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New Visions in Biological Science Vol. 4最新文献

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Determination of Fisher Perception towards Smooth-coated Otters (Lutrogale perspicillata): A Case Study in Western India Fisher对光滑水獭(Lutrogale perspicillata)感知的测定:以印度西部为例
Pub Date : 2021-10-08 DOI: 10.9734/bpi/nvbs/v4/1963c
Stephen Jonah Dias
Human-animal conflict is an important paradigm in a rapidly urbanising world. Various species, especially carnivores have to adapt to an ever-changing environment. This is amplified in riparian ecosystems, which are ecologically sensitive to change brought about by anthropogenic activities. This study aimed to assess fisher perception of Smooth Coated Otters (Lutrogale perspicillata) in an estuarine, urbanised landscape of Goa, India. Questionnaire surveys were conducted with the objective of understanding the current status of the species and fisher-otter interactions in the human-modified khazan areas of the Zuari estuary. The questionnaire was designed to procure data in four areas, namely otter presence, current and historical populations in the area; observed feeding habits; status as pests; and economic damages attributed to the species. Otter presence was reported in all the surveyed areas of the Zuari estuary. This study indicated a mixed perception of the fishers towards otters in terms of their status as pests, as well as incurred economic damages. Furthermore, the study also gave a preliminary insight into the abundance of L. perspicillata in the surveyed areas. The overall threat faced by the species in terms of fisher-otter conflict was found to be minimal in the landscape, with the absence of high degree retaliatory behaviour on the part of the fishers.
在一个快速城市化的世界里,人与动物的冲突是一个重要的范例。各种各样的物种,尤其是食肉动物,必须适应不断变化的环境。这在河岸生态系统中更为明显,因为河岸生态系统对人为活动带来的变化具有生态敏感性。本研究旨在评估印度果阿河口城市化景观中渔民对光滑水獭(Lutrogale perspicillata)的感知。通过问卷调查,了解珠江口人为改造的可赞区物种现状及鱼-水獭相互作用。设计调查表的目的是获取四个方面的数据,即该地区水獭的存在、当前和历史数量;观察食性;作为害虫的地位;以及该物种造成的经济损失。在Zuari河口的所有调查区域均报告有水獭的存在。这项研究表明,渔民对水獭的看法是一种复杂的看法,既认为水獭是有害生物,也认为水獭造成了经济损失。此外,该研究还对调查地区的糙螺旋藻丰度有了初步的认识。该物种在渔民与水獭冲突方面面临的总体威胁在景观中被发现是最小的,渔民没有高度的报复行为。
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引用次数: 2
Bauhinia variegata L. and Croton sparsiflorus L. against the Human Vector Mosquitoes 紫荆花和巴豆对人病媒蚊的防制
Pub Date : 2021-10-08 DOI: 10.9734/bpi/nvbs/v4/2045f
K. Raguvaran, Rajan Maheswaran
The aim of this was to evaluate the larvicidal activity of plant powders of Bauhinia variegata and Croton sparsiflorus against the fourth instar larvae of Aedes albopictus L., Anopheles stephensi L., and Culex quinquefasciatus Say. Derivatives of plants are enriched with active molecules with exceptional mosquitocidal properties and can be advanced as low cost environmentally friendly bio pesticides. The macerated leaf powders of B. variegata and C. sparsiflorus were tested against different mosquito species up to 24 h exposure period with the concentrations of 100, 120, 140, 160, 180, and 200 mg/100 mL of tapwater. The LC50 value of C. sparsiflorus leaf powder exhibited 136.43, 150.53 and 163.74 mg/100 mL against A. albopictus, A. stephensi, and C. quinquefasciatus Say respectively. The B. variegata leaf powder exhibited the LC50 value of 133.27, 124.99 and 135.34 mg/100 mL against A. albopictus, A. stephensi, and C. quinquefasciatus Say respectively. The present investigation clearly demonstrates that C. sparsiflorus showed cent percent mortality followed by B. variegata against different mosquito species. With further isolation and identification of bioactive molecules from B. variegata and C. sparsiflorus can be evaluated and developed novel bio pesticide against mosquitoes as well as other insect pests.
