{"title":"Loss-of-function mutations in athletes","authors":"","doi":"10.18699/sbb-2022-219","DOIUrl":"https://doi.org/10.18699/sbb-2022-219","url":null,"abstract":"","PeriodicalId":204247,"journal":{"name":"The Thirteenth International Multiconference","volume":"44 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132185534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Exploration of natural flavonoids as novel Topoisomerase II inhibitors using DFT, molecular docking, and molecular dynamics","authors":"","doi":"10.18699/sbb-2022-208","DOIUrl":"https://doi.org/10.18699/sbb-2022-208","url":null,"abstract":"","PeriodicalId":204247,"journal":{"name":"The Thirteenth International Multiconference","volume":"40 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132692316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Modern models of brain diseases for translational studies","authors":"","doi":"10.18699/sbb-2022-430","DOIUrl":"https://doi.org/10.18699/sbb-2022-430","url":null,"abstract":"","PeriodicalId":204247,"journal":{"name":"The Thirteenth International Multiconference","volume":"55 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133159561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"804 BGRS/SB-2022 Artificial intelligence (AI) of 3D MRI images for neurooncology","authors":"","doi":"10.18699/sbb-2022-466","DOIUrl":"https://doi.org/10.18699/sbb-2022-466","url":null,"abstract":"","PeriodicalId":204247,"journal":{"name":"The Thirteenth International Multiconference","volume":"36 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133212078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The real function of the gene TERMINAL FLOWER 1","authors":"","doi":"10.18699/sbb-2022-370","DOIUrl":"https://doi.org/10.18699/sbb-2022-370","url":null,"abstract":"","PeriodicalId":204247,"journal":{"name":"The Thirteenth International Multiconference","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127867074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Structure and dynamics of the SARS-CoV-2 envelope protein","authors":"","doi":"10.18699/sbb-2022-167","DOIUrl":"https://doi.org/10.18699/sbb-2022-167","url":null,"abstract":"","PeriodicalId":204247,"journal":{"name":"The Thirteenth International Multiconference","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127870530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Reconstruction and analysis of the gene network of hepatocellular carcinoma under mechanical stress of the cell","authors":"","doi":"10.18699/sbb-2022-128","DOIUrl":"https://doi.org/10.18699/sbb-2022-128","url":null,"abstract":"","PeriodicalId":204247,"journal":{"name":"The Thirteenth International Multiconference","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131755633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Michurina, S. Miroshnichenko, I. Ishchenko, A. E. Serykh, L. N. Rachkovskaya
More and more data is accumulating about the close connection between the pineal gland and the immune system. Prolonged continuous lighting contributes to the development of light-induced functional pinealectomy, as a result of which melatonin (MT) synthesis in the pineal gland is reduced/blocked. It was found that the character of the 24-hour rhythms of the number of B- and T- cells, T-helper cells and T-suppressors/killers, the expression of CD45, CD5, CD3 and CD4 surface molecules on rat lymphocytes depends on the light regime and changes when animals are kept in conditions of constant light or constant darkness (Pelegri C. et al., 2003). Situations with functional pinealectomy or MT production inhibition lead to immunosuppression, to a decrease in adaptive cellular and humoral immune response. The use of this hormone helps to restore the immune system functions. However, the peculiarities of MT effect on the thymus cellular composition of animals under the conditions of functional pinealectomy remain not fully investigated. Thymus samples were taken to study its cellular composition using a flow cytofluorimeter. A cell suspension was prepared from the thymus by soft crushing in a glass homogenizer. Then fixation was carried out in ice 70% ethanol. Immunophenotyping of thymic CD3 hi and CD3 low T-lymphocyte subpopulations was performed by staining with CD3-APC antibodies (BioLegend Inc., USA). 3 µl of monoclonal antibody in the presence of 1% albumin was added to 50 µl of each sample. The samples were incubated at room temperature in the dark for 30 min and then washed twice with PBS. The analysis of obtained thymocytes was carried out using a CytoFlexS-100 flow cytofluorimeter (Beckman Coulter, Inc, USA). The CD3 low /CD3 hi ratio was calculated. Statistical processing was performed using the software package "Statistica12". Median values, values of the first and third quartiles, and the arithmetic mean were determined. The reliability of the differences between compared values was determined using Mann-Whitney test. The differences were considered statistically significant: at p<0.05 - *, at p<0.01 - **.
