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Evaluation of the Superabsorbent Effect on the Yield and Some Growth Factors of Pisum sativum L. under Drought Stress Conditions. 干旱胁迫条件下超级吸水剂对豌豆产量和某些生长因子影响的评估
Q1 Agricultural and Biological Sciences Pub Date : 2019-01-01 DOI: 10.2174/2212798410666180924142716
Farahnaz A Norodinvand, Davoud K Dehkordi, Aslan Egdernezhad

Background: Deficit irrigation is an optimum technique for producing products under drought stress conditions. The superabsorbent hydrogel is a hydrophilic polymer with cross-linked 3-D hydrophilic nets that are able to take up and keep noteworthy values of water and aquatic liquids. Recently published patents have provided significant information about the superabsorbent application in agriculture.

Objective: The aim of this study was to investigate the superabsorbent effect on the yield and some of the growth factors of Pisum sativum L. under drought stress conditions.

Methods: The experimental factors included: irrigation treatments at two levels of 100% and 75% water requirement by the plant. The second factor included the levels of superabsorbent application which included three levels of control treatment, potting soil with a weight percentage of 0.5 and 1. The third factor was the location of the superabsorbent application.

Results: According to the results, the largest root length value corresponded to I2S1U treatment plan, the largest root dry weight value corresponded to I1S2U treatment plan, the largest plant dry weight corresponded to I2S1U treatment plan, the largest grain dry weight corresponded to I2S1U treatment plan, the largest number of grains per pod corresponded to I2S2U treatment plan and the largest number of pods per plant corresponded to I2S1U treatment plan which were significant at 5% level.

Conclusion: It was concluded that the presence of the superabsorbent at the lower end of the pot was effective in providing water and nutrients for the plant root.

背景:缺水灌溉是在干旱胁迫条件下生产产品的最佳技术。超吸水性水凝胶是一种具有交联三维亲水网的亲水性聚合物,能够吸收和保持值得注意的水和水生液体。最近公布的专利提供了有关超吸水剂在农业中应用的重要信息:本研究旨在探讨在干旱胁迫条件下超吸水剂对豌豆产量和一些生长因子的影响:实验因素包括:植物需水量为 100% 和 75% 的两种灌溉处理。第二个因素包括超吸收剂的施用水平,包括对照处理、重量百分比为 0.5 和 1 的盆栽土壤三个水平,第三个因素是超吸收剂的施用位置:结果:I2S1U 处理方案对应的根长值最大,I1S2U 处理方案对应的根干重值最大,I2S1U 处理方案对应的植株干重最大,I2S1U 处理方案对应的谷粒干重最大,I2S2U 处理方案对应的每荚谷粒数最多,I2S1U 处理方案对应的每株荚果数最多,均在 5%的水平上显著:结论:在花盆下端使用超级吸水剂能有效地为植物根部提供水分和养分。
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引用次数: 3
Trends on the Rapid Expansion of Supercritical Solutions Process Applied to Food and Non-food Industries. 超临界溶液工艺在食品和非食品工业中的快速发展趋势。
Q1 Agricultural and Biological Sciences Pub Date : 2019-01-01 DOI: 10.2174/2212798410666180925160459
Maria T M S Gomes, Ádina L Santana, Diego T Santos, Maria A A Meireles

Background: The supercritical fluids applied to particle engineering over the last years have received growing interest from the food and non-food industries, in terms of processing, packaging, and preservation of several products. The rapid expansion of supercritical solutions (RESS) process has been recently reported as an efficient technique for the production of free-solvent particles with controlled morphology and size distribution.

Objective: In this review, we report technological aspects of the application of the RESS process applied to the food and non-food industry, considering recent data and patent survey registered in literature.

Methods: The effect of process parameters cosolvent addition, temperature, pressure, nozzle size among others, during RESS on the size, structure and morphology of the resulted particles, and the main differences about recent patented RESS processes are reviewed.

Results: Most of the experimental works intend to optimize their processes through investigation of process parameters.

Conclusion: RESS is a feasible alternative for the production of particles with a high yield of bioactive constituents of interest to the food industry. On the other hand, patents developed using this type of process for food products are very scarce, less attention being given to the potential of this technique to develop particles from plant extracts with bioactive substances.