研究了紫荆植物粉和巴豆植物粉对白纹伊蚊、斯氏按蚊和致倦库蚊4龄幼虫的杀虫活性。植物衍生物富含活性分子,具有优异的杀蚊性能,可作为低成本环保型生物农药发展。以100、120、140、160、180、200 mg/100 mL自来水为浓度,对不同蚊种进行浸渍处理,实验时间为24 h。疏花库蚊叶粉对白纹伊蚊、斯氏伊蚊和致倦库蚊的LC50值分别为136.43、150.53和163.74 mg/100 mL。不同叶粉对白纹伊蚊、斯氏伊蚊和致倦库蚊的LC50值分别为133.27、124.99和135.34 mg/100 mL。本调查清楚地表明,疏花隐芽孢杆菌对不同蚊种的死亡率均为1%,其次为斑叶隐芽孢杆菌。通过进一步的分离鉴定,可以进一步评价和开发新型生物农药,以防治蚊虫和其他害虫。
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引用次数: 3
Butterfly Biodiversity of Jogimatti Forest, Chitradurga, Karnataka- India 印度卡纳塔克邦Chitradurga Jogimatti森林蝴蝶的生物多样性
Pub Date : 2021-10-08 DOI: 10.9734/bpi/nvbs/v4/2750e
S. Dhananjaya
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引用次数: 0
Studies on Bacterial Populations Concomitant with Sclerotium rolfsii sclerotia in Flooded Soil, as Estimated by 16S rRNA Gene, PCR-DGGE and Sequence Analyses 利用16S rRNA基因、PCR-DGGE和序列分析对淹水土壤中罗氏菌核伴生菌群的研究
Pub Date : 2021-10-08 DOI: 10.9734/bpi/nvbs/v4/9163d
A. Adandonon, N. Momma, Y. Hoshino, T. Makino
Objective: PCR-DGGE was used to examine the bacterial communities associated with sclerotia of Sclerotium rolfsii, the causal agent of soybean stem rot. Methodology and Results: Fungal sclerotia were buried in soil amended with organic matter and incubated under flooded conditions for 15 or 30 days in a greenhouse and in the field.  The viability and associated bacterial communities of the recovered sclerotia were investigated. In samples from soil amended with rice straw or wheat bran and flooded for 30 days, DGGE band patterns revealed the greatest bacterial diversity.  In the greenhouse, for example, the diversity index from organic amendment under 30 day flooding was greater than 1.80, whereas the index for other treatments was less than 1.5. In the field experiment, this pattern was similar. The diversity index derived from DGGE band patterns had a negative relationship with sclerotial viability or disease incidence. The nucleotide sequences of the DGGE bands revealed that members of the Clostridiaceae were dominant in samples that had been flooded for 30 days, whereas Oxalobacteraceae, Nocardiaceae, and Actinomycetaceae were major groups under unflooded conditions. Conclusion: This is the first report of a soil bacterial flora associated with S. rolfsii sclerotia under flooded conditions.
