Pub Date : 2023-09-18DOI: 10.1007/s40883-023-00317-x
Shiv Shah, Debolina Ghosh, Takayoshi Otsuka, Cato T. Laurencin
{"title":"Classes of Stem Cells: From Biology to Engineering","authors":"Shiv Shah, Debolina Ghosh, Takayoshi Otsuka, Cato T. Laurencin","doi":"10.1007/s40883-023-00317-x","DOIUrl":"https://doi.org/10.1007/s40883-023-00317-x","url":null,"abstract":"","PeriodicalId":20936,"journal":{"name":"Regenerative Engineering and Translational Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135151239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
<p><strong>Purpose: </strong>This study demonstrated <i>in vivo</i> delivery of a decellularized, injectable peripheral nerve (iPN) hydrogel and explored options for using iPN in combination with regenerative biomolecular therapies like stem cell secretome.</p><p><strong>Methods: </strong>Rat-derived iPN hydrogel solutions were combined with a dextran-dye before subcutaneous injection into adult Sprague Dawley rats. After injection, an <i>in vivo</i> imaging system (IVIS) was used to visualize hydrogels and quantify dextran-dye release over time. Poly(lactic-co-glycolic) acid (PLGA) was used to encapsulate the dextran-dye to prolong molecular release from the hydrogel scaffolds. Lastly, we investigated use of adipose-derived stem cell (ASC) secretome as a potential future combination strategy with iPN. ASC secretome was assessed for growth factor levels in response to media stimulation and was encapsulated in PLGA to determine loading efficiency.</p><p><strong>Results: </strong>Gelation of iPN hydrogels was successful upon subcutaneous injection. When combined with iPN, a 10 kDa dextran-dye was reduced to 54% its initial signal at 24 hours, while PLGA-encapsulated dextran-dye in iPN was only reduced to 78% by 24 hours. Modified media stimulation resulted in changes in ASC phenotype and dramatic upregulation of VEGF secretion. The PLGA encapsulation protocol was adapted for use with temperature sensitive biomolecules, however, considerations must be made with loading efficiency for cell secretome as the maximum efficiency was 28%.</p><p><strong>Conclusion: </strong>The results of this study demonstrated successful injection and subsequent gelation of our iPN hydrogel formulation <i>in vivo</i>. Biomolecular payloads can be encapsulated in PLGA to help prolong their release from the soft iPN hydrogels in future combination therapies.</p><p><strong>Lay summary: </strong>We developed an injectable decellularized tissue scaffold from rat peripheral nerve tissue (called iPN), a potential minimally invasive therapeutic meant to fill lesion spaces after injury. This study was the first demonstration of iPN delivery to a living animal. The iPN solution was injected subcutaneously in a rat and properly formed a gelled material upon entering the body. Our results showed that encapsulating biomolecules in an FDA-approved polymer (PLGA) slowed the release of biomolecules from the iPN, which could allow therapeutics more time around the scaffold to help repair native tissue. Lastly, we investigated one potential avenue for combining iPN with other regenerative cues obtained from adipose-derived stem cells.</p><p><strong>Description of future works: </strong>Future work must focus on optimal loading conditions and release profiles from the iPN hydrogels. Next steps will be applying iPN in various combination therapies for spinal cord injury. We will focus efforts on developing a pro-regenerative secretome that directly promotes neurite extension and neural cell
{"title":"Injectable neural hydrogel as <i>in vivo</i> therapeutic delivery vehicle.","authors":"Nora Hlavac, Deanna Bousalis, Emily Pallack, Yuan Li, Eleana Manousiouthakis, Raffae Ahmad, Christine E Schmidt","doi":"10.1007/s40883-022-00292-9","DOIUrl":"10.1007/s40883-022-00292-9","url":null,"abstract":"<p><strong>Purpose: </strong>This study demonstrated <i>in vivo</i> delivery of a decellularized, injectable peripheral nerve (iPN) hydrogel and explored options for using iPN in combination with regenerative biomolecular therapies like stem cell secretome.</p><p><strong>Methods: </strong>Rat-derived iPN hydrogel solutions were combined with a dextran-dye before subcutaneous injection into adult Sprague Dawley rats. After injection, an <i>in vivo</i> imaging system (IVIS) was used to visualize hydrogels and quantify dextran-dye release over time. Poly(lactic-co-glycolic) acid (PLGA) was used to encapsulate the dextran-dye to prolong molecular release from the hydrogel scaffolds. Lastly, we investigated use of adipose-derived stem cell (ASC) secretome as a potential future combination strategy with iPN. ASC secretome was assessed for growth factor levels in response to media stimulation and was encapsulated in PLGA to determine loading efficiency.</p><p><strong>Results: </strong>Gelation of iPN hydrogels was successful upon subcutaneous injection. When combined with iPN, a 10 kDa dextran-dye was reduced to 54% its initial signal at 24 hours, while PLGA-encapsulated dextran-dye in iPN was only reduced to 78% by 24 hours. Modified media stimulation resulted in changes in ASC phenotype and dramatic upregulation of VEGF secretion. The PLGA encapsulation protocol was adapted for use with temperature sensitive biomolecules, however, considerations must be made with loading efficiency for cell secretome as the maximum efficiency was 28%.