背景:近年来,超临界流体在颗粒工程中的应用受到了食品和非食品行业越来越多的关注,主要用于几种产品的加工、包装和保存。超临界溶液快速膨胀(RESS)工艺最近被报道为一种有效的技术,用于生产具有控制形态和尺寸分布的自由溶剂颗粒。目的:在这篇综述中,我们考虑到最近的数据和文献中注册的专利调查,报告了在食品和非食品工业中应用RESS工艺的技术方面的应用。方法:综述了制备过程中共溶剂添加量、温度、压力、喷嘴尺寸等工艺参数对制备的颗粒尺寸、结构和形貌的影响,以及近年来制备工艺的主要差异。结果:大多数实验都是通过对工艺参数的考察来优化工艺。结论:RESS是一种可行的生产颗粒的替代方法,具有高产量的食品工业感兴趣的生物活性成分。另一方面,利用这类工艺开发的食品专利非常少,很少有人注意到这种技术从具有生物活性物质的植物提取物中开发颗粒的潜力。
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引用次数: 6
Effect of Trichoderma reesei Degraded Date Pits Supplementation on Growth Performance, Immunoglobulin Levels and Intestinal Barrier Functions of Broiler Chickens. 添加降解里氏木霉枣核对肉仔鸡生长性能、免疫球蛋白水平和肠道屏障功能的影响
Q1 Agricultural and Biological Sciences Pub Date : 2019-01-01 DOI: 10.2174/2212798410666190716163009
Salem R. Alyileili, K. El-Tarabily, Wissam H Ibrahim, M. Sulaiman, A. Hussein
BACKGROUND Date palm (Phoenix dactylifera L.) is a dominant fruit crop in most of the Arabian countries. Date pits, as a major byproduct which remained after consumption of date flesh proved to be valuable source of energy. OBJECTIVES The impact of degraded date pits (DDP) on growth performance, intestinal bacterial population and expression profiles of intestinal genes in broilers were determined. METHODS Solid state degradation system (SSD) was used for the preparation of DDP using Trichoderma reesei. One-day-old Brazilian broiler chicks "Cobb 500" were randomly divided into six treatments with six replicates, which consisted of a normal diet containing only corn-soy (control), diet containing corn-soy + (20%, 50g/100Kg oxytetracycline), diet containing corn soy + 10% (DDP), diet containing corn-soy + 0.2% mannan oligosaccharides (MOS), diet containing corn-soy + 0.1% mannose and diet containing corn-soy + 0.2% mannose. RESULTS There was no significant differences in body weight, feed intake and feed conversion ratio (FCR) in broilers among the treatments. Bacterial count was significantly decreased in 10% DDP diet fed broilers, 0.2% MOS and antibiotic diet fed broilers. Immunoglobulin levels in serum and intestinal contents and expression pattern of genes in jejunum were upregulated in 10% DDP and 0.2% MOS diet fed broilers. CONCLUSION DDP can be used as an energy source for replacing part of corn, mannan oligosaccharide and also recommended as a potential alternative to antimicrobials in broilers diet.
背景枣棕榈(Phoenix dactylifera L.)是大多数阿拉伯国家的主要水果作物。枣核作为枣果肉食用后的主要副产品,是一种宝贵的能源。目的研究降解枣核(DDP)对肉鸡生长性能、肠道菌群及肠道基因表达谱的影响。方法以里氏木霉为原料,采用固态降解法制备DDP。试验选用1日龄巴西肉鸡“Cobb 500”,随机分为6个处理,每6个重复,分别为:正常饲粮中只添加玉米-大豆(对照)、玉米-大豆+ (20%,50g/100Kg土霉素)、玉米-大豆+ 10% (DDP)、玉米-大豆+ 0.2%甘露聚糖(MOS)、玉米-大豆+ 0.1%甘露糖和玉米-大豆+ 0.2%甘露糖。结果各处理肉鸡体重、采食量和饲料系数均无显著差异。10% DDP饲粮、0.2% MOS饲粮和抗生素饲粮显著降低了肉鸡细菌数量。10% DDP和0.2% MOS饲粮上调了肉鸡血清、肠道免疫球蛋白水平和空肠基因表达模式。结论ddp可作为肉仔鸡饲粮中部分玉米、甘露寡糖的能量来源,也可作为抗微生物药物的潜在替代品。
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引用次数: 7
Evaluation of Nutritional Supplementation with Palm Oil in High-Gravity Beer Production. 棕榈油在高重力啤酒生产中的营养补充评价。
Q1 Agricultural and Biological Sciences Pub Date : 2019-01-01 DOI: 10.2174/2212798410666180723154633
Caroline C A Magalhães, Julia A Romão, Geiza S Araújo, Diego T Santos, Giovani B M De Carvalho

Background: The use of nutritional supplementation of the brewer's wort can be an interesting option to increase cell viability and yeast fermentability.

Objective: This study aims to evaluate the effects of the variables wort concentration and nutritional supplementation with palm oil in the production of beer in high-density wort.

Methods: The process effects were evaluated through the central composite rotational design of type 22 associated with the Response Surface Methodology (RSM). The fermentations were carried out using the commercial Saccharomyces cerevisiae yeast, lager type, at 15°C.

Results: The mathematical models and RSM obtained were an efficienct strategy to determine the optimum fermentation point for the ethanol volumetric productivity (wort concentration of 20.90 °P and palm oil content of 0.19 % v/v) and for the apparent degree of fermentation (wort concentration of 16.90 °P and palm oil content of 0.22% v/v). There was a good correlation between the experimental values observed and predicted by the model, indicating that the fit of the model was satisfactory and it can be inferred that the increase of the wort concentration and the nutritional supplementation with the palm oil reached an ethanol volumetric productivity of 0.55 g/L.h and an apparent degree of fermentation of 50.20 %.