目的:采用PCR-DGGE技术对大豆茎腐病病原菌罗氏菌核菌菌核相关菌群进行研究。方法与结果:将菌核菌埋于经有机质改良的土壤中,在温室和田间淹水条件下培养15或30 d。研究了回收的菌核的生存能力和相关的细菌群落。在稻草或麦麸改良土壤并淹水30 d的样品中,DGGE谱带显示出最大的细菌多样性。以温室为例,30 d淹水条件下有机改性处理的多样性指数大于1.80,而其他处理的多样性指数均小于1.5。在田间试验中,这种模式是相似的。DGGE谱带的多样性指数与菌核活力和疾病发病率呈负相关。DGGE条带的核苷酸序列显示,在水淹30 d的样品中,梭菌科的成员占优势,而在未水淹条件下,草藻科、诺卡菌科和放线菌科的成员占主要地位。结论:本文首次报道了水淹条件下与罗氏菌核菌相关的土壤菌群。
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引用次数: 0
Molecular Analysis of Rhizopus Fungal Species on Deteriorating Historical Sandstone Monuments: An Advanced Approach 变质砂岩历史遗迹根霉真菌种类分子分析研究进展
Pub Date : 2021-10-08 DOI: 10.9734/bpi/nvbs/v4/4847f
Swati Shakya, N. Tripathi, S. Bhadauria
Sandstone  monuments  may  support  the  broad  categories  of  microorganism  that  are active  in  process  of  biodeterioration. Sandstone monuments have the compound of consortia of Bryophyte and fungi. Fungi  is considered  the most  effective  group  of microorganism causing  biodeterioration  of  sandstone  monuments. Fungal  units  grows in  the  outdoor  conditions  in  presence  of  low  amount  of  nutrition  and  water  it  can start  fungal  colonization and  the  biodeterioration  processes. Observing microorganisms on cultural heritage objects does not automatically assume that they actually change the physical properties or chemical composition of the sandstone. Our research study goals are Seclusion as well as Recognition of Fungi by utilizing molecular methods RFLP as well as RAPD. Eleven  fungal  species  were isolated  having  dominance  over sandstone  structures  of  the  different  monuments. During  the  investigation  it  was  observe  that Aspergillus  species  are  dominant  than Rhizopus  species.  Micro fungi  cause  discoloration  and  mechanical  exfoliation  of different  monuments  that  was  analyzed  production  of  dark  pigments, Hyphae penetration  and  organic  Acid  during  the  identification  using  molecular  technique. Therefore, in this review, we summarise the current knowledge about Rhizopus.
砂岩遗迹可能支持在生物退化过程中活跃的多种微生物。砂岩遗迹是苔藓植物和真菌的复合群落。真菌被认为是导致砂岩纪念碑生物退化的最有效的微生物群。真菌单位生长在室外条件下,存在少量的营养和水,它可以开始真菌定植和生物降解过程。观察文化遗产上的微生物并不能自动假设它们实际上改变了砂岩的物理性质或化学成分。我们的研究目标是利用分子方法RFLP和RAPD对真菌进行隔离和识别。11种真菌在不同纪念碑的砂岩结构中具有优势。在调查过程中,观察到曲霉种比根霉种占优势。微真菌引起不同遗迹的变色和机械剥落,在分子鉴定过程中分析了暗色素、菌丝渗透和有机酸的产生。因此,本文对根霉的研究现状进行了综述。
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引用次数: 0
A Bibliographic Summary on Hymenopteran Parasitoids Collected in Biomes in Brazil 巴西生物群系膜翅类寄生蜂文献综述
Pub Date : 2021-10-08 DOI: 10.9734/bpi/nvbs/v4/1929c
C. Marchiori
Our biomes and morphoclimatic domains are important not only as natural resources in our country but stand out as environments of great natural wealth on the planet. Parasitoids are exceedingly small insects that live for at least part of their life inside a host. This host is usually another insect (usually the pest of the crop) that ends up dying from parasitism. The aim of this study is to describe the parasitoids collected in Brazilian biomes. The mini review consists of a bibliographic summary of parasitoids of the Order Hymenoptera parasitoids collected in the following Biomes in Brazil. The research was carried out in studies related to the theme with emphasis on the quantitative aspects of the superfamily, family, subfamilies, genera, and species (Taxonomic groups). A bibliographic search was carried out that contained papers published from 2006 to May 2021.The mini review was prepared in Goiânia, Goias from June to August 2021using the Electronic Scientific Library Online (Scielo) and internet. Brazilian biomes are important not only as natural resources in our country but stand out as environments of great natural wealth on the planet. Brazil is one of the few countries in the world that hold the so-called biological megadiversity, that is, important ecosystems, still healthy.