越来越多的数据表明，松果体与免疫系统之间有着密切的联系。长时间的连续照明有助于光诱导的功能性松果体切除术的发展，其结果是松果体中褪黑激素(MT)的合成减少/受阻。研究发现，B细胞和T细胞、T辅助细胞和T抑制细胞/杀伤细胞数量的24小时节律特征，以及CD45、CD5、CD3和CD4表面分子在大鼠淋巴细胞上的表达取决于光照制度，并在动物处于恒定光照或恒定黑暗条件下发生变化(Pelegri C. et al.， 2003)。功能性松果体切除术或MT产生抑制导致免疫抑制，降低适应性细胞和体液免疫反应。使用这种激素有助于恢复免疫系统功能。然而，在功能性松果体切除术条件下，MT对动物胸腺细胞组成影响的特殊性尚未得到充分研究。采用流式细胞荧光仪研究胸腺样本的细胞组成。以胸腺为原料，在玻璃均质机中进行软粉碎制备细胞悬液。然后用70%乙醇冰固定。用CD3- apc抗体(BioLegend Inc.， USA)染色对胸腺CD3高和CD3低t淋巴细胞亚群进行免疫分型。在每个样品的50µl中加入1%白蛋白存在的3µl单克隆抗体。样品在室温黑暗中孵育30分钟，然后用PBS洗涤两次。使用cytoflex -100流式细胞荧光仪(Beckman Coulter, Inc, USA)对获得的胸腺细胞进行分析。计算CD3低/CD3高比值。采用“Statistica12”软件包进行统计处理。确定中位数、第一和第三四分位数的值以及算术平均值。比较值间差异的信度采用Mann-Whitney检验。认为差异有统计学意义:p<0.05 - *， p<0.01 - **。
{"title":"Melatonin effect on the thymus cell composition in mice with functional pinealectomy","authors":"S. Michurina, S. Miroshnichenko, I. Ishchenko, A. E. Serykh, L. N. Rachkovskaya","doi":"10.18699/sbb-2022-469","DOIUrl":"https://doi.org/10.18699/sbb-2022-469","url":null,"abstract":"More and more data is accumulating about the close connection between the pineal gland and the immune system. Prolonged continuous lighting contributes to the development of light-induced functional pinealectomy, as a result of which melatonin (MT) synthesis in the pineal gland is reduced/blocked. It was found that the character of the 24-hour rhythms of the number of B- and T- cells, T-helper cells and T-suppressors/killers, the expression of CD45, CD5, CD3 and CD4 surface molecules on rat lymphocytes depends on the light regime and changes when animals are kept in conditions of constant light or constant darkness (Pelegri C. et al., 2003). Situations with functional pinealectomy or MT production inhibition lead to immunosuppression, to a decrease in adaptive cellular and humoral immune response. The use of this hormone helps to restore the immune system functions. However, the peculiarities of MT effect on the thymus cellular composition of animals under the conditions of functional pinealectomy remain not fully investigated. Thymus samples were taken to study its cellular composition using a flow cytofluorimeter. A cell suspension was prepared from the thymus by soft crushing in a glass homogenizer. Then fixation was carried out in ice 70% ethanol. Immunophenotyping of thymic CD3 hi and CD3 low T-lymphocyte subpopulations was performed by staining with CD3-APC antibodies (BioLegend Inc., USA). 3 µl of monoclonal antibody in the presence of 1% albumin was added to 50 µl of each sample. The samples were incubated at room temperature in the dark for 30 min and then washed twice with PBS. The analysis of obtained thymocytes was carried out using a CytoFlexS-100 flow cytofluorimeter (Beckman Coulter, Inc, USA). The CD3 low /CD3 hi ratio was calculated. Statistical processing was performed using the software package \"Statistica12\". Median values, values of the first and third quartiles, and the arithmetic mean were determined. The reliability of the differences between compared values was determined using Mann-Whitney test. The differences were considered statistically significant: at p<0.05 - *, at p<0.01 - **.","PeriodicalId":204247,"journal":{"name":"The Thirteenth International Multiconference","volume":"118 3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127423656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Correlation of resting-state EEG indicators with personal and genetic characteristics of subjects living in different socio-climatic conditions","authors":"","doi":"10.18699/sbb-2022-553","DOIUrl":"https://doi.org/10.18699/sbb-2022-553","url":null,"abstract":"","PeriodicalId":204247,"journal":{"name":"The Thirteenth International Multiconference","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115451656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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