Conclusion: Therefore, it can be concluded that our study demonstrates that nutritional supplementation with palm oil is an alternative and promising option for the breweries to increase productivity. There are recent patents also suggesting the advantages of using alternative nutritional supplements in beverage production.

背景:使用啤酒麦芽汁的营养补充可以是一个有趣的选择,以增加细胞活力和酵母发酵能力。目的:研究以高密度麦汁为原料,考察麦汁浓度和棕榈油营养补充对啤酒生产的影响。方法:采用22型中心复合旋转设计,结合响应面法(RSM)评价工艺效果。在15°C的条件下,使用商业酿酒酵母(窖藏型)进行发酵。结果:所建立的数学模型和RSM是确定乙醇体积产率(麦汁浓度为20.90°P,棕榈油含量为0.19% v/v)和表观发酵度(麦汁浓度为16.90°P,棕榈油含量为0.22% v/v)最佳发酵点的有效策略。实验结果与模型预测值具有良好的相关性,表明模型拟合良好,可以推断,增加麦汁浓度和添加棕榈油的乙醇体积产率达到0.55 g/L.h,表观发酵度达到50.20%。结论:因此,我们的研究表明,棕榈油的营养补充是酿酒厂提高生产力的另一种有前途的选择。最近的一些专利也表明了在饮料生产中使用替代营养补充剂的好处。
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引用次数: 0
Strategies for Rot Control of Soybean Sprouts. 大豆芽腐烂病防治策略。
Q1 Agricultural and Biological Sciences Pub Date : 2019-01-01 DOI: 10.2174/2212798410666181116121957
Jae-Suk Choi

Soybean sprouts are nutrient-rich, contain plentiful proteins, vitamin C, and minerals and are packed in small numbers after production. As soybean sprouts were mass produced in a factory, the occurrence of rotting in soybean sprouts has become a serious problem. To overcome these problems, many efforts have been made to provide healthy soybean sprouts in Korea. This paper reviewed the physicochemical techniques used for supplying water with antibacterial properties and the natural antimicrobial materials developed for soybean sprout cultivation. On the basis of this review, 11 of the antimicrobial agents and/or techniques currently used originated from mineral, non-metal ions, and metal ions, 4 from antagonistic microorganisms, 7 from agents originating from animals, 31 from medicinal and herbal plants, and 11 from physicochemical agents and/or techniques. In addition, these agents and/or techniques showed potential not only for the inhibition of spoilage and rot of soybean sprouts but also for the extension of product shelf life, the enhancement of taste and aroma, the enhancement of nutrition and functional components, growth promotion, and/or the reduction of production costs. Continuous scientific innovations and improved processing technology will aid in further advancements and improvements in this area. Therefore, this study offers useful insights suggesting direction for future research and provides information on the different anti-rotting agents and/or techniques for soybean sprouts developed to date, also as discussed in various patents.

黄豆芽营养丰富,含有丰富的蛋白质、维生素 C 和矿物质,生产后少量包装。由于黄豆芽是在工厂中大量生产的,因此黄豆芽腐烂的现象已成为一个严重问题。为了克服这些问题,韩国为提供健康的黄豆芽做出了许多努力。本文综述了用于提供具有抗菌特性的水的物理化学技术,以及为黄豆芽栽培开发的天然抗菌材料。根据该综述,目前使用的抗菌剂和/或技术中有 11 种来自矿物、非金属离子和金属离子,4 种来自拮抗微生物,7 种来自动物,31 种来自药用和草本植物,11 种来自物理化学制剂和/或技术。此外,这些制剂和/或技术不仅在抑制大豆芽变质和腐烂方面具有潜力,而且在延长产品保质期、改善口感和香味、增加营养和功能成分、促进生长和/或降低生产成本方面也具有潜力。不断的科学创新和改进的加工技术将有助于该领域的进一步发展和改进。因此,本研究提供了有益的见解,为今后的研究指明了方向,并提供了有关迄今为止开发的不同大豆芽防腐剂和/或技术的信息,各种专利中也有论述。
{"title":"Strategies for Rot Control of Soybean Sprouts.","authors":"Jae-Suk Choi","doi":"10.2174/2212798410666181116121957","DOIUrl":"10.2174/2212798410666181116121957","url":null,"abstract":"<p><p>Soybean sprouts are nutrient-rich, contain plentiful proteins, vitamin C, and minerals and are packed in small numbers after production. As soybean sprouts were mass produced in a factory, the occurrence of rotting in soybean sprouts has become a serious problem. To overcome these problems, many efforts have been made to provide healthy soybean sprouts in Korea. This paper reviewed the physicochemical techniques used for supplying water with antibacterial properties and the natural antimicrobial materials developed for soybean sprout cultivation. On the basis of this review, 11 of the antimicrobial agents and/or techniques currently used originated from mineral, non-metal ions, and metal ions, 4 from antagonistic microorganisms, 7 from agents originating from animals, 31 from medicinal and herbal plants, and 11 from physicochemical agents and/or techniques. In addition, these agents and/or techniques showed potential not only for the inhibition of spoilage and rot of soybean sprouts but also for the extension of product shelf life, the enhancement of taste and aroma, the enhancement of nutrition and functional components, growth promotion, and/or the reduction of production costs. Continuous scientific innovations and improved processing technology will aid in further advancements and improvements in this area. Therefore, this study offers useful insights suggesting direction for future research and provides information on the different anti-rotting agents and/or techniques for soybean sprouts developed to date, also as discussed in various patents.</p>","PeriodicalId":21061,"journal":{"name":"Recent patents on food, nutrition & agriculture","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2174/2212798410666181116121957","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36737945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Impact of Electron Beam Irradiation on the Bioactive Principles of Seeds of Coastal Sand Dune Wild Legumes (Canavalia spp.). 电子束辐照对滨海沙丘野生豆科植物种子生物活性的影响
Q1 Agricultural and Biological Sciences Pub Date : 2019-01-01 DOI: 10.2174/2212798410666180709144200
Prabhavathi Supriya, Kandikere R Sridhar