我们的生物群落和形态气候域不仅作为我国的自然资源很重要,而且作为地球上巨大的自然财富环境脱颖而出。拟寄生物是一种非常小的昆虫,它们至少有一部分时间生活在宿主体内。这种寄主通常是另一种昆虫(通常是作物的害虫),最终因寄生而死亡。本研究的目的是描述在巴西生物群系收集的拟寄生物。本文对巴西下列生物群系中膜翅目寄生蜂的资料进行了综述。本研究主要集中在超科、科、亚科、属和种(类群)的数量方面。对2006年至2021年5月期间发表的论文进行了书目检索。该小型综述于2021年6月至8月在goi尼亚利用电子科学图书馆在线(Scielo)和互联网编写。巴西的生物群落不仅作为我们国家的自然资源很重要,而且作为地球上巨大的自然财富环境也很突出。巴西是世界上少数几个拥有所谓的生物超级多样性的国家之一,也就是说,重要的生态系统仍然健康。
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引用次数: 0
Study of Developmental and Immunogenic Consequences of Silver Nanoparticles in Drosophila 银纳米颗粒对果蝇发育和免疫原性影响的研究
Pub Date : 2021-10-08 DOI: 10.9734/bpi/nvbs/v4/4736f
Kanchan A. Phatak, P. Khanna, B. Nath
‘Silver’ is indeed a magic metal which has been a part of human kind since ancient times. On account of its strong anti-microbial activity, silver nanoparticles (AgNPs) have occupied a central position in pharmaceutical and consumer products. In spite of its large scale applications in consumer products, organismal toxicity of AgNPs is also well reported. Therefore the present study was undertaken to study developmental and immunogenic responses of laboratory synthesized AgNPs using Drosophila melanogaster as model system. Present study has dealt with the extensive characterization of AgNPs to understand their biological consequences especially genetic, chromosomal and developmental biological endpoints using Drosophila as model system. Laboratory synthesized PVP- coated AgNPs were characterized to ascertain their size, shape and surface morphology. By virtue of their direct binding with the amines, AgNPs showed dose dependent effects on various biological processes. Doses beyond 0.1 mM were found to affect adversely on life cycle, fecundity, longevity and courtship behaviour for male mate preference. Interestingly, AgNPs were also found to affect the injury- triggered melanogenesis in larvae indicating compromised innate immunity as well as melanin synthetic pathway. Same phenomenon of absence of melanization was reiterated in adults. These findings demonstrate AgNP mediated developmental and immunogenic consequences in Drosophila which is one of the best studied animal model systems.
“银”确实是一种神奇的金属,自古以来就是人类的一部分。由于其强大的抗微生物活性,银纳米颗粒(AgNPs)在制药和消费品中占据了中心地位。尽管AgNPs在消费品中的大规模应用,但其有机毒性也有很好的报道。因此,本研究以黑腹果蝇为模型系统,研究了实验室合成AgNPs的发育和免疫原性反应。目前的研究已经处理了AgNPs的广泛表征,以了解其生物学后果,特别是遗传,染色体和发育生物学终点,以果蝇为模型系统。对实验室合成的PVP包覆AgNPs进行了表征,确定了其尺寸、形状和表面形貌。由于其与胺的直接结合,AgNPs在各种生物过程中表现出剂量依赖性效应。超过0.1 mM的剂量会对生命周期、繁殖力、寿命和雄性择偶行为产生不利影响。有趣的是,AgNPs还被发现影响损伤引发的幼虫黑色素生成,这表明先天免疫和黑色素合成途径受到损害。同样的黑化现象在成人中也出现过。这些发现证明了AgNP介导的果蝇发育和免疫原性后果,果蝇是研究得最好的动物模型系统之一。
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引用次数: 1
Potential of Bioactive Compounds of Holothuria atra- Associated Bacteria as a Raw Material in Bioindustry 全息图菌相关菌活性化合物在生物工业原料中的应用潜力
Pub Date : 2021-10-08 DOI: 10.9734/bpi/nvbs/v4/4436f
D. Pringgenies, G. Santosa, Ali Djunaedi, A. Susanto
Sea cucumbers have bioactive compounds with health efficacies. These active compounds can be used as raw material products for marine aquaculture. This study aims to determine the potential of sea cucumber intestine-associated bacteria as a product of bioindustry materials through investigation of their characteristics using the GC-MS method. The solid-liquid method is used in the extraction process. Open Column Chromatography (OCC) is used in the fractionation process. Anti-bacterial sensitivity test was carried out using the Kirby-Bauer agar diffusion method. This study found 26 bacterial isolates resulting from the isolation of black sea cucumber symbionts (Holothuria atra). Of the 26 bacterial isolates, 9 bacterial isolates were found to have antibacterial activity