Pub Date : 1993-02-01 DOI: 10.1080/10826069308544545
A Polson, G Selzer
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引用次数: 0
Studies on poliomyelitis virus--concentration and purification of the virus. 1957. 脊髓灰质炎病毒的研究——病毒的浓缩和纯化。1957.
Preparative biochemistry
Pub Date : 1993-02-01 DOI: 10.1080/10826069308544548
A Polson, J W Hampton
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引用次数: 0
Special issue on virus separation and purification methods. 病毒分离和纯化方法特刊。
Preparative biochemistry
Pub Date : 1993-02-01
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引用次数: 0
An insert for the reorienting gradient rotor for virus extraction from infected plants. 1979. 用于从受感染植物中提取病毒的重定向梯度转子的插入件。1979.
Preparative biochemistry
Pub Date : 1993-02-01 DOI: 10.1080/10826069308544538
A Polson
A device made of nylon and which is inserted in the bowl of the reorienting gradient rotor is described. This insert acts as a container for holding infected plant tissues for the purpose of separating virus by centrifugal force from intact and fresh plants, from frozen and thawed plants and from plant tissue disintegrated by mechanical means. The extracted fluid represents 80 to 90% of that present in the untreated plant tissue. Electron micrographs taken of concentrates of the viruses prepared by the centrifugation extraction procedure indicate that extraneous materials are reduced to a low level.
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引用次数: 0
Investigations of poliomyelitis antigens. 1958. 脊髓灰质炎抗原的调查。1958.
Preparative biochemistry
Pub Date : 1993-02-01 DOI: 10.1080/10826069308544549
A Polson, A Ehrenberg, R Cramer
The complex nature of the antigens in suckling mouse-adapted MEFi virus was indicated by Selzer and Poison1, who found that the infectivity was associated with two components of approximate sedimentation coefficients 100 and 173 S respectively. In addition to these components a “soluble antigen” was found which had a sedimentation coefficient of about 22 S. These results, which were obtained by using a Spinco preparative centrifuge (Poison and Linder2), have now been confirmed in the Spinco analytical ultracentrifuge of the Nobel Institute, Stockholm, and other types of poliomyelitis virus have been studied in both instruments.
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引用次数: 0
The sedimenting-droplet procedure for measuring fluid densities. 1980. 测量流体密度的沉淀液滴法。1980.
Preparative biochemistry
Pub Date : 1993-02-01 DOI: 10.1080/10826069308544540
A Polson, H Alk, K Achtleitner
An apparatus is described which is used for the determination of velocities of falling droplets of small volumes (5/xl) through hydrophobic mixtures. From calibration curves with sodium chloride solutions of different molarities and specific gravities, the densities of the fluid in the droplets were obtained by interpolation. The density of the solutions of proteins may be determined and used in the calculation of partial specific volumes. The hydrophobic fluid used was a mixture of Dow Corning 200, kerosine to reduce the viscosity, and 1.2-dichlorobenzine to increase the specific gravity to approximately that of water.
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引用次数: 0
Determination of the number of Fab1 pieces which react with high molecular weight antigens. 1982. 测定与高分子量抗原发生反应的Fab1片段数目。1982.
Preparative biochemistry
Pub Date : 1993-02-01 DOI: 10.1080/10826069308544541
A Polson, K J van der Merwe
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引用次数: 0
Purification of viruses by centrifugation in gradients of inert substances. 用惰性物质梯度离心纯化病毒。
Preparative biochemistry
Pub Date : 1993-02-01 DOI: 10.1080/10826069308544544
A Polson

By using a reorienting gradient centrifuge rotor cut from a block of Nylon and fitted with eight septae, it was possible to separate the components of the haemolymph of the mollusc Turbo sarmaticus into three fractions in a sucrose gradient held in the bowl of the rotor. The fractions were (108 and 98)S, 44S and 16-22S. The success of the experiment was due to the large differences in the sedimentation coefficients of the components. When the rotor was applied to the natural mixture of the five viruses of the caterpillars of Nudaurelia cytheria only the main component could be isolated in a pure state. The viruses were separated by isopycnic centrifugation in "self formed" caesium chloride gradients, using a Beckman Model E analytical centrifuge in which a separation cell fitted with a centerpiece with two perforated partitions was used.

通过使用由尼龙块切割而成并装有8个隔膜的重定向梯度离心机转子，可以将Turbo sarmaticus软体动物的血淋巴成分在转子碗中的蔗糖梯度中分离成三部分。分数分别为(108和98)S、44S和16-22S。实验的成功是由于各组分的沉降系数差异很大。将转子应用于五种绿绒绒虫幼虫病毒的自然混合物中，只有主成分能在纯净状态下分离出来。采用贝克曼E型分析离心机，在“自形成”的氯化铯梯度中进行等异离心分离病毒，其中使用了一个配有两个穿孔隔板的中心部件的分离细胞。
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引用次数: 0
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