Background: Recent patents reveal that vegetable ingredients have several applications in novel food formulations. Many so-called antinutritional components (e.g. tannins, saponins, lectins and protease inhibitors) have nutraceutical as well as pharmaceutical significance. Seeds of two wild legumes of the genus Canavalia inhabitants of the coastal sand dunes of Southwest India are known for a variety of bioactive principles (e.g. phenolics, tannins, canavanine, concanavalin and phytohemagglutinins).

Objective: This study evaluates the impact of Electron Beam (EB) irradiation on the bioactive components of seeds of two coastal sand dune wild legumes Canavalia cathartica and C. maritima.

Methods: The dry seeds of C. cathartica and C. maritima were EB irradiated with different doses (2.5, 5, 10 and 15 kGy) to follow changes in six bioactive principles (total phenolics, orthodihydric phenols, tannins, canavanine, trypsin inhibitors and phytohemagglutinins) in comparison to control seeds. One-way ANOVA was employed to follow the variation in bioactive components of seeds in control and different doses of irradiation.

Results: Seeds of both legumes were devoid of orthodihydric phenols and trypsin inhibitors. In C. cathartica, the total phenolics showed significant dose-dependent increase up to 5 kGy and decreased thereafter. Tannin content was not altered up to 10 kGy followed by significant decrease at 15 kGy. There was no significant change in canavanine content and the phytohemagglutinin activity against human erythrocytes was not altered. Seeds of C. maritima did not show significant changes in total phenolics as well as tannin contents. The content of canavanine showed significant dose-dependent increase up to 5 kGy followed by significant decrease. There was no variation in phytohemagglutinin activity against erythrocytes A, B and O, while against AB, the activity decreased at 2.5 kGy and further decrease was constant at higher doses.

Conclusion: The EB irradiation doses employed have selectively altered the bioactive principles of Canavalia seeds and such treatments may facilitate to maneuver desired medicinal, nutritional, functional and cooking properties. Besides selective changes in bioactive components the seeds have extended shelf life.