against MDR bacteria. Two isolates were selected from the 9 test results, namely T.1.2 which formed a 7.3 mm zone of inhibition against Escherichia coli and 7.8 mm against Staphylococcus aureus and T.1.13 which was only active against Staphylococcus aureus with an inhibition zone of 8.2 mm. Identification of selected isolates showed that isolate T.1.2 matched with Bacillus manliponensis species with 97% homology and that isolate T.4.1.25 matched with Bacillus oceanisediminis with 95% homology. GC-MS analysis found Methyl hexadecanoate/methyl palmitate, Bis (2-ethylhexyl) -1,2-benzene dicarboxylate, 9-Octadecenal, Glycerol-1,3-dihexadecanoate and Diisooctyl-1,2-benzene dicarboxylate in sea cucumber intestine-associated bacteria. Diisooctyl-1,2-benzene dicarboxylate can be used as raw materials for plastic which is usable as raw materials for bioindustry. The study concluded that the bioactive compounds of sea cucumber intestine-associated bacteria found in H. atra are useful as a raw ingredient of marine aquaculture products because they contain natural ingredients for plastic raw materials.
海参含有具有保健功效的生物活性化合物。这些活性化合物可作为海洋水产养殖的原料产品。本研究旨在通过气相色谱-质谱法研究海参肠道相关菌的特性,确定其作为生物工业材料产品的潜力。萃取过程采用固液法。开柱色谱法(OCC)用于分馏过程。采用Kirby-Bauer琼脂扩散法进行抗菌敏感性试验。本研究从黑海参共生体(Holothuria atra)中分离出26株细菌。26株菌株中,9株菌株对耐多药耐药菌具有抑菌活性。从9个试验结果中筛选出2株,分别为T.1.2对大肠杆菌和金黄色葡萄球菌形成7.3 mm的抑制区和7.8 mm的抑制区,T.1.13只对金黄色葡萄球菌有活性,抑制区为8.2 mm。经鉴定,菌株T.1.2与曼利浦芽孢杆菌的同源性为97%,菌株T.4.1.25与海洋芽孢杆菌的同源性为95%。GC-MS分析在海参肠道相关菌中发现了十六酸甲酯/棕榈酸甲酯、双(2-乙基己基)-1,2-苯二甲酸酯、9-十八烯醛、甘油-1,3-二十六酸酯和二异辛基-1,2-苯二甲酸酯。二异辛基-1,2-苯二甲酸酯可作为塑料原料,可作为生物工业原料。研究结果表明,在海参肠道相关细菌中发现的生物活性化合物,由于含有塑料原料的天然成分,因此可以作为海洋水产养殖产品的原料。
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引用次数: 0
Transgenic: Why should their Adoption and Consumption in Nigeria be taken with Cautiousness 转基因:为什么在尼日利亚采用和消费转基因食品要谨慎
Pub Date : 2021-10-08 DOI: 10.9734/bpi/nvbs/v4/4183f
P. Aju
Genetic Engineering which involves the removal of genetic material from one organism and splicing it into the chromosomes of another is today set to revolutionize agriculture. It has given rise to a new set of organisms known as Genetically Modified Organisms (GMOs or Transgenic). The major advantage of GMO crops are yield increases as well as reduction in pesticide and herbicide use. Genetically Modified crops are today flourishing across the globe particularly in five leading countries namely the US, Argentina, China, Canada and Brazil. Worldwide, 181.5 million hectares were planted with GMO crops in 2014 with the US accounting for 40.28% of that average. About 5% of all canola, 13% of all corn, 31% of all cotton and 51% of soybean grown across the world today are genetically engineered. Notwithstanding their high potential caution need to be exercised in the adoption and consumption of GMO crops in Nigeria. Their health and environmental implications are yet to be subjected to long term scientific investigations. Fallouts from past scientific discoveries give credence to this call. For instance, nobody knew at the time DDT was discovered that DDT sprayed over a broad area would be bio-magnified through the food chain and concentrated hundreds of thousands of times in the human body. When CFCs were created, they were hailed as great discovery - inert compounds that were great carriers for aerosol sprays. Only when millions of tons of CFCs were liberated into air many years later was their scavenging effect on ozone in the upper atmosphere discovered. This paper therefore aims not only to highlight the benefits derivable from this new technology but also the need to exercise caution in the adoption and consumption of GM crops in Nigeria.