</p><p><strong>Conclusion: </strong>The results of this study demonstrated successful injection and subsequent gelation of our iPN hydrogel formulation <i>in vivo</i>. Biomolecular payloads can be encapsulated in PLGA to help prolong their release from the soft iPN hydrogels in future combination therapies.</p><p><strong>Lay summary: </strong>We developed an injectable decellularized tissue scaffold from rat peripheral nerve tissue (called iPN), a potential minimally invasive therapeutic meant to fill lesion spaces after injury. This study was the first demonstration of iPN delivery to a living animal. The iPN solution was injected subcutaneously in a rat and properly formed a gelled material upon entering the body. Our results showed that encapsulating biomolecules in an FDA-approved polymer (PLGA) slowed the release of biomolecules from the iPN, which could allow therapeutics more time around the scaffold to help repair native tissue. Lastly, we investigated one potential avenue for combining iPN with other regenerative cues obtained from adipose-derived stem cells.</p><p><strong>Description of future works: </strong>Future work must focus on optimal loading conditions and release profiles from the iPN hydrogels. Next steps will be applying iPN in various combination therapies for spinal cord injury. We will focus efforts on developing a pro-regenerative secretome that directly promotes neurite extension and neural cell","PeriodicalId":20936,"journal":{"name":"Regenerative Engineering and Translational Medicine","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10683944/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82719554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-09DOI: 10.1007/s40883-023-00316-y
E. Reza, Hossein Azizi
{"title":"Correlation Between Alkaline Phosphatase Expression and Sox2, Oct4, and Nanog Genes in Spermatogonial and ES-Like Cells","authors":"E. Reza, Hossein Azizi","doi":"10.1007/s40883-023-00316-y","DOIUrl":"https://doi.org/10.1007/s40883-023-00316-y","url":null,"abstract":"","PeriodicalId":20936,"journal":{"name":"Regenerative Engineering and Translational Medicine","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85040486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-09DOI: 10.1007/s40883-023-00314-0
Jayavardhini Bhoopathy, Lokesh Prabakaran, Weslen Vedakumari Sathyaraj, R. Karthikeyan, R. Senthil
{"title":"A Comprehensive Review on Natural Therapeutics for Wound Treatment","authors":"Jayavardhini Bhoopathy, Lokesh Prabakaran, Weslen Vedakumari Sathyaraj, R. Karthikeyan, R. Senthil","doi":"10.1007/s40883-023-00314-0","DOIUrl":"https://doi.org/10.1007/s40883-023-00314-0","url":null,"abstract":"","PeriodicalId":20936,"journal":{"name":"Regenerative Engineering and Translational Medicine","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82130181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-02DOI: 10.1007/s40883-023-00315-z
J. Husch, Laura Coquelin, N. Chevallier, Natasja W M van Dijk, S. Leeuwenburgh, J. J. van den Beucken
{"title":"Human Macrophage- and Osteoclast-Based Constructs Do Not Induce Ectopic Bone Formation","authors":"J. Husch, Laura Coquelin, N. Chevallier, Natasja W M van Dijk, S. Leeuwenburgh, J. J. van den Beucken","doi":"10.1007/s40883-023-00315-z","DOIUrl":"https://doi.org/10.1007/s40883-023-00315-z","url":null,"abstract":"","PeriodicalId":20936,"journal":{"name":"Regenerative Engineering and Translational Medicine","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87786982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-18DOI: 10.1007/s40883-023-00312-2
F. Assanah, Hanna Anderson, K. Grassie, L. Nair, Y. Khan
{"title":"Ultrasound-Derived Mechanical Stimulation of Alginate Hydrogels for Bone Repair: an In Vitro Study","authors":"F. Assanah, Hanna Anderson, K. Grassie, L. Nair, Y. Khan","doi":"10.1007/s40883-023-00312-2","DOIUrl":"https://doi.org/10.1007/s40883-023-00312-2","url":null,"abstract":"","PeriodicalId":20936,"journal":{"name":"Regenerative Engineering and Translational Medicine","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77117111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-17DOI: 10.1007/s40883-023-00308-y
Nahla Imbarak, H. Korayem, N. Soliman, S. Hosny
{"title":"Effect of Bone Marrow Mesenchymal Stem Cells on a Short-Term Induced Diabetic Retinopathy in Adult Female Albino Rats","authors":"Nahla Imbarak, H. Korayem, N. Soliman, S. Hosny","doi":"10.1007/s40883-023-00308-y","DOIUrl":"https://doi.org/10.1007/s40883-023-00308-y","url":null,"abstract":"","PeriodicalId":20936,"journal":{"name":"Regenerative Engineering and Translational Medicine","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76004082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-10DOI: 10.1007/s40883-022-00275-w
S. Almasi-turk, H. G. Hamidabadi, P. Farzadinia, A. Movahed, H. Salimipour, R. Nemati, A. Roozbehi, M. N. Bojnordi, S. Darabi, S. Mojaverrostami
{"title":"Human Umbilical Cord–derived Mesenchymal Stem Cells into Oligodendrocyte-like Cells using Triiodothyronine as an Inducer: a Rapid and Efficient Protocol","authors":"S. Almasi-turk, H. G. Hamidabadi, P. Farzadinia, A. Movahed, H. Salimipour, R. Nemati, A. Roozbehi, M. N. Bojnordi, S. Darabi, S. Mojaverrostami","doi":"10.1007/s40883-022-00275-w","DOIUrl":"https://doi.org/10.1007/s40883-022-00275-w","url":null,"abstract":"","PeriodicalId":20936,"journal":{"name":"Regenerative Engineering and Translational Medicine","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74866343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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