背景:最近的专利表明,蔬菜成分在新型食品配方中有几种应用。许多所谓的抗营养成分(如单宁、皂苷、凝集素和蛋白酶抑制剂)具有营养和药用意义。居住在印度西南部海岸沙丘上的两种野生豆科植物菜豆属的种子以其多种生物活性原理而闻名(例如酚类物质、单宁、菜豆氨酸、菜豆蛋白和植物血凝素)。目的:研究电子束辐照对两种滨海沙丘野生豆科植物菜豆(Canavalia cathartica)和海豆(C. martima)种子生物活性成分的影响。方法:采用不同剂量(2.5、5、10、15 kGy)的EB照射,观察两种植物的6种生物活性成分(总酚、正二氢酚、单宁、菜豆氨酸、胰蛋白酶抑制剂和植物血凝素)与对照种子的变化。采用单因素方差分析(One-way ANOVA)跟踪对照和不同辐照剂量下种子生物活性成分的变化。结果:两种豆科植物的种子均不含正二水酚和胰蛋白酶抑制剂。通风莲总酚含量在5kgy时呈显著的剂量依赖性增加,之后呈下降趋势。单宁含量在10 kGy以下没有变化,在15 kGy时显著降低。菜豆氨酸含量无明显变化,植物血凝素抗人红细胞活性未发生改变。海参种子总酚类物质和单宁含量变化不显著。5 kGy时,大麻碱含量呈显著剂量依赖性增加后又显著降低。植物血凝素对A、B和O红细胞的活性没有变化,而对AB红细胞的活性在2.5 kGy时下降,并且在更高剂量下继续下降。结论:EB辐照剂量可选择性地改变菜籽草种子的生物活性,使菜籽草的药用、营养、功能和烹饪特性发生改变。除了生物活性成分的选择性变化外,种子的保质期也延长了。
{"title":"Impact of Electron Beam Irradiation on the Bioactive Principles of Seeds of Coastal Sand Dune Wild Legumes (<i>Canavalia</i> spp.).","authors":"Prabhavathi Supriya,&nbsp;Kandikere R Sridhar","doi":"10.2174/2212798410666180709144200","DOIUrl":"https://doi.org/10.2174/2212798410666180709144200","url":null,"abstract":"<p><strong>Background: </strong>Recent patents reveal that vegetable ingredients have several applications in novel food formulations. Many so-called antinutritional components (e.g. tannins, saponins, lectins and protease inhibitors) have nutraceutical as well as pharmaceutical significance. Seeds of two wild legumes of the genus Canavalia inhabitants of the coastal sand dunes of Southwest India are known for a variety of bioactive principles (e.g. phenolics, tannins, canavanine, concanavalin and phytohemagglutinins).</p><p><strong>Objective: </strong>This study evaluates the impact of Electron Beam (EB) irradiation on the bioactive components of seeds of two coastal sand dune wild legumes Canavalia cathartica and C. maritima.</p><p><strong>Methods: </strong>The dry seeds of C. cathartica and C. maritima were EB irradiated with different doses (2.5, 5, 10 and 15 kGy) to follow changes in six bioactive principles (total phenolics, orthodihydric phenols, tannins, canavanine, trypsin inhibitors and phytohemagglutinins) in comparison to control seeds. One-way ANOVA was employed to follow the variation in bioactive components of seeds in control and different doses of irradiation.</p><p><strong>Results: </strong>Seeds of both legumes were devoid of orthodihydric phenols and trypsin inhibitors. In C. cathartica, the total phenolics showed significant dose-dependent increase up to 5 kGy and decreased thereafter. Tannin content was not altered up to 10 kGy followed by significant decrease at 15 kGy. There was no significant change in canavanine content and the phytohemagglutinin activity against human erythrocytes was not altered. Seeds of C. maritima did not show significant changes in total phenolics as well as tannin contents. The content of canavanine showed significant dose-dependent increase up to 5 kGy followed by significant decrease. There was no variation in phytohemagglutinin activity against erythrocytes A, B and O, while against AB, the activity decreased at 2.5 kGy and further decrease was constant at higher doses.</p><p><strong>Conclusion: </strong>The EB irradiation doses employed have selectively altered the bioactive principles of Canavalia seeds and such treatments may facilitate to maneuver desired medicinal, nutritional, functional and cooking properties. Besides selective changes in bioactive components the seeds have extended shelf life.</p>","PeriodicalId":21061,"journal":{"name":"Recent patents on food, nutrition & agriculture","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36293926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
Effects of PGPR (Pseudomonas sp.) and Ag-nanoparticles on enzymatic activity and physiology of cucumber. 假单胞菌和纳米银对黄瓜酶活性和生理的影响。
Q1 Agricultural and Biological Sciences Pub Date : 2019-01-01 DOI: 10.2174/2212798410666190716162340
S. Nawaz, A. Bano
The present investigation was aimed to evaluate the role of plant growth promoting rhizobacteria (PGPR) and Ag-nanoparticles on two varieties (American variety, Poinsett 76 and Desi variety, Sialkot selection) of cucumber plants. Cucumber seeds were inoculated prior to sowing with two strains of PGPR, Pseudomonas putida (KX574857) and Pseudomonas stutzeri (KX574858) at the rate of 106cells/ml. Ag-nanoparticles (5ppm) were sprayed on plant at early vegetative phase, 27 d after sowing. The proline, sugar, protein, phenolics, flavonoids, chlorophyll and carotenoids contents and the activities of phenylalanine ammonia-lyase (PAL), superoxide dismutase (SOD) and catalase (CAT) were determined from leaves of plants at early vegetative phase. Ag-nanoparticles enhanced the length of root but decreased the length of shoot and fresh weight of root and shoot as compared to control whereas, the leaf protein, proline, phenolics, flavonoids, chlorophyll b, total chlorophyll, sugar contents and the activity of phenylalanine ammonia-lyase (PAL) were increased significantly over control. Ag-nanoparticles also suppressed the effect of PGPR on root and shoot length but augmented the protein and phenolics contents of leaves of both the varieties. The combined treatment of Ag-nanoparticles and PGPR enhanced flavonoids content and the activities of defense related enzyme PAL and the antioxidant enzymes, superoxide dismutase (SOD) and catalase(CAT) in leaves of plants over control. Ag-nanoparticles was also stimulatory when applied alone. Pseudomonas putida may be used either alone or in combination with Ag-nanoparticles to enhance the antioxidant and defense enzyme activities to enable the plant to tolerate stresses in much better way.
本研究旨在评价促生根瘤菌(PGPR)和银纳米颗粒对黄瓜2个品种(美国品种Poinsett 76和Desi品种,Sialkot选择)的促生作用。播种前用恶臭假单胞菌(KX574857)和stutzeri假单胞菌(KX574858)两株PGPR菌株以106个细胞/ml的速率接种黄瓜种子。ag纳米颗粒(5ppm)在播种后27 d施用于植株营养早期。测定了植物营养早期叶片的脯氨酸、糖、蛋白质、酚类物质、黄酮类、叶绿素和类胡萝卜素含量以及苯丙氨酸解氨酶(PAL)、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性。与对照相比,银纳米颗粒增加了根的长度,降低了根和茎的长度和鲜重,而叶片蛋白质、脯氨酸、酚类物质、黄酮类、叶绿素b、总叶绿素、糖含量和苯丙氨酸解氨酶(PAL)的活性均显著增加。ag纳米粒子抑制了PGPR对根和茎长的影响,但增加了两个品种叶片中蛋白质和酚类物质的含量。ag纳米粒子与PGPR联合处理可使植物叶片中黄酮类化合物含量、防御相关酶PAL、抗氧化酶、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性显著升高。银纳米颗粒单独应用时也具有刺激作用。恶臭假单胞菌可以单独使用,也可以与银纳米颗粒联合使用,提高植物抗氧化和防御酶的活性,使植物更好地耐受逆境。
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引用次数: 25
Expression and Biochemical Characterization of a Yersinia intermedia Phytase Expressed in Escherichia coli. 大肠杆菌中表达的一种耶尔森菌中间植酸酶的表达及生化特性
Q1 Agricultural and Biological Sciences Pub Date : 2019-01-01 DOI: 10.2174/2212798410666181205114153
Mariana S Vieira, Vinícius V Pereira, Alice da Cunha Morales Álvares, Lais M Nogueira, William J N Lima, Paulo A Granjeiro, Daniel B Gonçalves, Mariana Campos-da-Paz, Sonia M de Freitas, Alexsandro S Galdino