基因工程是指从一种生物体中提取遗传物质,并将其拼接到另一种生物体的染色体上,它将给农业带来革命性的变化。它产生了一种新的生物,被称为转基因生物(GMOs或转基因)。转基因作物的主要优点是产量增加以及减少农药和除草剂的使用。如今,转基因作物在全球范围内蓬勃发展,尤其是在五个主要国家,即美国、阿根廷、中国、加拿大和巴西。2014年,全球转基因作物种植面积为1.815亿公顷,其中美国占平均种植面积的40.28%。如今,全世界大约5%的油菜籽、13%的玉米、31%的棉花和51%的大豆都是转基因作物。尽管潜力巨大,但在尼日利亚采用和消费转基因作物时仍需谨慎。它们对健康和环境的影响还有待长期的科学调查。过去科学发现的结果证实了这一说法。例如,在DDT被发现的时候,没有人知道喷洒在大范围内的DDT会通过食物链被生物放大,并在人体内浓缩数十万倍。当氟氯化碳被发明出来时,它们被誉为伟大的发现——惰性化合物是气溶胶喷雾剂的绝佳载体。直到多年后,数百万吨氯氟烃被释放到空气中,人们才发现它们对高层大气中臭氧的清除作用。因此,本文的目的不仅在于强调这种新技术带来的好处,而且还在于强调在尼日利亚采用和消费转基因作物时需要谨慎。
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引用次数: 0
Maturation and Spawning of Green – Rough – Back Pufferfish, Lagocephalus lunaris 绿粗背河豚的成熟和产卵
Pub Date : 2021-10-08 DOI: 10.9734/bpi/nvbs/v4/4616f
R. Sirisha, Imandi, P. Y. Rao
Studies on maturation, spawning and fecundity are essential for estimation of reproductive potential of a population as well as fishery management. Maturation and spawning of the puffer fish Lagocephalus lunaris was reported from Visakhapatnam during January 2008 to December 2009 to understand reproductive potential of their population. The present observations showed that males are smaller than females. Sex-ratio indicated that females were dominant in the catches and the sex ratio for male to female was 1: 1.17. Chi-square (X2) analysis showed that there was a significant difference (p< 0.05) between sexes in their occurrence during April 2008, November 2008 and June 2009. The size at first maturity was found to be 141 mm total length for females of L. lunaris. A scale of six stages of maturity of gonads has been identified in females of L. lunaris. Spawning takes place throughout the year with peak during April to June and September to December. Gonad Somatic Index has been found to be high during January, April, June, September and December in females. Fecundity varied from 103355 to 298795 ova.
对成熟、产卵和繁殖力的研究对于估计种群的生殖潜力和渔业管理是必不可少的。2008年1月至2009年12月,对维萨卡帕特南河豚的成熟和产卵进行了报道,以了解其种群的生殖潜力。目前的观察表明,雄性比雌性小。性别比表明,雌鱼在渔获量中占优势,雌雄性别比为1:1 .17。卡方(X2)分析显示,2008年4月、2008年11月和2009年6月的发病率在性别间有显著差异(p< 0.05)。雌性月牙草初成熟时的体长为141 mm。雌性月牙草性腺的成熟有六个阶段。全年产卵,高峰期在4月至6月和9月至12月。雌性的性腺指数在1月、4月、6月、9月和12月较高。生殖力在103355 ~ 298795个卵之间变化。
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引用次数: 0
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New Visions in Biological Science Vol. 4
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