Background: Phytases are enzymes capable of degrading phytic acid and used in animal feed supplementation in order to improve digestibility through the release of minerals such as phosphorus.

Objective: The main goal of this study was to express and characterize a Yersinia intermedia phytase expressed in Escherichia coli cells.

Methods: The Y. intermedia phytase gene was synthesized and overexpressed in Escherichia coli cells. The phytase recombinante (rPHY) was purified to homogeneity using a Ni-NTA column. The biochemical and biophysical properties of the rPHY were measured in order to fully characterize the recombinant enzyme. The following patents database were consulted: Espacenet, USPTO, LATIPAT, Patent Scope, WIPO and Google Patents.

Results: The results showed that the rPHY is active at 37-40ºC and presented an optimal pH and temperature of 8.0 and 40°C, respectively. The phytase rPHY was activated by Cu2+ ion and showed resistance to trypsin and pepsin, retaining 55% of the activity at the ratio of 0.02. Furthermore, the dissociation constant (Kd = 1.1150 ± 0.0087 mM), as estimated by a fluorescence binding assay, suggests a medium affinity of the enzyme with the substrate.

Conclusion: The results of this article can be considered as innovative and for this reason, they were protected by Intellectual Property Law in Brazil. Take together, the biochemical properties of the rPHY could be useful in future for its industrial application of this enzyme as an additive in the monogastric feed.

背景:植酸酶是一种能够降解植酸的酶,用于动物饲料补充中,通过释放磷等矿物质来提高消化率。目的:本研究的主要目的是表达和表征在大肠杆菌细胞中表达的耶尔森氏菌中间植酸酶。方法:合成中间芽孢杆菌植酸酶基因,并在大肠杆菌细胞中过表达。采用Ni-NTA柱纯化植酸酶重组体(rPHY)。为了充分表征重组酶,对重组酶进行了生物化学和生物物理性质的测定。查阅了下列专利数据库:Espacenet、USPTO、LATIPAT、Patent Scope、WIPO和Google patents。结果:结果表明,rPHY在37 ~ 40℃有活性,pH和温度分别为8.0℃和40℃。植酸酶rPHY被Cu2+离子激活,对胰蛋白酶和胃蛋白酶具有抗性,在比值为0.02时保持55%的活性。此外,根据荧光结合测定,解离常数(Kd = 1.1150±0.0087 mM)表明该酶与底物具有中等亲和力。结论:本文的研究成果具有一定的创新性,因此受到巴西知识产权法的保护。综上所述,rPHY的生化特性对该酶作为单胃饲料添加剂的工业应用具有重要意义。
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引用次数: 2
The Associations of Dietary Acid Load with Insulin Resistance and Type 2 Diabetes: A Systematic Review of Existing Human Studies. 膳食酸负荷与胰岛素抵抗和2型糖尿病的关系:对现有人类研究的系统回顾
Q1 Agricultural and Biological Sciences Pub Date : 2019-01-01 DOI: 10.2174/2212798410666180924142222
Hadi Emamat, Hadith Tangestani, Zahra Bahadoran, Sajjad Khalili-Moghadam, Parvin Mirmiran

Background: Type 2 diabetes is a multifactorial disorder influenced by both genetic and environmental factors and is rising dramatically throughout the world. Recently, consideration to dietary acid-base load has been raised as a nutritional indicator that could have metabolic effects.

Objective: The aim of this study was to systematically investigate the associations of dietary acid load indices with glucose/insulin homeostasis and type 2 diabetes in a patent based review.

Methods: Systematic literature review was conducted using PubMed, Scopus and Google Scholar, from inception up to 10 January 2018. All human studies publications investigated the association of dietary acid load indices (PRAL or NEAP or Pro:K) and incidence of diabetes or glucose/insulin metabolism were included.

Results: In all, 174 studies were included for the title and abstract screening. A total of 164 articles were excluded because they did not meet the inclusion criteria. Finally, 10 articles (five crosssectional studies and five cohort studies) in accordance with our inclusion criteria were chosen for further evaluations that were published between 2008 and 2017.

Conclusion: Although there are some evidence of an association between dietary acid load and type 2 diabetes, definitive declarations in this regard will be needed to intervention human studies modifying acid-base dietary intake.

背景:2型糖尿病是一种受遗传和环境因素影响的多因素疾病,在世界范围内急剧上升。近年来,人们开始考虑将膳食酸碱负荷作为一种可能具有代谢作用的营养指标。目的:本研究的目的是在一项基于专利的综述中系统地研究膳食酸负荷指数与葡萄糖/胰岛素稳态和2型糖尿病的关系。方法:使用PubMed、Scopus和Google Scholar进行系统文献综述,时间为2018年1月10日。所有研究膳食酸负荷指数(PRAL或NEAP或Pro:K)与糖尿病发病率或葡萄糖/胰岛素代谢的相关性的人类研究出版物均被纳入。结果:174项研究被纳入标题和摘要筛选。共有164篇文献因不符合纳入标准而被排除。最后,我们选择了2008年至2017年间发表的符合纳入标准的10篇文章(5篇横断面研究和5篇队列研究)进行进一步评估。结论:虽然有一些证据表明膳食酸负荷与2型糖尿病之间存在关联,但这方面的明确声明将需要干预人类研究来改变酸碱饮食摄入。
{"title":"The Associations of Dietary Acid Load with Insulin Resistance and Type 2 Diabetes: A Systematic Review of Existing Human Studies.","authors":"Hadi Emamat,&nbsp;Hadith Tangestani,&nbsp;Zahra Bahadoran,&nbsp;Sajjad Khalili-Moghadam,&nbsp;Parvin Mirmiran","doi":"10.2174/2212798410666180924142222","DOIUrl":"https://doi.org/10.2174/2212798410666180924142222","url":null,"abstract":"<p><strong>Background: </strong>Type 2 diabetes is a multifactorial disorder influenced by both genetic and environmental factors and is rising dramatically throughout the world. Recently, consideration to dietary acid-base load has been raised as a nutritional indicator that could have metabolic effects.</p><p><strong>Objective: </strong>The aim of this study was to systematically investigate the associations of dietary acid load indices with glucose/insulin homeostasis and type 2 diabetes in a patent based review.</p><p><strong>Methods: </strong>Systematic literature review was conducted using PubMed, Scopus and Google Scholar, from inception up to 10 January 2018. All human studies publications investigated the association of dietary acid load indices (PRAL or NEAP or Pro:K) and incidence of diabetes or glucose/insulin metabolism were included.</p><p><strong>Results: </strong>In all, 174 studies were included for the title and abstract screening. A total of 164 articles were excluded because they did not meet the inclusion criteria. Finally, 10 articles (five crosssectional studies and five cohort studies) in accordance with our inclusion criteria were chosen for further evaluations that were published between 2008 and 2017.</p><p><strong>Conclusion: </strong>Although there are some evidence of an association between dietary acid load and type 2 diabetes, definitive declarations in this regard will be needed to intervention human studies modifying acid-base dietary intake.</p>","PeriodicalId":21061,"journal":{"name":"Recent patents on food, nutrition & agriculture","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36519332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
Maximizing Biomass and Lipid Production in Heterotrophic Culture of Chlorella vulgaris: Techno-Economic Assessment. 小球藻异养培养中生物量和脂质产量最大化:技术经济评价。
Q1 Agricultural and Biological Sciences Pub Date : 2019-01-01 DOI: 10.2174/2212798410666180911100034
Mohammad H Morowvat, Younes Ghasemi

Background: Nowadays, chlorophycean microalgae have attained a broad-spectrum attention as a potential candidate for biomass and bioenergy production. Despite their appreciated benefits, one of major problems is their low biomass and lipid productivity. Here we investigated the heterotrophic culture in shake flasks and stirred tank bioreactor to improve the lipid and biomass production in a naturally isolated strain of Chlorella vulgaris.

Methods: A naturally isolated C. vulgaris strain was cultivated in BG-11 medium in shake flask and bioreactor. Its biochemical composition and growth kinetic parameters were investigated.

Results: The biomass productivity was improved (3.68 fold) under heterotrophic culture compared to basal autotrophic culture condition in shake flask experiment. The total lipid content increased to 44% of total Dry Cell Weight (DCW) during heterotrophic growth after 21 days. Moreover, a great Fatty Acid Methyl Esters (FAME) yield was observed under heterotrophic cultivation. Total biomass and lipid content of microalgae in bioreactor experiment increased to 4.95 and 2.18 g L-1 respectively, during 5 days of the experiment compared to its basic autotrophic culture.

Conclusion: The techno-economic aspects of exploiting C. vulgaris as a biodiesel feedstock werealso evaluated. The results imply that heterotrophic cultivation could compensate the low biomass productivity in microalgae for green energy production. Ever growing rates of established patents on application of various genetic and bioengineering-based methods have made it possible to achieve higher lipid contents with reduced total costs for microalgal biodiesel production as well.

背景:目前,绿藻微藻作为一种潜在的生物质和生物能源生产的候选者受到了广泛的关注。尽管它们有很大的好处,但主要问题之一是它们的生物量和脂质产量低。本文研究了一株天然分离的小球藻在摇瓶和搅拌槽生物反应器中的异养培养,以提高其脂质和生物量的产量。方法:在摇瓶和生物反应器中培养一株天然分离的普通葡萄球菌。对其生化组成和生长动力学参数进行了研究。结果:在摇瓶试验中,异养培养比自养培养提高了3.68倍。异养生长21天后,总脂含量增加到总干细胞重(DCW)的44%。此外,在异养培养下,脂肪酸甲酯(FAME)的产量也很高。在生物反应器实验中,微藻的总生物量和脂质含量在5 d内分别比其基本自养培养增加到4.95和2.18 g L-1。结论:从技术经济角度评价了利用淫羊藿作为生物柴油原料的可行性。结果表明,异养培养可以弥补微藻生物量生产力低的问题,以生产绿色能源。各种基于遗传和生物工程的方法的应用专利不断增长,使得微藻生物柴油生产在降低总成本的同时获得更高的脂质含量成为可能。
{"title":"Maximizing Biomass and Lipid Production in Heterotrophic Culture of Chlorella vulgaris: Techno-Economic Assessment.","authors":"Mohammad H Morowvat,&nbsp;Younes Ghasemi","doi":"10.2174/2212798410666180911100034","DOIUrl":"https://doi.org/10.2174/2212798410666180911100034","url":null,"abstract":"<p><strong>Background: </strong>Nowadays, chlorophycean microalgae have attained a broad-spectrum attention as a potential candidate for biomass and bioenergy production. Despite their appreciated benefits, one of major problems is their low biomass and lipid productivity. Here we investigated the heterotrophic culture in shake flasks and stirred tank bioreactor to improve the lipid and biomass production in a naturally isolated strain of Chlorella vulgaris.</p><p><strong>Methods: </strong>A naturally isolated C. vulgaris strain was cultivated in BG-11 medium in shake flask and bioreactor. Its biochemical composition and growth kinetic parameters were investigated.</p><p><strong>Results: </strong>The biomass productivity was improved (3.68 fold) under heterotrophic culture compared to basal autotrophic culture condition in shake flask experiment. The total lipid content increased to 44% of total Dry Cell Weight (DCW) during heterotrophic growth after 21 days. Moreover, a great Fatty Acid Methyl Esters (FAME) yield was observed under heterotrophic cultivation. Total biomass and lipid content of microalgae in bioreactor experiment increased to 4.95 and 2.18 g L-1 respectively, during 5 days of the experiment compared to its basic autotrophic culture.</p><p><strong>Conclusion: </strong>The techno-economic aspects of exploiting C. vulgaris as a biodiesel feedstock werealso evaluated. The results imply that heterotrophic cultivation could compensate the low biomass productivity in microalgae for green energy production. Ever growing rates of established patents on application of various genetic and bioengineering-based methods have made it possible to achieve higher lipid contents with reduced total costs for microalgal biodiesel production as well.</p>","PeriodicalId":21061,"journal":{"name":"Recent patents on food, nutrition & agriculture","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2174/2212798410666180911100034","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36484146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 9
期刊
Recent patents on food, nutrition & agriculture
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